CRISPOR

Micro-homology scoring of potential deletions

Guide: AGCCGAGGTCGCCCCCGCCC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	(Wild-type sequence)
444.6	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCC------ACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`6 bp deleted → no frameshift`
329.4	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCC------------ACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`12 bp deleted → no frameshift`
311.6	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCC-----ACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`5 bp deleted → frameshift`
294.4	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCC--------------------GAGTCCTTAGGCCGCCAGGGG`	`20 bp deleted → frameshift`
283.2	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCG---------------GCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`15 bp deleted → no frameshift`
255.2	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCC---------ACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`9 bp deleted → no frameshift`
244.2	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGC------------------GCCGAGTCCTTAGGCCGCCAGGGG`	`18 bp deleted → no frameshift`
242.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCC----------ACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`10 bp deleted → frameshift`
230.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCC-----------GGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`11 bp deleted → frameshift`
230.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCC-----------ACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`11 bp deleted → frameshift`
219.6	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCC------------GGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`12 bp deleted → no frameshift`
218.4	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCC--------------------------GAGTCCTTAGGCCGCCAGGGG`	`26 bp deleted → frameshift`
210.0	`GAATAGCCGGGCGCGCTGTGAGCC---------------------ACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`21 bp deleted → no frameshift`
191.4	`GAATAGCCGGGCGCGCTGTGAGCCGAG-----------------------------------TCCTTAGGCCGCCAGGGG`	`35 bp deleted → frameshift`
190.2	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCG-----------------------AGTCCTTAGGCCGCCAGGGG`	`23 bp deleted → frameshift`
188.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGG---------------CCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`15 bp deleted → no frameshift`
180.6	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGC------------------------GCCGAGTCCTTAGGCCGCCAGGGG`	`24 bp deleted → no frameshift`
179.6	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGC----------------GCGCCGAGTCCTTAGGCCGCCAGGGG`	`16 bp deleted → frameshift`
179.6	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCC----------------GGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`16 bp deleted → frameshift`
170.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCC-----------------GGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`17 bp deleted → frameshift`
170.8	`GAATAGCCGGGCGCGC------------------------------------------CGAGTCCTTAGGCCGCCAGGGG`	`42 bp deleted → no frameshift`
165.0	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCC------------------------------------AGGGG`	`36 bp deleted → no frameshift`
162.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCC------------------GGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`18 bp deleted → no frameshift`
155.4	`GAATAGCCGGGCGCGCTGTGAGCCG---------------------------GCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`27 bp deleted → no frameshift`
154.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCC-------------------GAGTCCTTAGGCCGCCAGGGG`	`19 bp deleted → frameshift`
140.0	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCG---------------------GCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`21 bp deleted → no frameshift`
133.2	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGC----------------------GCGCCGAGTCCTTAGGCCGCCAGGGG`	`22 bp deleted → frameshift`
121.2	`GAATAGCCGGGC--------------------------------CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`32 bp deleted → frameshift`
120.4	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCC------------------------GAGTCCTTAGGCCGCCAGGGG`	`24 bp deleted → no frameshift`
115.2	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCC---------------------------------GCCAGGGG`	`33 bp deleted → no frameshift`
114.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCC-------------------------TTAGGCCGCCAGGGG`	`25 bp deleted → frameshift`
