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Contents:

PCR primers for off-targets of CGCCGGAAGTGGCCAGGGCG GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529701_F TCGTCGGCAGCGTCTGGTGAGCTGCGAGAATAGC 60.1 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529701_R GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT 60.6 1.00
mm4__chr14 42.73 Mbp_chr14_42734146_F Primer3: not found at this Tm N.d. 0.78
mm4__chr14 42.73 Mbp_chr14_42734146_R Primer3: not found at this Tm N.d. 0.78
mm3__chr20 50.04 Mbp_chr20_50040348_F TCGTCGGCAGCGTCGGGCCTGAGACTCCGTAAAC 60.1 0.58
mm3__chr20 50.04 Mbp_chr20_50040348_R GTCTCGTGGGCTCGGCCTTCCCAGGATCTCCCACA 60.6 0.58
mm4__chr16 69.90 Mbp_chr16_69901174_F Primer3: not found at this Tm N.d. 0.45
mm4__chr16 69.90 Mbp_chr16_69901174_R Primer3: not found at this Tm N.d. 0.45
mm4__chr2 241.13 Mbp_chr2_241133537_F Primer3: not found at this Tm N.d. 0.38
mm4__chr2 241.13 Mbp_chr2_241133537_R Primer3: not found at this Tm N.d. 0.38
mm4__chr4 10.08 Mbp_chr4_10078185_F Primer3: not found at this Tm N.d. 0.37
mm4__chr4 10.08 Mbp_chr4_10078185_R Primer3: not found at this Tm N.d. 0.37
mm4__chr7 6.53 Mbp_chr7_6531242_F Primer3: not found at this Tm N.d. 0.36
mm4__chr7 6.53 Mbp_chr7_6531242_R Primer3: not found at this Tm N.d. 0.36
mm3__chr1 103.96 Mbp_chr1_103961102_F Primer3: not found at this Tm N.d. 0.36
mm3__chr1 103.96 Mbp_chr1_103961102_R Primer3: not found at this Tm N.d. 0.36
mm4__chr15 27.59 Mbp_chr15_27591257_F TCGTCGGCAGCGTCAACAGCTACTGTGCACTGGG 60.2 0.35
mm4__chr15 27.59 Mbp_chr15_27591257_R GTCTCGTGGGCTCGGGACATGCTGGGGATTCACCA 60.0 0.35
mm4__chr14 75.47 Mbp_chr14_75470353_F TCGTCGGCAGCGTCAGTTACGGCCGTTGTGTGTA 59.6 0.35
mm4__chr14 75.47 Mbp_chr14_75470353_R GTCTCGTGGGCTCGGAAGGATGGAAATGCACCCCG 60.6 0.35
mm4__chr22 46.00 Mbp_chr22_45995917_F Primer3: not found at this Tm N.d. 0.34
mm4__chr22 46.00 Mbp_chr22_45995917_R Primer3: not found at this Tm N.d. 0.34
mm4__chr7 97.91 Mbp_chr7_97907437_F Primer3: not found at this Tm N.d. 0.34
mm4__chr7 97.91 Mbp_chr7_97907437_R Primer3: not found at this Tm N.d. 0.34
mm4__chr18 70.21 Mbp_chr18_70205569_F TCGTCGGCAGCGTCGGCTATCCTGACAAAGCGGG 60.8 0.34
mm4__chr18 70.21 Mbp_chr18_70205569_R GTCTCGTGGGCTCGGGTAACTGTCAGCTTGCCCCT 59.9 0.34
mm4__chr3 38.40 Mbp_chr3_38397185_F TCGTCGGCAGCGTCGTGTGGGTTCTGCATACCGA 60.0 0.33
mm4__chr3 38.40 Mbp_chr3_38397185_R GTCTCGTGGGCTCGGGGTACACCTCACTCTCCCCT 59.9 0.33
mm4__chr2 73.28 Mbp_chr2_73284695_F Primer3: not found at this Tm N.d. 0.31
mm4__chr2 73.28 Mbp_chr2_73284695_R Primer3: not found at this Tm N.d. 0.31
mm3__chr12 109.83 Mbp_chr12_109828320_F TCGTCGGCAGCGTCTCCTGTGCGCCTGGTGTG 62.6 0.30
mm3__chr12 109.83 Mbp_chr12_109828320_R GTCTCGTGGGCTCGGCCTGAGCATTGGTTTCCCCT 59.9 0.30
mm4__chr4 1.10 Mbp_chr4_1097013_F TCGTCGGCAGCGTCACAGCCTCGATCAACAGGAC 59.7 0.30
mm4__chr4 1.10 Mbp_chr4_1097013_R GTCTCGTGGGCTCGGTTTTGAGGAGGGAGCGTGTC 59.9 0.30
mm4__chr8 63.14 Mbp_chr8_63144012_F Primer3: not found at this Tm N.d. 0.29
mm4__chr8 63.14 Mbp_chr8_63144012_R Primer3: not found at this Tm N.d. 0.29
mm4__chr8 8.09 Mbp_chr8_8087601_F Primer3: not found at this Tm N.d. 0.29
mm4__chr8 8.09 Mbp_chr8_8087601_R Primer3: not found at this Tm N.d. 0.29
mm4__chr8 7.20 Mbp_chr8_7199904_F Primer3: not found at this Tm N.d. 0.29
mm4__chr8 7.20 Mbp_chr8_7199904_R Primer3: not found at this Tm N.d. 0.29
mm4__chr8 12.64 Mbp_chr8_12637568_F Primer3: not found at this Tm N.d. 0.29
mm4__chr8 12.64 Mbp_chr8_12637568_R Primer3: not found at this Tm N.d. 0.29
mm4__chr7 55.24 Mbp_chr7_55235284_F TCGTCGGCAGCGTCGGCAGGAAGGCCGATTCTAG 60.2 0.28
mm4__chr7 55.24 Mbp_chr7_55235284_R GTCTCGTGGGCTCGGTTAGTGCAGGTCAGCTGCAA 59.8 0.28
mm4__chr17 58.52 Mbp_chr17_58517845_F TCGTCGGCAGCGTCCAGGCTTGCAGTGCAGGG 61.3 0.28
mm4__chr17 58.52 Mbp_chr17_58517845_R GTCTCGTGGGCTCGGTCAGGAGAGGGGCACTGTG 60.9 0.28
mm4__chr9 135.33 Mbp_chr9_135331478_F TCGTCGGCAGCGTCCCCTGAGAGGAGAGAGCAGA 59.7 0.27
mm4__chr9 135.33 Mbp_chr9_135331478_R GTCTCGTGGGCTCGGCAGTGCAAGGCCATGAACTC 59.4 0.27
mm4__chr8 144.42 Mbp_chr8_144416550_F TCGTCGGCAGCGTCGGTACTGCCACTAGCCTCCT 60.6 0.27
mm4__chr8 144.42 Mbp_chr8_144416550_R GTCTCGTGGGCTCGGTGGAAAGGTAACAGCCCCAC 59.8 0.27
mm4__chr11 69.48 Mbp_chr11_69475130_F TCGTCGGCAGCGTCAGACAGAAGCCTGAACCTGC 59.9 0.27
mm4__chr11 69.48 Mbp_chr11_69475130_R GTCTCGTGGGCTCGGCCTCAAGGAGGCAAGGAAGG 60.0 0.27
mm4__chr10 48.31 Mbp_chr10_48307207_F TCGTCGGCAGCGTCGAAACACTTCGGCAAGGGTC 59.4 0.27
mm4__chr10 48.31 Mbp_chr10_48307207_R GTCTCGTGGGCTCGGAAATACGGTCCCTATGGGCG 59.6 0.27
mm4__chr7 1.40 Mbp_chr7_1395174_F TCGTCGGCAGCGTCGGTGCAAGAACCCAGACACT 60.1 0.26
mm4__chr7 1.40 Mbp_chr7_1395174_R GTCTCGTGGGCTCGGCTGACGTCCTGGTCCTCCA 60.6 0.26
mm4__chr6 40.89 Mbp_chr6_40891282_F TCGTCGGCAGCGTCCCAGGAGGAACAAGAGCAGG 60.0 0.26
mm4__chr6 40.89 Mbp_chr6_40891282_R GTCTCGTGGGCTCGGGAGAAAGGTCTTAGCATGAAGCAC 59.9 0.26
mm4__chr22 43.50 Mbp_chr22_43503253_F TCGTCGGCAGCGTCGGTGAGGGTGATGTCAGCTC 60.1 0.26
mm4__chr22 43.50 Mbp_chr22_43503253_R GTCTCGTGGGCTCGGGCATCTGTGCCAAACTGGTG 60.0 0.26
mm4__chr1 204.09 Mbp_chr1_204085076_F TCGTCGGCAGCGTCTGGGGAAGAAAGACGTGGTG 59.8 0.26
mm4__chr1 204.09 Mbp_chr1_204085076_R GTCTCGTGGGCTCGGAGGTAAGGAGGAAGGAGGGT 58.8 0.26
mm4__chr20 33.69 Mbp_chr20_33688196_F Primer3: not found at this Tm N.d. 0.26
mm4__chr20 33.69 Mbp_chr20_33688196_R Primer3: not found at this Tm N.d. 0.26
mm3__chr14 101.15 Mbp_chr14_101151540_F Primer3: not found at this Tm N.d. 0.25
mm3__chr14 101.15 Mbp_chr14_101151540_R Primer3: not found at this Tm N.d. 0.25
mm4__chr13 83.45 Mbp_chr13_83452526_F Primer3: not found at this Tm N.d. 0.25
mm4__chr13 83.45 Mbp_chr13_83452526_R Primer3: not found at this Tm N.d. 0.25
mm4__chr15 41.54 Mbp_chr15_41536573_F TCGTCGGCAGCGTCGATCATGCCTCCTCAGCCTC 59.9 0.23
