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Contents:

PCR primers for off-targets of ATGGAGGGGAAGACGGCCCG GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529556_F TCGTCGGCAGCGTCCTCCCCTACCTGGTGCCT 59.9 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529556_R GTCTCGTGGGCTCGGTGGATGATGATATCGCCGCG 60.4 1.00
mm0__chr5 131.66 Mbp_chr5_131660015_F TCGTCGGCAGCGTCATGCCCATCATCACACCCTG 60.1 1.00
mm0__chr5 131.66 Mbp_chr5_131660015_R GTCTCGTGGGCTCGGTGTGGAAGGTCTGCTTCACG 60.2 1.00
mm2__chr22 15.72 Mbp_chr22_15721564_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.94
mm2__chr22 15.72 Mbp_chr22_15721564_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.94
mm2__chr2 131.26 Mbp_chr2_131263633_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.94
mm2__chr2 131.26 Mbp_chr2_131263633_R GTCTCGTGGGCTCGGCTCTTTCTGATGCATGCCCC 58.9 0.94
mm2__chr2 130.66 Mbp_chr2_130656827_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.94
mm2__chr2 130.66 Mbp_chr2_130656827_R GTCTCGTGGGCTCGGCTCTTTCTGATGCATGCCCC 58.9 0.94
mm2__chr2 130.46 Mbp_chr2_130463842_F TCGTCGGCAGCGTCCTCTTTCTGATGCATGCCCC 58.9 0.94
mm2__chr2 130.46 Mbp_chr2_130463842_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.94
mm2__chr2 130.08 Mbp_chr2_130075270_F TCGTCGGCAGCGTCCTCTTTCTGATGCATGCCCC 58.9 0.94
mm2__chr2 130.08 Mbp_chr2_130075270_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.94
mm2__chr14 19.40 Mbp_chr14_19402807_F TCGTCGGCAGCGTCATCATGTTCTGGTGCCTGGG 60.0 0.94
mm2__chr14 19.40 Mbp_chr14_19402807_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.94
mm2__chr14 19.00 Mbp_chr14_18998945_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.94
mm2__chr14 19.00 Mbp_chr14_18998945_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.94
mm1__chr2 73.90 Mbp_chr2_73901392_F TCGTCGGCAGCGTCAGGCTTCGCAGGAGATGATG 59.8 0.94
mm1__chr2 73.90 Mbp_chr2_73901392_R GTCTCGTGGGCTCGGTATCCAGATGAGCACGCACC 59.8 0.94
mm1__chr11 1.80 Mbp_chr11_1803994_F TCGTCGGCAGCGTCCGCCACCCACATAGAAGTCC 60.4 0.93
mm1__chr11 1.80 Mbp_chr11_1803994_R GTCTCGTGGGCTCGGAACACCTCTGGCATGTGCAA 60.4 0.93
mm1__chr19 9.52 Mbp_chr19_9518227_F TCGTCGGCAGCGTCAGTTAACAATGGCTCCGGCA 59.9 0.73
mm1__chr19 9.52 Mbp_chr19_9518227_R GTCTCGTGGGCTCGGGGAGTCCTTCTGACCGATGC 60.1 0.73
mm3__chr22 41.08 Mbp_chr22_41075138_F TCGTCGGCAGCGTCGGAGTCCTTCTGACCCATGC 60.1 0.68
mm3__chr22 41.08 Mbp_chr22_41075138_R GTCTCGTGGGCTCGGTGTTGACAACAGCTCTGGCA 60.1 0.68
mm3__chr1 223.86 Mbp_chr1_223864750_F TCGTCGGCAGCGTCTGACCCATGCTCACCATCAC 60.0 0.68
mm3__chr1 223.86 Mbp_chr1_223864750_R GTCTCGTGGGCTCGGCGCTCGTCATTGACAACAGC 60.1 0.68
mm2__chr15 43.99 Mbp_chr15_43990081_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 0.66
mm2__chr15 43.99 Mbp_chr15_43990081_R GTCTCGTGGGCTCGGTGTCGTCGACAATGGCTCTG 60.3 0.66
mm3__chr5 80.30 Mbp_chr5_80302746_F TCGTCGGCAGCGTCCTCTTGCTCTGGGCCTCATC 60.1 0.55
mm3__chr5 80.30 Mbp_chr5_80302746_R GTCTCGTGGGCTCGGTGGCATATGCAAGGCTAGCT 59.5 0.55
mm2__chr5 57.48 Mbp_chr5_57482603_F TCGTCGGCAGCGTCATCTCCCACGTAGCAGTCCT 60.0 0.49
mm2__chr5 57.48 Mbp_chr5_57482603_R GTCTCGTGGGCTCGGATGGGTCAGGGATGTGCAAG 60.0 0.49
mm3__chr5 98.34 Mbp_chr5_98338820_F TCGTCGGCAGCGTCGCTCCTCATCAACAAGGGCT 60.0 0.47
mm3__chr5 98.34 Mbp_chr5_98338820_R GTCTCGTGGGCTCGGTTTTGACCCATGCCCACCAT 60.1 0.47
mm1__chr6 88.28 Mbp_chr6_88276906_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 0.46
mm1__chr6 88.28 Mbp_chr6_88276906_R GTCTCGTGGGCTCGGTGGATGATGCTATCACCGCG 60.3 0.46
mm3__chr9 137.27 Mbp_chr9_137271778_F Primer3: not found at this Tm N.d. 0.43
mm3__chr9 137.27 Mbp_chr9_137271778_R Primer3: not found at this Tm N.d. 0.43
mm4__chr8 119.21 Mbp_chr8_119208667_F Primer3: not found at this Tm N.d. 0.42
mm4__chr8 119.21 Mbp_chr8_119208667_R Primer3: not found at this Tm N.d. 0.42
mm4__chr9 137.27 Mbp_chr9_137271816_F Primer3: not found at this Tm N.d. 0.41
mm4__chr9 137.27 Mbp_chr9_137271816_R Primer3: not found at this Tm N.d. 0.41
mm4__chr10 88.95 Mbp_chr10_88948823_F TCGTCGGCAGCGTCCTCACCTGATGTCTGGGACG 59.8 0.41
mm4__chr10 88.95 Mbp_chr10_88948823_R GTCTCGTGGGCTCGGACTGCCTTGGTGTGTGACAA 60.0 0.41
mm4__chr2 131.63 Mbp_chr2_131626216_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.32
mm4__chr2 131.63 Mbp_chr2_131626216_R GTCTCGTGGGCTCGGACTCCTTCTGATGCATGCCC 60.1 0.32
mm3__chr3 12.07 Mbp_chr3_12070302_F TCGTCGGCAGCGTCTCATTGACAGTGTCTCCGGC 60.0 0.31
mm3__chr3 12.07 Mbp_chr3_12070302_R GTCTCGTGGGCTCGGGAGTCCTTCTGACCCATGCC 60.1 0.31
mm3__chr17 44.26 Mbp_chr17_44262508_F TCGTCGGCAGCGTCCACTGGATGGGTGACTGCC 60.3 0.30
mm3__chr17 44.26 Mbp_chr17_44262508_R GTCTCGTGGGCTCGGTTCTCCACAGTCCTCCTCCC 60.2 0.30
mm4__chrX 53.14 Mbp_chrX_53143811_F TCGTCGGCAGCGTCCATGGCCCATGTAGGAGTCC 59.8 0.30
mm4__chrX 53.14 Mbp_chrX_53143811_R GTCTCGTGGGCTCGGCTCTGGCATGTGCAAAGCTG 60.1 0.30
mm3__chr2 239.39 Mbp_chr2_239392592_F TCGTCGGCAGCGTCCAAGCAAGGAGGCACAGTTG 59.6 0.28
mm3__chr2 239.39 Mbp_chr2_239392592_R GTCTCGTGGGCTCGGGTTCTAAGGAGGCTGACCCC 59.4 0.28
mm3__chr10 69.02 Mbp_chr10_69022831_F Primer3: not found at this Tm N.d. 0.28
mm3__chr10 69.02 Mbp_chr10_69022831_R Primer3: not found at this Tm N.d. 0.28
mm4__chr2 43.16 Mbp_chr2_43158724_F TCGTCGGCAGCGTCAGGCCAGGGAGGAGAGTTAG 60.0 0.28
mm4__chr2 43.16 Mbp_chr2_43158724_R GTCTCGTGGGCTCGGCAAGCAGCACCGGTCTCTTT 60.8 0.28
mm4__chr7 98.82 Mbp_chr7_98822872_F TCGTCGGCAGCGTCGCCAAGGCCCCTTAGATCTT 59.4 0.28
mm4__chr7 98.82 Mbp_chr7_98822872_R GTCTCGTGGGCTCGGGCCCCTTACACAGGCTAGTC 59.8 0.28
