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Contents:

PCR primers for off-targets of GCCCCCGCCCTGGCCACTTC CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529699_F TCGTCGGCAGCGTCTGGTGAGCTGCGAGAATAGC 60.1 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529699_R GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT 60.6 1.00
mm4__chr7 10.38 Mbp_chr7_10379293_F TCGTCGGCAGCGTCGTCCAAGAAGTGATCATTGCCC 59.5 0.59
mm4__chr7 10.38 Mbp_chr7_10379293_R GTCTCGTGGGCTCGGACAGGAATGCATGTGGGGTT 59.8 0.59
mm4__chr16 75.22 Mbp_chr16_75219271_F TCGTCGGCAGCGTCCAGGAGAGGGTGCATTCTGG 60.1 0.56
mm4__chr16 75.22 Mbp_chr16_75219271_R GTCTCGTGGGCTCGGACACCTTACCCTGCACAAGG 59.8 0.56
mm4__chr9 135.61 Mbp_chr9_135612564_F TCGTCGGCAGCGTCGATGGAGGCCAGAAGCTGTC 60.4 0.56
mm4__chr9 135.61 Mbp_chr9_135612564_R GTCTCGTGGGCTCGGCATCCTGCATCCTGCCTACA 59.5 0.56
mm4__chr8 100.78 Mbp_chr8_100780078_F Primer3: not found at this Tm N.d. 0.54
mm4__chr8 100.78 Mbp_chr8_100780078_R Primer3: not found at this Tm N.d. 0.54
mm4__chr17 82.88 Mbp_chr17_82879002_F Primer3: not found at this Tm N.d. 0.52
mm4__chr17 82.88 Mbp_chr17_82879002_R Primer3: not found at this Tm N.d. 0.52
mm4__chr7 98.22 Mbp_chr7_98218122_F TCGTCGGCAGCGTCAAGCAGTGAGGGTCAAAGGG 59.8 0.52
mm4__chr7 98.22 Mbp_chr7_98218122_R GTCTCGTGGGCTCGGCCCCAGCAGCTTCCCAAA 59.8 0.52
mm4__chr2 25.29 Mbp_chr2_25285104_F Primer3: not found at this Tm N.d. 0.51
mm4__chr2 25.29 Mbp_chr2_25285104_R Primer3: not found at this Tm N.d. 0.51
mm3__chr4 148.50 Mbp_chr4_148504571_F TCGTCGGCAGCGTCAGGGCTACATGATGAGGGGA 59.7 0.51
mm3__chr4 148.50 Mbp_chr4_148504571_R GTCTCGTGGGCTCGGCAGACCACTGGCTTACCTCC 59.7 0.51
mm4__chr12 54.47 Mbp_chr12_54473591_F TCGTCGGCAGCGTCCACAGTCACCTTCCTCCCAC 59.9 0.46
mm4__chr12 54.47 Mbp_chr12_54473591_R GTCTCGTGGGCTCGGCTCAGCCAGAACCGAGACTC 59.8 0.46
mm3__chr7 47.12 Mbp_chr7_47122454_F TCGTCGGCAGCGTCAGGCAGGATTAACACAGGACC 59.7 0.40
mm3__chr7 47.12 Mbp_chr7_47122454_R GTCTCGTGGGCTCGGAGCCCAGAAACACAAGCCTT 60.1 0.40
mm4__chr7 131.69 Mbp_chr7_131687005_F TCGTCGGCAGCGTCTGAGGACCTAGCTCAGCTCA 59.6 0.39
mm4__chr7 131.69 Mbp_chr7_131687005_R GTCTCGTGGGCTCGGTCTCTTTGCTGGACACCACC 59.8 0.39
mm3__chr3 149.75 Mbp_chr3_149752477_F TCGTCGGCAGCGTCTGTCCTACGATTTCACCCGG 59.4 0.36
mm3__chr3 149.75 Mbp_chr3_149752477_R GTCTCGTGGGCTCGGCGTCCGAGTGTAACGCGTTA 60.4 0.36
mm4__chr5 14.57 Mbp_chr5_14567986_F Primer3: not found at this Tm N.d. 0.31
mm4__chr5 14.57 Mbp_chr5_14567986_R Primer3: not found at this Tm N.d. 0.31
mm3__chr7 157.43 Mbp_chr7_157428889_F TCGTCGGCAGCGTCCCTCTGGAGGGACCTGGATT 60.3 0.31
mm3__chr7 157.43 Mbp_chr7_157428889_R GTCTCGTGGGCTCGGGTGGGCAGATTTCCCAGTGA 59.9 0.31
mm4__chr19 4.72 Mbp_chr19_4723761_F TCGTCGGCAGCGTCCTCCATGCCCGGGACTGC 62.8 0.30
mm4__chr19 4.72 Mbp_chr19_4723761_R GTCTCGTGGGCTCGGAACTTCCGGGTCAAAGGTGC 60.8 0.30
mm4__chr16 73.12 Mbp_chr16_73116465_F Primer3: not found at this Tm N.d. 0.30
mm4__chr16 73.12 Mbp_chr16_73116465_R Primer3: not found at this Tm N.d. 0.30
mm4__chr1 22.38 Mbp_chr1_22376261_F Primer3: not found at this Tm N.d. 0.28
mm4__chr1 22.38 Mbp_chr1_22376261_R Primer3: not found at this Tm N.d. 0.28
mm4__chr16 13.42 Mbp_chr16_13424353_F TCGTCGGCAGCGTCTAATGACCTTGTGGCCAGCC 60.3 0.26
mm4__chr16 13.42 Mbp_chr16_13424353_R GTCTCGTGGGCTCGGGGAGCAGAAGAGACAGGCAG 60.1 0.26
mm4__chr19 18.93 Mbp_chr19_18928918_F TCGTCGGCAGCGTCGCCGAATGGGTGCAGCTATA 60.2 0.25
mm4__chr19 18.93 Mbp_chr19_18928918_R GTCTCGTGGGCTCGGGGACTTTCATGGCCCCTTCA 59.9 0.25
mm4__chr6 169.99 Mbp_chr6_169991358_F Primer3: not found at this Tm N.d. 0.24
mm4__chr6 169.99 Mbp_chr6_169991358_R Primer3: not found at this Tm N.d. 0.24
mm4__chr20 34.87 Mbp_chr20_34872848_F TCGTCGGCAGCGTCCTGGCTGGCCTCGTTCTC 60.1 0.24
mm4__chr20 34.87 Mbp_chr20_34872848_R GTCTCGTGGGCTCGGGGCTGTCGCGAAGATCAGG 60.8 0.24
mm3__chr16 81.04 Mbp_chr16_81035809_F Primer3: not found at this Tm N.d. 0.24
mm3__chr16 81.04 Mbp_chr16_81035809_R Primer3: not found at this Tm N.d. 0.24
mm4__chr5 179.19 Mbp_chr5_179191338_F TCGTCGGCAGCGTCGGGCTCAGTGAGGAGTTTCC 60.0 0.24
mm4__chr5 179.19 Mbp_chr5_179191338_R GTCTCGTGGGCTCGGCACACAGCCTCTGAGACGG 60.0 0.24
mm4__chr17 27.62 Mbp_chr17_27617394_F TCGTCGGCAGCGTCAACTTCCCAAGCTGAACCGG 60.5 0.24
mm4__chr17 27.62 Mbp_chr17_27617394_R GTCTCGTGGGCTCGGCCCTCTAAGACCCCAGGTGA 59.9 0.24
mm4__chr2 47.59 Mbp_chr2_47594221_F Primer3: not found at this Tm N.d. 0.23
mm4__chr2 47.59 Mbp_chr2_47594221_R Primer3: not found at this Tm N.d. 0.23
mm4__chr7 2.01 Mbp_chr7_2014866_F TCGTCGGCAGCGTCGAGTTCAGCATCCCCAGACC 60.1 0.23
mm4__chr7 2.01 Mbp_chr7_2014866_R GTCTCGTGGGCTCGGGACCCCATAGCCATACTGCC 59.9 0.23
mm2__chr12 57.09 Mbp_chr12_57085328_F Primer3: not found at this Tm N.d. 0.23
mm2__chr12 57.09 Mbp_chr12_57085328_R Primer3: not found at this Tm N.d. 0.23
mm4__chrX 130.06 Mbp_chrX_130055027_F TCGTCGGCAGCGTCCTGACAAGACAGAGCCCAGG 60.0 0.23
mm4__chrX 130.06 Mbp_chrX_130055027_R GTCTCGTGGGCTCGGGGTCCCAGTTCACAGGGTTT 59.8 0.23
mm3__chr18 64.78 Mbp_chr18_64780866_F Primer3: not found at this Tm N.d. 0.22
mm3__chr18 64.78 Mbp_chr18_64780866_R Primer3: not found at this Tm N.d. 0.22
mm4__chr18 49.02 Mbp_chr18_49021662_F TCGTCGGCAGCGTCCCCATGACCTCACCTCTCCT 60.3 0.21
mm4__chr18 49.02 Mbp_chr18_49021662_R GTCTCGTGGGCTCGGTAGGCCCCTCCTCTGTTACC 60.0 0.21
mm4__chr11 110.71 Mbp_chr11_110711736_F TCGTCGGCAGCGTCCTGACTTTGGGGCTGACACC 60.8 0.21
mm4__chr11 110.71 Mbp_chr11_110711736_R GTCTCGTGGGCTCGGCTCCTCCCTCAGTCTAGGCG 60.8 0.21
mm4__chr17 74.37 Mbp_chr17_74368932_F TCGTCGGCAGCGTCGCTGCTCCCAGAGGATGC 60.2 0.21
mm4__chr17 74.37 Mbp_chr17_74368932_R GTCTCGTGGGCTCGGGGGCTATGATCCCAAGAGGC 59.9 0.21
mm4__chr2 232.88 Mbp_chr2_232878385_F TCGTCGGCAGCGTCAACTGAAGGTGTGAAGGGCC 60.1 0.21
mm4__chr2 232.88 Mbp_chr2_232878385_R GTCTCGTGGGCTCGGACACAGCTTTGGAAGACCGT 59.8 0.21
mm4__chr1 247.45 Mbp_chr1_247446187_F Primer3: not found at this Tm N.d. 0.21
mm4__chr1 247.45 Mbp_chr1_247446187_R Primer3: not found at this Tm N.d. 0.21
mm4__chr13 79.62 Mbp_chr13_79622838_F TCGTCGGCAGCGTCGGGTAGCTGAACACCAGAGG 59.7 0.20
mm4__chr13 79.62 Mbp_chr13_79622838_R GTCTCGTGGGCTCGGAGCTTCTTCATCACGGAACA 57.1 0.20
mm4__chr11 125.25 Mbp_chr11_125252148_F Primer3: not found at this Tm N.d. 0.20
mm4__chr11 125.25 Mbp_chr11_125252148_R Primer3: not found at this Tm N.d. 0.20
mm4__chr14 104.28 Mbp_chr14_104278635_F TCGTCGGCAGCGTCCCTGGTGGCTTGGGTAATGT 59.9 0.20
mm4__chr14 104.28 Mbp_chr14_104278635_R GTCTCGTGGGCTCGGACCGGAGACTTGAGGGGAAT 60.2 0.20
mm4__chr2 240.97 Mbp_chr2_240972340_F TCGTCGGCAGCGTCAATATGGGGAGCCAGCAGTG 59.8 0.19
mm4__chr2 240.97 Mbp_chr2_240972340_R GTCTCGTGGGCTCGGCATCCCTGTGAGGCAAGCA 60.0 0.19
mm4__chr10 88.90 Mbp_chr10_88897158_F TCGTCGGCAGCGTCGGGACACAAAAGCCAAGAGG 59.0 0.19
mm4__chr10 88.90 Mbp_chr10_88897158_R GTCTCGTGGGCTCGGGTGGCCAAAATGCTGTCTCA 59.0 0.19
mm4__chr11 68.97 Mbp_chr11_68967396_F TCGTCGGCAGCGTCTGCCTAGAGAACAGGCTGGA 60.2 0.18
mm4__chr11 68.97 Mbp_chr11_68967396_R GTCTCGTGGGCTCGGGCCCACTGACACCTACACTC 60.0 0.18
mm4__chr15 74.37 Mbp_chr15_74369590_F TCGTCGGCAGCGTCACTTGTGGTCCTCTTGGCAG 59.8 0.18
mm4__chr15 74.37 Mbp_chr15_74369590_R GTCTCGTGGGCTCGGGTGACCATGGCCATTGGAGA 60.0 0.18
mm4__chr10 106.24 Mbp_chr10_106237667_F Primer3: not found at this Tm N.d. 0.17
mm4__chr10 106.24 Mbp_chr10_106237667_R Primer3: not found at this Tm N.d. 0.17
mm4__chr11 69.48 Mbp_chr11_69475132_F TCGTCGGCAGCGTCAGACAGAAGCCTGAACCTGC 59.9 0.17
mm4__chr11 69.48 Mbp_chr11_69475132_R GTCTCGTGGGCTCGGCCTCAAGGAGGCAAGGAAGG 60.0 0.17
mm4__chrX 132.45 Mbp_chrX_132449981_F Primer3: not found at this Tm N.d. 0.17
mm4__chrX 132.45 Mbp_chrX_132449981_R Primer3: not found at this Tm N.d. 0.17
mm4__chr19 4.36 Mbp_chr19_4361924_F TCGTCGGCAGCGTCCTCAGACCTGCTGTGACCTG 60.0 0.17
mm4__chr19 4.36 Mbp_chr19_4361924_R GTCTCGTGGGCTCGGTTCTCCCATCCTCCCTCCTG 60.0 0.17
mm4__chr1 22.25 Mbp_chr1_22248671_F TCGTCGGCAGCGTCCATGAGAGTGGAAGCCCTGG 60.1 0.16
mm4__chr1 22.25 Mbp_chr1_22248671_R GTCTCGTGGGCTCGGCAGGATCAGATCGGGTCAGC 59.9 0.16
mm4__chr2 240.63 Mbp_chr2_240629867_F TCGTCGGCAGCGTCTTTGTGTTTGTGGGTGTGGG 59.1 0.15
mm4__chr2 240.63 Mbp_chr2_240629867_R GTCTCGTGGGCTCGGGTCAGCAGAGAGTGAGCAGG 60.1 0.15
mm4__chr9 133.47 Mbp_chr9_133472583_F TCGTCGGCAGCGTCGGACCCTCTAGGCTGATGGA 60.1 0.15
mm4__chr9 133.47 Mbp_chr9_133472583_R GTCTCGTGGGCTCGGAGGCCCAGAGTAAGTTCCCA 60.1 0.15
mm4__chr19 40.23 Mbp_chr19_40234625_F Primer3: not found at this Tm N.d. 0.15
mm4__chr19 40.23 Mbp_chr19_40234625_R Primer3: not found at this Tm N.d. 0.15
mm4__chr8 142.91 Mbp_chr8_142914987_F TCGTCGGCAGCGTCGATGCACTGCTGAGCACAAG 59.8 0.14
mm4__chr8 142.91 Mbp_chr8_142914987_R GTCTCGTGGGCTCGGATAGAAGGTGTGGGCCATGC 60.1 0.14
mm4__chr2 2.56 Mbp_chr2_2560320_F TCGTCGGCAGCGTCGCTTGTGCTGCATGTAGCTG 60.1 0.14
mm4__chr2 2.56 Mbp_chr2_2560320_R GTCTCGTGGGCTCGGCCAGGGAGGGTAACAGAGGA 59.9 0.14
mm4__chr21 36.99 Mbp_chr21_36992885_F Primer3: not found at this Tm N.d. 0.14
mm4__chr21 36.99 Mbp_chr21_36992885_R Primer3: not found at this Tm N.d. 0.14
mm4__chr11 67.67 Mbp_chr11_67665921_F TCGTCGGCAGCGTCCTCCAGGCACCTGACTCTTG 60.0 0.14
mm4__chr11 67.67 Mbp_chr11_67665921_R GTCTCGTGGGCTCGGGCACATTGCAGCAACTGGAT 59.7 0.14
mm4__chr12 55.73 Mbp_chr12_55733492_F Primer3: not found at this Tm N.d. 0.14
mm4__chr12 55.73 Mbp_chr12_55733492_R Primer3: not found at this Tm N.d. 0.14
mm4__chr16 1.37 Mbp_chr16_1366532_F TCGTCGGCAGCGTCCAGAGACAGGGTCAGGGAGA 59.9 0.13
mm4__chr16 1.37 Mbp_chr16_1366532_R GTCTCGTGGGCTCGGGCCCAGGGCTCTTAAGACAG 60.1 0.13
mm4__chr13 40.64 Mbp_chr13_40635786_F TCGTCGGCAGCGTCCAGGAAAGGCCTCTGGATGG 60.1 0.13
mm4__chr13 40.64 Mbp_chr13_40635786_R GTCTCGTGGGCTCGGGGCAGCAGGTAGAGTACAGC 60.1 0.13
mm4__chr9 136.12 Mbp_chr9_136118396_F Primer3: not found at this Tm N.d. 0.13
mm4__chr9 136.12 Mbp_chr9_136118396_R Primer3: not found at this Tm N.d. 0.13
mm4__chr15_KI270727v1_random 243.59 Kbp_chr15_KI270727v1_random_243593_F Primer3: not found at this Tm N.d. 0.13
mm4__chr15_KI270727v1_random 243.59 Kbp_chr15_KI270727v1_random_243593_R Primer3: not found at this Tm N.d. 0.13
mm4__chr15 20.94 Mbp_chr15_20942752_F Primer3: not found at this Tm N.d. 0.13
