CRISPOR

Micro-homology scoring of potential deletions

Guide: GGCCTAAGGACTCGGCGCGC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	(Wild-type sequence)
402.0	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGG--------AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`8 bp deleted → frameshift`
344.4	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCG---GAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`3 bp deleted → no frameshift`
311.6	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCG-----GAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`5 bp deleted → frameshift`
298.2	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGC--------------CAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`14 bp deleted → frameshift`
294.4	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCG--------------------GGGGCGACCTCGGCTCACAGCGC`	`20 bp deleted → frameshift`
248.5	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGA--------------AGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`14 bp deleted → frameshift`
244.2	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCG------------------GGGGCGACCTCGGCTCACAGCGC`	`18 bp deleted → no frameshift`
242.8	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGC----------CAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`10 bp deleted → frameshift`
219.6	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGC------------CAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`12 bp deleted → no frameshift`
218.4	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCG--------------------------ACCTCGGCTCACAGCGC`	`26 bp deleted → frameshift`
210.0	`GCGCGCCGGCGCCCCCTGGCGG---------------------AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`21 bp deleted → no frameshift`
207.2	`GCGCGCCGGCGCCCCCTGGCGGCC---------------------------AGGGCGGGGGCGACCTCGGCTCACAGCGC`	`27 bp deleted → no frameshift`
191.4	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGC-----------------------------------TCACAGCGC`	`35 bp deleted → frameshift`
190.2	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGG-----------------------GGGCGACCTCGGCTCACAGCGC`	`23 bp deleted → frameshift`
180.6	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCG------------------------ACCTCGGCTCACAGCGC`	`24 bp deleted → no frameshift`
179.6	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGC----------------GGGGGCGACCTCGGCTCACAGCGC`	`16 bp deleted → frameshift`
165.0	`GCGCGCCGGCGCCCCCTGGCGG------------------------------------GGGCGACCTCGGCTCACAGCGC`	`36 bp deleted → no frameshift`
162.8	`GCGCGCCGGCGCCCCCTGGCGGCCTAAG------------------TGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`18 bp deleted → no frameshift`
156.1	`GCGCGCCGGCGCCCCCTGGC------------------------------CAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`30 bp deleted → no frameshift`
154.8	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGG-------------------GCGGGGGCGACCTCGGCTCACAGCGC`	`19 bp deleted → frameshift`
147.2	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGG--------------------CCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`20 bp deleted → frameshift`
143.5	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGG-------------------------GCGGGGGCGACCTCGGCTCACAGCGC`	`25 bp deleted → frameshift`
133.2	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGC----------------------GACCTCGGCTCACAGCGC`	`22 bp deleted → frameshift`
120.4	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGG------------------------GGCGACCTCGGCTCACAGCGC`	`24 bp deleted → no frameshift`
120.4	`GCGCGCCGGCGCCCCCTGG------------------------AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`24 bp deleted → no frameshift`
115.2	`GCGCGCCGGCGCCCCCTGGCGGC---------------------------------GGGGGCGACCTCGGCTCACAGCGC`	`33 bp deleted → no frameshift`
114.8	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGG-------------------------GCGACCTCGGCTCACAGCGC`	`25 bp deleted → frameshift`
109.8	`GCGCGCCGG----------------------------------AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`34 bp deleted → frameshift`
109.2	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGG--------------------------CGGGGGCGACCTCGGCTCACAGCGC`	`26 bp deleted → frameshift`
108.0	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGC----------------------------------------`	`40 bp deleted → frameshift`
97.6	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGC------------------------------------------`	`42 bp deleted → no frameshift`
97.6	`GCGCGCCGGCGCCCCCTGGCG------------------------------------------ACCTCGGCTCACAGCGC`	`42 bp deleted → no frameshift`
94.0	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCG-----------------------------ACCTCGGCTCACAGCGC`	`29 bp deleted → frameshift`
94.0	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGG-----------------------------GGGCGACCTCGGCTCACAGCGC`	`29 bp deleted → frameshift`
