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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529675_F | TCGTCGGCAGCGTCCCATGGTGAGCTGCGAGAAT | 60.4 | 1.00 |
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529675_R | GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT | 60.6 | 1.00 |
| mm3__chr19 46.49 Mbp_chr19_46493427_F | Primer3: not found at this Tm | N.d. | 0.60 |
| mm3__chr19 46.49 Mbp_chr19_46493427_R | Primer3: not found at this Tm | N.d. | 0.60 |
| mm4__chr2 82.30 Mbp_chr2_82297926_F | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4__chr2 82.30 Mbp_chr2_82297926_R | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4__chr17 80.15 Mbp_chr17_80152145_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4__chr17 80.15 Mbp_chr17_80152145_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4__chr4 189.19 Mbp_chr4_189193700_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr4 189.19 Mbp_chr4_189193700_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr19 19.26 Mbp_chr19_19261516_F | TCGTCGGCAGCGTCCTGTGAGCAGCAGGACGC | 61.1 | 0.25 |
| mm4__chr19 19.26 Mbp_chr19_19261516_R | GTCTCGTGGGCTCGGAGGCCTCCGAAAAGCCAAG | 60.3 | 0.25 |
| mm4__chr7 74.29 Mbp_chr7_74289644_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr7 74.29 Mbp_chr7_74289644_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr3 45.73 Mbp_chr3_45730581_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr3 45.73 Mbp_chr3_45730581_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chrX 3.63 Mbp_chrX_3633695_F | TCGTCGGCAGCGTCTAAAGTGGATGGTGCAGGCA | 59.5 | 0.20 |
| mm4__chrX 3.63 Mbp_chrX_3633695_R | GTCTCGTGGGCTCGGTCCTCAGAACTGGAGCAGGT | 60.1 | 0.20 |
| mm4__chr19 272.57 Kbp_chr19_272566_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr19 272.57 Kbp_chr19_272566_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr9 96.46 Mbp_chr9_96463015_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr9 96.46 Mbp_chr9_96463015_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr2 106.87 Mbp_chr2_106872673_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr2 106.87 Mbp_chr2_106872673_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3__chr1 20.55 Mbp_chr1_20553950_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3__chr1 20.55 Mbp_chr1_20553950_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr1 178.62 Mbp_chr1_178615136_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr1 178.62 Mbp_chr1_178615136_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3__chr12 131.56 Mbp_chr12_131560517_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3__chr12 131.56 Mbp_chr12_131560517_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3__chr12 131.07 Mbp_chr12_131070644_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3__chr12 131.07 Mbp_chr12_131070644_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr2 69.21 Mbp_chr2_69211892_F | TCGTCGGCAGCGTCGCTATTTCAGCCTACTCACCA | 57.4 | 0.13 |
