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Contents:

PCR primers for off-targets of CGCGCGCCGGCGCCCCCTGG CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529739_F TCGTCGGCAGCGTCTGGTGAGCTGCGAGAATAGC 60.1 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529739_R GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT 60.6 1.00
mm3__chr8 144.28 Mbp_chr8_144277747_F Primer3: not found at this Tm N.d. 0.70
mm3__chr8 144.28 Mbp_chr8_144277747_R Primer3: not found at this Tm N.d. 0.70
mm2__chr20 51.80 Mbp_chr20_51800326_F TCGTCGGCAGCGTCAAGGGCTAGGCTTCAAGCTG 60.0 0.64
mm2__chr20 51.80 Mbp_chr20_51800326_R GTCTCGTGGGCTCGGAGGGAGGGGAGTTGGCTATT 59.9 0.64
mm4__chr12 113.46 Mbp_chr12_113462542_F TCGTCGGCAGCGTCCCTGACCCACAGCCCTATTC 59.8 0.57
mm4__chr12 113.46 Mbp_chr12_113462542_R GTCTCGTGGGCTCGGAGGAACTCGCGTGAACCG 60.0 0.57
mm3__chr19 41.35 Mbp_chr19_41352631_F TCGTCGGCAGCGTCTCCTCTTCCTCCAGCCAGTT 60.1 0.54
mm3__chr19 41.35 Mbp_chr19_41352631_R GTCTCGTGGGCTCGGGCGTGCTAATGGTGGAAACC 59.8 0.54
mm2__chr12 120.47 Mbp_chr12_120469808_F TCGTCGGCAGCGTCCTTAAAGAGAACGCGGCTGC 59.9 0.54
mm2__chr12 120.47 Mbp_chr12_120469808_R GTCTCGTGGGCTCGGGATGGCATCCACACGGCC 61.2 0.54
mm4__chr12 122.87 Mbp_chr12_122867102_F TCGTCGGCAGCGTCCAGGTGGAAGAAGTCTCCCG 59.7 0.50
mm4__chr12 122.87 Mbp_chr12_122867102_R GTCTCGTGGGCTCGGTCTCTTCGCAGTTCGTGTCC 60.0 0.50
mm2__chr18 50.27 Mbp_chr18_50268604_F TCGTCGGCAGCGTCGACTGTGGCTGGGAACTACG 60.3 0.43
mm2__chr18 50.27 Mbp_chr18_50268604_R GTCTCGTGGGCTCGGCTGAAGGCTGAGGCTCTTGG 60.3 0.43
mm2__chr2 104.84 Mbp_chr2_104843191_F Primer3: not found at this Tm N.d. 0.42
mm2__chr2 104.84 Mbp_chr2_104843191_R Primer3: not found at this Tm N.d. 0.42
mm4__chr11 112.28 Mbp_chr11_112280083_F TCGTCGGCAGCGTCGCACTTGGCTCTCAGGGATT 60.0 0.40
mm4__chr11 112.28 Mbp_chr11_112280083_R GTCTCGTGGGCTCGGCTTCTTTGCCGCCCATCCTA 60.1 0.40
mm4__chr7 155.35 Mbp_chr7_155345037_F TCGTCGGCAGCGTCCTAGTCCCCGCCCCTTCG 61.5 0.40
mm4__chr7 155.35 Mbp_chr7_155345037_R GTCTCGTGGGCTCGGACTGCCTTGTGCCTTGAAGT 60.1 0.40
mm4__chrX 47.16 Mbp_chrX_47158275_F Primer3: not found at this Tm N.d. 0.38
mm4__chrX 47.16 Mbp_chrX_47158275_R Primer3: not found at this Tm N.d. 0.38
mm4__chr5 1.05 Mbp_chr5_1053298_F TCGTCGGCAGCGTCCACCCACAAACCCAGGTCTT 60.1 0.38
mm4__chr5 1.05 Mbp_chr5_1053298_R GTCTCGTGGGCTCGGTCCTTTCTTGCAGTGTGCCT 59.8 0.38
mm2__chr11 67.27 Mbp_chr11_67266334_F Primer3: not found at this Tm N.d. 0.37
mm2__chr11 67.27 Mbp_chr11_67266334_R Primer3: not found at this Tm N.d. 0.37
mm4__chr3 58.49 Mbp_chr3_58491951_F Primer3: not found at this Tm N.d. 0.37
mm4__chr3 58.49 Mbp_chr3_58491951_R Primer3: not found at this Tm N.d. 0.37
mm4__chr1 3.57 Mbp_chr1_3565405_F Primer3: not found at this Tm N.d. 0.37
mm4__chr1 3.57 Mbp_chr1_3565405_R Primer3: not found at this Tm N.d. 0.37
mm4__chr11 66.28 Mbp_chr11_66282631_F TCGTCGGCAGCGTCCCCTTCCATGGTGACGGTC 60.0 0.37
mm4__chr11 66.28 Mbp_chr11_66282631_R GTCTCGTGGGCTCGGTGGCGGAGAAGCAGGTACT 60.6 0.37
mm4__chr16 88.07 Mbp_chr16_88068622_F TCGTCGGCAGCGTCCTCACAGAAGAGCCCACTGG 60.0 0.34
mm4__chr16 88.07 Mbp_chr16_88068622_R GTCTCGTGGGCTCGGCAGTCACTCTGTCCTGGGTG 59.6 0.34
mm4__chr19 51.10 Mbp_chr19_51097557_F TCGTCGGCAGCGTCGTGGGACTGAGAGCACCATC 60.1 0.33
mm4__chr19 51.10 Mbp_chr19_51097557_R GTCTCGTGGGCTCGGTGGTGACCACTTGGACTTGG 59.8 0.33
mm4__chr1 205.53 Mbp_chr1_205529125_F TCGTCGGCAGCGTCGCTGCTCCGTCCCTCTCA 61.0 0.33
mm4__chr1 205.53 Mbp_chr1_205529125_R GTCTCGTGGGCTCGGAGGTGGGAGCAAAGATGGC 60.0 0.33
mm4__chr3 46.72 Mbp_chr3_46717875_F Primer3: not found at this Tm N.d. 0.32
mm4__chr3 46.72 Mbp_chr3_46717875_R Primer3: not found at this Tm N.d. 0.32
mm3__chr10 100.01 Mbp_chr10_100010061_F Primer3: not found at this Tm N.d. 0.32
mm3__chr10 100.01 Mbp_chr10_100010061_R Primer3: not found at this Tm N.d. 0.32
mm4__chr17 8.11 Mbp_chr17_8110395_F TCGTCGGCAGCGTCAAGGAACGAGGGATGATGGG 59.1 0.32
mm4__chr17 8.11 Mbp_chr17_8110395_R GTCTCGTGGGCTCGGCCACTGTGCCTGCTGTGG 60.9 0.32
mm4__chr20 728.55 Kbp_chr20_728550_F Primer3: not found at this Tm N.d. 0.31
mm4__chr20 728.55 Kbp_chr20_728550_R Primer3: not found at this Tm N.d. 0.31
mm4__chr1 27.36 Mbp_chr1_27361795_F TCGTCGGCAGCGTCATGGTTGGCAGGACTGTAGC 60.0 0.30
mm4__chr1 27.36 Mbp_chr1_27361795_R GTCTCGTGGGCTCGGGGTACCTGGTGTCCGTGATG 60.1 0.30
mm4__chr20 37.56 Mbp_chr20_37564617_F TCGTCGGCAGCGTCCTGCTCTTTCCGTTCCGCT 60.3 0.29
mm4__chr20 37.56 Mbp_chr20_37564617_R GTCTCGTGGGCTCGGTTCTCCTCCCTGGGTCTCAG 59.9 0.29
mm4__chr7 98.75 Mbp_chr7_98745888_F TCGTCGGCAGCGTCGTGTCTGGCTTGTTCCCTCT 59.6 0.29
mm4__chr7 98.75 Mbp_chr7_98745888_R GTCTCGTGGGCTCGGCAGTTTGGCTACCGCTGGAT 60.3 0.29
mm2__chr7 152.44 Mbp_chr7_152435518_F Primer3: not found at this Tm N.d. 0.29
mm2__chr7 152.44 Mbp_chr7_152435518_R Primer3: not found at this Tm N.d. 0.29
mm4__chr3 132.04 Mbp_chr3_132035060_F TCGTCGGCAGCGTCGGCAAGAGACTGGTTCCGAA 59.9 0.28
mm4__chr3 132.04 Mbp_chr3_132035060_R GTCTCGTGGGCTCGGAATGCTCGTCTCCGTCAGC 60.1 0.28
mm4__chr11 126.06 Mbp_chr11_126062454_F TCGTCGGCAGCGTCAAGCATCAGCATCCAGACCC 60.1 0.28
mm4__chr11 126.06 Mbp_chr11_126062454_R GTCTCGTGGGCTCGGCTTGGCTTAGGGGACGGGAG 61.9 0.28
mm4__chr1 98.05 Mbp_chr1_98054302_F TCGTCGGCAGCGTCCTGAGCCCAATACGCCCG 60.5 0.27
mm4__chr1 98.05 Mbp_chr1_98054302_R GTCTCGTGGGCTCGGGGCACTGGAGGGTGGAGG 61.7 0.27
mm3__chr1 32.71 Mbp_chr1_32712331_F TCGTCGGCAGCGTCTCAGAGGAAGCTGCGTTCAG 60.0 0.27
mm3__chr1 32.71 Mbp_chr1_32712331_R GTCTCGTGGGCTCGGCTGCGGCGGAAAAGACAAC 60.0 0.27
mm3__chr19 1.51 Mbp_chr19_1514009_F Primer3: not found at this Tm N.d. 0.27
mm3__chr19 1.51 Mbp_chr19_1514009_R Primer3: not found at this Tm N.d. 0.27
mm3__chr1 235.10 Mbp_chr1_235104356_F TCGTCGGCAGCGTCATTGGCAGAATCACAGTCGC 58.9 0.26
mm3__chr1 235.10 Mbp_chr1_235104356_R GTCTCGTGGGCTCGGGTTTTCATCAGGGCAGCAGC 60.1 0.26
mm3__chrX 153.83 Mbp_chrX_153830587_F Primer3: not found at this Tm N.d. 0.26
mm3__chrX 153.83 Mbp_chrX_153830587_R Primer3: not found at this Tm N.d. 0.26
mm4__chr19 1.25 Mbp_chr19_1248220_F TCGTCGGCAGCGTCTGTTTTCCTGGACGACAGCC 60.5 0.26
mm4__chr19 1.25 Mbp_chr19_1248220_R GTCTCGTGGGCTCGGAGGGTTTTGGGGTGTACAGC 60.1 0.26
mm4__chr12 111.40 Mbp_chr12_111397093_F TCGTCGGCAGCGTCTGAGGCAGGGGAGTTTAGGA 59.8 0.26
mm4__chr12 111.40 Mbp_chr12_111397093_R GTCTCGTGGGCTCGGCAGGCCCTGAGGGTGGTAA 60.9 0.26
mm4__chr14 103.19 Mbp_chr14_103189096_F Primer3: not found at this Tm N.d. 0.25
mm4__chr14 103.19 Mbp_chr14_103189096_R Primer3: not found at this Tm N.d. 0.25
mm4__chr14 58.40 Mbp_chr14_58395890_F TCGTCGGCAGCGTCCTGGGTGCGAGGCGTCAA 62.6 0.25
mm4__chr14 58.40 Mbp_chr14_58395890_R GTCTCGTGGGCTCGGCACAAGCCGAGCGTTGGG 61.7 0.25
mm3__chr19 47.31 Mbp_chr19_47305436_F TCGTCGGCAGCGTCGACCTGGCTTCCTGAACCTC 60.0 0.24
mm3__chr19 47.31 Mbp_chr19_47305436_R GTCTCGTGGGCTCGGCAGTCTGCGTTACTCACCCA 59.6 0.24
mm3__chr22 18.02 Mbp_chr22_18024600_F Primer3: not found at this Tm N.d. 0.24
mm3__chr22 18.02 Mbp_chr22_18024600_R Primer3: not found at this Tm N.d. 0.24
mm4__chrX 153.64 Mbp_chrX_153644235_F Primer3: not found at this Tm N.d. 0.24
mm4__chrX 153.64 Mbp_chrX_153644235_R Primer3: not found at this Tm N.d. 0.24
mm4__chr13 80.34 Mbp_chr13_80343024_F TCGTCGGCAGCGTCGAATCCATTGAGCCGACGGT 60.4 0.24
mm4__chr13 80.34 Mbp_chr13_80343024_R GTCTCGTGGGCTCGGGTGTCCCGGAGAAGAAAGCA 59.9 0.24
mm2__chr3 50.26 Mbp_chr3_50260140_F TCGTCGGCAGCGTCCTCGACTGGGTGGGGCTC 61.7 0.23
mm2__chr3 50.26 Mbp_chr3_50260140_R GTCTCGTGGGCTCGGCTGACGTCAGCCTCTGTGAC 60.3 0.23
mm3__chr10 100.67 Mbp_chr10_100671172_F TCGTCGGCAGCGTCAGAGAGCCACGAGACCCG 60.7 0.23
mm3__chr10 100.67 Mbp_chr10_100671172_R GTCTCGTGGGCTCGGCAGCCCATCCTCCGGACC 61.8 0.23
mm4__chr9 126.70 Mbp_chr9_126702616_F TCGTCGGCAGCGTCGAAGGCCACCTCTCATCCCT 60.9 0.23
mm4__chr9 126.70 Mbp_chr9_126702616_R GTCTCGTGGGCTCGGTCTGAAGGCAGCTGGAACTG 59.9 0.23
mm4__chr3 133.93 Mbp_chr3_133927716_F Primer3: not found at this Tm N.d. 0.22
mm4__chr3 133.93 Mbp_chr3_133927716_R Primer3: not found at this Tm N.d. 0.22
mm4__chr9 133.59 Mbp_chr9_133589605_F TCGTCGGCAGCGTCCCCCATGAAGGCAAGAACCT 59.9 0.22
mm4__chr9 133.59 Mbp_chr9_133589605_R GTCTCGTGGGCTCGGCCCCACTGCATTGAAATCAGC 60.1 0.22
mm4__chr6 34.03 Mbp_chr6_34027789_F Primer3: not found at this Tm N.d. 0.22
mm4__chr6 34.03 Mbp_chr6_34027789_R Primer3: not found at this Tm N.d. 0.22
mm4__chr17 3.85 Mbp_chr17_3846353_F Primer3: not found at this Tm N.d. 0.22
mm4__chr17 3.85 Mbp_chr17_3846353_R Primer3: not found at this Tm N.d. 0.22
mm4__chr9 124.04 Mbp_chr9_124041146_F Primer3: not found at this Tm N.d. 0.21
mm4__chr9 124.04 Mbp_chr9_124041146_R Primer3: not found at this Tm N.d. 0.21
mm4__chr20 38.17 Mbp_chr20_38165438_F TCGTCGGCAGCGTCCACCCATTGCCCAGTCCC 60.3 0.21
mm4__chr20 38.17 Mbp_chr20_38165438_R GTCTCGTGGGCTCGGGGGCTAGTTGTGTGTCCCTG 60.3 0.21
mm4__chr11 64.25 Mbp_chr11_64251427_F Primer3: not found at this Tm N.d. 0.21
mm4__chr11 64.25 Mbp_chr11_64251427_R Primer3: not found at this Tm N.d. 0.21
mm3__chr7 4.79 Mbp_chr7_4792247_F TCGTCGGCAGCGTCGACTACCAAGGCACAGCTGT 59.9 0.21
mm3__chr7 4.79 Mbp_chr7_4792247_R GTCTCGTGGGCTCGGGGAAGAGTTTGAGCCTGGGG 60.3 0.21
mm4__chr12 57.24 Mbp_chr12_57239210_F TCGTCGGCAGCGTCACAGAGGGTCAAAGGCAGC 59.9 0.21
mm4__chr12 57.24 Mbp_chr12_57239210_R GTCTCGTGGGCTCGGGCCTCTTCCCCGAGCTTG 60.1 0.21
mm4__chr5 669.26 Kbp_chr5_669258_F Primer3: not found at this Tm N.d. 0.21
mm4__chr5 669.26 Kbp_chr5_669258_R Primer3: not found at this Tm N.d. 0.21
mm3__chr5 177.49 Mbp_chr5_177491677_F TCGTCGGCAGCGTCTCATTCAGAGACCCCTCCCC 60.3 0.20
mm3__chr5 177.49 Mbp_chr5_177491677_R GTCTCGTGGGCTCGGCTCAGTCTGTGCTGCGCC 61.1 0.20
mm4__chr2 231.71 Mbp_chr2_231710443_F Primer3: not found at this Tm N.d. 0.20
mm4__chr2 231.71 Mbp_chr2_231710443_R Primer3: not found at this Tm N.d. 0.20
mm4__chr11 71.48 Mbp_chr11_71484456_F TCGTCGGCAGCGTCAAAACCTGGCGCTGCAAAAT 59.8 0.20
mm4__chr11 71.48 Mbp_chr11_71484456_R GTCTCGTGGGCTCGGGCACCTGCACATCCTGTAGT 60.0 0.20
