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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529572_F | TCGTCGGCAGCGTCCACGATGGAGGGGAAGACG | 59.8 | 1.00 |
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529572_R | GTCTCGTGGGCTCGGGCTATTCTCGCAGCTCACCA | 60.1 | 1.00 |
| mm1__chr2 131.63 Mbp_chr2_131626200_F | TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG | 59.8 | 1.00 |
| mm1__chr2 131.63 Mbp_chr2_131626200_R | GTCTCGTGGGCTCGGACTCCTTCTGATGCATGCCC | 60.1 | 1.00 |
| mm1__chr2 131.26 Mbp_chr2_131263617_F | TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG | 59.8 | 1.00 |
| mm1__chr2 131.26 Mbp_chr2_131263617_R | GTCTCGTGGGCTCGGCCCACGATGGAAGGGAAGAC | 60.1 | 1.00 |
| mm1__chr2 130.66 Mbp_chr2_130656811_F | TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG | 59.8 | 1.00 |
| mm1__chr2 130.66 Mbp_chr2_130656811_R | GTCTCGTGGGCTCGGCCCACGATGGAAGGGAAGAC | 60.1 | 1.00 |
| mm1__chr2 130.46 Mbp_chr2_130463858_F | TCGTCGGCAGCGTCCCCACGATGGAAGGGAAGAC | 60.1 | 1.00 |
| mm1__chr2 130.46 Mbp_chr2_130463858_R | GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG | 59.8 | 1.00 |
| mm1__chr2 130.08 Mbp_chr2_130075286_F | TCGTCGGCAGCGTCCCCACGATGGAAGGGAAGAC | 60.1 | 1.00 |
| mm1__chr2 130.08 Mbp_chr2_130075286_R | GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG | 59.8 | 1.00 |
| mm3__chr22 15.72 Mbp_chr22_15721548_F | TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG | 59.8 | 0.73 |
| mm3__chr22 15.72 Mbp_chr22_15721548_R | GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG | 60.0 | 0.73 |
| mm3__chr14 19.40 Mbp_chr14_19402823_F | TCGTCGGCAGCGTCATCATGTTCTGGTGCCTGGG | 60.0 | 0.73 |
| mm3__chr14 19.40 Mbp_chr14_19402823_R | GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG | 59.8 | 0.73 |
| mm3__chr14 19.00 Mbp_chr14_18998929_F | TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG | 59.8 | 0.73 |
| mm3__chr14 19.00 Mbp_chr14_18998929_R | GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG | 60.0 | 0.73 |
| mm2__chr19 9.52 Mbp_chr19_9518211_F | TCGTCGGCAGCGTCAGTTAACAATGGCTCCGGCA | 59.9 | 0.68 |
| mm2__chr19 9.52 Mbp_chr19_9518211_R | GTCTCGTGGGCTCGGCACGATGGAGGGGAAGATGG | 59.8 | 0.68 |
| mm3__chr2 73.90 Mbp_chr2_73901376_F | TCGTCGGCAGCGTCGTGTGAAGAGGAGACCACCG | 60.0 | 0.63 |
| mm3__chr2 73.90 Mbp_chr2_73901376_R | GTCTCGTGGGCTCGGCCCACAATGGAGGGGAAGAC | 60.0 | 0.63 |
| mm4__chr6 131.81 Mbp_chr6_131808388_F | TCGTCGGCAGCGTCGCACCGGAGAGAGGCATG | 60.2 | 0.38 |
| mm4__chr6 131.81 Mbp_chr6_131808388_R | GTCTCGTGGGCTCGGGAGAGTGAACCATCCTGCCC | 60.1 | 0.38 |
| mm4__chr10 135.21 Kbp_chr10_135208_F | TCGTCGGCAGCGTCGCCAGGGGAAGACGCGAG | 62.8 | 0.31 |
| mm4__chr10 135.21 Kbp_chr10_135208_R | GTCTCGTGGGCTCGGTACTCCACCTCAGAGAACGC | 58.8 | 0.31 |
| mm4__chr2 131.04 Mbp_chr2_131040038_F | TCGTCGGCAGCGTCTGCAGCTGAGGGTGAATCAG | 60.0 | 0.29 |
| mm4__chr2 131.04 Mbp_chr2_131040038_R | GTCTCGTGGGCTCGGATCTGGTCCTTGCTGCTCTG | 59.7 | 0.29 |
| mm4__chr16 1.26 Mbp_chr16_1262928_F | TCGTCGGCAGCGTCGCCCAGTGGTAGCTGTAGAC | 59.8 | 0.28 |
| mm4__chr16 1.26 Mbp_chr16_1262928_R | GTCTCGTGGGCTCGGCCGTCCCAGAGAATGACCTG | 59.8 | 0.28 |
| mm4__chr5 36.88 Mbp_chr5_36876922_F | TCGTCGGCAGCGTCCGCCGACAGGAGAATTGGTT | 60.6 | 0.25 |
| mm4__chr5 36.88 Mbp_chr5_36876922_R | GTCTCGTGGGCTCGGACTCACAAACACCCGACCAC | 60.4 | 0.25 |