mm3__chr20 63.31 Mbp_chr20_63305080_F TCGTCGGCAGCGTCATAGGGTAGGAGCCTGTGGG 60.1 0.25
mm3__chr20 63.31 Mbp_chr20_63305080_R GTCTCGTGGGCTCGGGTATGAAGACAGGGCTGCCC 60.4 0.25
mm3__chr1 229.43 Mbp_chr1_229433008_F TCGTCGGCAGCGTCGCAGCCTGACCTGGTGTC 60.3 0.25
mm3__chr1 229.43 Mbp_chr1_229433008_R GTCTCGTGGGCTCGGGACGAAGACGAGACCACCG 60.1 0.25
mm4__chr7 100.89 Mbp_chr7_100890359_F TCGTCGGCAGCGTCCACCATGTAGGAGCTCCAGC 60.1 0.25
mm4__chr7 100.89 Mbp_chr7_100890359_R GTCTCGTGGGCTCGGCAAATGTCCGATCCCCGACC 60.8 0.25
mm3__chr1 202.87 Mbp_chr1_202873369_F TCGTCGGCAGCGTCTGACAATGGCTCCAGCACAT 59.9 0.25
mm3__chr1 202.87 Mbp_chr1_202873369_R GTCTCGTGGGCTCGGGGAGTCCTTCTGGCCTATGC 59.8 0.25
mm4__chr17 81.51 Mbp_chr17_81512253_F TCGTCGGCAGCGTCCCATCCACTCACCTGGTGTC 60.0 0.21
mm4__chr17 81.51 Mbp_chr17_81512253_R GTCTCGTGGGCTCGGATGGAAGAAGAGATCGCCGC 60.2 0.21
mm3__chr17 31.59 Mbp_chr17_31586520_F Primer3: not found at this Tm N.d. 0.21
mm3__chr17 31.59 Mbp_chr17_31586520_R Primer3: not found at this Tm N.d. 0.21
mm4__chr13 29.17 Mbp_chr13_29165232_F Primer3: not found at this Tm N.d. 0.21
mm4__chr13 29.17 Mbp_chr13_29165232_R Primer3: not found at this Tm N.d. 0.21
mm4__chr20 51.59 Mbp_chr20_51586257_F TCGTCGGCAGCGTCGTGGCAATTCCCTGGGAAGT 60.2 0.20
mm4__chr20 51.59 Mbp_chr20_51586257_R GTCTCGTGGGCTCGGGGCATGTCACAGGAGGTTGA 59.9 0.20
mm4__chr2 46.98 Mbp_chr2_46983266_F Primer3: not found at this Tm N.d. 0.20
mm4__chr2 46.98 Mbp_chr2_46983266_R Primer3: not found at this Tm N.d. 0.20
mm4__chr8 100.73 Mbp_chr8_100725167_F TCGTCGGCAGCGTCCCTGATGGTCACATGGGCA 59.6 0.19
mm4__chr8 100.73 Mbp_chr8_100725167_R GTCTCGTGGGCTCGGAAATGAGAGCCCAACCAGCA 59.8 0.19
mm4__chr11 789.27 Kbp_chr11_789273_F TCGTCGGCAGCGTCCTCTCCAGGAAGTCAGCGTG 60.1 0.19
mm4__chr11 789.27 Kbp_chr11_789273_R GTCTCGTGGGCTCGGCGAGGACTCCCTGCTCCA 60.3 0.19
mm4__chr6 30.26 Mbp_chr6_30260823_F TCGTCGGCAGCGTCTAACTCTGAGAGCCCACCCT 59.5 0.18
mm4__chr6 30.26 Mbp_chr6_30260823_R GTCTCGTGGGCTCGGGGAAAATTCCTGGTGCCCCA 60.5 0.18
mm3__chr6 31.27 Mbp_chr6_31270853_F TCGTCGGCAGCGTCCTGCTGCAGTGGTCAAAGTG 59.6 0.18
mm3__chr6 31.27 Mbp_chr6_31270853_R GTCTCGTGGGCTCGGCTGAGTTTCCTCTGAGGGCC 59.7 0.18
mm4__chr21 43.27 Mbp_chr21_43267526_F TCGTCGGCAGCGTCCTCCAAGAAGCCCTGCAGTC 60.6 0.17
mm4__chr21 43.27 Mbp_chr21_43267526_R GTCTCGTGGGCTCGGTATCAGGCCGGCCTTTTCAG 60.1 0.17
mm4__chr5 17.23 Mbp_chr5_17225397_F TCGTCGGCAGCGTCCACTACCTGCTGCCACTTCA 59.9 0.16
mm4__chr5 17.23 Mbp_chr5_17225397_R GTCTCGTGGGCTCGGAAAGGAAACCACGTGCACAT 58.6 0.16
mm4__chr19 48.20 Mbp_chr19_48196453_F TCGTCGGCAGCGTCGTATTGGAAGGCAGCCCAGA 59.7 0.16
mm4__chr19 48.20 Mbp_chr19_48196453_R GTCTCGTGGGCTCGGCAGTCCACCTCCTCCTCTCC 60.6 0.16
mm4__chr8 84.95 Mbp_chr8_84948666_F TCGTCGGCAGCGTCCTCTGGTATGTGCAAGGCCA 60.0 0.16
mm4__chr8 84.95 Mbp_chr8_84948666_R GTCTCGTGGGCTCGGCAGTCCTTCTGCCCTATGCC 60.1 0.16
mm4__chr1 81.80 Mbp_chr1_81801131_F TCGTCGGCAGCGTCTATCCCCTTTCGCTCCTCCT 59.7 0.16
mm4__chr1 81.80 Mbp_chr1_81801131_R GTCTCGTGGGCTCGGGGCAGCTAAGTACAAAACGTGG 60.0 0.16
mm4__chr15 24.95 Mbp_chr15_24947265_F TCGTCGGCAGCGTCAGAGACCCTGCCACAAAAGT 59.1 0.15
mm4__chr15 24.95 Mbp_chr15_24947265_R GTCTCGTGGGCTCGGTCCCTGTATAACTGCCACATCA 58.8 0.15
mm4__chr3 123.39 Mbp_chr3_123393764_F Primer3: not found at this Tm N.d. 0.15
mm4__chr3 123.39 Mbp_chr3_123393764_R Primer3: not found at this Tm N.d. 0.15
mm4__chr14 95.85 Mbp_chr14_95850047_F TCGTCGGCAGCGTCGCTAGAACTCTAGTCCCTCCCA 60.0 0.15
mm4__chr14 95.85 Mbp_chr14_95850047_R GTCTCGTGGGCTCGGCAGCATTTGAGCTTGGCCTG 60.1 0.15
mm4__chr4 60.02 Mbp_chr4_60020983_F Primer3: not found at this Tm N.d. 0.15
mm4__chr4 60.02 Mbp_chr4_60020983_R Primer3: not found at this Tm N.d. 0.15
mm4__chr3 45.13 Mbp_chr3_45128222_F TCGTCGGCAGCGTCGACCTGGGGAGTGATCAAGC 60.1 0.14
mm4__chr3 45.13 Mbp_chr3_45128222_R GTCTCGTGGGCTCGGGTGCATGCCCTTGTCCTACT 60.0 0.14
mm4__chr10 101.03 Mbp_chr10_101031717_F Primer3: not found at this Tm N.d. 0.14
mm4__chr10 101.03 Mbp_chr10_101031717_R Primer3: not found at this Tm N.d. 0.14
mm4__chr7 43.13 Mbp_chr7_43131946_F TCGTCGGCAGCGTCTGGCACAAGTGTTCACCCTT 60.0 0.14
mm4__chr7 43.13 Mbp_chr7_43131946_R GTCTCGTGGGCTCGGTCGATGCCCTTGTAACCCAG 59.7 0.14
mm4__chr20 61.06 Mbp_chr20_61057786_F TCGTCGGCAGCGTCTGAACGACACTCCAGGAAGC 59.9 0.14
mm4__chr20 61.06 Mbp_chr20_61057786_R GTCTCGTGGGCTCGGGATCGACTCCCCAGGAAAGC 60.1 0.14
mm4__chr19 13.15 Mbp_chr19_13149864_F Primer3: not found at this Tm N.d. 0.14
mm4__chr19 13.15 Mbp_chr19_13149864_R Primer3: not found at this Tm N.d. 0.14
mm4__chr17 58.50 Mbp_chr17_58504251_F TCGTCGGCAGCGTCCAGAGAGAGCTTGCACCTGG 60.3 0.13
mm4__chr17 58.50 Mbp_chr17_58504251_R GTCTCGTGGGCTCGGCAGTTCCCCTGCCTGTTTGA 60.1 0.13
mm4__chr7 43.73 Mbp_chr7_43729870_F TCGTCGGCAGCGTCTTGCTCCTCACAGGTATGCG 60.1 0.12
mm4__chr7 43.73 Mbp_chr7_43729870_R GTCTCGTGGGCTCGGGCTGCCAAATTTGTCAGCTGA 60.0 0.12
mm4__chr17 49.00 Mbp_chr17_48997575_F TCGTCGGCAGCGTCGCACTTCTCCTGGGCTCTC 59.7 0.12
mm4__chr17 49.00 Mbp_chr17_48997575_R GTCTCGTGGGCTCGGACTTTCTTTGCACCCCACCC 60.7 0.12
mm2__chr18 62.44 Mbp_chr18_62442086_F TCGTCGGCAGCGTCCCTCGTCATCGACAATGGCT 60.1 0.12
mm2__chr18 62.44 Mbp_chr18_62442086_R GTCTCGTGGGCTCGGGAGTCCTTCTGACCCATGCC 60.1 0.12
mm2__chr5 54.95 Mbp_chr5_54950600_F Primer3: not found at this Tm N.d. 0.11
mm2__chr5 54.95 Mbp_chr5_54950600_R Primer3: not found at this Tm N.d. 0.11
mm4__chr6 101.43 Mbp_chr6_101431436_F TCGTCGGCAGCGTCTGATGCTGTCCTGTGTGCTT 59.8 0.11
mm4__chr6 101.43 Mbp_chr6_101431436_R GTCTCGTGGGCTCGGCGGCTTTGCTGGGGATGA 60.0 0.11
