CRISPOR

Micro-homology scoring of potential deletions

Guide: GGCTCCGGCATGTGCAAGGC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	(Wild-type sequence)
491.4	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGC----TTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`4 bp deleted → frameshift`
377.6	`CCGCGCTCGTCGTCGACAACGGCTCCGGC---------------TTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`15 bp deleted → no frameshift`
315.0	`CCGCGCTCGTCGTCGACAACGGCT---------------------TCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`21 bp deleted → no frameshift`
313.2	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGC-------------GACGATGCCCCCCGGGCCGTCTTCCCC`	`13 bp deleted → frameshift`
255.2	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGC---------GGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`9 bp deleted → no frameshift`
255.2	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGC---------TTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`9 bp deleted → no frameshift`
219.6	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGG------------GCGACGATGCCCCCCGGGCCGTCTTCCCC`	`12 bp deleted → no frameshift`
198.8	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGC--------------GGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`14 bp deleted → frameshift`
190.2	`CCGCGCTCGTCGTCGACAACGGCTCCGG-----------------------GCGACGATGCCCCCCGGGCCGTCTTCCCC`	`23 bp deleted → frameshift`
180.6	`CCGCGCTCGTCGTCGACAACGGCTCCGGC------------------------GACGATGCCCCCCGGGCCGTCTTCCCC`	`24 bp deleted → no frameshift`
162.8	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGC------------------GACGATGCCCCCCGGGCCGTCTTCCCC`	`18 bp deleted → no frameshift`
147.2	`CCGCGCTCGTCGTCGACAACGGCTCCGGC--------------------GGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`20 bp deleted → frameshift`
141.0	`CCGCGCTCGTCGTCGACAACGG-----------------------------GCGACGATGCCCCCCGGGCCGTCTTCCCC`	`29 bp deleted → frameshift`
140.0	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGC---------------------CCCCCGGGCCGTCTTCCCC`	`21 bp deleted → no frameshift`
140.0	`CCGCGCTCGTCGTCGACAACGGCTCCG---------------------CGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`21 bp deleted → no frameshift`
136.5	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGC--------------------------CCCCCGGGCCGTCTTCCCC`	`26 bp deleted → frameshift`
133.8	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGC------------------------------CGTCTTCCCC`	`30 bp deleted → no frameshift`
133.8	`CCGCGCTCGTCGTCGACAACGGC------------------------------GACGATGCCCCCCGGGCCGTCTTCCCC`	`30 bp deleted → no frameshift`
109.2	`CCGCGCTCGTCGTCGACAACGGC--------------------------GGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`26 bp deleted → frameshift`
108.0	`CCGCGCTCGTCGTCGACAACGGCTCCGG----------------------------------------GCCGTCTTCCCC`	`40 bp deleted → frameshift`
103.6	`CCGCGCTCGTCGTCGACAACGGCTCCG---------------------------ACGATGCCCCCCGGGCCGTCTTCCCC`	`27 bp deleted → no frameshift`
103.6	`CCGCGCTCGTCGTCGACAACG---------------------------CGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`27 bp deleted → no frameshift`
103.6	`CCGCGCTCGTCGTCG---------------------------GCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`27 bp deleted → no frameshift`
99.9	`CCGCGCTCGTCGTCGACAACGGCTC----------------------GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`22 bp deleted → frameshift`
99.4	`CCGCGCTCGTCGTCGAC---------------------------------------GATGCCCCCCGGGCCGTCTTCCCC`	`39 bp deleted → no frameshift`
98.8	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATG----------------------------CCCCCCGGGCCGTCTTCCCC`	`28 bp deleted → frameshift`
96.0	`CCGCGCTCGTCGTCG---------------------------------CGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`33 bp deleted → no frameshift`
94.0	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGG-----------------------------GCCGTCTTCCCC`	`29 bp deleted → frameshift`
89.2	`CCGCGCTCGTCGTCGACAACGGCTCCG------------------------------ATGCCCCCCGGGCCGTCTTCCCC`	`30 bp deleted → no frameshift`
89.2	`CCGCGCTCGTCG------------------------------GCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`30 bp deleted → no frameshift`