mm4__chr16 180.39 Kbp_chr16_180394_F Primer3: not found at this Tm N.d. 0.19
mm4__chr16 180.39 Kbp_chr16_180394_R Primer3: not found at this Tm N.d. 0.19
mm4__chr6 84.76 Mbp_chr6_84762667_F TCGTCGGCAGCGTCGCTCCCTGCAGATCCACC 59.8 0.19
mm4__chr6 84.76 Mbp_chr6_84762667_R GTCTCGTGGGCTCGGCTTCCCCTCTGGCGTCAC 59.7 0.19
mm4__chr16 31.44 Mbp_chr16_31443183_F TCGTCGGCAGCGTCGGGCATCGAAGGAAGGGAAT 59.8 0.18
mm4__chr16 31.44 Mbp_chr16_31443183_R GTCTCGTGGGCTCGGGACCCAATGTCCCCGTTCAA 60.2 0.18
mm3__chr9 91.95 Mbp_chr9_91950287_F TCGTCGGCAGCGTCCCGATGGGCAGAGCGAGG 62.5 0.18
mm3__chr9 91.95 Mbp_chr9_91950287_R GTCTCGTGGGCTCGGCCCAGCTCCTTGGGTCCC 61.7 0.18
mm4__chr8 4.99 Mbp_chr8_4991676_F TCGTCGGCAGCGTCCTCCTCCCCGGGAAAGCC 61.7 0.18
mm4__chr8 4.99 Mbp_chr8_4991676_R GTCTCGTGGGCTCGGCTAGCAAAGCCCGGGTGAC 60.7 0.18
mm4__chr2 96.55 Mbp_chr2_96550139_F TCGTCGGCAGCGTCAACTGCAGTCCTTCGAGTCC 59.6 0.18
mm4__chr2 96.55 Mbp_chr2_96550139_R GTCTCGTGGGCTCGGTACACGTTCTTCCAGAGGCG 59.7 0.18
mm4__chr6 159.17 Mbp_chr6_159169344_F Primer3: not found at this Tm N.d. 0.18
mm4__chr6 159.17 Mbp_chr6_159169344_R Primer3: not found at this Tm N.d. 0.18
mm3__chr15 70.85 Mbp_chr15_70853835_F TCGTCGGCAGCGTCCTCTGCCCGAGGAGTTGTC 59.7 0.18
mm3__chr15 70.85 Mbp_chr15_70853835_R GTCTCGTGGGCTCGGGAGCGAGGAGGAGCCGAG 61.5 0.18
mm4__chr1 37.51 Mbp_chr1_37514796_F Primer3: not found at this Tm N.d. 0.18
mm4__chr1 37.51 Mbp_chr1_37514796_R Primer3: not found at this Tm N.d. 0.18
mm4__chr3 195.92 Mbp_chr3_195915188_F TCGTCGGCAGCGTCAAACAGAGCCTCATGGAGCC 60.0 0.17
mm4__chr3 195.92 Mbp_chr3_195915188_R GTCTCGTGGGCTCGGCACATCTGGCATGACTGTGC 59.5 0.17
mm4__chr1 206.66 Mbp_chr1_206658645_F TCGTCGGCAGCGTCCTCCTGGGTGGGAAATGTCC 60.0 0.17
mm4__chr1 206.66 Mbp_chr1_206658645_R GTCTCGTGGGCTCGGGCGACTGAAGAGAAGGCAGA 59.7 0.17
mm4__chr16 66.61 Mbp_chr16_66608712_F TCGTCGGCAGCGTCTGCCTCTAGAGCAGGTCTGT 59.9 0.17
mm4__chr16 66.61 Mbp_chr16_66608712_R GTCTCGTGGGCTCGGCAGCTCATTCCTGTAGGCCC 60.1 0.17
mm4__chr1 34.87 Mbp_chr1_34866509_F TCGTCGGCAGCGTCCCGACAAGGTCAAATCCCCA 59.9 0.17
mm4__chr1 34.87 Mbp_chr1_34866509_R GTCTCGTGGGCTCGGCTGCTGCGAGCTGTTGAGAA 60.9 0.17
mm4__chr3 138.92 Mbp_chr3_138915555_F TCGTCGGCAGCGTCGAAATCTAAGGCTGGCAGCG 59.3 0.17
mm4__chr3 138.92 Mbp_chr3_138915555_R GTCTCGTGGGCTCGGGGGTTTCCAGCTCGCCTG 60.7 0.17
mm4__chr9 67.23 Mbp_chr9_67234167_F TCGTCGGCAGCGTCAGTGATGCCTCTCCTTTGCC 60.0 0.17
mm4__chr9 67.23 Mbp_chr9_67234167_R GTCTCGTGGGCTCGGGTACGCGAAGGACACAGC 58.5 0.17
mm4__chr9 61.10 Mbp_chr9_61096956_F TCGTCGGCAGCGTCCTGCGTACGCGAAGGACA 60.1 0.17
mm4__chr9 61.10 Mbp_chr9_61096956_R GTCTCGTGGGCTCGGAGTGATGCCTCTCCTTTGCC 60.0 0.17
mm4__chr3 160.23 Mbp_chr3_160225998_F Primer3: not found at this Tm N.d. 0.17
mm4__chr3 160.23 Mbp_chr3_160225998_R Primer3: not found at this Tm N.d. 0.17
mm3__chr3 196.53 Mbp_chr3_196528930_F Primer3: not found at this Tm N.d. 0.17
mm3__chr3 196.53 Mbp_chr3_196528930_R Primer3: not found at this Tm N.d. 0.17
mm4__chr9 137.42 Mbp_chr9_137423370_F TCGTCGGCAGCGTCCCTGAGGGTCTGGAGTGGG 60.9 0.17
mm4__chr9 137.42 Mbp_chr9_137423370_R GTCTCGTGGGCTCGGCAAGCGCCAGGTGCAACC 62.3 0.17
mm4__chr19 55.38 Mbp_chr19_55377907_F TCGTCGGCAGCGTCTCTGCTTGTTCTGGCGAGTG 60.6 0.16
mm4__chr19 55.38 Mbp_chr19_55377907_R GTCTCGTGGGCTCGGAAACCGGGCATATGCAGCAC 61.6 0.16
mm4__chr17 47.25 Mbp_chr17_47253808_F Primer3: not found at this Tm N.d. 0.16
mm4__chr17 47.25 Mbp_chr17_47253808_R Primer3: not found at this Tm N.d. 0.16
mm3__chr15 101.01 Mbp_chr15_101008562_F TCGTCGGCAGCGTCTCTGTACGTGGACAATCCCG 59.4 0.16
mm3__chr15 101.01 Mbp_chr15_101008562_R GTCTCGTGGGCTCGGCTGCGTCTCACAGGCAATCT 60.3 0.16
mm4__chr21 44.51 Mbp_chr21_44510660_F TCGTCGGCAGCGTCCAGGTGCTGCAGGAAAGAGA 59.9 0.16
mm4__chr21 44.51 Mbp_chr21_44510660_R GTCTCGTGGGCTCGGGAGCTGTGTCCTCACCTGTG 60.3 0.16
mm3__chr2 25.28 Mbp_chr2_25277395_F TCGTCGGCAGCGTCTTGCTCTGCCCGGTGAGG 62.0 0.15
mm3__chr2 25.28 Mbp_chr2_25277395_R GTCTCGTGGGCTCGGAGTGGTTCCCAGCGCCAC 62.6 0.15
mm4__chr3 49.17 Mbp_chr3_49171185_F TCGTCGGCAGCGTCTTCCTGCTTTATGCCCAGGT 59.2 0.15
mm4__chr3 49.17 Mbp_chr3_49171185_R GTCTCGTGGGCTCGGTCCCAGGAGCCCAATTACAG 59.0 0.15
mm4__chr6 118.90 Mbp_chr6_118895206_F TCGTCGGCAGCGTCTTCTGGCTTCCTCGACCAAC 59.9 0.15
mm4__chr6 118.90 Mbp_chr6_118895206_R GTCTCGTGGGCTCGGGAGGAGGACCGGCCGAAC 62.1 0.15
mm4__chr1 2.48 Mbp_chr1_2479269_F TCGTCGGCAGCGTCGGCTTTGCTGATGGTCAGGA 60.3 0.15
mm4__chr1 2.48 Mbp_chr1_2479269_R GTCTCGTGGGCTCGGGAGGCTCTTGTGACCTCAGG 59.7 0.15
mm4__chr1 3.45 Mbp_chr1_3454398_F TCGTCGGCAGCGTCGAAGGACAGTGACGCAGGAG 60.3 0.15
mm4__chr1 3.45 Mbp_chr1_3454398_R GTCTCGTGGGCTCGGCTCGATTCCCGCCGAGCC 62.9 0.15
mm4__chr22 21.55 Mbp_chr22_21551057_F TCGTCGGCAGCGTCGGTCTCCAGACTTCGCCTG 59.7 0.15
mm4__chr22 21.55 Mbp_chr22_21551057_R GTCTCGTGGGCTCGGCAAGAGGCGGAAGGGGCA 62.0 0.15
mm4__chr22 21.38 Mbp_chr22_21383754_F TCGTCGGCAGCGTCCAAGAGGCGGAAGGGGCA 62.0 0.15
mm4__chr22 21.38 Mbp_chr22_21383754_R GTCTCGTGGGCTCGGGGTCTCCAGACTTCGCCTG 59.7 0.15
mm4__chr22 18.61 Mbp_chr22_18611515_F TCGTCGGCAGCGTCGGTCTCCAGACTTCGCCTG 59.7 0.15
mm4__chr22 18.61 Mbp_chr22_18611515_R GTCTCGTGGGCTCGGCAAGAGGCGGAAGGGGCA 62.0 0.15
mm4__chr22 30.39 Mbp_chr22_30387492_F TCGTCGGCAGCGTCCACCAGGAGGAACGGAGTG 59.7 0.15
mm4__chr22 30.39 Mbp_chr22_30387492_R GTCTCGTGGGCTCGGGTTTTGCCCCTCTCCAGCTC 60.9 0.15
mm4__chr19 40.43 Mbp_chr19_40425423_F TCGTCGGCAGCGTCTCTTCGTCCCCTTCCCCG 60.6 0.15
mm4__chr19 40.43 Mbp_chr19_40425423_R GTCTCGTGGGCTCGGGTCAGAGGGGCCAGAATCC 59.4 0.15
mm4__chr1 9.50 Mbp_chr1_9495407_F TCGTCGGCAGCGTCCAGCCCCGCCTCAAGTAG 59.8 0.15
mm4__chr1 9.50 Mbp_chr1_9495407_R GTCTCGTGGGCTCGGGGCGCATCCTCTTCCGAG 60.2 0.15
mm3__chr16 402.09 Kbp_chr16_402093_F TCGTCGGCAGCGTCGTACCGCCACCTCTTCTGC 60.4 0.14
mm3__chr16 402.09 Kbp_chr16_402093_R GTCTCGTGGGCTCGGACAGGTTTCCTAACACGCGA 59.6 0.14
mm4__chr17 46.82 Mbp_chr17_46822491_F TCGTCGGCAGCGTCGCACCGAGTAAGCGCATAGT 60.5 0.14
mm4__chr17 46.82 Mbp_chr17_46822491_R GTCTCGTGGGCTCGGCCTCCCGCCTTGCTATCAG 59.9 0.14
mm4__chr14 104.72 Mbp_chr14_104723725_F TCGTCGGCAGCGTCAGTTGCGGGGACACAAAGTC 60.8 0.14
mm4__chr14 104.72 Mbp_chr14_104723725_R GTCTCGTGGGCTCGGCACTCTCGAGGAGCGCAC 60.2 0.14
mm3__chr17 27.35 Mbp_chr17_27353942_F TCGTCGGCAGCGTCCTCCTCCCAGCCCCTCTC 60.4 0.14
mm3__chr17 27.35 Mbp_chr17_27353942_R GTCTCGTGGGCTCGGTCTCTGCCCCTCCCTCTTC 59.9 0.14
mm4__chr19 47.39 Mbp_chr19_47391601_F Primer3: not found at this Tm N.d. 0.14
mm4__chr19 47.39 Mbp_chr19_47391601_R Primer3: not found at this Tm N.d. 0.14
mm4__chr1 227.32 Mbp_chr1_227318624_F Primer3: not found at this Tm N.d. 0.14
mm4__chr1 227.32 Mbp_chr1_227318624_R Primer3: not found at this Tm N.d. 0.14
mm4__chr10 14.03 Mbp_chr10_14032613_F TCGTCGGCAGCGTCCTGTTGCTGCCTGGGAACTA 59.9 0.14
mm4__chr10 14.03 Mbp_chr10_14032613_R GTCTCGTGGGCTCGGAGAACACCGCATCCACCAAA 60.1 0.14
mm4__chr9 38.07 Mbp_chr9_38068028_F TCGTCGGCAGCGTCTGAGCAGTTTCTTGCCTCCG 60.6 0.13
mm4__chr9 38.07 Mbp_chr9_38068028_R GTCTCGTGGGCTCGGCCGCCTCATCAAAGTGGAGA 59.7 0.13
mm4__chr12 93.57 Mbp_chr12_93571624_F TCGTCGGCAGCGTCAGTCCGAGAGTGGAGGTGTC 60.6 0.13
mm4__chr12 93.57 Mbp_chr12_93571624_R GTCTCGTGGGCTCGGATCATTGCCTCGACCCCATC 59.8 0.13
mm4__chr11 44.95 Mbp_chr11_44951053_F TCGTCGGCAGCGTCAAACCAGGCTCCCGGCTC 62.0 0.13
mm4__chr11 44.95 Mbp_chr11_44951053_R GTCTCGTGGGCTCGGGGAAAGTCATCCTGGGCGG 60.4 0.13
mm4__chr12 49.13 Mbp_chr12_49130377_F TCGTCGGCAGCGTCTGCGGCACAGGATGAATGAG 60.7 0.13
mm4__chr12 49.13 Mbp_chr12_49130377_R GTCTCGTGGGCTCGGTTTGCAGACGTGGCCATTTC 59.6 0.13
mm2__chr11 280.71 Kbp_chr11_280710_F Primer3: not found at this Tm N.d. 0.13
mm2__chr11 280.71 Kbp_chr11_280710_R Primer3: not found at this Tm N.d. 0.13
mm4__chr12 121.81 Mbp_chr12_121812134_F TCGTCGGCAGCGTCCAGGGAGTCGCCGAGTGC 62.8 0.13
mm4__chr12 121.81 Mbp_chr12_121812134_R GTCTCGTGGGCTCGGCCTAGGATGGGACGGGGTC 60.8 0.13
mm4__chr6 31.58 Mbp_chr6_31580997_F TCGTCGGCAGCGTCGGTACAGAGAGCTGCGCAG 60.5 0.13
mm4__chr6 31.58 Mbp_chr6_31580997_R GTCTCGTGGGCTCGGCGCAGGACGGCCTCTATTAC 60.3 0.13
mm4__chr16 67.28 Mbp_chr16_67279452_F TCGTCGGCAGCGTCCCCGCACCTTGTCTTCCG 60.7 0.12
mm4__chr16 67.28 Mbp_chr16_67279452_R GTCTCGTGGGCTCGGCTGGGAAGGGAGGGCAGT 60.6 0.12
mm2__chr13 93.23 Mbp_chr13_93227028_F TCGTCGGCAGCGTCCATACACACTCGCTCTCGCT 59.9 0.12
mm2__chr13 93.23 Mbp_chr13_93227028_R GTCTCGTGGGCTCGGAGAGCTGCAATCCGAGTTCC 60.1 0.12
mm3__chr2 11.79 Mbp_chr2_11785087_F TCGTCGGCAGCGTCAGATCACGAAAGGTACCGCG 60.1 0.12
mm3__chr2 11.79 Mbp_chr2_11785087_R GTCTCGTGGGCTCGGGACACGCACTCCTTGGAGAA 59.9 0.12
mm3__chr4 119.63 Mbp_chr4_119627340_F Primer3: not found at this Tm N.d. 0.12
mm3__chr4 119.63 Mbp_chr4_119627340_R Primer3: not found at this Tm N.d. 0.12
mm4__chr20 3.82 Mbp_chr20_3820245_F TCGTCGGCAGCGTCCGGCCTTTTCCGCAGACT 60.3 0.12
mm4__chr20 3.82 Mbp_chr20_3820245_R GTCTCGTGGGCTCGGGTTGCCCTAGATCAGCGGAG 60.2 0.12
mm4__chrX 111.68 Mbp_chrX_111681089_F TCGTCGGCAGCGTCAGTCTTTTGCCCCACCCAAC 60.7 0.12
mm4__chrX 111.68 Mbp_chrX_111681089_R GTCTCGTGGGCTCGGGACTGCCTTCTGTGAGGGAG 59.7 0.12
mm4__chr11 131.69 Mbp_chr11_131691698_F Primer3: not found at this Tm N.d. 0.12
mm4__chr11 131.69 Mbp_chr11_131691698_R Primer3: not found at this Tm N.d. 0.12
mm4__chr16 86.34 Mbp_chr16_86340889_F TCGTCGGCAGCGTCGTTTCTGTGGGGCTCCTACC 60.0 0.12
mm4__chr16 86.34 Mbp_chr16_86340889_R GTCTCGTGGGCTCGGTCTGCAGGTGCTGAGATGG 59.3 0.12
mm4__chr14 59.48 Mbp_chr14_59483936_F TCGTCGGCAGCGTCGCAGGGGCAGTGGATATTGA 59.8 0.11
mm4__chr14 59.48 Mbp_chr14_59483936_R GTCTCGTGGGCTCGGCGCTTCGCTTTGGACTTCG 59.8 0.11
mm4__chr3 37.45 Mbp_chr3_37452032_F Primer3: not found at this Tm N.d. 0.11
mm4__chr3 37.45 Mbp_chr3_37452032_R Primer3: not found at this Tm N.d. 0.11
mm4__chr11 925.70 Kbp_chr11_925704_F Primer3: not found at this Tm N.d. 0.11
mm4__chr11 925.70 Kbp_chr11_925704_R Primer3: not found at this Tm N.d. 0.11
mm4__chr6 149.55 Mbp_chr6_149546355_F TCGTCGGCAGCGTCGTTTTGTGCCCCGTTTGTGT 60.1 0.11
mm4__chr6 149.55 Mbp_chr6_149546355_R GTCTCGTGGGCTCGGGGAGGAGTCCGCCCAAATAG 59.8 0.11