mm4__chr15 41.54 Mbp_chr15_41536573_R GTCTCGTGGGCTCGGGCTTTGGTCCCTTCTCCTCC 60.0 0.23
mm4__chr19 917.98 Kbp_chr19_917984_F Primer3: not found at this Tm N.d. 0.22
mm4__chr19 917.98 Kbp_chr19_917984_R Primer3: not found at this Tm N.d. 0.22
mm4__chr19 10.01 Mbp_chr19_10010512_F Primer3: not found at this Tm N.d. 0.21
mm4__chr19 10.01 Mbp_chr19_10010512_R Primer3: not found at this Tm N.d. 0.21
mm4__chr9 133.38 Mbp_chr9_133376658_F TCGTCGGCAGCGTCGTCGCAACCCTGGAGCTAC 60.4 0.21
mm4__chr9 133.38 Mbp_chr9_133376658_R GTCTCGTGGGCTCGGCGGGTTCAACCTAGGAGCTG 60.1 0.21
mm3__chr15 90.20 Mbp_chr15_90201078_F TCGTCGGCAGCGTCAAACCAACGCCACTAGCAGA 59.8 0.21
mm3__chr15 90.20 Mbp_chr15_90201078_R GTCTCGTGGGCTCGGCACTGCTCAGACCCTCCCA 60.9 0.21
mm4__chr17 80.26 Mbp_chr17_80261213_F TCGTCGGCAGCGTCGAGCGCGGAGGAGGCTTT 62.4 0.21
mm4__chr17 80.26 Mbp_chr17_80261213_R GTCTCGTGGGCTCGGGCTGTCCCCTTCCACTACAC 60.0 0.21
mm4__chr11 76.65 Mbp_chr11_76653182_F TCGTCGGCAGCGTCAGGGCAGTCAAGTCACCAAG 59.8 0.20
mm4__chr11 76.65 Mbp_chr11_76653182_R GTCTCGTGGGCTCGGGGAGAATGGGGCCTGAACTC 60.1 0.20
mm3__chr22 45.65 Mbp_chr22_45648577_F TCGTCGGCAGCGTCGTTCCAACTGCACTCCGAGA 59.9 0.20
mm3__chr22 45.65 Mbp_chr22_45648577_R GTCTCGTGGGCTCGGAGCTGAGATCAGGACGAGGT 60.0 0.20
mm4__chr3 46.76 Mbp_chr3_46756746_F Primer3: not found at this Tm N.d. 0.19
mm4__chr3 46.76 Mbp_chr3_46756746_R Primer3: not found at this Tm N.d. 0.19
mm4__chr8 111.47 Mbp_chr8_111470627_F Primer3: not found at this Tm N.d. 0.19
mm4__chr8 111.47 Mbp_chr8_111470627_R Primer3: not found at this Tm N.d. 0.19
mm4__chr15 53.40 Mbp_chr15_53397339_F Primer3: not found at this Tm N.d. 0.19
mm4__chr15 53.40 Mbp_chr15_53397339_R Primer3: not found at this Tm N.d. 0.19
mm4__chr16 5.51 Mbp_chr16_5508215_F Primer3: not found at this Tm N.d. 0.19
mm4__chr16 5.51 Mbp_chr16_5508215_R Primer3: not found at this Tm N.d. 0.19
mm4__chr21 46.12 Mbp_chr21_46121933_F TCGTCGGCAGCGTCCAGCTACGTGGCCTGAGTAG 59.8 0.19
mm4__chr21 46.12 Mbp_chr21_46121933_R GTCTCGTGGGCTCGGCGTCGAGTTCTGGCTGTGA 59.7 0.19
mm4__chr2 113.28 Mbp_chr2_113283906_F Primer3: not found at this Tm N.d. 0.19
mm4__chr2 113.28 Mbp_chr2_113283906_R Primer3: not found at this Tm N.d. 0.19
mm4__chr11 1.93 Mbp_chr11_1928387_F TCGTCGGCAGCGTCTCCTAAAGGAAGCCACCCCT 60.1 0.18
mm4__chr11 1.93 Mbp_chr11_1928387_R GTCTCGTGGGCTCGGCATGGCTGCTGTCCTGAGAC 60.7 0.18
mm4__chr18 74.53 Mbp_chr18_74529966_F TCGTCGGCAGCGTCATGAGATGGTCCTGTGGCCT 60.6 0.18
mm4__chr18 74.53 Mbp_chr18_74529966_R GTCTCGTGGGCTCGGGATTGGATGGGGCTTCGGAT 59.8 0.18
mm4__chr2 241.95 Mbp_chr2_241953838_F TCGTCGGCAGCGTCCAAAGGCCTGGAGCAGATGT 60.3 0.18
mm4__chr2 241.95 Mbp_chr2_241953838_R GTCTCGTGGGCTCGGAGCGGAGACCTCAGGACAG 60.6 0.18
mm4__chr6 168.50 Mbp_chr6_168501869_F TCGTCGGCAGCGTCGGTCCGTCTTGATTCCCCAC 60.3 0.18
mm4__chr6 168.50 Mbp_chr6_168501869_R GTCTCGTGGGCTCGGAGAGCTGCCAGAGTGAGGTA 59.9 0.18
mm4__chr4 152.54 Mbp_chr4_152536213_F Primer3: not found at this Tm N.d. 0.17
mm4__chr4 152.54 Mbp_chr4_152536213_R Primer3: not found at this Tm N.d. 0.17
mm4__chr2 1.50 Mbp_chr2_1503597_F TCGTCGGCAGCGTCTCCTTGCTCTCAGAGGACCT 59.5 0.17
mm4__chr2 1.50 Mbp_chr2_1503597_R GTCTCGTGGGCTCGGGGAATTTGGCCAAGTGCAGG 60.0 0.17
mm4__chr15 28.89 Mbp_chr15_28885992_F Primer3: not found at this Tm N.d. 0.17
mm4__chr15 28.89 Mbp_chr15_28885992_R Primer3: not found at this Tm N.d. 0.17
mm4__chr15 74.59 Mbp_chr15_74589999_F TCGTCGGCAGCGTCGAGGGAGATCACCAAAGGCC 60.1 0.17
mm4__chr15 74.59 Mbp_chr15_74589999_R GTCTCGTGGGCTCGGAACATCCCCTTTCTCCAGCC 59.6 0.17
mm4__chr17 39.24 Mbp_chr17_39238769_F Primer3: not found at this Tm N.d. 0.17
mm4__chr17 39.24 Mbp_chr17_39238769_R Primer3: not found at this Tm N.d. 0.17
mm4__chr19 38.40 Mbp_chr19_38395604_F TCGTCGGCAGCGTCGGCTACGAGGATTACCGGC 60.0 0.16
mm4__chr19 38.40 Mbp_chr19_38395604_R GTCTCGTGGGCTCGGGGTCAAAGGGGCAGGTAGTG 60.3 0.16
mm4__chr8 21.63 Mbp_chr8_21626033_F Primer3: not found at this Tm N.d. 0.16
mm4__chr8 21.63 Mbp_chr8_21626033_R Primer3: not found at this Tm N.d. 0.16
mm4__chr19 4.46 Mbp_chr19_4455001_F TCGTCGGCAGCGTCCCTGGGGAGAGCGGACAG 61.4 0.16
mm4__chr19 4.46 Mbp_chr19_4455001_R GTCTCGTGGGCTCGGTCTCCTTGGTGTAGGGCTCA 59.8 0.16
mm4__chr4 146.19 Mbp_chr4_146192869_F TCGTCGGCAGCGTCACAGGACAGTGGAGAGAGCC 60.9 0.15
mm4__chr4 146.19 Mbp_chr4_146192869_R GTCTCGTGGGCTCGGCCTGCCTACAGCTGTCATCC 60.1 0.15
mm4__chr14 72.81 Mbp_chr14_72809323_F TCGTCGGCAGCGTCCATCATCGTCACACAGGGCT 60.1 0.14
mm4__chr14 72.81 Mbp_chr14_72809323_R GTCTCGTGGGCTCGGTGCCATGTTCCCCAAGGTC 59.9 0.14
mm4__chr1 115.68 Mbp_chr1_115679386_F TCGTCGGCAGCGTCGGAGGACTGTGAGAGCCATG 59.8 0.14
mm4__chr1 115.68 Mbp_chr1_115679386_R GTCTCGTGGGCTCGGATAAGCCAAGTCCACAGCCC 60.0 0.14
mm4__chr20 58.84 Mbp_chr20_58840231_F TCGTCGGCAGCGTCTCTGGCTCTCCTGCTCCAT 59.9 0.14
mm4__chr20 58.84 Mbp_chr20_58840231_R GTCTCGTGGGCTCGGGGTGGGCGTTAAGGAAGCT 60.0 0.14
mm4__chr10 68.83 Mbp_chr10_68827190_F TCGTCGGCAGCGTCTAACTTGGAACGGCCCCAAA 59.8 0.13
mm4__chr10 68.83 Mbp_chr10_68827190_R GTCTCGTGGGCTCGGTTTTCCAAGTCCTGGGGCTC 59.8 0.13
mm4__chr17 16.42 Mbp_chr17_16415808_F TCGTCGGCAGCGTCCTGCTACTGAGAAGCTCCGG 59.8 0.13
mm4__chr17 16.42 Mbp_chr17_16415808_R GTCTCGTGGGCTCGGCCTTCCCCACCCCATCATTC 60.1 0.13
mm4__chr7 150.38 Mbp_chr7_150379306_F TCGTCGGCAGCGTCCAATGGAGTGGCTGGGCG 61.1 0.13
mm4__chr7 150.38 Mbp_chr7_150379306_R GTCTCGTGGGCTCGGTTAACGGGAACTGCCACCC 59.9 0.13
mm4__chr6 164.97 Mbp_chr6_164967390_F TCGTCGGCAGCGTCAAACAGGAAAGGCAGCCTCA 59.8 0.13
mm4__chr6 164.97 Mbp_chr6_164967390_R GTCTCGTGGGCTCGGGCTGCACTAGGGAATGGCTT 60.3 0.13
mm4__chr10 23.26 Mbp_chr10_23255084_F TCGTCGGCAGCGTCACACCAGTAATGTGCCAGTG 58.1 0.13
mm4__chr10 23.26 Mbp_chr10_23255084_R GTCTCGTGGGCTCGGTAGGTGCATGAGGTTGGTCC 59.3 0.13
mm4__chr19 5.82 Mbp_chr19_5815005_F TCGTCGGCAGCGTCCACAGGCAATGTAGACGGCA 60.6 0.13
