CRISPOR

Micro-homology scoring of potential deletions

Guide: GCGCCGGCGCGCGCCCAGAT TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	(Wild-type sequence)
240.8	`AGTCCTTAGGCCGCCAGGGGG------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`24 bp deleted → no frameshift`
229.6	`AGTCCTTAGGCCGCCAGGGG-------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`25 bp deleted → frameshift`
211.5	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGA-------CAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`7 bp deleted → frameshift`
202.0	`AGTCCTTAGGCCGCCAGGGGGCGCCGG--------------------------------GCCGGCCGCGTTATTACCATA`	`32 bp deleted → frameshift`
199.8	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCC----------------------GGGCCGGCCGCGTTATTACCATA`	`22 bp deleted → frameshift`
190.2	`AGTCCTTAGGCCGCCAGGGGG-----------------------GACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`23 bp deleted → frameshift`
188.4	`AGTCCTTAGGCCGCCAGGGGGCGCCGGC-------------------------------------CGCGTTATTACCATA`	`37 bp deleted → frameshift`
179.6	`AGTCCTTAGGCCGCCAGGGGGCGCCGG----------------GGACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`16 bp deleted → frameshift`
173.1	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCA-----------AAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`11 bp deleted → frameshift`
170.8	`AGTCCTTAGGCCGCCAGGGGGCGCCGG-----------------GACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`17 bp deleted → frameshift`
163.8	`AGTCCTTAGGCCGCCAGGG--------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`26 bp deleted → frameshift`
162.8	`AGTCCTTAGGCCGCCAGGGGGCGCCGG------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`18 bp deleted → no frameshift`
156.6	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAG-------------GAAGCCGGGCCGGCCGCGTTATTACCATA`	`13 bp deleted → frameshift`
155.4	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCC---------------------------GGCCGCGTTATTACCATA`	`27 bp deleted → no frameshift`
149.1	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGA--------------AGCCGGGCCGGCCGCGTTATTACCATA`	`14 bp deleted → frameshift`
140.0	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCC---------------------GGGCCGGCCGCGTTATTACCATA`	`21 bp deleted → no frameshift`
133.2	`AGTCCTTAGGCCGCCAGGGGG----------------------GGACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`22 bp deleted → frameshift`
132.0	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCG------------------------------------TTATTACCATA`	`36 bp deleted → no frameshift`
128.1	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAG-----------------CCGGGCCGGCCGCGTTATTACCATA`	`17 bp deleted → frameshift`
127.2	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCC-------------------------------GCGTTATTACCATA`	`31 bp deleted → frameshift`
120.4	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGC------------------------CGGGCCGGCCGCGTTATTACCATA`	`24 bp deleted → no frameshift`
120.0	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCG--------------------------------------TTATTACCATA`	`38 bp deleted → frameshift`
115.2	`AGTCCTTAGGCCGCCAGGGGGCGCCGGC---------------------------------CGGCCGCGTTATTACCATA`	`33 bp deleted → no frameshift`
114.8	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCG-------------------------GGCCGGCCGCGTTATTACCATA`	`25 bp deleted → frameshift`
114.8	`AGTCCTTAGGCCGCCAGGGGGCGCCGG-------------------------AAGCCGGGCCGGCCGCGTTATTACCATA`	`25 bp deleted → frameshift`
113.6	`AGTCCTTAGGCCGCCAGGGGGC---------------------------------------CGGCCGCGTTATTACCATA`	`39 bp deleted → no frameshift`
109.8	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGC----------------------------------GTTATTACCATA`	`34 bp deleted → frameshift`
109.2	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCC--------------------------GGCCGCGTTATTACCATA`	`26 bp deleted → frameshift`
109.2	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGC--------------------------CGGGCCGGCCGCGTTATTACCATA`	`26 bp deleted → frameshift`