mm4__chr15 20.94 Mbp_chr15_20942752_R Primer3: not found at this Tm N.d. 0.13
mm4__chr10 77.04 Mbp_chr10_77038917_F TCGTCGGCAGCGTCGTGAGGAATCTGAGGCACTGT 59.7 0.13
mm4__chr10 77.04 Mbp_chr10_77038917_R GTCTCGTGGGCTCGGTCTTTCCACTGGCTCAGCAG 59.9 0.13
mm4__chr10 111.12 Mbp_chr10_111115738_F TCGTCGGCAGCGTCACAGCAATGAGGAGGTGCC 60.0 0.13
mm4__chr10 111.12 Mbp_chr10_111115738_R GTCTCGTGGGCTCGGTCACCCAGAGAGCCTTCTGA 59.8 0.13
mm4__chr2 169.75 Mbp_chr2_169748200_F Primer3: not found at this Tm N.d. 0.13
mm4__chr2 169.75 Mbp_chr2_169748200_R Primer3: not found at this Tm N.d. 0.13
mm3__chr15 60.86 Mbp_chr15_60860897_F Primer3: not found at this Tm N.d. 0.13
mm3__chr15 60.86 Mbp_chr15_60860897_R Primer3: not found at this Tm N.d. 0.13
mm4__chr4 11.82 Mbp_chr4_11818761_F Primer3: not found at this Tm N.d. 0.13
mm4__chr4 11.82 Mbp_chr4_11818761_R Primer3: not found at this Tm N.d. 0.13
mm4__chr19 19.55 Mbp_chr19_19546006_F TCGTCGGCAGCGTCACTTGGTGACATCCGCAGG 60.0 0.12
mm4__chr19 19.55 Mbp_chr19_19546006_R GTCTCGTGGGCTCGGAAGGAGTCTGGAGGGAGGTG 60.2 0.12
mm4__chr16 74.30 Mbp_chr16_74297172_F TCGTCGGCAGCGTCGGCTGGTGACCCTTACTTGT 59.5 0.12
mm4__chr16 74.30 Mbp_chr16_74297172_R GTCTCGTGGGCTCGGTAAACGGAACAGGCAGCCAA 60.1 0.12
mm4__chrX 153.08 Mbp_chrX_153075591_F TCGTCGGCAGCGTCGGGGCAGACATCTTCAGGT 59.0 0.12
mm4__chrX 153.08 Mbp_chrX_153075591_R GTCTCGTGGGCTCGGCCTCCTCTCCTCCTCTCAGG 59.8 0.12
mm4__chr20 37.45 Mbp_chr20_37446618_F Primer3: not found at this Tm N.d. 0.12
mm4__chr20 37.45 Mbp_chr20_37446618_R Primer3: not found at this Tm N.d. 0.12
mm4__chr1 205.53 Mbp_chr1_205532679_F Primer3: not found at this Tm N.d. 0.12
mm4__chr1 205.53 Mbp_chr1_205532679_R Primer3: not found at this Tm N.d. 0.12
mm3__chr8 71.82 Mbp_chr8_71819187_F TCGTCGGCAGCGTCGGGCTCTGGCAAGGAATG 58.0 0.11
mm3__chr8 71.82 Mbp_chr8_71819187_R GTCTCGTGGGCTCGGCTCCACTCACACTGCCAGG 60.0 0.11
mm4__chr7 69.24 Mbp_chr7_69235994_F Primer3: not found at this Tm N.d. 0.11
mm4__chr7 69.24 Mbp_chr7_69235994_R Primer3: not found at this Tm N.d. 0.11
mm4__chr7 56.15 Mbp_chr7_56154018_F Primer3: not found at this Tm N.d. 0.11
mm4__chr7 56.15 Mbp_chr7_56154018_R Primer3: not found at this Tm N.d. 0.11
mm4__chr22 39.59 Mbp_chr22_39594601_F TCGTCGGCAGCGTCAGGAGGAGCTGAGAAGAGGC 60.6 0.11
mm4__chr22 39.59 Mbp_chr22_39594601_R GTCTCGTGGGCTCGGAGAGGAGTGGGAGAGGTGAC 59.9 0.11
mm4__chr11 455.89 Kbp_chr11_455892_F TCGTCGGCAGCGTCACTGGAAGGAAGGCTCAGGA 60.1 0.11
mm4__chr11 455.89 Kbp_chr11_455892_R GTCTCGTGGGCTCGGACTGTGAAGGTCATGCAGCA 59.8 0.11
mm4__chr20 37.41 Mbp_chr20_37406610_F TCGTCGGCAGCGTCGTGGATGTGGGTGGGAACAG 60.6 0.11
mm4__chr20 37.41 Mbp_chr20_37406610_R GTCTCGTGGGCTCGGCTTTGTCCCGGTTGCGCTC 61.9 0.11
mm3__chr5 443.16 Kbp_chr5_443160_F Primer3: not found at this Tm N.d. 0.11
mm3__chr5 443.16 Kbp_chr5_443160_R Primer3: not found at this Tm N.d. 0.11
mm4__chr15 31.81 Mbp_chr15_31813006_F TCGTCGGCAGCGTCCGCACACACACACAGGTATG 59.4 0.11
mm4__chr15 31.81 Mbp_chr15_31813006_R GTCTCGTGGGCTCGGCGCATCAGGTGTCTTGCTTC 59.5 0.11
mm4__chr9 129.75 Mbp_chr9_129748618_F TCGTCGGCAGCGTCGTCCCCTGTGTGCAGAAGAA 59.8 0.11
mm4__chr9 129.75 Mbp_chr9_129748618_R GTCTCGTGGGCTCGGCAGTATTGCAGGAGCGACCC 60.8 0.11
mm3__chr16 10.84 Mbp_chr16_10835453_F TCGTCGGCAGCGTCATAACAGACCAGCCAGCCAC 60.0 0.10
mm3__chr16 10.84 Mbp_chr16_10835453_R GTCTCGTGGGCTCGGCAGGATTCATGGCCCTCTCC 59.8 0.10
mm3__chr3 14.52 Mbp_chr3_14517814_F TCGTCGGCAGCGTCTACATCGGCTTTGGTCCTGG 59.7 0.10
mm3__chr3 14.52 Mbp_chr3_14517814_R GTCTCGTGGGCTCGGAAGTGCAGAGGAGTGAGCAG 59.6 0.10
mm4__chr17 76.74 Mbp_chr17_76736607_F Primer3: not found at this Tm N.d. 0.10
mm4__chr17 76.74 Mbp_chr17_76736607_R Primer3: not found at this Tm N.d. 0.10
mm4__chr10 129.74 Mbp_chr10_129741738_F TCGTCGGCAGCGTCAGCTTTCTCATGCATCCCGT 59.7 0.10
mm4__chr10 129.74 Mbp_chr10_129741738_R GTCTCGTGGGCTCGGGCACCCAAATGCTGACACAG 60.0 0.10
mm4__chr3 99.82 Mbp_chr3_99818097_F Primer3: not found at this Tm N.d. 0.10
mm4__chr3 99.82 Mbp_chr3_99818097_R Primer3: not found at this Tm N.d. 0.10
mm4__chr8 42.39 Mbp_chr8_42392081_F Primer3: not found at this Tm N.d. 0.10
mm4__chr8 42.39 Mbp_chr8_42392081_R Primer3: not found at this Tm N.d. 0.10
mm4__chr16 88.73 Mbp_chr16_88726525_F TCGTCGGCAGCGTCTCCATCATCTCCTTGGCTGC 59.8 0.10
mm4__chr16 88.73 Mbp_chr16_88726525_R GTCTCGTGGGCTCGGTATGCACCGTCAAGGGCTAC 59.8 0.10
mm4__chr1 119.09 Mbp_chr1_119087816_F Primer3: not found at this Tm N.d. 0.10
mm4__chr1 119.09 Mbp_chr1_119087816_R Primer3: not found at this Tm N.d. 0.10
mm4__chr12 109.83 Mbp_chr12_109828322_F TCGTCGGCAGCGTCTCCTGTGCGCCTGGTGTG 62.6 0.09
mm4__chr12 109.83 Mbp_chr12_109828322_R GTCTCGTGGGCTCGGCCTGAGCATTGGTTTCCCCT 59.9 0.09
mm4__chr15 75.29 Mbp_chr15_75294388_F TCGTCGGCAGCGTCAGTGAGCCTGGACAACAACG 60.5 0.09
mm4__chr15 75.29 Mbp_chr15_75294388_R GTCTCGTGGGCTCGGATGACAGGAGACAGCTGCAC 60.0 0.09
mm4__chr7 31.07 Mbp_chr7_31070366_F TCGTCGGCAGCGTCCCCAGAAACCTTCCCTGCAT 59.9 0.09
mm4__chr7 31.07 Mbp_chr7_31070366_R GTCTCGTGGGCTCGGCCTCCAAGTCTGCTCTCTGC 60.1 0.09
mm4__chr7 35.98 Mbp_chr7_35982717_F TCGTCGGCAGCGTCCTTCCGAGGGCCCAGTAAAG 60.1 0.09
mm4__chr7 35.98 Mbp_chr7_35982717_R GTCTCGTGGGCTCGGCGGCTACGGAATCCTAGGGT 60.8 0.09
mm4__chr1 2.92 Mbp_chr1_2923105_F TCGTCGGCAGCGTCGGGCCCTGGTTACCTCTAAC 59.4 0.09
mm4__chr1 2.92 Mbp_chr1_2923105_R GTCTCGTGGGCTCGGGGTCCCTAGAGACAGACCGT 60.0 0.09
mm4__chr22 46.00 Mbp_chr22_45995919_F Primer3: not found at this Tm N.d. 0.08
mm4__chr22 46.00 Mbp_chr22_45995919_R Primer3: not found at this Tm N.d. 0.08
mm4__chr18 71.86 Mbp_chr18_71858374_F Primer3: not found at this Tm N.d. 0.08
mm4__chr18 71.86 Mbp_chr18_71858374_R Primer3: not found at this Tm N.d. 0.08
mm4__chr6 1.54 Mbp_chr6_1536615_F Primer3: not found at this Tm N.d. 0.08
mm4__chr6 1.54 Mbp_chr6_1536615_R Primer3: not found at this Tm N.d. 0.08
mm4__chr16 57.10 Mbp_chr16_57103553_F TCGTCGGCAGCGTCCAGGACATCCCACTGTGGTC 60.0 0.08
mm4__chr16 57.10 Mbp_chr16_57103553_R GTCTCGTGGGCTCGGGAAGGGGAGAAGAGCTGCTG 60.1 0.08
mm4__chr7 165.54 Kbp_chr7_165545_F TCGTCGGCAGCGTCTGATGTCTCGTGATCATTTCTCT 57.4 0.08
mm4__chr7 165.54 Kbp_chr7_165545_R GTCTCGTGGGCTCGGGAACCAGCCCAGGTCTCG 59.7 0.08
mm3__chr14 91.27 Mbp_chr14_91272976_F TCGTCGGCAGCGTCGGTGAGAAGTGCAGGAGCTG 60.6 0.08
mm3__chr14 91.27 Mbp_chr14_91272976_R GTCTCGTGGGCTCGGCCTGCATCAGTCTGCCACA 60.0 0.08
mm3__chr11 557.07 Kbp_chr11_557071_F TCGTCGGCAGCGTCACTCCACGGTCTTGTGATGC 60.3 0.08
mm3__chr11 557.07 Kbp_chr11_557071_R GTCTCGTGGGCTCGGATCAAGCAGAGCGTGGTCC 60.0 0.08
mm4__chr13 50.20 Mbp_chr13_50201232_F Primer3: not found at this Tm N.d. 0.08
mm4__chr13 50.20 Mbp_chr13_50201232_R Primer3: not found at this Tm N.d. 0.08
mm4__chr1 216.87 Mbp_chr1_216874123_F Primer3: not found at this Tm N.d. 0.07
mm4__chr1 216.87 Mbp_chr1_216874123_R Primer3: not found at this Tm N.d. 0.07
mm4__chr8 142.88 Mbp_chr8_142876964_F TCGTCGGCAGCGTCCTCCCCACACTCCCTTCAGT 60.8 0.07
mm4__chr8 142.88 Mbp_chr8_142876964_R GTCTCGTGGGCTCGGTGGGAAGATCCAGCCTCAGA 59.9 0.07
mm4__chr7 44.23 Mbp_chr7_44229414_F TCGTCGGCAGCGTCAGCAGGACCCAGGCTACTTA 59.9 0.07
mm4__chr7 44.23 Mbp_chr7_44229414_R GTCTCGTGGGCTCGGCCGACATCCTGAGCTCAGTG 60.1 0.07
mm4__chr20 63.36 Mbp_chr20_63362410_F Primer3: not found at this Tm N.d. 0.07
mm4__chr20 63.36 Mbp_chr20_63362410_R Primer3: not found at this Tm N.d. 0.07
mm3__chr19 439.20 Kbp_chr19_439201_F Primer3: not found at this Tm N.d. 0.07
mm3__chr19 439.20 Kbp_chr19_439201_R Primer3: not found at this Tm N.d. 0.07
mm4__chr22 32.92 Mbp_chr22_32917909_F Primer3: not found at this Tm N.d. 0.07
mm4__chr22 32.92 Mbp_chr22_32917909_R Primer3: not found at this Tm N.d. 0.07
mm4__chr17 74.92 Mbp_chr17_74923077_F TCGTCGGCAGCGTCTGGTACTGGTCGTTCATGGC 60.0 0.07
mm4__chr17 74.92 Mbp_chr17_74923077_R GTCTCGTGGGCTCGGCCTCCAACCTCATGCCTCAG 60.1 0.07
mm4__chr2 241.85 Mbp_chr2_241854305_F Primer3: not found at this Tm N.d. 0.07
mm4__chr2 241.85 Mbp_chr2_241854305_R Primer3: not found at this Tm N.d. 0.07
mm3__chr1 204.69 Mbp_chr1_204685017_F TCGTCGGCAGCGTCCCCACTCTACCCAGCAACAG 60.0 0.07
mm3__chr1 204.69 Mbp_chr1_204685017_R GTCTCGTGGGCTCGGGGAGACTTGGGAGAGGGAGG 60.6 0.07
mm4__chr15 80.70 Mbp_chr15_80695057_F Primer3: not found at this Tm N.d. 0.06
mm4__chr15 80.70 Mbp_chr15_80695057_R Primer3: not found at this Tm N.d. 0.06
mm2__chr10 85.79 Mbp_chr10_85787237_F TCGTCGGCAGCGTCCAGTCCTGGAAGGGTTTGGT 59.5 0.06
mm2__chr10 85.79 Mbp_chr10_85787237_R GTCTCGTGGGCTCGGGAGTGCATGGCCAAGATGGA 60.3 0.06
mm4__chr11 64.91 Mbp_chr11_64913419_F Primer3: not found at this Tm N.d. 0.06
mm4__chr11 64.91 Mbp_chr11_64913419_R Primer3: not found at this Tm N.d. 0.06
mm4__chr19 11.19 Mbp_chr19_11193115_F TCGTCGGCAGCGTCCTACCACCCGGACTGTATCC 58.9 0.06
mm4__chr19 11.19 Mbp_chr19_11193115_R GTCTCGTGGGCTCGGGGAGACCCAGCTCAAGAAGG 59.7 0.06
mm4__chr8 144.10 Mbp_chr8_144104974_F TCGTCGGCAGCGTCGCTGCCTCCTGGGATTTGTA 59.7 0.06
mm4__chr8 144.10 Mbp_chr8_144104974_R GTCTCGTGGGCTCGGTGCCTAGTTTCTCCAGACGC 59.7 0.06
mm4__chr7 157.28 Mbp_chr7_157276311_F TCGTCGGCAGCGTCCGTGCAGCAATGATGTGTGT 59.7 0.06
mm4__chr7 157.28 Mbp_chr7_157276311_R GTCTCGTGGGCTCGGCGGATCAGGAAACAGGTCCC 60.1 0.06
mm4__chr7 2.48 Mbp_chr7_2477210_F TCGTCGGCAGCGTCGGGAGGTGAGCAGAGAGAGT 60.3 0.05
mm4__chr7 2.48 Mbp_chr7_2477210_R GTCTCGTGGGCTCGGCACCTGCAGCAGCCCTAG 60.1 0.05
mm4__chr5 5.39 Mbp_chr5_5389724_F Primer3: not found at this Tm N.d. 0.05
mm4__chr5 5.39 Mbp_chr5_5389724_R Primer3: not found at this Tm N.d. 0.05
mm4__chr2 231.90 Mbp_chr2_231900688_F TCGTCGGCAGCGTCGAGAGCACGGACTTCCCTTC 60.1 0.05
mm4__chr2 231.90 Mbp_chr2_231900688_R GTCTCGTGGGCTCGGTCTACCGTCCCCTCTGATCC 59.8 0.05
mm4__chr3 23.71 Mbp_chr3_23705376_F TCGTCGGCAGCGTCTAGAGGCAGGATGCTGGAGA 59.7 0.05
mm4__chr3 23.71 Mbp_chr3_23705376_R GTCTCGTGGGCTCGGGTCCCCAGGCATTGAACTGA 59.9 0.05
mm4__chr8 23.20 Mbp_chr8_23199935_F TCGTCGGCAGCGTCGGTCCCCTCCACAACCTAGA 60.2 0.05
mm4__chr8 23.20 Mbp_chr8_23199935_R GTCTCGTGGGCTCGGTCTTTGTCCAGTGTCCCAGC 59.8 0.05
mm4__chr12 129.10 Mbp_chr12_129097095_F Primer3: not found at this Tm N.d. 0.05