| mm4__chr17 4.74 Mbp_chr17_4739931_F | TCGTCGGCAGCGTCGGCCCGGTGGAGAGAGTC | 61.4 | 0.22 |
| mm4__chr17 4.74 Mbp_chr17_4739931_R | GTCTCGTGGGCTCGGGGTTGTGACGTCAGGGCTG | 60.9 | 0.22 |
| mm4__chr2 241.87 Mbp_chr2_241872904_F | TCGTCGGCAGCGTCAAGAGAAGGAAGGTGGCCTG | 59.3 | 0.18 |
| mm4__chr2 241.87 Mbp_chr2_241872904_R | GTCTCGTGGGCTCGGTGATGCAGCCCTCACTAACC | 59.7 | 0.18 |
| mm4__chr9 105.04 Mbp_chr9_105044568_F | TCGTCGGCAGCGTCGCCAGTCCTAGCCTTGAGTG | 60.1 | 0.17 |
| mm4__chr9 105.04 Mbp_chr9_105044568_R | GTCTCGTGGGCTCGGGACTGGCAGTCTGGCTTGAT | 60.0 | 0.17 |
| mm4__chr4 1.45 Mbp_chr4_1449103_F | TCGTCGGCAGCGTCCATCACTGCAACGCCTCAAC | 60.1 | 0.15 |
| mm4__chr4 1.45 Mbp_chr4_1449103_R | GTCTCGTGGGCTCGGCTAAGGATGGTGCCTCACGC | 60.8 | 0.15 |
| mm4__chr5 57.48 Mbp_chr5_57482619_F | TCGTCGGCAGCGTCATCTCCCACGTAGCAGTCCT | 60.0 | 0.14 |
| mm4__chr5 57.48 Mbp_chr5_57482619_R | GTCTCGTGGGCTCGGATGGGTCAGGGATGTGCAAG | 60.0 | 0.14 |
| mm4__chr13 21.02 Mbp_chr13_21023072_F | TCGTCGGCAGCGTCCTAGTCCAGCCCAGCAATCC | 60.1 | 0.14 |
| mm4__chr13 21.02 Mbp_chr13_21023072_R | GTCTCGTGGGCTCGGCTTTGGAAAGGAGTCCCCGG | 60.3 | 0.14 |
| mm4__chr9 8.86 Mbp_chr9_8857773_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4__chr9 8.86 Mbp_chr9_8857773_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4__chr17 76.14 Mbp_chr17_76140214_F | TCGTCGGCAGCGTCCTGAACCTGGCACCTGGTG | 60.6 | 0.14 |
| mm4__chr17 76.14 Mbp_chr17_76140214_R | GTCTCGTGGGCTCGGAGTGGCTGCAGGAATTCTCC | 60.0 | 0.14 |
| mm4__chr11 1.80 Mbp_chr11_1804010_F | TCGTCGGCAGCGTCCGCCACCCACATAGAAGTCC | 60.4 | 0.13 |
| mm4__chr11 1.80 Mbp_chr11_1804010_R | GTCTCGTGGGCTCGGAACACCTCTGGCATGTGCAA | 60.4 | 0.13 |
| mm4__chr5 179.70 Mbp_chr5_179702330_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr5 179.70 Mbp_chr5_179702330_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr1 12.58 Mbp_chr1_12584341_F | TCGTCGGCAGCGTCCCCCAGCACTGTTGACCTAG | 60.0 | 0.11 |
| mm4__chr1 12.58 Mbp_chr1_12584341_R | GTCTCGTGGGCTCGGAACAGCCAAGTGTTAGGGCC | 60.5 | 0.11 |
| mm4__chr3 19.15 Mbp_chr3_19148384_F | TCGTCGGCAGCGTCCCACTGCGGGCTCTTTCC | 60.7 | 0.09 |
| mm4__chr3 19.15 Mbp_chr3_19148384_R | GTCTCGTGGGCTCGGGCCAGCTGCTGAAACCCC | 61.3 | 0.09 |
| mm4__chr17 82.75 Mbp_chr17_82752592_F | TCGTCGGCAGCGTCTTCCTAGGCGGGAGAGGG | 59.7 | 0.09 |
| mm4__chr17 82.75 Mbp_chr17_82752592_R | GTCTCGTGGGCTCGGAGAGGCTTCACCCCGTGC | 62.0 | 0.09 |
| mm4__chr5 180.21 Mbp_chr5_180209282_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr5 180.21 Mbp_chr5_180209282_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr7 27.23 Mbp_chr7_27226023_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr7 27.23 Mbp_chr7_27226023_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr1 22.54 Mbp_chr1_22537708_F | TCGTCGGCAGCGTCTACTGAGCATGCTGTCTGGG | 59.4 | 0.07 |
| mm4__chr1 22.54 Mbp_chr1_22537708_R | GTCTCGTGGGCTCGGAGTTGTGAGAAAGCTGCAAAC | 57.5 | 0.07 |
| mm4__chr1 226.08 Mbp_chr1_226083817_F | TCGTCGGCAGCGTCCAGCTCCTTTCCGTCCCG | 60.1 | 0.07 |
| mm4__chr1 226.08 Mbp_chr1_226083817_R | GTCTCGTGGGCTCGGAGGATATGCGGCACCTTCAG | 59.8 | 0.07 |
| mm4__chr4 8.43 Mbp_chr4_8428550_F | TCGTCGGCAGCGTCCCCTGACGTCATCGAACCG | 60.5 | 0.07 |
| mm4__chr4 8.43 Mbp_chr4_8428550_R | GTCTCGTGGGCTCGGTGCCTAAAAGCAGAGAAGGCA | 59.9 | 0.07 |