103.6	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCG---------------------------GGCCGGCCGCGTTATTACCATA`	`27 bp deleted → no frameshift`
103.6	`AGTCCTTAGGCCGCCAGG---------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`27 bp deleted → no frameshift`
103.2	`AGTCCTTAGGCCGCCAGGGGGCGCCG-----------------------------------------CGTTATTACCATA`	`41 bp deleted → frameshift`
98.8	`AGTCCTTAGGCCGCCAGGGGGCGCCGGC----------------------------CGGGCCGGCCGCGTTATTACCATA`	`28 bp deleted → frameshift`
94.0	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGC-----------------------------CGGCCGCGTTATTACCATA`	`29 bp deleted → frameshift`
94.0	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCG-----------------------------GGCCGGCCGCGTTATTACCATA`	`29 bp deleted → frameshift`
91.5	`AGTCCTTAGGCCGCCAGG----------------------------------AAGCCGGGCCGGCCGCGTTATTACCATA`	`34 bp deleted → frameshift`
89.2	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCC------------------------------GCGTTATTACCATA`	`30 bp deleted → no frameshift`
89.2	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCG------------------------------GCCGCGTTATTACCATA`	`30 bp deleted → no frameshift`
84.8	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGC-------------------------------CGGCCGCGTTATTACCATA`	`31 bp deleted → frameshift`
84.8	`AGTCCTTAGGCCGCCAGGGGG-------------------------------AAGCCGGGCCGGCCGCGTTATTACCATA`	`31 bp deleted → frameshift`
84.0	`AGTCCTTAGGCCG---------------------------------------------GGCCGGCCGCGTTATTACCATA`	`45 bp deleted → no frameshift`
82.0	`AGTCCTTAGGCCG--------------------------------------------------GCCGCGTTATTACCATA`	`50 bp deleted → frameshift`
81.0	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCG----------------------------------------TTATTACCATA`	`40 bp deleted → frameshift`
81.0	`AGTCCTTAGGCCGCCAGGGG----------------------------------------CCGGCCGCGTTATTACCATA`	`40 bp deleted → frameshift`
80.8	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCG--------------------------------GCCGCGTTATTACCATA`	`32 bp deleted → frameshift`
80.8	`AGTCCTTAGGCCGCCAGGGG--------------------------------AAGCCGGGCCGGCCGCGTTATTACCATA`	`32 bp deleted → frameshift`
80.4	`AGTCCTTAGGCCGC------------------------------------------------------GTTATTACCATA`	`54 bp deleted → no frameshift`
77.4	`AGTCCTTAGGCCGCCAGGG-----------------------------------------CCGGCCGCGTTATTACCATA`	`41 bp deleted → frameshift`
76.8	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGC---------------------------------CGCGTTATTACCATA`	`33 bp deleted → no frameshift`
76.8	`AGTCCTTAGGCCGCCAGGG---------------------------------AAGCCGGGCCGGCCGCGTTATTACCATA`	`33 bp deleted → no frameshift`
76.8	`AGTCCTTAGG---------------------------------GGACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`33 bp deleted → no frameshift`
73.2	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCG----------------------------------GCCGCGTTATTACCATA`	`34 bp deleted → frameshift`
73.2	`AGTCCTTAGGCCGCCAGGGGGC----------------------------------CGGGCCGGCCGCGTTATTACCATA`	`34 bp deleted → frameshift`
73.2	`AGTCCTTAGG----------------------------------GACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`34 bp deleted → frameshift`
73.2	`AGTCCTTAGGCCGCC------------------------------------------GGGCCGGCCGCGTTATTACCATA`	`42 bp deleted → no frameshift`
69.6	`AGTCCTTAGGCCGCCAGGGGGC-------------------------------------------CGCGTTATTACCATA`	`43 bp deleted → frameshift`
69.6	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGC-----------------------------------CGCGTTATTACCATA`	`35 bp deleted → frameshift`
69.6	`AGTCCTTAGGCCGCCAGGGGGCG-----------------------------------GGCCGGCCGCGTTATTACCATA`	`35 bp deleted → frameshift`
69.6	`AGTCCTTAGG-----------------------------------ACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`35 bp deleted → frameshift`
66.6	`AGTCCTTAGGCCGCCAGGGGGCGC--------------------------------------------GTTATTACCATA`	`44 bp deleted → frameshift`
64.5	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCA-----------------------------------------TA`	`41 bp deleted → frameshift`