mm4__chr12 129.10 Mbp_chr12_129097095_R Primer3: not found at this Tm N.d. 0.05
mm4__chr1 72.42 Mbp_chr1_72419402_F TCGTCGGCAGCGTCTTCTTAGCCAGCGTGGAGTG 60.0 0.05
mm4__chr1 72.42 Mbp_chr1_72419402_R GTCTCGTGGGCTCGGCTGAAGGTAGGAATGGGCCG 60.1 0.05
mm4__chr4 94.92 Mbp_chr4_94916838_F Primer3: not found at this Tm N.d. 0.05
mm4__chr4 94.92 Mbp_chr4_94916838_R Primer3: not found at this Tm N.d. 0.05
mm4__chr1 18.87 Mbp_chr1_18869287_F TCGTCGGCAGCGTCATCCGGCCTCAGTCAAATGG 60.1 0.05
mm4__chr1 18.87 Mbp_chr1_18869287_R GTCTCGTGGGCTCGGGACCTGAGGCCTCTTTGCAT 60.0 0.05
mm4__chr20 51.51 Mbp_chr20_51506885_F TCGTCGGCAGCGTCAGCAGATGTCTCCTTTCCCA 58.3 0.05
mm4__chr20 51.51 Mbp_chr20_51506885_R GTCTCGTGGGCTCGGCCTGGCTGGCCATGTGAC 60.7 0.05
mm4__chr2 112.63 Mbp_chr2_112626771_F TCGTCGGCAGCGTCTGCTTAGGAGACGGGACAGA 59.9 0.05
mm4__chr2 112.63 Mbp_chr2_112626771_R GTCTCGTGGGCTCGGCCTCCTGGTGCATCCTGTTT 59.9 0.05
mm4__chr12 52.38 Mbp_chr12_52377352_F TCGTCGGCAGCGTCCTCCTTGGAGCTTGCAGACA 59.9 0.04
mm4__chr12 52.38 Mbp_chr12_52377352_R GTCTCGTGGGCTCGGACTCCAGCATGCAAGTCCAC 60.6 0.04
mm3__chr9 19.10 Mbp_chr9_19101274_F Primer3: not found at this Tm N.d. 0.04
mm3__chr9 19.10 Mbp_chr9_19101274_R Primer3: not found at this Tm N.d. 0.04
mm4__chrX 71.12 Mbp_chrX_71118792_F TCGTCGGCAGCGTCTCTTGAGCTACGAACACCGG 59.7 0.04
mm4__chrX 71.12 Mbp_chrX_71118792_R GTCTCGTGGGCTCGGCCTCCTTCTGTTTGGGGTCC 59.9 0.04
mm4__chr15 70.49 Mbp_chr15_70493967_F Primer3: not found at this Tm N.d. 0.04
mm4__chr15 70.49 Mbp_chr15_70493967_R Primer3: not found at this Tm N.d. 0.04
mm4__chr2 32.95 Mbp_chr2_32947903_F TCGTCGGCAGCGTCGGTCAGGGCCACTCGGAG 61.7 0.04
mm4__chr2 32.95 Mbp_chr2_32947903_R GTCTCGTGGGCTCGGGAGGAGAAAGCCGACCCAC 60.0 0.04
mm4__chr12 45.05 Mbp_chr12_45050718_F TCGTCGGCAGCGTCCAGCTTTAGGGGAACTGGGC 60.6 0.04
mm4__chr12 45.05 Mbp_chr12_45050718_R GTCTCGTGGGCTCGGGATCGAGAATTTGCTGCGGG 59.6 0.04
mm4__chr19 41.35 Mbp_chr19_41353690_F TCGTCGGCAGCGTCGGCAACGGAAAAGTCTCAAAAGT 60.1 0.04
mm4__chr19 41.35 Mbp_chr19_41353690_R GTCTCGTGGGCTCGGCTGAGGCCCCAGAGTCTGA 60.3 0.04
mm4__chr8 133.82 Mbp_chr8_133818159_F Primer3: not found at this Tm N.d. 0.04
mm4__chr8 133.82 Mbp_chr8_133818159_R Primer3: not found at this Tm N.d. 0.04
mm4__chr11 107.59 Mbp_chr11_107590659_F Primer3: not found at this Tm N.d. 0.04
mm4__chr11 107.59 Mbp_chr11_107590659_R Primer3: not found at this Tm N.d. 0.04
mm4__chr4 168.88 Mbp_chr4_168878151_F Primer3: not found at this Tm N.d. 0.04
mm4__chr4 168.88 Mbp_chr4_168878151_R Primer3: not found at this Tm N.d. 0.04
mm3__chr9 95.38 Mbp_chr9_95384073_F TCGTCGGCAGCGTCTGCCAGTGAAGAAGGCAACA 60.1 0.04
mm3__chr9 95.38 Mbp_chr9_95384073_R GTCTCGTGGGCTCGGACTCACAAGCATCCAGGACA 58.9 0.04
mm4__chr7 44.93 Mbp_chr7_44933484_F Primer3: not found at this Tm N.d. 0.03
mm4__chr7 44.93 Mbp_chr7_44933484_R Primer3: not found at this Tm N.d. 0.03
mm4__chr8 94.39 Mbp_chr8_94389238_F Primer3: not found at this Tm N.d. 0.03
mm4__chr8 94.39 Mbp_chr8_94389238_R Primer3: not found at this Tm N.d. 0.03
mm4__chr11 10.99 Mbp_chr11_10985036_F Primer3: not found at this Tm N.d. 0.03
mm4__chr11 10.99 Mbp_chr11_10985036_R Primer3: not found at this Tm N.d. 0.03
mm4__chr4 143.70 Mbp_chr4_143700664_F TCGTCGGCAGCGTCCGACTCAAGAGCAGGCAGG 60.4 0.03
mm4__chr4 143.70 Mbp_chr4_143700664_R GTCTCGTGGGCTCGGGGAATCCCAACGCCAGGAG 60.4 0.03
mm4__chr2 239.48 Mbp_chr2_239483082_F Primer3: not found at this Tm N.d. 0.03
mm4__chr2 239.48 Mbp_chr2_239483082_R Primer3: not found at this Tm N.d. 0.03
mm4__chr21 44.94 Mbp_chr21_44939916_F Primer3: not found at this Tm N.d. 0.03
mm4__chr21 44.94 Mbp_chr21_44939916_R Primer3: not found at this Tm N.d. 0.03
mm4__chr22 46.74 Mbp_chr22_46738218_F Primer3: not found at this Tm N.d. 0.03
mm4__chr22 46.74 Mbp_chr22_46738218_R Primer3: not found at this Tm N.d. 0.03
mm4__chr11 111.88 Mbp_chr11_111878890_F TCGTCGGCAGCGTCTCTCCACGTGGGTTACATCC 59.1 0.03
mm4__chr11 111.88 Mbp_chr11_111878890_R GTCTCGTGGGCTCGGCACTGGCCTGGGAGAATCTG 60.1 0.03
mm2__chr7 106.01 Mbp_chr7_106013485_F TCGTCGGCAGCGTCCCACACCGTAGAACCACTGG 60.3 0.03
mm2__chr7 106.01 Mbp_chr7_106013485_R GTCTCGTGGGCTCGGGGACCCACTTTTCATGCCCA 60.5 0.03
mm4__chr22 47.03 Mbp_chr22_47029869_F TCGTCGGCAGCGTCTCTGTCTTGAGCTGTCAGCC 59.6 0.03
mm4__chr22 47.03 Mbp_chr22_47029869_R GTCTCGTGGGCTCGGCTCACCAGCCAGAACACCAA 60.1 0.03
mm4__chr10 133.24 Mbp_chr10_133237059_F Primer3: not found at this Tm N.d. 0.03
mm4__chr10 133.24 Mbp_chr10_133237059_R Primer3: not found at this Tm N.d. 0.03
mm3__chr20 676.45 Kbp_chr20_676446_F TCGTCGGCAGCGTCCCTCCAGTCCTCTCCCTCTC 60.1 0.02
mm3__chr20 676.45 Kbp_chr20_676446_R GTCTCGTGGGCTCGGTGAACCCCAAACCCAACAGG 60.3 0.02
mm4__chr19 40.43 Mbp_chr19_40425623_F TCGTCGGCAGCGTCCCTGGCGGGCTGTTCTCT 61.7 0.02
mm4__chr19 40.43 Mbp_chr19_40425623_R GTCTCGTGGGCTCGGCTTCTTGGCTCCGGCTGC 61.1 0.02
mm4__chr3 122.91 Mbp_chr3_122913135_F TCGTCGGCAGCGTCGCTAGGCCTCAGCGACTG 59.8 0.02
mm4__chr3 122.91 Mbp_chr3_122913135_R GTCTCGTGGGCTCGGCCCTCCTTCCTCTCTCCCAG 60.3 0.02
mm4__chr18 12.88 Mbp_chr18_12883577_F TCGTCGGCAGCGTCCTGCTCCCACAGGTCCTG 59.3 0.02
mm4__chr18 12.88 Mbp_chr18_12883577_R GTCTCGTGGGCTCGGTTTCTCTGGCCGGGTGTTTT 60.1 0.02
mm4__chr1 2.25 Mbp_chr1_2247641_F Primer3: not found at this Tm N.d. 0.02
mm4__chr1 2.25 Mbp_chr1_2247641_R Primer3: not found at this Tm N.d. 0.02
mm4__chr17 81.09 Mbp_chr17_81085955_F TCGTCGGCAGCGTCACCTGTGGGTGAATCCGTTG 60.2 0.02
mm4__chr17 81.09 Mbp_chr17_81085955_R GTCTCGTGGGCTCGGGAACTGCAGAGGCCAGAGAA 59.6 0.02
mm4__chr15 31.39 Mbp_chr15_31391608_F TCGTCGGCAGCGTCGCCCCACACCCATTTCTGG 60.9 0.02
mm4__chr15 31.39 Mbp_chr15_31391608_R GTCTCGTGGGCTCGGAGTGCACCTAGAGCCGTTTC 60.0 0.02
mm4__chr22 40.06 Mbp_chr22_40058473_F Primer3: not found at this Tm N.d. 0.02
mm4__chr22 40.06 Mbp_chr22_40058473_R Primer3: not found at this Tm N.d. 0.02
mm4__chr3 128.49 Mbp_chr3_128489304_F Primer3: not found at this Tm N.d. 0.02
mm4__chr3 128.49 Mbp_chr3_128489304_R Primer3: not found at this Tm N.d. 0.02
mm4__chr15 94.23 Mbp_chr15_94231540_F TCGTCGGCAGCGTCTCCTCCCCTTTAGGCGGG 60.3 0.02
mm4__chr15 94.23 Mbp_chr15_94231540_R GTCTCGTGGGCTCGGCAGTCGCCACTCGGAAGC 60.8 0.02
mm3__chr10 123.75 Mbp_chr10_123752828_F Primer3: not found at this Tm N.d. 0.02
mm3__chr10 123.75 Mbp_chr10_123752828_R Primer3: not found at this Tm N.d. 0.02
mm3__chr20 23.24 Mbp_chr20_23241765_F TCGTCGGCAGCGTCGGTCTGAGGGAGGAAGAGGA 59.6 0.02
mm3__chr20 23.24 Mbp_chr20_23241765_R GTCTCGTGGGCTCGGGCAAGGGCAGTGAAAGTGTG 59.9 0.02
mm4__chr12 131.93 Mbp_chr12_131929974_F Primer3: not found at this Tm N.d. 0.02
mm4__chr12 131.93 Mbp_chr12_131929974_R Primer3: not found at this Tm N.d. 0.02
mm4__chr18 13.32 Mbp_chr18_13319250_F Primer3: not found at this Tm N.d. 0.01
mm4__chr18 13.32 Mbp_chr18_13319250_R Primer3: not found at this Tm N.d. 0.01
mm4__chr19 7.91 Mbp_chr19_7912603_F TCGTCGGCAGCGTCACTGAGGACAGAGAGTGGGG 60.2 0.01
mm4__chr19 7.91 Mbp_chr19_7912603_R GTCTCGTGGGCTCGGCCTGCTGTCTTCCGTTCACA 60.2 0.01
mm4__chr3 98.02 Mbp_chr3_98024574_F TCGTCGGCAGCGTCGCCTATTGTGCTTCCCTTGG 58.8 0.01
mm4__chr3 98.02 Mbp_chr3_98024574_R GTCTCGTGGGCTCGGGGGTGAGAAGAAAGATCCTCTGG 60.1 0.01
mm4__chr16 73.06 Mbp_chr16_73057985_F TCGTCGGCAGCGTCGGTGGGGCAAAGTTGGGG 60.9 0.01
mm4__chr16 73.06 Mbp_chr16_73057985_R GTCTCGTGGGCTCGGGGTACCAGCTCGGAGACAAA 59.3 0.01
mm4__chr9 134.37 Mbp_chr9_134370325_F TCGTCGGCAGCGTCCAGTGTGACCTCGGGCAG 60.0 0.01
mm4__chr9 134.37 Mbp_chr9_134370325_R GTCTCGTGGGCTCGGCCAGAAAGGCCTTGGAACCA 60.1 0.01
mm4__chr7 46.65 Mbp_chr7_46648891_F Primer3: not found at this Tm N.d. 0.01
mm4__chr7 46.65 Mbp_chr7_46648891_R Primer3: not found at this Tm N.d. 0.01
mm4__chr3 140.17 Mbp_chr3_140174208_F Primer3: not found at this Tm N.d. 0.01
mm4__chr3 140.17 Mbp_chr3_140174208_R Primer3: not found at this Tm N.d. 0.01
mm4__chr19 58.10 Mbp_chr19_58096609_F TCGTCGGCAGCGTCAGAGTCCTGCAGCAAATCCC 60.0 0.01
mm4__chr19 58.10 Mbp_chr19_58096609_R GTCTCGTGGGCTCGGGGATGGAGAGCTGCGATGAA 59.8 0.01
mm4__chr11 62.15 Mbp_chr11_62145889_F TCGTCGGCAGCGTCACCCAGACCATCTTGTTGCT 59.2 0.01
mm4__chr11 62.15 Mbp_chr11_62145889_R GTCTCGTGGGCTCGGAGAGGCAGATTCTGGTCACC 59.0 0.01
mm4__chr15 31.39 Mbp_chr15_31392830_F TCGTCGGCAGCGTCCCCCACACCCATTTCTGTGA 59.8 0.01
mm4__chr15 31.39 Mbp_chr15_31392830_R GTCTCGTGGGCTCGGAGTGCACCTAGAGCCGTTTC 60.0 0.01
mm4__chr3 142.96 Mbp_chr3_142963265_F Primer3: not found at this Tm N.d. 0.01
mm4__chr3 142.96 Mbp_chr3_142963265_R Primer3: not found at this Tm N.d. 0.01
mm4__chr11 69.00 Mbp_chr11_69004487_F TCGTCGGCAGCGTCAACTTCCGTTGGCCACATTG 59.3 0.01
mm4__chr11 69.00 Mbp_chr11_69004487_R GTCTCGTGGGCTCGGGCTCCTGGACAGCCTCTTG 60.0 0.01
mm4__chr6 53.53 Mbp_chr6_53529782_F TCGTCGGCAGCGTCCCTGATCCCCAGACAGAAGC 59.8 0.01
mm4__chr6 53.53 Mbp_chr6_53529782_R GTCTCGTGGGCTCGGACCGCTGCAGAAATTCTCCA 59.9 0.01
mm3__chr3 50.37 Mbp_chr3_50371930_F TCGTCGGCAGCGTCGACAACTCAAGCCAGTGGGA 59.8 0.01
mm3__chr3 50.37 Mbp_chr3_50371930_R GTCTCGTGGGCTCGGGTCCAGCCTAGTCCAGCAAA 59.6 0.01
mm3__chr20 20.39 Mbp_chr20_20388362_F TCGTCGGCAGCGTCAACCATGTCCATATCTGAGAAGT 57.0 0.01
mm3__chr20 20.39 Mbp_chr20_20388362_R GTCTCGTGGGCTCGGTGCGTTTCTGCCACTCACTA 59.6 0.01
mm4__chr17 46.82 Mbp_chr17_46819903_F TCGTCGGCAGCGTCGCCCTGGGTGGTATTGACAT 59.7 0.01
mm4__chr17 46.82 Mbp_chr17_46819903_R GTCTCGTGGGCTCGGGTGCTTCCTTCACCCCAGAC 60.6 0.01
mm4__chr18 23.45 Mbp_chr18_23453176_F TCGTCGGCAGCGTCGCTGATGCTTGCTTCTCCGA 60.7 0.01
mm4__chr18 23.45 Mbp_chr18_23453176_R GTCTCGTGGGCTCGGTTCGCTAGGACAGTCGCTTG 60.1 0.01
mm4__chr3 13.93 Mbp_chr3_13933043_F TCGTCGGCAGCGTCGGAGCCCTGAGTCCGGAG 61.1 0.01
mm4__chr3 13.93 Mbp_chr3_13933043_R GTCTCGTGGGCTCGGGAATCCTGGGAAGCTCGTCC 60.1 0.01
mm4__chr22 44.33 Mbp_chr22_44331460_F TCGTCGGCAGCGTCCCTTTCCCCGCCGCTCTC 62.8 0.01
mm4__chr22 44.33 Mbp_chr22_44331460_R GTCTCGTGGGCTCGGGCTACGAAGCGAGGAACGAG 60.8 0.01
mm4__chr7 96.28 Mbp_chr7_96284631_F Primer3: not found at this Tm N.d. 0.01
mm4__chr7 96.28 Mbp_chr7_96284631_R Primer3: not found at this Tm N.d. 0.01
