CRISPOR

Micro-homology scoring of potential deletions

Guide: CCCCACGATGGAGGGGAAGA CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	(Wild-type sequence)
397.6	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGG--------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`14 bp deleted → frameshift`
377.6	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGG---------------GCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`15 bp deleted → no frameshift`
359.2	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGG----------------CATCGTCGCCCGCGAAGCCGGCCTTGCA`	`16 bp deleted → frameshift`
313.2	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGG-------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`13 bp deleted → frameshift`
282.0	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGG-------CCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`7 bp deleted → frameshift`
268.0	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGG--------CCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`8 bp deleted → frameshift`
256.2	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGG-----------------CATCGTCGCCCGCGAAGCCGGCCTTGCA`	`17 bp deleted → frameshift`
255.2	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGG---------CCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`9 bp deleted → no frameshift`
253.6	`CCTACCTGGTGCCTGGGGCGCCC-----------------------GGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`23 bp deleted → frameshift`
219.6	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGG------------GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`12 bp deleted → no frameshift`
219.6	`CCTACCTGGTGCCTGGGGCGCCCCACGATGG------------CCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`12 bp deleted → no frameshift`
199.8	`CCTACCTGGTGCCTGGGGCGCCCC----------------------GGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`22 bp deleted → frameshift`
188.8	`CCTACCTGGTGCCTGGGGCGCCCCACG---------------GCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`15 bp deleted → no frameshift`
183.0	`CCTACCTGGTGCCTGGGGC----------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCA`	`34 bp deleted → frameshift`
172.2	`CCTACCTGGTGCCTGGGGC-------------------------CCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`25 bp deleted → frameshift`
170.8	`CCTACCTGGTGCCTGGGGCGCCCCACGATGG-----------------GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`17 bp deleted → frameshift`
162.8	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGG------------------CATCGTCGCCCGCGAAGCCGGCCTTGCA`	`18 bp deleted → no frameshift`
162.8	`CCTACCTGGTGCCTGGGGCGCCCCACGATGG------------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`18 bp deleted → no frameshift`
161.6	`CCTACCTGGTGCCTGGGG--------------------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`32 bp deleted → frameshift`
154.8	`CCTACCTGGTGCCTGGGGCGCCCCACGATGG-------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`19 bp deleted → frameshift`
153.6	`CCTACCTGGTGCCTGGGG---------------------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`33 bp deleted → no frameshift`
147.2	`CCTACCTGGTGCCTGGGGCGCCCCACGATGG--------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`20 bp deleted → frameshift`
147.2	`CCTACCTGGTGCCTGGGGCGCCCCACG--------------------GGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`20 bp deleted → frameshift`
140.0	`CCTACCTGGTGCCTGGGGCGCCCCACGATGG---------------------CATCGTCGCCCGCGAAGCCGGCCTTGCA`	`21 bp deleted → no frameshift`
140.0	`CCTACCTGGTGCCTGGGGCGCCCC---------------------CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`21 bp deleted → no frameshift`
135.0	`CCTACCTGGTGCCTGGGGCGCCC----------------------------------------GCGAAGCCGGCCTTGCA`	`40 bp deleted → frameshift`
133.8	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAG------------------------------CCGGCCTTGCA`	`30 bp deleted → no frameshift`
133.2	`CCTACCTGGTGCCTGGGGCG----------------------GCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`22 bp deleted → frameshift`
127.2	`CCTACCTGGTGCCTGGGG-------------------------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`31 bp deleted → frameshift`
121.2	`CCTACCTGGTGCC--------------------------------CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`32 bp deleted → frameshift`
120.4	`CCTACCTGGTGCCTGGGGCGCC------------------------GGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`24 bp deleted → no frameshift`
