CRISPOR

Micro-homology scoring of potential deletions

Guide: GAAGCCGGCCTTGCACATGC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATC`	(Wild-type sequence)
315.0	`CGGGGGGCATCGTCGCCCGCGAAGCCG---------------------TTGTCGACGACGAGCGCGGCGATATCATCATC`	`21 bp deleted → no frameshift`
296.4	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC------CGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`6 bp deleted → no frameshift`
283.2	`CGGGGGGCATCGTCGCCCGCGAAGCCGG---------------AGCCGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`15 bp deleted → no frameshift`
256.2	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCC-----------------GTTGTCGACGACGAGCGCGGCGATATCATCATC`	`17 bp deleted → frameshift`
219.6	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGC------------CGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`12 bp deleted → no frameshift`
164.7	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATG------------TCGACGACGAGCGCGGCGATATCATCATC`	`12 bp deleted → no frameshift`
162.8	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTG------------------TCGACGACGAGCGCGGCGATATCATCATC`	`18 bp deleted → no frameshift`
141.0	`CGGGGGGCATCGTCGCCCG-----------------------------TTGTCGACGACGAGCGCGGCGATATCATCATC`	`29 bp deleted → frameshift`
140.0	`CGGGGGGCATCGTCGCCCGCG---------------------GAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`21 bp deleted → no frameshift`
133.8	`CGGGGGGCATCGTCGCC------------------------------GTTGTCGACGACGAGCGCGGCGATATCATCATC`	`30 bp deleted → no frameshift`
126.8	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC-----------------------GCGGCGATATCATCATC`	`23 bp deleted → frameshift`
126.8	`CGGGGGGCATCGTCGCCCG-----------------------GAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`23 bp deleted → frameshift`
114.8	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC-------------------------GGCGATATCATCATC`	`25 bp deleted → frameshift`
113.6	`CGGGGGGCATCGTCGCCCGCGAAGCCGGC---------------------------------------GATATCATCATC`	`39 bp deleted → no frameshift`
110.4	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCAC--------------------GACGAGCGCGGCGATATCATCATC`	`20 bp deleted → frameshift`
109.2	`CGGGGGGCATCGTCGCCCGC--------------------------CGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`26 bp deleted → frameshift`
103.6	`CGGGGGGCATCGTCGCCCGCGAAGCCG---------------------------ACGACGAGCGCGGCGATATCATCATC`	`27 bp deleted → no frameshift`
103.6	`CGGGGGGCATCGTCGCCCGCG---------------------------TTGTCGACGACGAGCGCGGCGATATCATCATC`	`27 bp deleted → no frameshift`
103.6	`CGGGGGGCATCGTCG---------------------------GAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`27 bp deleted → no frameshift`
99.9	`CGGGGGGCATCGTCGCCCGCGA----------------------GCCGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`22 bp deleted → frameshift`
99.4	`CGGGGGGCATCGTCG---------------------------------------ACGACGAGCGCGGCGATATCATCATC`	`39 bp deleted → no frameshift`
98.8	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC----------------------------GATATCATCATC`	`28 bp deleted → frameshift`
96.0	`CGGGGGGCATCGTCGCCCGCGA---------------------------------CGACGAGCGCGGCGATATCATCATC`	`33 bp deleted → no frameshift`
95.1	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCAC-----------------------GAGCGCGGCGATATCATCATC`	`23 bp deleted → frameshift`
94.0	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGC-----------------------------GCGGCGATATCATCATC`	`29 bp deleted → frameshift`
89.2	`CGGGGGGCATCGTCGCCCGCGAAGCCG------------------------------ACGAGCGCGGCGATATCATCATC`	`30 bp deleted → no frameshift`
89.2	`CGGGGGGCATCG------------------------------GAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`30 bp deleted → no frameshift`
84.8	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGC-------------------------------GGCGATATCATCATC`	`31 bp deleted → frameshift`
84.0	`CGGGGGGCATCGTCGCCCGCG---------------------------------------------GCGATATCATCATC`	`45 bp deleted → no frameshift`
