CRISPOR

Micro-homology scoring of potential deletions

Guide: GCCGGAAGTGGCCAGGGCGG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	(Wild-type sequence)
741.0	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCG------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`6 bp deleted → no frameshift`
362.0	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGG--GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`2 bp deleted → frameshift`
344.4	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGG---CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`3 bp deleted → no frameshift`
329.4	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGG------------CTCACAGCGCGCCCGGCTATTCTCGCAG`	`12 bp deleted → no frameshift`
329.4	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC------------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`12 bp deleted → no frameshift`
311.6	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGG-----GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`5 bp deleted → frameshift`
283.2	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC---------------TCACAGCGCGCCCGGCTATTCTCGCAG`	`15 bp deleted → no frameshift`
282.0	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGG-------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`7 bp deleted → frameshift`
230.8	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGG-----------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`11 bp deleted → frameshift`
218.4	`GCGGCCTAAGGACTCGGCG--------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`26 bp deleted → frameshift`
210.0	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC---------------------TCACAGCGCGCCCGGCTATTCTCGCAG`	`21 bp deleted → no frameshift`
199.8	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCG----------------------CGCCCGGCTATTCTCGCAG`	`22 bp deleted → frameshift`
191.4	`GCGGCCTAAGGACTCGGC-----------------------------------TCACAGCGCGCCCGGCTATTCTCGCAG`	`35 bp deleted → frameshift`
188.8	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC---------------TCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`15 bp deleted → no frameshift`
180.6	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCG------------------------CCCGGCTATTCTCGCAG`	`24 bp deleted → no frameshift`
180.6	`GCGGCCTAAGGACTCGGCGCG------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`24 bp deleted → no frameshift`
179.6	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGG----------------CTCACAGCGCGCCCGGCTATTCTCGCAG`	`16 bp deleted → frameshift`
170.8	`GCGGCCTAAGGACTCGGCGCGCCGG-----------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`17 bp deleted → frameshift`
170.8	`GCGGCCTAAGGACTCGGCGCGCC------------------------------------------CGGCTATTCTCGCAG`	`42 bp deleted → no frameshift`
162.8	`GCGGCCTAAGGACTCGGCGCGCCGG------------------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`18 bp deleted → no frameshift`
155.4	`GCGGCCTAAGGACTCGGCGCGCCGG---------------------------CTCACAGCGCGCCCGGCTATTCTCGCAG`	`27 bp deleted → no frameshift`
150.5	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAG------------------------CGCGCCCGGCTATTCTCGCAG`	`24 bp deleted → no frameshift`
148.2	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGG----------------------------CTATTCTCGCAG`	`28 bp deleted → frameshift`
140.0	`GCGGCCTAAGGACTCGGCGCGCCG---------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`21 bp deleted → no frameshift`
133.2	`GCGGCCTAAGGACTCGGCGCGC----------------------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`22 bp deleted → frameshift`
127.2	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC-------------------------------TATTCTCGCAG`	`31 bp deleted → frameshift`
121.2	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC--------------------------------CGGCTATTCTCGCAG`	`32 bp deleted → frameshift`
114.8	`GCGGCCTAAGGACTCGGCGCGCC-------------------------TCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`25 bp deleted → frameshift`
114.8	`GCGGCCTAAGGACTCGG-------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`25 bp deleted → frameshift`
110.4	`GCGGCCTAAGGACTCGGCGCGCCGGA--------------------CCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`20 bp deleted → frameshift`