mm4__chr13 52.74 Mbp_chr13_52738960_F TCGTCGGCAGCGTCGATGGGAGAGGGGAGCTCA 60.0 0.11
mm4__chr13 52.74 Mbp_chr13_52738960_R GTCTCGTGGGCTCGGGTAAGTCCAGAGGGCGGC 59.8 0.11
mm3__chr6 3.16 Mbp_chr6_3157653_F Primer3: not found at this Tm N.d. 0.11
mm3__chr6 3.16 Mbp_chr6_3157653_R Primer3: not found at this Tm N.d. 0.11
mm4__chr12 2.04 Mbp_chr12_2035060_F TCGTCGGCAGCGTCCACGTGGAAACGGTAAAGGC 59.7 0.11
mm4__chr12 2.04 Mbp_chr12_2035060_R GTCTCGTGGGCTCGGGGTCTCCGCGCATCTCCG 62.9 0.11
mm4__chr11 62.61 Mbp_chr11_62612578_F Primer3: not found at this Tm N.d. 0.11
mm4__chr11 62.61 Mbp_chr11_62612578_R Primer3: not found at this Tm N.d. 0.11
mm3__chr19 18.39 Mbp_chr19_18388570_F TCGTCGGCAGCGTCACTGCTGGCAGAATCTTCGT 59.6 0.11
mm3__chr19 18.39 Mbp_chr19_18388570_R GTCTCGTGGGCTCGGGACAGTGGTCCCCGTTGC 60.6 0.11
mm4__chr8 115.59 Mbp_chr8_115587083_F TCGTCGGCAGCGTCTGTTTTCTCCCGATGCAGGA 59.3 0.11
mm4__chr8 115.59 Mbp_chr8_115587083_R GTCTCGTGGGCTCGGGGCTGTGGAAACCAAGGGAT 60.2 0.11
mm4__chr17 75.75 Mbp_chr17_75753712_F TCGTCGGCAGCGTCGATCCCGGGAGCTGGAGA 60.1 0.11
mm4__chr17 75.75 Mbp_chr17_75753712_R GTCTCGTGGGCTCGGTCGAACTTCCAGCCGCAG 60.0 0.11
mm4__chr1 206.63 Mbp_chr1_206634457_F TCGTCGGCAGCGTCTGGGAACTGTGTGCTATGCT 59.3 0.11
mm4__chr1 206.63 Mbp_chr1_206634457_R GTCTCGTGGGCTCGGGTCGGACCGTCCAGATTCTG 60.1 0.11
mm4__chr15 74.78 Mbp_chr15_74781995_F Primer3: not found at this Tm N.d. 0.11
mm4__chr15 74.78 Mbp_chr15_74781995_R Primer3: not found at this Tm N.d. 0.11
mm4__chr2 176.08 Mbp_chr2_176079980_F TCGTCGGCAGCGTCCAATTCGGAGCAGGTTCCCT 60.0 0.10
mm4__chr2 176.08 Mbp_chr2_176079980_R GTCTCGTGGGCTCGGCGCCTCTCTGAGTGTGCC 60.1 0.10
mm4__chr6 30.40 Mbp_chr6_30397041_F TCGTCGGCAGCGTCGAGCGCTCAATCAGAATGCG 60.0 0.10
mm4__chr6 30.40 Mbp_chr6_30397041_R GTCTCGTGGGCTCGGCCTGGTTGGTGAGAAAGGGG 60.2 0.10
mm4__chr18 59.70 Mbp_chr18_59697430_F TCGTCGGCAGCGTCCTTCTTCCCAGCGCCGAG 60.5 0.10
mm4__chr18 59.70 Mbp_chr18_59697430_R GTCTCGTGGGCTCGGATATTCCGGACCCCTGCTCC 61.1 0.10
mm4__chr5 169.08 Mbp_chr5_169081198_F TCGTCGGCAGCGTCAGGTGCCTGCCACTTACAAG 60.2 0.10
mm4__chr5 169.08 Mbp_chr5_169081198_R GTCTCGTGGGCTCGGCTTCCCACCCTGCTGTGTAC 60.3 0.10
mm4__chr19 47.23 Mbp_chr19_47230654_F TCGTCGGCAGCGTCGTCGACCCTCTTCCCCGG 61.4 0.10
mm4__chr19 47.23 Mbp_chr19_47230654_R GTCTCGTGGGCTCGGGGTCTGGCGGGTTTGTTTAC 59.4 0.10
mm4__chr22 24.42 Mbp_chr22_24423871_F Primer3: not found at this Tm N.d. 0.10
mm4__chr22 24.42 Mbp_chr22_24423871_R Primer3: not found at this Tm N.d. 0.10
mm4__chr16 11.36 Mbp_chr16_11356854_F TCGTCGGCAGCGTCCTTCCCTAGGGCAGCTCTTC 59.5 0.09
mm4__chr16 11.36 Mbp_chr16_11356854_R GTCTCGTGGGCTCGGTTGCTCCATTCTGTGCCCTT 59.8 0.09
mm4__chr7 100.48 Mbp_chr7_100483579_F TCGTCGGCAGCGTCTGCACGGGGTACACGGTG 62.3 0.09
mm4__chr7 100.48 Mbp_chr7_100483579_R GTCTCGTGGGCTCGGGTGCTGAGTTCGGTCTCCC 60.0 0.09
mm4__chr9 98.80 Mbp_chr9_98796557_F Primer3: not found at this Tm N.d. 0.09
mm4__chr9 98.80 Mbp_chr9_98796557_R Primer3: not found at this Tm N.d. 0.09
mm4__chr17 8.08 Mbp_chr17_8080109_F Primer3: not found at this Tm N.d. 0.09
mm4__chr17 8.08 Mbp_chr17_8080109_R Primer3: not found at this Tm N.d. 0.09
mm4__chr17 38.61 Mbp_chr17_38606501_F TCGTCGGCAGCGTCGATCCTCCTCTGCTTCTGCC 59.8 0.09
mm4__chr17 38.61 Mbp_chr17_38606501_R GTCTCGTGGGCTCGGCTGCAGGAGGGGCTGCAA 62.3 0.09
mm4__chr19 11.54 Mbp_chr19_11536230_F TCGTCGGCAGCGTCCGAGTGAGTCATCGCGTGA 59.8 0.09
mm4__chr19 11.54 Mbp_chr19_11536230_R GTCTCGTGGGCTCGGGCTACTGCGCATGTGAGGA 60.1 0.09
mm4__chr10 44.39 Mbp_chr10_44386719_F TCGTCGGCAGCGTCGTCCCCAGGCCTTTGCTG 60.6 0.09
mm4__chr10 44.39 Mbp_chr10_44386719_R GTCTCGTGGGCTCGGCAGGAATGGAGCTGGCCAG 60.4 0.09
mm4__chr2 232.49 Mbp_chr2_232487326_F TCGTCGGCAGCGTCCTCCCTGGCTCCTTCTGCTC 61.9 0.09
mm4__chr2 232.49 Mbp_chr2_232487326_R GTCTCGTGGGCTCGGCGTCTCGGGTTCTCCATCAG 59.8 0.09
mm4__chr2 232.35 Mbp_chr2_232351981_F TCGTCGGCAGCGTCCAGGATCGACCACTCCAACC 60.1 0.09
mm4__chr2 232.35 Mbp_chr2_232351981_R GTCTCGTGGGCTCGGGAGGGTGGGCTGTGTATTCG 60.4 0.09
mm4__chr22 17.17 Mbp_chr22_17171952_F TCGTCGGCAGCGTCAGGGAGGAAGGGACAGGAAA 59.8 0.09
mm4__chr22 17.17 Mbp_chr22_17171952_R GTCTCGTGGGCTCGGCTGCAGAGCCAAAGCACAC 59.7 0.09
mm4__chr15 100.92 Mbp_chr15_100919461_F Primer3: not found at this Tm N.d. 0.09
mm4__chr15 100.92 Mbp_chr15_100919461_R Primer3: not found at this Tm N.d. 0.09
mm4__chr20 63.78 Mbp_chr20_63775312_F TCGTCGGCAGCGTCCAGCACGAAGGCGACAGA 60.0 0.09
mm4__chr20 63.78 Mbp_chr20_63775312_R GTCTCGTGGGCTCGGTTCTCACAGGAGTCGTTGGC 59.9 0.09
mm3__chr17 76.87 Mbp_chr17_76868341_F TCGTCGGCAGCGTCGTCCTCGAAGCTGGTCCTC 59.4 0.09
mm3__chr17 76.87 Mbp_chr17_76868341_R GTCTCGTGGGCTCGGCTGCAGCTGCGCTTCCAA 61.3 0.09
mm3__chr18 36.83 Mbp_chr18_36829211_F TCGTCGGCAGCGTCTTCTGGAGCCGGGACTGTAT 60.3 0.09
mm3__chr18 36.83 Mbp_chr18_36829211_R GTCTCGTGGGCTCGGCGGGGTTAGTTCGGAGCTTC 60.4 0.09
mm4__chr9 128.12 Mbp_chr9_128117812_F TCGTCGGCAGCGTCATGAGCGTGCCGAGATTCAT 59.8 0.09
mm4__chr9 128.12 Mbp_chr9_128117812_R GTCTCGTGGGCTCGGCTTCTTGGCGTGTTTTCCCG 60.0 0.09
mm4__chr7 78.02 Mbp_chr7_78019408_F Primer3: not found at this Tm N.d. 0.08
mm4__chr7 78.02 Mbp_chr7_78019408_R Primer3: not found at this Tm N.d. 0.08
mm4__chr2 27.44 Mbp_chr2_27442281_F TCGTCGGCAGCGTCGCTAAAAGCGTCGTGTGTTC 58.0 0.08
mm4__chr2 27.44 Mbp_chr2_27442281_R GTCTCGTGGGCTCGGCAGGGCCGCTGTACCTGG 62.4 0.08
mm4__chr11 117.23 Mbp_chr11_117232552_F Primer3: not found at this Tm N.d. 0.08
mm4__chr11 117.23 Mbp_chr11_117232552_R Primer3: not found at this Tm N.d. 0.08
mm4__chr5 133.55 Mbp_chr5_133553106_F TCGTCGGCAGCGTCCTTGCCATCAGGTCTCTGGG 60.1 0.08
mm4__chr5 133.55 Mbp_chr5_133553106_R GTCTCGTGGGCTCGGAGTCTTGGACAAGCCTAGGC 59.3 0.08
mm4__chr1 1.35 Mbp_chr1_1349414_F TCGTCGGCAGCGTCGCCGTTAAAGGGACCGCC 61.1 0.08
mm4__chr1 1.35 Mbp_chr1_1349414_R GTCTCGTGGGCTCGGCGATGATCGCCTGCGTCT 59.9 0.08
mm4__chr9 100.43 Mbp_chr9_100427179_F Primer3: not found at this Tm N.d. 0.08
mm4__chr9 100.43 Mbp_chr9_100427179_R Primer3: not found at this Tm N.d. 0.08
mm4__chr15 66.88 Mbp_chr15_66882129_F Primer3: not found at this Tm N.d. 0.08
mm4__chr15 66.88 Mbp_chr15_66882129_R Primer3: not found at this Tm N.d. 0.08
mm4__chr11 115.50 Mbp_chr11_115504581_F TCGTCGGCAGCGTCAGACCAATCACAGCGCCG 60.4 0.07
mm4__chr11 115.50 Mbp_chr11_115504581_R GTCTCGTGGGCTCGGTATTTGCATACGCCCCTCCC 59.8 0.07
mm4__chr12 125.19 Mbp_chr12_125186156_F Primer3: not found at this Tm N.d. 0.07
mm4__chr12 125.19 Mbp_chr12_125186156_R Primer3: not found at this Tm N.d. 0.07
mm4__chr7 42.89 Mbp_chr7_42888099_F Primer3: not found at this Tm N.d. 0.07
mm4__chr7 42.89 Mbp_chr7_42888099_R Primer3: not found at this Tm N.d. 0.07
mm4__chr17 9.00 Mbp_chr17_8998187_F TCGTCGGCAGCGTCCAGCATTTACTGTGCGAGCC 59.9 0.07
mm4__chr17 9.00 Mbp_chr17_8998187_R GTCTCGTGGGCTCGGTGAAGGTGCCTCAACCCATC 59.9 0.07
mm4__chr19 3.91 Mbp_chr19_3907927_F Primer3: not found at this Tm N.d. 0.07
mm4__chr19 3.91 Mbp_chr19_3907927_R Primer3: not found at this Tm N.d. 0.07
mm4__chr13 100.59 Mbp_chr13_100588821_F Primer3: not found at this Tm N.d. 0.07
mm4__chr13 100.59 Mbp_chr13_100588821_R Primer3: not found at this Tm N.d. 0.07
mm4__chr22 45.50 Mbp_chr22_45502778_F Primer3: not found at this Tm N.d. 0.07
mm4__chr22 45.50 Mbp_chr22_45502778_R Primer3: not found at this Tm N.d. 0.07
mm3__chr16 57.80 Mbp_chr16_57802560_F TCGTCGGCAGCGTCCGTTCCCATGGCAACCGG 61.4 0.07
mm3__chr16 57.80 Mbp_chr16_57802560_R GTCTCGTGGGCTCGGAAGGAGTGGGAGTGGGAGTG 60.8 0.07
mm4__chr11 65.01 Mbp_chr11_65013666_F TCGTCGGCAGCGTCTTCCGTCATTGGAACCCAGG 59.9 0.07
mm4__chr11 65.01 Mbp_chr11_65013666_R GTCTCGTGGGCTCGGCCTCTGACAGAGCGGAAAGT 59.3 0.07
mm4__chr16 11.80 Mbp_chr16_11797270_F Primer3: not found at this Tm N.d. 0.07
mm4__chr16 11.80 Mbp_chr16_11797270_R Primer3: not found at this Tm N.d. 0.07
mm4__chr10 121.61 Mbp_chr10_121608789_F TCGTCGGCAGCGTCCCCTTTGTTCTCCGGGTTCT 59.5 0.07
mm4__chr10 121.61 Mbp_chr10_121608789_R GTCTCGTGGGCTCGGCTCTACCTTGATGCCAGCCC 60.1 0.07
mm4__chr18 47.25 Mbp_chr18_47247522_F Primer3: not found at this Tm N.d. 0.07
mm4__chr18 47.25 Mbp_chr18_47247522_R Primer3: not found at this Tm N.d. 0.07
mm4__chr12 47.77 Mbp_chr12_47773638_F TCGTCGGCAGCGTCCATTCCTGGGTGCCTCTGAG 60.1 0.06
mm4__chr12 47.77 Mbp_chr12_47773638_R GTCTCGTGGGCTCGGGCAGTGTGCCGTGACAGATA 60.3 0.06
mm4__chr11 134.94 Mbp_chr11_134942385_F TCGTCGGCAGCGTCATGACTCACAGGCAGGGTTG 59.9 0.06
mm4__chr11 134.94 Mbp_chr11_134942385_R GTCTCGTGGGCTCGGCTGATCTAAACCGCGGCTCT 59.8 0.06
mm4__chr17 81.10 Mbp_chr17_81101920_F TCGTCGGCAGCGTCTGCTAGACCCAGGACAGTGG 60.9 0.06
mm4__chr17 81.10 Mbp_chr17_81101920_R GTCTCGTGGGCTCGGCTCCATCCCTGCAGGCAG 59.8 0.06
mm4__chr4 1.01 Mbp_chr4_1011968_F Primer3: not found at this Tm N.d. 0.06
mm4__chr4 1.01 Mbp_chr4_1011968_R Primer3: not found at this Tm N.d. 0.06
mm4__chr7 151.30 Mbp_chr7_151303604_F Primer3: not found at this Tm N.d. 0.06
mm4__chr7 151.30 Mbp_chr7_151303604_R Primer3: not found at this Tm N.d. 0.06
mm4__chr19 5.80 Mbp_chr19_5804225_F Primer3: not found at this Tm N.d. 0.06
mm4__chr19 5.80 Mbp_chr19_5804225_R Primer3: not found at this Tm N.d. 0.06
mm4__chr7 100.45 Mbp_chr7_100450610_F TCGTCGGCAGCGTCCTAGCAGTTCCTCCACCACC 59.7 0.06
mm4__chr7 100.45 Mbp_chr7_100450610_R GTCTCGTGGGCTCGGAACAGCCTGTCTCGTCTTGG 59.9 0.06
mm4__chr19 53.88 Mbp_chr19_53882284_F Primer3: not found at this Tm N.d. 0.06
mm4__chr19 53.88 Mbp_chr19_53882284_R Primer3: not found at this Tm N.d. 0.06
mm4__chr1 16.03 Mbp_chr1_16026918_F TCGTCGGCAGCGTCTTGTTCTCACTTCAGCCCCG 60.2 0.06
mm4__chr1 16.03 Mbp_chr1_16026918_R GTCTCGTGGGCTCGGGCCAGTGGATTTCAAACCCA 58.6 0.06
mm4__chr11 57.35 Mbp_chr11_57349994_F TCGTCGGCAGCGTCTTTCCCAGATTTCAGGGCCC 59.9 0.06
mm4__chr11 57.35 Mbp_chr11_57349994_R GTCTCGTGGGCTCGGGCGGGGAAAGCAGAAAGGAT 60.6 0.06
mm4__chr1 27.73 Mbp_chr1_27725755_F TCGTCGGCAGCGTCGCCTAAGCTCTTCCGAGGTC 59.8 0.05
mm4__chr1 27.73 Mbp_chr1_27725755_R GTCTCGTGGGCTCGGCCCACTCTGATTCGGTCTGG 59.8 0.05