114.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCC-------------------------GAGTCCTTAGGCCGCCAGGGG`	`25 bp deleted → frameshift`
114.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGG-------------------------CGCGCCGAGTCCTTAGGCCGCCAGGGG`	`25 bp deleted → frameshift`
110.4	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTC--------------------CGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`20 bp deleted → frameshift`
109.2	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCC--------------------------TTAGGCCGCCAGGGG`	`26 bp deleted → frameshift`
108.0	`GAATAGCCGGGCGCGC----------------------------------------GCCGAGTCCTTAGGCCGCCAGGGG`	`40 bp deleted → frameshift`
99.9	`GAATAGCCGGGCGCGCTGTG----------------------GCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`22 bp deleted → frameshift`
98.8	`GAATAGCCGGGCGCGC----------------------------CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`28 bp deleted → frameshift`
97.6	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCC------------------------------------------AGGGG`	`42 bp deleted → no frameshift`
94.2	`GAATAGCC-------------------------------------ACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`37 bp deleted → frameshift`
94.0	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCC-----------------------------TTAGGCCGCCAGGGG`	`29 bp deleted → frameshift`
94.0	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCG-----------------------------AGTCCTTAGGCCGCCAGGGG`	`29 bp deleted → frameshift`
92.8	`GAATAGCCGG-------------------------------------------CGCGCCGAGTCCTTAGGCCGCCAGGGG`	`43 bp deleted → frameshift`
91.5	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTC----------------------------------CTTAGGCCGCCAGGGG`	`34 bp deleted → frameshift`
90.3	`GAATAGCCGGGCGCGCTG------------------------GCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`24 bp deleted → no frameshift`
89.2	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCC------------------------------TTAGGCCGCCAGGGG`	`30 bp deleted → no frameshift`
89.2	`GAATAGCCGGGCGCGCTGTGAGCCG------------------------------CGCCGAGTCCTTAGGCCGCCAGGGG`	`30 bp deleted → no frameshift`
89.2	`GAATAGCCGGGCGC------------------------------CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`30 bp deleted → no frameshift`
88.8	`GAATAGCCGGGCGC--------------------------------------------CGAGTCCTTAGGCCGCCAGGGG`	`44 bp deleted → frameshift`
85.2	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCC---------------------------------------GCCAGGGG`	`39 bp deleted → no frameshift`
84.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCC-------------------------------TTAGGCCGCCAGGGG`	`31 bp deleted → frameshift`
84.8	`GAATAGCCGGGCGCGCTGTGAGC-------------------------------GCGCCGAGTCCTTAGGCCGCCAGGGG`	`31 bp deleted → frameshift`
80.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCC--------------------------------GCCAGGGG`	`32 bp deleted → frameshift`
80.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCC--------------------------------TTAGGCCGCCAGGGG`	`32 bp deleted → frameshift`
80.8	`GAATAGCCGGGCGCGCTGTGAGCCG--------------------------------CCGAGTCCTTAGGCCGCCAGGGG`	`32 bp deleted → frameshift`
76.8	`GAATAGCCGGGCGCGCTGTGAGC---------------------------------GCCGAGTCCTTAGGCCGCCAGGGG`	`33 bp deleted → no frameshift`
76.8	`GAATAGCCGG---------------------------------CCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`33 bp deleted → no frameshift`
72.8	`GAATAGCCGGGCGCGCTGTGAGCCG------------------------------------------------CCAGGGG`	`48 bp deleted → no frameshift`
69.6	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCC-----------------------------------AGGGG`	`35 bp deleted → frameshift`
67.5	`GAATAGCCGGGCGCGCTGTGAG----------------------------------------TCCTTAGGCCGCCAGGGG`	`40 bp deleted → frameshift`
63.6	`GAATAGCCGGGCGCGCT-------------------------------TCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`31 bp deleted → frameshift`
62.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCC-------------------------------------GCCAGGGG`	`37 bp deleted → frameshift`
62.8	`GAATAGCCGGGCGCG-------------------------------------GCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`37 bp deleted → frameshift`
62.4	`GAATAGCCG---------------------------------------------------AGTCCTTAGGCCGCCAGGGG`	`51 bp deleted → no frameshift`
61.0	`GAATAGCCGGGCGCGCTGTGAGCCGAGG------------------------------------------CCGCCAGGGG`	`42 bp deleted → no frameshift`
60.0	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCC--------------------------------------GCCAGGGG`	`38 bp deleted → frameshift`
