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Contents:

PCR primers for off-targets of CCCCACGATGGAGGGGAAGA CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529549_F TCGTCGGCAGCGTCCTCCCCTACCTGGTGCCT 59.9 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529549_R GTCTCGTGGGCTCGGTGGATGATGATATCGCCGCG 60.4 1.00
mm2__chr15 43.99 Mbp_chr15_43990074_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 1.00
mm2__chr15 43.99 Mbp_chr15_43990074_R GTCTCGTGGGCTCGGTGTCGTCGACAATGGCTCTG 60.3 1.00
mm1__chr6 88.28 Mbp_chr6_88276899_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 1.00
mm1__chr6 88.28 Mbp_chr6_88276899_R GTCTCGTGGGCTCGGTGGATGATGCTATCACCGCG 60.3 1.00
mm0__chr19 9.52 Mbp_chr19_9518234_F TCGTCGGCAGCGTCAGTTAACAATGGCTCCGGCA 59.9 1.00
mm0__chr19 9.52 Mbp_chr19_9518234_R GTCTCGTGGGCTCGGGGAGTCCTTCTGACCGATGC 60.1 1.00
mm0__chr11 1.80 Mbp_chr11_1803987_F TCGTCGGCAGCGTCCGCCACCCACATAGAAGTCC 60.4 1.00
mm0__chr11 1.80 Mbp_chr11_1803987_R GTCTCGTGGGCTCGGAACACCTCTGGCATGTGCAA 60.4 1.00
mm1__chr22 41.08 Mbp_chr22_41075131_F TCGTCGGCAGCGTCGGAGTCCTTCTGACCCATGC 60.1 0.92
mm1__chr22 41.08 Mbp_chr22_41075131_R GTCTCGTGGGCTCGGTGTTGACAACAGCTCTGGCA 60.1 0.92
mm2__chr9 6.83 Mbp_chr9_6834539_F Primer3: not found at this Tm N.d. 0.86
mm2__chr9 6.83 Mbp_chr9_6834539_R Primer3: not found at this Tm N.d. 0.86
mm4__chr5 98.34 Mbp_chr5_98338827_F TCGTCGGCAGCGTCGCTCCTCATCAACAAGGGCT 60.0 0.80
mm4__chr5 98.34 Mbp_chr5_98338827_R GTCTCGTGGGCTCGGTTTTGACCCATGCCCACCAT 60.1 0.80
mm3__chr20 57.92 Mbp_chr20_57918620_F Primer3: not found at this Tm N.d. 0.74
mm3__chr20 57.92 Mbp_chr20_57918620_R Primer3: not found at this Tm N.d. 0.74
mm2__chr10 69.02 Mbp_chr10_69022838_F Primer3: not found at this Tm N.d. 0.71
mm2__chr10 69.02 Mbp_chr10_69022838_R Primer3: not found at this Tm N.d. 0.71
mm2__chr18 62.44 Mbp_chr18_62442093_F TCGTCGGCAGCGTCCCTCGTCATCGACAATGGCT 60.1 0.69
mm2__chr18 62.44 Mbp_chr18_62442093_R GTCTCGTGGGCTCGGGAGTCCTTCTGACCCATGCC 60.1 0.69
mm3__chr11 50.00 Mbp_chr11_49996392_F Primer3: not found at this Tm N.d. 0.68
mm3__chr11 50.00 Mbp_chr11_49996392_R Primer3: not found at this Tm N.d. 0.68
mm2__chr1 223.86 Mbp_chr1_223864743_F TCGTCGGCAGCGTCTGACCCATGCTCACCATCAC 60.0 0.63
mm2__chr1 223.86 Mbp_chr1_223864743_R GTCTCGTGGGCTCGGCGCTCGTCATTGACAACAGC 60.1 0.63
mm4__chr17 20.98 Mbp_chr17_20976512_F Primer3: not found at this Tm N.d. 0.60
mm4__chr17 20.98 Mbp_chr17_20976512_R Primer3: not found at this Tm N.d. 0.60
mm4__chr17 16.96 Mbp_chr17_16963354_F TCGTCGGCAGCGTCTAAGGAGTGCTGTGCTGGAC 59.6 0.60
mm4__chr17 16.96 Mbp_chr17_16963354_R GTCTCGTGGGCTCGGTTCACTCCTGCACAGACACC 59.8 0.60
mm4__chr22 29.49 Mbp_chr22_29490252_F TCGTCGGCAGCGTCAGCAAGAAAGAGGAGGCACC 59.9 0.58
mm4__chr22 29.49 Mbp_chr22_29490252_R GTCTCGTGGGCTCGGTCAGCCTCTTCCTTCTTGGC 59.6 0.58
mm4__chr18 23.24 Mbp_chr18_23239724_F Primer3: not found at this Tm N.d. 0.58
mm4__chr18 23.24 Mbp_chr18_23239724_R Primer3: not found at this Tm N.d. 0.58
mm4__chr14 50.12 Mbp_chr14_50117697_F Primer3: not found at this Tm N.d. 0.55
mm4__chr14 50.12 Mbp_chr14_50117697_R Primer3: not found at this Tm N.d. 0.55
mm3__chr6 41.54 Mbp_chr6_41537182_F Primer3: not found at this Tm N.d. 0.54
mm3__chr6 41.54 Mbp_chr6_41537182_R Primer3: not found at this Tm N.d. 0.54
mm4__chr10 7.64 Mbp_chr10_7638220_F Primer3: not found at this Tm N.d. 0.53
mm4__chr10 7.64 Mbp_chr10_7638220_R Primer3: not found at this Tm N.d. 0.53
mm2__chr5 131.66 Mbp_chr5_131660008_F TCGTCGGCAGCGTCATGCCCATCATCACACCCTG 60.1 0.53
mm2__chr5 131.66 Mbp_chr5_131660008_R GTCTCGTGGGCTCGGTGTGGAAGGTCTGCTTCACG 60.2 0.53
mm4__chr10 116.21 Mbp_chr10_116214402_F Primer3: not found at this Tm N.d. 0.49
mm4__chr10 116.21 Mbp_chr10_116214402_R Primer3: not found at this Tm N.d. 0.49
mm4__chr18 38.54 Mbp_chr18_38541461_F TCGTCGGCAGCGTCGGATAAGACCCATGTTCCTCCT 58.9 0.46
mm4__chr18 38.54 Mbp_chr18_38541461_R GTCTCGTGGGCTCGGAGATGCGTGGGTCTTGTGAG 60.0 0.46
mm4__chr5 155.58 Mbp_chr5_155575799_F Primer3: not found at this Tm N.d. 0.46
mm4__chr5 155.58 Mbp_chr5_155575799_R Primer3: not found at this Tm N.d. 0.46
mm4__chr7 157.85 Mbp_chr7_157849859_F TCGTCGGCAGCGTCGGGGAGGCCAAACGTGTTAT 60.3 0.46
mm4__chr7 157.85 Mbp_chr7_157849859_R GTCTCGTGGGCTCGGATCTCATGTCGTCCCCAGGA 60.0 0.46
mm4__chr10 65.84 Mbp_chr10_65840430_F Primer3: not found at this Tm N.d. 0.45
mm4__chr10 65.84 Mbp_chr10_65840430_R Primer3: not found at this Tm N.d. 0.45
mm4__chr20 63.70 Mbp_chr20_63703909_F Primer3: not found at this Tm N.d. 0.44
mm4__chr20 63.70 Mbp_chr20_63703909_R Primer3: not found at this Tm N.d. 0.44
mm4__chr5 1.07 Mbp_chr5_1068507_F TCGTCGGCAGCGTCGCCCAGATTTCTCTAATTTCACACT 59.3 0.43
mm4__chr5 1.07 Mbp_chr5_1068507_R GTCTCGTGGGCTCGGCCTTCCCTTGGCTGCCTTT 60.2 0.43
mm3__chr12 26.33 Mbp_chr12_26334147_F TCGTCGGCAGCGTCCCAAGATCACAAAGATACACCTG 57.1 0.42
mm3__chr12 26.33 Mbp_chr12_26334147_R GTCTCGTGGGCTCGGCCCTGCTCTAATGACTGTGCT 59.7 0.42
mm4__chr5 122.12 Mbp_chr5_122119183_F Primer3: not found at this Tm N.d. 0.42
mm4__chr5 122.12 Mbp_chr5_122119183_R Primer3: not found at this Tm N.d. 0.42
mm4__chrX 56.62 Mbp_chrX_56618391_F TCGTCGGCAGCGTCGCAGGGAAGGAAGCCAAGTA 59.6 0.42
mm4__chrX 56.62 Mbp_chrX_56618391_R GTCTCGTGGGCTCGGGCTTGTGAAGTGCCTTCCTC 59.1 0.42
mm4__chr3 113.14 Mbp_chr3_113141384_F Primer3: not found at this Tm N.d. 0.42
mm4__chr3 113.14 Mbp_chr3_113141384_R Primer3: not found at this Tm N.d. 0.42
mm4__chr3 69.10 Mbp_chr3_69097735_F TCGTCGGCAGCGTCGCAGAGAGGCCAAGTAGACC 59.8 0.41
mm4__chr3 69.10 Mbp_chr3_69097735_R GTCTCGTGGGCTCGGCCCAACCTTCCCCAATACCC 60.0 0.41
mm4__chr2 174.16 Mbp_chr2_174157340_F TCGTCGGCAGCGTCAGCCTAACAGGGGAGACAGA 59.5 0.41
mm4__chr2 174.16 Mbp_chr2_174157340_R GTCTCGTGGGCTCGGTGAAACCTTGAAGGCTGTCTT 57.7 0.41
mm4__chr1 18.55 Mbp_chr1_18546964_F Primer3: not found at this Tm N.d. 0.40
mm4__chr1 18.55 Mbp_chr1_18546964_R Primer3: not found at this Tm N.d. 0.40
mm4__chr8 84.95 Mbp_chr8_84948673_F TCGTCGGCAGCGTCCTCTGGTATGTGCAAGGCCA 60.0 0.39
mm4__chr8 84.95 Mbp_chr8_84948673_R GTCTCGTGGGCTCGGCAGTCCTTCTGCCCTATGCC 60.1 0.39
mm4__chr1 181.48 Mbp_chr1_181481588_F TCGTCGGCAGCGTCGTTTCCTTCCTCAGCCCCTC 60.0 0.38
mm4__chr1 181.48 Mbp_chr1_181481588_R GTCTCGTGGGCTCGGCTGTGTTTTCCTGCCTTGGC 59.9 0.38
mm4__chr12 21.72 Mbp_chr12_21717044_F Primer3: not found at this Tm N.d. 0.37
mm4__chr12 21.72 Mbp_chr12_21717044_R Primer3: not found at this Tm N.d. 0.37
mm3__chr6 101.43 Mbp_chr6_101431429_F TCGTCGGCAGCGTCTGATGCTGTCCTGTGTGCTT 59.8 0.37
mm3__chr6 101.43 Mbp_chr6_101431429_R GTCTCGTGGGCTCGGCGGCTTTGCTGGGGATGA 60.0 0.37
mm4__chr9 86.07 Mbp_chr9_86074493_F Primer3: not found at this Tm N.d. 0.36
mm4__chr9 86.07 Mbp_chr9_86074493_R Primer3: not found at this Tm N.d. 0.36
mm4__chr19 49.37 Mbp_chr19_49374718_F TCGTCGGCAGCGTCCCTTGTTCTTCCTCCCCAGG 59.6 0.36
mm4__chr19 49.37 Mbp_chr19_49374718_R GTCTCGTGGGCTCGGGGGGATGGAGTTCTGTGTGG 60.0 0.36
mm4__chr1 181.38 Mbp_chr1_181375729_F Primer3: not found at this Tm N.d. 0.35
mm4__chr1 181.38 Mbp_chr1_181375729_R Primer3: not found at this Tm N.d. 0.35
mm4__chr3 32.89 Mbp_chr3_32892099_F Primer3: not found at this Tm N.d. 0.35
mm4__chr3 32.89 Mbp_chr3_32892099_R Primer3: not found at this Tm N.d. 0.35
mm4__chr10 79.18 Mbp_chr10_79179097_F TCGTCGGCAGCGTCAGCTGTGACTGCTGACTCAC 59.9 0.35
mm4__chr10 79.18 Mbp_chr10_79179097_R GTCTCGTGGGCTCGGCTTGGAGGGCTGGCACATAC 60.7 0.35
mm4__chr10 58.84 Mbp_chr10_58837539_F TCGTCGGCAGCGTCTAATTGGGAGGTGAGGGGCA 60.5 0.35
mm4__chr10 58.84 Mbp_chr10_58837539_R GTCTCGTGGGCTCGGGTTCAGCTCTGGTCACAGCT 59.9 0.35
mm4__chr16 89.29 Mbp_chr16_89287089_F Primer3: not found at this Tm N.d. 0.35
mm4__chr16 89.29 Mbp_chr16_89287089_R Primer3: not found at this Tm N.d. 0.35
mm4__chr6 16.08 Mbp_chr6_16078009_F TCGTCGGCAGCGTCTACAGCCCAATGAAGCCCAA 59.5 0.35
mm4__chr6 16.08 Mbp_chr6_16078009_R GTCTCGTGGGCTCGGACCAGGACTTCGTTTGTGTC 58.0 0.35
mm4__chr20 44.93 Mbp_chr20_44931091_F Primer3: not found at this Tm N.d. 0.34
mm4__chr20 44.93 Mbp_chr20_44931091_R Primer3: not found at this Tm N.d. 0.34
mm4__chr5 168.05 Mbp_chr5_168047124_F TCGTCGGCAGCGTCAGCAACTGTGTCCAGAGTGT 59.1 0.34
mm4__chr5 168.05 Mbp_chr5_168047124_R GTCTCGTGGGCTCGGAGCCCAGATCTTCCAAACGG 60.0 0.34
mm4__chr5 15.45 Mbp_chr5_15446234_F TCGTCGGCAGCGTCTCATTCTTCAGGTGGCAGCG 60.6 0.34
mm4__chr5 15.45 Mbp_chr5_15446234_R GTCTCGTGGGCTCGGTCAGTGAGACCAAAGCGGAC 59.9 0.34
mm4__chr10 116.08 Mbp_chr10_116082380_F TCGTCGGCAGCGTCTTCCTTCTTGGCCTGGTGTC 59.8 0.34
mm4__chr10 116.08 Mbp_chr10_116082380_R GTCTCGTGGGCTCGGGGGCGGTTTGAGCTGAATGA 60.9 0.34
mm3__chr19 30.69 Mbp_chr19_30685762_F TCGTCGGCAGCGTCTAAAGAAATGGCCCCGTGCA 60.2 0.33
mm3__chr19 30.69 Mbp_chr19_30685762_R GTCTCGTGGGCTCGGGATCTCAGCAACGTGGCAGG 61.3 0.33
mm4__chr10 75.68 Mbp_chr10_75680659_F Primer3: not found at this Tm N.d. 0.33
mm4__chr10 75.68 Mbp_chr10_75680659_R Primer3: not found at this Tm N.d. 0.33
mm4__chr20 42.78 Mbp_chr20_42782235_F TCGTCGGCAGCGTCTGTTCAATGCCTGGGAGGTC 59.9 0.33
mm4__chr20 42.78 Mbp_chr20_42782235_R GTCTCGTGGGCTCGGGCAGCTGTTGAAGGGAGAGT 59.9 0.33
mm3__chr10 71.74 Mbp_chr10_71742343_F TCGTCGGCAGCGTCCAGAAAGGGTGCCTCTCTGG 60.0 0.33
mm3__chr10 71.74 Mbp_chr10_71742343_R GTCTCGTGGGCTCGGACCCTGACGTGTTGTGACAG 60.1 0.33
mm3__chr3 149.42 Mbp_chr3_149421159_F TCGTCGGCAGCGTCTCTTTTCTCAACAGTGATCCTACCT 59.4 0.32
mm3__chr3 149.42 Mbp_chr3_149421159_R GTCTCGTGGGCTCGGTTGTGACCTCCTCTCCTCCC 60.2 0.32
mm4__chr15 67.30 Mbp_chr15_67297574_F Primer3: not found at this Tm N.d. 0.32
mm4__chr15 67.30 Mbp_chr15_67297574_R Primer3: not found at this Tm N.d. 0.32
mm4__chr15 67.29 Mbp_chr15_67294854_F Primer3: not found at this Tm N.d. 0.32
mm4__chr15 67.29 Mbp_chr15_67294854_R Primer3: not found at this Tm N.d. 0.32
mm4__chr8 23.09 Mbp_chr8_23092619_F Primer3: not found at this Tm N.d. 0.31
mm4__chr8 23.09 Mbp_chr8_23092619_R Primer3: not found at this Tm N.d. 0.31
mm4__chr11 2.41 Mbp_chr11_2412145_F TCGTCGGCAGCGTCACCTGAGTGGCTTCATCTGG 59.3 0.31
mm4__chr11 2.41 Mbp_chr11_2412145_R GTCTCGTGGGCTCGGGGGGACAACTGGAACAAGTG 58.6 0.31
mm4__chrX 148.42 Mbp_chrX_148421937_F Primer3: not found at this Tm N.d. 0.31
mm4__chrX 148.42 Mbp_chrX_148421937_R Primer3: not found at this Tm N.d. 0.31
mm3__chr6 46.21 Mbp_chr6_46205060_F TCGTCGGCAGCGTCCGGCATGTGAAAAGCAGCTT 60.0 0.31
mm3__chr6 46.21 Mbp_chr6_46205060_R GTCTCGTGGGCTCGGCACGTAGGAGTCCTTCTGGC 59.8 0.31
mm4__chr1 57.90 Mbp_chr1_57896245_F Primer3: not found at this Tm N.d. 0.31
mm4__chr1 57.90 Mbp_chr1_57896245_R Primer3: not found at this Tm N.d. 0.31