mm2__chr9 6.83 Mbp_chr9_6834532_F TCGTCGGCAGCGTCTGATATTGCTACGCCCGTCG 60.3 0.11
mm2__chr9 6.83 Mbp_chr9_6834532_R GTCTCGTGGGCTCGGACCCATGCCCACCATCATG 60.0 0.11
mm4__chr1 6.27 Mbp_chr1_6270858_F TCGTCGGCAGCGTCATACCTGAGGCCAAGTGCAG 59.7 0.11
mm4__chr1 6.27 Mbp_chr1_6270858_R GTCTCGTGGGCTCGGTGTGACATCCTAAAGGGCCC 59.3 0.11
mm4__chr17 7.19 Mbp_chr17_7192396_F Primer3: not found at this Tm N.d. 0.10
mm4__chr17 7.19 Mbp_chr17_7192396_R Primer3: not found at this Tm N.d. 0.10
mm4__chr9 126.03 Mbp_chr9_126028191_F TCGTCGGCAGCGTCACAGAGCTCTCATCTCCCGA 59.7 0.10
mm4__chr9 126.03 Mbp_chr9_126028191_R GTCTCGTGGGCTCGGGCTGAGCTCAGTGTAGGGTG 60.1 0.10
mm4__chr2 19.80 Mbp_chr2_19799443_F TCGTCGGCAGCGTCGCCGAAGAGTCCAAAACAGC 59.7 0.09
mm4__chr2 19.80 Mbp_chr2_19799443_R GTCTCGTGGGCTCGGACTCCTCTCCCTTGTCCCTC 59.9 0.09
mm4__chr2 219.49 Mbp_chr2_219493472_F TCGTCGGCAGCGTCTGTCCTCCTGTCCAGTGGAT 59.8 0.09
mm4__chr2 219.49 Mbp_chr2_219493472_R GTCTCGTGGGCTCGGCGGATGGAAGGGGAAACTTCA 59.9 0.09
mm4__chr10 71.97 Mbp_chr10_71970608_F Primer3: not found at this Tm N.d. 0.09
mm4__chr10 71.97 Mbp_chr10_71970608_R Primer3: not found at this Tm N.d. 0.09
mm2__chr14 103.22 Mbp_chr14_103220211_F TCGTCGGCAGCGTCAACCTGAAGCCCTGGAGATG 59.3 0.08
mm2__chr14 103.22 Mbp_chr14_103220211_R GTCTCGTGGGCTCGGGGTGGGGACGAAGAAGTGAG 60.0 0.08
mm3__chr15 34.79 Mbp_chr15_34794701_F TCGTCGGCAGCGTCGGGAAGTTTACCTGGTGCCG 60.9 0.08
mm3__chr15 34.79 Mbp_chr15_34794701_R GTCTCGTGGGCTCGGATGTGTGACGACGAGGAGAC 59.4 0.08
mm4__chr11 4.65 Mbp_chr11_4647464_F TCGTCGGCAGCGTCCTCTGCCTGTCTTGTGGAGG 60.0 0.08
mm4__chr11 4.65 Mbp_chr11_4647464_R GTCTCGTGGGCTCGGAACCAGACAGCAGGAGAGAA 58.2 0.08
mm4__chr1 3.52 Mbp_chr1_3516650_F TCGTCGGCAGCGTCCATAGCGGATGGGAAGCCC 60.3 0.08
mm4__chr1 3.52 Mbp_chr1_3516650_R GTCTCGTGGGCTCGGTAAGCCAGCTTGCTCTGAGG 59.7 0.08
mm4__chr22 43.33 Mbp_chr22_43328753_F TCGTCGGCAGCGTCTGGCCTAGTGCAATTCGGTT 59.9 0.08
mm4__chr22 43.33 Mbp_chr22_43328753_R GTCTCGTGGGCTCGGTGGAGTTGCCACTGCTGTTA 59.5 0.08
mm4__chrX 46.29 Mbp_chrX_46289028_F Primer3: not found at this Tm N.d. 0.07
mm4__chrX 46.29 Mbp_chrX_46289028_R Primer3: not found at this Tm N.d. 0.07
mm4__chr10 79.40 Mbp_chr10_79399157_F TCGTCGGCAGCGTCTCGGTAGAGGTGGCCTACAG 60.3 0.07
mm4__chr10 79.40 Mbp_chr10_79399157_R GTCTCGTGGGCTCGGGCTCCTTGCTGCTAAGAGCT 60.1 0.07
mm4__chr18 37.41 Mbp_chr18_37410025_F TCGTCGGCAGCGTCCCCAGCCTGAAAGCTCTTCT 59.6 0.06
mm4__chr18 37.41 Mbp_chr18_37410025_R GTCTCGTGGGCTCGGGCTCAGTCCAGTCAACACCT 59.6 0.06
mm4__chr19 33.21 Mbp_chr19_33211507_F TCGTCGGCAGCGTCATGGCCGTGAAGTAGGCAAT 59.7 0.06
mm4__chr19 33.21 Mbp_chr19_33211507_R GTCTCGTGGGCTCGGCTCCACTGCCTGTCACCAG 60.0 0.06
mm4__chr9 134.05 Mbp_chr9_134051380_F TCGTCGGCAGCGTCTAGACCATGAGCTCCTCCCC 60.1 0.05
mm4__chr9 134.05 Mbp_chr9_134051380_R GTCTCGTGGGCTCGGCTTGGATGAGTGTGGCCTGT 59.9 0.05
mm4__chr11 68.43 Mbp_chr11_68427288_F Primer3: not found at this Tm N.d. 0.04
mm4__chr11 68.43 Mbp_chr11_68427288_R Primer3: not found at this Tm N.d. 0.04
mm4__chr1 117.84 Mbp_chr1_117843031_F Primer3: not found at this Tm N.d. 0.04
mm4__chr1 117.84 Mbp_chr1_117843031_R Primer3: not found at this Tm N.d. 0.04
mm4__chr2 218.99 Mbp_chr2_218985181_F Primer3: not found at this Tm N.d. 0.04
mm4__chr2 218.99 Mbp_chr2_218985181_R Primer3: not found at this Tm N.d. 0.04
mm4__chr17 67.43 Mbp_chr17_67433410_F TCGTCGGCAGCGTCCTGTGCTTCTGGGCAGATGA 60.0 0.04
mm4__chr17 67.43 Mbp_chr17_67433410_R GTCTCGTGGGCTCGGTCCCCAGCTTCCTCTCTGTT 60.1 0.04
mm4__chr18 43.09 Mbp_chr18_43090838_F Primer3: not found at this Tm N.d. 0.04
mm4__chr18 43.09 Mbp_chr18_43090838_R Primer3: not found at this Tm N.d. 0.04
mm4__chr14 99.25 Mbp_chr14_99254716_F TCGTCGGCAGCGTCTCATGGAGGGATTGGAGGACT 59.9 0.03
mm4__chr14 99.25 Mbp_chr14_99254716_R GTCTCGTGGGCTCGGAGGAATCACGGGCACTTCTC 59.7 0.03
mm4__chr11 62.77 Mbp_chr11_62766153_F TCGTCGGCAGCGTCTTCTGTGCTCTTCTGCCAGG 59.9 0.03
mm4__chr11 62.77 Mbp_chr11_62766153_R GTCTCGTGGGCTCGGGAGCACAGAACCAACCTCCA 59.8 0.03
mm4__chr6 13.38 Mbp_chr6_13376137_F Primer3: not found at this Tm N.d. 0.03
mm4__chr6 13.38 Mbp_chr6_13376137_R Primer3: not found at this Tm N.d. 0.03
mm4__chr16 88.65 Mbp_chr16_88653520_F Primer3: not found at this Tm N.d. 0.03
mm4__chr16 88.65 Mbp_chr16_88653520_R Primer3: not found at this Tm N.d. 0.03
mm4__chr9 129.40 Mbp_chr9_129402612_F Primer3: not found at this Tm N.d. 0.03
mm4__chr9 129.40 Mbp_chr9_129402612_R Primer3: not found at this Tm N.d. 0.03
mm4__chr3 169.83 Mbp_chr3_169827123_F TCGTCGGCAGCGTCTCAGCTGAGCTCTAGCCAGA 60.0 0.03
mm4__chr3 169.83 Mbp_chr3_169827123_R GTCTCGTGGGCTCGGATCCTTCCCCACAGTTCCCT 60.1 0.03
mm4__chr19 15.45 Mbp_chr19_15445453_F TCGTCGGCAGCGTCCTCCATCCAGAGAAGTGGCG 60.1 0.02
mm4__chr19 15.45 Mbp_chr19_15445453_R GTCTCGTGGGCTCGGCCCCTTGAGACATCGCTGAA 59.7 0.02
mm4__chr3 42.96 Mbp_chr3_42957197_F Primer3: not found at this Tm N.d. 0.02
mm4__chr3 42.96 Mbp_chr3_42957197_R Primer3: not found at this Tm N.d. 0.02
mm4__chr17 65.86 Mbp_chr17_65864212_F TCGTCGGCAGCGTCGCCTCCTGGGAACTCTGGAT 60.9 0.02
mm4__chr17 65.86 Mbp_chr17_65864212_R GTCTCGTGGGCTCGGAACATAGACAACCCAGCATCTT 57.6 0.02
mm4__chr12 10.55 Mbp_chr12_10551598_F Primer3: not found at this Tm N.d. 0.02
mm4__chr12 10.55 Mbp_chr12_10551598_R Primer3: not found at this Tm N.d. 0.02
mm4__chr13 20.90 Mbp_chr13_20902479_F TCGTCGGCAGCGTCCCACTATATTCCCACGCCCG 60.3 0.02
mm4__chr13 20.90 Mbp_chr13_20902479_R GTCTCGTGGGCTCGGCTGGAAGTGGAGGAGGAGGT 60.2 0.02
mm4__chr1 2.12 Mbp_chr1_2116513_F Primer3: not found at this Tm N.d. 0.02