mm3__chr18 44.68 Mbp_chr18_44679426_F TCGTCGGCAGCGTCGCTCTGGTCTGGGAAGCG 60.1 0.05
mm3__chr18 44.68 Mbp_chr18_44679426_R GTCTCGTGGGCTCGGGGAAGGGAGGTGGATCGGAG 61.4 0.05
mm4__chr2 238.16 Mbp_chr2_238158883_F Primer3: not found at this Tm N.d. 0.05
mm4__chr2 238.16 Mbp_chr2_238158883_R Primer3: not found at this Tm N.d. 0.05
mm4__chr18 21.61 Mbp_chr18_21612215_F TCGTCGGCAGCGTCTCCAAGACCAGCCCTCGATA 60.0 0.05
mm4__chr18 21.61 Mbp_chr18_21612215_R GTCTCGTGGGCTCGGCTGGGCCGCACTCTCTTC 60.1 0.05
mm4__chr22 19.72 Mbp_chr22_19718112_F TCGTCGGCAGCGTCCCTGAACTGGGCGGGTAC 59.7 0.05
mm4__chr22 19.72 Mbp_chr22_19718112_R GTCTCGTGGGCTCGGCGGGAGTCGGTAGCCACC 61.8 0.05
mm4__chr8 96.15 Mbp_chr8_96145299_F TCGTCGGCAGCGTCAGCTCCAAGCACAGCTGC 60.6 0.05
mm4__chr8 96.15 Mbp_chr8_96145299_R GTCTCGTGGGCTCGGAGCTCTTCCCTTGCCTTTCG 60.3 0.05
mm4__chr17 18.18 Mbp_chr17_18184153_F Primer3: not found at this Tm N.d. 0.05
mm4__chr17 18.18 Mbp_chr17_18184153_R Primer3: not found at this Tm N.d. 0.05
mm4__chr8 144.69 Mbp_chr8_144685920_F Primer3: not found at this Tm N.d. 0.05
mm4__chr8 144.69 Mbp_chr8_144685920_R Primer3: not found at this Tm N.d. 0.05
mm2__chr7 28.96 Mbp_chr7_28956402_F Primer3: not found at this Tm N.d. 0.05
mm2__chr7 28.96 Mbp_chr7_28956402_R Primer3: not found at this Tm N.d. 0.05
mm3__chr16 49.28 Mbp_chr16_49282326_F Primer3: not found at this Tm N.d. 0.05
mm3__chr16 49.28 Mbp_chr16_49282326_R Primer3: not found at this Tm N.d. 0.05
mm4__chr4 6.27 Mbp_chr4_6270487_F Primer3: not found at this Tm N.d. 0.05
mm4__chr4 6.27 Mbp_chr4_6270487_R Primer3: not found at this Tm N.d. 0.05
mm4__chr1 6.24 Mbp_chr1_6242375_F TCGTCGGCAGCGTCATGTGCAGCAGCTGGAAGTC 60.9 0.05
mm4__chr1 6.24 Mbp_chr1_6242375_R GTCTCGTGGGCTCGGGTGTTAAGGGCATTGTGCGG 60.1 0.05
mm2__chr22 31.35 Mbp_chr22_31345951_F TCGTCGGCAGCGTCCTCCTGCAAACGCGCCTG 62.0 0.05
mm2__chr22 31.35 Mbp_chr22_31345951_R GTCTCGTGGGCTCGGGACCCCTCCTTCTCCTCCC 60.3 0.05
mm4__chr11 65.59 Mbp_chr11_65592407_F TCGTCGGCAGCGTCGGGTGGCCCATAACTTCTCG 60.4 0.04
mm4__chr11 65.59 Mbp_chr11_65592407_R GTCTCGTGGGCTCGGGATCCGCCCTGCCCTGTAC 62.4 0.04
mm4__chr21 29.30 Mbp_chr21_29299115_F TCGTCGGCAGCGTCGACAGGTCCAGTGAAGGCG 60.3 0.04
mm4__chr21 29.30 Mbp_chr21_29299115_R GTCTCGTGGGCTCGGCGGCCACTTCCAGGATCC 59.8 0.04
mm4__chr15 90.22 Mbp_chr15_90219724_F TCGTCGGCAGCGTCTCCTAGACTTGAGTGCCCCA 59.8 0.04
mm4__chr15 90.22 Mbp_chr15_90219724_R GTCTCGTGGGCTCGGGATATCAGTCCCACGCCCAA 59.5 0.04
mm4__chr1 40.32 Mbp_chr1_40316743_F TCGTCGGCAGCGTCGGAAGGGAGACGTGGGTAGG 61.3 0.04
mm4__chr1 40.32 Mbp_chr1_40316743_R GTCTCGTGGGCTCGGGGGGCATACAATGGTGCTCA 60.3 0.04
mm4__chr13 112.94 Mbp_chr13_112943417_F Primer3: not found at this Tm N.d. 0.04
mm4__chr13 112.94 Mbp_chr13_112943417_R Primer3: not found at this Tm N.d. 0.04
mm4__chr2 241.27 Mbp_chr2_241272237_F Primer3: not found at this Tm N.d. 0.04
mm4__chr2 241.27 Mbp_chr2_241272237_R Primer3: not found at this Tm N.d. 0.04
mm4__chr20 62.07 Mbp_chr20_62065917_F Primer3: not found at this Tm N.d. 0.04
mm4__chr20 62.07 Mbp_chr20_62065917_R Primer3: not found at this Tm N.d. 0.04
mm4__chr16 54.29 Mbp_chr16_54286109_F Primer3: not found at this Tm N.d. 0.03
mm4__chr16 54.29 Mbp_chr16_54286109_R Primer3: not found at this Tm N.d. 0.03
mm4__chr1 155.02 Mbp_chr1_155017649_F TCGTCGGCAGCGTCGGTAAGGGGTTGGAAGAGCC 60.0 0.03
mm4__chr1 155.02 Mbp_chr1_155017649_R GTCTCGTGGGCTCGGATGAGGAGCGTGAAGACAGC 60.1 0.03
mm4__chr7 100.68 Mbp_chr7_100675203_F Primer3: not found at this Tm N.d. 0.03
mm4__chr7 100.68 Mbp_chr7_100675203_R Primer3: not found at this Tm N.d. 0.03
mm4__chr13 113.21 Mbp_chr13_113209202_F Primer3: not found at this Tm N.d. 0.03
mm4__chr13 113.21 Mbp_chr13_113209202_R Primer3: not found at this Tm N.d. 0.03
mm4__chr5 126.60 Mbp_chr5_126601342_F TCGTCGGCAGCGTCCGAACTGGCTGCTCCCTG 60.4 0.03
mm4__chr5 126.60 Mbp_chr5_126601342_R GTCTCGTGGGCTCGGGTGAGACGTCTAAGAGGCCG 59.9 0.03
mm4__chr19 1.45 Mbp_chr19_1445337_F Primer3: not found at this Tm N.d. 0.03
mm4__chr19 1.45 Mbp_chr19_1445337_R Primer3: not found at this Tm N.d. 0.03
mm4__chr19 50.31 Mbp_chr19_50314437_F TCGTCGGCAGCGTCTGGGAAGGCTGGAGACCTTA 59.8 0.03
mm4__chr19 50.31 Mbp_chr19_50314437_R GTCTCGTGGGCTCGGCTCCAAGTCCACCTCAGCAA 59.6 0.03
mm4__chr4 125.32 Mbp_chr4_125317217_F TCGTCGGCAGCGTCCAGTTCTCACTACCAGGCGG 60.1 0.03
mm4__chr4 125.32 Mbp_chr4_125317217_R GTCTCGTGGGCTCGGCTCGTCCTGCAGGCGATAG 60.0 0.03
mm4__chr1 2.60 Mbp_chr1_2603278_F TCGTCGGCAGCGTCCCTTCTGCCCTTGCCCTC 60.0 0.03
mm4__chr1 2.60 Mbp_chr1_2603278_R GTCTCGTGGGCTCGGCTCCCTCAGCCGACTCTCA 60.3 0.03
mm4__chr19 55.70 Mbp_chr19_55703889_F TCGTCGGCAGCGTCTTGGAGGAAGCTGGAAAGCG 60.6 0.03
mm4__chr19 55.70 Mbp_chr19_55703889_R GTCTCGTGGGCTCGGCCCAGAAGGAAGGAGCACAA 59.5 0.03
mm4__chr5 139.39 Mbp_chr5_139394404_F Primer3: not found at this Tm N.d. 0.03
mm4__chr5 139.39 Mbp_chr5_139394404_R Primer3: not found at this Tm N.d. 0.03
mm4__chr13 113.45 Mbp_chr13_113445448_F TCGTCGGCAGCGTCTGGAGTCCACACAGCCTCTA 59.8 0.03
mm4__chr13 113.45 Mbp_chr13_113445448_R GTCTCGTGGGCTCGGTGGGTGTTAAAGTGGCCGTT 60.1 0.03
mm4__chr1 40.32 Mbp_chr1_40316651_F TCGTCGGCAGCGTCGAGCTCGAAACCACACCCA 59.9 0.02
mm4__chr1 40.32 Mbp_chr1_40316651_R GTCTCGTGGGCTCGGCCTACCCACGTCTCCCTTCC 61.3 0.02
mm4__chr14 93.12 Mbp_chr14_93115935_F Primer3: not found at this Tm N.d. 0.02
mm4__chr14 93.12 Mbp_chr14_93115935_R Primer3: not found at this Tm N.d. 0.02
mm4__chr21 8.40 Mbp_chr21_8397957_F Primer3: not found at this Tm N.d. 0.02
mm4__chr21 8.40 Mbp_chr21_8397957_R Primer3: not found at this Tm N.d. 0.02
mm4__chr10 6.09 Mbp_chr10_6089199_F Primer3: not found at this Tm N.d. 0.02
mm4__chr10 6.09 Mbp_chr10_6089199_R Primer3: not found at this Tm N.d. 0.02
mm4__chr20 63.25 Mbp_chr20_63254631_F Primer3: not found at this Tm N.d. 0.02
mm4__chr20 63.25 Mbp_chr20_63254631_R Primer3: not found at this Tm N.d. 0.02
mm4__chr9 39.13 Mbp_chr9_39133052_F TCGTCGGCAGCGTCCTGCGTACGCGAAGGACA 60.1 0.02
mm4__chr9 39.13 Mbp_chr9_39133052_R GTCTCGTGGGCTCGGAGTGATGCCTCTCCTTTGCC 60.0 0.02
mm4__chr2 101.39 Mbp_chr2_101387189_F TCGTCGGCAGCGTCGAGAACATCCCGGGTGGC 60.1 0.02
mm4__chr2 101.39 Mbp_chr2_101387189_R GTCTCGTGGGCTCGGCTGCTAGAGGATTCGGGCAG 59.9 0.02
mm4__chr18 76.50 Mbp_chr18_76495354_F Primer3: not found at this Tm N.d. 0.02
mm4__chr18 76.50 Mbp_chr18_76495354_R Primer3: not found at this Tm N.d. 0.02
mm4__chr2 176.16 Mbp_chr2_176157422_F TCGTCGGCAGCGTCGGAAAGTGCAGGGATCCCAA 59.9 0.02
mm4__chr2 176.16 Mbp_chr2_176157422_R GTCTCGTGGGCTCGGTGCCAAAATATTTACCTGCGGC 60.1 0.02
mm4__chr5 180.21 Mbp_chr5_180208757_F Primer3: not found at this Tm N.d. 0.02
mm4__chr5 180.21 Mbp_chr5_180208757_R Primer3: not found at this Tm N.d. 0.02
mm4__chr1 24.93 Mbp_chr1_24929913_F TCGTCGGCAGCGTCGTGCTTGGGTCTACGGGAAT 59.7 0.02
mm4__chr1 24.93 Mbp_chr1_24929913_R GTCTCGTGGGCTCGGTTCTGCTTTCCCGCTTCTCG 60.6 0.02
mm4__chr11 2.88 Mbp_chr11_2884333_F TCGTCGGCAGCGTCAAGCATTTCCCCTTGTCCCG 60.6 0.02
mm4__chr11 2.88 Mbp_chr11_2884333_R GTCTCGTGGGCTCGGGAGCGCAGTTTTCGCCCC 62.1 0.02
mm4__chr10 102.64 Mbp_chr10_102645000_F TCGTCGGCAGCGTCCCAGTCCCACGTGCAGAC 60.3 0.02
mm4__chr10 102.64 Mbp_chr10_102645000_R GTCTCGTGGGCTCGGAGTGGTAGAGGCGGTAGGC 60.7 0.02
mm3__chr5 79.24 Mbp_chr5_79236018_F Primer3: not found at this Tm N.d. 0.02
mm3__chr5 79.24 Mbp_chr5_79236018_R Primer3: not found at this Tm N.d. 0.02
mm4__chr4 3.77 Mbp_chr4_3767712_F TCGTCGGCAGCGTCCCGTCGAGTTCTTCCTGTCG 60.4 0.02
mm4__chr4 3.77 Mbp_chr4_3767712_R GTCTCGTGGGCTCGGATGACCACAGCCAGCACAAA 60.4 0.02
mm4__chr5 3.60 Mbp_chr5_3599487_F TCGTCGGCAGCGTCATGCTGGCCATCATCACCAA 60.0 0.01
mm4__chr5 3.60 Mbp_chr5_3599487_R GTCTCGTGGGCTCGGATCTTCCTGGTCCTTGCTGC 60.0 0.01
mm4__chr5 2.75 Mbp_chr5_2749513_F TCGTCGGCAGCGTCGGCCCAGGTCATCTTGTTCT 59.6 0.01
mm4__chr5 2.75 Mbp_chr5_2749513_R GTCTCGTGGGCTCGGCACCAAGATGACCCTCACCC 60.0 0.01
mm4__chr5 1.88 Mbp_chr5_1879632_F TCGTCGGCAGCGTCTTCTCGTCTGCGCACTTGTT 60.5 0.01
mm4__chr5 1.88 Mbp_chr5_1879632_R GTCTCGTGGGCTCGGATGCTGGCCATCATCACCAA 60.0 0.01
mm4__chr16 54.29 Mbp_chr16_54285318_F TCGTCGGCAGCGTCGCTCCCATAAGCGTTTCCCT 60.1 0.01
mm4__chr16 54.29 Mbp_chr16_54285318_R GTCTCGTGGGCTCGGCACCAAGATGACCCTCACCC 60.0 0.01
mm4__chrUn_GL000220v1 158.36 Kbp_chrUn_GL000220v1_158361_F Primer3: not found at this Tm N.d. 0.01
mm4__chrUn_GL000220v1 158.36 Kbp_chrUn_GL000220v1_158361_R Primer3: not found at this Tm N.d. 0.01
mm4__chrUn_GL000220v1 114.39 Kbp_chrUn_GL000220v1_114389_F Primer3: not found at this Tm N.d. 0.01
mm4__chrUn_GL000220v1 114.39 Kbp_chrUn_GL000220v1_114389_R Primer3: not found at this Tm N.d. 0.01
mm4__chr22_KI270733v1_random 176.32 Kbp_chr22_KI270733v1_random_176324_F Primer3: not found at this Tm N.d. 0.01
mm4__chr22_KI270733v1_random 176.32 Kbp_chr22_KI270733v1_random_176324_R Primer3: not found at this Tm N.d. 0.01
mm4__chr22_KI270733v1_random 131.25 Kbp_chr22_KI270733v1_random_131245_F Primer3: not found at this Tm N.d. 0.01
mm4__chr22_KI270733v1_random 131.25 Kbp_chr22_KI270733v1_random_131245_R Primer3: not found at this Tm N.d. 0.01
mm4__chr21 8.44 Mbp_chr21_8442187_F Primer3: not found at this Tm N.d. 0.01
mm4__chr21 8.44 Mbp_chr21_8442187_R Primer3: not found at this Tm N.d. 0.01
mm4__chr21 8.26 Mbp_chr21_8259152_F Primer3: not found at this Tm N.d. 0.01
mm4__chr21 8.26 Mbp_chr21_8259152_R Primer3: not found at this Tm N.d. 0.01
mm4__chr21 8.21 Mbp_chr21_8214923_F Primer3: not found at this Tm N.d. 0.01
mm4__chr21 8.21 Mbp_chr21_8214923_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 226.12 Mbp_chr1_226122274_F Primer3: not found at this Tm N.d. 0.01
mm4__chr1 226.12 Mbp_chr1_226122274_R Primer3: not found at this Tm N.d. 0.01
mm3__chr17 64.13 Mbp_chr17_64129569_F TCGTCGGCAGCGTCCTGGATCCCAACGCCCTTT 60.0 0.01
mm3__chr17 64.13 Mbp_chr17_64129569_R GTCTCGTGGGCTCGGCGTCCTGCACGGCACTTG 61.7 0.01
mm4__chr11 117.32 Mbp_chr11_117316125_F Primer3: not found at this Tm N.d. 0.01
