CRISPOR

Micro-homology scoring of potential deletions

Guide: CGATGGAGGGGAAGACGGCC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	(Wild-type sequence)
467.4	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG-----GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`5 bp deleted → frameshift`
397.6	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGG--------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`14 bp deleted → frameshift`
382.8	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC---------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`9 bp deleted → no frameshift`
377.6	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGG---------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`15 bp deleted → no frameshift`
359.2	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGG----------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`16 bp deleted → frameshift`
313.2	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGG-------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`13 bp deleted → frameshift`
296.4	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`6 bp deleted → no frameshift`
282.0	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG-------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`7 bp deleted → frameshift`
268.0	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG--------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`8 bp deleted → frameshift`
256.2	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC-----------------CGCGAAGCCGGCCTTGCACATGC`	`17 bp deleted → frameshift`
256.2	`CTGGTGCCTGGGGCGCCCCACGATGGAGGG-----------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`17 bp deleted → frameshift`
223.0	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC------------------------------TTGCACATGC`	`30 bp deleted → no frameshift`
219.6	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGG------------GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`12 bp deleted → no frameshift`
188.8	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG---------------TCGCCCGCGAAGCCGGCCTTGCACATGC`	`15 bp deleted → no frameshift`
183.0	`CTGGTGCCTGGGGC----------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`34 bp deleted → frameshift`
170.8	`CTGGTGCCTGGGGCGCCCCACGATGG-----------------GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`17 bp deleted → frameshift`
163.8	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC--------------------------GGCCTTGCACATGC`	`26 bp deleted → frameshift`
162.8	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC------------------GCGAAGCCGGCCTTGCACATGC`	`18 bp deleted → no frameshift`
162.8	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG------------------CCCGCGAAGCCGGCCTTGCACATGC`	`18 bp deleted → no frameshift`
162.8	`CTGGTGCCTGGGGCGCCCCACGATGGAGG------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`18 bp deleted → no frameshift`
162.8	`CTGGTGCCTGGGGCGCCCCACGATGG------------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`18 bp deleted → no frameshift`
161.6	`CTGGTGCCTGGGG--------------------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`32 bp deleted → frameshift`
154.8	`CTGGTGCCTGGGGCGCCCCACGATGG-------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`19 bp deleted → frameshift`
153.6	`CTGGTGCCTGGGG---------------------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`33 bp deleted → no frameshift`
147.2	`CTGGTGCCTGGGGCGCCCCACGATGG--------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`20 bp deleted → frameshift`
147.2	`CTGGTGCCTGGGGCGCCCCACG--------------------GGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`20 bp deleted → frameshift`
140.0	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC---------------------GAAGCCGGCCTTGCACATGC`	`21 bp deleted → no frameshift`
140.0	`CTGGTGCCTGGGGCGCCCCACGATGG---------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`21 bp deleted → no frameshift`
135.0	`CTGGTGCCTGGGGCGCCC----------------------------------------GCGAAGCCGGCCTTGCACATGC`	`40 bp deleted → frameshift`
133.8	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAG------------------------------CCGGCCTTGCACATGC`	`30 bp deleted → no frameshift`
133.2	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG----------------------CGAAGCCGGCCTTGCACATGC`	`22 bp deleted → frameshift`