109.2	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC--------------------------CCGGCTATTCTCGCAG`	`26 bp deleted → frameshift`
108.0	`GCGGCCTAAGGACTCGGCGCGC----------------------------------------GCCCGGCTATTCTCGCAG`	`40 bp deleted → frameshift`
99.9	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCA----------------------CAGCGCGCCCGGCTATTCTCGCAG`	`22 bp deleted → frameshift`
98.8	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC----------------------------GCGCCCGGCTATTCTCGCAG`	`28 bp deleted → frameshift`
94.2	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC-------------------------------------TATTCTCGCAG`	`37 bp deleted → frameshift`
94.0	`GCGGCCTAAGGACTCG-----------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`29 bp deleted → frameshift`
92.8	`GCGGCCTAAGGACTCGGCGCGCCGG-------------------------------------------CTATTCTCGCAG`	`43 bp deleted → frameshift`
91.5	`GCGGCCTAAGGAC----------------------------------CTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`34 bp deleted → frameshift`
89.2	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC------------------------------GCCCGGCTATTCTCGCAG`	`30 bp deleted → no frameshift`
89.2	`GCGGCCTAAGGACTCGGCGCG------------------------------GCTCACAGCGCGCCCGGCTATTCTCGCAG`	`30 bp deleted → no frameshift`
88.8	`GCGGCCTAAGGACTCGGCGC--------------------------------------------CCGGCTATTCTCGCAG`	`44 bp deleted → frameshift`
85.2	`GCGGC---------------------------------------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`39 bp deleted → no frameshift`
84.8	`GCGGCCTAAGGACTCGGCGCGC-------------------------------TCACAGCGCGCCCGGCTATTCTCGCAG`	`31 bp deleted → frameshift`
84.8	`GCGGCCTAAGG-------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`31 bp deleted → frameshift`
80.8	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGG--------------------------------CTATTCTCGCAG`	`32 bp deleted → frameshift`
80.8	`GCGGCCTAAGGACTCGGCG--------------------------------GCTCACAGCGCGCCCGGCTATTCTCGCAG`	`32 bp deleted → frameshift`
80.8	`GCGGCCTAAGG--------------------------------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`32 bp deleted → frameshift`
76.8	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC---------------------------------GGCTATTCTCGCAG`	`33 bp deleted → no frameshift`
76.8	`GCGGCCTAAGGACTCGGCGC---------------------------------TCACAGCGCGCCCGGCTATTCTCGCAG`	`33 bp deleted → no frameshift`
73.2	`GCG------------------------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`42 bp deleted → no frameshift`
72.8	`GCGGC------------------------------------------------TCACAGCGCGCCCGGCTATTCTCGCAG`	`48 bp deleted → no frameshift`
67.5	`GCGGCCTAAGGACTC----------------------------------------ACAGCGCGCCCGGCTATTCTCGCAG`	`40 bp deleted → frameshift`
63.6	`GCGGCCTAAGGACTCGGCGCGCCGGAAG-------------------------------CGCGCCCGGCTATTCTCGCAG`	`31 bp deleted → frameshift`
62.8	`GCGGCCTAAGGACTCGGCGCGCCG-------------------------------------CGCCCGGCTATTCTCGCAG`	`37 bp deleted → frameshift`
62.4	`GCGGCCTAAGGACTCGGC---------------------------------------------------TATTCTCGCAG`	`51 bp deleted → no frameshift`
61.0	`GCGGCCT------------------------------------------CGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`42 bp deleted → no frameshift`
60.0	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCG--------------------------------------CAG`	`38 bp deleted → frameshift`
60.0	`GCGGCCTAAGGACTCGGCGCGC--------------------------------------GCGCCCGGCTATTCTCGCAG`	`38 bp deleted → frameshift`
60.0	`GCGG--------------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAG`	`38 bp deleted → frameshift`
56.8	`GCGGCCTAAGGACTCGGCGCGCCG---------------------------------------CCCGGCTATTCTCGCAG`	`39 bp deleted → no frameshift`
54.0	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC----------------------------------------AG`	`40 bp deleted → frameshift`
52.5	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAG---------------------------------------------`	`45 bp deleted → no frameshift`
51.6	`GCGGCCTAAGG-----------------------------------------CTCACAGCGCGCCCGGCTATTCTCGCAG`	`41 bp deleted → frameshift`
42.0	`GCGGCCTAAGGACTCG---------------------------------------------CGCCCGGCTATTCTCGCAG`	`45 bp deleted → no frameshift`