| mm4__chr2 69.21 Mbp_chr2_69211892_R | GTCTCGTGGGCTCGGACGAGAGGAGTGGGTTGACT | 60.1 | 0.13 |
| mm4__chr17 1.58 Mbp_chr17_1580820_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr17 1.58 Mbp_chr17_1580820_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chrX 71.43 Mbp_chrX_71430314_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chrX 71.43 Mbp_chrX_71430314_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chrX 19.38 Mbp_chrX_19382174_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chrX 19.38 Mbp_chrX_19382174_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr9 133.20 Mbp_chr9_133196810_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr9 133.20 Mbp_chr9_133196810_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr8 119.78 Mbp_chr8_119784429_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr8 119.78 Mbp_chr8_119784429_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr3 8.80 Mbp_chr3_8799435_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr3 8.80 Mbp_chr3_8799435_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr14 32.75 Mbp_chr14_32750806_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr14 32.75 Mbp_chr14_32750806_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr1 66.86 Mbp_chr1_66858463_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr1 66.86 Mbp_chr1_66858463_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr16 2.22 Mbp_chr16_2223412_F | TCGTCGGCAGCGTCGGCAATCTGCTCCACCGTAA | 60.3 | 0.12 |
| mm4__chr16 2.22 Mbp_chr16_2223412_R | GTCTCGTGGGCTCGGTAGCGGTGTGGCCTTAAAGG | 60.0 | 0.12 |
| mm4__chr19 33.62 Mbp_chr19_33622703_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr19 33.62 Mbp_chr19_33622703_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr16 16.56 Mbp_chr16_16559438_F | TCGTCGGCAGCGTCCTTTAGTGGCCACCAGGAGG | 60.0 | 0.09 |
| mm4__chr16 16.56 Mbp_chr16_16559438_R | GTCTCGTGGGCTCGGCTTCAGTGACAGCAGAGGGC | 60.6 | 0.09 |
| mm4__chr11 68.21 Mbp_chr11_68213418_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr11 68.21 Mbp_chr11_68213418_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr16 67.88 Mbp_chr16_67881511_F | TCGTCGGCAGCGTCAGAGGGTGGTCTCTAGGCTG | 60.0 | 0.09 |
| mm4__chr16 67.88 Mbp_chr16_67881511_R | GTCTCGTGGGCTCGGCCCAGGGTGAAGTTCCACAA | 59.8 | 0.09 |
| mm4__chr18 5.30 Mbp_chr18_5295092_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr18 5.30 Mbp_chr18_5295092_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr16 78.52 Mbp_chr16_78516172_F | TCGTCGGCAGCGTCCAGAAGTACCTGCTGCCCAA | 59.9 | 0.08 |
| mm4__chr16 78.52 Mbp_chr16_78516172_R | GTCTCGTGGGCTCGGAGCAAACCCTCCCTGCATTT | 60.1 | 0.08 |
