Position 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230
Sequence cttcctttgtccccaatctgggcgcgcgccggcgccccctggcggcctaaggactcggcgcgccggaagtggccagggcgggggcgacctcggctcacagcgcgcccggctattctcgcagctcaccatgGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCC
CCT--- CCC--- CCG--- CCC--- CCT--- CCG--- CCA--- CCT--- CCC--- CCA--- CCG--- ---CGG CCG--- ---AGG ---CGG CCC--- CCG--- ---TGG
CCC--- ---CGG CCC--- ---AGG ---CGG ---AGG ---CGG CCG--- ---TGG ---CGG CCG--- CCC--- ---GGG
CCA--- CCC--- ---CGG ---TGG ---CGG ---CGG ---CGG ---GGG CCC--- ---GGG
CCT--- ---GGG CCC---
---TGG ---GGG CCG---
---CGG ---GGG ---CGG
---GGG ---GGG
| Position/ Strand 
|
Guide Sequence + PAM + Restriction Enzymes
Only G- Only GG- Only A-
| Specificity Score
|
Predicted Efficiency
Main scores |
Out-of- Frame
Click score for details |
Off-targets for 0-1-2-3-4 mismatches + next to PAM
|
Genome Browser links to matches sorted by CFD off-target score
No exons. chr7 only |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 35 / rev |
CGAGTCCTTAGGCCGCCAGG GGG
Enzymes: NlaIV, AcyI, BfoI, HinP1I, BseDI, BanI, BstNI, BslI, SspDI, StyD4I
Cloning / PCR primers
|
82 | 65 | -- | -- | -- | -- | -- | 64 | -- | -- | None |
0 - 0 - 3 - 7 - 65
0 - 0 - 0 - 0 - 1 75 off-targets |
show all... |
| 233 / fw |
CCGTCTTCCCCTCCATCGTG GGG
Enzymes: BslI
Cloning / PCR primers
|
20 | 64 | -- | -- | -- | -- | -- | 60 | -- | -- | None |
1 - 4 - 12 - 14 - 103
1 - 1 - 0 - 0 - 1 134 off-targets |
show all... |
| 144 / rev |
GCCGTTGTCGACGACGAGCG CGG
Enzymes: HinP1I, BspFNI, TauI, PcsI, Fsp4HI
Cloning / PCR primers
|
86 | 62 | -- | -- | -- | -- | -- | 59 | -- | -- | None |
0 - 1 - 1 - 9 - 10
0 - 0 - 0 - 0 - 1 21 off-targets |
show all... |
| 192 / fw |
CATGTGCAAGGCCGGCTTCG CGG
Enzymes: BspFNI, MwoI, BstC8I
Cloning / PCR primers
|
25 | 61 | -- | -- | -- | -- | -- | 46 | -- | -- | None |
1 - 10 - 6 - 5 - 75
1 - 0 - 0 - 0 - 0 97 off-targets |
show all... |
| 105 / rev |
GGTGAGCTGCGAGAATAGCC GGG
Enzymes: BssHII, MwoI, NciI, MspI, HinP1I, StyD4I, BspFNI, BstC8I
Cloning / PCR primers
|
73 | 59 | -- | -- | -- | -- | -- | 46 | -- | -- | None |
0 - 0 - 4 - 29 - 120
0 - 0 - 0 - 0 - 0 153 off-targets |
show all... |
| 88 / rev |
GCCGGGCGCGCTGTGAGCCG AGG
High GC content
Enzymes: NmeAIII, BseDI, MwoI
Cloning / PCR primers
|
74 | 57 | -- | -- | -- | -- | -- | 60 | -- | -- | None |
0 - 0 - 2 - 22 - 178
0 - 0 - 1 - 1 - 1 202 off-targets |
show all... |
| 163 / fw |
GCCGCGCTCGTCGTCGACAA CGG
Enzymes: NlaIV, BceAI, Hpy166II, HincII, LmnI, SalI, FblI
Cloning / PCR primers
|
91 | 57 | -- | -- | -- | -- | -- | 64 | -- | -- | None |