mm4__chr9 136.36 Mbp_chr9_136361604_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 136.36 Mbp_chr9_136361604_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 119.96 Mbp_chr12_119960044_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 119.96 Mbp_chr12_119960044_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 46.07 Mbp_chr22_46071409_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 46.07 Mbp_chr22_46071409_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 171.52 Mbp_chr2_171515798_F TCGTCGGCAGCGTCCAAAGGAAGAGGCTATGGACCA 59.7 0.00
mm4__chr2 171.52 Mbp_chr2_171515798_R GTCTCGTGGGCTCGGCTGTGGGGATACTGGCTGTG 60.1 0.00
mm4__chr11 65.63 Mbp_chr11_65634071_F TCGTCGGCAGCGTCGCCTCTGAACCTCCATGCTT 60.0 0.00
mm4__chr11 65.63 Mbp_chr11_65634071_R GTCTCGTGGGCTCGGTGAGCGGCTCACGTAGTAGA 60.3 0.00
mm4__chr4 1.63 Mbp_chr4_1625853_F TCGTCGGCAGCGTCCCAGGTGATCAGAAGGCCC 59.7 0.00
mm4__chr4 1.63 Mbp_chr4_1625853_R GTCTCGTGGGCTCGGCCTCTGGGGTCTCTGGACAG 60.9 0.00
mm4__chr16 26.55 Mbp_chr16_26547167_F TCGTCGGCAGCGTCTGGCTTCATGGTTTGGCAGA 60.1 0.00
mm4__chr16 26.55 Mbp_chr16_26547167_R GTCTCGTGGGCTCGGAGCCTGGAGAGGATAGGATTT 57.6 0.00
mm4__chr19 50.94 Mbp_chr19_50943407_F TCGTCGGCAGCGTCAAAGTGCCCCAGGGAGATTG 59.9 0.00
mm4__chr19 50.94 Mbp_chr19_50943407_R GTCTCGTGGGCTCGGCTGGACTCAGGGTCTGCTTC 59.7 0.00
mm4__chrX 35.81 Mbp_chrX_35808622_F Primer3: not found at this Tm N.d. 0.00
mm4__chrX 35.81 Mbp_chrX_35808622_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 23.03 Mbp_chr21_23030178_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 23.03 Mbp_chr21_23030178_R Primer3: not found at this Tm N.d. 0.00
mm4__chrX 119.61 Mbp_chrX_119605881_F TCGTCGGCAGCGTCCACTTTCGTGGCTATAGCGC 59.4 0.00
mm4__chrX 119.61 Mbp_chrX_119605881_R GTCTCGTGGGCTCGGCTGGAGGACGTCTGCTGTTG 60.6 0.00
mm4__chr9 134.40 Mbp_chr9_134404971_F TCGTCGGCAGCGTCTGCCAAGGATCTGAAAGGGG 59.6 0.00
mm4__chr9 134.40 Mbp_chr9_134404971_R GTCTCGTGGGCTCGGCCATTGTTGCTTCGCTGGAG 59.8 0.00
mm4__chr4 173.33 Mbp_chr4_173333717_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 173.33 Mbp_chr4_173333717_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 123.58 Mbp_chr4_123584302_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 123.58 Mbp_chr4_123584302_R Primer3: not found at this Tm N.d. 0.00
mm4__chr13 113.08 Mbp_chr13_113080728_F TCGTCGGCAGCGTCGGATCCAGTGTGGTGGGTG 60.0 0.00
mm4__chr13 113.08 Mbp_chr13_113080728_R GTCTCGTGGGCTCGGCGTGGTGGGTATCTGTGTGG 60.3 0.00
mm4__chr17 80.47 Mbp_chr17_80469592_F TCGTCGGCAGCGTCCACTGCTCAGGTGTCAGTGT 59.8 0.00
mm4__chr17 80.47 Mbp_chr17_80469592_R GTCTCGTGGGCTCGGCTCCAGAGACCTCACGTGTG 59.7 0.00
mm4__chr12 41.19 Mbp_chr12_41188864_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 41.19 Mbp_chr12_41188864_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 4.40 Mbp_chr19_4400236_F TCGTCGGCAGCGTCAACGTCCCCACCTCGGTC 61.3 0.00
mm4__chr19 4.40 Mbp_chr19_4400236_R GTCTCGTGGGCTCGGCAGTTCTACAAGGCGAGCCA 60.0 0.00
mm4__chrX 143.63 Mbp_chrX_143634510_F TCGTCGGCAGCGTCCCACCCGAGACCTGTACAAC 60.0 0.00
mm4__chrX 143.63 Mbp_chrX_143634510_R GTCTCGTGGGCTCGGAAAGGATCCGGGTGTCCTTG 59.6 0.00
mm4__chr6 168.88 Mbp_chr6_168875240_F TCGTCGGCAGCGTCAGCAGGGGTAGAGGATGGAG 60.1 0.00
mm4__chr6 168.88 Mbp_chr6_168875240_R GTCTCGTGGGCTCGGGACTGTGCCTGTAGCTGACC 60.3 0.00
mm4__chr12 49.06 Mbp_chr12_49060400_F TCGTCGGCAGCGTCCCCTTCCCCTCTGGCCTC 61.0 0.00
mm4__chr12 49.06 Mbp_chr12_49060400_R GTCTCGTGGGCTCGGGTTGGGCCATGCACCTCTAG 60.7 0.00
mm4__chr9 133.38 Mbp_chr9_133376656_F TCGTCGGCAGCGTCGTCGCAACCCTGGAGCTAC 60.4 0.00
mm4__chr9 133.38 Mbp_chr9_133376656_R GTCTCGTGGGCTCGGCGGGTTCAACCTAGGAGCTG 60.1 0.00
mm4__chr3 166.35 Mbp_chr3_166349317_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 166.35 Mbp_chr3_166349317_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 37.34 Mbp_chr22_37335179_F TCGTCGGCAGCGTCGGGGCGTAAAGAAGGGTCAT 59.7 0.00
mm4__chr22 37.34 Mbp_chr22_37335179_R GTCTCGTGGGCTCGGGAGGCGAGCAGGTTCCTG 60.1 0.00
mm4__chr1 34.06 Mbp_chr1_34064426_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 34.06 Mbp_chr1_34064426_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 35.94 Mbp_chr6_35940233_F Primer3: not found at this Tm N.d. 0.00
mm4__chr6 35.94 Mbp_chr6_35940233_R Primer3: not found at this Tm N.d. 0.00
mm4__chrX 48.03 Mbp_chrX_48030524_F Primer3: not found at this Tm N.d. 0.00
mm4__chrX 48.03 Mbp_chrX_48030524_R Primer3: not found at this Tm N.d. 0.00
mm4__chrX 137.44 Mbp_chrX_137440967_F Primer3: not found at this Tm N.d. 0.00
mm4__chrX 137.44 Mbp_chrX_137440967_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 93.58 Mbp_chr9_93577042_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 93.58 Mbp_chr9_93577042_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 127.86 Mbp_chr9_127857940_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 127.86 Mbp_chr9_127857940_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 125.68 Mbp_chr9_125681298_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 125.68 Mbp_chr9_125681298_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 122.91 Mbp_chr9_122905108_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 122.91 Mbp_chr9_122905108_R Primer3: not found at this Tm N.d. 0.00
mm4__chr8 63.14 Mbp_chr8_63144014_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 63.14 Mbp_chr8_63144014_R Primer3: not found at this Tm N.d. 0.00
mm4__chr8 28.27 Mbp_chr8_28267546_F TCGTCGGCAGCGTCGGCCAAGGAGCTAATGGAGG 60.1 0.00
mm4__chr8 28.27 Mbp_chr8_28267546_R GTCTCGTGGGCTCGGATCTTCGGTTGGAGCCTGTG 60.0 0.00
mm4__chr8 22.05 Mbp_chr8_22049062_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 22.05 Mbp_chr8_22049062_R Primer3: not found at this Tm N.d. 0.00
mm4__chr8 143.29 Mbp_chr8_143291348_F TCGTCGGCAGCGTCGGCAACCGATGAGCTGAAGG 61.3 0.00
mm4__chr8 143.29 Mbp_chr8_143291348_R GTCTCGTGGGCTCGGGAAACGCCAGTCTCCACCC 60.6 0.00
mm4__chr7 88.64 Mbp_chr7_88639743_F TCGTCGGCAGCGTCTTGAGGCACTGGACTGCATC 60.3 0.00
mm4__chr7 88.64 Mbp_chr7_88639743_R GTCTCGTGGGCTCGGGAACACCAGTCTCCACTCCG 60.0 0.00
mm4__chr7 6.53 Mbp_chr7_6531240_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 6.53 Mbp_chr7_6531240_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803453_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803453_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803414_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803414_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803378_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803378_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803303_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803303_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803264_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803264_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803225_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803225_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803000_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4803000_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 4.80 Mbp_chr7_4802889_F TCGTCGGCAGCGTCGTCATGCTGGCTGGACCC 60.4 0.00
mm4__chr7 4.80 Mbp_chr7_4802889_R GTCTCGTGGGCTCGGGGGACCCAGCCAGCATTC 60.4 0.00
mm4__chr7 4.80 Mbp_chr7_4802739_F TCGTCGGCAGCGTCGTACCTCGGGGCACGCTG 62.8 0.00
mm4__chr7 4.80 Mbp_chr7_4802739_R GTCTCGTGGGCTCGGGCATGACCCGAGGTGTCTG 60.4 0.00
mm4__chr7 4.80 Mbp_chr7_4802442_F TCGTCGGCAGCGTCGTACCTCGGGGCACGCTG 62.8 0.00
mm4__chr7 4.80 Mbp_chr7_4802442_R GTCTCGTGGGCTCGGGAGGTACCTGGGGAGGGG 60.0 0.00
mm4__chr7 4.80 Mbp_chr7_4802406_F TCGTCGGCAGCGTCGTACCTCGGGGCACGCTG 62.8 0.00
mm4__chr7 4.80 Mbp_chr7_4802406_R GTCTCGTGGGCTCGGGAGGTACCTGGGGAGGGG 60.0 0.00
mm4__chr7 2.15 Mbp_chr7_2152650_F TCGTCGGCAGCGTCAAGGAGATGGACCCATGGGA 59.9 0.00
mm4__chr7 2.15 Mbp_chr7_2152650_R GTCTCGTGGGCTCGGGTGAGCCTTAGGAAGCCCAG 60.1 0.00
mm4__chr7 2.02 Mbp_chr7_2016487_F TCGTCGGCAGCGTCGAGTGCACAAAACTGCCAGG 59.9 0.00
mm4__chr7 2.02 Mbp_chr7_2016487_R GTCTCGTGGGCTCGGCACTGGGTCGGTCTCTCCTA 60.0 0.00
mm4__chr7 158.59 Mbp_chr7_158588410_F TCGTCGGCAGCGTCAGGCTCGGTCCCAGGAAA 60.2 0.00
mm4__chr7 158.59 Mbp_chr7_158588410_R GTCTCGTGGGCTCGGAGGCCTCACCCTGCTCTG 61.0 0.00
mm4__chr7 110.15 Mbp_chr7_110149449_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 110.15 Mbp_chr7_110149449_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 87.32 Mbp_chr6_87322673_F TCGTCGGCAGCGTCGTGACCCCGCGAAAGGTAA 60.0 0.00
mm4__chr6 87.32 Mbp_chr6_87322673_R GTCTCGTGGGCTCGGCAGAGACGGGAGCGTGATG 60.2 0.00
mm4__chr6 6.81 Mbp_chr6_6810443_F TCGTCGGCAGCGTCGGGTTCTTGCCTACCACCTC 60.0 0.00
mm4__chr6 6.81 Mbp_chr6_6810443_R GTCTCGTGGGCTCGGAGAAGCCTTGAGATCCGCTG 59.8 0.00
mm4__chr6 36.84 Mbp_chr6_36840044_F Primer3: not found at this Tm N.d. 0.00
mm4__chr6 36.84 Mbp_chr6_36840044_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 35.21 Mbp_chr6_35214291_F TCGTCGGCAGCGTCCCAGGAGGAGCCGGTAGC 61.5 0.00
mm4__chr6 35.21 Mbp_chr6_35214291_R GTCTCGTGGGCTCGGGGATCCCAGCTCGCAGGA 61.1 0.00
mm4__chr5 3.43 Mbp_chr5_3429139_F TCGTCGGCAGCGTCCAGGGCTTCTCATGACCAGG 60.1 0.00
mm4__chr5 3.43 Mbp_chr5_3429139_R GTCTCGTGGGCTCGGAAAACATCACAGGCCAGGCT 60.1 0.00
mm4__chr5 174.77 Mbp_chr5_174767307_F TCGTCGGCAGCGTCCGTTGTGCTCCAAACTGACC 59.6 0.00
mm4__chr5 174.77 Mbp_chr5_174767307_R GTCTCGTGGGCTCGGAGAGGACCACAGAGCACTGA 60.1 0.00
mm4__chr5 172.59 Mbp_chr5_172594980_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 172.59 Mbp_chr5_172594980_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 7.65 Mbp_chr4_7654794_F TCGTCGGCAGCGTCTACTGGGTGCCAATGAGCAG 60.0 0.00
mm4__chr4 7.65 Mbp_chr4_7654794_R GTCTCGTGGGCTCGGGAGCCTCCTTTCTCGGGTCT 60.9 0.00
mm4__chr4 107.93 Mbp_chr4_107931828_F TCGTCGGCAGCGTCCTGCTATGCGGCCTCGTAG 60.3 0.00
mm4__chr4 107.93 Mbp_chr4_107931828_R GTCTCGTGGGCTCGGCAGCACGTGACCGAAGTTG 59.4 0.00
mm4__chr3 98.52 Mbp_chr3_98522623_F TCGTCGGCAGCGTCCCACCTCCTGGGCCTCAC 61.7 0.00
mm4__chr3 98.52 Mbp_chr3_98522623_R GTCTCGTGGGCTCGGGATTTGTCCCAGGGCCGAG 60.4 0.00
mm4__chr3 184.34 Mbp_chr3_184344901_F TCGTCGGCAGCGTCCCACTCTGCCCACCACTAAC 60.3 0.00
mm4__chr3 184.34 Mbp_chr3_184344901_R GTCTCGTGGGCTCGGAGGCATGATGTGGGTCACTG 60.0 0.00