| mm4__chr6 41.33 Mbp_chr6_41334857_F | TCGTCGGCAGCGTCATGGAGTGGAGAGCAGGAGT | 59.9 | 0.08 |
| mm4__chr6 41.33 Mbp_chr6_41334857_R | GTCTCGTGGGCTCGGCTGGACTGGCGATCTGCTG | 60.5 | 0.08 |
| mm4__chr22 50.68 Mbp_chr22_50679182_F | TCGTCGGCAGCGTCGGGATCACCGACGAGAATGG | 60.2 | 0.08 |
| mm4__chr22 50.68 Mbp_chr22_50679182_R | GTCTCGTGGGCTCGGCCCTACAGACAGAGCCAGGA | 60.3 | 0.08 |
| mm4__chr1 51.33 Mbp_chr1_51330451_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr1 51.33 Mbp_chr1_51330451_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr21 36.70 Mbp_chr21_36700863_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr21 36.70 Mbp_chr21_36700863_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr18 73.64 Mbp_chr18_73639944_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr18 73.64 Mbp_chr18_73639944_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr9 23.82 Mbp_chr9_23820992_F | TCGTCGGCAGCGTCTCATCATTGCTCCTGCCCTG | 60.1 | 0.05 |
| mm4__chr9 23.82 Mbp_chr9_23820992_R | GTCTCGTGGGCTCGGCGGGGCGTTTGGAGACTTAA | 60.3 | 0.05 |
| mm3__chr14_GL000225v1_random 135.99 Kbp_chr14_GL000225v1_random_135990_F | TCGTCGGCAGCGTCAGGAATGGTTGTGCCTGCTG | 60.8 | 0.05 |
| mm3__chr14_GL000225v1_random 135.99 Kbp_chr14_GL000225v1_random_135990_R | GTCTCGTGGGCTCGGAGCAGGGGCAGATGCAAG | 60.0 | 0.05 |
| mm4__chr3 98.90 Mbp_chr3_98901438_F | TCGTCGGCAGCGTCGCGGGAGAACAAGAGGCAG | 60.7 | 0.05 |
| mm4__chr3 98.90 Mbp_chr3_98901438_R | GTCTCGTGGGCTCGGGGGACGAAGGAGGAGTAGGT | 60.0 | 0.05 |
| mm4__chr17 8.12 Mbp_chr17_8121693_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr17 8.12 Mbp_chr17_8121693_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr20 41.33 Mbp_chr20_41325975_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr20 41.33 Mbp_chr20_41325975_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr2 34.12 Mbp_chr2_34116602_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr2 34.12 Mbp_chr2_34116602_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr11 79.33 Mbp_chr11_79325700_F | TCGTCGGCAGCGTCAGGAAAGTACCCAGTACCCAGT | 60.1 | 0.03 |
| mm4__chr11 79.33 Mbp_chr11_79325700_R | GTCTCGTGGGCTCGGTTGGGTGTGTTCTCGTGGAG | 59.8 | 0.03 |
| mm4__chr7 66.24 Mbp_chr7_66244012_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr7 66.24 Mbp_chr7_66244012_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr12 85.67 Mbp_chr12_85668080_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr12 85.67 Mbp_chr12_85668080_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chrX 11.70 Mbp_chrX_11702545_F | TCGTCGGCAGCGTCTGCAAGCTCACAGTTAGGAGG | 59.9 | 0.03 |
| mm4__chrX 11.70 Mbp_chrX_11702545_R | GTCTCGTGGGCTCGGGGAAAGCTCTGAGGTGACCC | 60.0 | 0.03 |
| mm4__chr19 3.20 Mbp_chr19_3201221_F | TCGTCGGCAGCGTCCCTGGGCTCCAAATTCTGCA | 60.6 | 0.02 |
| mm4__chr19 3.20 Mbp_chr19_3201221_R | GTCTCGTGGGCTCGGTCGCTCCCCTCCTGTATTCA | 60.0 | 0.02 |