61.0	`AGTCCTTAGG------------------------------------------AAGCCGGGCCGGCCGCGTTATTACCATA`	`42 bp deleted → no frameshift`
60.6	`AGTCCTTAGGCCGCCA--------------------------------AAGGAAGCCGGGCCGGCCGCGTTATTACCATA`	`32 bp deleted → frameshift`
60.0	`AGTCCTTAGGCCGCCAGGGGGCG----------------------------------------------TTATTACCATA`	`46 bp deleted → frameshift`
60.0	`AGTCCTTAGGCCGCCAGGGGG--------------------------------------GCCGGCCGCGTTATTACCATA`	`38 bp deleted → frameshift`
57.0	`AGTCCTTAGGCCGCC-----------------------------------------------GGCCGCGTTATTACCATA`	`47 bp deleted → frameshift`
54.0	`AGTCCTTAGGCCGCCAGGGGGCG----------------------------------------GCCGCGTTATTACCATA`	`40 bp deleted → frameshift`
48.8	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCC------------------------------------------ATA`	`42 bp deleted → no frameshift`
48.8	`AGTCCTTAGGCCGCCAGG------------------------------------------CCGGCCGCGTTATTACCATA`	`42 bp deleted → no frameshift`
46.8	`AGTCCTTAGGCCGCC---------------------------------------------------GCGTTATTACCATA`	`51 bp deleted → no frameshift`
46.4	`AGTCCTTAGGCCGCCAGGGGGCGCCG-------------------------------------------TTATTACCATA`	`43 bp deleted → frameshift`
45.0	`AGTCCTTAGGCCGCCAG--------------------------------------CCGGGCCGGCCGCGTTATTACCATA`	`38 bp deleted → frameshift`
44.4	`AGTCCTTAGGCCGCCAGGGG--------------------------------------------CCGCGTTATTACCATA`	`44 bp deleted → frameshift`
42.0	`AGTCCTTAGGCCGCCAGGG---------------------------------------------CCGCGTTATTACCATA`	`45 bp deleted → no frameshift`
40.0	`AGTCCTTAGGCCGCCAGG----------------------------------------------CCGCGTTATTACCATA`	`46 bp deleted → frameshift`
38.4	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGAT---------------------------------TACCATA`	`33 bp deleted → no frameshift`
34.4	`AGTCCTTAGG-------------------------------------------------GCCGGCCGCGTTATTACCATA`	`49 bp deleted → frameshift`
30.0	`AGTCCTTAG----------------------------------------------CCGGGCCGGCCGCGTTATTACCATA`	`46 bp deleted → frameshift`
29.6	`AGTCCTTAGGCCGCCAGGGGGCGCC----------------------------------------------------ATA`	`52 bp deleted → frameshift`
29.6	`AGTCC----------------------------------------------------GGGCCGGCCGCGTTATTACCATA`	`52 bp deleted → frameshift`
28.4	`AGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGAT---------------------------------------A`	`39 bp deleted → no frameshift`
25.8	`AG-------------------------------------------------GAAGCCGGGCCGGCCGCGTTATTACCATA`	`49 bp deleted → frameshift`
24.4	`AGTCCTTAGGCCG--------------------------------------------------------TTATTACCATA`	`56 bp deleted → frameshift`
23.2	`AGTCCTTAGGC---------------------------------------------------------GTTATTACCATA`	`57 bp deleted → no frameshift`
23.2	`AGTCC---------------------------------------------------------GGCCGCGTTATTACCATA`	`57 bp deleted → no frameshift`
22.5	`AGTCCTTAGGCCGCCA--------------------------------------------------------------TA`	`62 bp deleted → frameshift`
21.3	`AG-----------------------------------------------------CCGGGCCGGCCGCGTTATTACCATA`	`53 bp deleted → frameshift`
18.8	`AGTCC-------------------------------------------------------------GCGTTATTACCATA`	`61 bp deleted → frameshift`
15.6	`AGTCCTTAGGCC-----------------------------------------------------------------ATA`	`65 bp deleted → frameshift`
12.3	`AGTCCTTA----------------------------------------------------------------TTACCATA`	`64 bp deleted → frameshift`
10.8	`AGTCC------------------------------------------------------------------------ATA`	`72 bp deleted → no frameshift`
10.5	`AGTCCTTA-------------------------------------------------------------------CCATA`	`67 bp deleted → frameshift`
10.5	`AGT-------------------------------------------------------------------TATTACCATA`	`67 bp deleted → frameshift`
5.4	`AGTCCTTA------------------------------------------------------------------------`	`72 bp deleted → no frameshift`

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