mm4__chr22 43.21 Mbp_chr22_43207439_F TCGTCGGCAGCGTCCTCCTGCTCCTCTCCCATCA 60.3 0.00
mm4__chr22 43.21 Mbp_chr22_43207439_R GTCTCGTGGGCTCGGGGGAATCCACGTAACCCTGG 60.1 0.00
mm4__chr22 23.77 Mbp_chr22_23767515_F TCGTCGGCAGCGTCGTCGCCATCTGAGCCATGAG 60.8 0.00
mm4__chr22 23.77 Mbp_chr22_23767515_R GTCTCGTGGGCTCGGTTAACCCTGCTTCCTCCCAC 59.3 0.00
mm4__chr21 6.10 Mbp_chr21_6101636_F TCGTCGGCAGCGTCGTGGCCAGAGGGGAAGAATC 60.1 0.00
mm4__chr21 6.10 Mbp_chr21_6101636_R GTCTCGTGGGCTCGGCGTCCACCCTTCACTGAGTC 60.0 0.00
mm4__chr21 5.12 Mbp_chr21_5122032_F TCGTCGGCAGCGTCGCAGGAGGATCAGGAAGTGG 59.8 0.00
mm4__chr21 5.12 Mbp_chr21_5122032_R GTCTCGTGGGCTCGGCAGCGTCTGAGTCTCAGCAC 60.7 0.00
mm4__chr21 44.14 Mbp_chr21_44139997_F Primer3: not found at this Tm N.d. 0.00
mm4__chr21 44.14 Mbp_chr21_44139997_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 43.44 Mbp_chr21_43436599_F TCGTCGGCAGCGTCCGTCCACCCTTCACTGAGTC 60.0 0.00
mm4__chr21 43.44 Mbp_chr21_43436599_R GTCTCGTGGGCTCGGGTGGCCAGAGGGGAAGAATC 60.1 0.00
mm4__chr21 39.45 Mbp_chr21_39445650_F TCGTCGGCAGCGTCGACTGAGTGACCGGCGATAC 60.2 0.00
mm4__chr21 39.45 Mbp_chr21_39445650_R GTCTCGTGGGCTCGGCAGCCGGTTACCCTGTTACA 59.6 0.00
mm4__chr20 62.49 Mbp_chr20_62487804_F TCGTCGGCAGCGTCGCCTTATCTGGCCGCCTAAT 59.9 0.00
mm4__chr20 62.49 Mbp_chr20_62487804_R GTCTCGTGGGCTCGGTTCACTCCACCTCCCTCTCC 60.2 0.00
mm4__chr20 37.42 Mbp_chr20_37420185_F TCGTCGGCAGCGTCTCAGAAACCCAGAGCAGTGC 60.2 0.00
mm4__chr20 37.42 Mbp_chr20_37420185_R GTCTCGTGGGCTCGGGACACGGAGACAGATAGCGG 59.9 0.00
mm4__chr2 96.03 Mbp_chr2_96034495_F TCGTCGGCAGCGTCACGTTTGGAATGCTTGTGCC 59.9 0.00
mm4__chr2 96.03 Mbp_chr2_96034495_R GTCTCGTGGGCTCGGGTCTGACGAGGAAGCTGAGG 59.8 0.00
mm4__chr2 95.79 Mbp_chr2_95790062_F TCGTCGGCAGCGTCGTCTGACGAGGAAGCTGAGG 59.8 0.00
mm4__chr2 95.79 Mbp_chr2_95790062_R GTCTCGTGGGCTCGGACGTTTGGAATGCTTGTGCC 59.9 0.00
mm4__chr2 70.79 Mbp_chr2_70790686_F TCGTCGGCAGCGTCCTGAGGCCGGGCTTTCAC 60.7 0.00
mm4__chr2 70.79 Mbp_chr2_70790686_R GTCTCGTGGGCTCGGGGAGGCCGGGATTGGAATAC 60.2 0.00
mm4__chr2 68.64 Mbp_chr2_68636162_F TCGTCGGCAGCGTCGAGGGCAAAGTGAGGACAGG 60.3 0.00
mm4__chr2 68.64 Mbp_chr2_68636162_R GTCTCGTGGGCTCGGACCGGTGGATTTCAGAGCTC 59.7 0.00
mm4__chr2 241.95 Mbp_chr2_241953840_F TCGTCGGCAGCGTCCAAAGGCCTGGAGCAGATGT 60.3 0.00
mm4__chr2 241.95 Mbp_chr2_241953840_R GTCTCGTGGGCTCGGAGCGGAGACCTCAGGACAG 60.6 0.00
mm4__chr2 238.91 Mbp_chr2_238912654_F TCGTCGGCAGCGTCGCACACTCTGGTAGCCACAT 60.0 0.00
mm4__chr2 238.91 Mbp_chr2_238912654_R GTCTCGTGGGCTCGGCGTAGCCTAAGGTGTCGGTG 60.1 0.00
mm4__chr2 225.32 Mbp_chr2_225318241_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 225.32 Mbp_chr2_225318241_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 124.54 Mbp_chr2_124536694_F TCGTCGGCAGCGTCGGAGACATGGGCTTGGGTAG 59.8 0.00
mm4__chr2 124.54 Mbp_chr2_124536694_R GTCTCGTGGGCTCGGCCCCGTAAATCCAACCTGCT 60.0 0.00
mm4__chr2 100.98 Mbp_chr2_100981373_F TCGTCGGCAGCGTCTGCCTCAAGATATGGGAGTCA 58.2 0.00
mm4__chr2 100.98 Mbp_chr2_100981373_R GTCTCGTGGGCTCGGCTCCGCTTCCAGGAAACACT 59.9 0.00
mm4__chr19 56.03 Mbp_chr19_56032098_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 56.03 Mbp_chr19_56032098_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 52.74 Mbp_chr19_52735158_F TCGTCGGCAGCGTCGCGGAGAGACCTTGCCTTTT 60.6 0.00
mm4__chr19 52.74 Mbp_chr19_52735158_R GTCTCGTGGGCTCGGATGCCTCAGACTTTCTGGCG 60.3 0.00
mm4__chr19 50.46 Mbp_chr19_50462029_F TCGTCGGCAGCGTCGACACCTCTCATAGACCGCG 59.9 0.00
mm4__chr19 50.46 Mbp_chr19_50462029_R GTCTCGTGGGCTCGGGGAAGATTCCCCACAAGGCA 59.9 0.00
mm4__chr19 31.39 Mbp_chr19_31389214_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 31.39 Mbp_chr19_31389214_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 15.21 Mbp_chr19_15213827_F TCGTCGGCAGCGTCACAGTTCCCAACATGGTGGG 60.1 0.00
mm4__chr19 15.21 Mbp_chr19_15213827_R GTCTCGTGGGCTCGGGGAGGAGAAATGCAGTCCCC 60.1 0.00
mm4__chr19 13.80 Mbp_chr19_13795620_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 13.80 Mbp_chr19_13795620_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 1.09 Mbp_chr19_1092186_F TCGTCGGCAGCGTCCTCATTCACCCGGAGACAGG 59.8 0.00
mm4__chr19 1.09 Mbp_chr19_1092186_R GTCTCGTGGGCTCGGGGAGAGGTTGGTGCTGAGTC 60.0 0.00
mm4__chr17 8.00 Mbp_chr17_8002622_F TCGTCGGCAGCGTCGGGGCTCTCATCCTCCACTA 60.1 0.00
mm4__chr17 8.00 Mbp_chr17_8002622_R GTCTCGTGGGCTCGGCGCCCACAGGTCTTCCTTG 60.6 0.00
mm4__chr17 64.03 Mbp_chr17_64034451_F Primer3: not found at this Tm N.d. 0.00
mm4__chr17 64.03 Mbp_chr17_64034451_R Primer3: not found at this Tm N.d. 0.00
mm4__chr17 47.53 Mbp_chr17_47531868_F TCGTCGGCAGCGTCACACCTGTGTGGGGCTTTC 60.1 0.00
mm4__chr17 47.53 Mbp_chr17_47531868_R GTCTCGTGGGCTCGGGAGTCAGGTGGTTGAGGCAG 60.3 0.00
mm4__chr17 40.02 Mbp_chr17_40021545_F TCGTCGGCAGCGTCCTTTAGGCAGGAAGGTGGGG 60.0 0.00
mm4__chr17 40.02 Mbp_chr17_40021545_R GTCTCGTGGGCTCGGGCCATTGACTCACCAGCTCT 60.0 0.00
mm4__chr17 38.26 Mbp_chr17_38256549_F TCGTCGGCAGCGTCTGAGTCAGGTGGTTAGGCAG 59.0 0.00
mm4__chr17 38.26 Mbp_chr17_38256549_R GTCTCGTGGGCTCGGACACCTGTGTGGGGCTTTC 60.1 0.00
mm4__chr17 36.71 Mbp_chr17_36706305_F TCGTCGGCAGCGTCCCTACGTGTGTGCTCCAAGT 59.9 0.00
mm4__chr17 36.71 Mbp_chr17_36706305_R GTCTCGTGGGCTCGGAGAAAAGACCCACCTGTGCT 59.1 0.00
mm4__chr16 88.94 Mbp_chr16_88939826_F TCGTCGGCAGCGTCCTTCCTGCCTCCTCCCCT 59.9 0.00
mm4__chr16 88.94 Mbp_chr16_88939826_R GTCTCGTGGGCTCGGTTCTTGAGGCTGGGACTCCA 60.4 0.00
mm4__chr16 73.03 Mbp_chr16_73033611_F TCGTCGGCAGCGTCAACTTTTCACCCGGGGTCAG 60.1 0.00
mm4__chr16 73.03 Mbp_chr16_73033611_R GTCTCGTGGGCTCGGTCCTTTAACAGGCTGGCCTC 59.6 0.00
mm4__chr16 67.29 Mbp_chr16_67291570_F TCGTCGGCAGCGTCAGAGACAGTGTGAGGAGGGG 60.2 0.00
mm4__chr16 67.29 Mbp_chr16_67291570_R GTCTCGTGGGCTCGGCCAAGGTGAGGCTCTGGAG 59.3 0.00
mm4__chr16 544.23 Kbp_chr16_544225_F TCGTCGGCAGCGTCTCAGAGCACTGAGCAGAGCC 61.8 0.00
mm4__chr16 544.23 Kbp_chr16_544225_R GTCTCGTGGGCTCGGCTGTGCCCAGACAGCTCTC 60.0 0.00
mm4__chr16 2.93 Mbp_chr16_2932576_F TCGTCGGCAGCGTCTAACTGGGACATGGGCTTGG 59.6 0.00
mm4__chr16 2.93 Mbp_chr16_2932576_R GTCTCGTGGGCTCGGAAGTCTCCCTGTTCCCTGCA 60.7 0.00
mm4__chr15 45.15 Mbp_chr15_45147753_F TCGTCGGCAGCGTCATAGGAGAGGCTGGACAGGG 60.1 0.00
mm4__chr15 45.15 Mbp_chr15_45147753_R GTCTCGTGGGCTCGGCTTCTGGACACAGCAAGGGT 59.8 0.00
mm4__chr15 31.78 Mbp_chr15_31778052_F TCGTCGGCAGCGTCAGGACAGCAGGATCTAGGGG 60.1 0.00
mm4__chr15 31.78 Mbp_chr15_31778052_R GTCTCGTGGGCTCGGTCTCTGCACATTCGGCCATT 60.0 0.00
mm4__chr14 105.45 Mbp_chr14_105450197_F Primer3: not found at this Tm N.d. 0.00
mm4__chr14 105.45 Mbp_chr14_105450197_R Primer3: not found at this Tm N.d. 0.00
mm4__chr14 104.59 Mbp_chr14_104590179_F Primer3: not found at this Tm N.d. 0.00
mm4__chr14 104.59 Mbp_chr14_104590179_R Primer3: not found at this Tm N.d. 0.00
mm4__chr14 101.15 Mbp_chr14_101151542_F TCGTCGGCAGCGTCGTGGGGCAGAGTGAGTCATG 60.3 0.00
mm4__chr14 101.15 Mbp_chr14_101151542_R GTCTCGTGGGCTCGGGAAGCAAGCCCTGGAGTGTT 60.5 0.00
mm4__chr14 100.65 Mbp_chr14_100654771_F TCGTCGGCAGCGTCGATGGGATTCCTGTGTCGGG 60.1 0.00
mm4__chr14 100.65 Mbp_chr14_100654771_R GTCTCGTGGGCTCGGGTGGTCCTCGGGTCCTCA 60.2 0.00
mm4__chr12 68.54 Mbp_chr12_68538969_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 68.54 Mbp_chr12_68538969_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 124.56 Mbp_chr12_124564356_F TCGTCGGCAGCGTCCCACCTAGGGACACAGACCT 60.2 0.00
mm4__chr12 124.56 Mbp_chr12_124564356_R GTCTCGTGGGCTCGGGTGGGAGCTGGTAACTCCTG 59.7 0.00
mm4__chr12 124.45 Mbp_chr12_124453547_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 124.45 Mbp_chr12_124453547_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 8.08 Mbp_chr11_8081443_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 8.08 Mbp_chr11_8081443_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 64.35 Mbp_chr11_64348410_F TCGTCGGCAGCGTCGTCTCAGGTTGTCCACTGGG 59.9 0.00
mm4__chr11 64.35 Mbp_chr11_64348410_R GTCTCGTGGGCTCGGAGTACTGGGGTAGGAGGCAC 60.3 0.00
mm4__chr11 28.50 Mbp_chr11_28496369_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 28.50 Mbp_chr11_28496369_R Primer3: not found at this Tm N.d. 0.00
mm4__chr10 97.81 Mbp_chr10_97806153_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 97.81 Mbp_chr10_97806153_R Primer3: not found at this Tm N.d. 0.00
mm4__chr10 80.10 Mbp_chr10_80103827_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 80.10 Mbp_chr10_80103827_R Primer3: not found at this Tm N.d. 0.00
mm4__chr10 71.72 Mbp_chr10_71715200_F TCGTCGGCAGCGTCCTGGAAATGCCCTGCCTCTT 60.3 0.00
mm4__chr10 71.72 Mbp_chr10_71715200_R GTCTCGTGGGCTCGGCTTGGTGGCCCCTCTTCAG 60.0 0.00
mm4__chr10 70.51 Mbp_chr10_70512759_F TCGTCGGCAGCGTCCTGGGAGCACTGTCTCCTTG 60.0 0.00
mm4__chr10 70.51 Mbp_chr10_70512759_R GTCTCGTGGGCTCGGCAGGCTGGACTTCCACACTG 60.6 0.00
mm4__chr10 49.62 Mbp_chr10_49616703_F TCGTCGGCAGCGTCCTTTCCCCACCTACATGCCC 60.3 0.00
mm4__chr10 49.62 Mbp_chr10_49616703_R GTCTCGTGGGCTCGGCTGGGATCCTCAGCAAGCTG 60.4 0.00
mm4__chr1 3.77 Mbp_chr1_3772576_F TCGTCGGCAGCGTCCTAGCCCTTCCCCGCATG 59.8 0.00
mm4__chr1 3.77 Mbp_chr1_3772576_R GTCTCGTGGGCTCGGGGAAGGTCTGGGGCCAGTA 60.6 0.00
mm4__chr1 22.37 Mbp_chr1_22374480_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 22.37 Mbp_chr1_22374480_R Primer3: not found at this Tm N.d. 0.00
mm3__chr6 36.75 Mbp_chr6_36747127_F Primer3: not found at this Tm N.d. 0.00
mm3__chr6 36.75 Mbp_chr6_36747127_R Primer3: not found at this Tm N.d. 0.00
mm3__chr19 13.16 Mbp_chr19_13156172_F Primer3: not found at this Tm N.d. 0.00
mm3__chr19 13.16 Mbp_chr19_13156172_R Primer3: not found at this Tm N.d. 0.00
mm3__chr17 28.52 Mbp_chr17_28524632_F TCGTCGGCAGCGTCGAGCAAGTCCAGGGCCAC 60.3 0.00
mm3__chr17 28.52 Mbp_chr17_28524632_R GTCTCGTGGGCTCGGCCTCAAAGCCAAAGCCAGGA 60.5 0.00
mm3__chr15 90.20 Mbp_chr15_90201080_F TCGTCGGCAGCGTCAAACCAACGCCACTAGCAGA 59.8 0.00
mm3__chr15 90.20 Mbp_chr15_90201080_R GTCTCGTGGGCTCGGCACTGCTCAGACCCTCCCA 60.9 0.00
mm3__chr1 10.77 Mbp_chr1_10767437_F TCGTCGGCAGCGTCTTTTCCGCCTTCCCAGATCC 60.0 0.00
mm3__chr1 10.77 Mbp_chr1_10767437_R GTCTCGTGGGCTCGGTGCAATAGGGGAAGCCTGTG 60.0 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529699 TGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT
AGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG
mm4__chr7 10.38 Mbp_chr7_10379293 GTCCAAGAAGTGATCATTGCCCAGAAGAAAGAAACTGTCTACGAAGCATTTCTTTCCCCAGCCATGGTCACTTCTGGCAG
TGCTGCTATATCACTTCTATTCATCTTTCCCCCAAAAAACCCCACATGCATTCCTGT
mm4__chr16 75.22 Mbp_chr16_75219271 CAGGAGAGGGTGCATTCTGGGGCTTTCTGAGCCCACACAACCTGCTCCAGGAGTGGGAAGGACGATGCTGGTCTCTGCCA
TGGTCTGAGGCTCCAGAAGTGGCTGGGGTGGGGGTGCCTTGTGCAGGGTAAGGTGT
mm4__chr9 135.61 Mbp_chr9_135612564 GATGGAGGCCAGAAGCTGTCGCGCTGCGCTGAGCCCTGCCCAGCCATGGCCACTTCTGGAACCTGCATGCGGAGCTGAGC
CAGTGCAGGGTGGGGCCTCCCCAGGGCCCCAGGGGGTGTAGGCAGGATGCAGGATG
mm4__chr7 98.22 Mbp_chr7_98218122 AAGCAGTGAGGGTCAAAGGGGAAGACCTTCCCGGCCCCTCCTGCCCGTGCGGCCCGGCAGCCGGTGGCCAGGCCCCGCTC
TAACCACTTCGGGGTTTGGGAAGCTGCTGGGG
mm3__chr4 148.50 Mbp_chr4_148504571 AGGGCTACATGATGAGGGGAACCAGAAGTGGCCAGGCCGGAGGAAGGAGCCACACTGTTGGCTAAATCTGAGAGGGAGGT
AAGCCAGTGGTCTG
mm4__chr12 54.47 Mbp_chr12_54473591 CACAGTCACCTTCCTCCCACGCAAGCCCCAGCAACGGCCACTTCCGGCGCCTAACGTTGGGAGGTGGGGCGAGGGGCCGG
CACTTCCTGTGACTGGGAGTCTCGGTTCTGGCTGAG
mm3__chr7 47.12 Mbp_chr7_47122454 AGGCAGGATTAACACAGGACCGACCTCGCCATGGCCACTTCGGGCCTTCGTGACCCCAGCTGTGAAGGCCAGAGGACAGG
AGAAAAAAAATGCAATGGGGACCAGTAGAGCCAAAATAAGGCTTGTGTTTCTGGGCT
mm4__chr7 131.69 Mbp_chr7_131687005 TGAGGACCTAGCTCAGCTCAAAGCAGTCAGGGAAGCTTCCAGTGGGGAGGTGCTGTTTGCAGAGTCTATAAAAGACCCTG
AAGTGGCCAGGCTGGGTGGTGCAGGGCTGGGGGTGGTGTCCAGCAAAGAGA
mm3__chr3 149.75 Mbp_chr3_149752477 TGTCCTACGATTTCACCCGGCAGCGCCGACCCCGCCTTCGCCACTTCCGGCGCCCGTGTCCCGGCGCCTAGAGGCTCCGG
CGTCGCAGCGTCCGGAGCTTTGGGTTCCGGCCTACGAACTAACGCGTTACACTCGGACG
mm3__chr7 157.43 Mbp_chr7_157428889 CCTCTGGAGGGACCTGGATTGCCGGGGCCTGAAGTGGCCACAGCTGGGGCGGGGGGCCAGTTTCCCTGGGTGCTCTCTGC
ACCTGGGAGGGACATCCCAGGACATCCCCCAGTCTCACTGGGAAATCTGCCCAC
mm4__chr19 4.72 Mbp_chr19_4723761 CTCCATGCCCGGGACTGCGACCCCGGAAGTGGCGGGAGCGGGGGACGACAGCCGCGGCGGACACAGGGGACCCGCCGGCT
CAGGCACCTTTGACCCGGAAGTT
mm4__chr16 13.42 Mbp_chr16_13424353 TAATGACCTTGTGGCCAGCCCCTCTCATTTGCTACTCCAATGTTGCCCACTTCTTGGCCACTTCTGGCCTTATCATCACA
TTCCAGGCTTCTCTGCCTGTCTCTTCTGCTCC
mm4__chr19 18.93 Mbp_chr19_18928918 GCCGAATGGGTGCAGCTATAAGCTTCCCTGGCCACCCACCTGGCCACATCTGGGGTTTGTGGCCAGAGTGAAGGGGCCAT
GAAAGTCC
mm4__chr20 34.87 Mbp_chr20_34872848 CTGGCTGGCCTCGTTCTCCGCCGCCATCCTCGCCCGCGCCCCCCAGCAGCGCAGCGCCTGCCGGAAGTGGCTGCGGCGGG
GGAGTGGGAGGGCGGAGGGCGGGGCCTGATCTTCGCGACAGCC
mm4__chr5 179.19 Mbp_chr5_179191338 GGGCTCAGTGAGGAGTTTCCTTTCAAAGAGGGGCCCTAAGTGGCCAGGGCTCGTGCCACCCCCGGCCCTGGCATCCCCCC
ACACGCCCGTCTCAGAGGCTGTGTG
mm4__chr17 27.62 Mbp_chr17_27617394 AACTTCCCAAGCTGAACCGGCGGCTCTCCCACCCTGGACACTTCTGGAAGTCAGCTGAGTAAGTGCCTCTTCCATGAGCC
AGACTGCCAGCCAGGCCCTCGCAGCCTCACCTGGGGTCTTAGAGGG
mm4__chr7 2.01 Mbp_chr7_2014866 GAGTTCAGCATCCCCAGACCCAGTAAATGCCAGCAGCCCTGGCTTTGCCTCGGCACCCGCTCTGACCACGTCAGGCCTTC
ACTACAGCGGGTGTGGGCTCCACCTCGTACCGGCAGTATGGCTATGGGGTC
mm4__chrX 130.06 Mbp_chrX_130055027 CTGACAAGACAGAGCCCAGGCCAGAAGTGGCAAAGGCTGGAGCTGGGGTAGTGGAATAGTGAGGAGGGAAAACCCTGTGA
ACTGGGACC
mm4__chr18 49.02 Mbp_chr18_49021662 CCCATGACCTCACCTCTCCTGGCTCCAGGGCTCAGCCCTGCCCACTTCTGGAGAATTCCATGACTCAGGTAACAGAGGAG
GGGCCTA
mm4__chr11 110.71 Mbp_chr11_110711736 CTGACTTTGGGGCTGACACCGCCAAAGGGCAGTCAGTGCTCGCCCAGGCCACTTCGGGAGGCCCAGGGGCACGGCGAGGG
GTCGGGGAAAGGCGATCGCCCACTACAGCCCGCGCGCCTAGACTGAGGGAGGAG
mm4__chr17 74.37 Mbp_chr17_74368932 GCTGCTCCCAGAGGATGCCCCACCCTGTGCACGGCTGCCTGCCATCCTGCCCCTGGGGGTAGCCTCCACCTCCCCCACCA
TGGCCACTGCAGGACTGACCCTGTGCACCTGGTCCCCTGAGCCTCTTGGGATCATAGCCC
mm4__chr2 232.88 Mbp_chr2_232878385 AACTGAAGGTGTGAAGGGCCCAGGGCCTGTTTCTGGGCAGTGCAGGAAGTCCCAGCCCCATGCCTGTGGTGAGATCCCCT
GTAGGGCCCCCCCCACCATGGACACTTCGGGGCCTCTACGGTCTTCCAAAGCTGTGT
mm4__chr13 79.62 Mbp_chr13_79622838 GGGTAGCTGAACACCAGAGGACACCTGGAGTGGTCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNTTGTGGTTTACTCTTATGTTCCGTGATGAAGAAGCT
mm4__chr14 104.28 Mbp_chr14_104278635 CCTGGTGGCTTGGGTAATGTGGCAGCCCCCTCCCCAGGAGTCCCTGCACTGGCCACTCCAGGCACCAGGATTCTTTTTGC
CTTAATGAATTCCCCTCAAGTCTCCGGT
mm4__chr2 240.97 Mbp_chr2_240972340 AATATGGGGAGCCAGCAGTGGAGTTCTGGCCCTCCCGGAGCTGTTCTGTTCCTGAAGTGGGCGGGGTGGGAGCGGCGCTC
TCCGGTGCACGGTCACGGTGCGGTCCTCCACCTCCCCAGCTGCTTGCCTCACAGGGATG
mm4__chr10 88.90 Mbp_chr10_88897158 GGGACACAAAAGCCAAGAGGATTTTGCTATGGTTATGTAGAGAATAAACTGTTACAAGCTCAGAGCTGTGAAATACCAAT
TTGGAGAGGATACTTACCCCCTCCCAGGCCACTTATGGATGAGACAGCATTTTGGCCAC
mm4__chr11 68.97 Mbp_chr11_68967396 TGCCTAGAGAACAGGCTGGAGAAGTACAGGAGCTACTCAAGGGCCAGAAGTGGCCAGGGCGCCTTCTCTTGTTGGTTTAC
TTGTGTCGAGCTTCAGTTCTCTGGGAGTGTAGGTGTCAGTGGGC
mm4__chr15 74.37 Mbp_chr15_74369590 ACTTGTGGTCCTCTTGGCAGCCACCCCAACTTCCGTTCAGGCCTCCGCACCTCCCCCAGTGTTGTGGGGCACTGACCGCC
TCCCCAGAAGTGGCCCGGGCTGGAGGTTATTGCCTCATCCTCTCCAATGGCCATGGTCAC
mm4__chr11 69.48 Mbp_chr11_69475132 AGACAGAAGCCTGAACCTGCGTGTCGGGTTGGCACAGCCAGAGGTGGCCAGGGAGGGGTAGGTCAAGGAGGCCTTCCTTG
CCTCCTTGAGG
mm4__chr19 4.36 Mbp_chr19_4361924 CTCAGACCTGCTGTGACCTGGAGCGTGTGGGTGGGCATGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNTCTGGGGTCCGGCCTCTACTGCCACCCAGGAGGGAGGATGGGAGAA
mm4__chr1 22.25 Mbp_chr1_22248671 CATGAGAGTGGAAGCCCTGGGGGGAGAGGGAGTATGGGAGGGCCAGCAGTGGCCATGACGGGTGCAGGTGGCCCCCCAGG
GCTGACCCGATCTGATCCTG
mm4__chr2 240.63 Mbp_chr2_240629867 TTTGTGTTTGTGGGTGTGGGGTCGGGGCTCACCTCCTCCCACATCCCTGCCCAGAGGTGGGCAGAGTGGGGGCAGTGCCT
TGCTCCCCCTGCTCACTCTCTGCTGAC
mm4__chr9 133.47 Mbp_chr9_133472583 GGACCCTCTAGGCTGATGGACTCACTCTGGGTTGCCTGCCCTCCCCCAACCCCCCTGCCCTGACCACTGCAGGGACAGCT
TAGGCGCTGGCCTAGGCTGCCCTAGCCAGCTTCTCCCTCATGGGAACTTACTCTGGGCCT
mm4__chr8 142.91 Mbp_chr8_142914987 GATGCACTGCTGAGCACAAGGCAGCCCCAGGCCTCCTGGCTGCCTCCCCACACTCCCTTCAGTCCTCCATCCCCATCCCT
GGCCACTCCAGGGTCTCTGGGGCTGGACCTTCCCGCATGGCCCACACCTTCTAT
mm4__chr2 2.56 Mbp_chr2_2560320 GCTTGTGCTGCATGTAGCTGAGCTCCTCTGCTCCCTACTGCCCTCCCCATTGCCACTTCGGGGCTGGCGCAGGGTCCGGT
TCCTCCTCTGTTACCCTCCCTGG
mm4__chr11 67.67 Mbp_chr11_67665921 CTCCAGGCACCTGACTCTTGCCCGCTTTCTTCATAGAAAGTCCTGGCCCGCCTCACCCTTCTCCTGGCCGGTCTGGCTCA
CCCACTGGGACCTGCCTCCAGCCTGGCCACTCCTGGATCCAGTTGCTGCAATGTGC
mm4__chr16 1.37 Mbp_chr16_1366532 CAGAGACAGGGTCAGGGAGACGCCCCAGCCAGGGCCACCTCAGGGAGAAGGCAGCTGCGGGGTCAGGGGGTATGCTCTGT
CTTAAGAGCCCTGGGC
mm4__chr13 40.64 Mbp_chr13_40635786 CAGGAAAGGCCTCTGGATGGTCAGGCACACCACTGAGGAGAGTGAGTCCTCTCCCCTCCACTGAGGGCCTGCCCTGCCCA
CTTCTGGGGACGTGACAGAGGTCCCAGCCATCAGGATGAGCTGTACTCTACCTGCTGCC
mm4__chr10 77.04 Mbp_chr10_77038917 GTGAGGAATCTGAGGCACTGTCATGCCTCACGGCAGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNAGTGGCCATGGCGGGTGGCTTTGATCCATCCTGCTGAGCCAGTGGAAAGA
mm4__chr10 111.12 Mbp_chr10_111115738 ACAGCAATGAGGAGGTGCCGGGGGTTCAGTGGGGCTCTCTGACCCCTCCCAGGCCACCTCAGGGGGTGCCTGAGTGCCAT
CTCAGAAGGCTCTCTGGGTGA
mm4__chr19 19.55 Mbp_chr19_19546006 ACTTGGTGACATCCGCAGGGAGATGAGCGAGGCGGCGCAGGCACAGGCCCGGGCCTCAGGTCCCCTCCGCACCCGCCGGG
GTAGGGTCCGGCAGTGACCTGGGCAGGGGCCTCGCTTTCCCACCTCCCTCCAGACTCCTT
mm4__chr16 74.30 Mbp_chr16_74297172 GGCTGGTGACCCTTACTTGTTCACGAGTCCAGACCAGCGTCGGCTCACGATGCCGGAGTCATCCAGCCTCCCGCCCCTGC
CCCAGCCGCCGCCACTTCGGGGCCTTGGCTGCCTGTTCCGTTTA
mm4__chrX 153.08 Mbp_chrX_153075591 GGGGCAGACATCTTCAGGTGCCCCCACCCAGGCCACAGCTGGCCCCGATCCCGATGTGGGGTGAGGGACCGGCCCTGGCC
CGGCCTCCGCCTGTCTCCAGGGCCCTGCTGCCTGAGAGGAGGAGAGGAGG
mm3__chr8 71.82 Mbp_chr8_71819187 GGGCTCTGGCAAGGAATGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNCAGTTCCAAGCCTGGGCCTGGCAGTGTGAGTGGAG
mm4__chr22 39.59 Mbp_chr22_39594601 AGGAGGAGCTGAGAAGAGGCCACGAGTAGGCAGCATCCCAAAGTGGGCAGGGCTGGGGATGGGAGGGAGGCTGTTGCCAT
GGAAACCAGGTCACCTCTCCCACTCCTCT
mm4__chr11 455.89 Kbp_chr11_455892 ACTGGAAGGAAGGCTCAGGAACGCAGGCTGTGTCTTCTCCCGGGTGTGGCTGGGGCGGGGGCTCAGGGAATGGCTGTGAT
GGCCCAGTGTCCAGGAGGCTCCGGGCCCCACCCCCCGCCTTGCTGCATGACCTTCACAGT
mm4__chr20 37.41 Mbp_chr20_37406610 GTGGATGTGGGTGGGAACAGCATGTGTGCCCGTGGACCCGTGTGAGCTTGGGCTCGCACCCCCAGCGCTGGCCACTCCCG
GCGGGACCCACGTGTGCCCGGGGGCGCAGGGCCACGTGCGAGCGCAACCGGGACAAAG
mm4__chr15 31.81 Mbp_chr15_31813006 CGCACACACACACAGGTATGAGGTCATGCCACATCATCTATTCTCCCTCAGGATGGATTCTCAAGGCCAGGCCCTTCCCT
GGCCACTTTCGGAACCCAACCCAAAACACAGAGAAGGTAGAAGCAAGACACCTGATGCG
mm4__chr9 129.75 Mbp_chr9_129748618 GTCCCCTGTGTGCAGAAGAAGTTCTGAAAGGGCAGGCCATGGCCAGGTGTGGCCAGGCCAGGGGCCCGAAGTACTTGCCT
GAGGCAGCGTTCCACGTCGGGGTCGCTCCTGCAATACTG
mm3__chr16 10.84 Mbp_chr16_10835453 ATAACAGACCAGCCAGCCACACCGCTGCCCTGGCCACTTCTAGAGGTCCAAGTCTCTTGAGATCCCCACTGCCTTGTGGA
GAGGGCCATGAATCCTG
mm3__chr3 14.52 Mbp_chr3_14517814 TACATCGGCTTTGGTCCTGGCCAGCAGGTGTGGCCCAGGTGGGCATCCTGCAGTGGCCGGGCCGGGGGCTGTGGCAGGAG
GGCACGCAGGGGTCCCAGCGGTGCAGCTGCTCACTCCTCTGCACTT
mm4__chr10 129.74 Mbp_chr10_129741738 AGCTTTCTCATGCATCCCGTTTCCCAGAGGTGGCCAGTGCCTGGGCCCTGAGTCGGTTGAGACTCATCCAGAGCAGAGCT
GCAGAAGCTGGGCTGGCTCCAGACAGAGATGACCCAAAGCCTGTGTCAGCATTTGGGTGC
mm4__chr16 88.73 Mbp_chr16_88726525 TCCATCATCTCCTTGGCTGCAAGGCAGGCACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCTTCCCCACGCCC
TCCCCCGCCACCGTCCTGGCCACTCACGGTCATAGTAGCCCTTGACGGTGCATA
mm4__chr12 109.83 Mbp_chr12_109828322 TCCTGTGCGCCTGGTGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGAAACCAATGCTCAGG
mm4__chr15 75.29 Mbp_chr15_75294388 AGTGAGCCTGGACAACAACGTGGAGCCTGCACCAGGAGTGGCCAGGGAGGGTTCTCCCCATAACAACCCCACTGTACAGC
AGATCGTGCAGCTGTCTCCTGTCAT
mm4__chr7 31.07 Mbp_chr7_31070366 CCCAGAAACCTTCCCTGCATCTAAGCAGGGTTGGTCGGTGTGGTCCTCCATGGGGGTCCCCACACTCTAGTGGAGGAGGA
AGAGGAGGCCTGTGGTGGCCATGGCAGGGGCAGCAGAGAGCAGACTTGGAGG
mm4__chr7 35.98 Mbp_chr7_35982717 CTTCCGAGGGCCCAGTAAAGACTCAAACCCTCAGGCTTCCGTCCCCACGTCCTAAGTGGCACTGGACCCCAGCCTGGGCT
CTACGTGTCCCCCGCCCGCGCTGGCCACCTCCGGGTGGACCCTAGGATTCCGTAGCCG
mm4__chr1 2.92 Mbp_chr1_2923105 GGGCCCTGGTTACCTCTAACCCACAGTCTGTCTCTAGGGACCTTGTAAGCCTCCCCCAACCCTGCCCAGGCCCTGGTCAC
CTCTGGCCCACGGTCTGTCTCTAGGGACC
mm4__chr16 57.10 Mbp_chr16_57103553 CAGGACATCCCACTGTGGTCCCAGCTCCTCCTCTCCACCAGCCTCTGGAATGGGGGCTGCCTCCCTGGCCACCTCAGGCC
TGCCCTTCACCATCCAGCAGCTCTTCTCCCCTTC
mm4__chr7 165.54 Kbp_chr7_165545 TGATGTCTCGTGATCATTTCTCTTAGTATACATTAAAAGTTAATTATTGTCAGAAAAAGTTATCTGAAACCTGGAACTAA
GTCCCTAAAGTGGGCAGGGCTGGGGGGAAGGTTCCCGAGACCTGGGCTGGTTC
mm3__chr14 91.27 Mbp_chr14_91272976 GGTGAGAAGTGCAGGAGCTGGGAGTTGCTGCCACTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGCAATGGCGGGGGC
TGTGGCAGACTGATGCAGG
mm3__chr11 557.07 Kbp_chr11_557071 ACTCCACGGTCTTGTGATGCCGCTCGGAATGCTTTCCAGAGGACTCTGGGCTCCCTGTGTCCAGCTGTGGCCAGGGCCGG