| mm4__chr1 115.64 Mbp_chr1_115642029_F | TCGTCGGCAGCGTCTCTGCCTCTCCAGGAGCC | 60.3 | 0.02 |
| mm4__chr1 115.64 Mbp_chr1_115642029_R | GTCTCGTGGGCTCGGCCTCTCGCGGGACTCACC | 61.8 | 0.02 |
| mm4__chrY 5.90 Mbp_chrY_5901812_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chrY 5.90 Mbp_chrY_5901812_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chrX 92.79 Mbp_chrX_92785178_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chrX 92.79 Mbp_chrX_92785178_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr6 1.61 Mbp_chr6_1608153_F | TCGTCGGCAGCGTCCCGCAAACCACCTGGAAAAG | 59.9 | 0.02 |
| mm4__chr6 1.61 Mbp_chr6_1608153_R | GTCTCGTGGGCTCGGGAGGTTGCTCTGAATCCCGG | 60.4 | 0.02 |
| mm4__chr12 6.28 Mbp_chr12_6275772_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr12 6.28 Mbp_chr12_6275772_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr20 34.98 Mbp_chr20_34978655_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr20 34.98 Mbp_chr20_34978655_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr2 56.89 Mbp_chr2_56886058_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr2 56.89 Mbp_chr2_56886058_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3__chr3 54.66 Mbp_chr3_54663358_F | TCGTCGGCAGCGTCGGCACCTCCTGTTGTCTTCA | 59.8 | 0.01 |
| mm3__chr3 54.66 Mbp_chr3_54663358_R | GTCTCGTGGGCTCGGAAGTCTTGGTGCCTGGAAGG | 59.8 | 0.01 |
| mm4__chr15 63.34 Mbp_chr15_63338664_F | TCGTCGGCAGCGTCTGCAAAGACTCACTGAAGGGG | 60.2 | 0.01 |
| mm4__chr15 63.34 Mbp_chr15_63338664_R | GTCTCGTGGGCTCGGAATGCAGCTCTGGCACTGAT | 60.0 | 0.01 |
| mm4__chr9 95.07 Mbp_chr9_95067561_F | TCGTCGGCAGCGTCGAACCAGCCAGCGCAAAG | 59.7 | 0.01 |
| mm4__chr9 95.07 Mbp_chr9_95067561_R | GTCTCGTGGGCTCGGACCCCTCACCCTAAGAACCA | 59.8 | 0.01 |
| mm4__chr16 4.39 Mbp_chr16_4392710_F | TCGTCGGCAGCGTCCCACTAGGCAGGAAGGAGGA | 60.3 | 0.01 |
| mm4__chr16 4.39 Mbp_chr16_4392710_R | GTCTCGTGGGCTCGGGCCCACACCTGGCTTCTATT | 60.0 | 0.01 |
| mm4__chr2 214.72 Mbp_chr2_214717606_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr2 214.72 Mbp_chr2_214717606_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr2 241.54 Mbp_chr2_241541849_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr2 241.54 Mbp_chr2_241541849_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr8 10.52 Mbp_chr8_10517863_F | TCGTCGGCAGCGTCAGGTAAGAGGTGGCATCCCT | 59.9 | 0.00 |
| mm4__chr8 10.52 Mbp_chr8_10517863_R | GTCTCGTGGGCTCGGACATGCCCAGGACAATCCAG | 60.0 | 0.00 |
| mm4__chr19 8.15 Mbp_chr19_8146124_F | TCGTCGGCAGCGTCCTTCTTCTCCCTCCCGCAAG | 60.1 | 0.00 |
| mm4__chr19 8.15 Mbp_chr19_8146124_R | GTCTCGTGGGCTCGGGTGAAGGCTTCTGCTCCCAG | 60.6 | 0.00 |