| mm4__chrUn_GL000220v1 118.84 Kbp_chrUn_GL000220v1_118836_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chrUn_GL000220v1 118.84 Kbp_chrUn_GL000220v1_118836_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr22_KI270733v1_random 135.70 Kbp_chr22_KI270733v1_random_135701_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr22_KI270733v1_random 135.70 Kbp_chr22_KI270733v1_random_135701_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr21 8.45 Mbp_chr21_8446628_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr21 8.45 Mbp_chr21_8446628_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr21 8.22 Mbp_chr21_8219358_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr21 8.22 Mbp_chr21_8219358_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr19 56.30 Mbp_chr19_56304338_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr19 56.30 Mbp_chr19_56304338_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr20 63.69 Mbp_chr20_63690635_F | TCGTCGGCAGCGTCCCTCCCCTACAGGCAGAGAA | 60.3 | 0.06 |
| mm4__chr20 63.69 Mbp_chr20_63690635_R | GTCTCGTGGGCTCGGGTGTCCTCCCTGCTATTGGG | 59.8 | 0.06 |
| mm4__chr6 32.85 Mbp_chr6_32853586_F | TCGTCGGCAGCGTCGTAGCAGTACTGTCCCCAGC | 59.8 | 0.05 |
| mm4__chr6 32.85 Mbp_chr6_32853586_R | GTCTCGTGGGCTCGGTGGCTAGCTCTAGGTGTCCC | 60.3 | 0.05 |
| mm4__chr12 130.42 Mbp_chr12_130424629_F | TCGTCGGCAGCGTCCTGTGCTTCACCGGGAACC | 60.9 | 0.05 |
| mm4__chr12 130.42 Mbp_chr12_130424629_R | GTCTCGTGGGCTCGGGCTGTGGGCTGCTACACAAG | 61.5 | 0.05 |
| mm4__chr4 74.67 Mbp_chr4_74671272_F | TCGTCGGCAGCGTCGCCTATTTACAGACACGGATGG | 58.8 | 0.05 |
| mm4__chr4 74.67 Mbp_chr4_74671272_R | GTCTCGTGGGCTCGGTCCAAGCTCCTGCCTTTAGC | 60.0 | 0.05 |
| mm3__chr5 77.78 Mbp_chr5_77784948_F | TCGTCGGCAGCGTCATATCACCGCGCTCGTCATT | 59.9 | 0.05 |
| mm3__chr5 77.78 Mbp_chr5_77784948_R | GTCTCGTGGGCTCGGAACGATGGAGGGGAAGACTG | 59.0 | 0.05 |
| mm4__chr6 22.57 Mbp_chr6_22570261_F | TCGTCGGCAGCGTCCTCAGCCAGAGGAGGAGGAG | 60.7 | 0.04 |
| mm4__chr6 22.57 Mbp_chr6_22570261_R | GTCTCGTGGGCTCGGGTAACTACAGGCTGTCGCGA | 59.8 | 0.04 |
| mm3__chr1 237.93 Mbp_chr1_237926892_F | TCGTCGGCAGCGTCTGACAATGGCTCCAGCATGT | 59.9 | 0.04 |
| mm3__chr1 237.93 Mbp_chr1_237926892_R | GTCTCGTGGGCTCGGCACGTAGGAGTCCTTCTGGC | 59.8 | 0.04 |
| mm4__chr2 175.00 Mbp_chr2_175004806_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr2 175.00 Mbp_chr2_175004806_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr22 37.05 Mbp_chr22_37051773_F | TCGTCGGCAGCGTCGAGGAGGATGCAGACGCC | 59.8 | 0.04 |
| mm4__chr22 37.05 Mbp_chr22_37051773_R | GTCTCGTGGGCTCGGTGGTCAGGAACACCGTGC | 59.8 | 0.04 |
| mm4__chr9 134.81 Mbp_chr9_134810346_F | TCGTCGGCAGCGTCAGGTAAGTATTGGCACGGGG | 59.4 | 0.04 |
| mm4__chr9 134.81 Mbp_chr9_134810346_R | GTCTCGTGGGCTCGGCAGCATGTCCCGCTAGTCAT | 59.8 | 0.04 |
| mm4__chr17 10.20 Mbp_chr17_10198033_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr17 10.20 Mbp_chr17_10198033_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chrX 48.83 Mbp_chrX_48826477_F | TCGTCGGCAGCGTCCTGCGCATGTCCACTCCC | 60.8 | 0.03 |
| mm4__chrX 48.83 Mbp_chrX_48826477_R | GTCTCGTGGGCTCGGGACGCTCTGACAGGCCTG | 60.1 | 0.03 |
| mm4__chr7 6.85 Mbp_chr7_6854999_F | TCGTCGGCAGCGTCTAGGTCAGGGCCTCTCACC | 59.9 | 0.03 |