82.5	`CGGGGGGCATCGTCGCCCGCGA------------------------------------CGAGCGCGGCGATATCATCATC`	`36 bp deleted → no frameshift`
82.5	`CGGGGGGCATCGT------------------------------------TGTCGACGACGAGCGCGGCGATATCATCATC`	`36 bp deleted → no frameshift`
81.0	`CGG----------------------------------------AGCCGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`40 bp deleted → frameshift`
76.8	`CGGGGGGCATCGTCGCCCGCGAAGCCG---------------------------------AGCGCGGCGATATCATCATC`	`33 bp deleted → no frameshift`
76.8	`CGGGGGGCATCGTCG---------------------------------TTGTCGACGACGAGCGCGGCGATATCATCATC`	`33 bp deleted → no frameshift`
75.0	`CGGGGGGCATCGTCGCCCGCGAAGC--------------------------------------GCGGCGATATCATCATC`	`38 bp deleted → frameshift`
73.2	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGC----------------------------------GATATCATCATC`	`34 bp deleted → frameshift`
73.2	`CGGGGGGCATCGTCGCCCGCGAAGCCGGC----------------------------------GCGGCGATATCATCATC`	`34 bp deleted → frameshift`
71.0	`CGGGGGGCATCGTCGCCCGCGA---------------------------------------GCGCGGCGATATCATCATC`	`39 bp deleted → no frameshift`
69.6	`CGGGGGGCATCGTCGCCCGCG-------------------------------------------CGGCGATATCATCATC`	`43 bp deleted → frameshift`
69.6	`CGGGGGGCATCGTCGCCCG-----------------------------------ACGACGAGCGCGGCGATATCATCATC`	`35 bp deleted → frameshift`
66.0	`CGGGGGGCATCGTCGCCCGCGAAGCCGGC------------------------------------GGCGATATCATCATC`	`36 bp deleted → no frameshift`
66.0	`CGGGGGG------------------------------------AGCCGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`36 bp deleted → no frameshift`
63.7	`CGGGGGGCATCGTCGCCCGCGA------------------------------------------------TATCATCATC`	`48 bp deleted → no frameshift`
62.8	`CGGGGGGCATCGTCGCCCGCGAAGCCG-------------------------------------CGGCGATATCATCATC`	`37 bp deleted → frameshift`
62.8	`CGGGGG-------------------------------------AGCCGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`37 bp deleted → frameshift`
61.0	`CGGGGGGCATCG------------------------------------------ACGACGAGCGCGGCGATATCATCATC`	`42 bp deleted → no frameshift`
60.0	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACAT--------------------------------------CATC`	`38 bp deleted → frameshift`
60.0	`CGGGGGGCATCGTCGCCCG--------------------------------------ACGAGCGCGGCGATATCATCATC`	`38 bp deleted → frameshift`
60.0	`CGGGGGGC--------------------------------------CGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`38 bp deleted → frameshift`
60.0	`CGGGG--------------------------------------AGCCGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`38 bp deleted → frameshift`
56.8	`CGGG---------------------------------------AGCCGTTGTCGACGACGAGCGCGGCGATATCATCATC`	`39 bp deleted → no frameshift`
54.0	`CGGGGGGCATCGTCGCCCGCGAAGC----------------------------------------GGCGATATCATCATC`	`40 bp deleted → frameshift`
51.6	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACAT-----------------------------------------C`	`41 bp deleted → frameshift`
51.6	`CGGGGGGCATCGTCGCCCG-----------------------------------------AGCGCGGCGATATCATCATC`	`41 bp deleted → frameshift`
51.6	`CGGGGGGCATCGTCGC-------------------------------------------------GGCGATATCATCATC`	`49 bp deleted → frameshift`
48.8	`CGGGGGGCATCGTCGCCCGCGAAGCCG------------------------------------------ATATCATCATC`	`42 bp deleted → no frameshift`
48.8	`CGGGGGGCATCGTCG------------------------------------------ACGAGCGCGGCGATATCATCATC`	`42 bp deleted → no frameshift`
46.4	`CGGGGGGCATCGTCGCCCGCGAAGC-------------------------------------------GATATCATCATC`	`43 bp deleted → frameshift`
42.0	`CGGGGGGCATCGTCG---------------------------------------------AGCGCGGCGATATCATCATC`	`45 bp deleted → no frameshift`
42.0	`CGGGGGGCATCG---------------------------------------------ACGAGCGCGGCGATATCATCATC`	`45 bp deleted → no frameshift`
40.5	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA----------------------------------------TCATC`	`40 bp deleted → frameshift`