| mm4__chr1 25.03 Mbp_chr1_25034735_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 25.03 Mbp_chr1_25034735_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr17 13.79 Mbp_chr17_13785229_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr17 13.79 Mbp_chr17_13785229_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 2.24 Mbp_chr1_2238786_F | TCGTCGGCAGCGTCTGCTGAAGGTGTACACGTCC | 59.9 | 0.00 |
| mm4__chr1 2.24 Mbp_chr1_2238786_R | GTCTCGTGGGCTCGGGGAATACCTCCCTCCCCAGT | 60.0 | 0.00 |
| mm4__chr7 157.84 Mbp_chr7_157835613_F | TCGTCGGCAGCGTCAGGAAACAGGGATCTGCAGC | 60.0 | 0.00 |
| mm4__chr7 157.84 Mbp_chr7_157835613_R | GTCTCGTGGGCTCGGTTTCCCACGCATTTGCAGTG | 59.9 | 0.00 |
| mm4__chrX 107.74 Mbp_chrX_107737157_F | TCGTCGGCAGCGTCACACAGGAATCCAGCAAGGG | 59.9 | 0.00 |
| mm4__chrX 107.74 Mbp_chrX_107737157_R | GTCTCGTGGGCTCGGGGGACTTGGCCAGGATCTTC | 60.1 | 0.00 |
| mm4__chr9 124.00 Mbp_chr9_123999896_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr9 124.00 Mbp_chr9_123999896_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr10 132.96 Mbp_chr10_132964793_F | TCGTCGGCAGCGTCTTTAACAGGCTCATCGGCGT | 60.0 | 0.00 |
| mm4__chr10 132.96 Mbp_chr10_132964793_R | GTCTCGTGGGCTCGGCAGTGGGAGGAGGGAGGTG | 60.9 | 0.00 |
| mm4__chr15 37.05 Mbp_chr15_37049734_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr15 37.05 Mbp_chr15_37049734_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr16 1.17 Mbp_chr16_1165722_F | TCGTCGGCAGCGTCCCTGAGGATGTAGAGGGGCT | 60.1 | 0.00 |
| mm4__chr16 1.17 Mbp_chr16_1165722_R | GTCTCGTGGGCTCGGGTTCATCTGGTGGGGAACGT | 59.9 | 0.00 |
| mm4__chr9 93.59 Mbp_chr9_93592819_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr9 93.59 Mbp_chr9_93592819_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr8 134.14 Mbp_chr8_134144310_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr8 134.14 Mbp_chr8_134144310_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 169.93 Mbp_chr5_169929056_F | TCGTCGGCAGCGTCTTGTGCCCTGAGAAGAGCAG | 59.9 | 0.00 |
| mm4__chr5 169.93 Mbp_chr5_169929056_R | GTCTCGTGGGCTCGGGGCACTCTGGTTCCCTTTCA | 59.8 | 0.00 |
| mm4__chr22 28.97 Mbp_chr22_28965484_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr22 28.97 Mbp_chr22_28965484_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr2 38.10 Mbp_chr2_38098428_F | TCGTCGGCAGCGTCTTGAGGGAGAAGCGTTCCTG | 59.6 | 0.00 |
| mm4__chr2 38.10 Mbp_chr2_38098428_R | GTCTCGTGGGCTCGGCCCTCCCCACACTTCCTTTT | 59.5 | 0.00 |
| mm4__chr18 75.87 Mbp_chr18_75870883_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr18 75.87 Mbp_chr18_75870883_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr11 119.47 Mbp_chr11_119470751_F | TCGTCGGCAGCGTCCGGCCCATTTGTAGAGCTCA | 60.1 | 0.00 |
| mm4__chr11 119.47 Mbp_chr11_119470751_R | GTCTCGTGGGCTCGGGCAGGCATCAGAGAGTCTGG | 60.1 | 0.00 |
| mm4__chr10 118.50 Mbp_chr10_118501213_F | TCGTCGGCAGCGTCCCTTCCTGCCTCATCTGCTC | 60.1 | 0.00 |
| mm4__chr10 118.50 Mbp_chr10_118501213_R | GTCTCGTGGGCTCGGACTACACTTCCAGGAGGGCT | 59.8 | 0.00 |
