CRISPOR

Micro-homology scoring of potential deletions

Guide: CCCCTGGCGGCCTAAGGACT CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	(Wild-type sequence)
417.6	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGC-------------GCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`13 bp deleted → frameshift`
359.2	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCG----------------CGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`16 bp deleted → frameshift`
329.4	`ATCTGGGCGCGCGCCGG----------------------------------AAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`34 bp deleted → frameshift`
327.6	`ATCTGGGCGCGCGCCGGCGC--------------------------GCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`26 bp deleted → frameshift`
296.4	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGG------CGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`6 bp deleted → no frameshift`
256.2	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCC-----------------GGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`17 bp deleted → frameshift`
248.5	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGGA--------------AGTGGCCAGGGCGGGGGCGACCTCGGCT`	`14 bp deleted → frameshift`
247.0	`ATCTGGGCGCGCGCCGGCGCC----------------------------GGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`28 bp deleted → frameshift`
244.2	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCG------------------CCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`18 bp deleted → no frameshift`
231.0	`ATCTGGGCGCGC------------------------------------CGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`36 bp deleted → no frameshift`
210.0	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGG---------------------AAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`21 bp deleted → no frameshift`
207.2	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCC---------------------------AGGGCGGGGGCGACCTCGGCT`	`27 bp deleted → no frameshift`
188.8	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGC---------------GCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`15 bp deleted → no frameshift`
165.0	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGG------------------------------------GGGCGACCTCGGCT`	`36 bp deleted → no frameshift`
162.8	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAG------------------TGGCCAGGGCGGGGGCGACCTCGGCT`	`18 bp deleted → no frameshift`
161.6	`ATCTGGGCGCGCGC--------------------------------GCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`32 bp deleted → frameshift`
156.1	`ATCTGGGCGCGCGCCGGCGCCCCCTGGC------------------------------CAGGGCGGGGGCGACCTCGGCT`	`30 bp deleted → no frameshift`
147.2	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGG--------------------CCAGGGCGGGGGCGACCTCGGCT`	`20 bp deleted → frameshift`
147.2	`ATCTGGGCGCGCGCCGGCGCCCCCTGGC--------------------CGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`20 bp deleted → frameshift`
143.5	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGG-------------------------GCGGGGGCGACCTCGGCT`	`25 bp deleted → frameshift`
126.8	`ATCTGGGCGCGCGCCGGCG-----------------------GCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`23 bp deleted → frameshift`
120.4	`ATCTGGGCGCGCGCCGGCGCCCCCTGG------------------------AAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`24 bp deleted → no frameshift`
120.4	`ATCTGGGCGCGCGCCGGCGC------------------------GCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`24 bp deleted → no frameshift`
120.0	`ATCTGGGCGC--------------------------------------CGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`38 bp deleted → frameshift`
115.2	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGC---------------------------------GGGGGCGACCTCGGCT`	`33 bp deleted → no frameshift`
114.8	`ATCTGGGCGCGCGCCGGCGCCCCC-------------------------GGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`25 bp deleted → frameshift`
109.2	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGG--------------------------CGGGGGCGACCTCGGCT`	`26 bp deleted → frameshift`
109.2	`ATCTGGGCGCGCGCCGGCGCCCC--------------------------GGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`26 bp deleted → frameshift`
103.6	`ATCTGGGCGCGCGCCGGCGCCC---------------------------GGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`27 bp deleted → no frameshift`
97.6	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCG------------------------------------------ACCTCGGCT`	`42 bp deleted → no frameshift`
94.0	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGG-----------------------------GGGCGACCTCGGCT`	`29 bp deleted → frameshift`
94.0	`ATCTGGGCGCGCGCCG-----------------------------CGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`29 bp deleted → frameshift`
94.0	`ATCTGGGCGCGCG-----------------------------GCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`29 bp deleted → frameshift`
91.5	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGGAC----------------------------------CTCGGCT`	`34 bp deleted → frameshift`
90.0	`ATCTGGGCGCGCGCCGGCGCC--------------------------------------AGGGCGGGGGCGACCTCGGCT`	`38 bp deleted → frameshift`