mm4__chr1 2.12 Mbp_chr1_2116513_R Primer3: not found at this Tm N.d. 0.02
mm4__chr17 44.97 Mbp_chr17_44968834_F TCGTCGGCAGCGTCCAGGTCTGCAGGGGACAAAG 60.6 0.02
mm4__chr17 44.97 Mbp_chr17_44968834_R GTCTCGTGGGCTCGGGGTCCCCTCCGTATCCTCTT 60.1 0.02
mm2__chr3 175.98 Mbp_chr3_175978478_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 0.02
mm2__chr3 175.98 Mbp_chr3_175978478_R GTCTCGTGGGCTCGGGCAAAGGCTCCTGTATGTGC 59.5 0.02
mm3__chr3 123.88 Mbp_chr3_123883779_F TCGTCGGCAGCGTCGAACAAGTCTGCAAAGCCCG 60.0 0.01
mm3__chr3 123.88 Mbp_chr3_123883779_R GTCTCGTGGGCTCGGAGGTCCTTCCAGCCCAAATG 59.9 0.01
mm4__chr4 169.38 Mbp_chr4_169383157_F Primer3: not found at this Tm N.d. 0.01
mm4__chr4 169.38 Mbp_chr4_169383157_R Primer3: not found at this Tm N.d. 0.01
mm4__chr18 21.68 Mbp_chr18_21681523_F Primer3: not found at this Tm N.d. 0.01
mm4__chr18 21.68 Mbp_chr18_21681523_R Primer3: not found at this Tm N.d. 0.01
mm4__chr12 56.21 Mbp_chr12_56207419_F Primer3: not found at this Tm N.d. 0.01
mm4__chr12 56.21 Mbp_chr12_56207419_R Primer3: not found at this Tm N.d. 0.01
mm4__chr22 17.11 Mbp_chr22_17110849_F Primer3: not found at this Tm N.d. 0.01
mm4__chr22 17.11 Mbp_chr22_17110849_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 9.38 Mbp_chr1_9383325_F TCGTCGGCAGCGTCGACCTTGTGCAGAGCAGATG 58.9 0.01
mm4__chr1 9.38 Mbp_chr1_9383325_R GTCTCGTGGGCTCGGTTCCTTGCTGTCAGCCTTCC 60.2 0.01
mm4__chr7 68.85 Mbp_chr7_68845018_F Primer3: not found at this Tm N.d. 0.01
mm4__chr7 68.85 Mbp_chr7_68845018_R Primer3: not found at this Tm N.d. 0.01
mm4__chr2 176.20 Mbp_chr2_176204411_F Primer3: not found at this Tm N.d. 0.01
mm4__chr2 176.20 Mbp_chr2_176204411_R Primer3: not found at this Tm N.d. 0.01
mm4__chr6 17.39 Mbp_chr6_17393456_F Primer3: not found at this Tm N.d. 0.01
mm4__chr6 17.39 Mbp_chr6_17393456_R Primer3: not found at this Tm N.d. 0.01
mm4__chr4 9.78 Mbp_chr4_9783013_F TCGTCGGCAGCGTCGTCCCTTTCTGCAGTGGACA 59.8 0.01
mm4__chr4 9.78 Mbp_chr4_9783013_R GTCTCGTGGGCTCGGAAGTCGGCGTTGAAGGCATA 60.0 0.01
mm4__chr2 91.68 Mbp_chr2_91684868_F TCGTCGGCAGCGTCGTTGAGTGAGGAGTTGGCCC 60.6 0.01
mm4__chr2 91.68 Mbp_chr2_91684868_R GTCTCGTGGGCTCGGGGCTGCCCTTCTTCATCCTT 60.0 0.01
mm4__chr2 89.61 Mbp_chr2_89610607_F TCGTCGGCAGCGTCGTTGAGTGAGGAGTTGGCCC 60.6 0.01
mm4__chr2 89.61 Mbp_chr2_89610607_R GTCTCGTGGGCTCGGGGCTGCCCTTCTTCATCCTT 60.0 0.01
mm4__chr1 144.69 Mbp_chr1_144691102_F TCGTCGGCAGCGTCGTCCCTTTCTGCAGTGGACA 59.8 0.01
mm4__chr1 144.69 Mbp_chr1_144691102_R GTCTCGTGGGCTCGGAAGTCGGCGTTGAAGGCATA 60.0 0.01
mm4__chr1 144.28 Mbp_chr1_144275250_F TCGTCGGCAGCGTCAAGTCGGCGTTGAAGGCATA 60.0 0.01
mm4__chr1 144.28 Mbp_chr1_144275250_R GTCTCGTGGGCTCGGTCCCTTTCTGCAGTGGACAC 59.8 0.01
mm4__chr1 143.45 Mbp_chr1_143449634_F TCGTCGGCAGCGTCAAGTCGGCGTTGAAGGCATA 60.0 0.01
mm4__chr1 143.45 Mbp_chr1_143449634_R GTCTCGTGGGCTCGGTCCCTTTCTGCAGTGGACAC 59.8 0.01
mm4__chr7 1.30 Mbp_chr7_1302238_F TCGTCGGCAGCGTCTGAAGCCTGGGAGAAACCAC 59.8 0.01
mm4__chr7 1.30 Mbp_chr7_1302238_R GTCTCGTGGGCTCGGGAGGATAACGGCCTGGACAG 59.8 0.01
mm4__chr2 74.83 Mbp_chr2_74834098_F TCGTCGGCAGCGTCGCACGTCACTGATCCGGAG 60.5 0.01
mm4__chr2 74.83 Mbp_chr2_74834098_R GTCTCGTGGGCTCGGGCCGGAGTTTCATGCAACAA 59.6 0.01
mm4__chr2 17.88 Mbp_chr2_17879298_F TCGTCGGCAGCGTCCGTTCCTTCCCTTCCTCGAG 59.8 0.01
mm4__chr2 17.88 Mbp_chr2_17879298_R GTCTCGTGGGCTCGGAAAGGGGAGGGGAGGAATGA 59.8 0.01
mm4__chr5 122.52 Mbp_chr5_122518090_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 122.52 Mbp_chr5_122518090_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 12.99 Mbp_chr19_12985893_F TCGTCGGCAGCGTCGAGATCTGATGCCAGCTGGG 60.2 0.00
mm4__chr19 12.99 Mbp_chr19_12985893_R GTCTCGTGGGCTCGGGCTGCATCTAGAGGCCACTC 60.2 0.00
mm4__chr20 3.65 Mbp_chr20_3646519_F Primer3: not found at this Tm N.d. 0.00
mm4__chr20 3.65 Mbp_chr20_3646519_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 42.02 Mbp_chr19_42022357_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 42.02 Mbp_chr19_42022357_R Primer3: not found at this Tm N.d. 0.00
mm4__chrX 120.47 Mbp_chrX_120468904_F TCGTCGGCAGCGTCTACCGGCCCCTTCTTACTCT 59.6 0.00
mm4__chrX 120.47 Mbp_chrX_120468904_R GTCTCGTGGGCTCGGAGGCTCAGCAGCTAACACTG 60.0 0.00
mm4__chr1 10.83 Mbp_chr1_10834501_F TCGTCGGCAGCGTCGCCAGTGAATCCAGGGAGAC 60.1 0.00
mm4__chr1 10.83 Mbp_chr1_10834501_R GTCTCGTGGGCTCGGTATACCATGCAGTGCCACCC 59.8 0.00
mm4__chr7 75.05 Mbp_chr7_75052694_F TCGTCGGCAGCGTCCTGCAGTGACGTCAGGTGAC 60.9 0.00
mm4__chr7 75.05 Mbp_chr7_75052694_R GTCTCGTGGGCTCGGATGGGGCAAGAACATCCGAG 60.1 0.00
mm4__chr12 115.24 Mbp_chr12_115243991_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 115.24 Mbp_chr12_115243991_R Primer3: not found at this Tm N.d. 0.00
mm4__chrX 108.30 Mbp_chrX_108296282_F TCGTCGGCAGCGTCGTTCACTCTGTCAAACCCAACA 58.9 0.00
mm4__chrX 108.30 Mbp_chrX_108296282_R GTCTCGTGGGCTCGGGGACCACGAAAGGGAAGGAG 60.0 0.00
mm4__chr8 9.33 Mbp_chr8_9329565_F TCGTCGGCAGCGTCTGTTCAGTCAGCACGCTAGG 60.0 0.00
mm4__chr8 9.33 Mbp_chr8_9329565_R GTCTCGTGGGCTCGGACAGCAGTTTCCACGAGTTGA 60.1 0.00
mm4__chr7 78.55 Mbp_chr7_78553868_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 78.55 Mbp_chr7_78553868_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 21.90 Mbp_chr7_21900113_F TCGTCGGCAGCGTCTGCATACCTCCACGGACTCT 60.3 0.00
mm4__chr7 21.90 Mbp_chr7_21900113_R GTCTCGTGGGCTCGGTGTGTGAGTGAGGAGATGCTG 59.7 0.00