| mm3__chr15 67.82 Mbp_chr15_67823618_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr15 67.82 Mbp_chr15_67823618_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr1 13.50 Mbp_chr1_13497184_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr1 13.50 Mbp_chr1_13497184_R | Primer3: not found at this Tm | N.d. | 0.00 |
Primers underlined, off-targets in bold.
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529675 |
CCATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTC CTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG |
| mm4__chr19 19.26 Mbp_chr19_19261516 |
CTGTGAGCAGCAGGACGCCCCAGGCCGCGGCCCAGAGCGCGCCGGGACCGAGGGCCGCCCGAGCGCACACCTGGGGGGCG GGCACGGGGCGCTCAGTCCAGCCTCCCAGCCTTGGCTTTTCGGAGGCCT |
| mm4__chrX 3.63 Mbp_chrX_3633695 |
TAAAGTGGATGGTGCAGGCAGAGATACCCTGGGCGCTGTGAGCTGATGTTCTCCCTCCTACTCAGCTGGAAAAACACCCT CCTAAACTATCAGCTGACACACTACCTGCTCCAGTTCTGAGGA |
| mm4__chr2 69.21 Mbp_chr2_69211892 |
GCTATTTCAGCCTACTCACCATATGTATTCCCACCTGTGGACCTCTGCTCACACCACCCCCGGCCCAAATTATGCTGTTC CTTCTCTTCTCATTGTAAGTCAACCCACTCCTCTCGT |
| mm4__chr16 2.22 Mbp_chr16_2223412 |
GGCAATCTGCTCCACCGTAACCACGCCCTCCCCACAGTCCCGCCCCATCTTCGCTCTCAGCGCGCCCGGCCCCGCCTCTG CCCTTTAAGGCCACACCGCTA |
| mm4__chr16 16.56 Mbp_chr16_16559438 |
CTTTAGTGGCCACCAGGAGGTGAATGACTCATGCTACCTTTTCTCAGCAAAGGCTCTTCAGGATGGGACACTCCAAGTTT TTAACTCCCAGCAACAGTTCGGCTCACAGCGAGACCGGGCCCTCTGCTGTCACTGAAG |
| mm4__chr16 67.88 Mbp_chr16_67881511 |
AGAGGGTGGTCTCTAGGCTGCCTCACATAGCCTGAGGTGCTTCTCGCCTATGGCAGGGATGCAGCCATGGGATCCCGGCT CACAGAGCACCAGGTAAGTGAATGAGCCCACTTTGTACTTGTGGAACTTCACCCTGGG |
| mm4__chr16 78.52 Mbp_chr16_78516172 |
CAGAAGTACCTGCTGCCCAAGACCACAGCTGACAGCGCCCCGGGAGTCCTTCACACTCTTCTCTGAGCTCTTCCATCCTT AAACAGACATCTATGCCAAATGCAGGGAGGGTTTGCT |
| mm4__chr6 41.33 Mbp_chr6_41334857 |
ATGGAGTGGAGAGCAGGAGTCCAGGGGCAGGCAAAGCAGTGGACTTGGAATCGAGTCCACTTGACAAGTTCCAATTCCCG TCTCAGTTATTACCTCTGTTCACAGAGCTCCCGGCAGCAGATCGCCAGTCCAG |
| mm4__chr22 50.68 Mbp_chr22_50679182 |
GGGATCACCGACGAGAATGGCTGGCAGGAGATCCACCAGGTGTGCAGGGAGCCGAGGTGGGGTCCCGGCCTCTGTGTGCT GGGTTGGGGGTCCTGGCTCTGTCTGTAGGG |
| mm4__chr9 23.82 Mbp_chr9_23820992 |
TCATCATTGCTCCTGCCCTGGGCGCCCGCGGCCCTACACCACCTCCGCACACAACGCGGCCGGTGTTAAGTCTCCAAACG CCCCG |
| mm3__chr14_GL000225v1_random 135.99 Kbp_chr14_GL000225v1_random_135990 |
AGGAATGGTTGTGCCTGCTGCAGAAACTCCGGGCTCGCCAGGAGCTCATCCAGCAGCTGGCCGGAGGGGAGTGCAGAAGA GCACCCCGGCTCCCAGAGCGCCTGGGAGAGCGCCGGGATGCCTTGCATCTGCCCCTGCT |
| mm4__chr3 98.90 Mbp_chr3_98901438 |
GCGGGAGAACAAGAGGCAGCCCTCGCCTCACCCCGCGCCGGGACCCTTCCGCCCCTCACCCCGCTTTCACCTACTCCTCC TTCGTCCC |
| mm4__chr11 79.33 Mbp_chr11_79325700 |
AGGAAAGTACCCAGTACCCAGTACTCTACCCAGGCGCTCAGTGAACAGAGGTTATTGTGATCATTTTATAATTCACTGGG TCAAAGATACAGATGATTCACCAGATCTCTATCATGTCTCCACGAGAACACACCCAA |
| mm4__chrX 11.70 Mbp_chrX_11702545 |
TGCAAGCTCACAGTTAGGAGGATGCAAATCTAGTTGAGCCAAAGAGTCCCTGGAGCTCTGGGAGCCTAGGTGGAGGGTCA CCTCAGAGCTTTCC |