mm4__chr19 5.82 Mbp_chr19_5815005_R GTCTCGTGGGCTCGGGAGGAGAGTGAGAGGAGGGG 60.1 0.13
mm4__chr13 113.67 Mbp_chr13_113671181_F TCGTCGGCAGCGTCTCTGATGAACTTGGGGCCTG 59.6 0.12
mm4__chr13 113.67 Mbp_chr13_113671181_R GTCTCGTGGGCTCGGCTAGGAAACAGCCCAGACCG 60.1 0.12
mm4__chr19 32.68 Mbp_chr19_32675039_F TCGTCGGCAGCGTCACTCCGGAGAGCAGGGAG 60.0 0.12
mm4__chr19 32.68 Mbp_chr19_32675039_R GTCTCGTGGGCTCGGGCGATGTAGCCTTTTAAGTAAGTG 58.2 0.12
mm4__chr19 16.37 Mbp_chr19_16371664_F TCGTCGGCAGCGTCAAGGTGACTGGCTGGTTAGC 59.9 0.12
mm4__chr19 16.37 Mbp_chr19_16371664_R GTCTCGTGGGCTCGGCGATAGTGGGATCCTGCGAC 60.0 0.12
mm4__chr9 134.27 Mbp_chr9_134272810_F Primer3: not found at this Tm N.d. 0.12
mm4__chr9 134.27 Mbp_chr9_134272810_R Primer3: not found at this Tm N.d. 0.12
mm4__chr20 32.99 Mbp_chr20_32985582_F TCGTCGGCAGCGTCATCCCCTACCCTTGAAGGCT 59.9 0.12
mm4__chr20 32.99 Mbp_chr20_32985582_R GTCTCGTGGGCTCGGGTCTGCACAACACTTGCAGG 59.9 0.12
mm4__chr1 204.59 Mbp_chr1_204588835_F TCGTCGGCAGCGTCCCCCATTCCTGCATCTGGT 59.3 0.11
mm4__chr1 204.59 Mbp_chr1_204588835_R GTCTCGTGGGCTCGGGAGGAGGCTGAGGAAATGCC 60.4 0.11
mm4__chr14 93.71 Mbp_chr14_93706543_F TCGTCGGCAGCGTCTATGATGGGTTTGGGCCAGG 59.7 0.11
mm4__chr14 93.71 Mbp_chr14_93706543_R GTCTCGTGGGCTCGGTAGGCTTGGCTCAAGGGTTG 59.9 0.11
mm4__chr1 8.22 Mbp_chr1_8217375_F TCGTCGGCAGCGTCGTCAGCCAGGCCAGCTTG 61.0 0.11
mm4__chr1 8.22 Mbp_chr1_8217375_R GTCTCGTGGGCTCGGGCTGCGGGGAGAGAAGCC 62.8 0.11
mm4__chr11 70.16 Mbp_chr11_70159839_F TCGTCGGCAGCGTCAGACATCCTGGAGGCCTCTT 59.9 0.11
mm4__chr11 70.16 Mbp_chr11_70159839_R GTCTCGTGGGCTCGGCTAAGGAGAAGCCCTGCTGC 60.4 0.11
mm4__chr12 131.81 Mbp_chr12_131808950_F Primer3: not found at this Tm N.d. 0.11
mm4__chr12 131.81 Mbp_chr12_131808950_R Primer3: not found at this Tm N.d. 0.11
mm3__chr20 37.41 Mbp_chr20_37406612_F TCGTCGGCAGCGTCGTGGATGTGGGTGGGAACAG 60.6 0.11
mm3__chr20 37.41 Mbp_chr20_37406612_R GTCTCGTGGGCTCGGCTTTGTCCCGGTTGCGCTC 61.9 0.11
mm4__chr6 36.84 Mbp_chr6_36840042_F Primer3: not found at this Tm N.d. 0.11
mm4__chr6 36.84 Mbp_chr6_36840042_R Primer3: not found at this Tm N.d. 0.11
mm4__chr20 610.44 Kbp_chr20_610437_F Primer3: not found at this Tm N.d. 0.10
mm4__chr20 610.44 Kbp_chr20_610437_R Primer3: not found at this Tm N.d. 0.10
mm4__chr19 7.90 Mbp_chr19_7902906_F TCGTCGGCAGCGTCAGAAGCTGGAAGGGCACTTG 60.2 0.10
mm4__chr19 7.90 Mbp_chr19_7902906_R GTCTCGTGGGCTCGGCGGCTTCCCTCTGCTCTTAG 59.8 0.10
mm2__chr11 120.86 Mbp_chr11_120862610_F Primer3: not found at this Tm N.d. 0.10
mm2__chr11 120.86 Mbp_chr11_120862610_R Primer3: not found at this Tm N.d. 0.10
mm3__chr11 120.34 Mbp_chr11_120336286_F TCGTCGGCAGCGTCCTTCCCGAAGTCAGAGGGGA 60.6 0.09
mm3__chr11 120.34 Mbp_chr11_120336286_R GTCTCGTGGGCTCGGCCGGTTCCGGTTCTGGTTT 60.2 0.09
mm4__chr19 3.43 Mbp_chr19_3428200_F Primer3: not found at this Tm N.d. 0.09
mm4__chr19 3.43 Mbp_chr19_3428200_R Primer3: not found at this Tm N.d. 0.09
mm4__chr10 3.21 Mbp_chr10_3207520_F TCGTCGGCAGCGTCTTCCGAGGGATGGGTCCTAA 59.6 0.09
mm4__chr10 3.21 Mbp_chr10_3207520_R GTCTCGTGGGCTCGGCTGCAGCATCCGTCTCAGG 60.5 0.09
mm4__chr1 164.59 Mbp_chr1_164586554_F Primer3: not found at this Tm N.d. 0.09
mm4__chr1 164.59 Mbp_chr1_164586554_R Primer3: not found at this Tm N.d. 0.09
mm4__chr22 46.74 Mbp_chr22_46737958_F Primer3: not found at this Tm N.d. 0.09
mm4__chr22 46.74 Mbp_chr22_46737958_R Primer3: not found at this Tm N.d. 0.09
mm4__chr8 28.39 Mbp_chr8_28385674_F Primer3: not found at this Tm N.d. 0.09
mm4__chr8 28.39 Mbp_chr8_28385674_R Primer3: not found at this Tm N.d. 0.09
mm3__chr20 63.89 Mbp_chr20_63890191_F TCGTCGGCAGCGTCCTGTGCTGTCCTTCCTAGGG 59.4 0.08
mm3__chr20 63.89 Mbp_chr20_63890191_R GTCTCGTGGGCTCGGTCCTTCAACATTCTGATGGGCT 59.6 0.08
mm3__chr5 443.16 Kbp_chr5_443162_F Primer3: not found at this Tm N.d. 0.08
mm3__chr5 443.16 Kbp_chr5_443162_R Primer3: not found at this Tm N.d. 0.08
mm4__chr17 76.54 Mbp_chr17_76538233_F Primer3: not found at this Tm N.d. 0.08
mm4__chr17 76.54 Mbp_chr17_76538233_R Primer3: not found at this Tm N.d. 0.08
mm4__chr1 247.45 Mbp_chr1_247446189_F Primer3: not found at this Tm N.d. 0.08
mm4__chr1 247.45 Mbp_chr1_247446189_R Primer3: not found at this Tm N.d. 0.08
mm3__chr7 27.17 Mbp_chr7_27173687_F TCGTCGGCAGCGTCCTGAGAAAGGCGGAAGTAGC 58.3 0.08
mm3__chr7 27.17 Mbp_chr7_27173687_R GTCTCGTGGGCTCGGCGGACAAATGCCCCAAAGTC 59.7 0.08
mm4__chr16 75.25 Mbp_chr16_75250780_F TCGTCGGCAGCGTCCGACCCTGCTCTAGCTCTTG 59.8 0.07
mm4__chr16 75.25 Mbp_chr16_75250780_R GTCTCGTGGGCTCGGCTGGCCACAGGAGTCCTAGC 61.9 0.07
mm4__chr7 159.10 Mbp_chr7_159097475_F TCGTCGGCAGCGTCACAGAAGACTTACGGGAGCC 59.1 0.07
mm4__chr7 159.10 Mbp_chr7_159097475_R GTCTCGTGGGCTCGGCTAACACCCTGGGCACTCAC 60.3 0.07
mm4__chr3 50.37 Mbp_chr3_50371932_F TCGTCGGCAGCGTCGACAACTCAAGCCAGTGGGA 59.8 0.07
mm4__chr3 50.37 Mbp_chr3_50371932_R GTCTCGTGGGCTCGGGTCCAGCCTAGTCCAGCAAA 59.6 0.07
mm4__chr9 95.32 Mbp_chr9_95317265_F Primer3: not found at this Tm N.d. 0.07
mm4__chr9 95.32 Mbp_chr9_95317265_R Primer3: not found at this Tm N.d. 0.07
mm4__chr20 31.50 Mbp_chr20_31496953_F TCGTCGGCAGCGTCCCCGGAGAAACTGTCTGCAA 60.2 0.07
mm4__chr20 31.50 Mbp_chr20_31496953_R GTCTCGTGGGCTCGGCCTGGACAGATGCCAGATCC 59.8 0.07
mm4__chr19 12.95 Mbp_chr19_12945837_F TCGTCGGCAGCGTCCAAAGGCCAAGGAGGGAGTG 60.6 0.07
mm4__chr19 12.95 Mbp_chr19_12945837_R GTCTCGTGGGCTCGGACACAACATGCAACTCACGC 59.9 0.07
mm4__chr9 130.05 Mbp_chr9_130053847_F TCGTCGGCAGCGTCTCCAGGAAAACGACCACAGG 59.8 0.07
mm4__chr9 130.05 Mbp_chr9_130053847_R GTCTCGTGGGCTCGGGGCCATGTTTGTTCCAGCAT 59.3 0.07
mm4__chr9 134.97 Mbp_chr9_134967699_F TCGTCGGCAGCGTCTTTAGAGGGAGTGCGTCTGC 59.7 0.07
mm4__chr9 134.97 Mbp_chr9_134967699_R GTCTCGTGGGCTCGGCGTTTCCTGCTCTGAGCTGA 60.0 0.07
mm4__chr18 11.91 Mbp_chr18_11908384_F TCGTCGGCAGCGTCACTTCCTGTTTCCGCGGTAG 60.0 0.06
mm4__chr18 11.91 Mbp_chr18_11908384_R GTCTCGTGGGCTCGGAAGCGGCTCCGGGAAGAC 62.0 0.06