mm4__chr7 101.05 Mbp_chr7_101050454_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 101.05 Mbp_chr7_101050454_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 173.64 Mbp_chr5_173644694_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 173.64 Mbp_chr5_173644694_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 135.84 Mbp_chr3_135839633_F TCGTCGGCAGCGTCCCCAGCCTCCAAACCAATGA 60.2 0.00
mm4__chr3 135.84 Mbp_chr3_135839633_R GTCTCGTGGGCTCGGAGGGGTAGAGCAGCATCTCA 60.0 0.00
mm4__chr3 120.45 Mbp_chr3_120451381_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 120.45 Mbp_chr3_120451381_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 25.38 Mbp_chr22_25383035_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 25.38 Mbp_chr22_25383035_R Primer3: not found at this Tm N.d. 0.00
mm4__chr20 62.59 Mbp_chr20_62590947_F TCGTCGGCAGCGTCATTCTGGTTCCCTGAGCAGC 60.0 0.00
mm4__chr20 62.59 Mbp_chr20_62590947_R GTCTCGTGGGCTCGGGCAATGCACTTGGGGACATG 60.1 0.00
mm4__chr2 241.48 Mbp_chr2_241482278_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 241.48 Mbp_chr2_241482278_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 239.28 Mbp_chr2_239275807_F TCGTCGGCAGCGTCGGCAAGATAGAACGCCAGGA 59.8 0.00
mm4__chr2 239.28 Mbp_chr2_239275807_R GTCTCGTGGGCTCGGCATCTTCTCCGCCCCATACC 59.9 0.00
mm4__chr2 216.93 Mbp_chr2_216931440_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 216.93 Mbp_chr2_216931440_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 40.27 Mbp_chr19_40266161_F TCGTCGGCAGCGTCCCACTGCTTCGTCTCAGGTT 59.9 0.00
mm4__chr19 40.27 Mbp_chr19_40266161_R GTCTCGTGGGCTCGGAAAGGCGCCTTGGTCTCTG 60.3 0.00
mm4__chr18 76.67 Mbp_chr18_76674719_F TCGTCGGCAGCGTCCAGGTCTGAATCCCTGCTGG 60.1 0.00
mm4__chr18 76.67 Mbp_chr18_76674719_R GTCTCGTGGGCTCGGCGACCTTGAGAGCCTGTGG 60.0 0.00
mm4__chr17 52.96 Mbp_chr17_52961558_F Primer3: not found at this Tm N.d. 0.00
mm4__chr17 52.96 Mbp_chr17_52961558_R Primer3: not found at this Tm N.d. 0.00
mm4__chr17 44.91 Mbp_chr17_44910383_F TCGTCGGCAGCGTCACAGGGCAGATGTCAAGCTC 60.0 0.00
mm4__chr17 44.91 Mbp_chr17_44910383_R GTCTCGTGGGCTCGGGGGTACTTCTAGGTGGGGCT 60.3 0.00
mm4__chr16 88.99 Mbp_chr16_88993393_F TCGTCGGCAGCGTCCCTCACAGCCTCAGCACTTG 60.9 0.00
mm4__chr16 88.99 Mbp_chr16_88993393_R GTCTCGTGGGCTCGGGGAACCCTCATGTGAACCCC 60.3 0.00
mm4__chr16 50.15 Mbp_chr16_50153388_F Primer3: not found at this Tm N.d. 0.00
mm4__chr16 50.15 Mbp_chr16_50153388_R Primer3: not found at this Tm N.d. 0.00
mm4__chr16 2.97 Mbp_chr16_2973004_F TCGTCGGCAGCGTCTCAGGTTGGGTCTAGGGTGT 59.8 0.00
mm4__chr16 2.97 Mbp_chr16_2973004_R GTCTCGTGGGCTCGGGGATGCCCTGGACCTAAGTG 59.8 0.00
mm4__chr15 78.62 Mbp_chr15_78616685_F Primer3: not found at this Tm N.d. 0.00
mm4__chr15 78.62 Mbp_chr15_78616685_R Primer3: not found at this Tm N.d. 0.00
mm4__chr15 69.39 Mbp_chr15_69393672_F TCGTCGGCAGCGTCGGAGAGAACCACCTTCCCCT 60.5 0.00
mm4__chr15 69.39 Mbp_chr15_69393672_R GTCTCGTGGGCTCGGTCAACCCTGATTTTCGGGCA 59.8 0.00
mm4__chr13 72.77 Mbp_chr13_72772577_F Primer3: not found at this Tm N.d. 0.00
mm4__chr13 72.77 Mbp_chr13_72772577_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 52.18 Mbp_chr12_52180838_F TCGTCGGCAGCGTCTGAGGGCTTTGATTGGGCAT 59.9 0.00
mm4__chr12 52.18 Mbp_chr12_52180838_R GTCTCGTGGGCTCGGGAAGAAGGTAGCCTGCCCAG 60.1 0.00
mm4__chr12 52.00 Mbp_chr12_52003254_F TCGTCGGCAGCGTCGCCCCTCATTCCTGACAGAC 60.1 0.00
mm4__chr12 52.00 Mbp_chr12_52003254_R GTCTCGTGGGCTCGGTGGGTACTGTCACTGCTCCT 60.1 0.00
mm4__chr12 132.32 Mbp_chr12_132321981_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 132.32 Mbp_chr12_132321981_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 92.11 Mbp_chr11_92105960_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 92.11 Mbp_chr11_92105960_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 40.29 Mbp_chr11_40293455_F TCGTCGGCAGCGTCCAGATCCTCCAGGTGTTGCA 59.6 0.00
mm4__chr11 40.29 Mbp_chr11_40293455_R GTCTCGTGGGCTCGGCTCCCGGAGCCACTACCG 61.5 0.00
mm4__chr11 113.35 Mbp_chr11_113348051_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 113.35 Mbp_chr11_113348051_R Primer3: not found at this Tm N.d. 0.00
mm4__chr10 117.60 Mbp_chr10_117602904_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 117.60 Mbp_chr10_117602904_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 34.80 Mbp_chr1_34795022_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 34.80 Mbp_chr1_34795022_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 230.87 Mbp_chr1_230868140_F TCGTCGGCAGCGTCCTTTTCCAGCCCCTACCAGC 60.6 0.00
mm4__chr1 230.87 Mbp_chr1_230868140_R GTCTCGTGGGCTCGGCCAGAAGGTGGTGCGCTT 60.2 0.00
mm3__chr5 138.25 Mbp_chr5_138252935_F TCGTCGGCAGCGTCCTGCTGCTGTCCTTTCCTCA 59.9 0.00
mm3__chr5 138.25 Mbp_chr5_138252935_R GTCTCGTGGGCTCGGTGCACGCTTCCCTTGATTCT 59.9 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529556 CTCCCCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCT
TGCACATGCCGGAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATCCA
mm0__chr5 131.66 Mbp_chr5_131660015 ATGCCCATCATCACACCCTGGTGCCTCGGGTGCCCCATTATGGAGGGGAAGACGGCCCGGGGGCATCGTTGCCCGTGAAG
CAGACCTTCCACA
mm2__chr22 15.72 Mbp_chr22_15721564 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm2__chr2 131.26 Mbp_chr2_131263633 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGCGCCCCAGGCAGCAGGGCATGATGGGGGGCATGCATCAGAAAGAG
mm2__chr2 130.66 Mbp_chr2_130656827 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCAGCAGGGCATGATGGGGGGCATGCATCAGAAAGAG
mm2__chr2 130.46 Mbp_chr2_130463842 CTCTTTCTGATGCATGCCCCCCATCATGCCCTGCTGCCTGGGGCGCCCCACGATGGAAGGGAAGACAGCCCGGGGGGCAT
CGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm2__chr2 130.08 Mbp_chr2_130075270 CTCTTTCTGATGCATGCCCCCCATCATGCCCTGCTGCCTGGGGCGCCCCACGATGGAAGGGAAGACAGCCCGGGGGGCAT
CGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm2__chr14 19.40 Mbp_chr14_19402807 ATCATGTTCTGGTGCCTGGGGCACCCCACGATGGAAGGGAAGACAGCCCGGGGGGCATCGTCACCTGCAAAGCCGGCCTT
GCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm2__chr14 19.00 Mbp_chr14_18998945 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm1__chr2 73.90 Mbp_chr2_73901392 AGGCTTCGCAGGAGATGATGCCCCCCGGGCTGTCTTCCCCTCCATTGTGGGCCGCCCTCGCCACCAGGTGCGTGCTCATC
TGGATA
mm1__chr11 1.80 Mbp_chr11_1803994 CGCCACCCACATAGAAGTCCTTCTGACCCATGCCCACCGTCATGCCCTGGTGTCTGGGGCACCCCACGATGGAGGGGAAG
ACGGCCCAGGGGGCATCGTCGTCGGTGAAGCTGGCCTTGCACATGCCAGAGGTGTT
mm1__chr19 9.52 Mbp_chr19_9518227 AGTTAACAATGGCTCCGGCATGTGCAAGGTCAACTTCGTGGGCGACAATGCCCCCCGGGCCATCTTCCCCTCCATCGTGG
GGCATCTGAAGCACCAGGGCGTTATGGTGGGCATCGGTCAGAAGGACTCC
mm3__chr22 41.08 Mbp_chr22_41075138 GGAGTCCTTCTGACCCATGCCCACCATCATGACCTGGTGCCTGGTGCACCCCACGATGGAAGGGAAGATGGCCCAGGGGA
CATCGTCACCCGCGAAGCCGGCCTTGCACATGCCAGAGCTGTTGTCAACA
mm3__chr1 223.86 Mbp_chr1_223864750 TGACCCATGCTCACCATCACGCCCTGGTGCCTGGGGCGCCCCACCATGGAAGGGAAGATGGCCCAGGGGTCATCATCCCC
TGTGAAGCCAGCCTTGCACATGCCGGAGCTGTTGTCAATGACGAGCG
mm2__chr15 43.99 Mbp_chr15_43990081 GAGTCCTTCTGACCCATGCCCACCGTCACGCCCTGGTGTCTGGGGCGCCCCACAATGAAGGGGAAGATGGCCCGGGGTGG
GCATCGTCACCTGCAAAGCTGGCCTTGCACATGCCAGAGCCATTGTCGACGACA
mm3__chr5 80.30 Mbp_chr5_80302746 CTCTTGCTCTGGGCCTCATCCACATAGGAGTCCTTCTGACCCATGCCCACCATCATGCCCTGGTGCCATGATGAAGGGGA
AGGCAGCCCGGGGGACACCATCGCTCGCAAAGCTAGCCTTGCATATGCCA
mm2__chr5 57.48 Mbp_chr5_57482603 ATCTCCCACGTAGCAGTCCTTCTGGCCCATGCCTACCATAACGCCCTGGTGTCGAGGACGCCCTATCATGGAGGGGAACA
CAGCCCGGGGGGCATCGTCACCACCAAAGCCTGCCTTGCACATCCCTGACCCAT
mm3__chr5 98.34 Mbp_chr5_98338820 GCTCCTCATCAACAAGGGCTCTGGCATGTGCAAGGCCGGCTTCACAGGTGACAATGCCCCCGGGCCATCTTCCCCTCTGT
CATGGGTGCCAGGGAGTGATGGTGGGCATGGGTCAAAA
mm1__chr6 88.28 Mbp_chr6_88276906 GAGTCCTTCTGACCCATGCCCACCATCACTCCCTGGTGCCTAGGGTGCCCCACAATGGAGGGGAAGACGGCCTGGGGAGC
CTTGCGCATGCTGGAGCAGTTGTCGACGACGACGAGCGCGGTGATAGCATCATCCA
mm4__chr10 88.95 Mbp_chr10_88948823 CTCACCTGATGTCTGGGACGTCCCACAATGGATGGGAAAACAGCCCTGGGAGCATCGTCCCCAGCAAAGCCGGCCTTACA
GAGCCCAGAGCCATTGTCACACACCAAGGCAGT
mm4__chr2 131.63 Mbp_chr2_131626216 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCAGGCTGTCTTCCCTTC
TATCGTGGGGCGCCCCAGGCACCAGGGCATGATGGAGGGCATGCATCAGAAGGAGT
mm3__chr3 12.07 Mbp_chr3_12070302 TCATTGACAGTGTCTCCGGCATGTACAAAGCTGGATTTGCTGGGGACGACGCCCCCTGAGCCGTGTTCCCCTCCATCCTT
AGGCGCCCCTGGCACCAGGGCACGATGGTGGGCATGGGTCAGAAGGACTC
mm3__chr17 44.26 Mbp_chr17_44262508 CACTGGATGGGTGACTGCCCCTGCTCTCCCAGCCACCTCCTGTGATCATTTCAAACAAAGCTCTGTTTATCCTTCAATCA
GCCAGAATTGAGGGGAGAACGGCCCGTGGTGCTTGGGAGGAGGACTGTGGAGAA
mm4__chrX 53.14 Mbp_chrX_53143811 CATGGCCCATGTAGGAGTCCTTCTGGCCCATGCCCACCATCACACCCTGGTGCCGGGGTCACCTGATGATGGAGGGGAAC
ACAGCTCAGGGGCCATCCATCCCCAGCAAAGCCAGCTTTGCACATGCCAGAG
mm3__chr2 239.39 Mbp_chr2_239392592 CAAGCAAGGAGGCACAGTTGGCATCACTAATTAAGATACACCCCGCTTGTGTGGACCCACAGGCCATCTTCCCCTCCTTG
TCCACAGCCCCCAGAGGTGGAATGGGGGTCAGCCTCCTTAGAAC
mm4__chr2 43.16 Mbp_chr2_43158724 AGGCCAGGGAGGAGAGTTAGACTGAGCAAAAGCAGGATGTGTGTGGAGCAGCCTCCTGGCCTCTCTCCCGCAGAGGCACT
TCCTGGTACTGGAGGGCAAAGCGGCCCGGGGTTGGGAAAGAGACCGGTGCTGCTTG
mm4__chr7 98.82 Mbp_chr7_98822872 GCCAAGGCCCCTTAGATCTTGTCCTAAAGCTGGGGATGAGTCACCTGTGAATCAGGCAGCAGGCTGAGCCAGCACTGCTC
TCCAGGGGCCGCCTTCCCTTCCAAAGGACTAGCCTGTGTAAGGGGC
mm3__chr20 63.31 Mbp_chr20_63305080 ATAGGGTAGGAGCCTGTGGGAGTGGGCAGGGCCCTGGCCCCGGACTCTTCCTTCTTGGACCTGGCCCCTCGGGTCGTCAT
CCCCTCCCTGGGCAGCCCTGTCTTCATAC
mm3__chr1 229.43 Mbp_chr1_229433008 GCAGCCTGACCTGGTGTCGGGGGCGGCCCACGATGGACGGGAACACGGCCCTAGGGGCGTCATCCCCGGCGAAGCCGGCT
TTCACCAGGCCGGAGCCATTGTCGCACACGAGGGCGGTGGTCTCGTCTTCGTC
mm4__chr7 100.89 Mbp_chr7_100890359 CACCATGTAGGAGCTCCAGCGGTGGAACTCGGCCTTCCACTGGCGCTCCGCCTCGTCGAGCGTGTCTGCGGCCAGGGCGC
CCAGCGAGGCGGGAGGGGAGGACGGCACGAGGAAGGTCGGGGATCGGACATTTG
mm3__chr1 202.87 Mbp_chr1_202873369 TGACAATGGCTCCAGCACATGCAAAGCTGGCTTTGCTGGGTATAGTTCCCCGAGCCATGTTCCCCTCCATCACTGGGTAT
CCCTGGCACCAGGGCATGATGGTGGGCATAGGCCAGAAGGACTCC
mm4__chr17 81.51 Mbp_chr17_81512253 CCATCCACTCACCTGGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCGTCCCCAGCAAAACCAG
CTTTGCACATGCCGGAGCCATTGTCAATGACCAGCGCGGCGATCTCTTCTTCCAT
mm4__chr20 51.59 Mbp_chr20_51586257 GTGGCAATTCCCTGGGAAGTGCTATGTCAGGGATGGAGGTGAGGATGGCCCTTGGGAATGATGGGATTCAGATAAATGGA
GCATTAATTTGGAAATCTGCCTTGGCATTTTTCAACCTCCTGTGACATGCC
mm4__chr8 100.73 Mbp_chr8_100725167 CCTGATGGTCACATGGGCACCTAGGGCCGTGTTCACCTCCAGACTGCTTGGCCTGCTGCAGCATGTTACCTCTCAGCTCC
ACAAGCTGTTTCGGGGAGCGCTTCCTGACATGCTGGTTGGGCTCTCATTT
mm4__chr11 789.27 Kbp_chr11_789273 CTCTCCAGGAAGTCAGCGTGGGGCTGTGCTTCCAAGGGCCGTCTGCCCCTCCCCAGCCTCTGGGGCCCCCTCCTCAGCAT
GGGCCGTGCCTGCCTCCAGCCCTGGAGCAGGGAGTCCTCG
mm4__chr6 30.26 Mbp_chr6_30260823 TAACTCTGAGAGCCCACCCTGCTCTCTGGGACAATTAAGGGATGAAGTCCCTGAGGAAATGGAGGAGAAGACAGTCCCTG
GAATACTGATCCGTGGTCCCCTTTGACCCCTGCAGCAGCCTGGGGCACCAGGAATTTTCC
mm3__chr6 31.27 Mbp_chr6_31270853 CTGCTGCAGTGGTCAAAGTGGTCAAAGGAGACCCCTGATCAGTATTCCAGGGACTGTCTTCCCCTCCATTTCCTCAGAGA
CTTCATCCCTTAATTGTCCTAGAGAGCAGAGGGGGCCCTCAGAGGAAACTCAG
mm4__chr21 43.27 Mbp_chr21_43267526 CTCCAAGAAGCCCTGCAGTCCTGCAAACGAGAAGGAAACAGTACTTCTTCCCTTCCCTGAGCTTCCACGGGCTTTGTTCC
CCTCCACATGGGAGGCGCCTCTGCTGAAAAGGCCGGCCTGATA
mm4__chr5 17.23 Mbp_chr5_17225397 CACTACCTGCTGCCACTTCATCCACAGGCCATCTTCCCCTCAACTTACTCCTTTCTGCTTCTTACATTATGTGCACGTGG
TTTCCTTT