0 - 0 - 0 - 14 - 14
0 - 0 - 0 - 1 - 0 28 off-targets |
show all... |
| 36 / rev |
CCGAGTCCTTAGGCCGCCAG GGG
Enzymes: NlaIV, AcyI, BfoI, HinP1I, BseDI, BanI, BstNI, BslI, SspDI, StyD4I
Cloning / PCR primers
|
87 | 56 | -- | -- | -- | -- | -- | 95 | -- | -- | None |
0 - 0 - 0 - 5 - 49
0 - 0 - 0 - 0 - 0 54 off-targets |
show all... |
| 180 / fw |
CAACGGCTCCGGCATGTGCA AGG
Enzymes: BshFI, MwoI, HpyCH4V, MspI, NaeI, Cfr10I, BstC8I
Cloning / PCR primers
|
7 | 56 | -- | -- | -- | -- | -- | 35 | -- | -- | None |
1 - 14 - 11 - 14 - 52
1 - 3 - 0 - 0 - 0 92 off-targets |
show all... |
| 126 / rev |
CGCGGCGATATCATCATCCA TGG
Enzymes: AsuHPI, BstDSI, BseDI, ErhI, NlaIII, Bsp19I
Cloning / PCR primers
|
32 | 55 | -- | -- | -- | -- | -- | 39 | -- | -- | None |
0 - 2 - 6 - 8 - 21
0 - 2 - 4 - 8 - 4 37 off-targets |
show all... |
| 129 / fw |
GCTATTCTCGCAGCTCACCA TGG
Enzymes: BstDSI, BtsCI, BseDI, ErhI, NlaIII, Bsp19I
Cloning / PCR primers
|
79 | 55 | -- | -- | -- | -- | -- | 34 | -- | -- | None |
0 - 0 - 1 - 16 - 63
0 - 0 - 0 - 1 - 3 80 off-targets |
show all... |
| 168 / rev |
GAAGCCGGCCTTGCACATGC CGG
Enzymes: MspI, NlaIII, NlaIV, XceI
Cloning / PCR primers
|
21 | 53 | -- | -- | -- | -- | -- | 49 | -- | -- | None |
2 - 11 - 7 - 18 - 99
2 - 11 - 2 - 1 - 1 137 off-targets |
show all... |
| 231 / fw |
GGCCGTCTTCCCCTCCATCG TGG
Enzymes: HinP1I, BccI, BslI
Cloning / PCR primers
|
23 | 53 | -- | -- | -- | -- | -- | 41 | -- | -- | None |
1 - 3 - 14 - 17 - 102
1 - 1 - 0 - 1 - 3 137 off-targets |
show all... |
| 76 / fw |
CGGCGCGCCGGAAGTGGCCA GGG
High GC content
Enzymes: BshFI, BalI, BstNI, BseDI, AcoI, BslI, StyD4I
Cloning / PCR primers
|
87 | 52 | -- | -- | -- | -- | -- | 47 | -- | -- | None |
0 - 0 - 1 - 5 - 52
0 - 0 - 0 - 0 - 2 58 off-targets |
show all... |
| 169 / fw |
CTCGTCGTCGACAACGGCTC CGG
Enzymes: NlaIV, MspI, BceAI, NlaIII, LmnI, XceI
Cloning / PCR primers
|
43 | 52 | -- | -- | -- | -- | -- | 59 | -- | -- | None |
0 - 1 - 13 - 4 - 13
0 - 1 - 0 - 0 - 2 31 off-targets |
show all... |
| 204 / rev |
GGAGGGGAAGACGGCCCGGG GGG
High GC content
Enzymes: LweI, BmeT110I, MwoI, MspI, BseDI, BglI, NciI, StyD4I, Cfr9I
Cloning / PCR primers
|
29 | 52 | -- | -- | -- | -- | -- | 101 | -- | -- | None |
0 - 5 - 23 - 37 - 363
0 - 0 - 0 - 1 - 1 428 off-targets |
show all... |
| 205 / rev |
TGGAGGGGAAGACGGCCCGG GGG
Enzymes: LweI, BmeT110I, MwoI, MspI, BseDI, PspPI, BglI, NciI, StyD4I, Cfr9I
Cloning / PCR primers
|
30 | 52 | -- | -- | -- | -- | -- | 97 | -- | -- | None |
1 - 4 - 12 - 42 - 196
1 - 0 - 0 - 0 - 0 255 off-targets |
|