CRISPOR

Micro-homology scoring of potential deletions

Guide: ACCTCGGCTCACAGCGCGCC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	(Wild-type sequence)
404.1	`AAGTGGCCAGGGCGGGGGCGACCTCGGCT----------------ATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`16 bp deleted → frameshift`
327.6	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCG----GCTATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`4 bp deleted → frameshift`
311.6	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGC-----TATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`5 bp deleted → frameshift`
298.2	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGC--------------AGCTCACCATGGATGATGATATCGCCG`	`14 bp deleted → frameshift`
296.4	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCG------GCTATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`6 bp deleted → no frameshift`
282.0	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC-------TATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`7 bp deleted → frameshift`
269.4	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC----------------AGCTCACCATGGATGATGATATCGCCG`	`16 bp deleted → frameshift`
255.2	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGC---------TATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`9 bp deleted → no frameshift`
247.0	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCAC----------------------------CATGGATGATGATATCGCCG`	`28 bp deleted → frameshift`
245.0	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGC---------------------TCACCATGGATGATGATATCGCCG`	`21 bp deleted → no frameshift`
191.1	`AAGTGGCCAGGGCGGGGGCGACCTCG--------------------------CAGCTCACCATGGATGATGATATCGCCG`	`26 bp deleted → frameshift`
175.0	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTC---------------------GCAGCTCACCATGGATGATGATATCGCCG`	`21 bp deleted → no frameshift`
172.2	`AAGTGGCCAGGGCGGGGGC-------------------------TATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`25 bp deleted → frameshift`
170.8	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGC-----------------TCACCATGGATGATGATATCGCCG`	`17 bp deleted → frameshift`
162.8	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGC------------------AGCTCACCATGGATGATGATATCGCCG`	`18 bp deleted → no frameshift`
154.8	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC-------------------TCACCATGGATGATGATATCGCCG`	`19 bp deleted → frameshift`
148.2	`AAGTGGCCAGGGCGG----------------------------CTATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`28 bp deleted → frameshift`
140.0	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGCC---------------------ATGGATGATGATATCGCCG`	`21 bp deleted → no frameshift`
133.2	`AAGTGGCCAGGGCGGGGGCG----------------------GCTATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`22 bp deleted → frameshift`
127.2	`AAGTGGCCAGGGC-------------------------------TATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`31 bp deleted → frameshift`
116.1	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTC-------------------TCGCAGCTCACCATGGATGATGATATCGCCG`	`19 bp deleted → frameshift`
114.8	`AAGTGGCCAGGGCGGGGGCGACCTCGGC-------------------------AGCTCACCATGGATGATGATATCGCCG`	`25 bp deleted → frameshift`
113.6	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGCC---------------------------------------G`	`39 bp deleted → no frameshift`
109.2	`AAGTGGCCAGGGCGGGG--------------------------CTATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`26 bp deleted → frameshift`
105.0	`AAGTGGCCAGGGCGGGGGCGACCT---------------------ATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`21 bp deleted → no frameshift`
103.6	`AAGTGGCCAGGGCGGG---------------------------CTATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`27 bp deleted → no frameshift`
96.0	`AAGTGGCCAGGGCGGGGGCGACCTC---------------------------------ACCATGGATGATGATATCGCCG`	`33 bp deleted → no frameshift`
95.1	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCA-----------------------GCTCACCATGGATGATGATATCGCCG`	`23 bp deleted → frameshift`
94.2	`AAGTGGC-------------------------------------TATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`37 bp deleted → frameshift`
90.3	`AAGTGGCCAGGGCGGGGGCGACCTC------------------------TCGCAGCTCACCATGGATGATGATATCGCCG`	`24 bp deleted → no frameshift`
81.9	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACA--------------------------CCATGGATGATGATATCGCCG`	`26 bp deleted → frameshift`
80.8	`AAGTGGCCAGGGCGGGGGCG--------------------------------CAGCTCACCATGGATGATGATATCGCCG`	`32 bp deleted → frameshift`
80.8	`AAGTGGCCAGG--------------------------------CTATTCTCGCAGCTCACCATGGATGATGATATCGCCG`	`32 bp deleted → frameshift`
77.4	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGC-----------------------------------------CG`	`41 bp deleted → frameshift`
75.0	`AAGTGGCCAGGGCGGGGGCGACC--------------------------------------ATGGATGATGATATCGCCG`	`38 bp deleted → frameshift`