| mm4__chr19 3.20 Mbp_chr19_3201221 |
CCTGGGCTCCAAATTCTGCATGTAGCTTCTCCCGGGCGCGGGCCGAGCCGAGGTGGATGAGGAGAGTCCTGGGCTTGGCG GGGCCCCGCAGACCCCAGGCCATCAGTCGGGGATTGAATACAGGAGGGGAGCGA |
| mm4__chr1 115.64 Mbp_chr1_115642029 |
TCTGCCTCTCCAGGAGCCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNGGTGAGTCCCGCGAGAGG |
| mm4__chr6 1.61 Mbp_chr6_1608153 |
CCGCAAACCACCTGGAAAAGGAGTCTCCAGAGACCCCGGCGCACAGCAGGCCAGGAGGCAGCTGCCCCGGGATTCAGAGC AACCTC |
| mm3__chr3 54.66 Mbp_chr3_54663358 |
GGCACCTCCTGTTGTCTTCAGCCCTTCTGGACTCCAGGGGCAGTGTGAGCCGAGGTGTCCACCCCTCATGCCTTCATGGC CCTGGGCCTCTCAGTTATGTATGTGATGACTTGGCCTTCCAGGCACCAAGACTT |
| mm4__chr15 63.34 Mbp_chr15_63338664 |
TGCAAAGACTCACTGAAGGGGGGACCCCAGTGAGCCCCACACCTAGTCTCACAAGGCGCCTGGTTCCCGTGGCAGCCAGG GAGCTCTCAGCCAACTTCTTGAGGGCATCAGTGCCAGAGCTGCATT |
| mm4__chr9 95.07 Mbp_chr9_95067561 |
GAACCAGCCAGCGCAAAGCTGACTTCATGGCCTCGGCTCTCAGGGCACCAGGTAGTTTTTCTCGAGAGTAGACTGTGTAG AATAGTACCCTTCGCTGAAACAGCCTTTCTCAACTTGGTTCTTAGGGTGAGGGGT |
| mm4__chr16 4.39 Mbp_chr16_4392710 |
CCACTAGGCAGGAAGGAGGATTGCCAGAGAAGGAGCTCCCAGAAGGCCTCTCCCTCGAGCCGGCCCCTCGGCTCACACCG GCCCTGGCTGCCAGCAGCGGTTTTCCTAATAGAAGCCAGGTGTGGGC |
| mm4__chr8 10.52 Mbp_chr8_10517863 | AGGTAAGAGGTGGCATCCCTTCCCCCAACCTCTGCTCCTAGCGGGCCTGGCACAGACCTGGATTGTCCTGGGCATGT |
| mm4__chr19 8.15 Mbp_chr19_8146124 |
CTTCTTCTCCCTCCCGCAAGAGGCCGCTTCCCGCTCACCTCGGCTCACCCCGCAGCTGGGAGCCAGCGTCCCATCCGCAC AGGTGCACAGGTTGGGCTGGGAGCAGAAGCCTTCAC |
| mm4__chr1 2.24 Mbp_chr1_2238786 |
TGCTGAAGGTGTACACGTCCACTGAGGCCACCTGCGCCGCTGCCTGTGTCCTGTGGGCTGGCACTCACCGAGTGAGAGCT GTGTGCAGAACCTCCGCTGTCAGCGGGCCAGGCCACACTGGGGAGGGAGGTATTCC |
| mm4__chr7 157.84 Mbp_chr7_157835613 |
AGGAAACAGGGATCTGCAGCAATGCCTCGGCTCTCAGGGCGCTCGGGAGGGTGCAGGGGCGAAATTAACAACATAGCCGA AGGGTGCACTGCAAATGCGTGGGAAA |
| mm4__chrX 107.74 Mbp_chrX_107737157 |
ACACAGGAATCCAGCAAGGGAAGTTGCAAAGGCCAGACCACGGCTCCCAGGGCCCCTGGACCAATGGGGGACAAGGGGCA TGCTGAGGTATAAATGACTTGTGAAGATCCTGGCCAAGTCCC |
| mm4__chr10 132.96 Mbp_chr10_132964793 |
TTTAACAGGCTCATCGGCGTGGCCCTAGGCCCTGCCCTCTGTGAGCCCAGGTCACCCCCAGGACGCCCAGCGGCTGCTGC GCCACCTCCCTCCTCCCACTG |
| mm4__chr16 1.17 Mbp_chr16_1165722 |
CCTGAGGATGTAGAGGGGCTGCCTCCCTGCCCCCACCCCGGCTCACAGTGCGGGTGGCTCCCGGCTCTCCTGAGTTGTGC TGGCCACCTCACGTTCCCCACCAGATGAAC |
| mm4__chr5 169.93 Mbp_chr5_169929056 |
TTGTGCCCTGAGAAGAGCAGATGGAAAGCTTGAGGTCAACCTGGGCCCACAGCTGGCCAGGTAGACTGAAGTTCTGTCAT GCCCTGCCTTGAAAGGGAACCAGAGTGCC |
| mm4__chr2 38.10 Mbp_chr2_38098428 |
TTGAGGGAGAAGCGTTCCTGTAATGGAGGGGAAATGAATAAAACCTCGACTCCCAGCTCGACAGGGACCTTTCTTCTCCT AGCCAGCTGGAATGCAAGCTTTAAAAAAAAAGGAAGTGTGGGGAGGG |
| mm4__chr11 119.47 Mbp_chr11_119470751 | CGGCCCATTTGTAGAGCTCAGAGACCCCGGCTCCCACCCCGCCTGGCCCCTCTCCCTCCAGACTCTCTGATGCCTGC |
| mm4__chr10 118.50 Mbp_chr10_118501213 |
CCTTCCTGCCTCATCTGCTCTGCACATCCAGACCTTGGGTCACAGCCCTCCTGGAAGTGTGGTCACAGCCCTCCTGGAAG TGTAGT |