| mm4__chr7 6.85 Mbp_chr7_6854999_R | GTCTCGTGGGCTCGGAGATTTTGTACTGTCCTGACTGGA | 59.4 | 0.03 |
| mm4__chr3 75.62 Mbp_chr3_75618996_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr3 75.62 Mbp_chr3_75618996_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr11 71.61 Mbp_chr11_71608060_F | TCGTCGGCAGCGTCTGTACTGTCCTGACTGGGGA | 59.5 | 0.03 |
| mm4__chr11 71.61 Mbp_chr11_71608060_R | GTCTCGTGGGCTCGGTAGGTCAGGGCCTGTCACC | 60.6 | 0.03 |
| mm4__chr11 67.71 Mbp_chr11_67711211_F | TCGTCGGCAGCGTCCCTAGGTCAGGGCCTGTCA | 60.3 | 0.03 |
| mm4__chr11 67.71 Mbp_chr11_67711211_R | GTCTCGTGGGCTCGGCTGTCCTTACTGGGGAGTCC | 58.8 | 0.03 |
| mm4__chr9 87.65 Mbp_chr9_87650719_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr9 87.65 Mbp_chr9_87650719_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr17 75.63 Mbp_chr17_75634103_F | TCGTCGGCAGCGTCCTCCAGGACGACCAACAACA | 59.8 | 0.03 |
| mm4__chr17 75.63 Mbp_chr17_75634103_R | GTCTCGTGGGCTCGGCACGTGCTGTCCTCGTACTT | 60.0 | 0.03 |
| mm4__chr10 69.02 Mbp_chr10_69022815_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr10 69.02 Mbp_chr10_69022815_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr15 61.07 Mbp_chr15_61066706_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr15 61.07 Mbp_chr15_61066706_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3__chr3 175.98 Mbp_chr3_175978494_F | TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC | 60.1 | 0.02 |
| mm3__chr3 175.98 Mbp_chr3_175978494_R | GTCTCGTGGGCTCGGGCAAAGGCTCCTGTATGTGC | 59.5 | 0.02 |
| mm4__chr4 1.37 Mbp_chr4_1365978_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr4 1.37 Mbp_chr4_1365978_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr11 124.76 Mbp_chr11_124762905_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr11 124.76 Mbp_chr11_124762905_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr22 19.45 Mbp_chr22_19447974_F | TCGTCGGCAGCGTCGGGGTCGCAGGGTAAATCC | 60.1 | 0.02 |
| mm4__chr22 19.45 Mbp_chr22_19447974_R | GTCTCGTGGGCTCGGTGATTTGAGAGGCGGCTGAC | 60.3 | 0.02 |
| mm4__chr22 20.36 Mbp_chr22_20359162_F | TCGTCGGCAGCGTCCTAACGAGGTCGCTGCCC | 60.2 | 0.02 |
| mm4__chr22 20.36 Mbp_chr22_20359162_R | GTCTCGTGGGCTCGGGTCCTCGTTAGCGATGCCC | 60.5 | 0.02 |
| mm4__chr22 20.35 Mbp_chr22_20354934_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr22 20.35 Mbp_chr22_20354934_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr17 29.59 Mbp_chr17_29589797_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr17 29.59 Mbp_chr17_29589797_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr22 38.46 Mbp_chr22_38455304_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr22 38.46 Mbp_chr22_38455304_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr6 131.63 Mbp_chr6_131628374_F | TCGTCGGCAGCGTCGTTCCGTCTGTGGAGAAGGG | 60.0 | 0.01 |
| mm4__chr6 131.63 Mbp_chr6_131628374_R | GTCTCGTGGGCTCGGAAGCAACTCCTTCTCCAGCC | 59.9 | 0.01 |
| mm4__chrY 345.05 Kbp_chrY_345052_F | TCGTCGGCAGCGTCGATGACTCTTAGAGGGGCCG | 59.3 | 0.01 |
| mm4__chrY 345.05 Kbp_chrY_345052_R | GTCTCGTGGGCTCGGAGTTTCCATCCAATCCCGGG | 59.7 | 0.01 |
| mm4__chr16 1.26 Mbp_chr16_1263519_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr16 1.26 Mbp_chr16_1263519_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3__chr22 41.08 Mbp_chr22_41075154_F | TCGTCGGCAGCGTCGGAGTCCTTCTGACCCATGC | 60.1 | 0.01 |