mm4__chr19 48.20 Mbp_chr19_48196453 GTATTGGAAGGCAGCCCAGAATGGGCAGCGGCGAGGAGTGAACACCTGGCTGCAGGTGACGGCCTGCAGGAAGGAGGCGA
AGATGGCCCCAGGGAACCAAAGAGGCTTTGCCGACCCCCGGGAGAGGAGGAGGTGGACTG
mm4__chr8 84.95 Mbp_chr8_84948666 CTCTGGTATGTGCAAGGCCAGAATCCTTGTCAAAGATGCCCCCAGGCCTTCTTCCTTTCCATAGTGGGATGCCCATGGGA
CCAGAGTTTGATGATGGGCATAGGGCAGAAGGACTG
mm4__chr1 81.80 Mbp_chr1_81801131 TATCCCCTTTCGCTCCTCCTCCCCGCCGCTTGGGCGCACAGCCGCAGCAGCTGCTCAAATGGAGTGGAAAACGGCCATTG
GGCGACAATGAGTTATAGATTATCTGCTCCACGTTTTGTACTTAGCTGCC
mm4__chr15 24.95 Mbp_chr15_24947265 AGAGACCCTGCCACAAAAGTACTCCTCATGGAAGGGAAGATGGACTGGGGATGATGTGGCAGTTATACAGGGA
mm4__chr14 95.85 Mbp_chr14_95850047 GCTAGAACTCTAGTCCCTCCCAATGTATTTCTTCTGCCACTGGCCATCTTCCACTCCCTTCCTTGTCCATTTTTCCATGC
AGGCCAAGCTCAAATGCTG
mm4__chr3 45.13 Mbp_chr3_45128222 GACCTGGGGAGTGATCAAGCACAACCTCCCACCTGCAGTGGGCCTGAGACACAGGACTGCACTGGGAAGTCAGGGCAGGG
AAGAAGGCCCGAGGGAGTAGGACAAGGGCATGCAC
mm4__chr7 43.13 Mbp_chr7_43131946 TGGCACAAGTGTTCACCCTTTGTCCTTCTGTCTCCTCCTGCTGTCTTATGACCGAGGATTGAAAGCTGCATGCTGAGGAC
TCCACATGGAGGGAAAGGTGGCCTGGGGGCCTGGGTTACAAGGGCATCGA
mm4__chr20 61.06 Mbp_chr20_61057786 TGAACGACACTCCAGGAAGCTCAGCGGGCTGGGAGCCAGGACCTCGCACACTGGAAGGGGGGTTCCCAGTGCCCAGAGCT
GTCTGCCCCTCCATCAGCCCCTGAGCTGACTGTCTTTGCTTTCCTGGGGAGTCGATC
mm4__chr17 58.50 Mbp_chr17_58504251 CAGAGAGAGCTTGCACCTGGGGGCCTCGGGCTGTCATTCCCCCCATCCCTGTTGTCACAGGCCTTAGGACTTACCAGGAA
TGCTTCATTAAATTCAAACAGGCAGGGGAACTG
mm4__chr7 43.73 Mbp_chr7_43729870 TTGCTCCTCACAGGTATGCGGCGCTTCCGCTGGCCTCCAAGGACACGAAGCCCCGGGCCGTCTGGCCCCCCACTCTAGCA
CTGAAATTCCTGCCGCCCCGTTCAGCTGACAAATTTGGCAGC
mm4__chr17 49.00 Mbp_chr17_48997575 GCACTTCTCCTGGGCTCTCCCCGAACTCTCCCGCGACCTCTGCGCGCCCTCAGGCCGCCTTCCCCGCCCTGGGCTCGGGA
CAACTTCTGGGGTGGGGTGCAAAGAAAGT
mm2__chr18 62.44 Mbp_chr18_62442086 CCTCGTCATCGACAATGGCTCTGGCATGTGCAGGGCTGGCTTCGCAGGCGACAGTGCTCTCCAGGCCGTCTTCCTCTCCA
TCATGGGGCACCCCAGGCACCAGGGTGTGATGGTGGGCATGGGTCAGAAGGACTC
mm4__chr6 101.43 Mbp_chr6_101431436 TGATGCTGTCCTGTGTGCTTACTAGAAAGACAAGTCTTTACTCTGACAGAGTTTATATTCTGGTGCTGGGGGCGCCCAAC
AATGGAGGGGAACATGGCTTGGGGGCGTCATCCCCAGCAAAGCCG
mm2__chr9 6.83 Mbp_chr9_6834532 TGATATTGCTACGCCCGTCGTCGACAACGGCTCCGGCATGTGGAAGGCTGGCTTCGCAGGTGACGATGACCCCCGGCCGT
CTTCCCCTTCATCGTGTGGCACCCCAAGCACCAGGGCATGATGGTGGGCATGGGT
mm4__chr1 6.27 Mbp_chr1_6270858 ATACCTGAGGCCAAGTGCAGCCTGGGAACTGCTCCAAGGACTGTCCTGCTGGTGTGTGGCTGCCCCAAAGGAGGGCCAGA
TGGCCCGGGGTCACCTTAACGCAGCGGGCCCTTTAGGATGTCACA
mm4__chr9 126.03 Mbp_chr9_126028191 ACAGAGCTCTCATCTCCCGATCTCTGCCCCCCAGGCCTTCCTCCCCTCCAAGAGTTGNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNAAGCAGCTGAACATTTAGTGAGCACTCACCCTACACTGAGCTCAGC
mm4__chr2 19.80 Mbp_chr2_19799443 GCCGAAGAGTCCAAAACAGCATAATGGGTGCTATGATGGAAGGGAAGAAGGCTCCGGGGGAGGGACAAGGGAGAGGAGT
mm4__chr2 219.49 Mbp_chr2_219493472 TGTCCTCCTGTCCAGTGGATACAGCCCTGGGCGCTCTGCTGGCCCAAGGATGTCCCCACTGCCCCTCCATGGCCTCTGGC
CTTCTTCCCATTCATATTTATTTATTTATTGACTTTTATGAAGTTTCCCCTTCCATCCG
mm2__chr14 103.22 Mbp_chr14_103220211 AACCTGAAGCCCTGGAGATGGCCTGGCTGGAGGGGACGACGGCCCGCAGCGGGGACCCGACTCACTTCTTCGTCCCCACC
mm3__chr15 34.79 Mbp_chr15_34794701 GGGAAGTTTACCTGGTGCCGCGGGCGGCCCACGATGGACGGGAAGACAGCGCGGGGCGCGTCATCGCCCGCAAAGCCGGC
CTTCACCAGCCCAGAGCCGTTGTCGCACACCAGGGCGGTGGTCTCCTCGTCGTCACACAT
mm4__chr11 4.65 Mbp_chr11_4647464 CTCTGCCTGTCTTGTGGAGGGACTATGGAACATTGGGATGCTTCAGGGCTGGCCAGTCTGAAGGGTGGCACTTGGGCCCT
CTGGCTGGCTTCCTCTCCATCCCTTCATTTCTCTCCTGCTGTCTGGTT
mm4__chr1 3.52 Mbp_chr1_3516650 CATAGCGGATGGGAAGCCCGGCACCATGGAGGGGAGGGCGGTCAGAGGTCAACAGAGCAGGCAGCGGCTGGAGCAGCGGT
GTTGCCTCTGGGCCCTGAGAAGGCAAAGCCCCCTCAGAGCAAGCTGGCTTA
mm4__chr22 43.33 Mbp_chr22_43328753 TGGCCTAGTGCAATTCGGTTTCATTCCCTTTCTCTCCGAATCCCCGGGTGGACTTCCTCTCCATTGTAATAAAAATAACA
GCAGTGGCAACTCCA
mm4__chr10 79.40 Mbp_chr10_79399157 TCGGTAGAGGTGGCCTACAGGGCAGGGGTAAAGGCAGTGGATGGAGGAGAAGAAGGACTGTGGGCAGCTCTTAGCAGCAA
GGAGC
mm4__chr18 37.41 Mbp_chr18_37410025 CCCAGCCTGAAAGCTCTTCTCTCTCAGGAATTTTGGTTGGCTGCTAATATTGGGATGAGAAAGGCCAATGAGAATGATGG
AGGCGGAGACGGCCTCAGGGCCACTTTCAGGTGTTGACTGGACTGAGC
mm4__chr19 33.21 Mbp_chr19_33211507 ATGGCCGTGAAGTAGGCAATGTTGGTGAACGTGTACACGAAGGTCACCAGTGGGATGGAGATGAAGATGGCGCGAGGTAG
GTTCCTGGTGACAGGCAGTGGAG
mm4__chr9 134.05 Mbp_chr9_134051380 TAGACCATGAGCTCCTCCCCAGCGTGGGCTCAAAGGCAGCCAGCTATGGCGTTAATGTCTTCCGTATCCCCGGGCCAGCT
GCCCCTCCAACATCACCCACAGGCCACACTCATCCAAG
mm4__chr17 67.43 Mbp_chr17_67433410 CTGTGCTTCTGGGCAGATGAATGGAGGGCAAGCCAGCCCCGGGCAGAAGATAGAACAGAGAGGAAGCTGGGGA
mm4__chr14 99.25 Mbp_chr14_99254716 TCATGGAGGGATTGGAGGACTCCTTGGAGCCCCGGCCAGGAGGCAGAAGCCTGGAGGGACAGCCGGCCCGGGGGCCAGGA
GCGTGGGCAGCAGCTGGACGGGTTTCCGGAAGCCTGGGCGGAGAAGTGCCCGTGATTCCT
mm4__chr11 62.77 Mbp_chr11_62766153 TTCTGTGCTCTTCTGCCAGGAAGAAGGGATGGATGGGCAGAAGGCCTGGGGCTGCAGGGTGGAGGTTGGTTCTGTGCTC
mm4__chr3 169.83 Mbp_chr3_169827123 TCAGCTGAGCTCTAGCCAGAATGAAAGTAGGGTGGCTGACTAGGGAATGGATGGGAAGAATGCCCATGGGCTCAAGCAGG
GAACTGTGGGGAAGGAT
mm4__chr19 15.45 Mbp_chr19_15445453 CTCCATCCAGAGAAGTGGCGGTGGAGGGGAAGAAGGCTGGTGGGAGGCCTGGGGGATCAGGGCTGGTTCAGCGATGTCTC
AAGGGG
mm4__chr17 65.86 Mbp_chr17_65864212 GCCTCCTGGGAACTCTGGATTTGACACAGGTTTCAGATCTTTCAGATTTTTGATCCTCGGGCAGTTTGCCACTCCATGTG
TCCACCTAACTATCCATTACCTCTTCGAGTCATATTTTAAGATGCTGGGTTGTCTATGTT
mm4__chr13 20.90 Mbp_chr13_20902479 CCACTATATTCCCACGCCCGACTGGGGTGCTGGGCAGCCAGGGGAAGGGGACAGCTCCTGGAAGGAGGGGACGACGGCAG
GAGGAAAGTAGGCTGAAGAATCCTGCGCCACGCCACCGCCACCTCCTCCTCCACTTCCAG
mm4__chr17 44.97 Mbp_chr17_44968834 CAGGTCTGCAGGGGACAAAGAGCGATTGAGGGGAGGACGGAGCGGGGAGCTGGAAGGTAGGATGAGAAAGGGGGTTATGA
AGCGAAGAAGAGGATACGGAGGGGACC