109.2	`CCTACCTGGTGCCTGGG--------------------------CCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`26 bp deleted → frameshift`
104.4	`CCTACCTGGTGCCTGGG-----------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCA`	`35 bp deleted → frameshift`
103.6	`CCTACCTGGTGCCTGGGGCG---------------------------GGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`27 bp deleted → no frameshift`
103.6	`CCTACCTGGTGCCTGG---------------------------CCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`27 bp deleted → no frameshift`
89.2	`CCTACCTGGTGCCTGGGGCGCCCCACG------------------------------TCGCCCGCGAAGCCGGCCTTGCA`	`30 bp deleted → no frameshift`
89.2	`CCTACCTGGTGCCTGGGG------------------------------GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`30 bp deleted → no frameshift`
85.2	`CCTACCTGGTGCCTGGGGCGCCCC---------------------------------------GCGAAGCCGGCCTTGCA`	`39 bp deleted → no frameshift`
80.8	`CCTACCTGGTGCCTGGGGCGC--------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCA`	`32 bp deleted → frameshift`
77.7	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGA---------------------------AGCCGGCCTTGCA`	`27 bp deleted → no frameshift`
76.8	`CCTACCTGGTGCCTGGGGCGCCCCACG---------------------------------CCCGCGAAGCCGGCCTTGCA`	`33 bp deleted → no frameshift`
76.8	`CCTACCTGGTGCC---------------------------------GGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`33 bp deleted → no frameshift`
73.2	`CCTACCTGG----------------------------------CCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`34 bp deleted → frameshift`
71.0	`CCTACCTGGTGCCTGGGGCGCCCCACGA---------------------------------------AGCCGGCCTTGCA`	`39 bp deleted → no frameshift`
70.5	`CCTACCTGGTGCCTGGGGCGCCCCA-----------------------------TCGTCGCCCGCGAAGCCGGCCTTGCA`	`29 bp deleted → frameshift`
66.6	`CCTACCTGGTGCCTGGGGCGC--------------------------------------------GAAGCCGGCCTTGCA`	`44 bp deleted → frameshift`
66.0	`CCTACCTGGTGCCTGG------------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCA`	`36 bp deleted → no frameshift`
62.8	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGG-------------------------------------CCTTGCA`	`37 bp deleted → frameshift`
62.8	`CCTACCTGGTGCCTGGGGCGCCCCACG-------------------------------------CGAAGCCGGCCTTGCA`	`37 bp deleted → frameshift`
62.8	`CCTACCTGGTGCCTGGGGCG-------------------------------------TCGCCCGCGAAGCCGGCCTTGCA`	`37 bp deleted → frameshift`
60.0	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGG--------------------------------------CCTTGCA`	`38 bp deleted → frameshift`
60.0	`CCTACCTGGTGCCTGGGGCGCCCC--------------------------------------CGCGAAGCCGGCCTTGCA`	`38 bp deleted → frameshift`
60.0	`CCTACCTGGTGCCTGGGGCG----------------------------------------------AAGCCGGCCTTGCA`	`46 bp deleted → frameshift`
56.8	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAGG---------------------------------------CCTTGCA`	`39 bp deleted → no frameshift`
56.8	`CCTACCTGG---------------------------------------GGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`39 bp deleted → no frameshift`
56.8	`CCTACC---------------------------------------CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`39 bp deleted → no frameshift`
54.6	`CCTACCTGGTGCCTGGGGCGCCCCACGAT--------------------------CGTCGCCCGCGAAGCCGGCCTTGCA`	`26 bp deleted → frameshift`
54.0	`CCTACCTGG----------------------------------------GGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`40 bp deleted → frameshift`
54.0	`CCTACC----------------------------------------GGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`40 bp deleted → frameshift`
52.2	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGA-----------------------------------AGCCGGCCTTGCA`	`35 bp deleted → frameshift`
51.6	`CCTACCTGGTGCCTGGGGCGCC-----------------------------------------GCGAAGCCGGCCTTGCA`	`41 bp deleted → frameshift`
51.6	`CCTACCTGGTGC-----------------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCA`	`41 bp deleted → frameshift`
51.6	`CCTACCTGG-----------------------------------------GGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`41 bp deleted → frameshift`
51.6	`CCTACCTGGTGCCTGGGGCGCC-------------------------------------------------GGCCTTGCA`	`49 bp deleted → frameshift`
51.6	`CCTACCTGGTGCC-------------------------------------------------CGCGAAGCCGGCCTTGCA`	`49 bp deleted → frameshift`
49.5	`CCTACCTGGTGCCTGGGGCGCCCCACGATGGAG------------------------------------CCGGCCTTGCA`	`36 bp deleted → no frameshift`
48.8	`CCTACCTGGTGCCTGGGGCGCCCCACGATGG------------------------------------------CCTTGCA`	`42 bp deleted → no frameshift`