127.2	`CTGGTGCCTGGGG-------------------------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`31 bp deleted → frameshift`
120.4	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACG------------------------AAGCCGGCCTTGCACATGC`	`24 bp deleted → no frameshift`
104.4	`CTGGTGCCTGGG-----------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`35 bp deleted → frameshift`
103.6	`CTGGTGCCTGGGGCG---------------------------GGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`27 bp deleted → no frameshift`
89.2	`CTGGTGCCTGGGGCGCCCCACG------------------------------TCGCCCGCGAAGCCGGCCTTGCACATGC`	`30 bp deleted → no frameshift`
89.2	`CTGGTGCCTGGGG------------------------------GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`30 bp deleted → no frameshift`
85.2	`CTGGTGCCTGGGGCGCCCC---------------------------------------GCGAAGCCGGCCTTGCACATGC`	`39 bp deleted → no frameshift`
80.8	`CTGGTGCCTGGGGCGC--------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`32 bp deleted → frameshift`
77.7	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGA---------------------------AGCCGGCCTTGCACATGC`	`27 bp deleted → no frameshift`
76.8	`CTGGTGCCTGGGGCGCCCCACG---------------------------------CCCGCGAAGCCGGCCTTGCACATGC`	`33 bp deleted → no frameshift`
71.0	`CTGGTGCCTGGGGCGCCCCACGA---------------------------------------AGCCGGCCTTGCACATGC`	`39 bp deleted → no frameshift`
70.5	`CTGGTGCCTGGGGCGCCCCA-----------------------------TCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`29 bp deleted → frameshift`
69.6	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC-----------------------------------ACATGC`	`35 bp deleted → frameshift`
66.6	`CTGGTGCCTGGGGCGC--------------------------------------------GAAGCCGGCCTTGCACATGC`	`44 bp deleted → frameshift`
66.0	`CTGGTGCCTGG------------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`36 bp deleted → no frameshift`
62.8	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGG-------------------------------------CCTTGCACATGC`	`37 bp deleted → frameshift`
62.8	`CTGGTGCCTGGGGCGCCCCACG-------------------------------------CGAAGCCGGCCTTGCACATGC`	`37 bp deleted → frameshift`
62.8	`CTGGTGCCTGGGGCG-------------------------------------TCGCCCGCGAAGCCGGCCTTGCACATGC`	`37 bp deleted → frameshift`
60.0	`CTGGTGCCTGGGGCGCCCCACGATGGAGGG--------------------------------------CCTTGCACATGC`	`38 bp deleted → frameshift`
60.0	`CTGGTGCCTGGGGCGCCCC--------------------------------------CGCGAAGCCGGCCTTGCACATGC`	`38 bp deleted → frameshift`
60.0	`CTGGTGCCTGGGGCG----------------------------------------------AAGCCGGCCTTGCACATGC`	`46 bp deleted → frameshift`
56.8	`CTGGTGCCTGGGGCGCCCCACGATGGAGG---------------------------------------CCTTGCACATGC`	`39 bp deleted → no frameshift`
56.8	`CTGG---------------------------------------GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`39 bp deleted → no frameshift`
54.6	`CTGGTGCCTGGGGCGCCCCACGAT--------------------------CGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`26 bp deleted → frameshift`
54.0	`CTGG----------------------------------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`40 bp deleted → frameshift`
52.2	`CTGGTGCCTGGGGCGCCCCACGATGGA-----------------------------------AGCCGGCCTTGCACATGC`	`35 bp deleted → frameshift`
51.6	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC-----------------------------------------`	`41 bp deleted → frameshift`
51.6	`CTGGTGCCTGGGGCGCC-----------------------------------------GCGAAGCCGGCCTTGCACATGC`	`41 bp deleted → frameshift`
51.6	`CTGGTGC-----------------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`41 bp deleted → frameshift`
51.6	`CTGG-----------------------------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`41 bp deleted → frameshift`
51.6	`CTGGTGCCTGGGGCGCC-------------------------------------------------GGCCTTGCACATGC`	`49 bp deleted → frameshift`
51.6	`CTGGTGCC-------------------------------------------------CGCGAAGCCGGCCTTGCACATGC`	`49 bp deleted → frameshift`
49.5	`CTGGTGCCTGGGGCGCCCCACGATGGAG------------------------------------CCGGCCTTGCACATGC`	`36 bp deleted → no frameshift`
48.8	`CTGGTGCCTGGGGCGCCCCACGATGG------------------------------------------CCTTGCACATGC`	`42 bp deleted → no frameshift`