mm4__chr11 117.32 Mbp_chr11_117316125_R Primer3: not found at this Tm N.d. 0.01
mm4__chr10 110.50 Mbp_chr10_110498165_F Primer3: not found at this Tm N.d. 0.01
mm4__chr10 110.50 Mbp_chr10_110498165_R Primer3: not found at this Tm N.d. 0.01
mm4__chr5 177.46 Mbp_chr5_177455312_F Primer3: not found at this Tm N.d. 0.01
mm4__chr5 177.46 Mbp_chr5_177455312_R Primer3: not found at this Tm N.d. 0.01
mm4__chr22 48.49 Mbp_chr22_48489287_F Primer3: not found at this Tm N.d. 0.01
mm4__chr22 48.49 Mbp_chr22_48489287_R Primer3: not found at this Tm N.d. 0.01
mm4__chr19 44.91 Mbp_chr19_44908883_F TCGTCGGCAGCGTCATGACCTGCAGAAGCGCC 60.4 0.01
mm4__chr19 44.91 Mbp_chr19_44908883_R GTCTCGTGGGCTCGGCCAGGGAGCCCACAGTGG 62.0 0.01
mm3__chr7 100.57 Mbp_chr7_100573919_F TCGTCGGCAGCGTCCTCCTGGCGAACCCACCC 62.4 0.01
mm3__chr7 100.57 Mbp_chr7_100573919_R GTCTCGTGGGCTCGGGGGCCTCTGTTTCCGGAATT 60.3 0.01
mm4__chrX 118.35 Mbp_chrX_118346458_F TCGTCGGCAGCGTCCAGATCGTCTGCTCCCTCAG 59.6 0.01
mm4__chrX 118.35 Mbp_chrX_118346458_R GTCTCGTGGGCTCGGTTTATAGGACACACCCGCGG 59.8 0.01
mm4__chr7 22.36 Mbp_chr7_22357196_F Primer3: not found at this Tm N.d. 0.01
mm4__chr7 22.36 Mbp_chr7_22357196_R Primer3: not found at this Tm N.d. 0.01
mm4__chr9 114.15 Mbp_chr9_114154234_F Primer3: not found at this Tm N.d. 0.01
mm4__chr9 114.15 Mbp_chr9_114154234_R Primer3: not found at this Tm N.d. 0.01
mm4__chr12 57.77 Mbp_chr12_57772867_F TCGTCGGCAGCGTCCCATCACGCAGAACTTTGGG 59.4 0.01
mm4__chr12 57.77 Mbp_chr12_57772867_R GTCTCGTGGGCTCGGATATGGGAGAGGCGGAGTCC 60.5 0.01
mm4__chrX 71.07 Mbp_chrX_71068406_F Primer3: not found at this Tm N.d. 0.01
mm4__chrX 71.07 Mbp_chrX_71068406_R Primer3: not found at this Tm N.d. 0.01
mm4__chr7 51.32 Mbp_chr7_51317109_F TCGTCGGCAGCGTCCTCTCTGGGCGGAGTGGG 61.4 0.01
mm4__chr7 51.32 Mbp_chr7_51317109_R GTCTCGTGGGCTCGGTTCTCACCCTCCAACGTCAG 59.3 0.01
mm4__chr19 14.07 Mbp_chr19_14073530_F Primer3: not found at this Tm N.d. 0.01
mm4__chr19 14.07 Mbp_chr19_14073530_R Primer3: not found at this Tm N.d. 0.01
mm4__chr16 69.19 Mbp_chr16_69187448_F Primer3: not found at this Tm N.d. 0.01
mm4__chr16 69.19 Mbp_chr16_69187448_R Primer3: not found at this Tm N.d. 0.01
mm4__chr19 53.88 Mbp_chr19_53882296_F TCGTCGGCAGCGTCCTTTGGCTCTTCCTCCCCAC 60.3 0.01
mm4__chr19 53.88 Mbp_chr19_53882296_R GTCTCGTGGGCTCGGAGCCACAGGGAAATTCCGAG 60.0 0.01
mm4__chr1 151.91 Mbp_chr1_151909418_F Primer3: not found at this Tm N.d. 0.01
mm4__chr1 151.91 Mbp_chr1_151909418_R Primer3: not found at this Tm N.d. 0.01
mm4__chr3 128.49 Mbp_chr3_128488642_F Primer3: not found at this Tm N.d. 0.01
mm4__chr3 128.49 Mbp_chr3_128488642_R Primer3: not found at this Tm N.d. 0.01
mm4__chr12 93.44 Mbp_chr12_93441487_F TCGTCGGCAGCGTCGAGAAGAGGGGCTTGGCTG 60.0 0.01
mm4__chr12 93.44 Mbp_chr12_93441487_R GTCTCGTGGGCTCGGGGGCCTCCATAGCTTCTGAG 59.6 0.01
mm4__chr2 231.40 Mbp_chr2_231395585_F TCGTCGGCAGCGTCATGCACTGTCTACTAGGCGC 59.8 0.00
mm4__chr2 231.40 Mbp_chr2_231395585_R GTCTCGTGGGCTCGGCCAGGTGCCTTAACCCCTTC 60.3 0.00
mm4__chr16 80.80 Mbp_chr16_80804532_F Primer3: not found at this Tm N.d. 0.00
mm4__chr16 80.80 Mbp_chr16_80804532_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 150.49 Mbp_chr5_150486491_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 150.49 Mbp_chr5_150486491_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 157.02 Mbp_chr6_157021731_F Primer3: not found at this Tm N.d. 0.00
mm4__chr6 157.02 Mbp_chr6_157021731_R Primer3: not found at this Tm N.d. 0.00
mm4__chrX 71.62 Mbp_chrX_71616969_F TCGTCGGCAGCGTCGAAGTCTGGGAGGGCGAAAA 59.9 0.00
mm4__chrX 71.62 Mbp_chrX_71616969_R GTCTCGTGGGCTCGGTGCATCAGCTTTGACCGCTA 60.0 0.00
mm4__chr10 110.92 Mbp_chr10_110920016_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 110.92 Mbp_chr10_110920016_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 54.10 Mbp_chr4_54100011_F TCGTCGGCAGCGTCGCCTCCCCGGGAAATTAACA 60.0 0.00
mm4__chr4 54.10 Mbp_chr4_54100011_R GTCTCGTGGGCTCGGCTGCCCGAGTCACAACAGAA 60.2 0.00
mm4__chr16 67.53 Mbp_chr16_67528981_F TCGTCGGCAGCGTCCAGCCATGTGAGTGTCCCG 60.7 0.00
mm4__chr16 67.53 Mbp_chr16_67528981_R GTCTCGTGGGCTCGGGACAGAGCCGCAGATCCC 59.8 0.00
mm4__chr11 65.64 Mbp_chr11_65638091_F TCGTCGGCAGCGTCCTGGAATCCCCGCTGCTG 60.5 0.00
mm4__chr11 65.64 Mbp_chr11_65638091_R GTCTCGTGGGCTCGGGCTCTCCCGCTACCGCTC 62.2 0.00
mm4__chr20 35.00 Mbp_chr20_34995466_F Primer3: not found at this Tm N.d. 0.00
mm4__chr20 35.00 Mbp_chr20_34995466_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 36.17 Mbp_chr1_36172530_F TCGTCGGCAGCGTCCAGTGTGGCTGGAGAAGGAG 60.0 0.00
mm4__chr1 36.17 Mbp_chr1_36172530_R GTCTCGTGGGCTCGGGCGTTGCTCGGCTTTAAGAC 60.1 0.00
mm4__chr6 82.37 Mbp_chr6_82365293_F TCGTCGGCAGCGTCTGCGCAACCTCTTCCTTACC 60.3 0.00
mm4__chr6 82.37 Mbp_chr6_82365293_R GTCTCGTGGGCTCGGGCTGCCGCTGAGGTTGAG 61.1 0.00
mm4__chr1 226.06 Mbp_chr1_226062224_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 226.06 Mbp_chr1_226062224_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 45.57 Mbp_chr7_45574478_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 45.57 Mbp_chr7_45574478_R Primer3: not found at this Tm N.d. 0.00
mm4__chr14 73.79 Mbp_chr14_73787052_F Primer3: not found at this Tm N.d. 0.00
mm4__chr14 73.79 Mbp_chr14_73787052_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 123.15 Mbp_chr12_123150869_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 123.15 Mbp_chr12_123150869_R Primer3: not found at this Tm N.d. 0.00
mm4__chr16 69.57 Mbp_chr16_69565729_F TCGTCGGCAGCGTCACCAGGTCGTTGCAAATACCT 59.9 0.00
mm4__chr16 69.57 Mbp_chr16_69565729_R GTCTCGTGGGCTCGGTGCTGCTGTTCCTGAGCC 59.9 0.00
mm4__chr2 113.16 Mbp_chr2_113157455_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 113.16 Mbp_chr2_113157455_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 180.98 Mbp_chr2_180980511_F TCGTCGGCAGCGTCGTGAGGGGAGGTGTGGAGA 60.2 0.00
mm4__chr2 180.98 Mbp_chr2_180980511_R GTCTCGTGGGCTCGGGTGCTCGCTGCTTGCCTG 62.0 0.00
mm4__chrX 153.97 Mbp_chrX_153972472_F TCGTCGGCAGCGTCCGACAGTGGGAGGCGACG 62.7 0.00
mm4__chrX 153.97 Mbp_chrX_153972472_R GTCTCGTGGGCTCGGCCCGGCTCATTTTGCTGC 59.8 0.00
mm4__chr3 12.29 Mbp_chr3_12287840_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 12.29 Mbp_chr3_12287840_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 13.81 Mbp_chr6_13814244_F TCGTCGGCAGCGTCACTAGCAGGAGGAGGAGCAG 60.3 0.00
mm4__chr6 13.81 Mbp_chr6_13814244_R GTCTCGTGGGCTCGGGGCAGCGAAACCTCAGCA 60.6 0.00
mm4__chr17 9.00 Mbp_chr17_9003318_F TCGTCGGCAGCGTCTTTCTTGAAGGCCTCGGAGG 59.6 0.00
mm4__chr17 9.00 Mbp_chr17_9003318_R GTCTCGTGGGCTCGGAGTGCCTACTTTTCCACGCA 59.8 0.00
mm4__chr17 44.18 Mbp_chr17_44176806_F TCGTCGGCAGCGTCGACCTTGCTCTCTTGTCCCC 60.0 0.00
mm4__chr17 44.18 Mbp_chr17_44176806_R GTCTCGTGGGCTCGGAGAGCCACATGTTGCTCCAG 60.3 0.00
mm4__chr10 61.66 Mbp_chr10_61663236_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 61.66 Mbp_chr10_61663236_R Primer3: not found at this Tm N.d. 0.00
mm4__chr10 46.28 Mbp_chr10_46282969_F TCGTCGGCAGCGTCGAGCCGCCTTCAAGGGATG 60.8 0.00
mm4__chr10 46.28 Mbp_chr10_46282969_R GTCTCGTGGGCTCGGAAAGCCTCTGATCAGCTGGG 59.7 0.00
mm4__chr10 46.11 Mbp_chr10_46106491_F TCGTCGGCAGCGTCCGAGAGGTAGGGGAGCCTAG 60.2 0.00
mm4__chr10 46.11 Mbp_chr10_46106491_R GTCTCGTGGGCTCGGGCAGACAGCCCTCTGATGG 60.1 0.00
mm4__chr16 85.61 Mbp_chr16_85611637_F Primer3: not found at this Tm N.d. 0.00
mm4__chr16 85.61 Mbp_chr16_85611637_R Primer3: not found at this Tm N.d. 0.00
mm2__chr2 26.85 Mbp_chr2_26847940_F TCGTCGGCAGCGTCGCCTGCCGGGAGTTGTAG 60.1 0.00
mm2__chr2 26.85 Mbp_chr2_26847940_R GTCTCGTGGGCTCGGGCTAGCAGGTTAAGGGGAGG 59.5 0.00
mm4__chrX 40.10 Mbp_chrX_40095751_F TCGTCGGCAGCGTCTCTTCGCTCTTCCATTCCCC 59.4 0.00
mm4__chrX 40.10 Mbp_chrX_40095751_R GTCTCGTGGGCTCGGGCGAGGCGTAATTTGCTGTT 59.8 0.00
mm4__chr2 11.34 Mbp_chr2_11344669_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 11.34 Mbp_chr2_11344669_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 109.71 Mbp_chr12_109713546_F TCGTCGGCAGCGTCCTGGTTTTGCCACCCCTAGT 59.8 0.00
mm4__chr12 109.71 Mbp_chr12_109713546_R GTCTCGTGGGCTCGGCTCGCGAGGACTGCAACTC 60.8 0.00
mm4__chr8 143.92 Mbp_chr8_143924497_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 143.92 Mbp_chr8_143924497_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 7.91 Mbp_chr6_7910872_F Primer3: not found at this Tm N.d. 0.00
mm4__chr6 7.91 Mbp_chr6_7910872_R Primer3: not found at this Tm N.d. 0.00
mm4__chr20 63.74 Mbp_chr20_63739513_F TCGTCGGCAGCGTCCTTCCCCGGCTCTGGCAG 62.4 0.00
mm4__chr20 63.74 Mbp_chr20_63739513_R GTCTCGTGGGCTCGGCTGGCCTAGGGAGGGAGG 60.1 0.00
mm4__chr20 1.89 Mbp_chr20_1894757_F TCGTCGGCAGCGTCCTTCCCGGGACAGCAGCC 62.7 0.00
mm4__chr20 1.89 Mbp_chr20_1894757_R GTCTCGTGGGCTCGGCACCTTCTCTGCGAGCGAG 60.5 0.00
mm4__chr17 28.73 Mbp_chr17_28726022_F TCGTCGGCAGCGTCGTAGGGGCAGGCGACAGA 61.0 0.00
mm4__chr17 28.73 Mbp_chr17_28726022_R GTCTCGTGGGCTCGGCAGACCTAGGAGGCGGCC 61.5 0.00
mm4__chr11 73.14 Mbp_chr11_73142698_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 73.14 Mbp_chr11_73142698_R Primer3: not found at this Tm N.d. 0.00
mm4__chr10 21.53 Mbp_chr10_21534820_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 21.53 Mbp_chr10_21534820_R Primer3: not found at this Tm N.d. 0.00
mm4__chr10 117.54 Mbp_chr10_117543784_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 117.54 Mbp_chr10_117543784_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 47.44 Mbp_chr1_47439065_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 47.44 Mbp_chr1_47439065_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 42.38 Mbp_chr1_42380705_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 42.38 Mbp_chr1_42380705_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 34.98 Mbp_chr1_34976766_F TCGTCGGCAGCGTCAACCCCAGGACCATGGTCTT 60.7 0.00