84.0	`CCGCG---------------------------------------------GGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`45 bp deleted → no frameshift`
82.5	`CCGCGCTCGTCGTCGACAACG------------------------------------ATGCCCCCCGGGCCGTCTTCCCC`	`36 bp deleted → no frameshift`
82.5	`CCGCGCTCGTCG------------------------------------CGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`36 bp deleted → no frameshift`
80.8	`CCGCGCTCGTCGTCGACAACGGCTCCGGC--------------------------------CCCCCGGGCCGTCTTCCCC`	`32 bp deleted → frameshift`
77.4	`CCGCGCTCGTCGTCGACAACGGCTCCGGC-----------------------------------------CGTCTTCCCC`	`41 bp deleted → frameshift`
76.8	`CCGCGCTCGTCGTCGACAACG---------------------------------ACGATGCCCCCCGGGCCGTCTTCCCC`	`33 bp deleted → no frameshift`
76.8	`CCGCGCTCG---------------------------------GCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`33 bp deleted → no frameshift`
75.0	`CCGCGCT--------------------------------------TCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`38 bp deleted → frameshift`
71.0	`CCGCGCTCG---------------------------------------CGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`39 bp deleted → no frameshift`
69.6	`CCGCGC-------------------------------------------GGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`43 bp deleted → frameshift`
69.6	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGC-----------------------------------CGTCTTCCCC`	`35 bp deleted → frameshift`
69.6	`CCGCGCTCGTCGTCG-----------------------------------GGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`35 bp deleted → frameshift`
66.0	`CCGCGCTCGTCGTCGACAACGGCTCC------------------------------------CCCCGGGCCGTCTTCCCC`	`36 bp deleted → no frameshift`
63.0	`CCGCGCTCGTCGTCGACAACGGCTCCG---------------------------------------------TCTTCCCC`	`45 bp deleted → no frameshift`
62.8	`CCGCGCTCGTCGTCGACAACGGCTCC-------------------------------------CCCGGGCCGTCTTCCCC`	`37 bp deleted → frameshift`
62.8	`CCGCG-------------------------------------GCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`37 bp deleted → frameshift`
61.0	`CCGCGCTCGTCGTCGA------------------------------------------TGCCCCCCGGGCCGTCTTCCCC`	`42 bp deleted → no frameshift`
60.0	`CCGCGCTCGTCGTCGACAACGGCTCC--------------------------------------CCGGGCCGTCTTCCCC`	`38 bp deleted → frameshift`
60.0	`CCGCGCTCGTCGTCGACAACGGC--------------------------------------CCCCCGGGCCGTCTTCCCC`	`38 bp deleted → frameshift`
60.0	`CCGCGCTCGTCGTCGACAACGG----------------------------------------------GCCGTCTTCCCC`	`46 bp deleted → frameshift`
60.0	`CCGCGCTCGTCG--------------------------------------GGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`38 bp deleted → frameshift`
57.0	`CCGCGCTCGTCGTCGACAACGGC-----------------------------------------------CGTCTTCCCC`	`47 bp deleted → frameshift`
56.8	`CCGCGCTCGTCGTCGACAACGGCTCC---------------------------------------CGGGCCGTCTTCCCC`	`39 bp deleted → no frameshift`
56.8	`CCG---------------------------------------GCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`39 bp deleted → no frameshift`
54.0	`CCGC----------------------------------------TTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`40 bp deleted → frameshift`
51.6	`CCGCGCTCG-----------------------------------------GGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`41 bp deleted → frameshift`
51.6	`CCGCG-------------------------------------------------ACGATGCCCCCCGGGCCGTCTTCCCC`	`49 bp deleted → frameshift`
48.8	`CCGCGCTCGTCG------------------------------------------ACGATGCCCCCCGGGCCGTCTTCCCC`	`42 bp deleted → no frameshift`
42.0	`CCGCGCTCGTCG---------------------------------------------ATGCCCCCCGGGCCGTCTTCCCC`	`45 bp deleted → no frameshift`
42.0	`CCGCGCTCG---------------------------------------------ACGATGCCCCCCGGGCCGTCTTCCCC`	`45 bp deleted → no frameshift`
40.5	`CCGCGCTCGTCGTCGACAACGGCTCCGGCATGT----------------------------------------CTTCCCC`	`40 bp deleted → frameshift`
38.0	`CCGCGC-----------------------------------------------GACGATGCCCCCCGGGCCGTCTTCCCC`	`47 bp deleted → frameshift`
38.0	`CCG-----------------------------------------------GGCGACGATGCCCCCCGGGCCGTCTTCCCC`	`47 bp deleted → frameshift`
37.0	`CCGCGCTCGTCGTCGACAACGGCTCC----------------------------------------------------CC`	`52 bp deleted → frameshift`