mm4__chr16 29.81 Mbp_chr16_29807877_F TCGTCGGCAGCGTCCAAGAGCTTCTCCCGGTGTG 60.6 0.06
mm4__chr16 29.81 Mbp_chr16_29807877_R GTCTCGTGGGCTCGGCTTCCCCAGCCTCAGCAG 59.7 0.06
mm4__chr5 115.17 Mbp_chr5_115169947_F Primer3: not found at this Tm N.d. 0.05
mm4__chr5 115.17 Mbp_chr5_115169947_R Primer3: not found at this Tm N.d. 0.05
mm4__chr15 57.76 Mbp_chr15_57758551_F Primer3: not found at this Tm N.d. 0.05
mm4__chr15 57.76 Mbp_chr15_57758551_R Primer3: not found at this Tm N.d. 0.05
mm4__chr1 43.37 Mbp_chr1_43367371_F TCGTCGGCAGCGTCCGGATCCCCTCCTTACTTGC 59.8 0.05
mm4__chr1 43.37 Mbp_chr1_43367371_R GTCTCGTGGGCTCGGCCATGTGTCAGGGAGGAACC 60.0 0.05
mm4__chr19 1.49 Mbp_chr19_1491011_F TCGTCGGCAGCGTCCAGCTACGTCTCGCTCCG 59.9 0.05
mm4__chr19 1.49 Mbp_chr19_1491011_R GTCTCGTGGGCTCGGGACAAAGGAGGAGGGGCC 59.3 0.05
mm4__chr7 27.10 Mbp_chr7_27097012_F TCGTCGGCAGCGTCCTACTCGACCGCCGGATTTT 60.1 0.05
mm4__chr7 27.10 Mbp_chr7_27097012_R GTCTCGTGGGCTCGGGGAGGCTCCTGGGTCTCA 59.9 0.05
mm3__chr2 3.31 Mbp_chr2_3307551_F TCGTCGGCAGCGTCAATAGAACCAGCAGCCCCAC 60.0 0.05
mm3__chr2 3.31 Mbp_chr2_3307551_R GTCTCGTGGGCTCGGGCTGCTCCTGAGGCTGTG 60.4 0.05
mm4__chr22 17.12 Mbp_chr22_17121584_F TCGTCGGCAGCGTCCTGTGGCCTGGAGGAAGC 60.0 0.05
mm4__chr22 17.12 Mbp_chr22_17121584_R GTCTCGTGGGCTCGGGACTTCCTTAGGAGTCCGCG 59.8 0.05
mm4__chr21 5.12 Mbp_chr21_5122034_F TCGTCGGCAGCGTCGCAGGAGGATCAGGAAGTGG 59.8 0.04
mm4__chr21 5.12 Mbp_chr21_5122034_R GTCTCGTGGGCTCGGCAGCGTCTGAGTCTCAGCAC 60.7 0.04
mm4__chr21 44.14 Mbp_chr21_44139995_F Primer3: not found at this Tm N.d. 0.04
mm4__chr21 44.14 Mbp_chr21_44139995_R Primer3: not found at this Tm N.d. 0.04
mm4__chr2 201.12 Mbp_chr2_201116648_F TCGTCGGCAGCGTCCTGAGTCAGCATTGCAGCAG 59.5 0.04
mm4__chr2 201.12 Mbp_chr2_201116648_R GTCTCGTGGGCTCGGAGTCAGGTTCCAGAAGCGC 60.0 0.04
mm4__chr22 41.02 Mbp_chr22_41022238_F Primer3: not found at this Tm N.d. 0.04
mm4__chr22 41.02 Mbp_chr22_41022238_R Primer3: not found at this Tm N.d. 0.04
mm4__chr11 71.25 Mbp_chr11_71252854_F Primer3: not found at this Tm N.d. 0.04
mm4__chr11 71.25 Mbp_chr11_71252854_R Primer3: not found at this Tm N.d. 0.04
mm4__chr14 100.67 Mbp_chr14_100670807_F TCGTCGGCAGCGTCGCAGCTAGAAGGGGAGAAGC 60.1 0.04
mm4__chr14 100.67 Mbp_chr14_100670807_R GTCTCGTGGGCTCGGGACTCTGCCATGGCCTTCAT 60.1 0.04
mm4__chr3 195.81 Mbp_chr3_195810657_F TCGTCGGCAGCGTCCAGAGGTAAGAAGCCCACGG 60.1 0.04
mm4__chr3 195.81 Mbp_chr3_195810657_R GTCTCGTGGGCTCGGGAAGCGACAAGGAGGAGAGG 59.8 0.04
mm4__chr11 968.17 Kbp_chr11_968170_F TCGTCGGCAGCGTCGGGTTAGGCCAGGAATCCAC 60.1 0.04
mm4__chr11 968.17 Kbp_chr11_968170_R GTCTCGTGGGCTCGGCACACCACCCCTGTTGCATA 60.2 0.04
mm4__chr9 128.20 Mbp_chr9_128203685_F Primer3: not found at this Tm N.d. 0.04
mm4__chr9 128.20 Mbp_chr9_128203685_R Primer3: not found at this Tm N.d. 0.04
mm4__chr19 460.71 Kbp_chr19_460711_F Primer3: not found at this Tm N.d. 0.04
mm4__chr19 460.71 Kbp_chr19_460711_R Primer3: not found at this Tm N.d. 0.04
mm4__chr19 40.21 Mbp_chr19_40213812_F Primer3: not found at this Tm N.d. 0.04
mm4__chr19 40.21 Mbp_chr19_40213812_R Primer3: not found at this Tm N.d. 0.04
mm4__chr3 16.26 Mbp_chr3_16264942_F TCGTCGGCAGCGTCGAAACACTGCCATGGTCAGC 59.7 0.04
mm4__chr3 16.26 Mbp_chr3_16264942_R GTCTCGTGGGCTCGGCGGAAGGGCTAGTCCTTGC 60.1 0.04
mm4__chr18 47.31 Mbp_chr18_47309837_F Primer3: not found at this Tm N.d. 0.04
mm4__chr18 47.31 Mbp_chr18_47309837_R Primer3: not found at this Tm N.d. 0.04
mm4__chr3 43.11 Mbp_chr3_43105613_F TCGTCGGCAGCGTCGAGGGCTCGAACTGGCAAG 60.7 0.03
mm4__chr3 43.11 Mbp_chr3_43105613_R GTCTCGTGGGCTCGGGTGAAGCGAGCTGAGGGAG 60.1 0.03
mm4__chr17 48.61 Mbp_chr17_48614343_F TCGTCGGCAGCGTCCATCCAGGGGAAGAGCTGTG 60.1 0.03
mm4__chr17 48.61 Mbp_chr17_48614343_R GTCTCGTGGGCTCGGAGTACGCAGACTGCAAGCTT 59.9 0.03
mm4__chr5 150.31 Mbp_chr5_150307956_F TCGTCGGCAGCGTCCTGCTCAAAGGGAGGTCGTA 59.1 0.03
mm4__chr5 150.31 Mbp_chr5_150307956_R GTCTCGTGGGCTCGGGCCTGGGTCCCATTAGGCTA 61.0 0.03
mm4__chr9 5.84 Mbp_chr9_5843975_F TCGTCGGCAGCGTCAAGGATGGGAAGAGCAGTGC 60.0 0.02
mm4__chr9 5.84 Mbp_chr9_5843975_R GTCTCGTGGGCTCGGTTCTCCCGGAAATGCCATCA 59.3 0.02
mm4__chr4 11.82 Mbp_chr4_11818763_F Primer3: not found at this Tm N.d. 0.02
mm4__chr4 11.82 Mbp_chr4_11818763_R Primer3: not found at this Tm N.d. 0.02
mm4__chr5 173.06 Mbp_chr5_173057268_F TCGTCGGCAGCGTCCGGATGGAGACTTCTGCACC 60.4 0.02
mm4__chr5 173.06 Mbp_chr5_173057268_R GTCTCGTGGGCTCGGCACTGGTCCCCTCCCATCTA 60.0 0.02
mm4__chr11 15.34 Mbp_chr11_15342298_F Primer3: not found at this Tm N.d. 0.02
mm4__chr11 15.34 Mbp_chr11_15342298_R Primer3: not found at this Tm N.d. 0.02
mm4__chr5 179.00 Mbp_chr5_178997059_F Primer3: not found at this Tm N.d. 0.02
mm4__chr5 179.00 Mbp_chr5_178997059_R Primer3: not found at this Tm N.d. 0.02
mm4__chr9 126.79 Mbp_chr9_126789582_F Primer3: not found at this Tm N.d. 0.02
mm4__chr9 126.79 Mbp_chr9_126789582_R Primer3: not found at this Tm N.d. 0.02
mm4__chr1 2.34 Mbp_chr1_2342348_F TCGTCGGCAGCGTCCGAATCAACAAGGCTGCTCG 59.9 0.02
mm4__chr1 2.34 Mbp_chr1_2342348_R GTCTCGTGGGCTCGGGTCAACGTTTCTGCTTCGGC 60.3 0.02
mm4__chr19 11.49 Mbp_chr19_11487405_F TCGTCGGCAGCGTCCATGGTGCTAGGCTGGCTAC 60.5 0.02
mm4__chr19 11.49 Mbp_chr19_11487405_R GTCTCGTGGGCTCGGGGCATTCACCTCAACATGGC 59.8 0.02
mm3__chr3 168.10 Mbp_chr3_168095659_F TCGTCGGCAGCGTCCCAGATGCCTCCTGCCTTTT 60.3 0.02
mm3__chr3 168.10 Mbp_chr3_168095659_R GTCTCGTGGGCTCGGCAACAAAGTCGTGCAGTGGG 59.9 0.02
mm4__chr18 46.10 Mbp_chr18_46099622_F Primer3: not found at this Tm N.d. 0.02
mm4__chr18 46.10 Mbp_chr18_46099622_R Primer3: not found at this Tm N.d. 0.02
mm3__chr11 117.70 Mbp_chr11_117702065_F TCGTCGGCAGCGTCAAAACTGGCTTCTCCACCCC 60.1 0.02
mm3__chr11 117.70 Mbp_chr11_117702065_R GTCTCGTGGGCTCGGAAGGAGGAAGGAGGGTGGAC 60.5 0.02
mm4__chrX 41.30 Mbp_chrX_41303231_F Primer3: not found at this Tm N.d. 0.02