| mm3__chr22 41.08 Mbp_chr22_41075154_R | GTCTCGTGGGCTCGGTGTTGACAACAGCTCTGGCA | 60.1 | 0.01 |
| mm4__chr6 100.88 Mbp_chr6_100881496_F | TCGTCGGCAGCGTCCTGGGTTCGGAGGAGCAGA | 60.9 | 0.01 |
| mm4__chr6 100.88 Mbp_chr6_100881496_R | GTCTCGTGGGCTCGGAGAGAGGTAGAGACGGCAGG | 60.1 | 0.01 |
| mm4__chr11 66.70 Mbp_chr11_66701055_F | TCGTCGGCAGCGTCTTGCCTCTCGAGTCAGTTCG | 59.7 | 0.01 |
| mm4__chr11 66.70 Mbp_chr11_66701055_R | GTCTCGTGGGCTCGGAAGTCATCGAGTCCACCCAG | 59.1 | 0.01 |
| mm4__chr20 4.25 Mbp_chr20_4248842_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr20 4.25 Mbp_chr20_4248842_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr19 5.11 Mbp_chr19_5109759_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr19 5.11 Mbp_chr19_5109759_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr1 227.45 Mbp_chr1_227447817_F | TCGTCGGCAGCGTCCAGTTCCAAAAGGCTGGGGA | 60.1 | 0.01 |
| mm4__chr1 227.45 Mbp_chr1_227447817_R | GTCTCGTGGGCTCGGGAGGTGGCAGAGCAGGAAAA | 60.2 | 0.01 |
| mm4__chr16 75.15 Mbp_chr16_75148974_F | TCGTCGGCAGCGTCTCTTTGTCAGGAGATGCGCC | 60.3 | 0.01 |
| mm4__chr16 75.15 Mbp_chr16_75148974_R | GTCTCGTGGGCTCGGCAGGTGTGTCAGGCCACG | 60.6 | 0.01 |
| mm4__chr22 18.19 Mbp_chr22_18186220_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr22 18.19 Mbp_chr22_18186220_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr3 14.51 Mbp_chr3_14514349_F | TCGTCGGCAGCGTCACTGTCAGGCTCGATGAAGC | 60.1 | 0.00 |
| mm4__chr3 14.51 Mbp_chr3_14514349_R | GTCTCGTGGGCTCGGAGATTGTGCACACGGAGACC | 60.3 | 0.00 |
| mm4__chr1 11.48 Mbp_chr1_11480505_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 11.48 Mbp_chr1_11480505_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr16 75.27 Mbp_chr16_75265856_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr16 75.27 Mbp_chr16_75265856_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 185.32 Mbp_chr1_185316570_F | TCGTCGGCAGCGTCTTCCTGACAGCAAGAACGGG | 60.2 | 0.00 |
| mm4__chr1 185.32 Mbp_chr1_185316570_R | GTCTCGTGGGCTCGGGGCTGGGAGGAACAAGAGTT | 59.5 | 0.00 |
| mm4__chr7 116.67 Mbp_chr7_116672397_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr7 116.67 Mbp_chr7_116672397_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr15 30.22 Mbp_chr15_30223283_F | TCGTCGGCAGCGTCCCAAGGTGTAGTTCTTGTGCC | 59.1 | 0.00 |
| mm4__chr15 30.22 Mbp_chr15_30223283_R | GTCTCGTGGGCTCGGGGCGCTGCATGAAGCTGG | 62.1 | 0.00 |
| mm4__chr6 26.12 Mbp_chr6_26123660_F | TCGTCGGCAGCGTCGTCTGGATCTCCCTGGAGGT | 60.0 | 0.00 |
| mm4__chr6 26.12 Mbp_chr6_26123660_R | GTCTCGTGGGCTCGGACTGGCATCTCTTCCAAGGC | 60.0 | 0.00 |
| mm4__chr3 114.10 Mbp_chr3_114103385_F | TCGTCGGCAGCGTCGTCTGGATCTCCCTGGAGGT | 60.0 | 0.00 |
| mm4__chr3 114.10 Mbp_chr3_114103385_R | GTCTCGTGGGCTCGGTGACACCGGCATCTCCTCTA | 60.0 | 0.00 |
| mm4__chr2 45.01 Mbp_chr2_45009203_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr2 45.01 Mbp_chr2_45009203_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr14 100.21 Mbp_chr14_100214630_F | TCGTCGGCAGCGTCGCTCTCAGGGCCCTCGTG | 62.1 | 0.00 |
| mm4__chr14 100.21 Mbp_chr14_100214630_R | GTCTCGTGGGCTCGGCGTCGAGGATCCAGCACAG | 60.2 | 0.00 |
| mm4__chr5 175.80 Mbp_chr5_175796739_F | TCGTCGGCAGCGTCCGGAGGAGTCAAGGAAGAGG | 59.1 | 0.00 |
