CRISPOR

Micro-homology scoring of potential deletions

Guide: GATGGAGGGGAAGACGGCCC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	(Wild-type sequence)
397.6	`TGGTGCCTGGGGCGCCCCACGATGGAGGGG--------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`14 bp deleted → frameshift`
382.8	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC---------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`9 bp deleted → no frameshift`
377.6	`TGGTGCCTGGGGCGCCCCACGATGGAGGGG---------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`15 bp deleted → no frameshift`
359.2	`TGGTGCCTGGGGCGCCCCACGATGGAGGGG----------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`16 bp deleted → frameshift`
341.6	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCC-----------------GCGAAGCCGGCCTTGCACATGCC`	`17 bp deleted → frameshift`
313.2	`TGGTGCCTGGGGCGCCCCACGATGGAGGGG-------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`13 bp deleted → frameshift`
311.6	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG-----GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`5 bp deleted → frameshift`
296.4	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`6 bp deleted → no frameshift`
282.0	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG-------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`7 bp deleted → frameshift`
268.0	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG--------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`8 bp deleted → frameshift`
256.2	`TGGTGCCTGGGGCGCCCCACGATGGAGGG-----------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`17 bp deleted → frameshift`
223.0	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC------------------------------TTGCACATGCC`	`30 bp deleted → no frameshift`
219.6	`TGGTGCCTGGGGCGCCCCACGATGGAGGGG------------GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`12 bp deleted → no frameshift`
188.8	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG---------------TCGCCCGCGAAGCCGGCCTTGCACATGCC`	`15 bp deleted → no frameshift`
183.0	`TGGTGCCTGGGGC----------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`34 bp deleted → frameshift`
179.6	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCC----------------CGCGAAGCCGGCCTTGCACATGCC`	`16 bp deleted → frameshift`
170.8	`TGGTGCCTGGGGCGCCCCACGATGG-----------------GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`17 bp deleted → frameshift`
163.8	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC--------------------------GGCCTTGCACATGCC`	`26 bp deleted → frameshift`
162.8	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC------------------GCGAAGCCGGCCTTGCACATGCC`	`18 bp deleted → no frameshift`
162.8	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG------------------CCCGCGAAGCCGGCCTTGCACATGCC`	`18 bp deleted → no frameshift`
162.8	`TGGTGCCTGGGGCGCCCCACGATGGAGG------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`18 bp deleted → no frameshift`
162.8	`TGGTGCCTGGGGCGCCCCACGATGG------------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`18 bp deleted → no frameshift`
161.6	`TGGTGCCTGGGG--------------------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`32 bp deleted → frameshift`
154.8	`TGGTGCCTGGGGCGCCCCACGATGG-------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`19 bp deleted → frameshift`
153.6	`TGGTGCCTGGGG---------------------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`33 bp deleted → no frameshift`
147.2	`TGGTGCCTGGGGCGCCCCACGATGG--------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`20 bp deleted → frameshift`
140.0	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC---------------------GAAGCCGGCCTTGCACATGCC`	`21 bp deleted → no frameshift`
140.0	`TGGTGCCTGGGGCGCCCCACGATGG---------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`21 bp deleted → no frameshift`
135.0	`TGGTGCCTGGGGCGCCC----------------------------------------GCGAAGCCGGCCTTGCACATGCC`	`40 bp deleted → frameshift`
133.8	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAG------------------------------CCGGCCTTGCACATGCC`	`30 bp deleted → no frameshift`
133.2	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG----------------------CGAAGCCGGCCTTGCACATGCC`	`22 bp deleted → frameshift`
127.2	`TGGTGCCTGGGG-------------------------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`31 bp deleted → frameshift`