mm4__chrX 41.30 Mbp_chrX_41303231_R Primer3: not found at this Tm N.d. 0.02
mm4__chr15 51.09 Mbp_chr15_51094729_F Primer3: not found at this Tm N.d. 0.01
mm4__chr15 51.09 Mbp_chr15_51094729_R Primer3: not found at this Tm N.d. 0.01
mm4__chr2 70.63 Mbp_chr2_70631865_F Primer3: not found at this Tm N.d. 0.01
mm4__chr2 70.63 Mbp_chr2_70631865_R Primer3: not found at this Tm N.d. 0.01
mm4__chr9 36.04 Mbp_chr9_36037195_F Primer3: not found at this Tm N.d. 0.01
mm4__chr9 36.04 Mbp_chr9_36037195_R Primer3: not found at this Tm N.d. 0.01
mm4__chr19 3.70 Mbp_chr19_3695616_F TCGTCGGCAGCGTCCACAGTGCCTGACCTGCAT 60.3 0.00
mm4__chr19 3.70 Mbp_chr19_3695616_R GTCTCGTGGGCTCGGACACTGGGGACGGAAAGTTC 59.8 0.00
mm4__chr8 93.73 Mbp_chr8_93734140_F TCGTCGGCAGCGTCGCCTGAAGTGCTCTGGGG 60.0 0.00
mm4__chr8 93.73 Mbp_chr8_93734140_R GTCTCGTGGGCTCGGCAGAGCATTTCCGGAGACCA 59.7 0.00
mm4__chr1 53.20 Mbp_chr1_53196802_F TCGTCGGCAGCGTCCCTACTAGTGGGCGGGGC 61.5 0.00
mm4__chr1 53.20 Mbp_chr1_53196802_R GTCTCGTGGGCTCGGAGGACTCCTGAGGCACTTCT 59.8 0.00
mm3__chr11 2.79 Mbp_chr11_2791311_F TCGTCGGCAGCGTCGCATCAATTGCGGGAGTCC 59.2 0.00
mm3__chr11 2.79 Mbp_chr11_2791311_R GTCTCGTGGGCTCGGGTTTGCCGCACAGACAAGG 60.0 0.00
mm4__chr9 93.22 Mbp_chr9_93218343_F TCGTCGGCAGCGTCCTGTGTCCCTGGTGCTATCC 59.8 0.00
mm4__chr9 93.22 Mbp_chr9_93218343_R GTCTCGTGGGCTCGGCCCTGCATCTGACCACATCA 59.7 0.00
mm4__chr8 22.06 Mbp_chr8_22056746_F TCGTCGGCAGCGTCCACATCCACACACACACCCT 60.1 0.00
mm4__chr8 22.06 Mbp_chr8_22056746_R GTCTCGTGGGCTCGGTGGGGAGTCCTCGTCTGG 59.9 0.00
mm4__chr7 121.87 Mbp_chr7_121873754_F TCGTCGGCAGCGTCGCAGCTGCGTGGGATTTG 59.8 0.00
mm4__chr7 121.87 Mbp_chr7_121873754_R GTCTCGTGGGCTCGGGTAGCCCTCCGTCTCCAGTA 60.1 0.00
mm4__chr6 48.07 Mbp_chr6_48068918_F Primer3: not found at this Tm N.d. 0.00
mm4__chr6 48.07 Mbp_chr6_48068918_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 165.86 Mbp_chr6_165855292_F TCGTCGGCAGCGTCAATGAACAGAGGCGGATGGG 60.1 0.00
mm4__chr6 165.86 Mbp_chr6_165855292_R GTCTCGTGGGCTCGGGCTACCAGGTGATCCACAGG 59.8 0.00
mm4__chr22 15.79 Mbp_chr22_15785053_F TCGTCGGCAGCGTCTGGAGTAGGAGGCAGGTCTC 60.0 0.00
mm4__chr22 15.79 Mbp_chr22_15785053_R GTCTCGTGGGCTCGGCCAGATGCCCACGAAGAAGT 60.0 0.00
mm4__chr20 34.87 Mbp_chr20_34872846_F TCGTCGGCAGCGTCCTGGCTGGCCTCGTTCTC 60.1 0.00
mm4__chr20 34.87 Mbp_chr20_34872846_R GTCTCGTGGGCTCGGGGCTGTCGCGAAGATCAGG 60.8 0.00
mm4__chr19 831.91 Kbp_chr19_831906_F TCGTCGGCAGCGTCGCCTGTGACCTCCCATGG 59.7 0.00
mm4__chr19 831.91 Kbp_chr19_831906_R GTCTCGTGGGCTCGGGCAGAGGAGAGATCGGCTTC 59.9 0.00
mm4__chr19 4.72 Mbp_chr19_4723759_F TCGTCGGCAGCGTCCTCCATGCCCGGGACTGC 62.8 0.00
mm4__chr19 4.72 Mbp_chr19_4723759_R GTCTCGTGGGCTCGGAACTTCCGGGTCAAAGGTGC 60.8 0.00
mm4__chr19 3.06 Mbp_chr19_3062485_F TCGTCGGCAGCGTCGCCCCACCGCTATTGTCTAA 59.8 0.00
mm4__chr19 3.06 Mbp_chr19_3062485_R GTCTCGTGGGCTCGGCAGGTGGAGCCGAAGCCT 61.7 0.00
mm4__chr19 1.62 Mbp_chr19_1619401_F TCGTCGGCAGCGTCGGTGAAACCTGAGGCCAGC 60.9 0.00
mm4__chr19 1.62 Mbp_chr19_1619401_R GTCTCGTGGGCTCGGCCACCCTGCAGCAGAGTAAG 60.3 0.00
mm4__chr17 68.49 Mbp_chr17_68493494_F TCGTCGGCAGCGTCCCCCGCACGTGTTCTTACT 60.0 0.00
mm4__chr17 68.49 Mbp_chr17_68493494_R GTCTCGTGGGCTCGGGGAGACAAAGTGGGAAGCGA 59.9 0.00
mm4__chr16 81.04 Mbp_chr16_81035807_F Primer3: not found at this Tm N.d. 0.00
mm4__chr16 81.04 Mbp_chr16_81035807_R Primer3: not found at this Tm N.d. 0.00
mm4__chr14 19.34 Mbp_chr14_19337560_F TCGTCGGCAGCGTCCCAGATGCCCACGAAGAAGT 60.0 0.00
mm4__chr14 19.34 Mbp_chr14_19337560_R GTCTCGTGGGCTCGGTGGAGTAGGAGGCAGGTCTC 60.0 0.00
mm4__chr14 19.06 Mbp_chr14_19062462_F TCGTCGGCAGCGTCTGGAGTAGGAGGCAGGTCTC 60.0 0.00
mm4__chr14 19.06 Mbp_chr14_19062462_R GTCTCGTGGGCTCGGCCAGATGCCCACGAAGAAGT 60.0 0.00
mm4__chr12 54.47 Mbp_chr12_54473593_F TCGTCGGCAGCGTCCACAGTCACCTTCCTCCCAC 59.9 0.00
mm4__chr12 54.47 Mbp_chr12_54473593_R GTCTCGTGGGCTCGGCTCAGCCAGAACCGAGACTC 59.8 0.00
mm4__chr12 53.05 Mbp_chr12_53047463_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 53.05 Mbp_chr12_53047463_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 132.83 Mbp_chr12_132829139_F TCGTCGGCAGCGTCCAACTTCCGGAACTGCAGGA 60.2 0.00
mm4__chr12 132.83 Mbp_chr12_132829139_R GTCTCGTGGGCTCGGGGAAGTCACGCCCACACC 60.6 0.00
mm4__chr1 56.53 Mbp_chr1_56525641_F TCGTCGGCAGCGTCACCCTGCTGCCTTTGTCTTA 59.2 0.00
mm4__chr1 56.53 Mbp_chr1_56525641_R GTCTCGTGGGCTCGGCCATCACTGTCTCTGGGCTG 60.1 0.00
mm4__chr1 27.36 Mbp_chr1_27361768_F TCGTCGGCAGCGTCATGGTTGGCAGGACTGTAGC 60.0 0.00
mm4__chr1 27.36 Mbp_chr1_27361768_R GTCTCGTGGGCTCGGCTCTACCAGCACTTCAGGCC 60.1 0.00
mm4__chr1 18.11 Mbp_chr1_18107764_F TCGTCGGCAGCGTCGAGGGGAGGGCAAGGAGG 61.0 0.00
mm4__chr1 18.11 Mbp_chr1_18107764_R GTCTCGTGGGCTCGGCCGGAGTTTGGAGCCGAAT 60.0 0.00
mm4__chr1 155.13 Mbp_chr1_155127799_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 155.13 Mbp_chr1_155127799_R Primer3: not found at this Tm N.d. 0.00
mm3__chr8 143.29 Mbp_chr8_143291346_F TCGTCGGCAGCGTCGGCAACCGATGAGCTGAAGG 61.3 0.00
mm3__chr8 143.29 Mbp_chr8_143291346_R GTCTCGTGGGCTCGGGAAACGCCAGTCTCCACCC 60.6 0.00
mm3__chr7 47.12 Mbp_chr7_47122456_F TCGTCGGCAGCGTCGCAGGATTAACACAGGACCGA 60.0 0.00
mm3__chr7 47.12 Mbp_chr7_47122456_R GTCTCGTGGGCTCGGAGCCCAGAAACACAAGCCTT 60.1 0.00
mm3__chr16 28.21 Mbp_chr16_28211955_F TCGTCGGCAGCGTCCCGACCAACAGCCCCAAC 60.9 0.00
mm3__chr16 28.21 Mbp_chr16_28211955_R GTCTCGTGGGCTCGGGGATTGGTCCGGTTCCAGC 60.7 0.00
mm2__chr3 149.75 Mbp_chr3_149752479_F TCGTCGGCAGCGTCTGTCCTACGATTTCACCCGG 59.4 0.00
mm2__chr3 149.75 Mbp_chr3_149752479_R GTCTCGTGGGCTCGGCGTCCGAGTGTAACGCGTTA 60.4 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529701 TGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT
AGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG
mm3__chr20 50.04 Mbp_chr20_50040348 GGGCCTGAGACTCCGTAAACAGCCCTGGCTCGTAAAGCTGATCAAAGCAGCAGGCCTGTCCCAGCCCTGGCCACTTCCTG