40.0	`CG----------------------------------------------TTGTCGACGACGAGCGCGGCGATATCATCATC`	`46 bp deleted → frameshift`
38.4	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACAT---------------------------------ATCATCATC`	`33 bp deleted → no frameshift`
38.0	`CGGGGGGCATCGTCGCCCG-----------------------------------------------GCGATATCATCATC`	`47 bp deleted → frameshift`
38.0	`CGGGGGGCATCGTCGC-----------------------------------------------GCGGCGATATCATCATC`	`47 bp deleted → frameshift`
36.4	`CGGGGGGCATCG------------------------------------------------AGCGCGGCGATATCATCATC`	`48 bp deleted → no frameshift`
34.8	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA-------------------------------------------TC`	`43 bp deleted → frameshift`
34.8	`CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACAT-----------------------------------CATCATC`	`35 bp deleted → frameshift`
32.8	`CGGGGGGCATCGTCGCCCG--------------------------------------------------ATATCATCATC`	`50 bp deleted → frameshift`
31.2	`CGGGGGGCATCGTCG---------------------------------------------------GCGATATCATCATC`	`51 bp deleted → no frameshift`
30.0	`CGGGGGGC------------------------------------------------------------GATATCATCATC`	`60 bp deleted → no frameshift`
29.6	`CGGGGGGCATCGTCGC----------------------------------------------------GATATCATCATC`	`52 bp deleted → frameshift`
29.6	`CGGGGGGCATCG----------------------------------------------------CGGCGATATCATCATC`	`52 bp deleted → frameshift`
29.6	`CG----------------------------------------------------ACGACGAGCGCGGCGATATCATCATC`	`52 bp deleted → frameshift`
26.8	`CGGGGGGCATCGTCG------------------------------------------------------ATATCATCATC`	`54 bp deleted → no frameshift`
26.8	`CGGGGGGCATCG------------------------------------------------------GCGATATCATCATC`	`54 bp deleted → no frameshift`
25.6	`CGGGGGGC-------------------------------------------------------GCGGCGATATCATCATC`	`55 bp deleted → frameshift`
25.6	`CG-------------------------------------------------------ACGAGCGCGGCGATATCATCATC`	`55 bp deleted → frameshift`
24.6	`CGG----------------------------------------------------------------CGATATCATCATC`	`64 bp deleted → frameshift`
23.2	`CGGGGGGCATCG---------------------------------------------------------ATATCATCATC`	`57 bp deleted → no frameshift`
23.2	`CGGGGGGC---------------------------------------------------------GGCGATATCATCATC`	`57 bp deleted → no frameshift`
22.2	`CGGGGGGCATC------------------------------------------------------------------ATC`	`66 bp deleted → no frameshift`
22.0	`CG----------------------------------------------------------AGCGCGGCGATATCATCATC`	`58 bp deleted → frameshift`
19.2	`CGGGGGGCATC---------------------------------------------------------------------`	`69 bp deleted → no frameshift`
18.8	`CGGGGG-------------------------------------------------------------CGATATCATCATC`	`61 bp deleted → frameshift`
18.0	`CGGGG--------------------------------------------------------------CGATATCATCATC`	`62 bp deleted → frameshift`
18.0	`CG--------------------------------------------------------------CGGCGATATCATCATC`	`62 bp deleted → frameshift`
17.2	`CGGGGGGCATC---------------------------------------------------------------ATCATC`	`63 bp deleted → no frameshift`
17.2	`CGGG---------------------------------------------------------------CGATATCATCATC`	`63 bp deleted → no frameshift`
15.0	`CGGGGGGCATCGTC------------------------------------------------------------ATCATC`	`60 bp deleted → no frameshift`
14.0	`CG-------------------------------------------------------------------ATATCATCATC`	`67 bp deleted → frameshift`
12.9	`CGGGGGGCATCGTC---------------------------------------------------------------ATC`	`63 bp deleted → no frameshift`
11.1	`CGGGGGGCATCGTC------------------------------------------------------------------`	`66 bp deleted → no frameshift`
9.4	`CGGGGGGCAT-------------------------------------------------------------ATCATCATC`	`61 bp deleted → frameshift`

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