73.2	`AAGTGGCCAGGGCGGGGGC----------------------------------AGCTCACCATGGATGATGATATCGCCG`	`34 bp deleted → frameshift`
70.5	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACA-----------------------------TGGATGATGATATCGCCG`	`29 bp deleted → frameshift`
63.6	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCA-------------------------------TGGATGATGATATCGCCG`	`31 bp deleted → frameshift`
62.8	`AAGTGGCCAGGGCGGGGGC-------------------------------------TCACCATGGATGATGATATCGCCG`	`37 bp deleted → frameshift`
60.0	`AAGTGGCCAGGGCGGGGGCGACCTCGG--------------------------------------ATGATGATATCGCCG`	`38 bp deleted → frameshift`
60.0	`AAGTGGCCAGGGCG--------------------------------------CAGCTCACCATGGATGATGATATCGCCG`	`38 bp deleted → frameshift`
54.0	`AAGTGGCCAGGGC----------------------------------------AGCTCACCATGGATGATGATATCGCCG`	`40 bp deleted → frameshift`
52.5	`AAGTGGCCAG---------------------------------------------CTCACCATGGATGATGATATCGCCG`	`45 bp deleted → no frameshift`
48.8	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCG------------------------------------------`	`42 bp deleted → no frameshift`
46.4	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGC-------------------------------------------CG`	`43 bp deleted → frameshift`
46.4	`AAGTGGCCAGGGC-------------------------------------------TCACCATGGATGATGATATCGCCG`	`43 bp deleted → frameshift`
44.4	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCG--------------------------------------------`	`44 bp deleted → frameshift`
40.0	`AAGTGGC----------------------------------------------AGCTCACCATGGATGATGATATCGCCG`	`46 bp deleted → frameshift`
39.0	`AAGTGGCCAGGGCGGGGGCGACCTCG---------------------------------------------------CCG`	`51 bp deleted → no frameshift`
38.0	`AAGTGGCCAGGGCGGGGG-----------------------------------------------ATGATGATATCGCCG`	`47 bp deleted → frameshift`
36.4	`AAGTGGCCAGGGCGGGG------------------------------------------------ATGATGATATCGCCG`	`48 bp deleted → no frameshift`
35.5	`AAGTGGCCA-----------------------------------------------------TGGATGATGATATCGCCG`	`53 bp deleted → frameshift`
34.4	`AAGTGGCCAGGGCGGG-------------------------------------------------ATGATGATATCGCCG`	`49 bp deleted → frameshift`
34.4	`AAGTGGC-------------------------------------------------TCACCATGGATGATGATATCGCCG`	`49 bp deleted → frameshift`
32.8	`AAGTGGCCAGGGCGGGGGCGACCTCGGC--------------------------------------------------CG`	`50 bp deleted → frameshift`
32.8	`AAGTGGCCAGGGCGG--------------------------------------------------ATGATGATATCGCCG`	`50 bp deleted → frameshift`
31.5	`AAGTGGCCAGGGCGGGGGCGA---------------------------------------------TGATGATATCGCCG`	`45 bp deleted → no frameshift`
30.0	`AAGTGGCCAGGGCGGGGGCGACCTCGGCTC----------------------------------------------GCCG`	`46 bp deleted → frameshift`
28.4	`AAGTGGCCAGGG-----------------------------------------------------ATGATGATATCGCCG`	`53 bp deleted → frameshift`
27.3	`AAGTGGCCAGGGCGGGGGCGA------------------------------------------------TGATATCGCCG`	`48 bp deleted → no frameshift`
26.8	`AAGTGGCCAGGGCGGGGGCGACCTCG------------------------------------------------------`	`54 bp deleted → no frameshift`
26.8	`AAGTGGCCAGG------------------------------------------------------ATGATGATATCGCCG`	`54 bp deleted → no frameshift`
26.0	`AAGTGG-----------------------------------------------------------ATGATGATATCGCCG`	`59 bp deleted → frameshift`
24.6	`AAGTGGCCA--------------------------------------------------CCATGGATGATGATATCGCCG`	`50 bp deleted → frameshift`
24.4	`AAGTGGCCAGGGCGGGGGCGACC--------------------------------------------------------G`	`56 bp deleted → frameshift`
23.4	`AAGTGGCCAGGGCGGGGGCGA---------------------------------------------------TATCGCCG`	`51 bp deleted → no frameshift`
23.2	`AAGTGGCCAGGGCGGGGGCG---------------------------------------------------------CCG`	`57 bp deleted → no frameshift`
22.2	`AAG----------------------------------------------------CTCACCATGGATGATGATATCGCCG`	`52 bp deleted → frameshift`
20.8	`AAGTGGCCAGGGCGGGGGC-----------------------------------------------------------CG`	`59 bp deleted → frameshift`
20.0	`AAGTGGCCAGGGCGGGGGCG------------------------------------------------------------`	`60 bp deleted → no frameshift`
17.4	`AAGTGGCC-----------------------------------------------------------------------G`	`71 bp deleted → frameshift`
17.2	`AAGTGGCCAGGGCG---------------------------------------------------------------CCG`	`63 bp deleted → no frameshift`
15.6	`AAGTGGCCAGGGC-----------------------------------------------------------------CG`	`65 bp deleted → frameshift`
14.8	`AAGTGGCCAGGGCG------------------------------------------------------------------`	`66 bp deleted → no frameshift`
12.9	`AAGTG---------------------------------------------------------------ATGATATCGCCG`	`63 bp deleted → no frameshift`
11.1	`AAGTG------------------------------------------------------------------ATATCGCCG`	`66 bp deleted → no frameshift`

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