CAGGGCCCAGAGCCCTGGCCTGTGGGAGATCCTGGGAAGG
mm4__chr15 27.59 Mbp_chr15_27591257 AACAGCTACTGTGCACTGGGCTGCCCTGGCCTGGCCACTTCCAGCACCCCACTGCACATGGTGAATCCCCAGCATGTC
mm4__chr14 75.47 Mbp_chr14_75470353 AGTTACGGCCGTTGTGTGTAACTCCTAAGTACTGTAGTCTCTGGGTGTCGGGGGTGGCCAGGGCGGGGGCGGGGTGCATT
TCCATCCTT
mm4__chr18 70.21 Mbp_chr18_70205569 GGCTATCCTGACAAAGCGGGGGTGAAAGAGGGAGCGGTCGCGGAGCGGCCGGCCGCGGAAACGTGACACGACCATTCTCA
GCAAGTGGCCAGAGCGCGGGGGGTGCCTTGGGCGAGGGGCAAGCTGACAGTTAC
mm4__chr3 38.40 Mbp_chr3_38397185 GTGTGGGTTCTGCATACCGAGCTTTTCATGGTAGGTTGATGGAGGGAGACAGCTATCTCTGGAAGTGGCCAGGACATGGG
GTGGGCTGAGGAGGGTGGAAAGGGGAGAGTGAGGTGTACC
mm3__chr12 109.83 Mbp_chr12_109828320 TCCTGTGCGCCTGGTGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGAAACCAATGCTCAGG
mm4__chr4 1.10 Mbp_chr4_1097013 ACAGCCTCGATCAACAGGACAGCCCCTCGTCCTGGCCACTTCCCTCTGAGGGCTGTGCCGGACAGTGGTGAGGGCCAGGA
GGCAGAGCAGCCTGCCTGGCAAGGACACGCTCCCTCCTCAAAA
mm4__chr7 55.24 Mbp_chr7_55235284 GGCAGGAAGGCCGATTCTAGACCTCACACCCGGCGGGAGCGAGCTCTGCCCTTGCCGGAAGTGACCAGGTTGCGGTTGCG
GTTGCAGTTGCAGCTGACCTGCACTAA
mm4__chr17 58.52 Mbp_chr17_58517845 CAGGCTTGCAGTGCAGGGGGCACGGTGACTCACCCAGGCGGCGTCCGCCCGACCCTCTGAGGCCGCGCTCTTGCCACTTC
CGTCTCCCGCAGCTGCTGAGGCCGCGCAGTCCCGACCCCACCACAGTGCCCCTCTCCTGA
mm4__chr9 135.33 Mbp_chr9_135331478 CCCTGAGAGGAGAGAGCAGAGAAAGAGATGGGGGCTCTGGCTGGGGGATGAGGCCCCACGGAAGCTGCCAGGGTGTGGCA
GAAATGGCCAGGGCGGGGCAGGAGCTGAGGCTGGAGTTCATGGCCTTGCACTG
mm4__chr8 144.42 Mbp_chr8_144416550 GGTACTGCCACTAGCCTCCTCCGCCTCCTGGAGTTTGCCCAGGGCCCTTTGCTGCCGGCTGGCAGGGCGGAGCTGGCGGG
AAGAGGCCAGGGCTGGGGGACCCGTCGGGTGGGGCTGTTACCTTTCCA
mm4__chr11 69.48 Mbp_chr11_69475130 AGACAGAAGCCTGAACCTGCGTGTCGGGTTGGCACAGCCAGAGGTGGCCAGGGAGGGGTAGGTCAAGGAGGCCTTCCTTG
CCTCCTTGAGG
mm4__chr10 48.31 Mbp_chr10_48307207 GAAACACTTCGGCAAGGGTCGGACGGAGGTGTCTTTGCGGCCCGCCGCGCCCTGGCTGCCTCCGGGGCGGCGGGGAGCCG
GGGAGAGCGCTTCTGCCGGCAGGTCCCCAAGCCTGCCCCGCGCCCATAGGGACCGTATTT
mm4__chr7 1.40 Mbp_chr7_1395174 GGTGCAAGAACCCAGACACTCGAAGGAAGAGGCCAGGGAGGGGTGGTGCAGGCCCCAACGAGCCTGGGCTGCTGTGCCTG
CCCTGCGCCACAGTCTGGAGGACCAGGACGTCAG
mm4__chr6 40.89 Mbp_chr6_40891282 CCAGGAGGAACAAGAGCAGGCTTCAAGGGGCAGGGTGGAGCCACCCCCTGGCCTGTCTGCACAAGTGTCCCAGCTCCCCA
CGTCCTGGCCACTTCCTCCCACTGAGATGTGCTTCATGCTAAGACCTTTCTC
mm4__chr22 43.50 Mbp_chr22_43503253 GGTGAGGGTGATGTCAGCTCCGGTTCTATCATCCACATGGCCACGCCCTGGCCTGTTGCTGCGTGCAGGCGTTCCCTAGT
AGACCTCAGCCATCCTCACCTGAGCCTCATTTCACCAGTTTGGCACAGATGC
mm4__chr1 204.09 Mbp_chr1_204085076 TGGGGAAGAAAGACGTGGTGAATGTCAGGAGTGATGGAAGCTAAGATGAGACTTAAGGGATTCCAGGTGGGATTTAGGGC
AGGAGGTGGCCAGGGTGTGGCAACCCTCCTTCCTCCTTACCT
mm4__chr15 41.54 Mbp_chr15_41536573 GATCATGCCTCCTCAGCCTCTCTTCTGGGTCCTCATCCTCAGGCAGGCAGTGGCCAGGGCTGGGCCTGGCTCTCTTTGGA
GGAGAAGGGACCAAAGC
mm4__chr9 133.38 Mbp_chr9_133376658 GTCGCAACCCTGGAGCTACGGCCGGCGGTTCCGACCGAGGGCGGCGAGGGGCCCGCGCCCTGGCCAGTGTCGGCCTGCAG
CTCCTAGGTTGAACCCG
mm3__chr15 90.20 Mbp_chr15_90201078 AAACCAACGCCACTAGCAGAGGGTCCCAGAGGAGCCGGAAGGGGCCAGGGCCTGGGCGGCGGTGGGAGGGTCTGAGCAGT
G
mm4__chr17 80.26 Mbp_chr17_80261213 GAGCGCGGAGGAGGCTTTGCGCCCCGGCAGTGCTGCCCCCGGGAAGTGGCCGGGGCACGGGGGGTGTAGTGGAAGGGGAC
AGC
mm4__chr11 76.65 Mbp_chr11_76653182 AGGGCAGTCAAGTCACCAAGTCGCAGGGAGGGGCCAGGGCTGGGGGGCTGCTTTCAGTGCATGAGAAAGGACCTCAGGGT
CCAGAGTTCAGGCCCCATTCTCC
mm3__chr22 45.65 Mbp_chr22_45648577 GTTCCAACTGCACTCCGAGATCCCGCTCCCTGACCACTTCCGACGCCGCCAGGCCTCTCACGCCTCCGTCCCACCACACC
CCCTGACCTCGTCCTGATCTCAGCT
mm4__chr21 46.12 Mbp_chr21_46121933 CAGCTACGTGGCCTGAGTAGGGTCCTCCCCGCGTGTGGGCAGGCTTCGGGTCTCTAGGCACGTCCAGCCCCCACTGGAGG
TGGACAGGGCGAGGTTGGCCCTGCCAGGCCTCTGCTCACAGCCAGAACTCGACG
mm4__chr11 1.93 Mbp_chr11_1928387 TCCTAAAGGAAGCCACCCCTTATTAGGGTGGCCAGGGCAGGCCAGGCAGACCCCAGAGGTGGCCAGGGAGAGGTAGCAGG
GGGGTCCCAGAGGGGCCCAGTCTCAGGACAGCAGCCATG
mm4__chr18 74.53 Mbp_chr18_74529966 ATGAGATGGTCCTGTGGCCTGAACCCCTGGGGTCCGGGAGACCAGCCAGGGTGAGGCTGCTGGAGCCACGCCGGAGGAGG
TCAGGGCCTGGACGCCCTATGGAGTGGAAAAGACCAGGAGATCCGAAGCCCCATCCAATC
mm4__chr2 241.95 Mbp_chr2_241953838 CAAAGGCCTGGAGCAGATGTACCCAGGCCCCAGGGAGGAGCCGGAGGTGCCCAGGCTGTGGGGAGGAGCCGGAGGTGCCC
AGGCCGGGGGGAGGAGCCAGATGTGCCCAGGCCGGGGCTGTCCTGAGGTCTCCGCT
mm4__chr6 168.50 Mbp_chr6_168501869 GGTCCGTCTTGATTCCCCACAGAAGGTGGGAGCGTCTCCAGGCGTTTGGTCCTGAGAGGGGCTGCCTCGCCCTGGCCACA
TCCTCCTGCACATCCCTTGGGTCTTCCCGCTGGCTGCAGATACCTCACTCTGGCAGCTCT
mm4__chr2 1.50 Mbp_chr2_1503597 TCCTTGCTCTCAGAGGACCTGCCGGCCAGCTGGGAGCATCTGCCTGGGCGAAGCAGACCCTGCCCCACCCTCACCCTGGC
CCCTTCCTGCCCCCGTGTCCTGCACTTGGCCAAATTCC
mm4__chr15 74.59 Mbp_chr15_74589999 GAGGGAGATCACCAAAGGCCTAAACCTGCCCACATCCATCACCAGCGCCGCCTTCACCCTCAGGACGCAGTGAGTGGCCA
GGGCCTGGGAGAAGGAAGCTGAGGCTGGAGAAAGGGGATGTT
mm4__chr19 38.40 Mbp_chr19_38395604 GGCTACGAGGATTACCGGCGCTCCGGGCCACCCGCGCCCCTCGCCCTGTCCACCTGCGTCGTGCGCTTCGCCAAGACCGG
CGCGTTGAGGGGCGCTGCCCTGGGTCCCCCAGCGGCACTACCTGCCCCTTTGACC
mm4__chr19 4.46 Mbp_chr19_4455001 CCTGGGGAGAGCGGACAGCAGAACCAGCCTCGCCCTGGCCACTCCATGCCAGGCCTAGGGCTGTGCAAGTACGAAGAGCT
CATGGACTCGTGGCTCCACCCTGTTGAGCCCTACACCAAGGAGA
mm4__chr4 146.19 Mbp_chr4_146192869 ACAGGACAGTGGAGAGAGCCCTCTCTGATACCTGTTAGTCCCTCCTTTAAAAGATGCGGGAGGTGGCCAGGGAGTGGGGA
TTTCAGAATGTGGTGGCAGAAATCAGTTTGGCATTTATTGGATGACAGCTGTAGGCAGG
mm4__chr14 72.81 Mbp_chr14_72809323 CATCATCGTCACACAGGGCTTGGGCTGCCTGGCCCTGGCCACTGCCTGCCAACACCAGGCCTGGTCCCTCCATCATCTAG
GACCTTGGGGAACATGGCA
mm4__chr1 115.68 Mbp_chr1_115679386 GGAGGACTGTGAGAGCCATGCCTGTGACACCCAGGGCTGGCATCCCACAGTACCCGCCCCCACCCTGGGCACTTCCTGGG
ACGCCAGGACTTTGCTCTGAGGGGCTGTCAGGTGGGCTGTGGACTTGGCTTAT
mm4__chr20 58.84 Mbp_chr20_58840231 TCTGGCTCTCCTGCTCCATCGCGCTCCTCCGCGCCCTTGCCACCTCCAACGCCCGTGCCCAGCAGCGCGCGGCTGCCCAA
CAGCGCCGGAGCTTCCTTAACGCCCACC
mm4__chr10 68.83 Mbp_chr10_68827190 TAACTTGGAACGGCCCCAAAGCCAGGTCGAAGGCGAGTCTCCCCGCCCTGGGGACTCCTGGCGCTGCAGTTCGCACGGCG
GATGGACGCAGTCAGGTTCTCCAGGGAGCTGCGAGGAGCCCCAGGACTTGGAAAA
mm4__chr17 16.42 Mbp_chr17_16415808 CTGCTACTGAGAAGCTCCGGGATCCCAGCAGCCGCCACGCCCTGGCCTCAGCCTGCGGGGTAAGTCGGTGGGCAATGCTG