| mm4__chr5 175.80 Mbp_chr5_175796739_R | GTCTCGTGGGCTCGGCGCGTTCTACGAACCTGTTG | 59.5 | 0.00 |
Primers underlined, off-targets in bold.
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529572 |
CACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGAGCCGTTGTCGACGA CGAGCGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC |
| mm1__chr2 131.63 Mbp_chr2_131626200 |
GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCAGGCTGTCTTCCCTTC TATCGTGGGGCGCCCCAGGCACCAGGGCATGATGGAGGGCATGCATCAGAAGGAGT |
| mm1__chr2 131.26 Mbp_chr2_131263617 |
GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC CATCGTGGG |
| mm1__chr2 130.66 Mbp_chr2_130656811 |
GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC CATCGTGGG |
| mm1__chr2 130.46 Mbp_chr2_130463858 |
CCCACGATGGAAGGGAAGACAGCCCGGGGGGCATCGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAAT GACGAGCAC |
| mm1__chr2 130.08 Mbp_chr2_130075286 |
CCCACGATGGAAGGGAAGACAGCCCGGGGGGCATCGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAAT GACGAGCAC |
| mm3__chr22 15.72 Mbp_chr22_15721548 |
GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC CATCGTGGGGTGCCCCAGGCACCAGAACATGAT |
| mm3__chr14 19.40 Mbp_chr14_19402823 |
ATCATGTTCTGGTGCCTGGGGCACCCCACGATGGAAGGGAAGACAGCCCGGGGGGCATCGTCACCTGCAAAGCCGGCCTT GCACATGCCAGAGCCGTTGTCAATGACGAGCAC |
| mm3__chr14 19.00 Mbp_chr14_18998929 |
GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC CATCGTGGGGTGCCCCAGGCACCAGAACATGAT |
| mm2__chr19 9.52 Mbp_chr19_9518211 | AGTTAACAATGGCTCCGGCATGTGCAAGGTCAACTTCGTGGGCGACAATGCCCCCCGGGCCATCTTCCCCTCCATCGTG |
| mm3__chr2 73.90 Mbp_chr2_73901376 |
GTGTGAAGAGGAGACCACCGCGCTCGTGTGTGACAATGGCTCTGGCCTGTGCAAGGCAGGCTTCGCAGGAGATGATGCCC CCCGGGCTGTCTTCCCCTCCATTGTGGG |
| mm4__chr6 131.81 Mbp_chr6_131808388 |
GCACCGGAGAGAGGCATGGTCCGGGAGTGCTTCTTCGCCCGCGCGCTCTCCTCCGCAGCCTTTGCAAGTGCCCTACACAG CCGCCCCTGCCAGGTACCTTGCCAGGGCAGGATGGTTCACTCTC |
| mm4__chr10 135.21 Kbp_chr10_135208 |
GCCAGGGGAAGACGCGAGTAAGTGCGGGCGGCGGTGCCCTCCCGGCCCTCACCCCGCACGCGGCGACCCGGCCGCCTAGC CTCCCTCGCTCAGGCGCGACCGCGGCGTTCTCTGAGGTGGAGTA |
| mm4__chr2 131.04 Mbp_chr2_131040038 |
TGCAGCTGAGGGTGAATCAGGACGAGCCCGCGGATGACGACGCCCCTCTGGCCGGGAACAGCGGAGCGGAGGACGGCGGG GCGGAGGCGCAGAGCAGCAAGGACCAGAT |
| mm4__chr16 1.26 Mbp_chr16_1262928 |
GCCCAGTGGTAGCTGTAGACCCTCAGGCTGACCTGCCACGGCCACTTCCCCGGGGGGGCATTGTGGCCCCCCACAATGCC CACCAGGTCATTCTCTGGGACGG |
| mm4__chr5 36.88 Mbp_chr5_36876922 | CGCCGACAGGAGAATTGGTTCCCGGGCCCGCGGCGATGCCCCCCCGGTAGCTCGGGCCCGTGGTCGGGTGTTTGTGAGT |
| mm4__chr17 4.74 Mbp_chr17_4739931 |
GGCCCGGTGGAGAGAGTCGCCGCCAGCCCCGGCCGCGCGCACCTGCGGGGCAGCCACCCGCGGACGCACCGAGCCCGGGG GGCGTGGCCGCCCGCTGAGCCGGCGAGGGCTCGGGCAGCCCTGACGTCACAACC |
| mm4__chr2 241.87 Mbp_chr2_241872904 |
AAGAGAAGGAAGGTGGCCTGGTCACCGCGGGTCACGACGCCCCTCTGGAGGCCAATGCCGAGGGCCCCATCACGGTTAGT GAGGGCTGCATCA |
| mm4__chr9 105.04 Mbp_chr9_105044568 |
GCCAGTCCTAGCCTTGAGTGTGAAGGGTCACCAGTGGGGCATCATCCCCTGCGTTCTGGAGTGTGCTGAATGCCAACTCC CATTACATGGGGACCAAAATGAGCTCAGGAGCATTGGTATCAAGCCAGACTGCCAGTC |
| mm4__chr4 1.45 Mbp_chr4_1449103 |
CATCACTGCAACGCCTCAACCCGTCTGCTCGGTGCGTGTGGGTCAGGTGCCCAGAACGCGGGTCCCCAGGAGCCCCAGCC AGGGGGGCTTGGTAGCCCGTGGCGCCTGCTGCTGGGGCGTGAGGCACCATCCTTAG |
| mm4__chr5 57.48 Mbp_chr5_57482619 |