mm4__chr1 34.98 Mbp_chr1_34976766_R GTCTCGTGGGCTCGGCCTTCCTGCTGGTGCTCG 60.4 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529739 TGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT
AGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG
mm2__chr20 51.80 Mbp_chr20_51800326 AAGGGCTAGGCTTCAAGCTGGGCCTCCAGGGGGCGCCCGCGCGCAAGCCGGGTGGGCTGTCCGCGACAACCCTGGTGATC
AATAGCCAACTCCCCTCCCT
mm4__chr12 113.46 Mbp_chr12_113462542 CCTGACCCACAGCCCTATTCCTAGAGAAGCTGACCTTGAGCCTCAAGATTGGCCACCAGGGAGCGCCAGCGTGCAGGTGG
CTCGGTCTCCCTCTCGGTGGCTCCCGGTTCACGCGAGTTCCT
mm3__chr19 41.35 Mbp_chr19_41352631 TCCTCTTCCTCCAGCCAGTTTCTTCTGCCAGTCACTTCCTACCCGTGGCCCCGGCACTCCGGCGCCCCCTGGGGGCCCCC
CTCCCGGCTCCCCTGCCCCTCCGAGCTCACCGTTGTGGGTTTCCACCATTAGCACGC
mm2__chr12 120.47 Mbp_chr12_120469808 CTTAAAGAGAACGCGGCTGCGACCATTGCGCGTGCGCGCAGGCCGGCGCCCCCTGGAGGTGCGAAGGGCCTTCGGGCGCT
GACAGGGAGAGCCTGGGGCCGGGCCGTGTGGATGCCATC
mm4__chr12 122.87 Mbp_chr12_122867102 CAGGTGGAAGAAGTCTCCCGGGAAGGACCGCGCCGGCGCTCACTGGAGGATGAAACCCGGCTGGTGTGGAGGGAGCCGGG
GCGGGGGTCTCTGAGGGAGAGGTGGCGGATATGGGGACACGAACTGCGAAGAGA
mm2__chr18 50.27 Mbp_chr18_50268604 GACTGTGGCTGGGAACTACGGGCTTTCTCGCCCCGGCGCCCCCTGGCGGACCCACCAGCAGGTTGAAGGTGTCCGGCCAG
TGCTGAGCACCAAGAGCCTCAGCCTTCAG
mm4__chr11 112.28 Mbp_chr11_112280083 GCACTTGGCTCTCAGGGATTGGCTCTGAAACCTCAGTGGTAAGGCGTCTTCCAGAGCAGCGGCGCCCCCTGGTGGCACGC
GGTAGGATGGGCGGCAAAGAAG
mm4__chr7 155.35 Mbp_chr7_155345037 CTAGTCCCCGCCCCTTCGCCCCCGCCGCGCCGCACCAGCGCCCCCTGGCGGCCATCCGCGCGCCGCGCGCTCCGACTCCC
GCCCACCCTAGAGAGAGCGCGGAGCCCTGCCGGCGACTGACTTCAAGGCACAAGGCAGT
mm4__chr5 1.05 Mbp_chr5_1053298 CACCCACAAACCCAGGTCTTAGCGAGAAGCCACCAGGGGGTGCCCGCACGCTCAGCAGGCACACTGCAAGAAAGGA
mm4__chr11 66.28 Mbp_chr11_66282631 CCCTTCCATGGTGACGGTCGCCATGGGGACGGCGCGGCTGCTTCCCGGCCGTGCCGACAGTGCCGCAGAGATCGCTCTGG
CGCCCCCTGGCGGCCACATGTGGAGCGGTGGGAGCTGCTCCAGTACCTGCTTCTCCGCCA
mm4__chr16 88.07 Mbp_chr16_88068622 CTCACAGAAGAGCCCACTGGTGCGGGTGCTGGCTGCCTCCTCCAGTGGGCGCCAGCTCGGGGTCTGTGGCAGGAACGTGG
GTGGCTGCGGATCCACCCAGGACAGAGTGACTG
mm4__chr19 51.10 Mbp_chr19_51097557 GTGGGACTGAGAGCACCATCTCCCCGGGAAGTTTGAGATTCTCACAACCTCCCCGTACCTGTACAGGACAGCAACCCTGA
GACCCGGCGCCCCCTGGTGGTCAGACAGTAACATGAACGACCAAGTCCAAGTGGTCACCA
mm4__chr1 205.53 Mbp_chr1_205529125 GCTGCTCCGTCCCTCTCACCACCAGGGGGCGCCGGAACTCCTCCAGCCCAGGCGCGGAGCGGGACGGGGAGGAGCGGCTC
GGCCGCCTCTCTCCCGGGCGGGGACCCCCTGTGGCCTCGGCCATCTTTGCTCCCACCT
mm4__chr17 8.11 Mbp_chr17_8110395 AAGGAACGAGGGATGATGGGGCTCCGGGCTGGCGGTTAGCACCTGAGCCCCCATCCCGGGGCGGCGGCGCCGGGCTCACC
TGGATGGCCAAGGGCACCAGCGCCCCCTGGGGGCTGAGCCACAGCAGGCACAGTGG
mm4__chr1 27.36 Mbp_chr1_27361795 ATGGTTGGCAGGACTGTAGCAAGAAGTGGAGCCAGAAGTGGGCACGGCGTGGTGGCCCTCCAGGGGGCTCTGGCGTGGGC
CTGAAGTGCTGGTAGAGCAGGAAGGTCTCCATCACGGACACCAGGTACC
mm4__chr20 37.56 Mbp_chr20_37564617 CTGCTCTTTCCGTTCCGCTCCCGCAGGGGGCGCCAGCGCTCCGTGGAGAGCGGCTGCGGCCCCAGAGACCACGCTCGGGC
CGCCTGACGCCCAGAGCCCCCAGGAAGGGGAAACTGAGACCCAGGGAGGAGAA
mm4__chr7 98.75 Mbp_chr7_98745888 GTGTCTGGCTTGTTCCCTCTTGTTTGGGAAAAGAACACTCACTTATTCCAAGAGGAGGCTTTCTCACCTGAGCCAGGCTC
ACTCTCTCACCACGGCGCCCCCTGGTGGTGTGGGCTTGTATCCAGCGGTAGCCAAACTG
mm4__chr3 132.04 Mbp_chr3_132035060 GGCAAGAGACTGGTTCCGAAACCTCCGCGCGAGCGCCCCCTGGGGGTTGCTGACGGAGACGAGCATT
mm4__chr11 126.06 Mbp_chr11_126062454 AAGCATCAGCATCCAGACCCCGCACGCAGCCCTGCACCCCCACGCCCTCCAGGGAACGCCGGCCCGCCCGTCCCCGGGAC
CTCCCGTCCCCTAAGCCAAG
mm4__chr1 98.05 Mbp_chr1_98054302 CTGAGCCCAATACGCCCGGAGAAGAGCGCCGGAGCCCCCTGCTGGCCGGACGCGGCAGGCGCGGCGGCCCGCTCGGTCCC
CTCCGCCCCGCGGGCTCTGCCCAGCCCGGACCCGCCTCCACCCTCCAGTGCC
mm3__chr1 32.71 Mbp_chr1_32712331 TCAGAGGAAGCTGCGTTCAGGGTCGTTCCTCCCCAGGTCGGGCAATGCCGAGCACTGCCTCCAGGGGGCGCCGCCACGCC
GACCCCGCGCCCCGGGGGTTGTCTTTTCCGCCGCAG
mm3__chr1 235.10 Mbp_chr1_235104356 ATTGGCAGAATCACAGTCGCGCTGGGGCTCGAGGCTCCCGGAGGCCGGGGGGTAGGCACGGCGCGGACGCACCCTAGGAT
CGCCGCCAGAGGGCGCCAGCGCGCTCCTTCGCGTCTGCTGCTGCCCTGATGAAAAC
mm4__chr19 1.25 Mbp_chr19_1248220 TGTTTTCCTGGACGACAGCCGCTGACTCAGGCCCCTCTTCCCGTCCCCCCCACGCGGGCGCCCCCAGGCGGAAGCACCGG
GCCTGGGGCGACAGGAAGGGGGCGGGGCAGCGAGTCGCGCTGTACACCCCAAAACCCT
mm4__chr12 111.40 Mbp_chr12_111397093 TGAGGCAGGGGAGTTTAGGAGGCCGCTTCCCAGCCCTCGATCGCCCCACAGCCACCAGGGGGCGCCAGAGCCCCGCGCAA
AGCGGCTGCCACCAAGTGGCTGCCACCGCGCGTTACCACCCTCAGGGCCTG
mm4__chr14 58.40 Mbp_chr14_58395890 CTGGGTGCGAGGCGTCAACGCGCAGCTGGGTGCGCAGGAGGCCCCGAGGCCGGATGGGCCGCCGCGCCGACGCCCCCTGC
CGGCCGGCCCGCGCCCAACGCTCGGCTTGTG
mm3__chr19 47.31 Mbp_chr19_47305436 GACCTGGCTTCCTGAACCTCCCCGCTCCTGCGCCCCCTGGAGGTCAGGGCTAGAACAACACGGCATTCCTATAATGCTCC
AAATCGCCACAAATCGACCTTGGAAATCCTGGGTGAGTAACGCAGACTG
mm4__chr13 80.34 Mbp_chr13_80343024 GAATCCATTGAGCCGACGGTGAACGCAGGCGCCGCTGCGCTCGCGGCCCCTCTGGCCACCAGGGGGCGCCCGCCCGGCGC
GGGGCTAAGGCTGCTTTCTTCTCCGGGACAC
mm2__chr3 50.26 Mbp_chr3_50260140 CTCGACTGGGTGGGGCTCCGGCGTTGGCGCATCCGCCTCCGGCCGCCAGGGGGCGCCGCCGCCCGTGCTCTGCCCTGCCT
GGCTGGTCACAGAGGCTGACGTCAG
mm3__chr10 100.67 Mbp_chr10_100671172 AGAGAGCCACGAGACCCGCCGCCTCCGCCGGGGCCGGGTGACTGCGCCGTGCCCGACACGCCGACGTCCACCAGGGGGCG
GCCGCGTGCGGCCCGAGCCTCGGTCTGCGGGTCCGGAGGATGGGCTG
mm4__chr9 126.70 Mbp_chr9_126702616 GAAGGCCACCTCTCATCCCTGGATGCCCGCGGGCACCAGCGCCCCCTAGGGGCAGGCCCCGCCCTGCACCCTCTTCTGGG
CTCTCCATTCACAGCCCTCCCCGGGCAGTTCCAGCTGCCTTCAGA
mm4__chr9 133.59 Mbp_chr9_133589605 CCCCATGAAGGCAAGAACCTGGTTTCCCACTGCAGTCCTCCGGCCACCAGGGGGCGACACAGCGCGCACACACACAGTAG
GGCGGCATTGGTGCTGATTTCAATGCAGTGGGG
mm4__chr20 38.17 Mbp_chr20_38165438 CACCCATTGCCCAGTCCCGGGCCCACGCCGCCAGCGCTGGGGCTCACCCAGGGGACCGGAGCCCGAGGGAGGGACGGCGG
CCGGACGAGGGCGCCCCCTGGGGGAGCGGACAGGGACACACAACTAGCCC
mm3__chr7 4.79 Mbp_chr7_4792247 GACTACCAAGGCACAGCTGTGTCGCACGTTCCGCCCGGTGCCTGGGACGGGCTCAGCCGCCAGGGGGCGCCGCCGCCCCG
AGAGCCTCACGCCCCGCCCCCAGGCTCAAACTCTTCC
mm4__chr12 57.24 Mbp_chr12_57239210 ACAGAGGGTCAAAGGCAGCGGGACTCAGCGGATCGAGCGTGTTGCAGGGGCCGTGGGCTCGGGCGCCCCCTGGAGGCCGA
GCGGCCCCCAAGCTCGGGGAAGAGGC
mm3__chr5 177.49 Mbp_chr5_177491677 TCATTCAGAGACCCCTCCCCAGGGCGGTGCCCTGCAAGGCGCCACAGCCTCGGGAGGGGCCCTGTGCCTGCAGCCCCACC
CCGGCCGCCAGGGGGCGCTGCCGCACGGTTCCGCCGGGCTGGGGCGCAGCACAGACTGAG
mm4__chr11 71.48 Mbp_chr11_71484456 AAAACCTGGCGCTGCAAAATGTGCAGGTGCCCCCGCCTGGGCCGGCGTCCCCTGGGGGTGGGGGTGCAGGGAGCACTGGG
ACAATCACTACAGGATGTGCAGGTGC
mm4__chr6 84.76 Mbp_chr6_84762667 GCTCCCTGCAGATCCACCCGCGGGGCCTGCGGCGAGGGCAGAGGGGTCCCGGGCCGGGCCAGGGAGCGCGCCGGAGACCC
CTTGGGGGAGCCTCCCGGTGACGCCAGAGGGGAAG
mm4__chr16 31.44 Mbp_chr16_31443183 GGGCATCGAAGGAAGGGAATGACGGCGCGACGGCGACCCCTAGGGGCGGGAGGAGGCGCTAGGAGCGGGGCTGGGAAGCG
CCATGGTGCAGGTTGAACGGGGACATTGGGTC
mm3__chr9 91.95 Mbp_chr9_91950287 CCGATGGGCAGAGCGAGGCTTGCGCCGGCGCCCCCTGCCGGGGGAACGCGCGGGCGGCGGGCGGGACCCAAGGAGCTGGG
mm4__chr8 4.99 Mbp_chr8_4991676 CTCCTCCCCGGGAAAGCCCTGCTGCTTGCAAGCGCCTCCGGTGGGCCCTGGAGCGCGCTGCCGAGCCCCGGGGCCCAGCG
CCGACGCCCCCGGGGGGAGGCCCGACCTTGGTCACCCGGGCTTTGCTAG
mm4__chr2 96.55 Mbp_chr2_96550139 AACTGCAGTCCTTCGAGTCCCTGCGAGGGCGGCCGGAGCTGCAAGGACCCCGCCGCCAGGGGGCGCCCGCCGGCCGCGCC
CTCACGAGGTGCCCTTGCAGGTGACCGGGCGCCGCCTCTGGAAGAACGTGTA
mm3__chr15 70.85 Mbp_chr15_70853835 CTCTGCCCGAGGAGTTGTCGCGCGCAGTCAGCGCCCCGAACTCGCGCGCGGCCCGGCGCCCCCTCGGGGCCCACCTCCCG
GGCCAGCCGCCGCGCCTACCTGTGCCCGCGGCTCGGCTCCTCCTCGCTC
mm4__chr3 195.92 Mbp_chr3_195915188 AAACAGAGCCTCATGGAGCCCTGCGCTCTGGTGCCCCCTGGTGGACACCTAGAGTTGTGGTTGGTAACCAACAAATGGAC
CAGTGAGTTCAGTTACTCCTGATTGGTTGGTGTTCGGGCACAGTCATGCCAGATGTG
mm4__chr1 206.66 Mbp_chr1_206658645 CTCCTGGGTGGGAAATGTCCTACCTCCCCAACCCCTGCGGCTGCACTTTCACGGGGCTCCCCCCAGGGGGCGCCAGGCCC
CGCCTCCACCTTTCTCTGCCTTCTCTTCAGTCGC
mm4__chr16 66.61 Mbp_chr16_66608712 TGCCTCTAGAGCAGGTCTGTGAGGCAGGGAACCCGGAGAGGGGTCTCTGGCCACCAGGTGGCGCCGGTGCTCCCCACCGG
GCCTACAGGAATGAGCTG
mm4__chr1 34.87 Mbp_chr1_34866509 CCGACAAGGTCAAATCCCCACATTTTCCACCAAAGCGCGGCGCGTTCGAGCGCCCCCTGGTGGCGCCGTTCTCAACAGCT
CGCAGCAG
mm4__chr3 138.92 Mbp_chr3_138915555 GAAATCTAAGGCTGGCAGCGACGGCTCTCCCTTGGGCGCAACAGCCGCGCCCCCGGCGGCAGGCCCGCCCCACAGCGCCC
CCTGGCGGCCCCGAGCGCGCCGCACTGGAGCCCCGCGCTCCGCAGGCGAGCTGGAAACCC
mm4__chr9 67.23 Mbp_chr9_67234167 AGTGATGCCTCTCCTTTGCCCGTTTCTGCTCAGCAGACTCCGCGTGGACGGTGGTGCGGCACGGTGGCCCCGACGCGGTG
ACCCTCCGAGGTGCCCCCAGTGGGCACCCGCGCTCGGCTGTGTCCTTCGCGTAC
mm4__chr9 61.10 Mbp_chr9_61096956 CTGCGTACGCGAAGGACACAGCCGAGCGCGGGTGCCCACTGGGGGCACCTCGGAGGGTCACCGCGTCGGGGCCACCGTGC
CGCACCACCGTCCACGCGGAGTCTGCTGAGCAGAAACGGGCAAAGGAGAGGCATCACT
mm4__chr9 137.42 Mbp_chr9_137423370 CCTGAGGGTCTGGAGTGGGCGTAGCCGTTTCTGGCGGGGGCGTGGCCTTACCGGGGAGGGGCGGGCGCCTGCCGCCAGGG
GGCGCCGCGGGGCAGCGGGGTTGCACCTGGCGCTTG
mm4__chr19 55.38 Mbp_chr19_55377907 TCTGCTTGTTCTGGCGAGTGGGCCTGGGATAGGGCGGGCGCCTGCACCCCCAGGGGGAGGGTCCGGCGGAGGGCGGGGGC
TGAGCCGTCCGCTCCCTGCAGGTGGGCCAAGCGCCGGGACGTGCTGCATATGCCCGGTTT
mm3__chr15 101.01 Mbp_chr15_101008562 TCTGTACGTGGACAATCCCGCCGCGCTCTTCCCTGTGAGCCGCCTCTTGAGGGGCGGCACTGAGCCCGCGCCCACCGGCG
CCCTCTGGAGGTTGGAGGGTGACTTGCAGGCGCTGTGCACAGATTGCCTGTGAGACGCAG
mm4__chr21 44.51 Mbp_chr21_44510660 CAGGTGCTGCAGGAAAGAGAAAACTCTGGGCCCAACGTCAACGTGCGTTCTCCACGCCCAGCAGGGGGCGCCGTTGCCCG
CCCGAGCCCGGAGAGCCTGGGCTGCAGGGGAGGGCGCCACAGGTGAGGACACAGCTC
mm3__chr2 25.28 Mbp_chr2_25277395 TTGCTCTGCCCGGTGAGGCCCCGGGCTCCCAGCAGGGGGCGCCTGCTCGCGATCAGGTGGCGGCCTGGGGGCGGCCTGGC
GGTCCCGGCGGACCCTGACGCGGCGGGGCTCCGGGGCTCGCGGTGGCGCTGGGAACCACT
mm4__chr3 49.17 Mbp_chr3_49171185 TTCCTGCTTTATGCCCAGGTAAGGGCGCCCCAGGCTGCCCCCACACGACAGAGGCCCAGGCCATGTCCCCAGTGGCCTCC
AGGGGGCGCCGCCGGGAAGCCTGTAATTGGGCTCCTGGGA
mm4__chr6 118.90 Mbp_chr6_118895206 TTCTGGCTTCCTCGACCAACCAGCGGGGGAGGCGGGCGCAGGCTCGGCCCCTCCGCGCCGCGGGCTGGGCGCCGGCGCTC
CCCGGGGGCCGTGGGTCCTGTTCGGCCGGTCCTCCTC
mm4__chr1 2.48 Mbp_chr1_2479269 GGCTTTGCTGATGGTCAGGACCCAGGCATGCCAGGCAAGGCCTCAGCCCAGCAGTTGCCAAGCGTGTAGCCACTTTGGGC
CCGTGGAGAGAGTCGCGCTGCGGAGCCCCCAGGGGGACCTGAGGTCACAAGAGCCTC
mm4__chr1 3.45 Mbp_chr1_3454398 GAAGGACAGTGACGCAGGAGGGAGCCGAGCCGCTCTGGCCCCACGCCTGGCAGGCGACTCCCGGGCCGGCCTCGAGGGGG
CGTGGGCGCGGGGAGGGAAGGCGCAGGCACCGGGCGGCGGCGGCTCGGCGGGAATCGAG
mm4__chr22 21.55 Mbp_chr22_21551057 GGTCTCCAGACTTCGCCTGGCAGGCCTCAGCTCGCCAACGGCCGCCGCCGACCGCCCGGCGCGCCCCTTCCGGCGCCCCC
TGGGGGTCTCCGCGTGCCCCTTCCGCCTCTTG
mm4__chr22 21.38 Mbp_chr22_21383754 CAAGAGGCGGAAGGGGCACGCGGAGACCCCCAGGGGGCGCCGGAAGGGGCGCGCCGGGCGGTCGGCGGCGGCCGTTGGCG
AGCTGAGGCCTGCCAGGCGAAGTCTGGAGACC