48.8	`CTGGTGCCTGGGGC------------------------------------------CCGCGAAGCCGGCCTTGCACATGC`	`42 bp deleted → no frameshift`
48.8	`CTGG------------------------------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`42 bp deleted → no frameshift`
46.4	`CTGG-------------------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGC`	`43 bp deleted → frameshift`
42.6	`CTGGTGCCTGGGGCGCC-----------------------------------------------------TTGCACATGC`	`53 bp deleted → frameshift`
42.0	`CTGGTGCCTGGGGCGCCCCACG---------------------------------------------GCCTTGCACATGC`	`45 bp deleted → no frameshift`
40.5	`CTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGAC----------------------------------------ATGC`	`40 bp deleted → frameshift`
38.4	`CTGGTGCCTGGGGC-------------------------------------------------------CTTGCACATGC`	`55 bp deleted → frameshift`
38.0	`CTGGTGCCTGGGGCGCCCC-----------------------------------------------GGCCTTGCACATGC`	`47 bp deleted → frameshift`
36.4	`CTGGTGCCTGGGGCGCCC------------------------------------------------GGCCTTGCACATGC`	`48 bp deleted → no frameshift`
33.0	`CTGGTGCC----------------------------------------------------------GGCCTTGCACATGC`	`58 bp deleted → frameshift`
32.8	`CTGGTGCC--------------------------------------------------GCGAAGCCGGCCTTGCACATGC`	`50 bp deleted → frameshift`
32.0	`CTGGTGCCTGGGGCGCCCCAC-------------------------------------------------------ATGC`	`55 bp deleted → frameshift`
31.5	`CTGGTGCCT--------------------------------------------------------------TGCACATGC`	`62 bp deleted → frameshift`
31.2	`CTGGTGCCTGGGGCGCCCC---------------------------------------------------TTGCACATGC`	`51 bp deleted → no frameshift`
31.2	`CTGGTGCCTGGGGC---------------------------------------------------CGGCCTTGCACATGC`	`51 bp deleted → no frameshift`
29.6	`CTGGTGCCTGGGGCGCCC----------------------------------------------------TTGCACATGC`	`52 bp deleted → frameshift`
29.6	`CTGGTGCCTGGGGCG----------------------------------------------------GCCTTGCACATGC`	`52 bp deleted → frameshift`
28.4	`CTGGTGC-----------------------------------------------------GAAGCCGGCCTTGCACATGC`	`53 bp deleted → frameshift`
27.3	`CTGGTGCCTGGGGCGCCCCACGATG------------------------------------------------CACATGC`	`48 bp deleted → no frameshift`
27.3	`CTGGT------------------------------------------------CGCCCGCGAAGCCGGCCTTGCACATGC`	`48 bp deleted → no frameshift`
26.8	`CTGGTGCCTGGGGCGCCCCACGATG------------------------------------------------------C`	`54 bp deleted → no frameshift`
24.4	`CTGGTGCCTGGG--------------------------------------------------------CCTTGCACATGC`	`56 bp deleted → frameshift`
23.2	`CTGGTGCCTGG---------------------------------------------------------CCTTGCACATGC`	`57 bp deleted → no frameshift`
22.0	`CTGGTGCCTGGGGCGC----------------------------------------------------------ACATGC`	`58 bp deleted → frameshift`
20.0	`CTGGTGCCTGGGGC------------------------------------------------------------ACATGC`	`60 bp deleted → no frameshift`
17.5	`CTGGTGC-------------------------------------------------------------------ACATGC`	`67 bp deleted → frameshift`
17.4	`CTGGTGCCTGGGGCGCCCCA---------------------------------------------------------TGC`	`57 bp deleted → no frameshift`
16.4	`CTGGTGCCTGGGGCGC----------------------------------------------------------------`	`64 bp deleted → frameshift`
16.4	`CTGG----------------------------------------------------------------CCTTGCACATGC`	`64 bp deleted → frameshift`
14.8	`CTGGTGCCTGGGGC------------------------------------------------------------------`	`66 bp deleted → no frameshift`
13.0	`CTGGTGC-------------------------------------------------------------------------`	`73 bp deleted → frameshift`
12.9	`CTGGTGCCTG---------------------------------------------------------------CACATGC`	`63 bp deleted → no frameshift`
9.6	`CTGGTGCCTG---------------------------------------------------------------------C`	`69 bp deleted → no frameshift`
9.6	`CT---------------------------------------------------------------------TGCACATGC`	`69 bp deleted → no frameshift`
9.0	`CTG----------------------------------------------------------------------CACATGC`	`70 bp deleted → frameshift`
6.6	`CTG----------------------------------------------------------------------------C`	`76 bp deleted → frameshift`

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