mm4__chr22 46.32 Mbp_chr22_46317473_F Primer3: not found at this Tm N.d. 0.31
mm4__chr22 46.32 Mbp_chr22_46317473_R Primer3: not found at this Tm N.d. 0.31
mm4__chr12 45.93 Mbp_chr12_45926790_F Primer3: not found at this Tm N.d. 0.30
mm4__chr12 45.93 Mbp_chr12_45926790_R Primer3: not found at this Tm N.d. 0.30
mm4__chr1 68.12 Mbp_chr1_68123474_F TCGTCGGCAGCGTCTGTCTGGCTCACCACTCTTG 59.6 0.30
mm4__chr1 68.12 Mbp_chr1_68123474_R GTCTCGTGGGCTCGGCTGAGGGGTCCAGCAGATTG 60.1 0.30
mm4__chr2 100.97 Mbp_chr2_100969042_F TCGTCGGCAGCGTCTAGATGCTGCCTTTGTGGGG 60.0 0.30
mm4__chr2 100.97 Mbp_chr2_100969042_R GTCTCGTGGGCTCGGCCTTCTCCAAGGCTCCTCTG 59.4 0.30
mm1__chr3 175.98 Mbp_chr3_175978471_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 0.30
mm1__chr3 175.98 Mbp_chr3_175978471_R GTCTCGTGGGCTCGGGCAAAGGCTCCTGTATGTGC 59.5 0.30
mm4__chr12 123.41 Mbp_chr12_123407476_F TCGTCGGCAGCGTCACCAGAGCTGAGCACAAGTG 60.2 0.29
mm4__chr12 123.41 Mbp_chr12_123407476_R GTCTCGTGGGCTCGGTGATCAGTCTTCCTAGGCGA 57.5 0.29
mm4__chr17 79.17 Mbp_chr17_79167319_F Primer3: not found at this Tm N.d. 0.28
mm4__chr17 79.17 Mbp_chr17_79167319_R Primer3: not found at this Tm N.d. 0.28
mm4__chr21 36.49 Mbp_chr21_36493842_F TCGTCGGCAGCGTCGAACACATGTGGCTGGAGGA 59.9 0.28
mm4__chr21 36.49 Mbp_chr21_36493842_R GTCTCGTGGGCTCGGTGCCCAGCCACTCTATACCT 60.0 0.28
mm4__chrY 18.15 Mbp_chrY_18147968_F TCGTCGGCAGCGTCACAATGGCTCTTGCATGTGC 59.7 0.27
mm4__chrY 18.15 Mbp_chrY_18147968_R GTCTCGTGGGCTCGGGAGTCCTTCTGGCCCATGTC 60.1 0.27
mm4__chrY 17.76 Mbp_chrY_17758017_F TCGTCGGCAGCGTCGAGTCCTTCTGGCCCATGTC 60.1 0.27
mm4__chrY 17.76 Mbp_chrY_17758017_R GTCTCGTGGGCTCGGACAATGGCTCTTGCATGTGC 59.7 0.27
mm4__chr10 112.22 Mbp_chr10_112218299_F TCGTCGGCAGCGTCGATGCCATACAGAGCAGGGG 60.2 0.27
mm4__chr10 112.22 Mbp_chr10_112218299_R GTCTCGTGGGCTCGGCTTGGCGAGCCTTCAGTGAA 60.6 0.27
mm4__chr8 10.70 Mbp_chr8_10698402_F TCGTCGGCAGCGTCCAGCTTTGGAACTGCTGCAG 60.0 0.27
mm4__chr8 10.70 Mbp_chr8_10698402_R GTCTCGTGGGCTCGGGTCACATGACCCTCCCCAAG 60.0 0.27
mm3__chr12 89.12 Mbp_chr12_89123196_F TCGTCGGCAGCGTCACCAGATGAATATGTTAAATGCACC 57.9 0.26
mm3__chr12 89.12 Mbp_chr12_89123196_R GTCTCGTGGGCTCGGGCATGTACACCCAAGAAAAGCA 59.7 0.26
mm3__chr5 14.27 Mbp_chr5_14268293_F TCGTCGGCAGCGTCTGGCCTGGAATATGACAGCC 59.8 0.26
mm3__chr5 14.27 Mbp_chr5_14268293_R GTCTCGTGGGCTCGGAGAGCTGTGTGACCTGAGTC 59.0 0.26
mm4__chr15 99.75 Mbp_chr15_99750717_F Primer3: not found at this Tm N.d. 0.26
mm4__chr15 99.75 Mbp_chr15_99750717_R Primer3: not found at this Tm N.d. 0.26
mm4__chr15 99.75 Mbp_chr15_99750670_F Primer3: not found at this Tm N.d. 0.26
mm4__chr15 99.75 Mbp_chr15_99750670_R Primer3: not found at this Tm N.d. 0.26
mm4__chr15 99.75 Mbp_chr15_99750623_F Primer3: not found at this Tm N.d. 0.26
mm4__chr15 99.75 Mbp_chr15_99750623_R Primer3: not found at this Tm N.d. 0.26
mm4__chr18 65.75 Mbp_chr18_65750735_F Primer3: not found at this Tm N.d. 0.26
mm4__chr18 65.75 Mbp_chr18_65750735_R Primer3: not found at this Tm N.d. 0.26
mm4__chr12 114.25 Mbp_chr12_114245200_F TCGTCGGCAGCGTCGTTACCTCCCTGGCATCTGC 60.4 0.26
mm4__chr12 114.25 Mbp_chr12_114245200_R GTCTCGTGGGCTCGGGGGGTCACTGGCATTTATCCA 60.0 0.26
mm4__chr19 10.40 Mbp_chr19_10404842_F TCGTCGGCAGCGTCGCTTCATTCAAACCCGCGAG 60.1 0.25
mm4__chr19 10.40 Mbp_chr19_10404842_R GTCTCGTGGGCTCGGGGCCACTGATGTGCTGGAC 61.0 0.25
mm4__chr1 202.87 Mbp_chr1_202873376_F TCGTCGGCAGCGTCTGCTGGGTATAGTTCCCCGA 60.0 0.25
mm4__chr1 202.87 Mbp_chr1_202873376_R GTCTCGTGGGCTCGGGGAGTCCTTCTGGCCTATGC 59.8 0.25
mm4__chr12 121.44 Mbp_chr12_121442965_F TCGTCGGCAGCGTCGTGCAGCTCTAACCGCTCA 60.0 0.24
mm4__chr12 121.44 Mbp_chr12_121442965_R GTCTCGTGGGCTCGGTGACAGCTCAAACCTGGCAA 60.1 0.24
mm1__chr22 15.72 Mbp_chr22_15721571_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr22 15.72 Mbp_chr22_15721571_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.23
mm1__chr2 131.26 Mbp_chr2_131263640_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr2 131.26 Mbp_chr2_131263640_R GTCTCGTGGGCTCGGCTCTTTCTGATGCATGCCCC 58.9 0.23
mm1__chr2 130.66 Mbp_chr2_130656834_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr2 130.66 Mbp_chr2_130656834_R GTCTCGTGGGCTCGGCTCTTTCTGATGCATGCCCC 58.9 0.23
mm1__chr2 130.46 Mbp_chr2_130463835_F TCGTCGGCAGCGTCCTCTTTCTGATGCATGCCCC 58.9 0.23
mm1__chr2 130.46 Mbp_chr2_130463835_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr2 130.08 Mbp_chr2_130075263_F TCGTCGGCAGCGTCCTCTTTCTGATGCATGCCCC 58.9 0.23
mm1__chr2 130.08 Mbp_chr2_130075263_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr14 19.40 Mbp_chr14_19402800_F TCGTCGGCAGCGTCATCATGTTCTGGTGCCTGGG 60.0 0.23
mm1__chr14 19.40 Mbp_chr14_19402800_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr14 19.00 Mbp_chr14_18998952_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.23
mm1__chr14 19.00 Mbp_chr14_18998952_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.23
mm2__chr2 73.90 Mbp_chr2_73901399_F TCGTCGGCAGCGTCAGGCTTCGCAGGAGATGATG 59.8 0.23
mm2__chr2 73.90 Mbp_chr2_73901399_R GTCTCGTGGGCTCGGTATCCAGATGAGCACGCACC 59.8 0.23
mm4__chr19 27.79 Mbp_chr19_27786349_F Primer3: not found at this Tm N.d. 0.23
mm4__chr19 27.79 Mbp_chr19_27786349_R Primer3: not found at this Tm N.d. 0.23
mm4__chr5 179.78 Mbp_chr5_179776594_F TCGTCGGCAGCGTCCACCTTCCCTACCTTTCCCA 58.6 0.22
mm4__chr5 179.78 Mbp_chr5_179776594_R GTCTCGTGGGCTCGGGAGAAAAGCCGCTGAGGTCA 60.3 0.22
mm4__chr1 197.92 Mbp_chr1_197917717_F Primer3: not found at this Tm N.d. 0.22
mm4__chr1 197.92 Mbp_chr1_197917717_R Primer3: not found at this Tm N.d. 0.22
mm4__chr11 123.75 Mbp_chr11_123754200_F TCGTCGGCAGCGTCGTGGTGAAGGGGATTGCAGA 59.9 0.22
mm4__chr11 123.75 Mbp_chr11_123754200_R GTCTCGTGGGCTCGGATGTCCTGCTGCCTGCTG 60.0 0.22
mm4__chr2 95.06 Mbp_chr2_95056059_F Primer3: not found at this Tm N.d. 0.22
mm4__chr2 95.06 Mbp_chr2_95056059_R Primer3: not found at this Tm N.d. 0.22
mm3__chr16 50.06 Mbp_chr16_50059123_F TCGTCGGCAGCGTCCGCCCACATAGGAGTCCTTC 59.8 0.22
mm3__chr16 50.06 Mbp_chr16_50059123_R GTCTCGTGGGCTCGGTGGCTTTGCTAGGGACGATG 60.1 0.22
mm2__chr2 131.63 Mbp_chr2_131626223_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.22
mm2__chr2 131.63 Mbp_chr2_131626223_R GTCTCGTGGGCTCGGACTCCTTCTGATGCATGCCC 60.1 0.22
mm4__chr18 75.16 Mbp_chr18_75157554_F Primer3: not found at this Tm N.d. 0.22
mm4__chr18 75.16 Mbp_chr18_75157554_R Primer3: not found at this Tm N.d. 0.22
mm4__chr11 27.90 Mbp_chr11_27898844_F Primer3: not found at this Tm N.d. 0.22
mm4__chr11 27.90 Mbp_chr11_27898844_R Primer3: not found at this Tm N.d. 0.22
mm4__chr6 158.65 Mbp_chr6_158649010_F Primer3: not found at this Tm N.d. 0.22
mm4__chr6 158.65 Mbp_chr6_158649010_R Primer3: not found at this Tm N.d. 0.22
mm4__chr22 19.92 Mbp_chr22_19924816_F Primer3: not found at this Tm N.d. 0.21
mm4__chr22 19.92 Mbp_chr22_19924816_R Primer3: not found at this Tm N.d. 0.21
mm4__chr22 17.02 Mbp_chr22_17015514_F TCGTCGGCAGCGTCATGCTCAAAAGGAAGGGCCA 59.8 0.21
mm4__chr22 17.02 Mbp_chr22_17015514_R GTCTCGTGGGCTCGGTCCTCTGCAGGACCTTCTCT 59.5 0.21
mm4__chr22 11.02 Mbp_chr22_11017397_F TCGTCGGCAGCGTCTCCTCTGCAGGACCTTCTCT 59.5 0.21
mm4__chr22 11.02 Mbp_chr22_11017397_R GTCTCGTGGGCTCGGGAGTCCCCTGAACGCTCAAA 59.9 0.21
mm4__chr21 10.36 Mbp_chr21_10364477_F TCGTCGGCAGCGTCTCCTCTGCAGGACCTTCTCT 59.5 0.21
mm4__chr21 10.36 Mbp_chr21_10364477_R GTCTCGTGGGCTCGGGAGTCCCCTGAACGCTCAAA 59.9 0.21
mm4__chr5 73.30 Mbp_chr5_73297450_F TCGTCGGCAGCGTCAGGAAAGAAGCGGGGTAAGC 60.0 0.21
mm4__chr5 73.30 Mbp_chr5_73297450_R GTCTCGTGGGCTCGGAACAGAGGCTCCCAATTCCC 59.6 0.21
mm4__chr2 241.32 Mbp_chr2_241315785_F TCGTCGGCAGCGTCCAACTGCCGTGGGAGGAG 60.0 0.21
mm4__chr2 241.32 Mbp_chr2_241315785_R GTCTCGTGGGCTCGGAACTGCTTCCTGGCTCAGTG 60.2 0.21
mm4__chr17 81.51 Mbp_chr17_81512246_F TCGTCGGCAGCGTCCCATCCACTCACCTGGTGTC 60.0 0.20
mm4__chr17 81.51 Mbp_chr17_81512246_R GTCTCGTGGGCTCGGATGGAAGAAGAGATCGCCGC 60.2 0.20
mm4__chr3 165.68 Mbp_chr3_165675618_F Primer3: not found at this Tm N.d. 0.20
mm4__chr3 165.68 Mbp_chr3_165675618_R Primer3: not found at this Tm N.d. 0.20
mm4__chr17 47.85 Mbp_chr17_47849274_F TCGTCGGCAGCGTCCTCATACCAGTGCCAGGCAA 60.0 0.20
mm4__chr17 47.85 Mbp_chr17_47849274_R GTCTCGTGGGCTCGGGAAGCCATTCCCTCCCAACT 59.6 0.20
mm4__chr9 131.53 Mbp_chr9_131530171_F TCGTCGGCAGCGTCGTGTGTGGAGGGCCCTAAAG 60.3 0.20
mm4__chr9 131.53 Mbp_chr9_131530171_R GTCTCGTGGGCTCGGAGGAAGCCTGTTGCACTGTG 60.8 0.20
mm3__chr12 123.40 Mbp_chr12_123402769_F Primer3: not found at this Tm N.d. 0.20
mm3__chr12 123.40 Mbp_chr12_123402769_R Primer3: not found at this Tm N.d. 0.20
mm3__chr7 68.00 Mbp_chr7_67998279_F TCGTCGGCAGCGTCTCCAATCCCAACCAAACCGT 59.8 0.20
mm3__chr7 68.00 Mbp_chr7_67998279_R GTCTCGTGGGCTCGGACCTCTCACCTGTAGCCTGA 59.5 0.20
mm4__chr15 101.16 Mbp_chr15_101163245_F TCGTCGGCAGCGTCGCACCAAACCGGCTCTATCT 60.1 0.20
mm4__chr15 101.16 Mbp_chr15_101163245_R GTCTCGTGGGCTCGGCCCTGCCTCTGCTACCATTC 60.1 0.20
mm4__chr18 71.72 Mbp_chr18_71715752_F Primer3: not found at this Tm N.d. 0.20
mm4__chr18 71.72 Mbp_chr18_71715752_R Primer3: not found at this Tm N.d. 0.20
mm4__chr7 76.36 Mbp_chr7_76359847_F TCGTCGGCAGCGTCACGAACGACAAATACCGGCT 60.0 0.20
mm4__chr7 76.36 Mbp_chr7_76359847_R GTCTCGTGGGCTCGGGGCTCCTGGTAGCTGAACTC 59.8 0.20
mm4__chr19 55.34 Mbp_chr19_55343509_F TCGTCGGCAGCGTCAGTAGAAGGGTCCTGTGGGG 60.2 0.19
mm4__chr19 55.34 Mbp_chr19_55343509_R GTCTCGTGGGCTCGGGCTGGCTATTGGCGATCCAT 60.6 0.19
mm4__chr7 81.43 Mbp_chr7_81430853_F Primer3: not found at this Tm N.d. 0.19
mm4__chr7 81.43 Mbp_chr7_81430853_R Primer3: not found at this Tm N.d. 0.19
mm4__chr8 104.73 Mbp_chr8_104732280_F TCGTCGGCAGCGTCGCACGGAGGTGGTGTTTACT 60.2 0.19
mm4__chr8 104.73 Mbp_chr8_104732280_R GTCTCGTGGGCTCGGCAGCCAACTCTGCCTCCAAA 60.5 0.19
mm4__chr4 33.59 Mbp_chr4_33593011_F Primer3: not found at this Tm N.d. 0.19
mm4__chr4 33.59 Mbp_chr4_33593011_R Primer3: not found at this Tm N.d. 0.19
mm4__chr4 183.29 Mbp_chr4_183289315_F Primer3: not found at this Tm N.d. 0.19
mm4__chr4 183.29 Mbp_chr4_183289315_R Primer3: not found at this Tm N.d. 0.19
mm4__chr8 32.69 Mbp_chr8_32692480_F Primer3: not found at this Tm N.d. 0.19
mm4__chr8 32.69 Mbp_chr8_32692480_R Primer3: not found at this Tm N.d. 0.19
mm2__chr2 27.37 Mbp_chr2_27373995_F TCGTCGGCAGCGTCAGGATGTCCTGGAGGTGAGG 60.3 0.19
mm2__chr2 27.37 Mbp_chr2_27373995_R GTCTCGTGGGCTCGGCAGCTACAGCCTGGGGATAG 59.3 0.19
mm4__chr17 39.08 Mbp_chr17_39083401_F Primer3: not found at this Tm N.d. 0.19
mm4__chr17 39.08 Mbp_chr17_39083401_R Primer3: not found at this Tm N.d. 0.19
mm4__chr8 70.40 Mbp_chr8_70402448_F TCGTCGGCAGCGTCGCTCCAGTCCCCGATTCC 59.4 0.18