120.4	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG------------------------AAGCCGGCCTTGCACATGCC`	`24 bp deleted → no frameshift`
114.8	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCC-------------------------GGCCTTGCACATGCC`	`25 bp deleted → frameshift`
104.4	`TGGTGCCTGGG-----------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`35 bp deleted → frameshift`
94.0	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCC-----------------------------TTGCACATGCC`	`29 bp deleted → frameshift`
89.2	`TGGTGCCTGGGGCGCCCCACG------------------------------TCGCCCGCGAAGCCGGCCTTGCACATGCC`	`30 bp deleted → no frameshift`
89.2	`TGGTGCCTGGGG------------------------------GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`30 bp deleted → no frameshift`
85.2	`TGGTGCCTGGGGCGCCCC---------------------------------------GCGAAGCCGGCCTTGCACATGCC`	`39 bp deleted → no frameshift`
80.8	`TGGTGCCTGGGGCGC--------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`32 bp deleted → frameshift`
77.7	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGA---------------------------AGCCGGCCTTGCACATGCC`	`27 bp deleted → no frameshift`
77.4	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC-----------------------------------------`	`41 bp deleted → frameshift`
76.8	`TGGTGCCTGGGGCGCCCCACG---------------------------------CCCGCGAAGCCGGCCTTGCACATGCC`	`33 bp deleted → no frameshift`
71.0	`TGGTGCCTGGGGCGCCCCACGA---------------------------------------AGCCGGCCTTGCACATGCC`	`39 bp deleted → no frameshift`
70.5	`TGGTGCCTGGGGCGCCCCA-----------------------------TCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`29 bp deleted → frameshift`
69.6	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC-----------------------------------ACATGCC`	`35 bp deleted → frameshift`
66.6	`TGGTGCCTGGGGCGC--------------------------------------------GAAGCCGGCCTTGCACATGCC`	`44 bp deleted → frameshift`
66.0	`TGGTGCCTGG------------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`36 bp deleted → no frameshift`
62.8	`TGGTGCCTGGGGCGCCCCACGATGGAGGGG-------------------------------------CCTTGCACATGCC`	`37 bp deleted → frameshift`
62.8	`TGGTGCCTGGGGCGCCCCACG-------------------------------------CGAAGCCGGCCTTGCACATGCC`	`37 bp deleted → frameshift`
62.8	`TGGTGCCTGGGGCG-------------------------------------TCGCCCGCGAAGCCGGCCTTGCACATGCC`	`37 bp deleted → frameshift`
60.0	`TGGTGCCTGGGGCGCCCCACGATGGAGGG--------------------------------------CCTTGCACATGCC`	`38 bp deleted → frameshift`
60.0	`TGGTGCCTGGGGCGCCCC--------------------------------------CGCGAAGCCGGCCTTGCACATGCC`	`38 bp deleted → frameshift`
60.0	`TGGTGCCTGGGGCG----------------------------------------------AAGCCGGCCTTGCACATGCC`	`46 bp deleted → frameshift`
56.8	`TGGTGCCTGGGGCGCCCCACGATGGAGG---------------------------------------CCTTGCACATGCC`	`39 bp deleted → no frameshift`
56.8	`TGG---------------------------------------GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`39 bp deleted → no frameshift`
54.6	`TGGTGCCTGGGGCGCCCCACGAT--------------------------CGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`26 bp deleted → frameshift`
54.0	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCC----------------------------------------`	`40 bp deleted → frameshift`
54.0	`TGG----------------------------------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`40 bp deleted → frameshift`
52.2	`TGGTGCCTGGGGCGCCCCACGATGGA-----------------------------------AGCCGGCCTTGCACATGCC`	`35 bp deleted → frameshift`
51.6	`TGGTGCCTGGGGCGCC-----------------------------------------GCGAAGCCGGCCTTGCACATGCC`	`41 bp deleted → frameshift`
51.6	`TGGTGC-----------------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`41 bp deleted → frameshift`
51.6	`TGG-----------------------------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`41 bp deleted → frameshift`
51.6	`TGGTGCCTGGGGCGCC-------------------------------------------------GGCCTTGCACATGCC`	`49 bp deleted → frameshift`
51.6	`TGGTGCC-------------------------------------------------CGCGAAGCCGGCCTTGCACATGCC`	`49 bp deleted → frameshift`
49.5	`TGGTGCCTGGGGCGCCCCACGATGGAG------------------------------------CCGGCCTTGCACATGCC`	`36 bp deleted → no frameshift`
48.8	`TGGTGCCTGGGGCGCCCCACGATGG------------------------------------------CCTTGCACATGCC`	`42 bp deleted → no frameshift`
48.8	`TGGTGCCTGGGGC------------------------------------------CCGCGAAGCCGGCCTTGCACATGCC`	`42 bp deleted → no frameshift`