91.5	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGAC----------------------------------CTCGGCTCACAGCGC`	`34 bp deleted → frameshift`
90.0	`GCGCGCCGGCGCC--------------------------------------AGGGCGGGGGCGACCTCGGCTCACAGCGC`	`38 bp deleted → frameshift`
89.2	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCG------------------------------GCTCACAGCGC`	`30 bp deleted → no frameshift`
89.2	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGG------------------------------GGCGACCTCGGCTCACAGCGC`	`30 bp deleted → no frameshift`
85.2	`GCGCGCCGGCGCCCCCTGGCGGC---------------------------------------GACCTCGGCTCACAGCGC`	`39 bp deleted → no frameshift`
84.8	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGC-------------------------------TCACAGCGC`	`31 bp deleted → frameshift`
84.8	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGG-------------------------------GCGACCTCGGCTCACAGCGC`	`31 bp deleted → frameshift`
84.8	`GCGCGCCGGCG-------------------------------GAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`31 bp deleted → frameshift`
81.0	`GCGCGCCGGC----------------------------------------CAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`40 bp deleted → frameshift`
80.8	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCG--------------------------------GCTCACAGCGC`	`32 bp deleted → frameshift`
80.8	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGG--------------------------------CGACCTCGGCTCACAGCGC`	`32 bp deleted → frameshift`
80.8	`GCGCGCCGGCGCCCCCTGGCGG--------------------------------GCGGGGGCGACCTCGGCTCACAGCGC`	`32 bp deleted → frameshift`
80.0	`GCGCGCCGGCG----------------------------------------------GGGGCGACCTCGGCTCACAGCGC`	`46 bp deleted → frameshift`
76.8	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGC---------------------------------TCACAGCGC`	`33 bp deleted → no frameshift`
72.8	`GCGCGCCGGCGCCCCCTGGCGGC------------------------------------------------TCACAGCGC`	`48 bp deleted → no frameshift`
69.6	`GCGCGCCGGCGCCCCCTGG-----------------------------------GCGGGGGCGACCTCGGCTCACAGCGC`	`35 bp deleted → frameshift`
69.6	`GCGCGCCGGCGCCCCC-----------------------------------AGGGCGGGGGCGACCTCGGCTCACAGCGC`	`35 bp deleted → frameshift`
67.5	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTC----------------------------------------ACAGCGC`	`40 bp deleted → frameshift`
66.6	`GCGCGCC--------------------------------------------AGGGCGGGGGCGACCTCGGCTCACAGCGC`	`44 bp deleted → frameshift`
66.0	`GCGCGCCGGCGCCCC------------------------------------AGGGCGGGGGCGACCTCGGCTCACAGCGC`	`36 bp deleted → no frameshift`
62.8	`GCGCGCCGGCGCCCCCTGGCGG-------------------------------------GGCGACCTCGGCTCACAGCGC`	`37 bp deleted → frameshift`
62.8	`GCGCGCCGGCGCCC-------------------------------------AGGGCGGGGGCGACCTCGGCTCACAGCGC`	`37 bp deleted → frameshift`
62.8	`GCGCG-------------------------------------GAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`37 bp deleted → frameshift`
61.0	`GCGCGCCGGCGCCCCCTGGCGGCCT------------------------------------------CGGCTCACAGCGC`	`42 bp deleted → no frameshift`
60.0	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGC--------------------------------------GC`	`38 bp deleted → frameshift`
60.0	`GCGCGCCGGCGCCCCCTGGCGG--------------------------------------GCGACCTCGGCTCACAGCGC`	`38 bp deleted → frameshift`
59.2	`GCGCGCCGGCG----------------------------------------------------ACCTCGGCTCACAGCGC`	`52 bp deleted → frameshift`
56.8	`GCGCGCCGGCGCCCCCTGG---------------------------------------GGGCGACCTCGGCTCACAGCGC`	`39 bp deleted → no frameshift`
56.8	`GCG---------------------------------------GAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`39 bp deleted → no frameshift`
54.0	`GCGCGCCGGCGCCCCCTGG----------------------------------------GGCGACCTCGGCTCACAGCGC`	`40 bp deleted → frameshift`
51.6	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGG-----------------------------------------CTCACAGCGC`	`41 bp deleted → frameshift`
51.6	`GCGCGCCGGCGCCCCCTGG-----------------------------------------GCGACCTCGGCTCACAGCGC`	`41 bp deleted → frameshift`
51.6	`GCGCGCCGG-------------------------------------------------GGGCGACCTCGGCTCACAGCGC`	`49 bp deleted → frameshift`
46.8	`GCGCGCCGGCGCCCCCTGGC---------------------------------------------------TCACAGCGC`	`51 bp deleted → no frameshift`
44.4	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGC--------------------------------------------`	`44 bp deleted → frameshift`
44.4	`GCGCGCCGGCGC--------------------------------------------GGGGGCGACCTCGGCTCACAGCGC`	`44 bp deleted → frameshift`
44.4	`GCGCG----------------------------------------------------GGGGCGACCTCGGCTCACAGCGC`	`52 bp deleted → frameshift`
42.0	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCG---------------------------------------------C`	`45 bp deleted → no frameshift`