mm4__chr22 18.61 Mbp_chr22_18611515 GGTCTCCAGACTTCGCCTGGCAGGCCTCAGCTCGCCAACGGCCGCCGCCGACCGCCCGGCGCGCCCCTTCCGGCGCCCCC
TGGGGGTCTCCGCGTGCCCCTTCCGCCTCTTG
mm4__chr22 30.39 Mbp_chr22_30387492 CACCAGGAGGAACGGAGTGGGCGCTTGGCGGCTCTCGCGCCCAGACTGGGCCGCGGGCGCCCCCTGGTGGCCGCGGAGAG
CTCAGGTGCCCGCGGCTGAGGGAGCTGGAGAGGGGCAAAAC
mm4__chr19 40.43 Mbp_chr19_40425423 TCTTCGTCCCCTTCCCCGCAGTCCGGGGAGTCCGGAACCGCAGGAATTCCGCCCCCACCCCCGACTCCGCCCCCGGGTCC
CGGCGCCCCCTAGCGGTGCCCAGGCCGGCTTTCCCCCGGGGATTCTGGCCCCTCTGAC
mm4__chr1 9.50 Mbp_chr1_9495407 CAGCCCCGCCTCAAGTAGCGCCCCAGAGCGGCCACTCCTCGTGCGGCCACCAGGGGGCGCAGCAGCGCCGGCAGGAAGCG
CGGGAAAGCGGCCGCGATGGCCTCGGAAGAGGATGCGCC
mm3__chr16 402.09 Kbp_chr16_402093 GTACCGCCACCTCTTCTGCCCGGACCTGCTGCGGTGAGCGGGGCCTGGGAAGCGAGCGCAGCCTTGGTGCGGGGTCCGGT
CCGCGGGCGCCGGCGCCCCCACGTGGTCCCCGAGGGCTCGCGTGTTAGGAAACCTGT
mm4__chr17 46.82 Mbp_chr17_46822491 GCACCGAGTAAGCGCATAGTAGGTAAGCACCAGATGTTAATCTAACACACAAAGGAGCGCAGGTGCACGCGGAGGTCGGC
TCAGAGCCCAGCGGCGCGGGCGCGCCCCCTGGCGGCAGTGCTGATAGCAAGGCGGGAGG
mm4__chr14 104.72 Mbp_chr14_104723725 AGTTGCGGGGACACAAAGTCCTCGGAGAGGACGCGCTGCGCGGCTCTTCGCGGCCGCCAGGGGGCGCGCCCGCGCCCAGG
GCACTCGGGGTTCCACGGGGGGCAGGTGCGCTCCTCGAGAGTG
mm3__chr17 27.35 Mbp_chr17_27353942 CTCCTCCCAGCCCCTCTCGGCGAGCGGGCGCAGGCGCGGTGCGGCCCTTCTCGGCCGGCAGGGGGCGCCGGAGCCCGCCG
GGGCCTCTAGGGCCTCCCCAGCCACGGCCCGGGCCGGCGAAGAGGGAGGGGCAGAGA
mm4__chr10 14.03 Mbp_chr10_14032613 CTGTTGCTGCCTGGGAACTACGTTGCCACCACTTCCAGTGAAGGCCACCAGGGGGCGCCAGCAAGGGGGAGCTGCTGGCG
GACACCACCCTAAGACGCCCACCCTTTGGTGGATGCGGTGTTCT
mm4__chr9 38.07 Mbp_chr9_38068028 TGAGCAGTTTCTTGCCTCCGCAGGCCGTCGGGCTCCAGGTGCGGCCGCCCGCGCAGGCGCCCCCCAGGGGGTCCCCGGCC
CCACCGCCCGCGGCGCTCTCCACTTTGATGAGGCGG
mm4__chr12 93.57 Mbp_chr12_93571624 AGTCCGAGAGTGGAGGTGTCCCAGCCCGTAGGGGGCGTCGCCGCGCGGTGGGGGATGGGGTCGAGGCAATGAT
mm4__chr11 44.95 Mbp_chr11_44951053 AAACCAGGCTCCCGGCTCCCCCGCGAGGCTCTCCCGGGGGCCGCCTGCAGGGGGCGCACGCGCGCCCGAGAGAAGGCGCC
GGGGGAGGAAGCCGCGCGGGGAGACCCTGGCGTGGCCCGCGCCGCCCAGGATGACTTTCC
mm4__chr12 49.13 Mbp_chr12_49130377 TGCGGCACAGGATGAATGAGCAATTCCGGGCGCGCGCTCTTGCGCCCCCCGGCGGTGCTGCAGAGGCACGAAATGGCCAC
GTCTGCAAA
mm4__chr12 121.81 Mbp_chr12_121812134 CAGGGAGTCGCCGAGTGCCGGGAAGCTCCGGGCCGGCCCGCGGGCGCCCCCTGCTGGCGCCGGGGAATAGCGCCTGGGCT
TTCTGCGGCCGCGCGCAGTGCGGGGAGCTGGACCCCGTCCCATCCTAGG
mm4__chr6 31.58 Mbp_chr6_31580997 GGTACAGAGAGCTGCGCAGCGTGACCGAGCGGCCCTGGGGGTCCCCGCCGCCAGGGGGCGCCCGGCCCCGGTAGCCGACG
AGACAGTAGAGGTAATAGAGGCCGTCCTGCG
mm4__chr16 67.28 Mbp_chr16_67279452 CCCGCACCTTGTCTTCCGCGCCGGCAGGGCTGTGCCCCGTCGAGACCTGCTCGGGCTGTGGCTCCGTCCTCCGGCCTCCA
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTGCCCTCCCTTCCCAG
mm2__chr13 93.23 Mbp_chr13_93227028 CATACACACTCGCTCTCGCTTGTCCATCTCCCTCCCGGGGGAGCCGGCGCGCGCTCCCACCTTTGCCGCACACTCCGGCG
AGCCGAGCCCGCAGCGCTCCAGGATTCTGCGGCTCGGAACTCGGATTGCAGCTCT
mm3__chr2 11.79 Mbp_chr2_11785087 AGATCACGAAAGGTACCGCGGGCCTCGCGCGGGCGCCCTCTGGTGGCCGCCGGTCAGAAGGCGCAGAGGCTTAGGCTTCT
CCAAGGAGTGCGTGTC
mm4__chr20 3.82 Mbp_chr20_3820245 CGGCCTTTTCCGCAGACTCCGCGCTCCGAGCCGCGGCGCCCCCTCGCGGCCGCCGGGGTCGGCAGCCTGGGGCTCCGCTG
ATCTAGGGCAAC
mm4__chrX 111.68 Mbp_chrX_111681089 AGTCTTTTGCCCCACCCAACCGGTGACACAGCCCGGGGCCCCGCCGCCCCCTGGCGGCCATTACGGATTTCCGCCTCCCT
CACAGAAGGCAGTC
mm4__chr16 86.34 Mbp_chr16_86340889 GTTTCTGTGGGGCTCCTACCTGCCCCCAGGCTGGGCTCTCCCATCCGGGAGAGCGGCGCGCCCCTCGTCCTCACCACCAG
GGGGCGCAGGGGAGCCCCATCTCAGCACCTGCAGA
mm4__chr14 59.48 Mbp_chr14_59483936 GCAGGGGCAGTGGATATTGACGCGGGCCTCGCGGGTGCCCGCAGGGGGCGCCGGCACAAGCCCGAAGTCCAAAGCGAAGC
G
mm4__chr6 149.55 Mbp_chr6_149546355 GTTTTGTGCCCCGTTTGTGTCCGTCTCAAGAGCAGAGCGGCCCTCCGCGCGGCCCTTTTATCCACCAGGGGGCGCCCGCG
GCGGGATCTTCTCTCCACCTATTTGGGCGGACTCCTCC
mm4__chr13 52.74 Mbp_chr13_52738960 GATGGGAGAGGGGAGCTCACGCTGGGCCCGAGGGGCCCGCCGGCAGCCGCGCGCCCCTCGCCGGCCCGCGCTCGGGCTCC
CCCTAGGGGCACCATGGGCGACACGGGGGTCCCCGCGCGCCGCCCTCTGGACTTAC
mm4__chr12 2.04 Mbp_chr12_2035060 CACGTGGAAACGGTAAAGGCCGACGCCGGTTCAGAGCCGCAACCGCGACAGCCCCGTCGTCCACCTCAGGTGCCGGCGCC
CCCTGCTGGTGCGCGGAGATGCGCGGAGACC
mm3__chr19 18.39 Mbp_chr19_18388570 ACTGCTGGCAGAATCTTCGTCCGCACGGCCCCAGCTGGAGTTGCACTTGCGGCCGCAAGCCGCCAGGGGGCGCCGCAGAG
CGCGTGCGCGCAACGGGGACCACTGTC
mm4__chr8 115.59 Mbp_chr8_115587083 TGTTTTCTCCCGATGCAGGATACTGCTGGGGGAGGGCCCCAGACTTCTCTCCGCCAGCTGGCGCCCCCTGCAGGAATCCC
TTGGTTTCCACAGCC
mm4__chr17 75.75 Mbp_chr17_75753712 GATCCCGGGAGCTGGAGACGGGCTCCCCTCGCAGAGCCTACGGCCTTCCCCCGCCTGGCCCTGCTCGGCCCGGCGCCCCC
CGGCGGTGCCAACGCGGCCCTTCGTTGTTCCCAAGGCTGCGGCTGGAAGTTCGA
mm4__chr1 206.63 Mbp_chr1_206634457 TGGGAACTGTGTGCTATGCTGATTTGGGACAATAAGCGTTCTAGTCCCACCAGGTGGCGCCCGCGTTCGGCATTCTGAGA
ACGTTTGGTGTCTTCTGGACTCCAGAATCTGGACGGTCCGAC
mm4__chr2 176.08 Mbp_chr2_176079980 CAATTCGGAGCAGGTTCCCTTCGGCCTCCCGCGCCCCGGGGCGCCCCCTGGCGGCAGCGGCAGCAGCGGGCAACGCGCGG
AGGGCTCAGGGGGCGCACAGGGGACTCCCGGGCACACTCAGAGAGGCG
mm4__chr6 30.40 Mbp_chr6_30397041 GAGCGCTCAATCAGAATGCGAGAGCAGCGCGGCGGCGCCCCCGTGTGGCCACAGGGACGAGGACAGAGGACCGGACCCCG
CTCCCCTTTCTCACCAACCAGG
mm4__chr18 59.70 Mbp_chr18_59697430 CTTCTTCCCAGCGCCGAGCTCCGTCCGGACCAAGCGTCCTGCTCCTCCGCGGCCGCCGCCGCCTTCCCTCTTCCCGGCAG
GGGGCGCCGGAGAGCAGGGGCGTTTGCACCACCTGCACGGGGAGCAGGGGTCCGGAATAT
mm4__chr5 169.08 Mbp_chr5_169081198 AGGTGCCTGCCACTTACAAGAAAACAATTTTCTCAGGTTGAACTCTGCCTCCCGCTCTGGGGCCCCCTGGAGGGGTGCAG
TGCTTTTCTCTTTCATTAAACAAGGGTAGGGGGTACACAGCAGGGTGGGAAG
mm4__chr19 47.23 Mbp_chr19_47230654 GTCGACCCTCTTCCCCGGGGCCGCACTGGCCGCCAGGGGGCGCTGCCGAGCCCGCACCCCATTGTTTGTAAACAAACCCG
CCAGACC
mm4__chr16 11.36 Mbp_chr16_11356854 CTTCCCTAGGGCAGCTCTTCCCCAGGGCAGCTCTGGGAGCTGATGGTCTCTACACGGGCGCTGGCCCCCCCTGCTGGTCT
ATGCACAGAAAATGGGATGACAACCCGTCACCAGAAAACAAGGGCACAGAATGGAGCAA
mm4__chr7 100.48 Mbp_chr7_100483579 TGCACGGGGTACACGGTGCGGGGGCGTGGGCCCCAGGCTGCCGCTGGGGGGCGCCGCCGCACGCCGCTCCGCCGGGGCCG
GGGAGACCGAACTCAGCAC
mm4__chr17 38.61 Mbp_chr17_38606501 GATCCTCCTCTGCTTCTGCCTCGCGACCCCTCACCCCAGCGCGCCTAGACGCGGCTCGGTCGGAGGGTCTGGGCTCCCCG
GCGCCCCCTGCTGGGCCGCCGCCTCGCCCCCGCTCCGGGTTGCAGCCCCTCCTGCAG
mm4__chr19 11.54 Mbp_chr19_11536230 CGAGTGAGTCATCGCGTGACGTCACCGCCGCCTGGACCTCGCCAGGTCCCGCCCCCGCCCCTCCCGGGCCGCCAGGGGGC
GCCGGCTGCAGGTCCTCACATGCGCAGTAGC
mm4__chr10 44.39 Mbp_chr10_44386719 GTCCCCAGGCCTTTGCTGGGCTGGGCCGGGGAGCCTGGCCGCTCACCGGCCGCAGCGCTGGGGCTCCGGCGCCCTCTGGT
GGCGGCGCCTCCCTCCCCGCTCCCAGAGCTCTGGCCAGCTCCATTCCTG
mm4__chr2 232.49 Mbp_chr2_232487326 CTCCCTGGCTCCTTCTGCTCGGTGGCCCGACGGCCCACTCGCCCCTTCCCTAGGGGCGCGCGCCAACGCCCCAGGGTGGC
GGACACACAACCCACCCACTGGACGGCCCTGATGGAGAACCCGAGACG
mm4__chr2 232.35 Mbp_chr2_232351981 CAGGATCGACCACTCCAACCCCGCTCGCTGGCTCCTTCTACTCGGTGGCCCGACGGCTCACCCGCCCCTTCCTCACCGGC
GCGCGCCAACGCCCCAGGGTGGCGAATACACAGCCCACCCTC
mm4__chr22 17.17 Mbp_chr22_17171952 AGGGAGGAAGGGACAGGAAACTGAAGCTGTTTCCGGGGCTGACCCCGGCCAGAGAGAGCAGGGCTCGGGAGAGTCGGCGC
GTCCCGGAGGCGGCGCCCCCTGGAGGCCGGTGTGCTTTGGCTCTGCAG
mm4__chr20 63.78 Mbp_chr20_63775312 CAGCACGAAGGCGACAGAGGGGCCGGCAGGGGGCGCCCGAGCCCGTCTCTGGAGCACAAAGACCCCCGGCCCCTGCGCGA
CCCGGACGAGCTCGCATTCAGCCAACGACTCCTGTGAGAA
mm3__chr17 76.87 Mbp_chr17_76868341 GTCCTCGAAGCTGGTCCTCGGCGTCCCAGCCCCGCACGGCCCCGGGCAGGGCGCACAGCGGGGCTGCGGCCACGAGGTGG
CGCCGGCGCGCCTTGGAAGCGCAGCTGCAG
mm3__chr18 36.83 Mbp_chr18_36829211 TTCTGGAGCCGGGACTGTATCCTTTGCCCGCCTGACAGGGGGCGCCGGCGCCCTCCACGGGACGGCGAGGGGCGAGCCGT
CGCGTGGCAGTCCGAGAGCGGAGGAGAAGCTCCGAACTAACCCCG
mm4__chr9 128.12 Mbp_chr9_128117812 ATGAGCGTGCCGAGATTCATTCCCGGAATTGGCCGCTAGGTGGCAGCAGCAGGCCCTGTTGCCATCTCCTACGAGGCTTG
GCGGGCTCTGGCGCCCTCTGGTGGCTGCGGGAAAACACGCCAAGAAG
mm4__chr2 27.44 Mbp_chr2_27442281 GCTAAAAGCGTCGTGTGTTCGCGCCTTTCCCGCCAGGGGGAGCGGGCCCGGGGCGGAGGCGGGGCCCAGGTGGGAGGGGG
ACCCGGGACGGAGGCGGGGCCCAGGTTGGGGCGGGGCCGGGCCCAGGTACAGCGGCCCTG
mm4__chr5 133.55 Mbp_chr5_133553106 CTTGCCATCAGGTCTCTGGGCCTGGAAAACGTAACTCTCCCTCTGACCTCCAGGGGGCGCGGGAGAGAGGGTGAGTCCCG
CGGTACCCGGAACCCACAGTCTGTATCAAGAGGGAGGAAGCCTAGGCTTGTCCAAGACT
mm4__chr1 1.35 Mbp_chr1_1349414 GCCGTTAAAGGGACCGCCCGGCCCGGGGTCCTGAGCGTGGCCGCGGGGGGGCGCCCGAGCGCGCGGCGTTGACGTCACGG
GGGCGCACGGCGGGGGCGGGGCGAGGATGCGCAGACGCAGGCGATCATCG
mm4__chr11 115.50 Mbp_chr11_115504581 AGACCAATCACAGCGCCGCGAGCCCAACCCGGCTCTCGGAGGGCCTCGCTCCCTCCCCCACCCCGCCCCCTGGCGCTGGC
GTTCGGCGGCGCGCCCGCGCCACCTAGGGGCGGGGCCAAGGGGAGGGGCGTATGCAAATA
mm4__chr17 9.00 Mbp_chr17_8998187 CAGCATTTACTGTGCGAGCCACCTGGGGGCCATTTTTGTTGCCCGAGGTGGTGCGCCTGCGCCACCTGGTGGACTACGGG
AAAGTAGCAGATCGGTGAGATGGGTTGAGGCACCTTCA
mm3__chr16 57.80 Mbp_chr16_57802560 CGTTCCCATGGCAACCGGCTCCGACCCCGGCGGGGGGCGGCGGCGCGCGCCCGCACTCACGCGCACTGGCGCGCACGCGT
CAGCCCGGGCGCGCCCCCGCAGGTCTCCCGCCGGCACTCCCACTCCCACTCCCACTCCTT
mm4__chr11 65.01 Mbp_chr11_65013666 TTCCGTCATTGGAACCCAGGGACGCCCCGCATGTTTCCGAACGGCCTCCAGGGGGCACCGCCGCCCTGAACTGAGAGCCA
CCGGAGCCGGGTAGGGGGAGGCCCAACTTTCCGCTCTGTCAGAGG
mm4__chr10 121.61 Mbp_chr10_121608789 CCCTTTGTTCTCCGGGTTCTGCGCAGGGAGGAGCGCATCGGGCGCCAGCGCCCCCGTGTGGCCGCGAGCACCGCGCGCGC
AAAGCGGGGGCGAGCGCCGCCCGGGGCGTGGGTGACGGGGCTGGCATCAAGGTAGAG
mm4__chr12 47.77 Mbp_chr12_47773638 CATTCCTGGGTGCCTCTGAGACCCGATGCCCGCCCGGGCGCCACCTGGGGGTGCCGACGGCCCGCCACTGCCGGCTGCAC
AGACCTGCCGCGCCGCCCGGGTTATCTGTCACGGCACACTGC
mm4__chr11 134.94 Mbp_chr11_134942385 ATGACTCACAGGCAGGGTTGCAACCACCAGGGGGCGGAGCAGCGCGGCCCTCCCAGCAGGGGCGTAGGACTAGAGTGAGG
AGGGGCGCGAGTGCGCGGCCCACAGAAGAGCCGCGGTTTAGATCAG
mm4__chr17 81.10 Mbp_chr17_81101920 TGCTAGACCCAGGACAGTGGCGGGCGTCTGCGCCCTCTGGCGGGGGCCAGGCCCCATGACGGGGCCAGGGAGTGAGAGCG
GGGCTGTCAGTGGCTGGGGTGCTGCCTGCAGGGATGGAG
mm4__chr7 100.45 Mbp_chr7_100450610 CTAGCAGTTCCTCCACCACCCCCATTAGGCCGTAGGGGCTTCCCCAGCCAGAGGCCAAGGGCGACGCGACCTTAACGCCG
GTCCGCGCACCGCGGCGCCACCTGGAGGTCAGGACCAAGACGAGACAGGCTGTT
mm4__chr1 16.03 Mbp_chr1_16026918 TTGTTCTCACTTCAGCCCCGCTTTGGGTATAGCCACCCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNTGAGAGGCTTCAGGATAACATTACACAGACTTGGGTTTGAAATCCACTGGC
mm4__chr11 57.35 Mbp_chr11_57349994 TTTCCCAGATTTCAGGGCCCTTTGGCCGGCACTGGCCAGGAGCCGCCGCGAGACAGCGCCACCTGGTGGAAGCATCCCAG
GCCGCCACTGGCTTTGTGCCTGAATCCTTTCTGCTTTCCCCGC
mm4__chr1 27.73 Mbp_chr1_27725755 GCCTAAGCTCTTCCGAGGTCCCGGCCGGGAGCCTGTCCCCTCCAGGTGGCGCCCCCGCGTGACTGCGCTTTACATAATCG
CGGCCTGGAGCGTCACAGTGGCGTACTCCAGGCCAGACCGAATCAGAGTGGG