36.4	`CCGCGCTCG------------------------------------------------ATGCCCCCCGGGCCGTCTTCCCC`	`48 bp deleted → no frameshift`
35.0	`CCGCGCTCGTCGTC------------------------------------------------------------TTCCCC`	`60 bp deleted → no frameshift`
31.2	`CCGCGCTCGTCGTCGACAACG---------------------------------------------------TCTTCCCC`	`51 bp deleted → no frameshift`
31.2	`CCG---------------------------------------------------ACGATGCCCCCCGGGCCGTCTTCCCC`	`51 bp deleted → no frameshift`
30.1	`CCGCGCTCGTC---------------------------------------------------------------TTCCCC`	`63 bp deleted → no frameshift`
29.6	`CCGCGCTCGTCGTCG----------------------------------------------------GGCCGTCTTCCCC`	`52 bp deleted → frameshift`
29.6	`CCGCG----------------------------------------------------ATGCCCCCCGGGCCGTCTTCCCC`	`52 bp deleted → frameshift`
28.4	`CCGCGCTCGTCGTCGACAACGGCTCC-----------------------------------------------------C`	`53 bp deleted → frameshift`
26.8	`CCGCGCTCGTCGTCGACAACGGCTCC------------------------------------------------------`	`54 bp deleted → no frameshift`
26.8	`CCG------------------------------------------------------ATGCCCCCCGGGCCGTCTTCCCC`	`54 bp deleted → no frameshift`
25.8	`CCGCGCTCGTCGTCGACAACGGCTC-------------------------------------------------TTCCCC`	`49 bp deleted → frameshift`
25.6	`CCGCGCTCGTCG-------------------------------------------------------GGCCGTCTTCCCC`	`55 bp deleted → frameshift`
25.6	`CCGCGC-------------------------------------------------------CCCCCGGGCCGTCTTCCCC`	`55 bp deleted → frameshift`
24.6	`CCG----------------------------------------------------------------GGCCGTCTTCCCC`	`64 bp deleted → frameshift`
23.4	`CCGCGCTCGTCGTCGACAACGGCT---------------------------------------------------TCCCC`	`51 bp deleted → no frameshift`
23.2	`CCGCGCTCGTCGTCG---------------------------------------------------------TCTTCCCC`	`57 bp deleted → no frameshift`
23.2	`CCGC---------------------------------------------------------CCCCCGGGCCGTCTTCCCC`	`57 bp deleted → no frameshift`
22.0	`CCGCGCTCG----------------------------------------------------------GGCCGTCTTCCCC`	`58 bp deleted → frameshift`
20.0	`CC------------------------------------------------------------CCCCGGGCCGTCTTCCCC`	`60 bp deleted → no frameshift`
19.2	`CCG---------------------------------------------------------------------TCTTCCCC`	`69 bp deleted → no frameshift`
18.8	`CC-------------------------------------------------------------CCCGGGCCGTCTTCCCC`	`61 bp deleted → frameshift`
18.0	`CCGCG--------------------------------------------------------------GGCCGTCTTCCCC`	`62 bp deleted → frameshift`
18.0	`CC--------------------------------------------------------------CCGGGCCGTCTTCCCC`	`62 bp deleted → frameshift`
17.2	`CC---------------------------------------------------------------CGGGCCGTCTTCCCC`	`63 bp deleted → no frameshift`
16.4	`CCGCGC----------------------------------------------------------------CGTCTTCCCC`	`64 bp deleted → frameshift`
14.8	`CCGC------------------------------------------------------------------CGTCTTCCCC`	`66 bp deleted → no frameshift`
14.0	`CCGCG-------------------------------------------------------------------TCTTCCCC`	`67 bp deleted → frameshift`
12.9	`CCGCGCTCGTCGTC---------------------------------------------------------------CCC`	`63 bp deleted → no frameshift`
11.1	`CCGCGCTCGTC------------------------------------------------------------------CCC`	`66 bp deleted → no frameshift`
11.1	`CCGCGCTC------------------------------------------------------------------TTCCCC`	`66 bp deleted → no frameshift`
9.9	`CCGCGCT--------------------------------------------------------------------TCCCC`	`68 bp deleted → frameshift`
9.6	`CCGCGCTC---------------------------------------------------------------------CCC`	`69 bp deleted → no frameshift`
8.8	`CC----------------------------------------------------------------------------CC`	`76 bp deleted → frameshift`
8.4	`CC-----------------------------------------------------------------------------C`	`77 bp deleted → frameshift`
8.0	`CC------------------------------------------------------------------------------`	`78 bp deleted → no frameshift`

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