48.8	`CCTACCTGGTGCCTGGGGC------------------------------------------CCGCGAAGCCGGCCTTGCA`	`42 bp deleted → no frameshift`
48.8	`CCTACCTGG------------------------------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`42 bp deleted → no frameshift`
46.4	`CCTACCTGG-------------------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCA`	`43 bp deleted → frameshift`
46.4	`CC-------------------------------------------CGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`43 bp deleted → frameshift`
44.4	`CC--------------------------------------------GGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCA`	`44 bp deleted → frameshift`
42.6	`CCTACCTGGTGCCTGGGGCGCC-----------------------------------------------------TTGCA`	`53 bp deleted → frameshift`
42.0	`CCTACCTGGTGCCTGGGGCGCCCCACG---------------------------------------------GCCTTGCA`	`45 bp deleted → no frameshift`
38.4	`CCTACCTGGTGCCTGGGGC-------------------------------------------------------CTTGCA`	`55 bp deleted → frameshift`
38.0	`CCTACCTGGTGCCTGGGGCGCCCC-----------------------------------------------GGCCTTGCA`	`47 bp deleted → frameshift`
36.4	`CCTACCTGGTGCCTGGGGCGCCC------------------------------------------------GGCCTTGCA`	`48 bp deleted → no frameshift`
33.0	`CCTACCTGGTGCC----------------------------------------------------------GGCCTTGCA`	`58 bp deleted → frameshift`
32.8	`CCTACCTGGTGCC--------------------------------------------------GCGAAGCCGGCCTTGCA`	`50 bp deleted → frameshift`
31.5	`CCTACCTGGTGCCT--------------------------------------------------------------TGCA`	`62 bp deleted → frameshift`
31.2	`CCTACCTGGTGCCTGGGGCGCCCC---------------------------------------------------TTGCA`	`51 bp deleted → no frameshift`
31.2	`CCTACCTGGTGCCTGGGGC---------------------------------------------------CGGCCTTGCA`	`51 bp deleted → no frameshift`
29.6	`CCTACCTGGTGCCTGGGGCGCCC----------------------------------------------------TTGCA`	`52 bp deleted → frameshift`
29.6	`CCTACCTGGTGCCTGGGGCG----------------------------------------------------GCCTTGCA`	`52 bp deleted → frameshift`
28.4	`CCTACCTGGTGC-----------------------------------------------------GAAGCCGGCCTTGCA`	`53 bp deleted → frameshift`
27.3	`CCTACCTGGTGCCTGGGGCGCCCCACGATG------------------------------------------------CA`	`48 bp deleted → no frameshift`
27.3	`CCTACCTGGT------------------------------------------------CGCCCGCGAAGCCGGCCTTGCA`	`48 bp deleted → no frameshift`
24.4	`CCTACCTGGTGCCTGGG--------------------------------------------------------CCTTGCA`	`56 bp deleted → frameshift`
24.4	`CCTACC--------------------------------------------------------CGCGAAGCCGGCCTTGCA`	`56 bp deleted → frameshift`
23.2	`CCTACCTGGTGCCTGG---------------------------------------------------------CCTTGCA`	`57 bp deleted → no frameshift`
23.2	`CCTACC---------------------------------------------------------GCGAAGCCGGCCTTGCA`	`57 bp deleted → no frameshift`
22.0	`CCTACCTGGTGCCTGGGGCGC----------------------------------------------------------A`	`58 bp deleted → frameshift`
20.0	`CCTACCTGGTGCCTGGGGC------------------------------------------------------------A`	`60 bp deleted → no frameshift`
20.0	`CC------------------------------------------------------------CGCGAAGCCGGCCTTGCA`	`60 bp deleted → no frameshift`
19.2	`CCTACCTGGTGCCTGGGGCGCCCCA-------------------------------------------------------`	`55 bp deleted → frameshift`
18.8	`CC-------------------------------------------------------------GCGAAGCCGGCCTTGCA`	`61 bp deleted → frameshift`
17.5	`CCTACCTGGTGC-------------------------------------------------------------------A`	`67 bp deleted → frameshift`
16.4	`CCTACCTGG----------------------------------------------------------------CCTTGCA`	`64 bp deleted → frameshift`
16.0	`CCTACCT---------------------------------------------------------------------TGCA`	`69 bp deleted → no frameshift`
15.6	`CCTACC-----------------------------------------------------------------GGCCTTGCA`	`65 bp deleted → frameshift`
13.0	`CCT-------------------------------------------------------------------------TGCA`	`73 bp deleted → frameshift`
12.9	`CCTACCTGGTGCCTG---------------------------------------------------------------CA`	`63 bp deleted → no frameshift`
12.8	`CC---------------------------------------------------------------------GGCCTTGCA`	`69 bp deleted → no frameshift`
9.0	`CCTACCTG----------------------------------------------------------------------CA`	`70 bp deleted → frameshift`

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