89.2	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGG------------------------------GGCGACCTCGGCT`	`30 bp deleted → no frameshift`
89.2	`ATCTGGGCGCGCGCCGGC------------------------------CGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`30 bp deleted → no frameshift`
89.2	`ATCTGGGCGCGCGC------------------------------GCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`30 bp deleted → no frameshift`
85.2	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGC---------------------------------------GACCTCGGCT`	`39 bp deleted → no frameshift`
84.8	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGG-------------------------------GCGACCTCGGCT`	`31 bp deleted → frameshift`
84.8	`ATCTGGGCGCGCGCCGGCG-------------------------------GAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`31 bp deleted → frameshift`
84.8	`ATCTGGGCGCGCGCCG-------------------------------CCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`31 bp deleted → frameshift`
84.8	`ATCTGGGCGCG-------------------------------GCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`31 bp deleted → frameshift`
81.0	`ATCTGGGCGCGCGCCGGC----------------------------------------CAGGGCGGGGGCGACCTCGGCT`	`40 bp deleted → frameshift`
80.8	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGG--------------------------------CGACCTCGGCT`	`32 bp deleted → frameshift`
80.8	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGG--------------------------------GCGGGGGCGACCTCGGCT`	`32 bp deleted → frameshift`
80.0	`ATCTGGGCGCGCGCCGGCG----------------------------------------------GGGGCGACCTCGGCT`	`46 bp deleted → frameshift`
76.8	`ATCTGGGCG---------------------------------GCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`33 bp deleted → no frameshift`
72.8	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGC------------------------------------------------T`	`48 bp deleted → no frameshift`
69.6	`ATCTGGGCGCGCGCCGGCGCCCCCTGG-----------------------------------GCGGGGGCGACCTCGGCT`	`35 bp deleted → frameshift`
69.6	`ATCTGGGCGCGCGCCGGCGCCCCC-----------------------------------AGGGCGGGGGCGACCTCGGCT`	`35 bp deleted → frameshift`
66.6	`ATCTGGGCGCGCGCC--------------------------------------------AGGGCGGGGGCGACCTCGGCT`	`44 bp deleted → frameshift`
66.0	`ATCTGGGCGCGCGCCGGCGCCCC------------------------------------AGGGCGGGGGCGACCTCGGCT`	`36 bp deleted → no frameshift`
62.8	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGG-------------------------------------GGCGACCTCGGCT`	`37 bp deleted → frameshift`
62.8	`ATCTGGGCGCGCGCCGGCGCCC-------------------------------------AGGGCGGGGGCGACCTCGGCT`	`37 bp deleted → frameshift`
62.8	`ATCTGGGCGCGCG-------------------------------------GAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`37 bp deleted → frameshift`
62.8	`ATCTGG-------------------------------------CGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`37 bp deleted → frameshift`
61.0	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCT------------------------------------------CGGCT`	`42 bp deleted → no frameshift`
61.0	`ATCTGGGCG--------------------------------------------------------GGGGCGACCTCGGCT`	`56 bp deleted → frameshift`
60.0	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGG--------------------------------------GCGACCTCGGCT`	`38 bp deleted → frameshift`
59.2	`ATCTGGGCGCGCGCCGGCG----------------------------------------------------ACCTCGGCT`	`52 bp deleted → frameshift`
56.8	`ATCTGGGCGCGCGCCGGCGCCCCCTGG---------------------------------------GGGCGACCTCGGCT`	`39 bp deleted → no frameshift`
56.8	`ATCTGGGCGCG---------------------------------------GAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`39 bp deleted → no frameshift`
54.0	`ATCTGGGCGCGCGCCGGCGCCCCCTGG----------------------------------------GGCGACCTCGGCT`	`40 bp deleted → frameshift`
54.0	`ATCTGGGC----------------------------------------CGGAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`40 bp deleted → frameshift`
52.2	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGGACT-----------------------------------CGGCT`	`35 bp deleted → frameshift`
51.6	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGG-----------------------------------------CT`	`41 bp deleted → frameshift`
51.6	`ATCTGGGCGCGCGCCGGCGCCCCCTGG-----------------------------------------GCGACCTCGGCT`	`41 bp deleted → frameshift`
51.6	`ATCTGGGCG-----------------------------------------GAAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`41 bp deleted → frameshift`
51.6	`ATCTGGGCGCGCGCCGG-------------------------------------------------GGGCGACCTCGGCT`	`49 bp deleted → frameshift`
49.2	`ATCTGGGC--------------------------------------------------CAGGGCGGGGGCGACCTCGGCT`	`50 bp deleted → frameshift`
46.8	`ATCTGGGCGCGCGCCGGCGCCCCCTGGC---------------------------------------------------T`	`51 bp deleted → no frameshift`
45.0	`ATCTGGGCG--------------------------------------------------------------ACCTCGGCT`	`62 bp deleted → frameshift`
44.4	`ATCTGGGCGCGCGCCGGCGC--------------------------------------------GGGGGCGACCTCGGCT`	`44 bp deleted → frameshift`
44.4	`ATCTGGGCGCGCG----------------------------------------------------GGGGCGACCTCGGCT`	`52 bp deleted → frameshift`