48.8	`TGG------------------------------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`42 bp deleted → no frameshift`
46.4	`TGG-------------------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCC`	`43 bp deleted → frameshift`
42.6	`TGGTGCCTGGGGCGCC-----------------------------------------------------TTGCACATGCC`	`53 bp deleted → frameshift`
42.0	`TGGTGCCTGGGGCGCCCCACG---------------------------------------------GCCTTGCACATGCC`	`45 bp deleted → no frameshift`
40.5	`TGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGAC----------------------------------------ATGCC`	`40 bp deleted → frameshift`
38.4	`TGGTGCCTGGGGC-------------------------------------------------------CTTGCACATGCC`	`55 bp deleted → frameshift`
38.0	`TGGTGCCTGGGGCGCCCC-----------------------------------------------GGCCTTGCACATGCC`	`47 bp deleted → frameshift`
36.4	`TGGTGCCTGGGGCGCCC------------------------------------------------GGCCTTGCACATGCC`	`48 bp deleted → no frameshift`
33.0	`TGGTGCC----------------------------------------------------------GGCCTTGCACATGCC`	`58 bp deleted → frameshift`
32.8	`TGGTGCC--------------------------------------------------GCGAAGCCGGCCTTGCACATGCC`	`50 bp deleted → frameshift`
32.0	`TGGTGCCTGGGGCGCCCCAC-------------------------------------------------------ATGCC`	`55 bp deleted → frameshift`
31.5	`TGGTGCCT--------------------------------------------------------------TGCACATGCC`	`62 bp deleted → frameshift`
31.2	`TGGTGCCTGGGGCGCCCC---------------------------------------------------TTGCACATGCC`	`51 bp deleted → no frameshift`
31.2	`TGGTGCCTGGGGC---------------------------------------------------CGGCCTTGCACATGCC`	`51 bp deleted → no frameshift`
29.6	`TGGTGCCTGGGGCGCCC----------------------------------------------------TTGCACATGCC`	`52 bp deleted → frameshift`
29.6	`TGGTGCCTGGGGCG----------------------------------------------------GCCTTGCACATGCC`	`52 bp deleted → frameshift`
28.4	`TGGTGC-----------------------------------------------------GAAGCCGGCCTTGCACATGCC`	`53 bp deleted → frameshift`
27.3	`TGGTGCCTGGGGCGCCCCACGATG------------------------------------------------CACATGCC`	`48 bp deleted → no frameshift`
27.3	`TGGT------------------------------------------------CGCCCGCGAAGCCGGCCTTGCACATGCC`	`48 bp deleted → no frameshift`
26.8	`TGGTGCCTGGGGCGCCCCACGATG------------------------------------------------------CC`	`54 bp deleted → no frameshift`
24.6	`TGGTGCCTGGGGCGCC----------------------------------------------------------------`	`64 bp deleted → frameshift`
24.4	`TGGTGCCTGGG--------------------------------------------------------CCTTGCACATGCC`	`56 bp deleted → frameshift`
23.2	`TGGTGCCTGG---------------------------------------------------------CCTTGCACATGCC`	`57 bp deleted → no frameshift`
22.0	`TGGTGCCTGGGGCGC----------------------------------------------------------ACATGCC`	`58 bp deleted → frameshift`
20.0	`TGGTGCCTGGGGC------------------------------------------------------------ACATGCC`	`60 bp deleted → no frameshift`
18.2	`TGGTGCC-------------------------------------------------------------------------`	`73 bp deleted → frameshift`
18.0	`TGGTGCCTGGGGCGCCCC--------------------------------------------------------------`	`62 bp deleted → frameshift`
17.5	`TGGTGC-------------------------------------------------------------------ACATGCC`	`67 bp deleted → frameshift`
17.4	`TGGTGCCTGGGGCGCCCCA---------------------------------------------------------TGCC`	`57 bp deleted → no frameshift`
17.2	`TGGTGCCTGGGGCGCCC---------------------------------------------------------------`	`63 bp deleted → no frameshift`
16.4	`TGG----------------------------------------------------------------CCTTGCACATGCC`	`64 bp deleted → frameshift`
14.8	`TGGTGCCTGGGGC------------------------------------------------------------------C`	`66 bp deleted → no frameshift`
12.9	`TGGTGCCTG---------------------------------------------------------------CACATGCC`	`63 bp deleted → no frameshift`
9.6	`TGGTGCCTG---------------------------------------------------------------------CC`	`69 bp deleted → no frameshift`
9.0	`TG----------------------------------------------------------------------CACATGCC`	`70 bp deleted → frameshift`
6.6	`TG----------------------------------------------------------------------------CC`	`76 bp deleted → frameshift`

Version 4.7 - Documentation - Contact us - Downloads/local installation - Paper - License