GGCTGCCTGTGGACCACGCTCTGCTTGAT
mm4__chr8 142.88 Mbp_chr8_142876964 CTCCCCACACTCCCTTCAGTCCCCCATCCCCGTCCCTGGCCACTCCAGGGTCTCTGAGGCTGGATCTTCCCA
mm4__chr7 44.23 Mbp_chr7_44229414 AGCAGGACCCAGGCTACTTACATGGGGCTGGCAGGGGGCTAAAGGGAGCCGGAGATGGGCAGGGCAGGGGCCCCTCGGCT
TCTGGGGCTCCCGAGCCCCTCCTCACTGAGCTCAGGATGTCGG
mm4__chr17 74.92 Mbp_chr17_74923077 TGGTACTGGTCGTTCATGGCGCCCGAAGTGGACGGGGCGGGCGGGGGACACGGAGAAAGGCCCCCCGCAGGGGAGGGCGG
CGGTATTAGGGCTGAGGCATGAGGTTGGAGG
mm3__chr1 204.69 Mbp_chr1_204685017 CCCACTCTACCCAGCAACAGTTTGGGGGAGGTTCTGGCCTCTCCAGGCGAAGGCCCAGGCGCCCTCTCCCTGGCCGCTTC
TGGGCATTCTCCAGCCCTCCGTCCCTCTGCCCCTCGGCCGCCTCCCTCTCCCAAGTCTCC
mm2__chr10 85.79 Mbp_chr10_85787237 CAGTCCTGGAAGGGTTTGGTAAATGTGCCTGCCCCTGCCCTGGCCACTCCCAGGTATCCAGTTGGGCCTACTGGTGCTGA
CATCCTCATTGCCTGGATCTGAACACTCTGTCCTCAGGTCCATCTTGGCCATGCACTC
mm4__chr19 11.19 Mbp_chr19_11193115 CTACCACCCGGACTGTATCCACGCGGACCGGGCTGTCCGGCGGTGGCCAGGCCTGGGGCTGGGGGTCAGCCTGCCGGGCC
TGAGCTGCCTGCAGCTCCTGCAGCGCCTTCTTGAGCTGGGTCTCC
mm4__chr8 144.10 Mbp_chr8_144104974 GCTGCCTCCTGGGATTTGTAGTTCCACCGCGGCTTGGCTGGTGCCCGACGTGGCCCAGGCGGGCGCTGCCTGGTGACTGC
AGCGCCCCAGCGGGGCCGAGGGACCGCCCTCCTAGGCGTCTGGAGAAACTAGGCA
mm4__chr7 157.28 Mbp_chr7_157276311 CGTGCAGCAATGATGTGTGTGTGAGCACAGGCCCTGGCCACTGCGGGGCAGGCAGAGGGAGCCGGTTCTCCACCTGCCCA
GCTGGGACCCCAGTGTGGGACCTGTTTCCTGATCCG
mm4__chr7 2.48 Mbp_chr7_2477210 GGGAGGTGAGCAGAGAGAGTCTCAGGCTGTCGGGCAGCTTTGGGGCCTCCGAGGACGGGAACCGGCCCGAAGTGGACCTG
GAGGGGGCACAGAGCCCTAGGGCTGCTGCAGGTG
mm4__chr2 231.90 Mbp_chr2_231900688 GAGAGCACGGACTTCCCTTCTGCCGTCCACGCCCATTCTGACCGCCAGGCGGCGCCTTGGTGCCTGGTGCCAGAGGCCGC
AGGACCAGCAGTGGCCAAGGCCGGCGCTTACAGTCCCAGGGATCAGAGGGGACGGTAGA
mm4__chr3 23.71 Mbp_chr3_23705376 TAGAGGCAGGATGCTGGAGAGAACTCTTTCCTACCTGCACCCTCCCTGCCCACTCCTGGTCAGTTCAATGCCTGGGGAC
mm4__chr8 23.20 Mbp_chr8_23199935 GGTCCCCTCCACAACCTAGAAGAGAAGACGGTTCCCTTAGTGGCCAGGGAGGGGCCCATGCAGCACTCCTTCTTAGAGGG
CAGTGTTGGGCAGGGGTGGGCTGGGGGCTGGGACACTGGACAAAGA
mm4__chr1 72.42 Mbp_chr1_72419402 TTCTTAGCCAGCGTGGAGTGCAGGGCCACTGGCGCTTTGCCTGGCGTGGCCATGGCGGGGCCCAGTTGGTCCCGACTGCC
GGCCCATTCCTACCTTCAG
mm4__chr1 18.87 Mbp_chr1_18869287 ATCCGGCCTCAGTCAAATGGTTCACGCTCCAAGCCCTGGCCCTGGCCACAGCAGGGAAATGAAGGGGCTCCTTGGGCCAC
CGCAGACACAGGGACTTATTGTGGTGACCATGCAAAGAGGCCTCAGGTC
mm4__chr20 51.51 Mbp_chr20_51506885 AGCAGATGTCTCCTTTCCCATATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGTCACATGGCCAGCCAGG
mm4__chr2 112.63 Mbp_chr2_112626771 TGCTTAGGAGACGGGACAGATGGGCACCTAGCCCCCTCACTGCCCACTGCAGGGTGGGCATGGTGCCCACTTGCTGGTGG
CAGCAGCACCAGAGAAACAGGATGCACCAGGAGG
mm4__chr12 52.38 Mbp_chr12_52377352 CTCCTTGGAGCTTGCAGACACAACCCTGCAGTGGACAGGGCTGGGCCTCACACCACGAGCAGGGGCAGGAGTACGGGGAT
CATTGCAAAGCCCGCCTGTCCGCCTGCACCCCACCTCGTGGACTTGCATGCTGGAGT
mm4__chrX 71.12 Mbp_chrX_71118792 TCTTGAGCTACGAACACCGGCCCCTGAAGCGGCCGCGGCTGGGGCCTCCCGATGTTTACCCTCAGGACCCCAAACAGAAG
GAGG
mm4__chr2 32.95 Mbp_chr2_32947903 GGTCAGGGCCACTCGGAGCCCCGCGGTGGCCAGGGCGGTCGCGCGCGGTGGGTCGGCTTTCTCCTC
mm4__chr12 45.05 Mbp_chr12_45050718 CAGCTTTAGGGGAACTGGGCTAGCAGGCAGGGGCCGGAGCTGCGCGCCCGCGGTGGCCAGGGCGGTCGCCGGGTCGTGGG
GCGCGGGCGGCTGCAGCCTGGGCGTTGGGGCGCCCCCGACCCGCAGCAAATTCTCGATC
mm4__chr19 41.35 Mbp_chr19_41353690 GGCAACGGAAAAGTCTCAAAAGTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NCCGCAGTGGCGGGGGCGGCGGCGGCTCGTCTCAGACTCTGGGGCCTCAG
mm3__chr9 95.38 Mbp_chr9_95384073 TGCCAGTGAAGAAGGCAACACAGACTGAACTTTTTCCCCCTGTCCTGGCCACTTCTGATTGCTGAACTGAGTGCTCTTGA
CCAATCTGTGAAGCCAGATGTCCTGGATGCTTGTGAGT
mm4__chr4 143.70 Mbp_chr4_143700664 CGACTCAAGAGCAGGCAGGCGAGCGCCACAAGGAGCTGCCGGGGCGTCCCAGTCGGGTGCCGAGAAGCCCCCGCCATGGC
CACGGATGGCTCCTGGCGTTGGGATTCC
mm4__chr11 111.88 Mbp_chr11_111878890 TCTCCACGTGGGTTACATCCCCACGCCCCCACCCTTTCCACTTTGGGACGCGCCGGCCGCTGGGCGGGGTTTCGCAGAGG
GGCGGGCTCGCTACCTCGCTCGCGCAGGCACTGCAGATTCTCCCAGGCCAGTG
mm2__chr7 106.01 Mbp_chr7_106013485 CCACACCGTAGAACCACTGGAGACCAGAGGAGAGTGAGACAGTGAGGATGTGTAGGAAGCCCCCTCCCTGGCAACTTCAA
GGGACAAAAGCTGCATTGGAAGAAGGATGGGCATGAAAAGTGGGTCC
mm4__chr22 47.03 Mbp_chr22_47029869 TCTGTCTTGAGCTGTCAGCCCCAGACTGCTTCCCCCAGAGTGGCCTGGGCCGGGGCTGCTGCTCGTCCTGGGTCTCAACC
CTTCCCACCCTCTGCCCACCATTGGCAGTGAGCGGTTCTTGGTGTTCTGGCTGGTGAG
mm3__chr20 676.45 Kbp_chr20_676446 CCTCCAGTCCTCTCCCTCTCCCCCAGTTCTGAAGTGGCCAGGGTGGACGCTCAGCTTTCACTAGAGTAAGTCTCCCCTCC
CCCCTGCTCGGGCTCCCACTGGAGCCCCCTGTTGGGTTTGGGGTTCA
mm4__chr19 40.43 Mbp_chr19_40425623 CCTGGCGGGCTGTTCTCTGCGCCCGGCCTCCGCTCGCTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNGTGAGTCACGCAGCCGGAGCCAAGAAG
mm4__chr3 122.91 Mbp_chr3_122913135 GCTAGGCCTCAGCGACTGGGCGCCCGGCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCACCG
CTCCGGGGCTGGGAGAGAGGAAGGAGGG
mm4__chr18 12.88 Mbp_chr18_12883577 CTGCTCCCACAGGTCCTGCGCGGGCGCCAGCTGGACCCGGACGCCGCGGCCCGGCTCGCCCGCCCCCGCCCTCACCGCTC
CCGGGGACGCCAGCTGCCCACCCGGCCACAAAACACCCGGCCAGAGAAA
mm4__chr17 81.09 Mbp_chr17_81085955 ACCTGTGGGTGAATCCGTTGCCTCTGACGCCCCACATGCCTGGTTCCATGCCCCGGCTTTGGCCACTTGCGGTGAAGCTG
GTGCCTGACTCTGCTGCCGGGGAGTGCAGGAGCTCCTTCTCTGGCCTCTGCAGTTC
mm4__chr15 31.39 Mbp_chr15_31391608 GCCCCACACCCATTTCTGGGATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACGCTTCCGGGAGGTCTAGGCGGCTG
CCGTGGGCCGGTCCGAAACGGCTCTAGGTGCACT
mm4__chr15 94.23 Mbp_chr15_94231540 TCCTCCCCTTTAGGCGGGGCTCGCAAGGGCGGTGGGGGGGCCCGAGGAAGTCCGGGGGCCCTCGGTGATGCCCCCGCCCC
CGCCGCGTCAGGGTTGCTCCCTGCGCTTCCGAGTGGCGACTG
mm3__chr20 23.24 Mbp_chr20_23241765 GGTCTGAGGGAGGAAGAGGAGAGGCAGAGCCCAGGCAACCTGGGCCCCCTGCCTGGCCAGTTCTGGGATGCTGCCCTCAG
CAGAGCTGGGGGAGAACTGTCACACTTTCACTGCCCTTGC
mm4__chr19 7.91 Mbp_chr19_7912603 ACTGAGGACAGAGAGTGGGGGGTGCCCACCCACCCCCCCCGCCCCGGGCCTACCAAGCCCCCGCCCTTCCCACCCCGGGG
TCAGCCGGCCGTGTGCGTCCCCCGACAGACACTGTGAACGGAAGACAGCAGG
mm4__chr3 98.02 Mbp_chr3_98024574 GCCTATTGTGCTTCCCTTGGTCATAACCACATCATGAGAAGAGTATCCCCCGCCCTGAACACTTGCGGTATTCGTTATTC
ATCAGAAGGCTGTATCTGTTATACTGCCAGAGGATCTTTCTTCTCACCC
mm4__chr16 73.06 Mbp_chr16_73057985 GGTGGGGCAAAGTTGGGGGCCGGGAGCGGCGGGGGCGGGGGCACTCGGCACCGCGGACCCCCCCATACTCCCCCCACCGC
CGGCCGCCGCCTTTGTCTCCGAGCTGGTACC
mm4__chr9 134.37 Mbp_chr9_134370325 CAGTGTGACCTCGGGCAGGGCTGCGCTGAGCTTGGCAGGGCTCTCTGCCCTCGCCGTGTCCACTGCTGGGAGCACGTCAG
GCCTCCCTGCCCGGCTGGTTCCAAGGCCTTTCTGG
mm4__chr19 58.10 Mbp_chr19_58096609 AGAGTCCTGCAGCAAATCCCCTTCATCCTGGGCTGAGCAGAGCTCAAGCCTGGTTTGGCCCTCCGTGGGCCCAGCCCTGG
CCACCTGAGGAATATTCATCGCAGCTCTCCATCC
mm4__chr11 62.15 Mbp_chr11_62145889 ACCCAGACCATCTTGTTGCTTCATGCTAAGAAGGAGGTTTCCCAGAAGTCTCCAGGGCGGGGATGGTGACCAGAATCTGC
CTCT
mm4__chr15 31.39 Mbp_chr15_31392830 CCCCACACCCATTTCTGTGATTGCCCCCCANNNNNNNNNNNNNNNNNNNNNNNNNNGCGACGCTTCCGGGAGGTCTAGGC
GGCTGCCGTGAGCCGGTCCGAAACGGCTCTAGGTGCACT
mm4__chr11 69.00 Mbp_chr11_69004487 AACTTCCGTTGGCCACATTGGGCCACTGTGCACCACAGTGCCAGTCCCCAGGTGGCCAGGGCGGAGCCAGTGTCCACTTT
CGGGTGGGGGCTGGAGCCAAGAGGCTGTCCAGGAGC
mm4__chr6 53.53 Mbp_chr6_53529782 CCTGATCCCCAGACAGAAGCTGCTTTGGATCAACTGCCCTGCAGTAGCCAGAGCTGGGGCACTGTAGTGGGTGAGAATCA
TGGAGAATTTCTGCAGCGGT
mm3__chr3 50.37 Mbp_chr3_50371930 GACAACTCAAGCCAGTGGGAGTAGGGCACATCCCCCACCCCCGCCCTGGCCACTGGAGGTGGCTCTGATCCCTTTATCCC
CTTTGCTGGACTAGGCTGGAC
mm3__chr20 20.39 Mbp_chr20_20388362 AACCATGTCCATATCTGAGAAGTATCCCTGAATTATCCAGGGCGGGGGCATCTCCAGGGAGATTATCCAGGGAGTCCTGA
GGGCCACCTATGACGCCGAGTCATTAGTGAGTGGCAGAAACGCA
mm4__chr17 46.82 Mbp_chr17_46819903 GCCCTGGGTGGTATTGACATCATGGTCAGGTTTTGGGGACTCACGCCCGGTCACCTGGGTGCCTTTCCCTAGGCCCCCTC
CCAGGCCACCTGAGGCACCAGGACTCTGCGCAGCCCCGGTCTGGGGTGAAGGAAGCAC
mm4__chr18 23.45 Mbp_chr18_23453176 GCTGATGCTTGCTTCTCCGAGCCCGGCGGTCAGCAGCCGGAAGTAGCCCGCCCGGGGGCGGGGAAAGGTGCCTTAAGGAG
GAGCTAGGGGCGTGCGGCCAATTGCAAGCGACTGTCCTAGCGAA
mm4__chr3 13.93 Mbp_chr3_13933043 GGAGCCCTGAGTCCGGAGCGGAAGCGAAGCCGCGCGCTGCCCCGGCCGCCGCCCACGCGACTTCCGGCGGCGCCGAGGCA
CGCGCGCCATAAGTTGGAGCTCCCGGGGCGCCCGGGACGAGCTTCCCAGGATTC
mm4__chr22 44.33 Mbp_chr22_44331460 CCTTTCCCCGCCGCTCTCGGACCCCGGCCCAGGCGCGCCCCTCCCCCGCGCGGCCCCCGCCCTGCCCGCCCCGGGGCTCA
GCTCACCTCGTGCCCGCCGCCCGCTCGTTCCTCGCTTCGTAGC
mm4__chr2 171.52 Mbp_chr2_171515798 CAAAGGAAGAGGCTATGGACCAATACAAATGAAACCTTCCACTGCCCCAGCCCTGCCCACGTGGGGCAGGTGGACCCAGA
GCCTCTTGAATTGGAGAACTGGTAGAGGGCTGCTTCCACAGCCAGTATCCCCACAG
mm4__chr11 65.63 Mbp_chr11_65634071 GCCTCTGAACCTCCATGCTTTCTCCTCCAGTGGCCAGGGCCGGGACCCCGGCCTGACGCCTGCCCCTCCTCTCCCAGAGC
ATCATCTACTACGTGAGCCGCTCA
mm4__chr4 1.63 Mbp_chr4_1625853 CCAGGTGATCAGAAGGCCCCACGTCCCGGCCCACGTGGTCAGGGTCACGCCAAGTGGAGCCTTGGGGGAGACTGAAGCCA
CCGCCCTGGCCAGCACAGGTCATGTCTGGGCAGCCAGGAGCTGTCCAGAGACCCCAGAGG
mm4__chr16 26.55 Mbp_chr16_26547167 TGGCTTCATGGTTTGGCAGAATGAAAAGAAAATGCCAGGAGTATCCAGGGCAGGGGCTGGGCACTTAAATCCTATCCTCT
CCAGGCT
mm4__chr19 50.94 Mbp_chr19_50943407 AAAGTGCCCCAGGGAGATTGAGACGCAACCCCCGCCCTGGACAGTTTTGGAAATTGTTCCCAGGGTTCAACTAGAGAGAC
ACGGTCAGCCCAATGTGGGGGAAGCAGACCCTGAGTCCAG
mm4__chrX 119.61 Mbp_chrX_119605881 CACTTTCGTGGCTATAGCGCCACTGCTCCAGCACTGGCTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNCGACAGGAAGTCGCCCCCCAACAGCAGACGTCCTCCAG
mm4__chr9 134.40 Mbp_chr9_134404971 TGCCAAGGATCTGAAAGGGGAAATGCGATGGCCCCCACCCTGGCCACGCGTGGAGTCCTGAGGCTCCAGCGAAGCAACAA
TGG
mm4__chr13 113.08 Mbp_chr13_113080728 GGATCCAGTGTGGTGGGTGCAGGGTCACTCCCCGGACCACCACTGCCCACCTGCTGTGTGTGAGGGCGTGGGCAGGGGGC
AGCTCCGGCGATGCCAGACCCCCGCCCTGGCCACGAGGGGCCACACAGATACCCACCACG
mm4__chr17 80.47 Mbp_chr17_80469592 CACTGCTCAGGTGTCAGTGTCGCCGGCCAGTGGCAGCTTGTGGGCTGCTGTAGGCCGTGAGCCCCATGAGATGGAGGAGT
GGGTTTCAGGCCCCTGCCCTGGCCAGGCCTGGCTCCACACGTGAGGTCTCTGGAG
mm4__chr19 4.40 Mbp_chr19_4400236 AACGTCCCCACCTCGGTCCCCCCGGCCCCCTCCCGGGCCAGGTCGGGCCTGGCTCCCTCATCCGGGCAGCCCGGGGCCCG
GTACTCGGCTCCCGCCTGGGCCTGCGCTCACCTGGCTCGCCTTGTAGAACTG
mm4__chrX 143.63 Mbp_chrX_143634510 CCACCCGAGACCTGTACAACTTGCCCCGCCGCCCTGGCCGCTTGGGGTATGGTGGGGGGGGGGGCGGGCAGCGATGCCAA
GGACACCCGGATCCTTT
mm4__chr6 168.88 Mbp_chr6_168875240 AGCAGGGGTAGAGGATGGAGGGCCAGCAGCACAGCCATGGTCCCGAGAGTCACTCCTGAACTAGCCAGTGTGGGGGCCAG
CCCTTCTGCTCATGCAGCCAGTGGTCAGCTACAGGCACAGTC
mm4__chr12 49.06 Mbp_chr12_49060400 CCCTTCCCCTCTGGCCTCGGGAGCTGCCCCGCCCCCGGCCTGGCCGGCTCTGGTCGGTGGCACCCCCTCGACCCCCGGCT
GACTGTGATCGCAGGCGGCCTAGAGGTGCATGGCCCAAC
mm4__chr9 133.38 Mbp_chr9_133376656 GTCGCAACCCTGGAGCTACGGCCGGCGGTTCCGACCGAGGGCGGCGAGGGGCCCGCGCCCTGGCCAGTGTCGGCCTGCAG
CTCCTAGGTTGAACCCG