42.0	`GCGCGCCGG---------------------------------------------GCGGGGGCGACCTCGGCTCACAGCGC`	`45 bp deleted → no frameshift`
41.0	`GCGCGCCGGCGCCCCCT--------------------------------------------------CGGCTCACAGCGC`	`50 bp deleted → frameshift`
40.2	`GCG------------------------------------------------------GGGGCGACCTCGGCTCACAGCGC`	`54 bp deleted → no frameshift`
40.0	`GCGC----------------------------------------------CAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`46 bp deleted → frameshift`
37.6	`GCGCGCCGGC-------------------------------------------------------------TCACAGCGC`	`61 bp deleted → frameshift`
36.4	`GC------------------------------------------------CAGGGCGGGGGCGACCTCGGCTCACAGCGC`	`48 bp deleted → no frameshift`
33.3	`GCGCGCCGGCGCCCCCTGGCGGCCTAAGGAC--------------------------------------------AGCGC`	`44 bp deleted → frameshift`
33.0	`GCGCGCCGGCGCCCCCTGGCG----------------------------------------------------------C`	`58 bp deleted → frameshift`
33.0	`GCGCG----------------------------------------------------------ACCTCGGCTCACAGCGC`	`58 bp deleted → frameshift`
32.8	`GCGCGCCGGCGC--------------------------------------------------GACCTCGGCTCACAGCGC`	`50 bp deleted → frameshift`
32.8	`GCGCGCCGG--------------------------------------------------GGCGACCTCGGCTCACAGCGC`	`50 bp deleted → frameshift`
32.8	`GCGCGC--------------------------------------------------GGGGGCGACCTCGGCTCACAGCGC`	`50 bp deleted → frameshift`
31.2	`GCGCGCCGGCGCCCC---------------------------------------------------TCGGCTCACAGCGC`	`51 bp deleted → no frameshift`
31.2	`GCGCGCCGG---------------------------------------------------GCGACCTCGGCTCACAGCGC`	`51 bp deleted → no frameshift`
30.0	`GCG------------------------------------------------------------ACCTCGGCTCACAGCGC`	`60 bp deleted → no frameshift`
29.6	`GCGCGCCGGCGCCC----------------------------------------------------TCGGCTCACAGCGC`	`52 bp deleted → frameshift`
28.5	`GCGCGCCGGCGCCCCCTGGCGGCCT-----------------------------------------------CACAGCGC`	`47 bp deleted → frameshift`
28.4	`GCGCGCCGGCGCC-----------------------------------------------------TCGGCTCACAGCGC`	`53 bp deleted → frameshift`
26.4	`GCGCGCCGGCGC--------------------------------------------------------------------`	`68 bp deleted → frameshift`
25.8	`GCGCGCCGGCGCCCCCTGGCGGCCTAAG-------------------------------------------------CGC`	`49 bp deleted → frameshift`
25.6	`GCGCGCCGGCGCCCCCTGGCGGC-------------------------------------------------------GC`	`55 bp deleted → frameshift`
25.6	`GCGCGCCG-------------------------------------------------------ACCTCGGCTCACAGCGC`	`55 bp deleted → frameshift`
24.4	`GCGCGC--------------------------------------------------------GACCTCGGCTCACAGCGC`	`56 bp deleted → frameshift`
23.2	`GCGCGCCGGCGCCCCCTGGCGGC---------------------------------------------------------`	`57 bp deleted → no frameshift`
22.0	`GCGCGCCGGCG----------------------------------------------------------GCTCACAGCGC`	`58 bp deleted → frameshift`
20.8	`GCGCGCCGGCGC-----------------------------------------------------------TCACAGCGC`	`59 bp deleted → frameshift`
20.8	`GCGCGCC-----------------------------------------------------------TCGGCTCACAGCGC`	`59 bp deleted → frameshift`
20.0	`GCGCGCCGGCGCCCCCTGGC------------------------------------------------------------`	`60 bp deleted → no frameshift`
20.0	`GCGCGC--------------------------------------------------------------------------`	`74 bp deleted → frameshift`
19.2	`GCGCGCCGGCGCCCCCT-------------------------------------------------------CACAGCGC`	`55 bp deleted → frameshift`
17.6	`GCGC----------------------------------------------------------------------------`	`76 bp deleted → frameshift`
16.4	`GCGCG----------------------------------------------------------------GCTCACAGCGC`	`64 bp deleted → frameshift`
15.6	`GCGCGC-----------------------------------------------------------------TCACAGCGC`	`65 bp deleted → frameshift`
14.8	`GCGCGCCGGCGC------------------------------------------------------------------GC`	`66 bp deleted → no frameshift`
14.8	`GCG------------------------------------------------------------------GCTCACAGCGC`	`66 bp deleted → no frameshift`
14.0	`GCGC-------------------------------------------------------------------TCACAGCGC`	`67 bp deleted → frameshift`
12.8	`GC---------------------------------------------------------------------TCACAGCGC`	`69 bp deleted → no frameshift`
12.0	`GCGCGCCGGC----------------------------------------------------------------------`	`70 bp deleted → frameshift`
11.6	`GCGCGCCG-----------------------------------------------------------------------C`	`71 bp deleted → frameshift`
10.8	`GCGCGC------------------------------------------------------------------------GC`	`72 bp deleted → no frameshift`
8.0	`GC------------------------------------------------------------------------------`	`78 bp deleted → no frameshift`

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