mm4__chr8 70.40 Mbp_chr8_70402448_R GTCTCGTGGGCTCGGGTCGGTCCCTGGTTAGATGG 59.5 0.18
mm4__chr1 105.86 Mbp_chr1_105862469_F TCGTCGGCAGCGTCAGGTAAACGCCACAGGAAGG 59.9 0.18
mm4__chr1 105.86 Mbp_chr1_105862469_R GTCTCGTGGGCTCGGTGGAATCTGCCACATGACAGA 59.3 0.18
mm4__chr3 47.84 Mbp_chr3_47840860_F TCGTCGGCAGCGTCAGGCCGACTGAGGTGTAAAG 59.3 0.17
mm4__chr3 47.84 Mbp_chr3_47840860_R GTCTCGTGGGCTCGGTCGGTATTTGGCTGCGTCAA 60.3 0.17
mm3__chr11 54.56 Mbp_chr11_54558482_F Primer3: not found at this Tm N.d. 0.17
mm3__chr11 54.56 Mbp_chr11_54558482_R Primer3: not found at this Tm N.d. 0.17
mm3__chr11 48.38 Mbp_chr11_48384228_F Primer3: not found at this Tm N.d. 0.17
mm3__chr11 48.38 Mbp_chr11_48384228_R Primer3: not found at this Tm N.d. 0.17
mm3__chr1 229.43 Mbp_chr1_229433001_F TCGTCGGCAGCGTCGCAGCCTGACCTGGTGTC 60.3 0.17
mm3__chr1 229.43 Mbp_chr1_229433001_R GTCTCGTGGGCTCGGGACGAAGACGAGACCACCG 60.1 0.17
mm4__chr7 27.10 Mbp_chr7_27102193_F Primer3: not found at this Tm N.d. 0.17
mm4__chr7 27.10 Mbp_chr7_27102193_R Primer3: not found at this Tm N.d. 0.17
mm4__chr20 49.81 Mbp_chr20_49812915_F Primer3: not found at this Tm N.d. 0.17
mm4__chr20 49.81 Mbp_chr20_49812915_R Primer3: not found at this Tm N.d. 0.17
mm3__chr1 162.02 Mbp_chr1_162019365_F Primer3: not found at this Tm N.d. 0.17
mm3__chr1 162.02 Mbp_chr1_162019365_R Primer3: not found at this Tm N.d. 0.17
mm3__chr1 215.08 Mbp_chr1_215081844_F TCGTCGGCAGCGTCTCGCCTATTACGACGACAGC 59.9 0.16
mm3__chr1 215.08 Mbp_chr1_215081844_R GTCTCGTGGGCTCGGCGTAGGGACAGCCAAACTCG 60.7 0.16
mm4__chr1 52.91 Mbp_chr1_52910153_F Primer3: not found at this Tm N.d. 0.16
mm4__chr1 52.91 Mbp_chr1_52910153_R Primer3: not found at this Tm N.d. 0.16
mm3__chr4 5.82 Mbp_chr4_5816710_F Primer3: not found at this Tm N.d. 0.16
mm3__chr4 5.82 Mbp_chr4_5816710_R Primer3: not found at this Tm N.d. 0.16
mm4__chrX 125.57 Mbp_chrX_125567179_F Primer3: not found at this Tm N.d. 0.16
mm4__chrX 125.57 Mbp_chrX_125567179_R Primer3: not found at this Tm N.d. 0.16
mm3__chr10 122.46 Mbp_chr10_122459472_F Primer3: not found at this Tm N.d. 0.15
mm3__chr10 122.46 Mbp_chr10_122459472_R Primer3: not found at this Tm N.d. 0.15
mm4__chr12 118.04 Mbp_chr12_118039557_F TCGTCGGCAGCGTCATGCAAACGTGTGTGCACAG 60.2 0.15
mm4__chr12 118.04 Mbp_chr12_118039557_R GTCTCGTGGGCTCGGTCTCTCTCTTGCCCTCCCTG 60.3 0.15
mm4__chr4 45.03 Mbp_chr4_45034172_F TCGTCGGCAGCGTCGAGGACAAGGAGGGCCATTC 60.1 0.15
mm4__chr4 45.03 Mbp_chr4_45034172_R GTCTCGTGGGCTCGGGCAGACAGACTCCCCTAAGG 59.1 0.15
mm3__chr1 66.76 Mbp_chr1_66756775_F TCGTCGGCAGCGTCACAAAGTCCACAGACCCCTA 57.8 0.15
mm3__chr1 66.76 Mbp_chr1_66756775_R GTCTCGTGGGCTCGGGAAGCAACATGGGGCAATGG 60.1 0.15
mm4__chr19 9.94 Mbp_chr19_9935325_F Primer3: not found at this Tm N.d. 0.15
mm4__chr19 9.94 Mbp_chr19_9935325_R Primer3: not found at this Tm N.d. 0.15
mm3__chr19 35.53 Mbp_chr19_35534553_F TCGTCGGCAGCGTCCCCCAGGCTTTTCTCCAACC 60.9 0.15
mm3__chr19 35.53 Mbp_chr19_35534553_R GTCTCGTGGGCTCGGCAGATGGTCCTCCTCTCCCA 60.0 0.15
mm3__chr6 33.66 Mbp_chr6_33662727_F TCGTCGGCAGCGTCGAGGTCAGAAAAGCCCTGCT 59.9 0.15
mm3__chr6 33.66 Mbp_chr6_33662727_R GTCTCGTGGGCTCGGCAGAGGGAGGTAGGGGTCAA 59.9 0.15
mm3__chr8 96.32 Mbp_chr8_96322385_F Primer3: not found at this Tm N.d. 0.15
mm3__chr8 96.32 Mbp_chr8_96322385_R Primer3: not found at this Tm N.d. 0.15
mm4__chr6 44.13 Mbp_chr6_44133255_F TCGTCGGCAGCGTCCTTTGCAGAGACTCCCTGGG 60.0 0.14
mm4__chr6 44.13 Mbp_chr6_44133255_R GTCTCGTGGGCTCGGAACACTTCAACCACAGGGCA 60.0 0.14
mm4__chr7 4.30 Mbp_chr7_4298332_F TCGTCGGCAGCGTCGAGAGTAAAGCGGTGCCCAT 60.1 0.14
mm4__chr7 4.30 Mbp_chr7_4298332_R GTCTCGTGGGCTCGGAGCACTATCAGAGCCAGCAG 59.5 0.14
mm4__chr16 19.17 Mbp_chr16_19174521_F TCGTCGGCAGCGTCGTTTCAGCTCAGTCCTTGGC 59.1 0.14
mm4__chr16 19.17 Mbp_chr16_19174521_R GTCTCGTGGGCTCGGAGAGAAAATGAGAGGCCTGAGG 59.4 0.14
mm3__chr20 62.59 Mbp_chr20_62590954_F TCGTCGGCAGCGTCATTCTGGTTCCCTGAGCAGC 60.0 0.14
mm3__chr20 62.59 Mbp_chr20_62590954_R GTCTCGTGGGCTCGGGCAATGCACTTGGGGACATG 60.1 0.14
mm4__chr1 46.44 Mbp_chr1_46442706_F Primer3: not found at this Tm N.d. 0.14
mm4__chr1 46.44 Mbp_chr1_46442706_R Primer3: not found at this Tm N.d. 0.14
mm4__chr12 116.53 Mbp_chr12_116534154_F TCGTCGGCAGCGTCTCCGGTTACAACTAGACGCG 59.8 0.14
mm4__chr12 116.53 Mbp_chr12_116534154_R GTCTCGTGGGCTCGGTTGGGAAGGCACAGAGAACG 60.2 0.14
mm4__chr14 95.91 Mbp_chr14_95905346_F Primer3: not found at this Tm N.d. 0.14
mm4__chr14 95.91 Mbp_chr14_95905346_R Primer3: not found at this Tm N.d. 0.14
mm4__chr2 18.05 Mbp_chr2_18053903_F Primer3: not found at this Tm N.d. 0.14
mm4__chr2 18.05 Mbp_chr2_18053903_R Primer3: not found at this Tm N.d. 0.14
mm4__chr5 54.59 Mbp_chr5_54586524_F Primer3: not found at this Tm N.d. 0.14
mm4__chr5 54.59 Mbp_chr5_54586524_R Primer3: not found at this Tm N.d. 0.14
mm4__chr5 6.18 Mbp_chr5_6180963_F TCGTCGGCAGCGTCGTGGTGCTCCCTCCTCTTTC 60.0 0.13
mm4__chr5 6.18 Mbp_chr5_6180963_R GTCTCGTGGGCTCGGGGGGAGCACTACAGGAGAGA 60.0 0.13
mm3__chr5 6.18 Mbp_chr5_6181269_F Primer3: not found at this Tm N.d. 0.13
mm3__chr5 6.18 Mbp_chr5_6181269_R Primer3: not found at this Tm N.d. 0.13
mm3__chr5 6.18 Mbp_chr5_6180838_F TCGTCGGCAGCGTCGTGACCCTCCTCTCCCTCTC 60.3 0.13
mm3__chr5 6.18 Mbp_chr5_6180838_R GTCTCGTGGGCTCGGGAAAGAGGAGGGAGCACCAC 60.0 0.13
mm3__chr5 6.18 Mbp_chr5_6180769_F TCGTCGGCAGCGTCGTGACCCTCCTCTCCCTCTC 60.3 0.13
mm3__chr5 6.18 Mbp_chr5_6180769_R GTCTCGTGGGCTCGGAGGCTCACACCTCCCTCAG 60.6 0.13
mm3__chr5 6.18 Mbp_chr5_6180592_F TCGTCGGCAGCGTCAGGTGTGACCCTCCCTTCTT 60.1 0.13
mm3__chr5 6.18 Mbp_chr5_6180592_R GTCTCGTGGGCTCGGCATCAGGATGGAGGGGAGGA 60.1 0.13
mm3__chr17 3.92 Mbp_chr17_3915975_F TCGTCGGCAGCGTCCCTCGTCCCCATCTCAACAG 59.8 0.13
mm3__chr17 3.92 Mbp_chr17_3915975_R GTCTCGTGGGCTCGGTCTGACATCCCTTGCGTGAC 60.0 0.13
mm4__chr2 71.83 Mbp_chr2_71827637_F TCGTCGGCAGCGTCGCTGGGGTTTCTGGGAGTTT 60.1 0.13
mm4__chr2 71.83 Mbp_chr2_71827637_R GTCTCGTGGGCTCGGGAACTCTGGCACCTTCCCAA 59.8 0.13
mm4__chr3 143.86 Mbp_chr3_143855297_F TCGTCGGCAGCGTCGGAAAGCCGAGGACAGCATA 59.8 0.13
mm4__chr3 143.86 Mbp_chr3_143855297_R GTCTCGTGGGCTCGGCTGCTGTCTCTGCAAAAGGC 59.7 0.13
mm4__chr15 62.59 Mbp_chr15_62591612_F TCGTCGGCAGCGTCTGAGTCACACCATGGTCAAGG 59.9 0.13
mm4__chr15 62.59 Mbp_chr15_62591612_R GTCTCGTGGGCTCGGAGATCACATCTGGCCAGAGG 58.8 0.13
mm4__chr17 57.12 Mbp_chr17_57121995_F TCGTCGGCAGCGTCTGAGCTGTGGCCATTGTCAG 60.6 0.13
mm4__chr17 57.12 Mbp_chr17_57121995_R GTCTCGTGGGCTCGGGCAGAGGGCATTTCCATGAT 58.2 0.13
mm4__chr21 32.42 Mbp_chr21_32424051_F Primer3: not found at this Tm N.d. 0.13
mm4__chr21 32.42 Mbp_chr21_32424051_R Primer3: not found at this Tm N.d. 0.13
mm4__chr16 74.40 Mbp_chr16_74397860_F TCGTCGGCAGCGTCGAAGGAAACCTCAGGTCGGG 60.0 0.13
mm4__chr16 74.40 Mbp_chr16_74397860_R GTCTCGTGGGCTCGGGGGGATCCCACTGCTTGG 59.7 0.13
mm4__chr3 12.07 Mbp_chr3_12070309_F TCGTCGGCAGCGTCTCATTGACAGTGTCTCCGGC 60.0 0.13
mm4__chr3 12.07 Mbp_chr3_12070309_R GTCTCGTGGGCTCGGGAGTCCTTCTGACCCATGCC 60.1 0.13
mm2__chr11 36.67 Mbp_chr11_36666776_F Primer3: not found at this Tm N.d. 0.13
mm2__chr11 36.67 Mbp_chr11_36666776_R Primer3: not found at this Tm N.d. 0.13
mm4__chr3 10.29 Mbp_chr3_10289185_F Primer3: not found at this Tm N.d. 0.12
mm4__chr3 10.29 Mbp_chr3_10289185_R Primer3: not found at this Tm N.d. 0.12
mm4__chr5 133.14 Mbp_chr5_133144675_F Primer3: not found at this Tm N.d. 0.12
mm4__chr5 133.14 Mbp_chr5_133144675_R Primer3: not found at this Tm N.d. 0.12
mm4__chr18 62.99 Mbp_chr18_62992885_F Primer3: not found at this Tm N.d. 0.12
mm4__chr18 62.99 Mbp_chr18_62992885_R Primer3: not found at this Tm N.d. 0.12
mm4__chr18 59.03 Mbp_chr18_59026393_F Primer3: not found at this Tm N.d. 0.12
mm4__chr18 59.03 Mbp_chr18_59026393_R Primer3: not found at this Tm N.d. 0.12
mm4__chr11 69.75 Mbp_chr11_69753548_F TCGTCGGCAGCGTCCCTGTGGTGGATCCTACGG 59.1 0.12
mm4__chr11 69.75 Mbp_chr11_69753548_R GTCTCGTGGGCTCGGGCACAGTCACTGCTCATGACA 60.8 0.12
mm4__chr2 100.10 Mbp_chr2_100104757_F Primer3: not found at this Tm N.d. 0.12
mm4__chr2 100.10 Mbp_chr2_100104757_R Primer3: not found at this Tm N.d. 0.12
mm4__chr19 12.99 Mbp_chr19_12985900_F TCGTCGGCAGCGTCGAGATCTGATGCCAGCTGGG 60.2 0.12
mm4__chr19 12.99 Mbp_chr19_12985900_R GTCTCGTGGGCTCGGGCTGCATCTAGAGGCCACTC 60.2 0.12
mm4__chr8 29.58 Mbp_chr8_29576466_F Primer3: not found at this Tm N.d. 0.12
mm4__chr8 29.58 Mbp_chr8_29576466_R Primer3: not found at this Tm N.d. 0.12
mm4__chr19 40.40 Mbp_chr19_40398532_F TCGTCGGCAGCGTCTGACAAGACAGAGGGCAAGG 59.6 0.12
mm4__chr19 40.40 Mbp_chr19_40398532_R GTCTCGTGGGCTCGGAAGCTGGTACGCGTGCTTAG 60.7 0.12
mm3__chr12 1.82 Mbp_chr12_1820195_F Primer3: not found at this Tm N.d. 0.12
mm3__chr12 1.82 Mbp_chr12_1820195_R Primer3: not found at this Tm N.d. 0.12
mm2__chr8 72.27 Mbp_chr8_72268160_F Primer3: not found at this Tm N.d. 0.11
mm2__chr8 72.27 Mbp_chr8_72268160_R Primer3: not found at this Tm N.d. 0.11
mm4__chr10 86.34 Mbp_chr10_86341339_F TCGTCGGCAGCGTCTGCACTGCCCACTAAACAGT 59.8 0.11
mm4__chr10 86.34 Mbp_chr10_86341339_R GTCTCGTGGGCTCGGTAAGTACCCAGGGTCCCACC 60.2 0.11
mm4__chr15 28.66 Mbp_chr15_28657610_F TCGTCGGCAGCGTCCACCCTCTTCTCCGTGTCAC 60.0 0.11
mm4__chr15 28.66 Mbp_chr15_28657610_R GTCTCGTGGGCTCGGCTGGATCCGGCAGAGAACAG 60.1 0.11
mm4__chr15 22.56 Mbp_chr15_22563372_F TCGTCGGCAGCGTCCACCCTCTTCTCCGTGTCAC 60.0 0.11
mm4__chr15 22.56 Mbp_chr15_22563372_R GTCTCGTGGGCTCGGCTGGATCCGGCAGAGAACAG 60.1 0.11
mm4__chr15 20.44 Mbp_chr15_20436985_F TCGTCGGCAGCGTCCTGGATCCGGCAGAGAACAG 60.1 0.11
mm4__chr15 20.44 Mbp_chr15_20436985_R GTCTCGTGGGCTCGGCACCCTCTTCTCCGTGTCAC 60.0 0.11
mm4__chr16 54.20 Mbp_chr16_54203201_F TCGTCGGCAGCGTCCCACTTCTTTGGGGAGTGCT 59.8 0.11
mm4__chr16 54.20 Mbp_chr16_54203201_R GTCTCGTGGGCTCGGGTTACTCAAGCCCTGGTGCT 59.9 0.11
mm3__chr16 26.92 Mbp_chr16_26917228_F TCGTCGGCAGCGTCAAAGTCCCCTGCCTTCCATG 59.9 0.11
mm3__chr16 26.92 Mbp_chr16_26917228_R GTCTCGTGGGCTCGGATGTGGCCATCACTTCCCTG 60.0 0.11
mm4__chr17 4.96 Mbp_chr17_4957641_F Primer3: not found at this Tm N.d. 0.11
mm4__chr17 4.96 Mbp_chr17_4957641_R Primer3: not found at this Tm N.d. 0.11
mm4__chr7 42.58 Mbp_chr7_42576775_F Primer3: not found at this Tm N.d. 0.11
mm4__chr7 42.58 Mbp_chr7_42576775_R Primer3: not found at this Tm N.d. 0.11
mm4__chr15 25.58 Mbp_chr15_25578724_F Primer3: not found at this Tm N.d. 0.11
mm4__chr15 25.58 Mbp_chr15_25578724_R Primer3: not found at this Tm N.d. 0.11
mm2__chr3 184.16 Mbp_chr3_184156562_F TCGTCGGCAGCGTCGCCAGTGGAGAGACGTTAGG 59.8 0.11
mm2__chr3 184.16 Mbp_chr3_184156562_R GTCTCGTGGGCTCGGTCCAAACTGTGCCTGTCCAG 60.1 0.11