mm4__chr22 37.34 Mbp_chr22_37335179 GGGGCGTAAAGAAGGGTCATGGGCTCCGGTGGTCGCAGGTCCCACCGGCAACCGCCAGGGCGGGGGCAGCAGGAGCAGGA
ACCTGCTCGCCTC
mm4__chr8 28.27 Mbp_chr8_28267546 GGCCAAGGAGCTAATGGAGGAGACTCCCTGGGCTTGGAGATGCAAGGCCTGGAGGGGCCAGGGCAGGGCCTTCCATCTGG
AGAGCTGAGCAACCCTCCCTCTCCACAGGCTCCAACCGAAGAT
mm4__chr8 143.29 Mbp_chr8_143291348 GGCAACCGATGAGCTGAAGGCGCCGCGGCTGGGCCGTCCCGCTCTCCCGCCGCCGAGCGCGCTGTCCTCCCGCTGCCCAG
AGCGTCGCCGGAAGTGCCGAGGGGGCGGGCCAGGGGTGGAGACTGGCGTTTC
mm4__chr7 88.64 Mbp_chr7_88639743 TTGAGGCACTGGACTGCATCCTAACACCAGCTTGTCCAACCGACAGGCCCTCGCCCTTGCCTCTCCAGGATGTCTACAAA
ATTGGTGGTGTTGCTACTTTGCCTATTGGCGGAGTGGAGACTGGTGTTC
mm4__chr7 4.80 Mbp_chr7_4802889 GTCATGCTGGCTGGACCCCCTCCCCGGGCACCTCGGGGAATGCTGGCTGGACCCCCTCCCCGGGCACCTCGGGGCACTCT
GGCTGGGGGGCCCCCTCCCCGGGCACCTCGGGGAATGCTGGCTGGGTCCC
mm4__chr7 4.80 Mbp_chr7_4802739 GTACCTCGGGGCACGCTGGCTGGGTCCCCTCCCCAGGCACCTCGGGGCACGCTGGCTGGGCCCCCTCCCCGGGCACCTCG
GGGCACGCTGGCTGGGGGGCCCCCTCCCCAGACACCTCGGGTCATGC
mm4__chr7 4.80 Mbp_chr7_4802442 GTACCTCGGGGCACGCTGGCTGGGCCCCCTCCCCGGGCACCTCGGGGCACGCTGGCTGGGCCCCCTCCCCGGGCACCTCG
GGGCACGCTGGCTGGGGGGCCCCCTCCCCAGGTACCTC
mm4__chr7 4.80 Mbp_chr7_4802406 GTACCTCGGGGCACGCTGGCTGGGCCCCCTCCCCGGGCACCTCGGGGCACGCTGGCTGGGCCCCCTCCCCGGGCACCTCG
GGGCACGCTGGCTGGGGGGCCCCCTCCCCAGGTACCTC
mm4__chr7 2.15 Mbp_chr7_2152650 AAGGAGATGGACCCATGGGAGAAAACCTGGGTCTCAGCCCCAGCCCTGACCCCTCCAGGCTCAACTCCCTACCTGCTCGG
CTGGGCTTCCTAAGGCTCAC
mm4__chr7 2.02 Mbp_chr7_2016487 GAGTGCACAAAACTGCCAGGGTCCCTTCCGTGCGGCCTCCTGAGTCTGCACGTACACAAGCTGAGCCCCCGCCCAGGCCC
CGCCTGGCTCCTAGGAGAGACCGACCCAGTG
mm4__chr7 158.59 Mbp_chr7_158588410 AGGCTCGGTCCCAGGAAAGCTCCCGGAGGGCCCAGGGCGTGGGCTGGGGCCAGGCGGGCTCTGGGGCAGAGCAGGGTGAG
GCCT
mm4__chr6 87.32 Mbp_chr6_87322673 GTGACCCCGCGAAAGGTAAGCGGCGGCTTCGGTGGTGTTGAGTGGCTGGGCGCCGGCCTGGAGGGGCCAGGGCGGCCGCA
CCATCACGCTCCCGTCTCTG
mm4__chr6 6.81 Mbp_chr6_6810443 GGGTTCTTGCCTACCACCTCTCTCTCCCACCAGCACCAGAAGTGCCAGGGGTGGGGGCAGGTCGGGGGGGTGGGCAGCGG
ATCTCAAGGCTTCT
mm4__chr6 35.21 Mbp_chr6_35214291 CCAGGAGGAGCCGGTAGCATCGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCTGCGAGCTGGGATCC
mm4__chr5 3.43 Mbp_chr5_3429139 CAGGGCTTCTCATGACCAGGTCTCCAAGAAGCTAGAAATTACACAGCCTCTTACCTAAAGAGGCCAAGGCGGGGTCCATG
TCCCACTGCTGTGGAGGGGAGCCTGGCCTGTGATGTTTT
mm4__chr5 174.77 Mbp_chr5_174767307 CGTTGTGCTCCAAACTGACCTACCACAGTCACCTCGCACACCCTCCTGCAGCCTCTCCATCTTATTCCTGCCCACGCCCT
GAGCCCTTCAGGGTTGAGTGCTTATTTTGGAGTTCCTCAGTGCTCTGTGGTCCTCT
mm4__chr4 7.65 Mbp_chr4_7654794 TACTGGGTGCCAATGAGCAGGATAAGTTTGGGGTTGTACCCCAGACAAGCCCCCACCCTGGCCTCCTGAGGAGCCAGACC
CGAGAAAGGAGGCTC
mm4__chr4 107.93 Mbp_chr4_107931828 CTGCTATGCGGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCGCCCGGGCCCAC
GCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTG
mm4__chr3 98.52 Mbp_chr3_98522623 CCACCTCCTGGGCCTCACGGCGGGGCCGGAGAAGGCCAGGGCTGGGGCGGGGCAGCCGTGCTCGGCCCCGGGCCAGGGTC
CCCCGGTACCCGACCAGGCCCCGCCCTAGAGGGCTCGGCCCTGGGACAAATC
mm4__chr3 184.34 Mbp_chr3_184344901 CCACTCTGCCCACCACTAACGGGCAGCTGGGAACGGCAGCGGCAAGCCTCTGACCTGGCCTCTTCTGGGGTGGTGTCCTT
AGATGAGGATGAGGCAGAGCCAGAGGAACAGTGACCCACATCATGCCT
mm4__chr22 43.21 Mbp_chr22_43207439 CTCCTGCTCCTCTCCCATCACCACCCACCCTCCCTTGCTCCTCCAGGGGCCAGGGCTGGGGCAGGGGCGGTCCTGGCTCA
TCACAGGCCCCATCCCAGGGTTACGTGGATTCCC
mm4__chr22 23.77 Mbp_chr22_23767515 GTCGCCATCTGAGCCATGAGCCCCGGCTGGCCCGAAGGGGCGGGGGCTGGGGCGGCTGCCGAGGGCGGTGGGTGCGCGGG
CGGGTGGGCAGAGGGCGCGGCTGGGCGGCTGCGGGGGTGGGAGGAAGCAGGGTTAA
mm4__chr21 6.10 Mbp_chr21_6101636 GTGGCCAGAGGGGAAGAATCTCCTGCAATGCCCAGCCATCCTGCCCTGTCCCTGGCCCCTTCCGGGAGAGGGACTGACTC
AGTGAAGGGTGGACG
mm4__chr21 5.12 Mbp_chr21_5122032 GCAGGAGGATCAGGAAGTGGTGCAGCAGGAGGCCACCAGGGCGAGAGGGCCCACGCCCTGGAACCTTCCGGAGCAGCTGG
CAGAACCCGGGCTGCGGTGCTGAGACTCAGACGCTG
mm4__chr21 43.44 Mbp_chr21_43436599 CGTCCACCCTTCACTGAGTCAGTCCCTCTCCCGGAAGGGGCCAGGGACAGGGCAGGATGGCTGGGCATTGCAGGAGATTC
TTCCCCTCTGGCCAC
mm4__chr21 39.45 Mbp_chr21_39445650 GACTGAGTGACCGGCGATACTGGAAGCCCAGAAAGAGCGTAGCAGGAGGGTCCGGAAGGGCCCAGAGCTGGGGCTGTAAC
AGGGTAACCGGCTG
mm4__chr20 62.49 Mbp_chr20_62487804 GCCTTATCTGGCCGCCTAATCCCCTGGCCTTGGCTGGAGAACACCAGGGCTTTGCCCAGTCCGCCCTCCCTACCCCCTCC
CTGGCCCCTGCAGGCCGTTGGGGCTGGGGGTGGAGGGAGAGGGAGGTGGAGTGAA
mm4__chr20 37.42 Mbp_chr20_37420185 TCAGAAACCCAGAGCAGTGCCTGTCCCAAGTCCTCCAGATGCCCACGCCCAGGCCCCGTCTGGCCCCTGCCTTCTCTGTC
CTCTGTCCTCTGTCTCTGCCGCTATCTGTCTCCGTGTC
mm4__chr2 96.03 Mbp_chr2_96034495 ACGTTTGGAATGCTTGTGCCCTACAGAGAGCCGCAAGAGAACTTCACCTTCCCGGCTCTGCTCAAACATGGCCTCGGCGC
CCCACAGCCCCCGCCCAGGGCCCCTCAGGCCTCAGCTTCCTCGTCAGAC
mm4__chr2 95.79 Mbp_chr2_95790062 GTCTGACGAGGAAGCTGAGGCCTGAGGGGCCCTGGGCGGGGGCTGTGGGGCGCCGAGGCCATGTTTGAGCAGAGCCGGGA
AGGTGAAGTTCTCTTGCGGCTCTCTGTAGGGCACAAGCATTCCAAACGT
mm4__chr2 70.79 Mbp_chr2_70790686 CTGAGGCCGGGCTTTCACCTGCGCACCCGGGGCCGCACGAGGCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCAC
CTGCAACCATTCCCCGGCTGCGTATTCCAATCCCGGCCTCC
mm4__chr2 68.64 Mbp_chr2_68636162 GAGGGCAAAGTGAGGACAGGTAGATATGCATTGTGAGCATGGTGGGCCAGAGGAGTGCAGGGCGGGGGCTGGATTCTTAT
GGGAGCTCTGAGACTGGGATTCTTATGCGAGCTCTGAAATCCACCGGT
mm4__chr2 241.95 Mbp_chr2_241953840 CAAAGGCCTGGAGCAGATGTACCCAGGCCCCAGGGAGGAGCCGGAGGTGCCCAGGCTGTGGGGAGGAGCCGGAGGTGCCC
AGGCCGGGGGGAGGAGCCAGATGTGCCCAGGCCGGGGCTGTCCTGAGGTCTCCGCT
mm4__chr2 238.91 Mbp_chr2_238912654 GCACACTCTGGTAGCCACATCAGGGTGTCCTCCTCCCCCAGCCCTGGCCTCTCCTGGGGTCTCTCCTGGGGCTTTTGTCT
CCAACTCTGGGCATCCAAGGAGCGGGTCACCGACACCTTAGGCTACG
mm4__chr2 124.54 Mbp_chr2_124536694 GGAGACATGGGCTTGGGTAGCCAGAGCGGGCCAGGGAGGGGGCTACAGAGGAAGCAGCACTTCCTGAGGAACTTGAAGAG
CAGGTTGGATTTACGGGG
mm4__chr2 100.98 Mbp_chr2_100981373 TGCCTCAAGATATGGGAGTCATAGCCCCGAAGAGCCCAGAGCAGGGGCTGCAGGCCCCTCCTGCCATCCCTCCCAAACAG
TGTTTCCTGGAAGCGGAG
mm4__chr19 52.74 Mbp_chr19_52735158 GCGGAGAGACCTTGCCTTTTAGAACCGGCAGAGGGCAGGGCCGGGGCGGGATCTGCGCGTCTCTCAGCCTCGCTCCCAGC
GTCTCTGTTTTCAGGTTTTGGAGGCGAGAGCGCCAGAAAGTCTGAGGCAT
mm4__chr19 50.46 Mbp_chr19_50462029 GACACCTCTCATAGACCGCGTGGTCGTGGCTGGGTACTCGGCAGCCCTCAACTGTGCTGTCAGAGGCCACCCGAAGGTGC
CAGGGCAGGGACCCAGATCTGCGTGTGTGTTGCCTTGTGGGGAATCTTCC
mm4__chr19 15.21 Mbp_chr19_15213827 ACAGTTCCCAACATGGTGGGGGTGGGCTTGAGCCCCCTCCCCGGCCCCTGCTGGCTGCCTTGGCTGGGGACTGCATTTCT
CCTCC
mm4__chr19 1.09 Mbp_chr19_1092186 CTCATTCACCCGGAGACAGGGACAGGGGGCTGACACCCCTCAGTCACCCAGAGACCAGGGAGAAGCAGAGACCTTCGACT
CTGAGAGGCTCCCACCCTGGGCAGTTCTGGGGAGGCGGGTGACTCAGCACCAACCTCTCC
mm4__chr17 8.00 Mbp_chr17_8002622 GGGGCTCTCATCCTCCACTAGCCCGCCCCTCCCTACCTAATTAAGGACCCTAATCAGCTTGGGGAGATTAAGGGCTCTGG
CCGGCTGTACCCACGCCCCCGCCCTGGCCTGGGCTGGCAAGGAAGACCTGTGGGCG
mm4__chr17 47.53 Mbp_chr17_47531868 ACACCTGTGTGGGGCTTTCCCCCCCGCCCCGGACCCTTCTGGGCTTGATAGTGTTCCGGGGGAGGCTGGAGGGTGTTGGG
GGAGGACGGAGAGGTCATGGGAGTCCCCGCTCTGCCTCAACCACCTGACTC
mm4__chr17 40.02 Mbp_chr17_40021545 CTTTAGGCAGGAAGGTGGGGAGTAGCCCCATGCCCCAGCCCGGGCCTCTCCGGGCATGTTTTGGGGGAAGGAAGGAAGGA
AGGTCAGAGCTGGTGAGTCAATGGC
mm4__chr17 38.26 Mbp_chr17_38256549 TGAGTCAGGTGGTTAGGCAGAGCGGGGACTCCCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAACA
CTATCAAGCCCAGAAGGGTCCGGGGCGGGGGGGAAAGCCCCACACAGGTGT
mm4__chr17 36.71 Mbp_chr17_36706305 CCTACGTGTGTGCTCCAAGTCTTGAGAGAATAAAGGCTACTTCTTAGATCAGACTGGGACCTCTCTGTTCCTGGCCTCTT
GCCCCCGCCCTGCCATCTGCCGGCCCCAAGAGGGCAGCACAGGTGGGTCTTTTCT
mm4__chr16 88.94 Mbp_chr16_88939826 CTTCCTGCCTCCTCCCCTGCCGCGAAAGCGATGCCAGGGGCAGGTGAGGGTCGGACGCGCTCAGGCCCAGCCCTCGCCCC
TTCAGGTCTGGGGGACACAGTGGAGTCCCAGCCTCAAGAA
mm4__chr16 73.03 Mbp_chr16_73033611 AACTTTTCACCCGGGGTCAGACAGCTGCGATGCTCTGTCCCCAGCAGAGGCCAGCGCCGGGGCCCCTTCCACCTCACACT
TTCACAGTTTTTTTCCTGCCTGCCAGGAGGCCAGCCTGTTAAAGGA
mm4__chr16 67.29 Mbp_chr16_67291570 AGAGACAGTGTGAGGAGGGGGAGAGGGGTAGGGCCCCCAGGGGCCACTGTCTCTGCCTGAAGTACCCTGGGAGGGGGCTC
AGGCATCCTCACGAGGAGGCGCAGGGCTCGGCCTGGCTCCAGAGCCTCACCTTGG
mm4__chr16 544.23 Kbp_chr16_544225 TCAGAGCACTGAGCAGAGCCCCCCATGGTATCCCCCGCCCTGGGCACAGCAGGAGGAGCTGCCGACCAAGTCTGGCACCC
CGGTGTGCCCCGAGAGCTGTCTGGGCACAG
mm4__chr16 2.93 Mbp_chr16_2932576 TAACTGGGACATGGGCTTGGGTTCGAGGAGGCCACGTGGCTCCTGACACAGCCTTAAGGATCTGAGGTGGCCCCTGCCCT
GGGCACCTGGGGTGGGAAGGGAGGTGCAGGGAACAGGGAGACTT
mm4__chr15 45.15 Mbp_chr15_45147753 ATAGGAGAGGCTGGACAGGGGCTGATCTGTTGGAGATGGGGAAGCCCTGGGACCAGGTCTCCAGCCATGGCAGATGCCCA
GAAGTGCCCAGGCCGAGGTCAGGAAGCAGAGCGACCCTTGCTGTGTCCAGAAG
mm4__chr15 31.78 Mbp_chr15_31778052 AGGACAGCAGGATCTAGGGGTATCATCAGTCTCCAGAAGAGGCCTGGGCTGGGGGATTGATCTGCCCAGTCATCAGCACC
ATGGGAATGGCCGAATGTGCAGAGA
mm4__chr14 101.15 Mbp_chr14_101151542 GTGGGGCAGAGTGAGTCATGGCAGCGCCACCCACCTCGCCGGGTGAGGCCAGGGCGTGGGCTCTTGCCTGAACACTCCAG
GGCTTGCTTC
mm4__chr14 100.65 Mbp_chr14_100654771 GATGGGATTCCTGTGTCGGGGCTGGGAGGCAGCAGGGCCGGGCAGCGTTGTGAGGAGCTTTCCAGAAGAGGCGGGGGCAG
GGGCTGAGGACCCGAGGACCAC
mm4__chr12 124.56 Mbp_chr12_124564356 CCACCTAGGGACACAGACCTTCTGTCACCCTTGGCCTGGGCCTAAAAAACCTGGCTCTCCCCAGGCAGCAGCTCAAGCTT
GGGGCCCTCCTTGCCCCTCCCCTGGGCACCTCAGGCAGGAGTTACCAGCTCCCAC
mm4__chr11 64.35 Mbp_chr11_64348410 GTCTCAGGTTGTCCACTGGGTGGGGAAGCCTCCCAGGCTTGGGGGAGGGGGTTCTAGGNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTATTCACCGTGCCTCCTACCCCAGTACT
mm4__chr10 71.72 Mbp_chr10_71715200 CTGGAAATGCCCTGCCTCTTGCCACTCTGTCACAGCACCCGCCCTGGCCTCTGAAGGGAGCCTCCTCGATCATCTTCAAT
CCCTTCCTGATTGATCCCAACGTGGGTGCCCTGAAGAGGGGCCACCAAG
mm4__chr10 70.51 Mbp_chr10_70512759 CTGGGAGCACTGTCTCCTTGTAGCCCACAAGAGGCCAGGGCAGGGCCCAAGTACAGTGTGGAAGTCCAGCCTG
mm4__chr10 49.62 Mbp_chr10_49616703 CTTTCCCCACCTACATGCCCTTGCTTCTAGAACAGTCCTGAGTGGGACTGGCCCCCAGCATTTGCCTGGCCCCAGCCCTG
CCCTCTACTGGCACAAGGCCCAGGCTACAGCTCCACCCCCAGCTTGCTGAGGATCCCAG
mm4__chr1 3.77 Mbp_chr1_3772576 CTAGCCCTTCCCCGCATGGCCCCGCCCCCCTCTCGCCCCGCCCCCTCTCGCCCCGCCCCCGGCCCCTTCTGGACCAGGCG
CCTCCCCGCTACTGGCCCCAGACCTTCC
mm3__chr17 28.52 Mbp_chr17_28524632 GAGCAAGTCCAGGGCCACTGCCCAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGACAAGTATCCTGGCTT
TGGCTTTGAGG
mm3__chr15 90.20 Mbp_chr15_90201080 AAACCAACGCCACTAGCAGAGGGTCCCAGAGGAGCCGGAAGGGGCCAGGGCCTGGGCGGCGGTGGGAGGGTCTGAGCAGT
G
mm3__chr1 10.77 Mbp_chr1_10767437 TTTTCCGCCTTCCCAGATCCTAGGCAGATCCCTGGAGGGGACAGGGCGGGGGCGATGGGAGCACAGGCTTCCCCTATTGC
A

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

Version 4.7 - Documentation  - Contact us - Downloads/local installation - Paper - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.