ATCTCCCACGTAGCAGTCCTTCTGGCCCATGCCTACCATAACGCCCTGGTGTCGAGGACGCCCTATCATGGAGGGGAACA CAGCCCGGGGGGCATCGTCACCACCAAAGCCTGCCTTGCACATCCCTGACCCAT |
| mm4__chr13 21.02 Mbp_chr13_21023072 |
CTAGTCCAGCCCAGCAATCCCACTGCATGCACCTGCAGAGCCCCAGGGGGGCAGTGTCGCTCGAGCCGGGGACTCCTTTC CAAAG |
| mm4__chr17 76.14 Mbp_chr17_76140214 |
CTGAACCTGGCACCTGGTGGTAGCCGTGGGGGTCGGTGCCCCCCGGGGGCAGGGCGGGGGCCTTGGCGTTGAGAATGGAG AATTCCTGCAGCCACT |
| mm4__chr11 1.80 Mbp_chr11_1804010 |
CGCCACCCACATAGAAGTCCTTCTGACCCATGCCCACCGTCATGCCCTGGTGTCTGGGGCACCCCACGATGGAGGGGAAG ACGGCCCAGGGGGCATCGTCGTCGGTGAAGCTGGCCTTGCACATGCCAGAGGTGTT |
| mm4__chr1 12.58 Mbp_chr1_12584341 |
CCCCAGCACTGTTGACCTAGCTGGAAGACCACCCTGGAGAGGAAGTGGAGCAGGGCTGAGGGAGAAGGAATGAAGGCTGT CTCCCACTGTCTTCCGTGGGCCAGGAAGCCCCCCAGGCAGGGCCCTAACACTTGGCTGTT |
| mm4__chr3 19.15 Mbp_chr3_19148384 |
CCACTGCGGGCTCTTTCCGGGCGGGCGACGTGGGGGGCGAGGCACCGCGCGGCGGCGCCTGGCGGGCACTGTTGCCCGCG GAGGGACGGTGGGGGGGTTTCAGCAGCTGGC |
| mm4__chr17 82.75 Mbp_chr17_82752592 |
TTCCTAGGCGGGAGAGGGCCACCCCGGCGCGGCGTTAGGTGCACGGGGCAGACGCGCGCCTCCCTTGGGGGGACCGTGGC CCGCGGAGCGCCTGCTCTCGCACGGGGTGAAGCCTCT |
| mm4__chr1 22.54 Mbp_chr1_22537708 |
TACTGAGCATGCTGTCTGGGCACAGCCACTGCTCCCAGAGAGTGGGCAGAGGGCCAGGCACGCCACTCCTGGGGGGCAGC CTCGCCTGTGTGTTTGCAGCTTTCTCACAACT |
| mm4__chr1 226.08 Mbp_chr1_226083817 |
CAGCTCCTTTCCGTCCCGGGGCCTGGGGGGCACCGTCCCACGGGTGAGGGCCGCCTCCAGCGCCTGGCCCAGCCATGCGA ACGCCTGGAGCCCGCGAGCTGCAGCTGCGGCCCTGAAGGTGCCGCATATCCT |
| mm4__chr4 8.43 Mbp_chr4_8428550 |
CCCTGACGTCATCGAACCGCGCCACCCTCGTTGCCCTGGAAACGCGGACGCCGCTGCCACCCGGGCGGGCTGGAAGCCAC GAAGCTTCGCCAGGCGCTCCCGGGCACTCTTGCCTTCTCTGCTTTTAGGCA |
| mm4__chr20 63.69 Mbp_chr20_63690635 | CCTCCCCTACAGGCAGAGAACGCCCCAGGCAAGGATGCCCCCCGAGGCTGAGACTCCCCCCAATAGCAGGGAGGACAC |
| mm4__chr6 32.85 Mbp_chr6_32853586 |
GTAGCAGTACTGTCCCCAGCCATGCGAGAGAAGCTCCGGGGAGGCAGCGGCACCCGCGGGGAGCGGGACACCTAGAGCTA GCCA |
| mm4__chr12 130.42 Mbp_chr12_130424629 |
CTGTGCTTCACCGGGAACCAGGAGCCCCGAGGGGCCTCGTCGCCCCTGTAGGGCCTGCCGGGCCTGGGCTCTCTGGCCAG CCCCGTCCTGGCCCTGGGGGACGGCCCTGCACCCTGCTTGTGTAGCAGCCCACAGC |
| mm4__chr4 74.67 Mbp_chr4_74671272 | GCCTATTTACAGACACGGATGGGTAAGAAAGGGCAACGATGCCCCCCAGAGGCTAAAGGCAGGAGCTTGGA |
| mm3__chr5 77.78 Mbp_chr5_77784948 |
ATATCACCGCGCTCGTCATTGACAACGGCTCCGGCATGTGCAAGGCCAGCTTCACGGGCGACAATGCCGCCCGGGCAGTC TTCCCCTCCATCGTT |
| mm4__chr6 22.57 Mbp_chr6_22570261 |
CTCAGCCAGAGGAGGAGGAGCTCCGGGAGGAAGAAGTCGCGGACGAGGAGGCCTCCCAGGAGTGGCATGCCGAGGCACCG GGCGGCGGAGATCGCGACAGCCTGTAGTTAC |
| mm3__chr1 237.93 Mbp_chr1_237926892 |
TGACAATGGCTCCAGCATGTGCAAAGCTGGCTTTGCTGGGGACGATGCCCCCCAAGCTGTGTTTCCCTCCATCATTGGGC ACCCCCGGCACAAGAGCATGATGGTGGGCATGGGCCAGAAGGACTCCTACGTG |
| mm4__chr22 37.05 Mbp_chr22_37051773 |
GAGGAGGATGCAGACGCCGCGGCCGGCGATGAGGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNAAGTGGGTGCGGCTCAACGTGGGGGGCACGGTGTTCCTGACCA |
| mm4__chr9 134.81 Mbp_chr9_134810346 |
AGGTAAGTATTGGCACGGGGGCGCGCGGCAGCCCCCAGGTCCCGGGGGGCCTCGTCGCAGGCTGTAGGCCGTGTGCCATG CACGTTCTCTTCCAACGTTGCTGATGACTAGCGGGACATGCTG |
| mm4__chrX 48.83 Mbp_chrX_48826477 |
CTGCGCATGTCCACTCCCCCAACCGCCGCCGGGGTGCCCCGGGGAGCAGCGTCGCCCCCCGCCAGGCCTGTCAGAGCGTC |
| mm4__chr7 6.85 Mbp_chr7_6854999 |
TAGGTCAGGGCCTCTCACCACCGCGCCCAGAGGCCGGGCCAGCCGGGCGTGCATCCCAGGGGCGCTCCGTCCCCCGCGGC CTGGGACTCTCCAGTCAGGACAGTACAAAATCT |
| mm4__chr11 71.61 Mbp_chr11_71608060 |
TGTACTGTCCTGACTGGGGAGTCCCAGGCCGCGGGGGACGGAGCGCCCCTGGGATGCACGCCCGGCTGGCCCGGCCTCTG GGCGCGGTGGTGACAGGCCCTGACCTA |