mm3__chr18 44.68 Mbp_chr18_44679426 GCTCTGGTCTGGGAAGCGGCTCTGGGCCCTCCGGGCGAGCAGCTTGGGCGCCGGCGCCCCCTGCGAGGAAGATCGCGGGG
TGACGGTCGGTACCCGTTCGGCTGGGCCGCGCTGCCGCTCCGATCCACCTCCCTTCC
mm4__chr18 21.61 Mbp_chr18_21612215 TCCAAGACCAGCCCTCGATACCCTCAGCCGTCGCAAGGCAACCTCCAGCCGCCAGGGGCCGCAGTCGCGCTCCTCGCGGC
CGGAAGAGAGTGCGGCCCAG
mm4__chr22 19.72 Mbp_chr22_19718112 CCTGAACTGGGCGGGTACCCGCGCTGTTGCGCGTGGGTCCCGGGAGCGGCCTCCGGCGCCCTCTGGCGGGCGGAAGCCGC
GCAGCACCTCTTGGGGGCGGGACGCGCGGTGGCTACCGACTCCCG
mm4__chr8 96.15 Mbp_chr8_96145299 AGCTCCAAGCACAGCTGCTTCCAGGGCTGGTGGCGCAGGCCCTGCCACACGTCGAAGACTTCCCAGCCGGCCGGCGGCGC
CCCCTGCGGGTCCAGGGTCCGCGCGTCCAGCAGTAGGGGCGAAAGGCAAGGGAAGAGCT
mm4__chr1 6.24 Mbp_chr1_6242375 ATGTGCAGCAGCTGGAAGTCCGCGCCTGGCGGGGCGCCCGGAGTTTGGCCCCGAGGGGGCGGGGGCGGGCGCGGGCGCCG
CTTTGTGGGGCCCGCACAATGCCCTTAACAC
mm2__chr22 31.35 Mbp_chr22_31345951 CTCCTGCAAACGCGCCTGCCACCTCCCCTCCCGCCCGCCAGGCGGCGCCGGCGCGCAGCCAAGAAGGGCACGCGCACGCG
GGGAGGAGAAGGAGGGGTC
mm4__chr11 65.59 Mbp_chr11_65592407 GGGTGGCCCATAACTTCTCGCGTCCCCGGCGTCCGCCGCCGCCCCGGGCCTGCGCTGCGGACGACCCGGCCGTCCCGGAG
GCCGCGCGCGTGTCCGCTAGGGGCCGCCGGCGCCCTTCCCCGTACAGGGCAGGGCGGATC
mm4__chr21 29.30 Mbp_chr21_29299115 GACAGGTCCAGTGAAGGCGCGGGCGGGCCGGGGGCGGCTCTTTCTGGGCTGGGCGGGGCGCGGGGGGCACGTATCGGGGC
CCGCGGGGGGCGCCGGCGGCCGGGATCCTGGAAGTGGCCG
mm4__chr15 90.22 Mbp_chr15_90219724 TCCTAGACTTGAGTGCCCCATCCCCAGTCCTGGGAGCAGAGGCCGGGCCTGGGTGTGGAAGGGGGGGCTCGGCGGTGCCC
CCTGGTGGCATGCTTGGGCGTGGGACTGATATC
mm4__chr1 40.32 Mbp_chr1_40316743 GGAAGGGAGACGTGGGTAGGCGGGCAGGGCCGAGAGGCCGCCTCGGGGCGACAGCGGCGTCTGGTTGGAGCCGGCGCCCC
CTGGGAGGCGGCGGGGGCGGAGGCTGCGCAGCGGGTGAATGAGCACCATTGTATGCCCC
mm4__chr1 155.02 Mbp_chr1_155017649 GGTAAGGGGTTGGAAGAGCCTTGTGGAGAGCGGGCGAGCCGGCGCCATCTGGCGGCCATGCTCTGAGTGGGCGAGCGCCC
CCCGCGGCCACTGGAGCGAGCTGTCTTCACGCTCCTCAT
mm4__chr5 126.60 Mbp_chr5_126601342 CGAACTGGCTGCTCCCTGCGAGGCGGGGCGCGCGAGCCCTGGCGCCTCCTGGAGGCCGGCTTGCGGCGGCCTCTTAGACG
TCTCAC
mm4__chr19 50.31 Mbp_chr19_50314437 TGGGAAGGCTGGAGACCTTAAAGGCCAGCCCGACCCCCGGGAGGTGCCACCCCCTTCCCAGAGTGCCTGCCCCTCAAACC
CGCGCATGCGGCCCCTGGCGGCTTATTGCTGAGGTGGACTTGGAG
mm4__chr4 125.32 Mbp_chr4_125317217 CAGTTCTCACTACCAGGCGGGGGTGCCTGAGGACGCGGTTGTGGGTTCCAGCGTCCTCCAGGTGNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNATCCGCTATCGCCTGCAGGACGAG
mm4__chr1 2.60 Mbp_chr1_2603278 CCTTCTGCCCTTGCCCTCCCCCCACTGACTTGGGCACCCTTTGCCGAAGCTGCACGCCTGCGCCTCCTGGGGGAGGAGGC
CCTCGAGAAGGCCAGCTGCTCCAGCAGGGTGAGAGTCGGCTGAGGGAG
mm4__chr19 55.70 Mbp_chr19_55703889 TTGGAGGAAGCTGGAAAGCGCTCCCCTGCGTTTCGGCTGCTGGCCCAGCAGAGGGCGCCGGAGAGCGGACGGAGCCGCAG
GCGGGGTTGTGGGAAGACGCTGGGATCCCGTGCGCGCTTGTGCTCCTTCCTTCTGGG
mm4__chr13 113.45 Mbp_chr13_113445448 TGGAGTCCACACAGCCTCTAAAATCAGAGCCCTGGTGCCTGGCCATGCCAGGACAGGCTGTGGCAGGCGGATGCCCACCA
GGTGGGGCCGGCGAGGGCGCCCCTCCCAACGGCCACTTTAACACCCA
mm4__chr1 40.32 Mbp_chr1_40316651 GAGCTCGAAACCACACCCAGCACCCGACCGGGCCCAGTCTCTGCCCTACCCGCGCGCCCGCAGCCCCCGGCGGCCCTCGG
AAGGGAGACGTGGGTAGG
mm4__chr9 39.13 Mbp_chr9_39133052 CTGCGTACGCGAAGGACACAGCCGAGCGCGGGTGCCCGCTGGGGGCACCTCGGAGGGTCACCGCGTCGGGGCCACCGTGC
TGCACCACCGTCCACGCGGCGTCTGCTGAGCAGAAACGGGCAAAGGAGAGGCATCACT
mm4__chr2 101.39 Mbp_chr2_101387189 GAGAACATCCCGGGTGGCGCGGGGGGCGGCCTGGCCCGGGCGGCGCCCGCCCGGGGCCGCAGGTGCGCGTCCTCCTTGTG
GGCAGAGGCGGGGAAGCTTTGCTGCCAGATGCTGCCCGAATCCTCTAGCAG
mm4__chr2 176.16 Mbp_chr2_176157422 GGAAAGTGCAGGGATCCCAAGAATATCACCCGTCCAGGGGGGCCGCGCGGTGCCCCCGGCCCTCCACCCCCGGCCCCCGG
CGGGCGCGGGAGCGCGGCCGCAGGTAAATATTTTGGCA
mm4__chr1 24.93 Mbp_chr1_24929913 GTGCTTGGGTCTACGGGAATACGCATAACAGCGGCCGTCAGGGCGCCGGGCAGGCGGAGACGGCGCGGCTTCCCCCGGGG
GCGGCCGGCGCGGGCGCCTCCTCGGCCGCCGCTGCCGCGAGAAGCGGGAAAGCAGAA
mm4__chr11 2.88 Mbp_chr11_2884333 AAGCATTTCCCCTTGTCCCGCGGCCAAGCCCCCCGGGGCCGCCGCCGCGCTTAACCCCCTCCACGCCGTGCTCACGGCGC
CCGGCCGCGCCCGGGGAGGGGCTCCCGCGGCCGGGGGCGAAAACTGCGCTC
mm4__chr10 102.64 Mbp_chr10_102645000 CCAGTCCCACGTGCAGACGCACCTGCAGCAGCCCTCCACCGCCCGCGGGCACCTCCTGGTGGAGGCGGACGACGTGCGGG
CCTGGAGCTGCCCGCAGCACAACGCCTACCGCCTCTACCACT
mm4__chr4 3.77 Mbp_chr4_3767712 CCGTCGAGTTCTTCCTGTCGCGCCGGCGCCGGGCGCGCAGCAGCGTGTGCCGCCGCAAGGTGGCCCAGGCGCGCGAGAAG
CGCTTCACCTTTGTGCTGGCTGTGGTCAT
mm4__chr5 3.60 Mbp_chr5_3599487 ATGCTGGCCATCATCACCAAGATGACCCTCACGCAGGTCTCCACCTGGTTCGCCAACGCGCGCCGGCGCCTCAAGAAGGA
GAACAAGGTGACATGGGGAGCGCGCAGCAAGGACCAGGAAGAT
mm4__chr5 2.75 Mbp_chr5_2749513 GGCCCAGGTCATCTTGTTCTCCTTCTTGAGGCGCCGGCGCGCGTTGGCGAACCAGGTGGAGACCTGGGTGAGGGTCATCT
TGGTG
mm4__chr5 1.88 Mbp_chr5_1879632 TTCTCGTCTGCGCACTTGTTCCGCGGCGGCCACGTCATCTTGTTCTCCTTCTTGAGGCGCCGGCGCGCGTTGGCGAACCA
GGTGGAGACCTGTGTGAGGGTCATCTTGGTGATGATGGCCAGCAT
mm4__chr16 54.29 Mbp_chr16_54285318 GCTCCCATAAGCGTTTCCCTCCTCGTCAGTGCGGCTGCGAGGCGCCCAAGTCATCTTATTCTCCTTCTTGAGGCGCCGGC
GCGCGTTGGCGAACCAGGTGGACACCTGGGTGAGGGTCATCTTGGTG
mm3__chr17 64.13 Mbp_chr17_64129569 CTGGATCCCAACGCCCTTTCGGCTCCTCCGCGGCCCCCGCCAGGCAGCGCCGGCGCCCGCCCGCGAGGAGGAGGGTCCCG
CAGGTGGGCGGCCTCCTCAGGCCGGGGCCTTCTGGGACCCCCAAGTGCCGTGCAGGACG
mm4__chr19 44.91 Mbp_chr19_44908883 ATGACCTGCAGAAGCGCCTGGCAGTGTACCAGGCCGGGGCCCGCGAGGGCGCCGAGCGCGGCCTCAGCGCCATCCGCGAG
CGCCTGGGGCCCCTGGTGGAACAGGGCCGCGTGCGGGCCGCCACTGTGGGCTCCCTGG
mm3__chr7 100.57 Mbp_chr7_100573919 CTCCTGGCGAACCCACCCCAGAGGCCTCGCGGCGACGCTGGCGCCCTGTGCGTCTCCCGGCCACGTGGCGCGTGCCCCCG
CCGGGCTCCCGCGGGCGCCCCCTGGCGACTGGGCGGTGAATTCCGGAAACAGAGGCCC
mm4__chrX 118.35 Mbp_chrX_118346458 CAGATCGTCTGCTCCCTCAGCCTCGCCCGAGACCCACTTCCCCAGTCTCGCCCGGGTGGAGGTCGACGAGGAGGAGACAA
GAGTCACCCTTCCTCCAGGCGGCGCCGGCCCCCTCACCCGCGGGTGTGTCCTATAAA
mm4__chr12 57.77 Mbp_chr12_57772867 CCATCACGCAGAACTTTGGGTCCCGCCTCGGGGTGGCGGCGCGCGTGTGGGACGCGGTGAGGAATGGGCTGCGCCGGGTG
AGGGTCCGTGGGAGGTCCAGATCCCGGACTCCGCCTCTCCCATAT
mm4__chr7 51.32 Mbp_chr7_51317109 CTCTCTGGGCGGAGTGGGGGATCCCGGGGCCGGCGCCTCCTGGCGGAGTTGGGGGAGGGGAACCACCTGGAGCCTGGAGG
CCGTGCGAGGGCGAGGTGGGCGGACCAGCCCGGGAGGACTGACGTTGGAGGGTGAGAA
mm4__chr19 53.88 Mbp_chr19_53882296 CTTTGGCTCTTCCTCCCCACTCGCCCGCTCCCCCTGGCGGAGCCGGCGCGCCCGGGGTGCCGCTCCCTGCCTGGCGCGCT
CCGCACCTGGAGGTGCCTTGCCCCTCTCCTGCCCACCTCGGAATTTCCCTGTGGCT
mm4__chr12 93.44 Mbp_chr12_93441487 GAGAAGAGGGGCTTGGCTGCCGAGGGACCTTGTCGGGCCACAATCCCCACCAGGCGCCCGAGCCCCGCCGCGATTCCCGC
GCGCCCGCGGCCTCCGGCGGGGGGGTGGTCTCAGAAGCTATGGAGGCCC
mm4__chr2 231.40 Mbp_chr2_231395585 ATGCACTGTCTACTAGGCGCGCTTGGGGAGCCTAGGGGCCGTCTCGGAGAAATGGGGTGGTCCCCGCCAGGCGGCGGCGG
GGCGGGCCCTGGGCGCCGAAGGGGTTAAGGCACCTGG
mm4__chrX 71.62 Mbp_chrX_71616969 GAAGTCTGGGAGGGCGAAAAGCAGGCAGAGCCCCCAGACAGCCAGGCAGGTGAGGGTCACGCGGGCCGGGGGCCCCCGGC
GGTAGAGCTGGGTGGCATGAACTATGTTCAGGTAGCGGTCAAAGCTGATGCA
mm4__chr4 54.10 Mbp_chr4_54100011 GCCTCCCCGGGAAATTAACAAAACCTACACTTTGCAAAGTCTCCCCCATCCCCCTCCCCCGAGTTCTTCCCCGCCGCGCG
CCCGCCCCTCCTCGCGGCTTCTGTTGTGACTCGGGCAG
mm4__chr16 67.53 Mbp_chr16_67528981 CAGCCATGTGAGTGTCCCGCGCCGTTTCCGGGGCTGCAGGCCGGGCGGGGAGCAGGCGCGGAGGTTCTGGTGCCCCCCCG
CCGGCGCCGCCCGGTGGGTCCGGGATCTGCGGCTCTGTC
mm4__chr11 65.64 Mbp_chr11_65638091 CTGGAATCCCCGCTGCTGCGCTTCCCGCCGCAGACAGCTCCCGGCCGGCCCCGCCTCCCGGCATGGCCCCGCCTCCCAGC
ATGGCCCCGCCCCCAGGCGGAGCCGGCGCCGGGAGCGGTAGCGGGAGAGC
mm4__chr1 36.17 Mbp_chr1_36172530 CAGTGTGGCTGGAGAAGGAGGAGAAGGCCAAGGCGCTGCGGGAGAAGCAGCTCCAGGAGCGCCGGCGCCGGCTGGAGGAG
CAACGTCTTAAAGCCGAGCAACGC
mm4__chr6 82.37 Mbp_chr6_82365293 TGCGCAACCTCTTCCTTACCGGCAACCAGCTGGCCGTGCTCCCTGCCGGCGCCTTCGCCCGCCGGCCGCCGCTGGCGGAG
CTGGCCGCGCTCAACCTCAGCGGCAGC
mm4__chr16 69.57 Mbp_chr16_69565729 ACCAGGTCGTTGCAAATACCTTTTCCCTCCCCGGGGCCCCAGCGCGCGGCCACCTCCCAGCCTCCCCCCCTCCCACCCTG
GCAGCGGGGCCCTTTCCCGGCTCAGGAACAGCAGCA
mm4__chr2 180.98 Mbp_chr2_180980511 GTGAGGGGAGGTGTGGAGAGCGCGCACGGGAGCGCGCGGGAACAGCTCGCGCCCGCCGGGCGTCGGCGGCGCGCGCCCCT
CCGCCAGGCAAGCAGCGAGCAC
mm4__chrX 153.97 Mbp_chrX_153972472 CGACAGTGGGAGGCGACGCAGCCGAGCGCCGGCGCCCGGGGGTGGAGCCTCCGCCCGCTCCCAGGCTCCCAGGCTGCGGG
GCCCGCCCTCGGGCGCCACCGCCTCCGCCCGCGCCGCAGCAAAATGAGCCGGG
mm4__chr6 13.81 Mbp_chr6_13814244 ACTAGCAGGAGGAGGAGCAGCGGTGAGGCACGTGACACGCCGCCGCGGGCCGCCGGGGCGCGAGGGCGCAGCGCCCCCAG
ACCGTCGGAGAGCGCAGAGAGGCAGCGGCGTGCTGAGGTTTCGCTGCC
mm4__chr17 9.00 Mbp_chr17_9003318 TTTCTTGAAGGCCTCGGAGGGCAGCTGGAGCGGCGTAGAGCCTGCGCGGGGATGGCGGGGGTGGCCGCGGGCCGGGGCCG
TCTGGGGGCGGCCATCCCGGGCGGTGCGTGGAAAAGTAGGCACT
mm4__chr17 44.18 Mbp_chr17_44176806 GACCTTGCTCTCTTGTCCCCAGGTGCGCCAAGTTGCTGCTGGAAGCAGGAGCGACGGTGAACCTGGCAGCAGGCGAGAGC
CAGGAGACGCCCCTGCACGTGGCGGCGGCGCGCGGCCTGGAGCAACATGTGGCTCT
mm4__chr10 46.28 Mbp_chr10_46282969 GAGCCGCCTTCAAGGGATGCCCTCGCCCTCCCGCAGGCGCCGGCGCCCGGCCCAGCCCGAGCCATCAGAGGGCTGTCCGC
CGAGCTGAGCCCGGGGAGGGCTGGGCTGCCCAGCTGATCAGAGGCTTT
mm4__chr10 46.11 Mbp_chr10_46106491 CGAGAGGTAGGGGAGCCTAGAGCCAGAGGGCGGCGGCGAGAGCTGGAGCCGCCTTCAAAGGACGCCCTCGCCCTCCCGCA
GGCGCCGGCGCCCGGCCCAGTCCGAGCCATCAGAGGGCTGTCTGC
mm2__chr2 26.85 Mbp_chr2_26847940 GCCTGCCGGGAGTTGTAGTCCCCANNNNNNNNNNNNNNNNNNNNCCAAGGATGCGGGACGCCGCCTGTCCCCGCCTCCCC
TTAACCTGCTAGC
mm4__chrX 40.10 Mbp_chrX_40095751 TCTTCGCTCTTCCATTCCCCCCACCCCCACCCTCCAGACGCCGGCGGCGCGCGCCCTGGCGCGCGGGCACTCACAGCCTC
TAGCTGGAGATCGCGGACTTTCTGGAAAAGATTCATTAACAGCAAATTACGCCTCGC
mm4__chr12 109.71 Mbp_chr12_109713546 CTGGTTTTGCCACCCCTAGTGGGACTACAAAAACCAGCACGCCCTGCGCCCCCAGTCGGGGCCCCTCCACCCGGCGCCGG
CGCGCAGCATGCCGGGAGTTGCAGTCCTCGCGAG
mm4__chr20 63.74 Mbp_chr20_63739513 CTTCCCCGGCTCTGGCAGCTCTGCGAGCGCGCCCGTGGGGAACGGGCCCTCCCCGGCGGGGCGCGCGGGCGCGCGAGGTG
GGCGGAGGCCTCGGAGCTGTGCCGGGCCGGGCCTCCCTCCCTAGGCCAG
mm4__chr20 1.89 Mbp_chr20_1894757 CTTCCCGGGACAGCAGCCGGGGCCCGGGCGCCGGCGCGGGGGAGGACGCTGCCCTCCCCGGCCGGGCGCGCAGCTCGCAG
CCCTCGCTCCCTCCCCCGGGGCGCGCAGGCGGGCGCTCGCTCGCAGAGAAGGTG
mm4__chr17 28.73 Mbp_chr17_28726022 GTAGGGGCAGGCGACAGAGCGCGCGCCGGAGCGCCCGGAAGGTCAGCGTGGCGCCCAGGAGCAGCGGCAGGGCGGGGTGC
AGCAGTCGGGGCATGCTGGCCCTCCCTGCCGTCCGCCCTCGAGGCCGCCTCCTAGGTCTG
mm4__chr1 34.98 Mbp_chr1_34976766 AACCCCAGGACCATGGTCTTGGCCGCGCGCCGGTGCGCGCCGGCGGTTGCAGGGCCCGGGCCCGGGCCAGAGGGCGCCTT
GAGCGCCCGCGCCAGGCTCACGTTGGCCGCGAGCACCAGCAGGAAGG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

Version 4.7 - Documentation  - Contact us - Downloads/local installation - Paper - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.