40.0	`GCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC----------------------------------------------AG`	`46 bp deleted → frameshift`
40.0	`GCGGCCTAAGGACTCGGCGCG----------------------------------------------GCTATTCTCGCAG`	`46 bp deleted → frameshift`
40.0	`GCGGCCTAAGGACTCGGC----------------------------------------------CCGGCTATTCTCGCAG`	`46 bp deleted → frameshift`
38.0	`GCGGCCTAAGGACTCGGCGCGC-----------------------------------------------TATTCTCGCAG`	`47 bp deleted → frameshift`
38.0	`GCGGCCTAAGGACTCG-----------------------------------------------CCCGGCTATTCTCGCAG`	`47 bp deleted → frameshift`
36.6	`GCGGCCTAAGGACTCGGCGCGC--------------------------------------------------------AG`	`56 bp deleted → frameshift`
36.4	`GCGGCCTAAGGACTCGGCG------------------------------------------------GCTATTCTCGCAG`	`48 bp deleted → no frameshift`
34.4	`GCGGCCTAAGGACTCGGCGC-------------------------------------------------TATTCTCGCAG`	`49 bp deleted → frameshift`
33.3	`GCGGCCTAAGGAC--------------------------------------------AGCGCGCCCGGCTATTCTCGCAG`	`44 bp deleted → frameshift`
33.0	`GCGGCCTAAGGACTCGGCGC----------------------------------------------------------AG`	`58 bp deleted → frameshift`
33.0	`GCG----------------------------------------------------------CGCCCGGCTATTCTCGCAG`	`58 bp deleted → frameshift`
32.9	`GCGGCCTAAGGACTCG-------------------------------------------------------------CAG`	`61 bp deleted → frameshift`
32.8	`GCGGC----------------------------------------------------------------TATTCTCGCAG`	`64 bp deleted → frameshift`
31.2	`GCGGCC-----------------------------------------------------------CGGCTATTCTCGCAG`	`59 bp deleted → frameshift`
31.2	`GC---------------------------------------------------TCACAGCGCGCCCGGCTATTCTCGCAG`	`51 bp deleted → no frameshift`
30.0	`GCG------------------------------------------------------------CCCGGCTATTCTCGCAG`	`60 bp deleted → no frameshift`
28.5	`GCGGCCT-----------------------------------------------CACAGCGCGCCCGGCTATTCTCGCAG`	`47 bp deleted → frameshift`
28.4	`GCGGCCTAAGGACTCGGCGCGCCG-----------------------------------------------------CAG`	`53 bp deleted → frameshift`
25.8	`GCGGCCTAAG-------------------------------------------------CGCGCCCGGCTATTCTCGCAG`	`49 bp deleted → frameshift`
25.6	`GCGGC-------------------------------------------------------GCGCCCGGCTATTCTCGCAG`	`55 bp deleted → frameshift`
23.2	`GCGGCCTAAGG---------------------------------------------------------CTATTCTCGCAG`	`57 bp deleted → no frameshift`
23.2	`GCGGC---------------------------------------------------------GCCCGGCTATTCTCGCAG`	`57 bp deleted → no frameshift`
22.2	`GCGGCCTAAGGACTCGGCGCGCCGGAAG----------------------------------------------------`	`52 bp deleted → frameshift`
20.0	`GCGGCCTAAGGACTCGGC------------------------------------------------------------AG`	`60 bp deleted → no frameshift`
20.0	`GCGGCC------------------------------------------------------------GGCTATTCTCGCAG`	`60 bp deleted → no frameshift`
18.3	`GCGGCCTAAGGACT--------------------------------------------------------ATTCTCGCAG`	`56 bp deleted → frameshift`
18.0	`GC--------------------------------------------------------------CCGGCTATTCTCGCAG`	`62 bp deleted → frameshift`
17.2	`GCGGCCTA---------------------------------------------------------------TTCTCGCAG`	`63 bp deleted → no frameshift`
15.6	`GCGGCCTAAGGACTC-----------------------------------------------------------TCGCAG`	`59 bp deleted → frameshift`
14.0	`GC-------------------------------------------------------------------TATTCTCGCAG`	`67 bp deleted → frameshift`
10.4	`GCGGC-------------------------------------------------------------------------AG`	`73 bp deleted → frameshift`
10.0	`GCG--------------------------------------------------------------------------CAG`	`74 bp deleted → frameshift`
9.9	`GCGGCCT--------------------------------------------------------------------CGCAG`	`68 bp deleted → frameshift`
9.0	`GCGGCCTAAG----------------------------------------------------------------------`	`70 bp deleted → frameshift`
8.8	`GC----------------------------------------------------------------------------AG`	`76 bp deleted → frameshift`

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