mm2__chr3 175.98 Mbp_chr3_175978478 GAGTCCTTCTGACCCATGCCCACCATCACACCTTGGTGCCTGGGGCGCCCCACGATGGATGGGAAGACGGCCCCGGAGGC
ATCGTCGCCTGCGAAGCTGGTCTTGCACATACAGGAGCCTTTGC
mm3__chr3 123.88 Mbp_chr3_123883779 GAACAAGTCTGCAAAGCCCGCGCCCCAGCTTCGCCTGGGATGGCCAGGTCTGGCACTCGCGGGCCGCCTTCCCCGCCCTA
CCTTCCCGGAATCACATTTGGGCTGGAAGGACCT
mm4__chr1 9.38 Mbp_chr1_9383325 GACCTTGTGCAGAGCAGATGGCGCGGGTGTTCAAGGAGGGGAAGATGGGCCCTGGGGAGGGAGGAAAGCCACCTCCAGGC
TCGGGGCTGCAGACCAGAGGGGTGGGAACTGGCAAGGGGAAGGCTGACAGCAAGGAA
mm4__chr4 9.78 Mbp_chr4_9783013 GTCCCTTTCTGCAGTGGACACCCCGAAGGCCCTCCGGCCGGCTTCCCCTGCGTCAGTGAGACCACCTTCGACGTCTTCGT
CTGGTTCGGCTGGGCTAACTCCTCACTCAACCCCGTCATCTATGCCTTCAACGCCGACTT
mm4__chr2 91.68 Mbp_chr2_91684868 GTTGAGTGAGGAGTTGGCCCAGCAGAACCAGATGAAGACATCGAATGTGGTCTCACTGACGCAGGGGAAGCCGGCCGGAG
GGCCTTTGGGGTGTCCACTGCAGAAAGGAACCATGCAGTTAAGGATGAAGAAGGGCAGCC
mm4__chr2 89.61 Mbp_chr2_89610607 GTTGAGTGAGGAGTTGGCCCAGCAGAACCAGATGAAGACATCGAATGTGGTCTCACTGACGCAGGGGAAGCCGGCCGGAG
GGCCTTTGGGGTGTCCACTGCGGAAAGGGACCATGCAGTTAAGGATGAAGAAGGGCAGCC
mm4__chr1 144.69 Mbp_chr1_144691102 GTCCCTTTCTGCAGTGGACACCCCAAAGGCCCTCCGGCCGGCTTCCCCTGCGTCAGTGAGACCACATTCGACGTCTTCAT
CTGGTTCTGCTGGGCCAACTCCTCACTCAACCCAGTCACTATGCCTTCAACGCCGACTT
mm4__chr1 144.28 Mbp_chr1_144275250 AAGTCGGCGTTGAAGGCATAGTGACTGGGTTGAGTGAGGAGTTGGCCCAGCAGAACCAGATGAAGACGTCGAATGTGGTC
TCACTGACGCAGGGGAAGCCGGCCGGAGGGCCTTTGGGGTGTCCACTGCAGAAAGGGA
mm4__chr1 143.45 Mbp_chr1_143449634 AAGTCGGCGTTGAAGGCATAGTGACTGGGTTGAGTGAGGAGTTGGCCCAGCAGAACCAGATGAAGACATCGAATGTGGTC
TCACTGACGCAGGGGAAGCCGGCCGGAGGGCCTTTGGGGTGTCCACTGCAGAAAGGGA
mm4__chr7 1.30 Mbp_chr7_1302238 TGAAGCCTGGGAGAAACCACGTGGAGGCCCAGAGACGTGGGATCGAGAGGAGAGAGCCGCACCCTGGAAGGGCCCCGGCC
CGTCTGCCCATCCCTCTGTCCAGGCCGTTATCCTC
mm4__chr2 74.83 Mbp_chr2_74834098 GCACGTCACTGATCCGGAGGCCCGCGGGCCGGCAGCCCCTCAATAAGCCACATTGTTGCATGAAACTCCGGC
mm4__chr2 17.88 Mbp_chr2_17879298 CGTTCCTTCCCTTCCTCGAGAGGAGGCGCTTTCCTGGCGCTTGTAAATGCTTCCCGGGCCGTCTGCTCCGCCTTCTTTGT
CATTCCTCCCCTCCCCTTT
mm4__chr19 12.99 Mbp_chr19_12985893 GAGATCTGATGCCAGCTGGGGCACAGAACCTCCTCCCTCCATCTTCCCCTCCATAGGCTGGGTTTGGAGGAGTGGCCTCT
AGATGCAGC
mm4__chrX 120.47 Mbp_chrX_120468904 TACCGGCCCCTTCTTACTCTCCTGGGCCCTTCTTCCCCGCCATCCGTCGGTCAGGCCTTCTCTAGGTCACCGCTTGCCTC
CCCGGGCCAACCGCCGCCGCCCAGTGTTAGCTGCTGAGCCT
mm4__chr1 10.83 Mbp_chr1_10834501 GCCAGTGAATCCAGGGAGACTGAGGCTAGAGCAGGGAAGGAGGGGAACACGGGCGGGGGGGGGTGGCACTGCATGGTATA
mm4__chr7 75.05 Mbp_chr7_75052694 CTGCAGTGACGTCAGGTGACTCTACTCTTAGCATCCATTCTCAAGACCTCCCCGGCCAGCCTTACCCTCCATGGGAAATA
CTGGTCCTCCAGGCGACCGATTCCCAGGCACCCTCGGATGTTCTTGCCCCAT
mm4__chrX 108.30 Mbp_chrX_108296282 GTTCACTCTGTCAAACCCAACAGATAAGCTCTGTTCATGCTAATATTTGGCCACAGGCCCACTTCTCCTCCATACCTAGG
CAAGGAGGTATTGGGATGATTAATGGGTGAGAATTGCTACTCCTTCCCTTTCGTGGTCC
mm4__chr8 9.33 Mbp_chr8_9329565 TGTTCAGTCAGCACGCTAGGGGTATTCAAGTAATTAGAAATGAACCTTGAGAAGGGATGGTGGGGAAAACGCCCCCAGGG
CAAGCAGTAGGTATGCTTTCAACTCGTGGAAACTGCTGT
mm4__chr7 21.90 Mbp_chr7_21900113 TGCATACCTCCACGGACTCTTCATGGAGGGTAAGACACCCCAAGGGGTAAGTGGGGAACCTTTTCTTACTCAGGTTCAGA
TCAGTGTTTGTCCTCTGCTTACTGTTTCTCAGCATCTCCTCACTCACACA
mm4__chr3 135.84 Mbp_chr3_135839633 CCCAGCCTCCAAACCAATGAGAACCTCCCTTTCTTCTCCCCCGGGCTCACATCCCCTCCATGTGCCCCAGGACTTGACCA
GTTGTTGCCCAAAAGCAAGCTATACCACCAGGTTGCCTCCTGAGATGCTGCTCTACCCCT
mm4__chr20 62.59 Mbp_chr20_62590947 ATTCTGGTTCCCTGAGCAGCAGCCCGGGGTGTCAGCAAAGTGTGGGGCATGGACCCCGTCCCCTCTTCCCCTGCATGCTG
GGGTTCCCATGTCCCCAAGTGCATTGC
mm4__chr2 239.28 Mbp_chr2_239275807 GGCAAGATAGAACGCCAGGACCCCTGCCTTCCAGGAGCTCACAGGGAGGGGAAGACACCCAGGGGAGCCCCAGATAGCTC
CTCATGGTGCCACGTGCCACCCTGAAGGTATGGGGCGGAGAAGATG
mm4__chr19 40.27 Mbp_chr19_40266161 CCACTGCTTCGTCTCAGGTTCCCAGAACGGAGGGGAGGAGGGCGCGAGGCAGCGCCAGCTGGGCCCCTACCTGGAGCCTG
GGGCCTGGCCAGGGTCCACAGAGACCAAGGCGCCTTT
mm4__chr18 76.67 Mbp_chr18_76674719 CAGGTCTGAATCCCTGCTGGCTGCATCTGCAGGCTGGAGGGGAAGTGGGCCCTGGGTGCAGGACCCCTGCAGGAGGCCAT
CACAGGGTCCCTCCTGGGGCTCCAGGCAGGAGGAGCCACAGGCTCTCAAGGTCG
mm4__chr17 44.91 Mbp_chr17_44910383 ACAGGGCAGATGTCAAGCTCTCACCCAGTTCTGCTGTCGAATGGGGTGCAGGGAGGGGAAGAGGGACCAGGGCCTAGCAG
GACAGGGGCAGCTGCAAGCCCCACCTAGAAGTACCC
mm4__chr16 88.99 Mbp_chr16_88993393 CCTCACAGCCTCAGCACTTGCTTCCCCCTGCCGGGAGCACCATCCCCAGGGGCCCCAGGACTCCTTCCCCTCCATCCCAA
CACGTGGTTGGGACTAAACAATATGTTTGCAACTTGTTTTGGGGTTCACATGAGGGTTCC
mm4__chr16 2.97 Mbp_chr16_2973004 TCAGGTTGGGTCTAGGGTGTCCTCAAACAGGCTGAGGAGGTTCCGAGGCTCAAAGGAGGGGAAGGAGCCCCGAGGAGGCT
CTGAGTTGATGTCACTTAGGTCCAGGGCATCC
mm4__chr15 69.39 Mbp_chr15_69393672 GGAGAGAACCACCTTCCCCTCCAGGGAGGGGAAGACCTCCTGGGGGAGACCAGGAACCTCAGTATTTGCTTGACAGTTAT
CTGATGCTTGCCCGAAAATCAGGGTTGA
mm4__chr12 52.18 Mbp_chr12_52180838 TGAGGGCTTTGATTGGGCATAAAGGAGAGTAGGATATCTGGAGGGAAAGAGGGCCCAGGGCCCATGAGGAAGGAGCCCCT
GGGGCAGGCTGGGCAGGCTACCTTCTTC
mm4__chr12 52.00 Mbp_chr12_52003254 GCCCCTCATTCCTGACAGACCAGAGAGCCAGGGCCCCAGGCCCTCTTCCCCCCCACCTCCCCAGCTTAGGAGCAGTGACA
GTACCCA
mm4__chr11 40.29 Mbp_chr11_40293455 CAGATCCTCCAGGTGTTGCAGAACTCCGCGGTCGGGGCCGGGATCACGGGGGAGACGCAGGATGCGGTCCCTCGCCGGTG
GCCCCTGCCAGCCCCCGGGTCCCCTTCTCCTCCATCAGCCGGTAGTGGCTCCGGGAG
mm4__chr1 230.87 Mbp_chr1_230868140 CTTTTCCAGCCCCTACCAGCTGGGGCGGCCTCACGCCGGCAGGTGCCCACCGGGCCGGGGCGCCTCGGGGCGCCGGGAGG
GGAAGAGGGTCCGCGGCCAGGCCCGCACCTACCTCGTCCCCGAAGCGCACCACCTTCTGG
mm3__chr5 138.25 Mbp_chr5_138252935 CTGCTGCTGTCCTTTCCTCACCCCTTGTGGGCTGCAAGTCCACCTGGGTGTGGTGGAGGGGAAGAGGGCCCTGGGGAAGT
CCAGCTACCATAGGCTCAGGAGCAGAATCAAGGGAAGCGTGCA

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

Version 4.7 - Documentation  - Contact us - Downloads/local installation - Paper - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.