mm3__chr19 54.14 Mbp_chr19_54141815_F TCGTCGGCAGCGTCTGACTACTCCAGGGTTCCCT 59.2 0.10
mm3__chr19 54.14 Mbp_chr19_54141815_R GTCTCGTGGGCTCGGAGACTTCTGAGATACCAGCGG 58.9 0.10
mm4__chr11 35.14 Mbp_chr11_35142768_F TCGTCGGCAGCGTCCAAGTCCTTGTGTGGGAGGC 60.8 0.10
mm4__chr11 35.14 Mbp_chr11_35142768_R GTCTCGTGGGCTCGGTGGAAGGGAATCTGTCATGAACT 59.4 0.10
mm4__chr15 40.36 Mbp_chr15_40357831_F TCGTCGGCAGCGTCCCCAGGTCCAAGTCGAAGTC 60.0 0.10
mm4__chr15 40.36 Mbp_chr15_40357831_R GTCTCGTGGGCTCGGCTTCAGGCCTGCGAGGTTC 60.7 0.10
mm4__chr7 36.57 Mbp_chr7_36572902_F Primer3: not found at this Tm N.d. 0.10
mm4__chr7 36.57 Mbp_chr7_36572902_R Primer3: not found at this Tm N.d. 0.10
mm4__chr9 81.82 Mbp_chr9_81822329_F TCGTCGGCAGCGTCTTTTAGCTGGAGGGTGAGCC 59.6 0.10
mm4__chr9 81.82 Mbp_chr9_81822329_R GTCTCGTGGGCTCGGTCACACAAAGCAGATTTCAGCA 59.0 0.10
mm4__chr5 6.18 Mbp_chr5_6180654_F TCGTCGGCAGCGTCCCCCTCTCCTTCTCTCATGC 59.2 0.10
mm4__chr5 6.18 Mbp_chr5_6180654_R GTCTCGTGGGCTCGGGAGAGAGGGAGAGGAGGGTC 59.8 0.10
mm4__chr5 153.86 Mbp_chr5_153856620_F Primer3: not found at this Tm N.d. 0.10
mm4__chr5 153.86 Mbp_chr5_153856620_R Primer3: not found at this Tm N.d. 0.10
mm2__chr15 34.79 Mbp_chr15_34794694_F TCGTCGGCAGCGTCGGGAAGTTTACCTGGTGCCG 60.9 0.10
mm2__chr15 34.79 Mbp_chr15_34794694_R GTCTCGTGGGCTCGGATGTGTGACGACGAGGAGAC 59.4 0.10
mm4__chr5 6.18 Mbp_chr5_6181207_F TCGTCGGCAGCGTCTCCTTCTCTCCTGTGGTGCT 60.1 0.09
mm4__chr5 6.18 Mbp_chr5_6181207_R GTCTCGTGGGCTCGGGGAGAGAGGAAAGGGCACAC 60.0 0.09
mm3__chr10 124.53 Mbp_chr10_124526823_F TCGTCGGCAGCGTCCCAGAAGTCCCATCGTTGCT 60.0 0.09
mm3__chr10 124.53 Mbp_chr10_124526823_R GTCTCGTGGGCTCGGGGCTTCCCAGATCTGCCTG 60.1 0.09
mm3__chrX 86.32 Mbp_chrX_86322419_F Primer3: not found at this Tm N.d. 0.09
mm3__chrX 86.32 Mbp_chrX_86322419_R Primer3: not found at this Tm N.d. 0.09
mm3__chr5 28.01 Mbp_chr5_28013333_F Primer3: not found at this Tm N.d. 0.09
mm3__chr5 28.01 Mbp_chr5_28013333_R Primer3: not found at this Tm N.d. 0.09
mm3__chr12 60.65 Mbp_chr12_60648399_F Primer3: not found at this Tm N.d. 0.09
mm3__chr12 60.65 Mbp_chr12_60648399_R Primer3: not found at this Tm N.d. 0.09
mm3__chr1 42.51 Mbp_chr1_42511088_F Primer3: not found at this Tm N.d. 0.09
mm3__chr1 42.51 Mbp_chr1_42511088_R Primer3: not found at this Tm N.d. 0.09
mm4__chr2 239.40 Mbp_chr2_239404451_F Primer3: not found at this Tm N.d. 0.09
mm4__chr2 239.40 Mbp_chr2_239404451_R Primer3: not found at this Tm N.d. 0.09
mm4__chr1 28.86 Mbp_chr1_28856882_F TCGTCGGCAGCGTCGGTTGGATGTGTGACCCTGT 59.8 0.09
mm4__chr1 28.86 Mbp_chr1_28856882_R GTCTCGTGGGCTCGGCATCCCCATGCAACTGGACT 60.0 0.09
mm4__chr6 151.53 Mbp_chr6_151529899_F Primer3: not found at this Tm N.d. 0.09
mm4__chr6 151.53 Mbp_chr6_151529899_R Primer3: not found at this Tm N.d. 0.09
mm4__chr4 151.58 Mbp_chr4_151577808_F TCGTCGGCAGCGTCTGCTGAGCTCTTGTTCAGGA 58.9 0.09
mm4__chr4 151.58 Mbp_chr4_151577808_R GTCTCGTGGGCTCGGGCGCCTTGGTCTTCACTAGT 60.0 0.09
mm3__chr4 2.00 Mbp_chr4_1996233_F Primer3: not found at this Tm N.d. 0.08
mm3__chr4 2.00 Mbp_chr4_1996233_R Primer3: not found at this Tm N.d. 0.08
mm4__chr11 77.12 Mbp_chr11_77120616_F Primer3: not found at this Tm N.d. 0.08
mm4__chr11 77.12 Mbp_chr11_77120616_R Primer3: not found at this Tm N.d. 0.08
mm4__chr3 75.85 Mbp_chr3_75853453_F Primer3: not found at this Tm N.d. 0.08
mm4__chr3 75.85 Mbp_chr3_75853453_R Primer3: not found at this Tm N.d. 0.08
mm3__chr8 43.43 Mbp_chr8_43431852_F Primer3: not found at this Tm N.d. 0.08
mm3__chr8 43.43 Mbp_chr8_43431852_R Primer3: not found at this Tm N.d. 0.08
mm4__chr15 88.26 Mbp_chr15_88256806_F TCGTCGGCAGCGTCGGGTTATCGGAGCGTCTTCC 60.2 0.08
mm4__chr15 88.26 Mbp_chr15_88256806_R GTCTCGTGGGCTCGGGAGATTCCGCTCTCCTGCC 59.9 0.08
mm4__chr2 236.77 Mbp_chr2_236767634_F Primer3: not found at this Tm N.d. 0.08
mm4__chr2 236.77 Mbp_chr2_236767634_R Primer3: not found at this Tm N.d. 0.08
mm3__chr11 131.15 Mbp_chr11_131149093_F Primer3: not found at this Tm N.d. 0.08
mm3__chr11 131.15 Mbp_chr11_131149093_R Primer3: not found at this Tm N.d. 0.08
mm4__chr5 6.18 Mbp_chr5_6181024_F Primer3: not found at this Tm N.d. 0.08
mm4__chr5 6.18 Mbp_chr5_6181024_R Primer3: not found at this Tm N.d. 0.08
mm4__chr15 69.39 Mbp_chr15_69393658_F TCGTCGGCAGCGTCCCTCTCCCTCCTGCTCCAT 60.0 0.08
mm4__chr15 69.39 Mbp_chr15_69393658_R GTCTCGTGGGCTCGGACTGAGGTTCCTGGTCTCCC 60.5 0.08
mm4__chr3 54.29 Mbp_chr3_54293973_F Primer3: not found at this Tm N.d. 0.08
mm4__chr3 54.29 Mbp_chr3_54293973_R Primer3: not found at this Tm N.d. 0.08
mm4__chr9 130.34 Mbp_chr9_130343907_F TCGTCGGCAGCGTCGCTACAGGGTTGGGACTGAG 59.7 0.08
mm4__chr9 130.34 Mbp_chr9_130343907_R GTCTCGTGGGCTCGGACCACACACACCACCAAGTG 60.6 0.08
mm4__chr17 42.56 Mbp_chr17_42558899_F Primer3: not found at this Tm N.d. 0.08
mm4__chr17 42.56 Mbp_chr17_42558899_R Primer3: not found at this Tm N.d. 0.08
mm3__chr1 246.53 Mbp_chr1_246530783_F Primer3: not found at this Tm N.d. 0.08
mm3__chr1 246.53 Mbp_chr1_246530783_R Primer3: not found at this Tm N.d. 0.08
mm4__chr4 80.18 Mbp_chr4_80184143_F TCGTCGGCAGCGTCCGGTACCCAACCCTCTCTCT 60.3 0.08
mm4__chr4 80.18 Mbp_chr4_80184143_R GTCTCGTGGGCTCGGGGTGCTCTGGTCCAGAAACT 59.6 0.08
mm4__chr1 158.08 Mbp_chr1_158079231_F TCGTCGGCAGCGTCGCCCCTCTTCCTTTCCTTCC 60.0 0.08
mm4__chr1 158.08 Mbp_chr1_158079231_R GTCTCGTGGGCTCGGACTCCCTCATCTCAGCTCCT 59.3 0.08
mm4__chr10 104.15 Mbp_chr10_104152797_F TCGTCGGCAGCGTCAGGCTCCTAGGAAGTGATGT 57.4 0.07
mm4__chr10 104.15 Mbp_chr10_104152797_R GTCTCGTGGGCTCGGCCAAGTGGGAGCATCTCTGG 60.1 0.07
mm4__chr9 131.48 Mbp_chr9_131482040_F TCGTCGGCAGCGTCGATTTCTGGCCTGTGCATGG 59.5 0.07
mm4__chr9 131.48 Mbp_chr9_131482040_R GTCTCGTGGGCTCGGCCAAACCAGACTACGTGGCT 59.9 0.07
mm4__chrX 72.02 Mbp_chrX_72017129_F TCGTCGGCAGCGTCAAACAACCCACCCCAGAAGG 60.1 0.07
mm4__chrX 72.02 Mbp_chrX_72017129_R GTCTCGTGGGCTCGGCAATAGGCCCTGCTACCCAG 59.8 0.07
mm4__chr8 47.48 Mbp_chr8_47483006_F TCGTCGGCAGCGTCAAATTCCCACTTGCTGGCTG 59.0 0.07
mm4__chr8 47.48 Mbp_chr8_47483006_R GTCTCGTGGGCTCGGCAGACCTGTCCCCTGACTCT 60.2 0.07
mm4__chr12 52.81 Mbp_chr12_52809350_F TCGTCGGCAGCGTCAGGGAGCTGGAGTCCTTTCA 60.1 0.07
mm4__chr12 52.81 Mbp_chr12_52809350_R GTCTCGTGGGCTCGGCTCTATGATGCGGTGAGGGC 60.3 0.07
mm4__chr1 110.21 Mbp_chr1_110209706_F TCGTCGGCAGCGTCGCCAGAGCTACGGTTTCCTT 60.0 0.07
mm4__chr1 110.21 Mbp_chr1_110209706_R GTCTCGTGGGCTCGGATGGTCTTGATGCTCTCCGC 60.1 0.07
mm4__chr2 163.35 Mbp_chr2_163348874_F TCGTCGGCAGCGTCCTCCTCCTTTCCCACTTGCA 59.5 0.07
mm4__chr2 163.35 Mbp_chr2_163348874_R GTCTCGTGGGCTCGGACGCGAAACCCAGTTCAAAT 58.6 0.07
mm4__chr8 64.06 Mbp_chr8_64060317_F Primer3: not found at this Tm N.d. 0.07
mm4__chr8 64.06 Mbp_chr8_64060317_R Primer3: not found at this Tm N.d. 0.07
mm4__chr6 127.64 Mbp_chr6_127641830_F Primer3: not found at this Tm N.d. 0.07
mm4__chr6 127.64 Mbp_chr6_127641830_R Primer3: not found at this Tm N.d. 0.07
mm4__chr3 58.55 Mbp_chr3_58552640_F Primer3: not found at this Tm N.d. 0.07
mm4__chr3 58.55 Mbp_chr3_58552640_R Primer3: not found at this Tm N.d. 0.07
mm4__chr19 36.73 Mbp_chr19_36729830_F Primer3: not found at this Tm N.d. 0.07
mm4__chr19 36.73 Mbp_chr19_36729830_R Primer3: not found at this Tm N.d. 0.07
mm4__chr18 74.10 Mbp_chr18_74096096_F Primer3: not found at this Tm N.d. 0.07
mm4__chr18 74.10 Mbp_chr18_74096096_R Primer3: not found at this Tm N.d. 0.07
mm4__chr17 47.39 Mbp_chr17_47388226_F Primer3: not found at this Tm N.d. 0.07
mm4__chr17 47.39 Mbp_chr17_47388226_R Primer3: not found at this Tm N.d. 0.07
mm4__chr14 21.59 Mbp_chr14_21588267_F Primer3: not found at this Tm N.d. 0.07
mm4__chr14 21.59 Mbp_chr14_21588267_R Primer3: not found at this Tm N.d. 0.07
mm4__chr12 13.41 Mbp_chr12_13410267_F Primer3: not found at this Tm N.d. 0.07
mm4__chr12 13.41 Mbp_chr12_13410267_R Primer3: not found at this Tm N.d. 0.07
mm4__chr11 35.51 Mbp_chr11_35508192_F Primer3: not found at this Tm N.d. 0.07
mm4__chr11 35.51 Mbp_chr11_35508192_R Primer3: not found at this Tm N.d. 0.07
mm4__chr13 111.05 Mbp_chr13_111049052_F Primer3: not found at this Tm N.d. 0.07
mm4__chr13 111.05 Mbp_chr13_111049052_R Primer3: not found at this Tm N.d. 0.07
mm4__chr9 98.72 Mbp_chr9_98723275_F Primer3: not found at this Tm N.d. 0.07
mm4__chr9 98.72 Mbp_chr9_98723275_R Primer3: not found at this Tm N.d. 0.07
mm4__chr4 99.88 Mbp_chr4_99876559_F Primer3: not found at this Tm N.d. 0.06
mm4__chr4 99.88 Mbp_chr4_99876559_R Primer3: not found at this Tm N.d. 0.06
mm4__chr7 100.49 Mbp_chr7_100486785_F TCGTCGGCAGCGTCAGGGTTGCTGACACCTCTTG 59.8 0.06
mm4__chr7 100.49 Mbp_chr7_100486785_R GTCTCGTGGGCTCGGCCTTACTGGAGCTGGGGAGA 60.3 0.06
mm3__chr7 65.40 Mbp_chr7_65404304_F Primer3: not found at this Tm N.d. 0.06
mm3__chr7 65.40 Mbp_chr7_65404304_R Primer3: not found at this Tm N.d. 0.06
mm4__chr15 45.19 Mbp_chr15_45186911_F Primer3: not found at this Tm N.d. 0.06
mm4__chr15 45.19 Mbp_chr15_45186911_R Primer3: not found at this Tm N.d. 0.06
mm4__chr14 69.15 Mbp_chr14_69151683_F Primer3: not found at this Tm N.d. 0.06
mm4__chr14 69.15 Mbp_chr14_69151683_R Primer3: not found at this Tm N.d. 0.06
mm4__chr14 58.65 Mbp_chr14_58646817_F TCGTCGGCAGCGTCGGCTAGCGACTCCGAGTACT 60.8 0.06
mm4__chr14 58.65 Mbp_chr14_58646817_R GTCTCGTGGGCTCGGATTGCTCTGCTCGTCCTCAC 60.1 0.06
mm4__chr22 50.44 Mbp_chr22_50438048_F TCGTCGGCAGCGTCCTCGGAACAGTCGCTTTCCT 60.0 0.06
mm4__chr22 50.44 Mbp_chr22_50438048_R GTCTCGTGGGCTCGGAGAGGGGCTTAGTTCGGGAT 60.0 0.06
mm4__chr19 1.99 Mbp_chr19_1991613_F TCGTCGGCAGCGTCATGACCCGGACAAGAAACCC 59.9 0.06
mm4__chr19 1.99 Mbp_chr19_1991613_R GTCTCGTGGGCTCGGGTCCTCCAATGGGCTTTGGG 60.9 0.06
mm4__chr5 156.44 Mbp_chr5_156439523_F Primer3: not found at this Tm N.d. 0.06
mm4__chr5 156.44 Mbp_chr5_156439523_R Primer3: not found at this Tm N.d. 0.06
mm4__chr19 18.60 Mbp_chr19_18598987_F TCGTCGGCAGCGTCAGGCTGAGGGTCTGGACTAG 60.0 0.06
mm4__chr19 18.60 Mbp_chr19_18598987_R GTCTCGTGGGCTCGGGCTGGCATTTCTCAGCACAG 59.8 0.06
mm4__chr15 87.95 Mbp_chr15_87953912_F TCGTCGGCAGCGTCACACATTGTCTGCAGGCTGT 60.1 0.06
mm4__chr15 87.95 Mbp_chr15_87953912_R GTCTCGTGGGCTCGGGCTGGCAATGGAGGTCGATA 59.8 0.06
mm4__chr7 32.36 Mbp_chr7_32361591_F TCGTCGGCAGCGTCCTGGGCCTTTGCTCTCCTTG 60.9 0.06
mm4__chr7 32.36 Mbp_chr7_32361591_R GTCTCGTGGGCTCGGTGCTGCCCAGGTGACTTAAG 59.9 0.06
mm4__chr13 27.78 Mbp_chr13_27777745_F TCGTCGGCAGCGTCTGGTGAGAGAATACCTGGACA 57.8 0.05
mm4__chr13 27.78 Mbp_chr13_27777745_R GTCTCGTGGGCTCGGGGACATTCATCTGACAGGCGA 60.1 0.05
mm4__chr1 15.62 Mbp_chr1_15616534_F TCGTCGGCAGCGTCCTGCACCCACAGCCATCTTA 60.0 0.05