GGGACGTTGGAGGGGGCCGGGTCTCCAAGCCTGACAGTGAATGATGGGGTGGGGAAGG
mm4__chr7 150.38 Mbp_chr7_150379306 CAATGGAGTGGCTGGGCGGCCCCGGGGATGGCCAGGCCCGCCAGCCAATCAGGCACCCGTGCCCGCCCCGCCTGCCCCGG
GAATGGCCAGGGCCGGGAGCCCGGTGCCCAAGTCGCCCTCGGGGTGGCAGTTCCCGTTAA
mm4__chr6 164.97 Mbp_chr6_164967390 AAACAGGAAAGGCAGCCTCATTCTGCTGCATCAACAGGAGGCCAGTCTTCGCTGGGCTCCAGGCGTCACAGGCAGCAGAG
CAGCGGCGGAAGTAGACAGGGCTTGGAGAGTTATAAGCCATTCCCTAGTGCAGC
mm4__chr10 23.26 Mbp_chr10_23255084 ACACCAGTAATGTGCCAGTGGGTGGGAAGTGGCCAGGGAGAGGAGGGGCTAATAGAAACACACGAAACAAGGACCAACCT
CATGCACCTA
mm4__chr19 5.82 Mbp_chr19_5815005 CACAGGCAATGTAGACGGCACCTGGGATCCCTGCAGGGGTGGACACGGGCAGTGGTCAGGGCGGGGCCGTTCCCTCCTGA
GTCCCCCGGCCCCATCTGCTTCCCCTCCTCTCACTCTCCTC
mm4__chr13 113.67 Mbp_chr13_113671181 TCTGATGAACTTGGGGCCTGCGGAGCTGCATCCCACCTCGGCCTCGGCCTGGCCCCTTCCTGAGACGGTCTGGGCTGTTT
CCTAG
mm4__chr19 32.68 Mbp_chr19_32675039 ACTCCGGAGAGCAGGGAGCCCCTCCCCCCTCACTCTCCGGGCCCCGCCCGGCCGCCGCGCCCCGAGGCGCAGCCATCCCG
CGGGAGGGGAGGCGCAGGAGGAGGCCAGCGCGAGGACACTTACTTAAAAGGCTACATCGC
mm4__chr19 16.37 Mbp_chr19_16371664 AAGGTGACTGGCTGGTTAGCGGTAGAACTGCGCTGGCTGGTGTCACCGGGCGCTGCAGGCAGGAACCGCAACGCAGGGCC
GCGCTGGCAGGAAGTGGCAAGGGTGGGGCCGGTCGCAGGATCCCACTATCG
mm4__chr20 32.99 Mbp_chr20_32985582 ATCCCCTACCCTTGAAGGCTGAAATTGGACCCCAGCGCTGGCCACTTCCAGCCTTAACCAAGCTGCATTCAGCCCCTCCA
GCCTGCAAGTGTTGTGCAGAC
mm4__chr1 204.59 Mbp_chr1_204588835 CCCCATTCCTGCATCTGGTCCAGGCTCTGGCCACATCCGGGGCCTTGTTACAGACTTTTGGGGCATTTCCTCAGCCTCCT
C
mm4__chr14 93.71 Mbp_chr14_93706543 TATGATGGGTTTGGGCCAGGACGACTCTAGAAAACACGGTGCGATCCCCCGCCCTGGGCACTGCCAGAGTAGAGAGTTTG
CCTTCTTCAGGCCAGACAACCCTTGAGCCAAGCCTA
mm4__chr1 8.22 Mbp_chr1_8217375 GTCAGCCAGGCCAGCTTGTGGGGGAGCGGCCGCCCGCGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNGGCTTCTCTCCCCGCAGC
mm4__chr11 70.16 Mbp_chr11_70159839 AGACATCCTGGAGGCCTCTTGGCAGGACCGAGGGGTTTTCCAAGGAAAGCCCTTGTTCCCCGGAGCTGGCCAGGGAGTGG
TCCCCACGCCTCGCAGCAGGGCTTCTCCTTAG
mm3__chr20 37.41 Mbp_chr20_37406612 GTGGATGTGGGTGGGAACAGCATGTGTGCCCGTGGACCCGTGTGAGCTTGGGCTCGCACCCCCAGCGCTGGCCACTCCCG
GCGGGACCCACGTGTGCCCGGGGGCGCAGGGCCACGTGCGAGCGCAACCGGGACAAAG
mm4__chr19 7.90 Mbp_chr19_7902906 AGAAGCTGGAAGGGCACTTGGCAGAGTCTGCCTGGCCCTGGCCACCTCCCGGGTGTCCGCAAAATCAGAACTTCCTTCTC
GCATCTAAGAGCAGAGGGAAGCCG
mm3__chr11 120.34 Mbp_chr11_120336286 CTTCCCGAAGTCAGAGGGGAGGGGGCCATAAAGTCGGCGGGGGACGGGAAGCGCGAGCTCGCGCCGGACGCGGCCAGGGC
CGGGAAACCAGAACCGGAACCGG
mm4__chr10 3.21 Mbp_chr10_3207520 TTCCGAGGGATGGGTCCTAACCCTGCCCCTGGCCCTGGGAAATGCATCCCTCCCCCGGTCAGTTCATCCTGGCCTTGGCC
CCTGCCGGCGTTCTCTGCCTGCCTGAGACGGATGCTGCAG
mm3__chr20 63.89 Mbp_chr20_63890191 CTGTGCTGTCCTTCCTAGGGGTGCAGGAAGTGGACAGGGCGGAGGGTTTGAAAGAATATTGAGCCAAAGCCCAGGCTCCC
TTTGGGAATCATGTTAGCCCATCAGAATGTTGAAGGA
mm3__chr7 27.17 Mbp_chr7_27173687 CTGAGAAAGGCGGAAGTAGCCAGGCACTGGCACCCCGCTGGAGGTGCCCAGGGCGCAGCCGTCGGGCGGCGGGCCCCGCG
GGAAGGGAGCCAGTTCGGCGGCGGTGGCCGAGACTTTGGGGCATTTGTCCG
mm4__chr16 75.25 Mbp_chr16_75250780 CGACCCTGCTCTAGCTCTTGCGGGTCCCCCAACCATGGACTCCAAAGCCTTCATGTCCACACTGCAGAACCCGGCCCTGG
CCCCCTCCCGCGACACTCGCGTGCGGCTAGGACTCCTGTGGCCAG
mm4__chr7 159.10 Mbp_chr7_159097475 ACAGAAGACTTACGGGAGCCGGCCCCCTCGCGTGCCCACCACGGTGTGCTGCTGAGGCCATTCCCGGGAACGCCACACTC
CGCCCTGGCCACCTCCACCACAGAAGCAATGGCAGGGCTGTGAGTGCCCAGGGTGTTAG
mm4__chr3 50.37 Mbp_chr3_50371932 GACAACTCAAGCCAGTGGGAGTAGGGCACATCCCCCACCCCCGCCCTGGCCACTGGAGGTGGCTCTGATCCCTTTATCCC
CTTTGCTGGACTAGGCTGGAC
mm4__chr20 31.50 Mbp_chr20_31496953 CCCGGAGAAACTGTCTGCAATGTCCTGCCGGGAGAGGCCAGGGGGAGGGAACTGCTGGGACCGCAGTGGGTGAAGGAGCC
GGAGGGGCAGATCCGGGAGGATGAGGATCTGGCATCTGTCCAGG
mm4__chr19 12.95 Mbp_chr19_12945837 CAAAGGCCAAGGAGGGAGTGCGCAGGTCACGTGCGCCGGTGGTCAGCGCGCGCATTGCCTGCCCCGGAAGTGGTCGGCGC
GCGGCGCGGCGCGCCTGGGCGCTAAGATGGCGGCGGCGTGAGTTGCATGTTGTGT
mm4__chr9 130.05 Mbp_chr9_130053847 TCCAGGAAAACGACCACAGGGTCAGCGGAGCTAGCCGCCGAGCCCCGCTCCCCGGGCCCTTCCGGCGGCTGCGCCCTTTC
ACCCCGGACGTGGGCGGGAGAGGAAGCGGCTGGTGATGCTGGAACAAACATGGCC
mm4__chr9 134.97 Mbp_chr9_134967699 TTTAGAGGGAGTGCGTCTGCGTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCTGTCAGCTCA
GAGCAGGAAACG
mm4__chr18 11.91 Mbp_chr18_11908384 ACTTCCTGTTTCCGCGGTAGCGCGCGGGCCTGTCACTGAAGCCCCGCCCCGGCGACCTCAGGCGGGCGGGGAAGACCGAG
TCTTCCCGGAGCCGCTT
mm4__chr16 29.81 Mbp_chr16_29807877 CAAGAGCTTCTCCCGGTGTGCACGGGGCCTCGGCCGCCCGCTAGGCCGTGGGGAGGGAGGGACGCGACGGAGGTGGCCTG
GCCGTGGCTGGCGGGGAGGGAGGCGGCTGCTGAGGCTGGGGAAG
mm4__chr1 43.37 Mbp_chr1_43367371 CGGATCCCCTCCTTACTTGCTCCCCCACCATCGGGCTTGCTGGCCGCCCATCGCCGGCAGGGCCCAGGCCGGGGCGAGGG
CGCCAACACAGGTTCCTCCCTGACACATGG
mm4__chr19 1.49 Mbp_chr19_1491011 CAGCTACGTCTCGCTCCGGCCCGGCCAATCGGTGCCCTGGGCCGCCGCGCCCCGCCCTCTCCCGGCGGGGTCTGGGCAGC
GGCGGCCGTGGCGGAGGGCTATGCGGCGGGGGAGACGGGGCAGGCCCCTCCTCCTTTGTC
mm4__chr7 27.10 Mbp_chr7_27097012 CTACTCGACCGCCGGATTTTTAGGGGTAGGAGGCGGGGGAGAGAGATGACGCTGGCGGACGTGGCCAGCGCGGGGGCCGG
GCGGTGCGCTGCAGGCCATCTGCCGGCGCCCTGAGACCCAGGAGCCTCC
mm3__chr2 3.31 Mbp_chr2_3307551 AATAGAACCAGCAGCCCCACTTCTGCAGGAGCAGCACCAGGCAGCCAGGAAACAAGAGCCCCGCCCTGCCCACTACCTGC
GCACAGCCTCAGGAGCAGC
mm4__chr22 17.12 Mbp_chr22_17121584 CTGTGGCCTGGAGGAAGCGGGAGGAGGCGCGGGCAGCCGCCAGGGCGAGGCGACCCGGTGCGGGGGGACTCCCATGGAGC
GCGGACTCCTAAGGAAGTC
mm4__chr21 5.12 Mbp_chr21_5122034 GCAGGAGGATCAGGAAGTGGTGCAGCAGGAGGCCACCAGGGCGAGAGGGCCCACGCCCTGGAACCTTCCGGAGCAGCTGG
CAGAACCCGGGCTGCGGTGCTGAGACTCAGACGCTG
mm4__chr2 201.12 Mbp_chr2_201116648 CTGAGTCAGCATTGCAGCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTCCG
GGAAACTGCAGGTGGCCGGCAGTGGCCAGGGGATGGCGGGGGCGCTTCTGGAACCTGACT
mm4__chr14 100.67 Mbp_chr14_100670807 GCAGCTAGAAGGGGAGAAGCCGGCTTGAGTTCCCACTCCCCACGCGCCTGCCACTTCCAGCGCAGAAGCAATTTGCTTTC
ATTATGAAGGCCATGGCAGAGTC
mm4__chr3 195.81 Mbp_chr3_195810657 CAGAGGTAAGAAGCCCACGGCCAGCACCTCCCGGCCCTCTGCGCCCTGGCCGCCTCCTCCGCACCACCCTCCCGGCCGGC
CTGCCCCTCTCCTCCTTGTCGCTTC