| mm4__chr11 67.71 Mbp_chr11_67711211 |
CCTAGGTCAGGGCCTGTCACCACCGCGCCCAGAGGCCGGGCCAGCCGGGCGTGCATCCCAGGGGCGCTCCGTCCCCCGCG GCCTGGGACTCCCCAGTAAGGACAG |
| mm4__chr17 75.63 Mbp_chr17_75634103 |
CTCCAGGACGACCAACAACAAAAAAGCCAGGCAGAGACAGCAGCTGGCTGTCAGCAGAGGAGCTGGGCTGAGGCGCCCAG GGGAGCAGCGGCGCCCACGAAGGAAGTACGAGGACAGCACGTG |
| mm3__chr3 175.98 Mbp_chr3_175978494 |
GAGTCCTTCTGACCCATGCCCACCATCACACCTTGGTGCCTGGGGCGCCCCACGATGGATGGGAAGACGGCCCCGGAGGC ATCGTCGCCTGCGAAGCTGGTCTTGCACATACAGGAGCCTTTGC |
| mm4__chr22 19.45 Mbp_chr22_19447974 |
GGGGTCGCAGGGTAAATCCGGGGCGAGACCTGGCGCGAAGCCTACGCGACCACCGCGACGACAGCGCCATTCACCCCGGG CCAGGCGGGCATCCTGGGCCGCGCAGCTACCTTACGTCAGCCGCCTCTCAAATCA |
| mm4__chr22 20.36 Mbp_chr22_20359162 |
CTAACGAGGTCGCTGCCCATGGCATCGCGAACGAGGATGCCGCCCAGGGCATCGCGAACGAGGACGCCGCCCAGGGCATC GCACACGAGGACGCCGTCCAGGGCATCGCTAACGAGGAC |
| mm4__chr6 131.63 Mbp_chr6_131628374 |
GTTCCGTCTGTGGAGAAGGGCGGCCCGCAAGCCGGACGAGAGCGCCCCCAAACGGAACCTTGAGCCCAAGGACCCCGGAG GCATCGTCGACCGCAGGCCGCCACCTCCCGGCTGGAGAAGGAGTTGCTT |
| mm4__chrY 345.05 Kbp_chrY_345052 |
GATGACTCTTAGAGGGGCCGGGAGGCAGTGGCTGCTGTGAGGACACCTGGGCGGCACCGGCTCCCGCGGAGGTATATGAA CGACCGCTAAGCCTGGGGCTCCACCTAGCCCGGGATTGGATGGAAACT |
| mm3__chr22 41.08 Mbp_chr22_41075154 |
GGAGTCCTTCTGACCCATGCCCACCATCATGACCTGGTGCCTGGTGCACCCCACGATGGAAGGGAAGATGGCCCAGGGGA CATCGTCACCCGCGAAGCCGGCCTTGCACATGCCAGAGCTGTTGTCAACA |
| mm4__chr6 100.88 Mbp_chr6_100881496 |
CTGGGTTCGGAGGAGCAGAGGCGGAAGGTCCCGGCGGCGGCGAGGATGCGCCCCAGGGCGGGAGCGGGACGCTCCACTTT ACCCTGGCTGCCCGCAACGGGCCAGGCCTGCCGTCTCTACCTCTCT |
| mm4__chr11 66.70 Mbp_chr11_66701055 |
TTGCCTCTCGAGTCAGTTCGCCCACCCGGGCGGCGATGGCCTCCAGGTCCCGCTCCGTGCCGGCCAGCTTGCGCTGCAGG GCCAGCACCCCAGCCAGATCGTTGCCTAGGCCCTGGGTGGACTCGATGACTT |
| mm4__chr1 227.45 Mbp_chr1_227447817 |
CAGTTCCAAAAGGCTGGGGAAAGAGCTGTTTTCTGGGAATTTGGAATGTGGGAAAAATTTCCCCAGGGAGTCAAAGTCGC CCGCGGCAGGAAGCGGGGTTCCGGCCACTGGACGGCTGGTTTTCCTGCTCTGCCACCTC |
| mm4__chr16 75.15 Mbp_chr16_75148974 |
TCTTTGTCAGGAGATGCGCCTCAGACGGGCGGCGAAGGGCTCGGGCCGCGCGGGAGCGAGTTCTGGAGCCCCGGCGGGCG CGCGCGCGCGGGACGAGGCCCCGCCGGGGCGTGGTGGGGGCGTGGCCTGACACACCTG |
| mm4__chr3 14.51 Mbp_chr3_14514349 |
ACTGTCAGGCTCGATGAAGCACACGAGGGGTGGGGAGGACAGGGTCTCGGTGGCGAAGATGCCGCCCTGGAGCTGGAGGC CAAAGCCGCTGAGGGGGTCTCCACAGAGCACGACCTCCGTGGTCTCCGTGTGCACAATCT |
| mm4__chr1 185.32 Mbp_chr1_185316570 |
TTCCTGACAGCAAGAACGGGGAGTCCCGCGCCCTCCTTCCGGCGGGTCATCCCCGCCCGCGGCGCCCTAACCCAGGCCCC CCAAAACCCTTCGAACAAAAAACTCTTGTTCCTCCCAGCC |
| mm4__chr15 30.22 Mbp_chr15_30223283 |
CCAAGGTGTAGTTCTTGTGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCAGCTTCATGCAGCGCC |
| mm4__chr6 26.12 Mbp_chr6_26123660 |
GTCTGGATCTCCCTGGAGGTGATGGTCGAGCGCTTGTTGTAATGCGCCAGGCGGGAAGCCTCGCCCGCGATGCGCTCAAA TATGTCGTTAACGAAAGAATTCATGATGCCCATGGCCTTGGAAGAGATGCCAGT |
| mm4__chr3 114.10 Mbp_chr3_114103385 |
GTCTGGATCTCCCTGGAGGTGATGGTCGAGCGCTTGTTGTAATGCGCCAGGCGGGAAGCCTCGCCCGCGACGTGCTCAAA GATGTCGTTGACGAAGGAGTTCATGATCCCCATGGCCTTAGAGGAGATGCCGGTGTCA |
| mm4__chr14 100.21 Mbp_chr14_100214630 |
GCTCTCAGGGCCCTCGTGCCCCGCCCCGCCCTCCTCCCCGGGGCCCCTCGTCCCCCGCGCCCGCCTAGCCCCGCCCCGGC TGTGCTGGATCCTCGACG |
| mm4__chr5 175.80 Mbp_chr5_175796739 |
CGGAGGAGTCAAGGAAGAGGGGGAGGGAGAAACGCGCCAGAACCTCGGCCCGGGCGCCCTCGTCGGCCGCGGAGGAGCTG CAGCCTCCAACAGGTTCGTAGAACGCG |