mm4__chr1 15.62 Mbp_chr1_15616534_R GTCTCGTGGGCTCGGTGCCAGATTAGCATCTTTCCA 57.3 0.05
mm4__chr14 60.25 Mbp_chr14_60245647_F TCGTCGGCAGCGTCTCTCTCAGGTCGCAGGATGA 60.0 0.05
mm4__chr14 60.25 Mbp_chr14_60245647_R GTCTCGTGGGCTCGGTGTGTGACATCACTGGGCAG 60.2 0.05
mm4__chr22 48.61 Mbp_chr22_48610583_F TCGTCGGCAGCGTCGACCACCAGGAGGGCTTTG 60.3 0.05
mm4__chr22 48.61 Mbp_chr22_48610583_R GTCTCGTGGGCTCGGCAACGCTGACAGCTCAACAC 60.0 0.05
mm4__chr17 79.79 Mbp_chr17_79791997_F TCGTCGGCAGCGTCACCTCTATCCCAGCCAGGTG 60.6 0.05
mm4__chr17 79.79 Mbp_chr17_79791997_R GTCTCGTGGGCTCGGGGAGAGGATCCGTCCCTCTT 60.1 0.05
mm4__chr17 36.50 Mbp_chr17_36501669_F TCGTCGGCAGCGTCCACACATCCTGAGGGCCAAA 60.2 0.05
mm4__chr17 36.50 Mbp_chr17_36501669_R GTCTCGTGGGCTCGGTTCCCAAACTAAGGGCTGGG 59.5 0.05
mm4__chr11 36.31 Mbp_chr11_36305686_F Primer3: not found at this Tm N.d. 0.05
mm4__chr11 36.31 Mbp_chr11_36305686_R Primer3: not found at this Tm N.d. 0.05
mm3__chr19 22.88 Mbp_chr19_22883632_F Primer3: not found at this Tm N.d. 0.05
mm3__chr19 22.88 Mbp_chr19_22883632_R Primer3: not found at this Tm N.d. 0.05
mm4__chr10 131.14 Mbp_chr10_131135295_F TCGTCGGCAGCGTCTCTACCTCTGGCTTATTCCGC 59.3 0.05
mm4__chr10 131.14 Mbp_chr10_131135295_R GTCTCGTGGGCTCGGCGGTCACTGGGAAGGTGG 59.6 0.05
mm4__chr1 164.85 Mbp_chr1_164852508_F TCGTCGGCAGCGTCTCCCCAGTGTTCTAGCTGGA 59.8 0.05
mm4__chr1 164.85 Mbp_chr1_164852508_R GTCTCGTGGGCTCGGGCATGTGATGGCCCTAGAGT 59.5 0.05
mm4__chr6 461.21 Kbp_chr6_461206_F Primer3: not found at this Tm N.d. 0.05
mm4__chr6 461.21 Kbp_chr6_461206_R Primer3: not found at this Tm N.d. 0.05
mm4__chr11 124.85 Mbp_chr11_124845424_F TCGTCGGCAGCGTCCACCTTCCCACCAGTCTGAC 59.9 0.05
mm4__chr11 124.85 Mbp_chr11_124845424_R GTCTCGTGGGCTCGGTCTAGACACAAGCTCCTTTGGA 58.7 0.05
mm4__chr10 11.38 Mbp_chr10_11381496_F TCGTCGGCAGCGTCGCACAGAGAAAGCAGTGTGC 60.0 0.04
mm4__chr10 11.38 Mbp_chr10_11381496_R GTCTCGTGGGCTCGGCCGTCTGTCTCTCTGCCTTC 59.8 0.04
mm4__chr6 6.65 Mbp_chr6_6654122_F Primer3: not found at this Tm N.d. 0.04
mm4__chr6 6.65 Mbp_chr6_6654122_R Primer3: not found at this Tm N.d. 0.04
mm4__chr1 155.29 Mbp_chr1_155289227_F Primer3: not found at this Tm N.d. 0.04
mm4__chr1 155.29 Mbp_chr1_155289227_R Primer3: not found at this Tm N.d. 0.04
mm4__chr20 50.70 Mbp_chr20_50703600_F Primer3: not found at this Tm N.d. 0.04
mm4__chr20 50.70 Mbp_chr20_50703600_R Primer3: not found at this Tm N.d. 0.04
mm4__chr4 1.30 Mbp_chr4_1299342_F Primer3: not found at this Tm N.d. 0.04
mm4__chr4 1.30 Mbp_chr4_1299342_R Primer3: not found at this Tm N.d. 0.04
mm3__chr14 84.99 Mbp_chr14_84991950_F TCGTCGGCAGCGTCGGACGAGTTGCTGAATTGTTCA 59.4 0.04
mm3__chr14 84.99 Mbp_chr14_84991950_R GTCTCGTGGGCTCGGGGTCATTAAACGTATCTTTCCGTCA 59.4 0.04
mm4__chr9 22.82 Mbp_chr9_22819991_F TCGTCGGCAGCGTCAGCTGTTGCAATTACAAAATGATCA 58.5 0.04
mm4__chr9 22.82 Mbp_chr9_22819991_R GTCTCGTGGGCTCGGCCTGAAAGATTAACCCTCTATCATG 57.0 0.04
mm4__chr7 151.43 Mbp_chr7_151431361_F TCGTCGGCAGCGTCTGGGGACGTGGACCTCTTAT 59.9 0.04
mm4__chr7 151.43 Mbp_chr7_151431361_R GTCTCGTGGGCTCGGGTTGGGGTGGGCTTGAATCT 60.2 0.04
mm4__chr14 21.03 Mbp_chr14_21032659_F TCGTCGGCAGCGTCTTTCAACACCCAGCCCAGAA 59.7 0.04
mm4__chr14 21.03 Mbp_chr14_21032659_R GTCTCGTGGGCTCGGTGCTCTTGGAATCCTGGCAG 60.0 0.04
mm3__chr8 144.20 Mbp_chr8_144202444_F Primer3: not found at this Tm N.d. 0.04
mm3__chr8 144.20 Mbp_chr8_144202444_R Primer3: not found at this Tm N.d. 0.04
mm4__chr1 156.69 Mbp_chr1_156691446_F TCGTCGGCAGCGTCGGAACGTAGGCTGTGGGAAA 59.9 0.03
mm4__chr1 156.69 Mbp_chr1_156691446_R GTCTCGTGGGCTCGGAACCAGCTTAGTCCCAACGG 59.9 0.03
mm4__chr5 78.94 Mbp_chr5_78938040_F Primer3: not found at this Tm N.d. 0.03
mm4__chr5 78.94 Mbp_chr5_78938040_R Primer3: not found at this Tm N.d. 0.03
mm4__chr1 75.91 Mbp_chr1_75909148_F Primer3: not found at this Tm N.d. 0.03
mm4__chr1 75.91 Mbp_chr1_75909148_R Primer3: not found at this Tm N.d. 0.03
mm4__chr22 19.68 Mbp_chr22_19680631_F Primer3: not found at this Tm N.d. 0.03
mm4__chr22 19.68 Mbp_chr22_19680631_R Primer3: not found at this Tm N.d. 0.03
mm4__chr22 45.67 Mbp_chr22_45674072_F Primer3: not found at this Tm N.d. 0.03
mm4__chr22 45.67 Mbp_chr22_45674072_R Primer3: not found at this Tm N.d. 0.03
mm4__chr6 134.11 Mbp_chr6_134112764_F Primer3: not found at this Tm N.d. 0.03
mm4__chr6 134.11 Mbp_chr6_134112764_R Primer3: not found at this Tm N.d. 0.03
mm4__chr4 189.06 Mbp_chr4_189062027_F Primer3: not found at this Tm N.d. 0.03
mm4__chr4 189.06 Mbp_chr4_189062027_R Primer3: not found at this Tm N.d. 0.03
mm4__chr10 129.46 Mbp_chr10_129461459_F TCGTCGGCAGCGTCCCTGCCTTTCCTTCTCTGGG 60.0 0.03
mm4__chr10 129.46 Mbp_chr10_129461459_R GTCTCGTGGGCTCGGTTCTTGGTGAGGGCAGTGTC 59.8 0.03
mm4__chr19 57.04 Mbp_chr19_57035735_F TCGTCGGCAGCGTCGGGATTTCCACAGGGACAGG 60.0 0.03
mm4__chr19 57.04 Mbp_chr19_57035735_R GTCTCGTGGGCTCGGTAAACCCCAGGGATCTGTGC 59.3 0.03
mm3__chr19 14.20 Mbp_chr19_14197726_F Primer3: not found at this Tm N.d. 0.03
mm3__chr19 14.20 Mbp_chr19_14197726_R Primer3: not found at this Tm N.d. 0.03
mm4__chr19 5.10 Mbp_chr19_5098748_F Primer3: not found at this Tm N.d. 0.03
mm4__chr19 5.10 Mbp_chr19_5098748_R Primer3: not found at this Tm N.d. 0.03
mm4__chr4 7.39 Mbp_chr4_7394617_F TCGTCGGCAGCGTCTGCTGGGGTTTAGAATGAGGG 59.7 0.03
mm4__chr4 7.39 Mbp_chr4_7394617_R GTCTCGTGGGCTCGGCCCAATCACCCAGCAAGACT 59.9 0.03
mm4__chr20 62.51 Mbp_chr20_62514784_F TCGTCGGCAGCGTCACTTGCTTTTGTCCGACCCT 59.8 0.03
mm4__chr20 62.51 Mbp_chr20_62514784_R GTCTCGTGGGCTCGGCACCTGTGTGGGTCCCAAC 60.5 0.03
mm4__chr20 21.82 Mbp_chr20_21821884_F Primer3: not found at this Tm N.d. 0.03
mm4__chr20 21.82 Mbp_chr20_21821884_R Primer3: not found at this Tm N.d. 0.03
mm4__chr11 60.93 Mbp_chr11_60930503_F TCGTCGGCAGCGTCCCTTCAATCCAGCCCACAGT 59.9 0.02
mm4__chr11 60.93 Mbp_chr11_60930503_R GTCTCGTGGGCTCGGCAGCTTGGCAGTCCAGAGTG 60.9 0.02
mm4__chr18 12.25 Mbp_chr18_12245840_F TCGTCGGCAGCGTCCTGGCTCTTTCTGGTCAGGG 60.0 0.02
mm4__chr18 12.25 Mbp_chr18_12245840_R GTCTCGTGGGCTCGGTTCCTCATGCCCGGGTTTTG 60.8 0.02
mm3__chr12 56.61 Mbp_chr12_56611570_F TCGTCGGCAGCGTCTCAGAGGAAAAGAGGCATTCCC 60.0 0.02
mm3__chr12 56.61 Mbp_chr12_56611570_R GTCTCGTGGGCTCGGGGAGACGTATTGCTACCCCAG 59.9 0.02
mm4__chr16 20.62 Mbp_chr16_20623403_F TCGTCGGCAGCGTCTCAACACTCTCAATGCCCCA 59.2 0.02
mm4__chr16 20.62 Mbp_chr16_20623403_R GTCTCGTGGGCTCGGACCTAAGGCAAATCCCTGGC 60.0 0.02
mm4__chr11 75.72 Mbp_chr11_75717017_F Primer3: not found at this Tm N.d. 0.02
mm4__chr11 75.72 Mbp_chr11_75717017_R Primer3: not found at this Tm N.d. 0.02
mm4__chr3 73.74 Mbp_chr3_73737265_F TCGTCGGCAGCGTCTCCACGTGTGTTGGCAAAAC 59.8 0.02
mm4__chr3 73.74 Mbp_chr3_73737265_R GTCTCGTGGGCTCGGGGGACCCATGAATGCCAGAA 60.0 0.02
mm4__chr14 105.17 Mbp_chr14_105170416_F Primer3: not found at this Tm N.d. 0.02
mm4__chr14 105.17 Mbp_chr14_105170416_R Primer3: not found at this Tm N.d. 0.02
mm3__chr10 127.13 Mbp_chr10_127134952_F TCGTCGGCAGCGTCAGGCTAGTGTCAGCAGGAGA 59.9 0.02
mm3__chr10 127.13 Mbp_chr10_127134952_R GTCTCGTGGGCTCGGTCCATTGAGCACATCCCCAG 59.7 0.02
mm4__chr20 50.24 Mbp_chr20_50243253_F TCGTCGGCAGCGTCGGCACAAAGGCCCATTGTTC 60.3 0.02
mm4__chr20 50.24 Mbp_chr20_50243253_R GTCTCGTGGGCTCGGGTGAATCGCTCCTCAGTCCC 60.1 0.02
mm4__chr3 194.75 Mbp_chr3_194754388_F Primer3: not found at this Tm N.d. 0.02
mm4__chr3 194.75 Mbp_chr3_194754388_R Primer3: not found at this Tm N.d. 0.02
mm4__chr12 109.78 Mbp_chr12_109776531_F TCGTCGGCAGCGTCAACACAAGACTCCCAGCCTG 59.8 0.02
mm4__chr12 109.78 Mbp_chr12_109776531_R GTCTCGTGGGCTCGGGTGATGGAGCCAGAGGTCAG 59.8 0.02
mm4__chr12 111.78 Mbp_chr12_111783914_F Primer3: not found at this Tm N.d. 0.02
mm4__chr12 111.78 Mbp_chr12_111783914_R Primer3: not found at this Tm N.d. 0.02
mm4__chr17 45.45 Mbp_chr17_45452137_F TCGTCGGCAGCGTCACCTAAGGGTCCCATGGTGA 59.8 0.02
mm4__chr17 45.45 Mbp_chr17_45452137_R GTCTCGTGGGCTCGGCCATTGTTAGCCCCTCCCAC 60.3 0.02
mm4__chr5 6.18 Mbp_chr5_6181146_F TCGTCGGCAGCGTCCTCCACACCTCACCACCATC 60.0 0.02
mm4__chr5 6.18 Mbp_chr5_6181146_R GTCTCGTGGGCTCGGAGCACCACAGGAGAGAAGGA 60.1 0.02
mm4__chr4 4.62 Mbp_chr4_4620889_F Primer3: not found at this Tm N.d. 0.01
mm4__chr4 4.62 Mbp_chr4_4620889_R Primer3: not found at this Tm N.d. 0.01
mm3__chr1 33.97 Mbp_chr1_33965180_F TCGTCGGCAGCGTCTCATCAAGCTTCCCCTTGGT 58.9 0.01
mm3__chr1 33.97 Mbp_chr1_33965180_R GTCTCGTGGGCTCGGGTGGAAAGTGGGAAGCAGGA 59.8 0.01
mm4__chr5 2.80 Mbp_chr5_2801820_F TCGTCGGCAGCGTCGAATGATGAGGGCGGAGAGG 59.9 0.01
mm4__chr5 2.80 Mbp_chr5_2801820_R GTCTCGTGGGCTCGGGCACTTTGAAGGAATACAGCTGT 59.4 0.01
mm4__chr13 43.97 Mbp_chr13_43971040_F TCGTCGGCAGCGTCGAGACCTGTTTGCACCAAGC 59.6 0.01
mm4__chr13 43.97 Mbp_chr13_43971040_R GTCTCGTGGGCTCGGCTCGGTGTCCTGAGCCTTC 59.7 0.01
mm4__chr11 46.38 Mbp_chr11_46379512_F TCGTCGGCAGCGTCGAGACCTGTTTGCACCAAGC 59.6 0.01
mm4__chr11 46.38 Mbp_chr11_46379512_R GTCTCGTGGGCTCGGTCTGTCTTCATGAGCGAGGC 59.8 0.01
mm4__chrX 109.83 Mbp_chrX_109825131_F TCGTCGGCAGCGTCGGACCTCATATGCAGCAGCT 59.8 0.01
mm4__chrX 109.83 Mbp_chrX_109825131_R GTCTCGTGGGCTCGGAGACTTCTCCATGGTGGACT 57.6 0.01
mm4__chr10 129.19 Mbp_chr10_129192607_F TCGTCGGCAGCGTCGGCTGGCCATTTCCTTCTTG 59.4 0.01
mm4__chr10 129.19 Mbp_chr10_129192607_R GTCTCGTGGGCTCGGACTGTTGCAATGTCCTAGCGA 59.9 0.01
mm4__chr2 239.48 Mbp_chr2_239482524_F TCGTCGGCAGCGTCTGGGAAGAATTTCACCGGGA 58.9 0.01
mm4__chr2 239.48 Mbp_chr2_239482524_R GTCTCGTGGGCTCGGTCGATATGCAAAACATCACGACA 59.0 0.01
mm4__chr1 20.39 Mbp_chr1_20391818_F Primer3: not found at this Tm N.d. 0.01
mm4__chr1 20.39 Mbp_chr1_20391818_R Primer3: not found at this Tm N.d. 0.01
mm4__chrX 109.82 Mbp_chrX_109817510_F TCGTCGGCAGCGTCAGACTTCTCCAAGGTGGACT 57.5 0.00
mm4__chrX 109.82 Mbp_chrX_109817510_R GTCTCGTGGGCTCGGTCACCCAGAAAGGCCTTGTC 59.8 0.00
mm4__chr10 120.59 Mbp_chr10_120591327_F TCGTCGGCAGCGTCCTGGTCCTTCCAAACTGGCT 59.8 0.00
mm4__chr10 120.59 Mbp_chr10_120591327_R GTCTCGTGGGCTCGGTGCTTGCCAATGTAACTGCT 58.3 0.00
mm3__chr11 120.47 Mbp_chr11_120470451_F Primer3: not found at this Tm N.d. 0.00
mm3__chr11 120.47 Mbp_chr11_120470451_R Primer3: not found at this Tm N.d. 0.00
mm2__chr2 96.13 Mbp_chr2_96131842_F TCGTCGGCAGCGTCTGAAAGGTCTCGTGCTGGAC 59.9 0.00
mm2__chr2 96.13 Mbp_chr2_96131842_R GTCTCGTGGGCTCGGTGGGCTTGATGGTCTGGTTC 59.9 0.00
mm4__chr4 26.61 Mbp_chr4_26606473_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 26.61 Mbp_chr4_26606473_R Primer3: not found at this Tm N.d. 0.00
mm4__chrX 145.72 Mbp_chrX_145715928_F Primer3: not found at this Tm N.d. 0.00
mm4__chrX 145.72 Mbp_chrX_145715928_R Primer3: not found at this Tm N.d. 0.00