60.0	`GAATAGCCGGGCGCGC--------------------------------------GCGCCGAGTCCTTAGGCCGCCAGGGG`	`38 bp deleted → frameshift`
56.8	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCC---------------------------------------AGGGG`	`39 bp deleted → no frameshift`
56.8	`GAATAGCCGGGCG---------------------------------------GCGCGCCGAGTCCTTAGGCCGCCAGGGG`	`39 bp deleted → no frameshift`
54.0	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCC----------------------------------------AGGGG`	`40 bp deleted → frameshift`
51.6	`GAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCC-----------------------------------------AGGGG`	`41 bp deleted → frameshift`
51.6	`GAATAGCCGGGCGCGCTGTGAGCC-----------------------------------------TTAGGCCGCCAGGGG`	`41 bp deleted → frameshift`
46.8	`GAATAGCCGGGCGCGCTGTGAGCC---------------------------------------------------AGGGG`	`51 bp deleted → no frameshift`
42.0	`GAATAGCCGGGCGCG---------------------------------------------AGTCCTTAGGCCGCCAGGGG`	`45 bp deleted → no frameshift`
41.0	`GAATAGCCGGGCGCGCTGTGAGCCGAGG--------------------------------------------------GG`	`50 bp deleted → frameshift`
40.0	`GAATAGCCGGGC----------------------------------------------CGAGTCCTTAGGCCGCCAGGGG`	`46 bp deleted → frameshift`
40.0	`GAATAGCCG----------------------------------------------CGCCGAGTCCTTAGGCCGCCAGGGG`	`46 bp deleted → frameshift`
38.0	`GAATAGCCGGGCG-----------------------------------------------AGTCCTTAGGCCGCCAGGGG`	`47 bp deleted → frameshift`
38.0	`GAATAGC-----------------------------------------------GCGCCGAGTCCTTAGGCCGCCAGGGG`	`47 bp deleted → frameshift`
36.4	`GAATAGCCG------------------------------------------------CCGAGTCCTTAGGCCGCCAGGGG`	`48 bp deleted → no frameshift`
34.4	`GAATAGC-------------------------------------------------GCCGAGTCCTTAGGCCGCCAGGGG`	`49 bp deleted → frameshift`
33.3	`GAATAGCCGGGCGCGCTGT--------------------------------------------CCTTAGGCCGCCAGGGG`	`44 bp deleted → frameshift`
33.0	`GAATAGCCGGGCGCGC----------------------------------------------------------CAGGGG`	`58 bp deleted → frameshift`
32.8	`GAATAGCCG----------------------------------------------------------------CCAGGGG`	`64 bp deleted → frameshift`
31.2	`GAATAGCCGGGC-----------------------------------------------------------CGCCAGGGG`	`59 bp deleted → frameshift`
31.2	`GAATAGCCGGGCGCGCTGTGAGCCGAGG---------------------------------------------------G`	`51 bp deleted → no frameshift`
30.0	`GAATAGCCGGGCGC------------------------------------------------------------CAGGGG`	`60 bp deleted → no frameshift`
29.6	`GAATAGCCGGGCGCGCTGTGAGCCGAGG----------------------------------------------------`	`52 bp deleted → frameshift`
28.5	`GAATAGCCGGGCGCGCTGTGAG-----------------------------------------------GCCGCCAGGGG`	`47 bp deleted → frameshift`
25.8	`GAATAGCCGGGCGCGCT-------------------------------------------------TAGGCCGCCAGGGG`	`49 bp deleted → frameshift`
25.6	`GAATAGCCGGGCGCGC-------------------------------------------------------CGCCAGGGG`	`55 bp deleted → frameshift`
23.2	`GAATAGCCGGGCGC---------------------------------------------------------CGCCAGGGG`	`57 bp deleted → no frameshift`
23.2	`GAATAGCC---------------------------------------------------------TTAGGCCGCCAGGGG`	`57 bp deleted → no frameshift`
21.0	`GAATAGCC-------------------------------------------------------------------AGGGG`	`67 bp deleted → frameshift`
20.0	`GAATAGCCGG------------------------------------------------------------CCGCCAGGGG`	`60 bp deleted → no frameshift`
19.8	`GAATAGCCGGG--------------------------------------------------------------------G`	`68 bp deleted → frameshift`
19.2	`GAATAGCCGGGCGCGCTGTGAG-------------------------------------------------------GGG`	`55 bp deleted → frameshift`
19.2	`GAATAGCCGGG---------------------------------------------------------------------`	`69 bp deleted → no frameshift`
18.3	`GAATAG--------------------------------------------------------TCCTTAGGCCGCCAGGGG`	`56 bp deleted → frameshift`
18.0	`GAATAGCCGGGC--------------------------------------------------------------CAGGGG`	`62 bp deleted → frameshift`
17.2	`GAATAG---------------------------------------------------------------GCCGCCAGGGG`	`63 bp deleted → no frameshift`
15.6	`GA-----------------------------------------------------------GTCCTTAGGCCGCCAGGGG`	`59 bp deleted → frameshift`
14.0	`GAATAGCCGGG-------------------------------------------------------------------GG`	`67 bp deleted → frameshift`
12.0	`GAATAGCCGG----------------------------------------------------------------------`	`70 bp deleted → frameshift`
8.7	`GAATAG-----------------------------------------------------------------------GGG`	`71 bp deleted → frameshift`

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