mm4__chr11 968.17 Kbp_chr11_968170 GGGTTAGGCCAGGAATCCACGTGCTGCCACATGAGCAGCGGGCCGAGCACTGTGGCTTTCTCCTCGCGCTGGCGACTTCC
GCTGCCCCTCACGGTGCTTATGCAACAGGGGTGGTGTG
mm4__chr3 16.26 Mbp_chr3_16264942 GAAACACTGCCATGGTCAGCGGGGGTGGCCGAAGGGGTAACGCCCCAGCAGTCCGAGGCCAGCTCCGAGGGTTTAACTTC
GCCGGAACCGGCCTGGGCTCGGCATCATCAGGTCGCGCCGGGCAAGGACTAGCCCTTCCG
mm4__chr3 43.11 Mbp_chr3_43105613 GAGGGCTCGAACTGGCAAGCCCCAGGGCGTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNTGAGGGTCCACCCCCGCCCCGCCCACCTCCCGCGCCTCCCTCAGCTCGCTTCAC
mm4__chr17 48.61 Mbp_chr17_48614343 CATCCAGGGGAAGAGCTGTGTGGGCGACGGGCTCTGCTCGGAGCCCTCGGCCTCCTCGCCCAGGCCGCTGGCGGCGGCAA
GCTTGCAGTCTGCGTACT
mm4__chr5 150.31 Mbp_chr5_150307956 CTGCTCAAAGGGAGGTCGTATGAAAGGACTTGTTCTCCATGTTCTCAGAAGTTCCCGCCCTGGCCTCTTCCAACCTTGAG
AGGAATTAGCCTAATGGGACCCAGGC
mm4__chr9 5.84 Mbp_chr9_5843975 AAGGATGGGAAGAGCAGTGCATCCTGGAACCGTGGCTATGGAAAATAACGAGCACTCCTCCACCCCCTGGCCAATACCGG
CACAGACTTAATTTGAGGGGTAATGTTCTGTGAAGTATGATGGCATTTCCGGGAGAA
mm4__chr5 173.06 Mbp_chr5_173057268 CGGATGGAGACTTCTGCACCAATGGGCGTGGGGGAGGCGGTGCCGCGGGGGGGCCACTAATTGGTTAGGCTGTGCCGGAG
GTGGGCCGGGCGGGGCAGCCATGTTAGATGGGAGGGGACCAGTG
mm4__chr1 2.34 Mbp_chr1_2342348 CGAATCAACAAGGCTGCTCGGGCCAGGCCAGCCCCAAGAATAGCCGCGGCAGGCGCAGGACCTGGCCAGGGGGAGGGCAG
GCCGAAGCAGAAACGTTGAC
mm4__chr19 11.49 Mbp_chr19_11487405 CATGGTGCTAGGCTGGCTACCCTCGGGCTCTGTCTGGGTGTAGGCTGCCACACCCTCCATCATGGCACAGGGCACCTCCT
CGCCCAGTCCACTGCCGGGGACACCGCTGCCTGCTGCCATGTTGAGGTGAATGCC
mm3__chr3 168.10 Mbp_chr3_168095659 CCAGATGCCTCCTGCCTTTTTTCCTTCTTCCCACTTTTTGGCCCCGCTGCCCCCGGGCAGTTCTTGGAGTCCCGCCCTGG
CCACTCCCCGCCCTATCGCGGCGGCTCCCACTGCACGACTTTGTTG
mm3__chr11 117.70 Mbp_chr11_117702065 AAAACTGGCTTCTCCACCCCTCCTCCCAACACCTCAGTCAGGCCCTGGCCACTTCCTGTGTGGCTGCCTCTTCCCTGCGT
GGCTCCCCAGTCCACCCTCCTTCCTCCTT
mm4__chr19 3.70 Mbp_chr19_3695616 CACAGTGCCTGACCTGCATCCCGGGAGGCGGCCAGGGCGGGAGAGAATGGGGAACTTTCCGTCCCCAGTGT
mm4__chr8 93.73 Mbp_chr8_93734140 GCCTGAAGTGCTCTGGGGGTGGCCGCCGGAAGTGCTCTGGGGGAGGCCGCCTAAAATGCTCTGGAGGTGGTCTCCGGAAA
TGCTCTG
mm4__chr1 53.20 Mbp_chr1_53196802 CCTACTAGTGGGCGGGGCCTGTCAGTGAGCGGCCCCTGCCCGGAAGGAGCCAGTCCGGGGCGGAGCCGATGGCCTTACAG
GGGCCGGAAGTGGCCTGCGGGCGGAGAAGTGCCTCAGGAGTCCT
mm3__chr11 2.79 Mbp_chr11_2791311 GCATCAATTGCGGGAGTCCGGCTGGGCTGGCCGCCTGCAGGGGCCAGGGCGTGAGGGCGGTGCGGAGCCTTGTCTGTGCG
GCAAAC
mm4__chr9 93.22 Mbp_chr9_93218343 CTGTGTCCCTGGTGCTATCCAAATCTATCTTTAGGTGTCCCTGCGGGAAGTGGCCATGGGGTGGGGCTGGCCCTTTCTAG
AAACCAGATACATGATGTGGTCAGATGCAGGG
mm4__chr8 22.06 Mbp_chr8_22056746 CACATCCACACACACACCCTTGCCAGCCACACGCTATCAGCTGGAAGTGGGGAGGGCGCGGGGAGGGCGTGGGGGAGCCA
GCAGCCCGCCCGCCCTCCCGCCCCCAGACGAGGACTCCCCA
mm4__chr7 121.87 Mbp_chr7_121873754 GCAGCTGCGTGGGATTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNAGGAGACGGAGCTACTGGAGACGGAGGGCTAC
mm4__chr6 165.86 Mbp_chr6_165855292 AATGAACAGAGGCGGATGGGTGGAGCAGGTGCTGGTGAGGGCACCAGCCTTCGAGGGGACTCATAGGCGGCGCGGGAAGT
GGCGGGGGGGGGGGGGGACTCAGGGGCGCTGCCTCCTGTGGATCACCTGGTAGC
mm4__chr22 15.79 Mbp_chr22_15785053 TGGAGTAGGAGGCAGGTCTCCGCGGTAAGTGGCGGGGGCGTGGACCCCACCGGGAACCCTCCCGGCTCCTTCCCTGCCTC
TCCCTGTTTTCGTGCTTTCACTTCTTCGTGGGCATCTGG
mm4__chr20 34.87 Mbp_chr20_34872846 CTGGCTGGCCTCGTTCTCCGCCGCCATCCTCGCCCGCGCCCCCCAGCAGCGCAGCGCCTGCCGGAAGTGGCTGCGGCGGG
GGAGTGGGAGGGCGGAGGGCGGGGCCTGATCTTCGCGACAGCC
mm4__chr19 831.91 Kbp_chr19_831906 GCCTGTGACCTCCCATGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNAGAGGGGCCCTGGCTGTAATAAAGAAGCCGATCTCTCCTCTGC
mm4__chr19 4.72 Mbp_chr19_4723759 CTCCATGCCCGGGACTGCGACCCCGGAAGTGGCGGGAGCGGGGGACGACAGCCGCGGCGGACACAGGGGACCCGCCGGCT
CAGGCACCTTTGACCCGGAAGTT
mm4__chr19 3.06 Mbp_chr19_3062485 GCCCCACCGCTATTGTCTAATGGCGGCGACGCCGGCAGTGGCCGCGGCTCGGCTGCTGCGGAGGCTTCGGCTCCACCTG
mm4__chr19 1.62 Mbp_chr19_1619401 GGTGAAACCTGAGGCCAGCGCCCCGTGGCCAGGCAGCAGCGTGTGCATGTCGCCGGCTGTGCCCACGGCGTGGCTGCGGA
GCACGTGGATGGCCTCGTCCAGGTGGTCTTCTATCTTACTCTGCTGCAGGGTGG
mm4__chr17 68.49 Mbp_chr17_68493494 CCCCGCACGTGTTCTTACTTTTTTGCAGCCAAGCCTCGGAGTGGCCACTCCCGGCGCTGTAGCAATCGCTTCCCACTTTG
TCTCC
mm4__chr14 19.34 Mbp_chr14_19337560 CCAGATGCCCACGAAGAAGTGAAAGCACGAAAACAGGGAGAGGCAGGGAAGGAGCCGGGAGGGTTCCCGGTGGGGTCCAC
GCCCCCGCCACTTACCGCGGAGACCTGCCTCCTACTCCA
mm4__chr14 19.06 Mbp_chr14_19062462 TGGAGTAGGAGGCAGGTCTCCGCGGTAAGTGGCGGGGGCGTGGACCCCACCGGGAACCCTCCCGGCTCCTTCCCTGCCTC
TCCCTGTTTTCGTGCTTTCACTTCTTCGTGGGCATCTGG
mm4__chr12 54.47 Mbp_chr12_54473593 CACAGTCACCTTCCTCCCACGCAAGCCCCAGCAACGGCCACTTCCGGCGCCTAACGTTGGGAGGTGGGGCGAGGGGCCGG
CACTTCCTGTGACTGGGAGTCTCGGTTCTGGCTGAG
mm4__chr12 132.83 Mbp_chr12_132829139 CAACTTCCGGAACTGCAGGACCAACTCTTTCAGGAAGCTGAACCGAGCTTCCGGCGGAAGTGGCGTGGCCGGGGCTCGGC
ATGGGCGCGGCCGAGCTCGGTGTGGGCGTGACTTCC
mm4__chr1 56.53 Mbp_chr1_56525641 ACCCTGCTGCCTTTGTCTTAACTCCACAGCCCAGCCATGGCCACTTCCTGCTTGTAACCACAGCCCAGAGACAGTGATGG
mm4__chr1 27.36 Mbp_chr1_27361768 ATGGTTGGCAGGACTGTAGCAAGAAGTGGAGCCAGAAGTGGGCACGGCGTGGTGGCCCTCCAGGGGGCTCTGGCGTGGGC
CTGAAGTGCTGGTAGAG
mm4__chr1 18.11 Mbp_chr1_18107764 GAGGGGAGGGCAAGGAGGGCGGGGGGGCGGGGAGAGGGGGAGGGACGGAGGGAGGGCGAGAGGAGGGGGGTGCTCGCGCC
GCCGGGAGAGGCGAGCGCGAGGCAGAGAGCGCGATTCGGCTCCAAACTCCGG
mm3__chr8 143.29 Mbp_chr8_143291346 GGCAACCGATGAGCTGAAGGCGCCGCGGCTGGGCCGTCCCGCTCTCCCGCCGCCGAGCGCGCTGTCCTCCCGCTGCCCAG
AGCGTCGCCGGAAGTGCCGAGGGGGCGGGCCAGGGGTGGAGACTGGCGTTTC
mm3__chr7 47.12 Mbp_chr7_47122456 GCAGGATTAACACAGGACCGACCTCGCCATGGCCACTTCGGGCCTTCGTGACCCCAGCTGTGAAGGCCAGAGGACAGGAG
AAAAAAAATGCAATGGGGACCAGTAGAGCCAAAATAAGGCTTGTGTTTCTGGGCT
mm3__chr16 28.21 Mbp_chr16_28211955 CCGACCAACAGCCCCAACGCGCCGGAAGTGGCGAGCGCCGGGCGGCCCCACAGCGCGGCCGCCGGGCTGCGGCGCTGGAA
CCGGACCAATCC
mm2__chr3 149.75 Mbp_chr3_149752479 TGTCCTACGATTTCACCCGGCAGCGCCGACCCCGCCTTCGCCACTTCCGGCGCCCGTGTCCCGGCGCCTAGAGGCTCCGG
CGTCGCAGCGTCCGGAGCTTTGGGTTCCGGCCTACGAACTAACGCGTTACACTCGGACG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

Version 4.7 - Documentation  - Contact us - Downloads/local installation - Paper - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.