mm4__chr8 141.03 Mbp_chr8_141031320_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 141.03 Mbp_chr8_141031320_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 34.55 Mbp_chr6_34554131_F TCGTCGGCAGCGTCGGCGGGACCTCACCTGTT 61.6 0.00
mm4__chr6 34.55 Mbp_chr6_34554131_R GTCTCGTGGGCTCGGTGACTTGTTGCAGCCTGAGT 59.8 0.00
mm4__chr5 1.10 Mbp_chr5_1102563_F TCGTCGGCAGCGTCCTTAGACTCAGCCTCAGCCG 59.8 0.00
mm4__chr5 1.10 Mbp_chr5_1102563_R GTCTCGTGGGCTCGGCTGGGTAGCTGGACGGAC 59.1 0.00
mm4__chr3 194.97 Mbp_chr3_194971078_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 194.97 Mbp_chr3_194971078_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 182.02 Mbp_chr2_182021097_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 182.02 Mbp_chr2_182021097_R Primer3: not found at this Tm N.d. 0.00
mm4__chr18 51.53 Mbp_chr18_51528613_F Primer3: not found at this Tm N.d. 0.00
mm4__chr18 51.53 Mbp_chr18_51528613_R Primer3: not found at this Tm N.d. 0.00
mm4__chr18 38.78 Mbp_chr18_38781269_F Primer3: not found at this Tm N.d. 0.00
mm4__chr18 38.78 Mbp_chr18_38781269_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 31.91 Mbp_chr11_31906570_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 31.91 Mbp_chr11_31906570_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 57.88 Mbp_chr1_57883441_F TCGTCGGCAGCGTCTGCAAAGCCAGACTGCATTG 59.6 0.00
mm4__chr1 57.88 Mbp_chr1_57883441_R GTCTCGTGGGCTCGGAATTTTGCACAGCACCAGGC 60.2 0.00
mm4__chr1 208.02 Mbp_chr1_208016929_F TCGTCGGCAGCGTCAGGCAGAGGAGAAGTCAGGT 59.8 0.00
mm4__chr1 208.02 Mbp_chr1_208016929_R GTCTCGTGGGCTCGGCCCCACAGAAGAGCCTACAG 59.4 0.00
mm3__chr16 5.91 Mbp_chr16_5905903_F TCGTCGGCAGCGTCCACTCCCCAAATGCCTCCTC 60.3 0.00
mm3__chr16 5.91 Mbp_chr16_5905903_R GTCTCGTGGGCTCGGCCTAAGGTAGGGCTTTGGGC 60.1 0.00
mm3__chr12 76.72 Mbp_chr12_76715361_F Primer3: not found at this Tm N.d. 0.00
mm3__chr12 76.72 Mbp_chr12_76715361_R Primer3: not found at this Tm N.d. 0.00
mm3__chr1 112.00 Mbp_chr1_112004368_F TCGTCGGCAGCGTCTCTTTCTGCCCATAGCCTGG 59.4 0.00
mm3__chr1 112.00 Mbp_chr1_112004368_R GTCTCGTGGGCTCGGTGTTTGCCTCTGAGGAAGACC 59.9 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529549 CTCCCCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCT
TGCACATGCCGGAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATCCA
mm2__chr15 43.99 Mbp_chr15_43990074 GAGTCCTTCTGACCCATGCCCACCGTCACGCCCTGGTGTCTGGGGCGCCCCACAATGAAGGGGAAGATGGCCCGGGGTGG
GCATCGTCACCTGCAAAGCTGGCCTTGCACATGCCAGAGCCATTGTCGACGACA
mm1__chr6 88.28 Mbp_chr6_88276899 GAGTCCTTCTGACCCATGCCCACCATCACTCCCTGGTGCCTAGGGTGCCCCACAATGGAGGGGAAGACGGCCTGGGGAGC
CTTGCGCATGCTGGAGCAGTTGTCGACGACGACGAGCGCGGTGATAGCATCATCCA
mm0__chr19 9.52 Mbp_chr19_9518234 AGTTAACAATGGCTCCGGCATGTGCAAGGTCAACTTCGTGGGCGACAATGCCCCCCGGGCCATCTTCCCCTCCATCGTGG
GGCATCTGAAGCACCAGGGCGTTATGGTGGGCATCGGTCAGAAGGACTCC
mm0__chr11 1.80 Mbp_chr11_1803987 CGCCACCCACATAGAAGTCCTTCTGACCCATGCCCACCGTCATGCCCTGGTGTCTGGGGCACCCCACGATGGAGGGGAAG
ACGGCCCAGGGGGCATCGTCGTCGGTGAAGCTGGCCTTGCACATGCCAGAGGTGTT
mm1__chr22 41.08 Mbp_chr22_41075131 GGAGTCCTTCTGACCCATGCCCACCATCATGACCTGGTGCCTGGTGCACCCCACGATGGAAGGGAAGATGGCCCAGGGGA
CATCGTCACCCGCGAAGCCGGCCTTGCACATGCCAGAGCTGTTGTCAACA
mm4__chr5 98.34 Mbp_chr5_98338827 GCTCCTCATCAACAAGGGCTCTGGCATGTGCAAGGCCGGCTTCACAGGTGACAATGCCCCCGGGCCATCTTCCCCTCTGT
CATGGGTGCCAGGGAGTGATGGTGGGCATGGGTCAAAA
mm2__chr18 62.44 Mbp_chr18_62442093 CCTCGTCATCGACAATGGCTCTGGCATGTGCAGGGCTGGCTTCGCAGGCGACAGTGCTCTCCAGGCCGTCTTCCTCTCCA
TCATGGGGCACCCCAGGCACCAGGGTGTGATGGTGGGCATGGGTCAGAAGGACTC
mm2__chr1 223.86 Mbp_chr1_223864743 TGACCCATGCTCACCATCACGCCCTGGTGCCTGGGGCGCCCCACCATGGAAGGGAAGATGGCCCAGGGGTCATCATCCCC
TGTGAAGCCAGCCTTGCACATGCCGGAGCTGTTGTCAATGACGAGCG
mm4__chr17 16.96 Mbp_chr17_16963354 TAAGGAGTGCTGTGCTGGACAAGGCCTACACCTGGATCGAGACAACAAAAATTTTAGGGGCCAATTCCTGTTGGCGTGAG
CCAACTCCCTCTTTTCCTCCTTGGTGGGGAGGTGTCTGTGCAGGAGTGAA
mm4__chr22 29.49 Mbp_chr22_29490252 AGCAAGAAAGAGGAGGCACCCAAGAAGGAGGCTCCAAAGCCCAAGGTGGAGGAGAAGAAGGAACCTGCTGTCGAAAAGCC
CAAAGAATCCAAAGTTGAAGCCAAGAAGGAAGAGGCTGA
mm2__chr5 131.66 Mbp_chr5_131660008 ATGCCCATCATCACACCCTGGTGCCTCGGGTGCCCCATTATGGAGGGGAAGACGGCCCGGGGGCATCGTTGCCCGTGAAG
CAGACCTTCCACA
mm4__chr18 38.54 Mbp_chr18_38541461 GGATAAGACCCATGTTCCTCCTCCATCTTCCTCTCCACCATGGAGGCTGGACAGGAAGGATTAGTCCAACCTGAGCCTCA
CAAGACCCACGCATCT
mm4__chr7 157.85 Mbp_chr7_157849859 GGGGAGGCCAAACGTGTTATTTTAGCTAAACTTAGGGTTCGATCACCATGGAGGAGGGGAAGACGGCACAGACCCTTCAC
CGCTGGCCCTGATGGCAGCTGGGGCCCGTGTGTGCCGTGTCCTGGGGACGACATGAGAT
mm4__chr5 1.07 Mbp_chr5_1068507 GCCCAGATTTCTCTAATTTCACACTAACATAGCCACGATGGAGGAGAATACGGCTCCCCAGGGCAGTGCAGCCCCAAAGG
CAGCCAAGGGAAGG
mm3__chr12 26.33 Mbp_chr12_26334147 CCAAGATCACAAAGATACACCTGACCCACGTTGAAGGGGAAGAGGGAGTGAGTTATGGTATAGAGAAAGGAGGATACAAT
TGTCCAAAGTGAAGATAATTATTACCAAAAAAACCAGAAAGCACAGTCATTAGAGCAGGG
mm4__chrX 56.62 Mbp_chrX_56618391 GCAGGGAAGGAAGCCAAGTATGTCATAGTAGAGGAGGAGGGTCTACATACTTCCTTGTATTAAAAAGGGAAACTCATGAA
GCACCAAAGCACACAACGAAGGAGAGGAAGAAGGGAGGAAGGCACTTCACAAGC
mm4__chr3 69.10 Mbp_chr3_69097735 GCAGAGAGGCCAAGTAGACCCAGGTAACACAGAACCAAGAGGGAGGGGAAGAGGGCAGAGAGAATAGCACATGTGCTGGG
TATTGGGGAAGGTTGGG
mm4__chr2 174.16 Mbp_chr2_174157340 AGCCTAACAGGGGAGACAGATCAGCATATAATAAAAACAAGAAAGTAAACCCAAGATGAAGGGGAACAGGGAAGGAGTCA
GTATTCACACAAGTTGATGGAAAAAGACAGCCTTCAAGGTTTCA
mm4__chr8 84.95 Mbp_chr8_84948673 CTCTGGTATGTGCAAGGCCAGAATCCTTGTCAAAGATGCCCCCAGGCCTTCTTCCTTTCCATAGTGGGATGCCCATGGGA
CCAGAGTTTGATGATGGGCATAGGGCAGAAGGACTG
mm4__chr1 181.48 Mbp_chr1_181481588 GTTTCCTTCCTCAGCCCCTCCTTTCCTTCTTCCCTTCCATTGTTCGGGTTGGAAGGATTTGGAGAGGCCTATGTGTTGAG
CCAAGGCAGGAAAACACAG
mm3__chr6 101.43 Mbp_chr6_101431429 TGATGCTGTCCTGTGTGCTTACTAGAAAGACAAGTCTTTACTCTGACAGAGTTTATATTCTGGTGCTGGGGGCGCCCAAC
AATGGAGGGGAACATGGCTTGGGGGCGTCATCCCCAGCAAAGCCG
mm4__chr19 49.37 Mbp_chr19_49374718 CCTTGTTCTTCCTCCCCAGGTACCCAGGATGGAGGAGAAGGAGGCCCTGGTACCCATCCAGAAGGCCACGGACAGCTTCC
ACACAGAACTCCATCCCC
mm4__chr10 79.18 Mbp_chr10_79179097 AGCTGTGACTGCTGACTCACACATGAACATTTCTTAAACATCTGCTATCTCTGTGCTGCAGGCCAGGAACCCAGGATGGA
GAAGAAGAGGGATGCTAGCACATAAAACACTTACTGTATGTGCCAGCCCTCCAAG
mm4__chr10 58.84 Mbp_chr10_58837539 TAATTGGGAGGTGAGGGGCAGAGAGCAGGACCACCCTCCCCACGATGGAAGGAATGGAGGGGACTCCACAGCAAGTGTGA
AGGTGAGCTGTGACCAGAGCTGAAC
mm4__chr6 16.08 Mbp_chr6_16078009 TACAGCCCAATGAAGCCCAAATACACACAATAATATTACCAACTTCTCCTCCCTTGTGGGGGACACAAACGAAGTCCTGG
T
mm4__chr5 168.05 Mbp_chr5_168047124 AGCAACTGTGTCCAGAGTGTAGAAAAGGATGGATGTGGTAGAGCAGCACTCTTTACAGTGATTCGAAAGGGCCATTTTCC
CCTCCATGGTGGCACCGTTTGGAAGATCTGGGCT
mm4__chr5 15.45 Mbp_chr5_15446234 TCATTCTTCAGGTGGCAGCGCATTGCTTTCCTGAGCCTATTGCTCCAAGAGAATCAAAATGTGGGCTCTTGTCCCCCAAG
TGGAGGGGAAGATGGGTGCTTTGTGTCCGCTTTGGTCTCACTGA
mm4__chr10 116.08 Mbp_chr10_116082380 TTCCTTCTTGGCCTGGTGTCCAAAGAACATTCTCCCTCTTCCCCTCCTCCCTGGGACTTTCCACCAAGACCACATCTTAG
AACTGGCAGGATATCATTCAGCTCAAACCGCCC
mm3__chr19 30.69 Mbp_chr19_30685762 TAAAGAAATGGCCCCGTGCAGAGGCAACCAGGGGGATGGGGGCACCGTCCCAGCCGCTGCCGCCGTCCTCCCCTCCATCA
TGGGCTGGGACGCTGTCAGCTCCACCACCTGCCCCACGGTCCTGCCACGTTGCTGAGATC
mm4__chr20 42.78 Mbp_chr20_42782235 TGTTCAATGCCTGGGAGGTCTGTAGGCATTCCTCCTTCTCCATCTTCCCTTCTAACGTGGGCCCCTGTTAGTCATCTTTT
CACTGCATACACTCTCCCTTCAACAGCTGC
mm3__chr10 71.74 Mbp_chr10_71742343 CAGAAAGGGTGCCTCTCTGGCCCTCTTCCCCTACATTGTGTGGTGTGGCATGCTGTCACAACACGTCAGGGT
mm3__chr3 149.42 Mbp_chr3_149421159 TCTTTTCTCAACAGTGATCCTACCTTATATAATTTTGTATGTGTATTCTTTCACTAAAGTATATCTAAAGCATTTTCCCC
ACGATGGGGAGGAAGTTGGATCCACCAGTTGGTGGGGAGGAGAGGAGGTCACAA
mm4__chr11 2.41 Mbp_chr11_2412145 ACCTGAGTGGCTTCATCTGGAAGCCTGCCCACAAGCCGCCCTGAGGCCACACGGCCTCTGGGCCCCACAGACCTGCAGGT
GACACCCACGATGAACAGGAAGATGGCCACCATGTCACACTTGTTCCAGTTGTCCCC
mm3__chr6 46.21 Mbp_chr6_46205060 CGGCATGTGAAAAGCAGCTTTGCTGGGACAATGCCCTCCGAGCCATATTCCCCTCCATCATCGGGCACCCCCGGCACCAG
GGCGTGATGGTGGGCATGGGCCAGAAGGACTCCTACGTG
mm4__chr1 68.12 Mbp_chr1_68123474 TGTCTGGCTCACCACTCTTGGTGGTCATCTGCTTGATGTTTTACTGCTCTGCCCAGTTGTCCTTCTTCCTCTCAATGGTG
GTGGCTGCCCAATCTGCTGGACCCCTCAG
mm4__chr2 100.97 Mbp_chr2_100969042 TAGATGCTGCCTTTGTGGGGTCACTGTTCCCAAGGATGAAGGGGAAAATGGTCTTCTCCCCGACTACGGAAATTTTAAGA
ACGTAAATTAGTTTTAAGCTGGCCTACAGAGGAGCCTTGGAGAAGG
mm1__chr3 175.98 Mbp_chr3_175978471 GAGTCCTTCTGACCCATGCCCACCATCACACCTTGGTGCCTGGGGCGCCCCACGATGGATGGGAAGACGGCCCCGGAGGC
ATCGTCGCCTGCGAAGCTGGTCTTGCACATACAGGAGCCTTTGC
mm4__chr12 123.41 Mbp_chr12_123407476 ACCAGAGCTGAGCACAAGTGTGACTCTCTTCAGGTTGAAAAGCCTCTTTATCCATTTAATCCTCTCTGGCCCTCCTTCCC
CTCCAGCGTGGATGCAACTAGAGAGACAAATCGCCTAGGAAGACTGATCA
mm4__chr21 36.49 Mbp_chr21_36493842 GAACACATGTGGCTGGAGGAAGGTGGGATGGATGAGCACACGCTGGGATTCCTCACAGCCTTCATCTCCTCCATGGTGTG
GAGGGGACCCAGGATCCTCATGTGTTCCCAAAGCAGGTATAGAGTGGCTGGGCA
mm4__chrY 18.15 Mbp_chrY_18147968 ACAATGGCTCTTGCATGTGCAAAGCTGGCTTTGCAGGGGACGATGCTCCCCAAGCCATGTTTCCCTCCACCGTTGGGTTC
CCCGGACACCAGGGCGTGATGGTGGACATGGGCCAGAAGGACTC
mm4__chrY 17.76 Mbp_chrY_17758017 GAGTCCTTCTGGCCCATGTCCACCATCACGCCCTGGTGTCCGGGGAACCCAACGGTGGAGGGAAACATGGCTTGGGGAGC
ATCGTCCCCTGCAAAGCCAGCTTTGCACATGCAAGAGCCATTGT
mm4__chr10 112.22 Mbp_chr10_112218299 GATGCCATACAGAGCAGGGGCACACGGAAATGGATTAAGGATCATGGGAGTCTCAAGATGCAGGGGAAGATGGCTACAAA
GGAGAGAAAACAGAGCGACTATGGAGAGATGGAGAGGACTTCACTGAAGGCTCGCCAAG
mm4__chr8 10.70 Mbp_chr8_10698402 CAGCTTTGGAACTGCTGCAGATTATCCAGAGCCGGCATGCCCCTAGGATGGGGGAGAAGATGGAGGAGGCAGGGACATGG
GGACAGCTTGGGGAGGGTCATGTGAC
mm3__chr12 89.12 Mbp_chr12_89123196 ACCAGATGAATATGTTAAATGCACCTTACAATATTTGAGATCTTCAAAAGAAAAAGATGGAGCTCACGTTGGAGGGGAAG
AAGGAAGAAAGAGGAGGAATGGAAAAGGGAGTAGAATGCTTTTCTTGGGTGTACATGC
mm3__chr5 14.27 Mbp_chr5_14268293 TGGCCTGGAATATGACAGCCCTCACTTCCATCTTCCACTCCATTGTGGGTATCAGTAAATAGGTGTGATTCAGAAAGTTA
GCAGTAATTGTCAAAAATGCTTTGGAATGATGACTCAGGTCACACAGCTCT
mm4__chr12 114.25 Mbp_chr12_114245200 GTTACCTCCCTGGCATCTGCATCCCCTGAACCCTCTTTCCCACCATCTTTGGGATCAGTCCTCCTGTTCTTGTTCAATCT
CTGAAAATCTTGCGGTTTCTGGATAAATGCCAGTGACCCC
mm4__chr19 10.40 Mbp_chr19_10404842 GCTTCATTCAAACCCGCGAGTGGGCGGGCAGGGGCGGGGGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNG
GCAGTCCAGCACATCAGTGGCC
mm4__chr1 202.87 Mbp_chr1_202873376 TGCTGGGTATAGTTCCCCGAGCCATGTTCCCCTCCATCACTGGGTATCCCTGGCACCAGGGCATGATGGTGGGCATAGGC
CAGAAGGACTCC
mm4__chr12 121.44 Mbp_chr12_121442965 GTGCAGCTCTAACCGCTCAGGCCTGGGGCACAGGAGGGAGGGGAAGATGGTACCTTTTTCCTGAAAAGCTTATCTTCTTT
GCCAGGTTTGAGCTGTCA
mm1__chr22 15.72 Mbp_chr22_15721571 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm1__chr2 131.26 Mbp_chr2_131263640 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGCGCCCCAGGCAGCAGGGCATGATGGGGGGCATGCATCAGAAAGAG
mm1__chr2 130.66 Mbp_chr2_130656834 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCAGCAGGGCATGATGGGGGGCATGCATCAGAAAGAG
mm1__chr2 130.46 Mbp_chr2_130463835 CTCTTTCTGATGCATGCCCCCCATCATGCCCTGCTGCCTGGGGCGCCCCACGATGGAAGGGAAGACAGCCCGGGGGGCAT
CGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm1__chr2 130.08 Mbp_chr2_130075263 CTCTTTCTGATGCATGCCCCCCATCATGCCCTGCTGCCTGGGGCGCCCCACGATGGAAGGGAAGACAGCCCGGGGGGCAT
CGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm1__chr14 19.40 Mbp_chr14_19402800 ATCATGTTCTGGTGCCTGGGGCACCCCACGATGGAAGGGAAGACAGCCCGGGGGGCATCGTCACCTGCAAAGCCGGCCTT
GCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm1__chr14 19.00 Mbp_chr14_18998952 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm2__chr2 73.90 Mbp_chr2_73901399 AGGCTTCGCAGGAGATGATGCCCCCCGGGCTGTCTTCCCCTCCATTGTGGGCCGCCCTCGCCACCAGGTGCGTGCTCATC
TGGATA
mm4__chr5 179.78 Mbp_chr5_179776594 CACCTTCCCTACCTTTCCCATGTACTCAGTTTAGCTCTCAAAGAAGGGGTGAATCATAAAGCCAGTGAAAATTTCACCCT
CTGAGGGAGTTCCCCAATCTGAAGGGGAAGAGGGTGACCTCAGCGGCTTTTCTC
mm4__chr11 123.75 Mbp_chr11_123754200 GTGGTGAAGGGGATTGCAGATGGTGAGGTAGCGGTCAAAAGACATGATAGTGAGGATCAAGAACTCGGTGGTGCCCACGA
AGAAGTGGAAGAAGGCCTGCAGCAGGCAGCAGGACAT
mm3__chr16 50.06 Mbp_chr16_50059123 CGCCCACATAGGAGTCCTTCTGGCCCATCCATGCCCACCATCATGAGCTGGTGCCCGGGGCACCCGACAATGGAGGGGAA
CATGGTTCAGGGGGCATCGTCCCTAGCAAAGCCA
mm2__chr2 131.63 Mbp_chr2_131626223 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCAGGCTGTCTTCCCTTC
TATCGTGGGGCGCCCCAGGCACCAGGGCATGATGGAGGGCATGCATCAGAAGGAGT
mm4__chr22 17.02 Mbp_chr22_17015514 ATGCTCAAAAGGAAGGGCCAGGTTTCCCTGACACAGTGGGCTCCGTGGTCTCCTCCCCTCCTTTTCACCTCAGGAGGGAG
GGAAAGAGGGGAAGAGAAGGTCCTGCAGAGGA
mm4__chr22 11.02 Mbp_chr22_11017397 TCCTCTGCAGGACCTTCTCTTCCCCTCTTTCCCTCCCTCCTGAGGTGAAAAGGAGGGGAGGAGACCACGGAGCCCTCTGT
GTCAGGGAAACCTGGCCCTTCCTTTTGAGCGTTCAGGGGACTC
mm4__chr21 10.36 Mbp_chr21_10364477 TCCTCTGCAGGACCTTCTCTTCCCCTCTTTCCCTCCCTCCTGAGGTGAAAAGGAGGGGAGGAGACCACGGAGCCCTCTGT
GTCAGGGAAACCTGGCCCTTCCTTTTGAGCGTTCAGGGGACTC
mm4__chr5 73.30 Mbp_chr5_73297450 AGGAAAGAAGCGGGGTAAGCCTTTTGCAAAAGCTTTCAATTTTCTCTAGAACAATTTACAAGAACCCACTAGGGAGGGGA
AGTGGGAGCCTGAGGGGAACCCACTAGGGAGGGGAATTGGGAGCCTCTGTT
mm4__chr2 241.32 Mbp_chr2_241315785 CAACTGCCGTGGGAGGAGCACGGCGCTTGCGCAGAGGCGGACAACGCGGACGACTCTGGGCTTGCGCACTCGGAAGCCGG
CCGCATGGGGGAGGGGAAGAAGGCCAGGTCGCACTGAGCCAGGAAGCAGTT
mm4__chr17 81.51 Mbp_chr17_81512246 CCATCCACTCACCTGGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCGTCCCCAGCAAAACCAG
CTTTGCACATGCCGGAGCCATTGTCAATGACCAGCGCGGCGATCTCTTCTTCCAT
mm4__chr17 47.85 Mbp_chr17_47849274 CTCATACCAGTGCCAGGCAACTCTCTGACTGCTACCCATCTTCCTCTCCATGCTAGGGGAGGCCCAGATAGAAAGTTGGG
AGGGAATGGCTTC
mm4__chr9 131.53 Mbp_chr9_131530171 GTGTGTGGAGGGCCCTAAAGGCCCCAACGTGGGAAGGGAAGATGGGAGGATTTCTCCAGGATCACAGTTGTGGCAGGGCA
GGGCAGGGCTCCCCACAGTGCAACAGGCTTCCT
mm3__chr7 68.00 Mbp_chr7_67998279 TCCAATCCCAACCAAACCGTAGAAGTGTCCAAAGATGGAGGGGAAGACAGAGAGGAAGTGGGAAATAGCAGATCAGGCTA
CAGGTGAGAGGT
mm4__chr15 101.16 Mbp_chr15_101163245 GCACCAAACCGGCTCTATCTAGAATGATAAGGAAGTGTGTGAACTCCAAGCCCCAAGGACAGGCACCCTCTTCCCCTCCA
AGGTGAGTCCAGCCAGGTGGCAGTTGGATGCAACCAGAGAATGGTAGCAGAGGCAGGG
mm4__chr7 76.36 Mbp_chr7_76359847 ACGAACGACAAATACCGGCTGGCCACAGTGGTTGATTTCCAGAAAGGATGCAGCTGTCTGCTGGCTGACCCTGGAGAACC
CCTTCTTCCTCTCCAGGGTGGAGTGCTAAAGATTGAGTTCAGCTACCAGGAGCC
mm4__chr19 55.34 Mbp_chr19_55343509 AGTAGAAGGGTCCTGTGGGGCTGTGGGCTGGAGCAGGGAAGCCCACCCAGGCAGGAGGGGAAGAGGGAGATCTGGAAGAT
GGATCGCCAATAGCCAGC
mm4__chr8 104.73 Mbp_chr8_104732280 GCACGGAGGTGGTGTTTACTCACTCGTGTGAAGAGGACATTTTAACTCTCCATCTTCCCCTCCAGTGTGCAGTATCGATT
TTCTGCTATAAGGAGACAGAAAACCTTTCTTCAGAGTATCTTTGGAGGCAGAGTTGGCTG
mm2__chr2 27.37 Mbp_chr2_27373995 AGGATGTCCTGGAGGTGAGGCGCTTGTTCAGCACTGCCCCTTCTTCCCCTACATCCTGGGGTCCTGGGCTTGGAGAATGA
TTGGAACCAGCCAGTATGATGAGCTGTACTTCTATCCCTATCCCCAGGCTGTAGCTG
mm4__chr8 70.40 Mbp_chr8_70402448 GCTCCAGTCCCCGATTCCCGCCCCTCGGTCCCGCAGGCCCCGGAAACCACTAGAGGGAGGGGAAGAGGGAGGGGCCATCT
AACCAGGGACCGAC
mm4__chr1 105.86 Mbp_chr1_105862469 AGGTAAACGCCACAGGAAGGTGATTCAATCATTGTTTCTCACTAACAAAACTAAAGGTTCCCTACTTTGGAAGGGAAGAG
GGAGTTTCTGTCATGTGGCAGATTCCA
mm4__chr3 47.84 Mbp_chr3_47840860 AGGCCGACTGAGGTGTAAAGATGGTATCAATCCTTAAAACGTCCCTTTTCCCCTCCAGGGTTGGGGTCTGCTAGGTCCCC
GGAATGAGTTGTTTGACGCAGCCAAATACCGA
mm3__chr1 229.43 Mbp_chr1_229433001 GCAGCCTGACCTGGTGTCGGGGGCGGCCCACGATGGACGGGAACACGGCCCTAGGGGCGTCATCCCCGGCGAAGCCGGCT
TTCACCAGGCCGGAGCCATTGTCGCACACGAGGGCGGTGGTCTCGTCTTCGTC
mm3__chr1 215.08 Mbp_chr1_215081844 TCGCCTATTACGACGACAGCAACGTCTGATCTCCTGGCTTTCTTCCCCTCCTTGGTGGGTTCCGTTCCGTCCCCCTGTCG
CTGCGAGTTTGGCTGTCCCTACG
mm4__chr12 118.04 Mbp_chr12_118039557 ATGCAAACGTGTGTGCACAGGATTATACAAGTAGACAGAAAAATATGGAAGGGTATATACTCCTAACACAGGGTACCAGG
ATGCAGGGGAAGAAGGGAGGATGGCAACAGCAGGGAGGGCAAGAGAGAGA
mm4__chr4 45.03 Mbp_chr4_45034172 GAGGACAAGGAGGGCCATTCAGCCAGTGGGCTGCAAAAGTGAGACTGTCACCACAGTGCCCTCTTCCCCTCCCTGGTGCT
GACAGCTTGCAGTCATGCTTGCGCTGTCTGCAAAATATCACCTTAGGGGAGTCTGTCTGC
mm3__chr1 66.76 Mbp_chr1_66756775 ACAAAGTCCACAGACCCCTATCTACTTCCATAGCACCATCTTCCCCACCATCTTGCGGGCATCATCCCCTCCAGCTATAC
TCAGTTATTCCTCAGTGCTCTATCTGGCCCCCAGCCATTGCCCCATGTTGCTTC
mm3__chr19 35.53 Mbp_chr19_35534553 CCCCAGGCTTTTCTCCAACCGGGGGACCCCACCCCTACAGTGGAGGGGAAGAGAGTGCAAGGCTGGGAGTGCTGGGAGAG
GAGGACCATCTG
mm3__chr6 33.66 Mbp_chr6_33662727 GAGGTCAGAAAAGCCCTGCTTCTGGCACCCCGGACTCAGCCTTGGATGCCACCCCTCCCTCTTCCCCACCATCCTGGAGG
GTGGGATATTGACCCCTACCTCCCTCTG
mm4__chr6 44.13 Mbp_chr6_44133255 CTTTGCAGAGACTCCCTGGGACCAAGTGAAACACATAAACAACTCCATCCTCCCCTCCATCTGAGGGCTTCTGCCTCTTG
CCCTGTGGTTGAAGTGTT
mm4__chr7 4.30 Mbp_chr7_4298332 GAGAGTAAAGCGGTGCCCATAACCCTCCTCCCTTCCATCCTAGGGACAGAGGCAGACATTCATCTGTGAATCTGCTGGCT
CTGATAGTGCT
mm4__chr16 19.17 Mbp_chr16_19174521 GTTTCAGCTCAGTCCTTGGCCTGAGATTCCAAAAGTCTCTGGTGTCCCATCTTTCCCTCCAAGCTGGGGGCTCATGCCTC
AGGCCTCTCATTTTCTCT
mm3__chr20 62.59 Mbp_chr20_62590954 ATTCTGGTTCCCTGAGCAGCAGCCCGGGGTGTCAGCAAAGTGTGGGGCATGGACCCCGTCCCCTCTTCCCCTGCATGCTG
GGGTTCCCATGTCCCCAAGTGCATTGC
mm4__chr12 116.53 Mbp_chr12_116534154 TCCGGTTACAACTAGACGCGGCCCCTCCCCCACCTGCCTCCCCCCTTCCTTCCCTCGATCGTGGAGGGAGCGTTCTCTGT
GCCTTCCCAA
mm4__chr5 6.18 Mbp_chr5_6180963 GTGGTGCTCCCTCCTCTTTCCCTCCATCTTGAGGAAGGTGTTGCCCTCCTCTACTTCTCTCTTGTAGTGCTCCCCCTCCT
CCCCTCCATCCTGAGAGAGGTGTGACCCTCCTCTCCCTCTCTCCTGTAGTGCTCCCC
mm3__chr5 6.18 Mbp_chr5_6180838 GTGACCCTCCTCTCCCTCTCTTCTGAGGGGNNNNNNNNNNNNNNNNNNNNNNNNNATCCTGAGGGAGGTGTGAGCCTCCT
CTCCTTCTCTTCTGTGGTGCTCCCTCCTCTTTC
mm3__chr5 6.18 Mbp_chr5_6180769 GTGACCCTCCTCTCCCTCTCTCCTATAGTGCTCCCCTCCTCCCCTCCATCCTGAGGGAGGTGTGACCCTCCTCTCCCTCT
CTTCTGAGGGGNNNNNNNNNNNNNNNNNNNNNNNNNATCCTGAGGGAGGTGTGAGCCT
mm3__chr5 6.18 Mbp_chr5_6180592 AGGTGTGACCCTCCCTTCTTTGCCTCCGGTGGTGCCCCCTCCTCCCCTCCATCCTGAGGGAGGTGTGACCCCCTCTCCTT
CTCTCATGCAATGCTTCCCCCTCCTCCCCTCCATCCTGATG
mm3__chr17 3.92 Mbp_chr17_3915975 CCTCGTCCCCATCTCAACAGCGAGTTGGCAGGATTTTCTATTCTCGCACAGTGGCTTGCCAGGAAGGCCTTCCCCTGGAT
GGAGAGGAAGAGGGGCTCGCTGGTGTCACGCAAGGGATGTCAGA
mm4__chr2 71.83 Mbp_chr2_71827637 GCTGGGGTTTCTGGGAGTTTCCCGGATTGTCTCCAGTTTGCCTGTAATGCGCCCTCTTCCTCTCCATCCTGCTGCCCCTC
CAGCTCCCTTCCGGCAAACCCTTGGGAAGGTGCCAGAGTTC
mm4__chr3 143.86 Mbp_chr3_143855297 GGAAAGCCGAGGACAGCATACTCTGAGACACACTTGCTGGGGTAAAATCTATCTGCACAGTAGAATCTACTTCCAAGTAC
TCAACCTCTCTATAGAGGGGAAGAAGGGTTGCCTTTTGCAGAGACAGCAG
mm4__chr15 62.59 Mbp_chr15_62591612 TGAGTCACACCATGGTCAAGGTAAAGACAAAACTTGACAGTTTTAAGTAACCTGATGGAAGTGTACAGACTCCTTCCTTC
CCACCATTGTGGGGCAGCCTCTGGCCAGATGTGATCT
mm4__chr17 57.12 Mbp_chr17_57121995 TGAGCTGTGGCCATTGTCAGGTGCCCATCTTCCCCTCCATCCAGGTTCTCGCCTGTGTGGTTAACNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNCCTCATAAGGCAATTTAATCATGGAAATGCCCTCTGC
mm4__chr16 74.40 Mbp_chr16_74397860 GAAGGAAACCTCAGGTCGGGCTGGAGAACTCTTTGCTGAGCTTGTTCACTGCTCCTGGCATCCCCACTATGCAGAGGAAA
AGGGGACTCCCCAAGCAGTGGGATCCCC
mm4__chr3 12.07 Mbp_chr3_12070309 TCATTGACAGTGTCTCCGGCATGTACAAAGCTGGATTTGCTGGGGACGACGCCCCCTGAGCCGTGTTCCCCTCCATCCTT
AGGCGCCCCTGGCACCAGGGCACGATGGTGGGCATGGGTCAGAAGGACTC
mm4__chr11 69.75 Mbp_chr11_69753548 CCTGTGGTGGATCCTACGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGTCATGAGCAGTGACTGTGC
mm4__chr19 12.99 Mbp_chr19_12985900 GAGATCTGATGCCAGCTGGGGCACAGAACCTCCTCCCTCCATCTTCCCCTCCATAGGCTGGGTTTGGAGGAGTGGCCTCT
AGATGCAGC
mm4__chr19 40.40 Mbp_chr19_40398532 TGACAAGACAGAGGGCAAGGCTGGCCCACGATGGCGGGGAATGGGGCTCACGGCGCAGAGACCGGATCGCTGGGGCAGTC
CAGGGCCGGGGCCGGGCTAAGCACGCGTACCAGCTT
mm4__chr10 86.34 Mbp_chr10_86341339 TGCACTGCCCACTAAACAGTCCCGGAGGATGGAGGAGAAGATGGGACTAGGATGAGGCCCTGGTGGGACCCTGGGTACTT
A
mm4__chr15 28.66 Mbp_chr15_28657610 CACCCTCTTCTCCGTGTCACCCTTTATCCCGGAAGAGAAGTCTGTTCACCTCTTCCCTCCTCCCCTCCATCCCAGGGCCC
TGTGGCCCCGTCACCCTGTTGGACCATGGCTCACAGCCTGTTCTCTGCCGGATCCAG
mm4__chr15 22.56 Mbp_chr15_22563372 CACCCTCTTCTCCGTGTCACCCTTTATCCCGGAAGAGAAGTCTGTTCACCTCTTCCCTCCTCCCCTCCATCCCAGGGCCC
TGTGGCCCCGTCACCCTGTTGGACCATGGCTCACAGCCTGTTCTCTGCCGGATCCAG
mm4__chr15 20.44 Mbp_chr15_20436985 CTGGATCCGGCAGAGAACAGGCTGTGAGCCATGGTCCAACAGGGTGACGGGGCCACAGGGCCCTGGGATGGAGGGGAGGA
GGGAAGAGGTGAACAGACTTCTCTTCCGGGATAAAGGGTGACACGGAGAAGAGGGTG
mm4__chr16 54.20 Mbp_chr16_54203201 CCACTTCTTTGGGGAGTGCTCCACTGTTTTTAGAATACGGTTCTGGCTAACACTAGACCACCCCATGAAGGAGAGGGAGA
AGGCCATGACTCTGTCATCCCCTGCTAATCTTAGCACCAGGGCTTGAGTAAC
mm3__chr16 26.92 Mbp_chr16_26917228 AAAGTCCCCTGCCTTCCATGCTGCAGGGATCGCCAAGATGGAGGGGCAGAAGGAAGGGCATGTGCAGTGCTTGGCTGAGA
CAGGGAAGTGATGGCCACAT
mm2__chr3 184.16 Mbp_chr3_184156562 GCCAGTGGAGAGACGTTAGGAAGGGCCTGGGGCCTGGGTGGGATAGCCAGGAAGAGTTTCTTGGTGTTCTCTATCTTCCC
CTCCCTGGTGGGGTCTTCCCTTCCACCCAGCTGCAGAGATCTGGACAGGCACAGTTTGGA
mm3__chr19 54.14 Mbp_chr19_54141815 TGACTACTCCAGGGTTCCCTGCCCTGCACACGTAGAGTGGGAGCGGCCCGTGGATCCCAGGGAACTGTGCTTTTCATTGT
AGGCCCCCTCCCTGGAGGGGAAGAGGGCAATCTCCGCTGGTATCTCAGAAGTCT
mm4__chr11 35.14 Mbp_chr11_35142768 CAAGTCCTTGTGTGGGAGGCTGGCAGCCAGTAGGAAAATGTTCCATTTGTTTTTTCCCAAATTTCCCCACAAAGGAGGGG
ATGCTGGACAGCATGAAATTCTTAGTTCATGACAGATTCCCTTCCA
mm4__chr15 40.36 Mbp_chr15_40357831 CCCAGGTCCAAGTCGAAGTCCAGAAGGATGGGAAGAAAGACCCAACCTGATGCCTCGCCCCACTGGGGAGGGGAGGAGGG
CGCTGAGCGAGCCGGGAACCTCGCAGGCCTGAAG
mm4__chr9 81.82 Mbp_chr9_81822329 TTTTAGCTGGAGGGTGAGCCCTCTCTGCCAGCCCTTCTCCCCCTCCACCTTTGGGGNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNAAGATGCTGAAATCTGCTTTGTGTGA
mm4__chr5 6.18 Mbp_chr5_6180654 CCCCTCTCCTTCTCTCATGCAATGCTTCCCCCTCCTCCCCTCCATCCTGATGGAGGTGTGACNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNGAGGGAGGTGTGACCCTCCTCTCCCTCTCTC
mm2__chr15 34.79 Mbp_chr15_34794694 GGGAAGTTTACCTGGTGCCGCGGGCGGCCCACGATGGACGGGAAGACAGCGCGGGGCGCGTCATCGCCCGCAAAGCCGGC
CTTCACCAGCCCAGAGCCGTTGTCGCACACCAGGGCGGTGGTCTCCTCGTCGTCACACAT
mm4__chr5 6.18 Mbp_chr5_6181207 TCCTTCTCTCCTGTGGTGCTCCCCTTCTCCCCTCCATCCTGAGGGAGGTGTGACCCTTCTCTCCCTCTCTCCTATGGTGC
TCTCCTTCCTCCCCTCCATCCTGAGGGAGGTGTGCCCTTTCCTCTCTCC
mm3__chr10 124.53 Mbp_chr10_124526823 CCAGAAGTCCCATCGTTGCTGCCTTTGTGGTTAAAGTCCGCTGAACCCAAGCGCTCTCTTCCCCTCCAGTGTGTGGTGGG