44.4	`ATCTGGG--------------------------------------------AAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`44 bp deleted → frameshift`
42.0	`ATCTGGGCGCGCGCCGG---------------------------------------------GCGGGGGCGACCTCGGCT`	`45 bp deleted → no frameshift`
42.0	`ATCTGG---------------------------------------------AAGTGGCCAGGGCGGGGGCGACCTCGGCT`	`45 bp deleted → no frameshift`
41.0	`ATCTGGGCGCGCGCCGGCGCCCCCT--------------------------------------------------CGGCT`	`50 bp deleted → frameshift`
40.5	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGGACT----------------------------------------`	`40 bp deleted → frameshift`
40.2	`ATCTGGGCGCG------------------------------------------------------GGGGCGACCTCGGCT`	`54 bp deleted → no frameshift`
40.0	`ATCTGGGCGCGC----------------------------------------------CAGGGCGGGGGCGACCTCGGCT`	`46 bp deleted → frameshift`
39.0	`ATCTGG---------------------------------------------------CCAGGGCGGGGGCGACCTCGGCT`	`51 bp deleted → no frameshift`
37.6	`ATCTGGGCGCGCGCCGGC-------------------------------------------------------------T`	`61 bp deleted → frameshift`
36.4	`ATCTGGGCGC------------------------------------------------CAGGGCGGGGGCGACCTCGGCT`	`48 bp deleted → no frameshift`
33.0	`ATCTGGGCGCGCG----------------------------------------------------------ACCTCGGCT`	`58 bp deleted → frameshift`
32.8	`ATCTGGGCGCGCGCCGGCGC--------------------------------------------------GACCTCGGCT`	`50 bp deleted → frameshift`
32.8	`ATCTGGGCGCGCGCCGG--------------------------------------------------GGCGACCTCGGCT`	`50 bp deleted → frameshift`
32.8	`ATCTGGGCGCGCGC--------------------------------------------------GGGGGCGACCTCGGCT`	`50 bp deleted → frameshift`
31.2	`ATCTGGGCGCGCGCCGGCGCCCC---------------------------------------------------TCGGCT`	`51 bp deleted → no frameshift`
31.2	`ATCTGGGCGCGCGCCGG---------------------------------------------------GCGACCTCGGCT`	`51 bp deleted → no frameshift`
30.0	`ATCTGGGCGCG------------------------------------------------------------ACCTCGGCT`	`60 bp deleted → no frameshift`
30.0	`ATCTGGG------------------------------------------------------------GGCGACCTCGGCT`	`60 bp deleted → no frameshift`
29.6	`ATCTGGGCGCGCGCCGGCGCCC----------------------------------------------------TCGGCT`	`52 bp deleted → frameshift`
28.5	`ATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCT-----------------------------------------------`	`47 bp deleted → frameshift`
28.4	`ATCTGGGCGCGCGCCGGCGCC-----------------------------------------------------TCGGCT`	`53 bp deleted → frameshift`
28.2	`ATCTGGG-------------------------------------------------------------GCGACCTCGGCT`	`61 bp deleted → frameshift`
25.6	`ATCTGGGCGCGCGCCG-------------------------------------------------------ACCTCGGCT`	`55 bp deleted → frameshift`
25.6	`ATCTGGG-------------------------------------------------------GCGGGGGCGACCTCGGCT`	`55 bp deleted → frameshift`
24.4	`ATCTGGGCGCGCGC--------------------------------------------------------GACCTCGGCT`	`56 bp deleted → frameshift`
23.2	`ATCTGG---------------------------------------------------------CGGGGGCGACCTCGGCT`	`57 bp deleted → no frameshift`
22.0	`ATCTGGGCGCGCGCCGGCG----------------------------------------------------------GCT`	`58 bp deleted → frameshift`
20.8	`ATCTGGGCGCGCGCCGGCGC-----------------------------------------------------------T`	`59 bp deleted → frameshift`
20.8	`ATCTGGGCGCGCGCC-----------------------------------------------------------TCGGCT`	`59 bp deleted → frameshift`
20.8	`ATCTGGG-----------------------------------------------------------GGGCGACCTCGGCT`	`59 bp deleted → frameshift`
19.2	`ATCTGGGCGCGCGCCGGCGCCCCCT-------------------------------------------------------`	`55 bp deleted → frameshift`
17.4	`ATCTGGGC-----------------------------------------------------------------------T`	`71 bp deleted → frameshift`
17.2	`ATCTGG---------------------------------------------------------------CGACCTCGGCT`	`63 bp deleted → no frameshift`
16.4	`ATCTGGGCGCGCG----------------------------------------------------------------GCT`	`64 bp deleted → frameshift`
15.6	`ATCTGGGCGCGCGC-----------------------------------------------------------------T`	`65 bp deleted → frameshift`
14.8	`ATCTGGGCGCG------------------------------------------------------------------GCT`	`66 bp deleted → no frameshift`
14.0	`ATCTGGGCGCGC-------------------------------------------------------------------T`	`67 bp deleted → frameshift`
13.2	`ATCTGGGCG--------------------------------------------------------------------GCT`	`68 bp deleted → frameshift`
12.8	`ATCTGGGCGC---------------------------------------------------------------------T`	`69 bp deleted → no frameshift`
10.8	`ATCTGG------------------------------------------------------------------------CT`	`72 bp deleted → no frameshift`
8.7	`ATCT-----------------------------------------------------------------------CGGCT`	`71 bp deleted → frameshift`
7.8	`ATC-------------------------------------------------------------------------GGCT`	`73 bp deleted → frameshift`
6.6	`ATCT----------------------------------------------------------------------------`	`76 bp deleted → frameshift`

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