GAGGTGGGGAGGGGACCCAGGCAGATCTGGGAAGCC
mm4__chr1 28.86 Mbp_chr1_28856882 GGTTGGATGTGTGACCCTGTCACCTGCTCCGCTTCCCCACACCACTGTGGATGGGAAGAGGGATGCACTGTGGCTTCTGC
AGAGAGGTGAGTCCAGTTGCATGGGGATG
mm4__chr4 151.58 Mbp_chr4_151577808 TGCTGAGCTCTTGTTCAGGAACAACCACCCCCACTGTGGAGGAGAAGCTGGTTGTCCTACTCAATCAGCTCTGTTCCATT
CTTGCCAAAGATCCATCCATTTTAGAACTCTTCTTCCACACTAGTGAAGACCAAGGCGC
mm4__chr15 88.26 Mbp_chr15_88256806 GGGTTATCGGAGCGTCTTCCTCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTCGCGGCCTCCTCCCC
CCGCCCCCCGCCCGGGCTGGGAGCGGCAGGAGAGCGGAATCTC
mm4__chr15 69.39 Mbp_chr15_69393658 CCTCTCCCTCCTGCTCCATCCGGCTGCAGTTGGGCAGGAGCGGGAGAGAACCACCTTCCCCTCCAGGGAGGGGAAGACCT
CCTGGGGGAGACCAGGAACCTCAGT
mm4__chr9 130.34 Mbp_chr9_130343907 GCTACAGGGTTGGGACTGAGCCCAGCACCCTCATGGGGACTCCCTGGGCCCTGCAGTGACACCATCTGCCTCTTCCTCGT
GGGGTGGGGTGGGGCGTGAAGGCCCAGAGCCTGGACACCCACTTGGTGGTGTGTGTGGT
mm4__chr4 80.18 Mbp_chr4_80184143 CGGTACCCAACCCTCTCTCTTTCTTGACCTTCCTCCCCTCTGCCCCTTCATCCTGGGCGCGGAGGGCGGAACAGTTTCTG
GACCAGAGCACC
mm4__chr1 158.08 Mbp_chr1_158079231 GCCCCTCTTCCTTTCCTTCCTTCTAACTTGAAATCACTGAATTTGCGGGGAGCCCCCAAGATGGATGGGAGAAAGGAGAG
AGGAGCTGAGATGAGGGAGT
mm4__chr10 104.15 Mbp_chr10_104152797 AGGCTCCTAGGAAGTGATGTTTACATTTCACCACAAGGGAGGGGCAGATGGAGGGCAGCCCTTGCAAGGTGTTCTGGGCT
CTGTACTCTCTTAAACCAGAGATGCTCCCACTTGG
mm4__chr9 131.48 Mbp_chr9_131482040 GATTTCTGGCCTGTGCATGGGGCGTGCATGAGCAGGAGAAAGAGAAAAGGGGTAACAAGGGAGGACCCAACGCTGGGTGG
GAAGAGGGATTCAGGGCCTCCTCTGAACTGGTGTCAGCAGCCACGTAGTCTGGTTTGG
mm4__chrX 72.02 Mbp_chrX_72017129 AAACAACCCACCCCAGAAGGGTGCACTCATTCATCATGCCCCTCTATATAGGGGAAGACGGGCTGGGTAGCAGGGCCTAT
TG
mm4__chr8 47.48 Mbp_chr8_47483006 AAATTCCCACTTGCTGGCTGCCCCCTCCCCAAGGAAAGGCCGCCCCTCTGCCCCTCCACAGTGGGTGGTGCCCAGAGTCA
GGGGACAGGTCTG
mm4__chr12 52.81 Mbp_chr12_52809350 AGGGAGCTGGAGTCCTTTCAAAAAACTGTGAATCCCAACCAGCAGCGTCACAGATGGGGGATTCCCTTTCCCTGGATGGA
GGGGAGGATGGAGCCCTCACCGCATCATAGAG
mm4__chr1 110.21 Mbp_chr1_110209706 GCCAGAGCTACGGTTTCCTTCCATCCGGCACAAGAAGCGCTTCTCTGCCCAAGGCCACCCCGTCCTCCATTCCATCGCGG
GGACTTTGGTGGCTGGGACAGAGGCGGAGAGCATCAAGACCAT
mm4__chr2 163.35 Mbp_chr2_163348874 CTCCTCCTTTCCCACTTGCAAGGAGGCTTCACCCCCTCATCCCCTCCAACGAGGAGAACAGTATCAAAGGTATAAAAGGG
AAATAAATGCTCCAAGATAACTGTATATTTTGCCACACAAATTTGAACTGGGTTTCGCGT
mm4__chr7 100.49 Mbp_chr7_100486785 AGGGTTGCTGACACCTCTTGGGGTGGAGAAGGGGGATGCCCAGGTCCGAGGCCCTTCCTCCACTCCATCGTGGCCTTCTC
TCCCCAGCTCCAGTAAGG
mm4__chr14 58.65 Mbp_chr14_58646817 GGCTAGCGACTCCGAGTACTCGGCCGAGTGCGAGTCCCTGTTCCACTCCACCGTGGTGGACACCAGTGAGGACGAGCAGA
GCAAT
mm4__chr22 50.44 Mbp_chr22_50438048 CTCGGAACAGTCGCTTTCCTTGCCCCTCCCCGTCCTCCCCTCCAGCCCGGGGCTCCCACATCCCGAACTAAGCCCCTCT
mm4__chr19 1.99 Mbp_chr19_1991613 ATGACCCGGACAAGAAACCCCTCCCTGTGGGAGTCCGGGATGCCCTGCATCTGACCTGCCAGGATGGAGGGGGAGAAGGT
TCTGGGTCCTTTCCAGGATGCCGGACCCCCAAAGCCCATTGGAGGAC
mm4__chr19 18.60 Mbp_chr19_18598987 AGGCTGAGGGTCTGGACTAGCTGAGCCTCGGCTGAGGGGTTGCTGCCCACCCACCAGGCCCCCACCTTGGAGTGGAAGGA
GGGCAGACAGGACAGTCTCAGCCCTGTGCTGAGAAATGCCAGC
mm4__chr15 87.95 Mbp_chr15_87953912 ACACATTGTCTGCAGGCTGTGGCCTCCTTGGGAATAGACCACTCCACTTTCTCTGCTTTCCCTGGGAACGAGTTCTTTCT
AGCCCCCTTGCCCATCTGCCCCTCCTCCGTGCGGGTTATCGACCTCCATTGCCAGC
mm4__chr7 32.36 Mbp_chr7_32361591 CTGGGCCTTTGCTCTCCTTGTGCCCTCTGCCCTTCCACCCTGGGGGAAATTGTGATCTCTGCCTCTTCAAGGTCCACCTC
AGCTGCCCTTGGTGACACCTTCCTTAAGTCACCTGGGCAGCA
mm4__chr13 27.78 Mbp_chr13_27777745 TGGTGAGAGAATACCTGGACATTTAAATCTATCAAAAAGGAGTAAATGAGCATTTCAGATACATCTATCAAGAAGAAATG
CCCCTTCTCCCCCATCATTGTGGGGCTTTGTCAGTCGCCTGTCAGATGAATGTCC
mm4__chr1 15.62 Mbp_chr1_15616534 CTGCACCCACAGCCATCTTATTTGCTCCTTCTCTCCTGTTAAAATGGAAAAGGGCTCCCTACCATGGAGGGGGAGTGGGA
GTAAATGGAAAGATGCTAATCTGGCA
mm4__chr14 60.25 Mbp_chr14_60245647 TCTCTCAGGTCGCAGGATGAGCCACAGTGGTAGCATAAGACTGCTGGCTGCTCCCCTCTGCCCCTCCCTAATGGGGCTGC
CCAGTGATGTCACACA
mm4__chr22 48.61 Mbp_chr22_48610583 GACCACCAGGAGGGCTTTGTCCCCAGCATGGAGGAGCAGAGGGGCAGGCTCGCAGGCAGCTCGGGACCACCTGGAGGGCT
TCGGGCCGTGTTGAGCTGTCAGCGTTG
mm4__chr17 79.79 Mbp_chr17_79791997 ACCTCTATCCCAGCCAGGTGGGTTCCCCAGTTGGGGAAGGGCCTTTGCAGGAACGGTGCCCCCGCGATGGGGGCGAGGAT
GGGGGCGGGAGGACGGGATGAGTACTGAGAGAAGAGGGACGGATCCTCTCC
mm4__chr17 36.50 Mbp_chr17_36501669 CACACATCCTGAGGGCCAAATAGGGCAGCTATAGGCCACTCCCTCAGCCCCGACCCCTCAACTCCCCACCATGTATGGGA
AAAGGGAGATCCAATCCCAGCCCTTAGTTTGGGAA
mm4__chr10 131.14 Mbp_chr10_131135295 TCTACCTCTGGCTTATTCCGCCCCGCCCAGCTTGGGTGGCCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNTCCCTCCATCTTCCCATCCAGCCTTGGGCCACCTTCCCAGTGACCG
mm4__chr1 164.85 Mbp_chr1_164852508 TCCCCAGTGTTCTAGCTGGAACACCAGGCCTCCACACCTAAGTCTGTGCCCAAGTCTTTAACACCATCTTCCCATCCATA
GAGTGGGATGGAACTCCTTGTCTGCCTTTTGACATGTTACTCTAGGGCCATCACATGC
mm4__chr11 124.85 Mbp_chr11_124845424 CACCTTCCCACCAGTCTGACTCTAAAGCCCCCAGCCCTCAGTAATGCTTTGCACCATCCCCCCCTCCACTGTGGGGGCCA
CCAGTACTGGCAAAAGGGGGAGCATAAATAATTTTCTCCAAAGGAGCTTGTGTCTAGA
mm4__chr10 11.38 Mbp_chr10_11381496 GCACAGAGAAAGCAGTGTGCTTCTCCTATTTAGACGGGGGCACCCCCGGAGCATATGTGCAGAGCCCTGAGGCTTCCCAC
GAGGGAGGGGGACAGGGAGGAGAGAAGGCAGAGAGACAGACGG
mm3__chr14 84.99 Mbp_chr14_84991950 GGACGAGTTGCTGAATTGTTCAGAAACTGTGTAAATAGAATGTGTTGAAGTTAGTATATAATTTTCTACCATCCGCCCCC
ACGGTGGAGTGGAAGCAGGTAATAATTTTTTGTGACGGAAAGATACGTTTAATGACC
mm4__chr9 22.82 Mbp_chr9_22819991 AGCTGTTGCAATTACAAAATGATCAGAACATGTCTACATTATCAATCTAACTTACCCTCTGCCACTCCATTTTGGGGTTA
AAATAGAATTTAAAGAGTCTGAATATTTTGACATGATAGAGGGTTAATCTTTCAGG
mm4__chr7 151.43 Mbp_chr7_151431361 TGGGGACGTGGACCTCTTATGCCCAGGAATCCCACTGCCTGGGCCGAGTATCTCCTTCCAGGACAGACTTCTCCCTCCCC
TCTGCCCCTCAAGGGTGGGGTGTCCCCTCTAGATTCAAGCCCACCCCAAC
mm4__chr14 21.03 Mbp_chr14_21032659 TTTCAACACCCAGCCCAGAACTAAGTTTTACCCCACATCACCTCCATCATGGGGCACATGGGACAGAAGAGCTTACTGAC
TGCTAAGGGTAGCTCAGGAAGATGGCCAGATGAGGCAGGCTGCCAGGATTCCAAGAGCA
mm4__chr1 156.69 Mbp_chr1_156691446 GGAACGTAGGCTGTGGGAAAGACTGACATTTCTTTCCACACTGAAGAACCCAGGCCCCTCGCTGGTGAGGAAGAGGGTGC
GGAGGGGTCTGGCTGACACCCGTTGGGACTAAGCTGGTT
mm4__chr10 129.46 Mbp_chr10_129461459 CCTGCCTTTCCTTCTCTGGGCTGTAGTTCACTTTCTAATAAAGGTCCTTTGGGTTTGGCGGCTTTGTTCTGGGCCTCCTT
CACCTGCATCGTGAGGAGCACCAGCAAAGGCCGCCTGACACTGCCCTCACCAAGAA
mm4__chr19 57.04 Mbp_chr19_57035735 GGGATTTCCACAGGGACAGGTAGAGGTGCAGCTAGGAGAATCCCCAGGATGTGGGGGAAGCTGGGCAGAGCACAGATCCC
TGGGGTTTA
mm4__chr4 7.39 Mbp_chr4_7394617 TGCTGGGGTTTAGAATGAGGGGGTGGACTGGTGGGGACTCTCCCCTCGAGGGAGGGGGAGGAGGAAGCAGTCTTGCTGGG
TGATTGGG
mm4__chr20 62.51 Mbp_chr20_62514784 ACTTGCTTTTGTCCGACCCTGGCCCTGCTCTCTCTCCATCGTGGGGGCCTGTCGGTGCTGGGGAGACTGTGGGCTGCTCG
GTGTTGGGACCCACACAGGTG
mm4__chr11 60.93 Mbp_chr11_60930503 CCTTCAATCCAGCCCACAGTTCAGCATGCACCTTGCCAAACTGAGCCTATACTCTCTTCCCCAGGATCAAGGTGAAGAAG
GGGCTGCATGTGACAGCCGCCCGCCCAGCCCAGCCCACACTCTGGACTGCCAAGCTG
mm4__chr18 12.25 Mbp_chr18_12245840 CTGGCTCTTTCTGGTCAGGGCCACCCCACCCAGCAGGGTCAAAAGAGGACCATTTCCATGAGCTGAAGGGAGGCACCAGG
GAGGGCAGAGGCTGGAGCTCCAGGCTGGAGGTGAAGATGGCAAAACCCGGGCATGAGGAA
mm3__chr12 56.61 Mbp_chr12_56611570 TCAGAGGAAAAGAGGCATTCCCTTCTTTCTAGAGTTGTGCATTAAACTTGTCAAGGTCAATAAATGTAGCAAACTAGAGC
ATTTACCCATGTTGGAGGGGAAGACGAACTTCTTCTGGGGTAGCAATACGTCTCC
mm4__chr16 20.62 Mbp_chr16_20623403 TCAACACTCTCAATGCCCCACCCTCGTCCTCACCATAGTGGGGAGACTAAAGTGGCCAGGGATTTGCCTTAGGT
mm4__chr3 73.74 Mbp_chr3_73737265 TCCACGTGTGTTGGCAAAACTGAACATAAGCTCTCACCCCAAAGGACTCCGCGATGGAGGGGCAGCAGGCAGGTGAGTGA
AGGATTCTGGCATTCATGGGTCCC
mm3__chr10 127.13 Mbp_chr10_127134952 AGGCTAGTGTCAGCAGGAGAGGTAGGCGCTCAGCCTATCTCCCACTAGGGAGGGGAAGAAGAAAGGAAGGAGGCGGCTGA
TCCCTTGAGGCTGGGGATGTGCTCAATGGA
mm4__chr20 50.24 Mbp_chr20_50243253 GGCACAAAGGCCCATTGTTCGGGCCCACGTTGGCGGGGAAGCGGGCGGGGAGCAGCCGCCTTTCACCCAACCCGCTTGTG
GTCGGCGGGGACTGAGGAGCGATTCAC
mm4__chr12 109.78 Mbp_chr12_109776531 AACACAAGACTCCCAGCCTGGCCTCTCCTGAGCCAGCCTTCCACCCCTCAATCCTGGGGCTTGGGTCTCCACCAGTCAGT
GCCTCTCCCATCCTGACCTCTGGCTCCATCAC
mm4__chr17 45.45 Mbp_chr17_45452137 ACCTAAGGGTCCCATGGTGATGGGATGCCATCTGCCCCTGGATGGTGGGGGGAGTGGGAGGGGCTAACAATGG
mm4__chr5 6.18 Mbp_chr5_6181146 CTCCACACCTCACCACCATCCTGAGGGAAATGTGACCCTCCTCTCTTCTCTCCTGTGGTGCTCCCCCTCCTCACCTCCAT
CCTGAGGAAGGTGTGACCCTCCTCTCCTTCTCTCCTGTGGTGCT
mm3__chr1 33.97 Mbp_chr1_33965180 TCATCAAGCTTCCCCTTGGTAAACCTTATGGAGCTTGAATCAAAGTCACCCAGGATGGGGTTAGAATTCTTCTCTCCACT
CATCCTCTTCCCCTCGACCGTGGAGTGAAGAAGGCCCTCCTGCTTCCCACTTTCCAC
mm4__chr5 2.80 Mbp_chr5_2801820 GAATGATGAGGGCGGAGAGGGTGACGGAGTGCATATTCGTGTGATTACAAAGAGAAGCTCAAACAGTAAATTTGACAGCT
ACGTTGGAGGGGAAGAGGATAAGAGTTATAAAGTGACAGCTGTATTCCTTCAAAGTGC
mm4__chr13 43.97 Mbp_chr13_43971040 GAGACCTGTTTGCACCAAGCAGCGGCCCTGGGCCAGCGCACCATCTGCCACTACATCGTGGAGGCCGGGGCCAGACCAGC
AGGGCGACACTCCCCGGCAGCAGGCTGAGAAGGCTCAGGACACCGAG
mm4__chr11 46.38 Mbp_chr11_46379512 GAGACCTGTTTGCACCAAGCAGCGGCCCTGGGCCAGCGCACCATCTGCCACTACATCGTGGAGGCCGGGGCCTCGCTCAT
GAAGACAGA
mm4__chrX 109.83 Mbp_chrX_109825131 GGACCTCATATGCAGCAGCTGCTGCTGGGCCGGGGGTAGTCTTCACCCAGAAAGGCCCCATCCCCTCTACGCCTCCAACG
TTGGGTGACTGTCCTCCAATAAGTCCACCATGGAGAAGTCT
mm4__chr10 129.19 Mbp_chr10_129192607 GGCTGGCCATTTCCTTCTTGTCCATCAGGCCGTCTCCACCTCCATCTGGGGGGCAGATTGGATCTTGCCAGGTTTTAGAT
CTCAAGCCCGACATTTAGTAGCACTCAATAAAGATTCGCTAGGACATTGCAACAGT
mm4__chr2 239.48 Mbp_chr2_239482524 TGGGAAGAATTTCACCGGGAATCCCGATGCTGCAGGTCTGTCGAGGCCGGAGCCTGGGTGTGCTCAGGATGGGGCCCTCC
TGCAGGGAACCATCTTCAGCACCATCCTGGGGGATGTCGTGATGTTTTGCATATCGA
mm4__chrX 109.82 Mbp_chrX_109817510 AGACTTCTCCAAGGTGGACTTATTGGAGGACAGTCACCCAACGTTGGAGGTGCAGAGGGGACAAGGCCTTTCTGGGTGA
mm4__chr10 120.59 Mbp_chr10_120591327 CTGGTCCTTCCAAACTGGCTTTGGCACGGTCATGTGATTGTCCCCTGGATGGAGTGGGAGAGGGGGAGGCTTGAATTGAC
TTTCATCCAAAGTCAAGCCATTGATTTTTTAAAAATTATAGCAGTTACATTGGCAAGCA
mm2__chr2 96.13 Mbp_chr2_96131842 TGAAAGGTCTCGTGCTGGACGCCCTCCACCTGCGGTAGGCCTTCCTGCTCCAACCCCTAAGCCAGACTCCCACCCAAACC
TCCTCTGCCCCTCCCTCGTGGGGCTCTTCCTGCCTCCTGAACCAGACCATCAAGCCCA
mm4__chr6 34.55 Mbp_chr6_34554131 GGCGGGACCTCACCTGTTCCTTCTTGCCCTCCACCTTGAGGGTCTGTGTCCCCCTGTCAGACTAAATTAACTTCCCCGGG
TTATACTCAGGCTGCAACAAGTCA
mm4__chr5 1.10 Mbp_chr5_1102563 CTTAGACTCAGCCTCAGCCGGAAGCAGCTGACAGGCAGGAGGGGCTGTGAAGCTGAAGCCAGGTCCGACAGCCTCCGCCC
CTTCCTGCCCTCCAGCGTGGGTGGTCCGTCCAGCTACCCAG
mm4__chr1 57.88 Mbp_chr1_57883441 TGCAAAGCCAGACTGCATTGTATTCTTAAATTGGGCCTGAAATAGCCACACGATGAAGGGTAATAAGGCAGAGAGCACAA
CCCAGAGTGCCTGGTGCTGTGCAAAATT
mm4__chr1 208.02 Mbp_chr1_208016929 AGGCAGAGGAGAAGTCAGGTACCGTCAGGCTGGGGAGCCTCCCTTCTTACCCTCCAGTGTGTGGCACTGTAGGCTCTTCT
GTGGGG
mm3__chr16 5.91 Mbp_chr16_5905903 CACTCCCCAAATGCCTCCTCCGGAAGCCTTCCTAATTTCCCTCTTTAGAATTCATCTCTTTTTCCCGACGATGGAGGATA
AGAAGGTTTTCAGCTCTCTTGTAGTGGGAATTGGCTGCCCAAAGCCCTACCTTAGG
mm3__chr1 112.00 Mbp_chr1_112004368 TCTTTCTGCCCATAGCCTGGAAGGGCACAGGCCCAGGATGGAGGGCAAGAAGGATAAGGGATTGCTGCCCTGGGAAGCCT
ACATGTGTTTCCTGATCCAGAGGTCTTCCTCAGAGGCAAACA

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.