CRISPOR

Micro-homology scoring of potential deletions

Guide: GGAGGGGAAGACGGCCCGGG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	(Wild-type sequence)
516.6	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGG---CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`3 bp deleted → no frameshift`
427.0	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG-----------------CGAAGCCGGCCTTGCACATGCCGGA`	`17 bp deleted → frameshift`
382.8	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC---------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`9 bp deleted → no frameshift`
362.0	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGG--GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`2 bp deleted → frameshift`
327.6	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGG----CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`4 bp deleted → frameshift`
269.4	`TGCCTGGGGCGCCCCACGATGGAGGGG----------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`16 bp deleted → frameshift`
268.0	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG--------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`8 bp deleted → frameshift`
256.2	`TGCCTGGGGCGCCCCACGATGGAGGG-----------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`17 bp deleted → frameshift`
242.8	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG----------TCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`10 bp deleted → frameshift`
229.6	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGG-------------------------CCTTGCACATGCCGGA`	`25 bp deleted → frameshift`
223.0	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC------------------------------TTGCACATGCCGGA`	`30 bp deleted → no frameshift`
208.8	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG-------------CCCGCGAAGCCGGCCTTGCACATGCCGGA`	`13 bp deleted → frameshift`
188.8	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACG---------------TCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`15 bp deleted → no frameshift`
188.8	`TGCCTGGGGCGCCCCACGATGGAGGGG---------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`15 bp deleted → no frameshift`
179.6	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCC----------------CGCGAAGCCGGCCTTGCACATGCCGGA`	`16 bp deleted → frameshift`
163.8	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC--------------------------GGCCTTGCACATGCCGGA`	`26 bp deleted → frameshift`
162.8	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC------------------GCGAAGCCGGCCTTGCACATGCCGGA`	`18 bp deleted → no frameshift`
162.8	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACG------------------CCCGCGAAGCCGGCCTTGCACATGCCGGA`	`18 bp deleted → no frameshift`
162.8	`TGCCTGGGGCGCCCCACGATGGAGG------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`18 bp deleted → no frameshift`
154.8	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCG-------------------AAGCCGGCCTTGCACATGCCGGA`	`19 bp deleted → frameshift`
147.2	`TGCCTGGGGCGCCCCACGATGG--------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`20 bp deleted → frameshift`
146.4	`TGCCTGGGGC----------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`34 bp deleted → frameshift`
140.0	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC---------------------GAAGCCGGCCTTGCACATGCCGGA`	`21 bp deleted → no frameshift`
140.0	`TGCCTGGGGCGCCCCACGATGG---------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`21 bp deleted → no frameshift`
135.0	`TGCCTGGGGCGCCC----------------------------------------GCGAAGCCGGCCTTGCACATGCCGGA`	`40 bp deleted → frameshift`
133.8	`TGCCTGGGGCGCCCCACGATGGAGGGGAAG------------------------------CCGGCCTTGCACATGCCGGA`	`30 bp deleted → no frameshift`
133.2	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACG----------------------CGAAGCCGGCCTTGCACATGCCGGA`	`22 bp deleted → frameshift`
120.4	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGG------------------------CCTTGCACATGCCGGA`	`24 bp deleted → no frameshift`
120.4	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACG------------------------AAGCCGGCCTTGCACATGCCGGA`	`24 bp deleted → no frameshift`
108.0	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGG----------------------------------------A`	`40 bp deleted → frameshift`
104.4	`TGCCTGGG-----------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`35 bp deleted → frameshift`
94.0	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCC-----------------------------TTGCACATGCCGGA`	`29 bp deleted → frameshift`
89.2	`TGCCTGGGGCGCCCCACG------------------------------TCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`30 bp deleted → no frameshift`
85.2	`TGCCTGGGGCGCCCC---------------------------------------GCGAAGCCGGCCTTGCACATGCCGGA`	`39 bp deleted → no frameshift`
80.8	`TGCCTGGGGCGC--------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`32 bp deleted → frameshift`
77.7	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGA---------------------------AGCCGGCCTTGCACATGCCGGA`	`27 bp deleted → no frameshift`
77.4	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCC-----------------------------------------GGA`	`41 bp deleted → frameshift`
76.8	`TGCCTGGGGCGCCCCACG---------------------------------CCCGCGAAGCCGGCCTTGCACATGCCGGA`	`33 bp deleted → no frameshift`
76.8	`TGCCTGGGG---------------------------------GCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`33 bp deleted → no frameshift`
71.0	`TGCCTGGGGCGCCCCACGA---------------------------------------AGCCGGCCTTGCACATGCCGGA`	`39 bp deleted → no frameshift`
70.5	`TGCCTGGGGCGCCCCA-----------------------------TCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`29 bp deleted → frameshift`
69.6	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGC-----------------------------------ACATGCCGGA`	`35 bp deleted → frameshift`
66.6	`TGCCTGGGGCGC--------------------------------------------GAAGCCGGCCTTGCACATGCCGGA`	`44 bp deleted → frameshift`
66.0	`TGCCTGG------------------------------------CATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`36 bp deleted → no frameshift`
63.0	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGG---------------------------------------------A`	`45 bp deleted → no frameshift`
62.8	`TGCCTGGGGCGCCCCACGATGGAGGGG-------------------------------------CCTTGCACATGCCGGA`	`37 bp deleted → frameshift`
62.8	`TGCCTGGGGCGCCCCACG-------------------------------------CGAAGCCGGCCTTGCACATGCCGGA`	`37 bp deleted → frameshift`
62.8	`TGCCTGGGGCG-------------------------------------TCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`37 bp deleted → frameshift`
60.0	`TGCCTGGGGCGCCCCACGATGGAGGG--------------------------------------CCTTGCACATGCCGGA`	`38 bp deleted → frameshift`
60.0	`TGCCTGGGGCGCCCC--------------------------------------CGCGAAGCCGGCCTTGCACATGCCGGA`	`38 bp deleted → frameshift`
60.0	`TGCCTGGGGCG----------------------------------------------AAGCCGGCCTTGCACATGCCGGA`	`46 bp deleted → frameshift`
56.8	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGG---------------------------------------A`	`39 bp deleted → no frameshift`
56.8	`TGCCTGGGGCGCCCCACGATGGAGG---------------------------------------CCTTGCACATGCCGGA`	`39 bp deleted → no frameshift`
54.6	`TGCCTGGGGCGCCCCACGAT--------------------------CGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`26 bp deleted → frameshift`
52.2	`TGCCTGGGGCGCCCCACGATGGA-----------------------------------AGCCGGCCTTGCACATGCCGGA`	`35 bp deleted → frameshift`
51.6	`TGCCTGGGGCGCC-----------------------------------------GCGAAGCCGGCCTTGCACATGCCGGA`	`41 bp deleted → frameshift`
51.6	`TGC-----------------------------------------ATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGA`	`41 bp deleted → frameshift`
51.6	`TGCCTGGGGCGCC-------------------------------------------------GGCCTTGCACATGCCGGA`	`49 bp deleted → frameshift`
51.6	`TGCC-------------------------------------------------CGCGAAGCCGGCCTTGCACATGCCGGA`	`49 bp deleted → frameshift`
49.5	`TGCCTGGGGCGCCCCACGATGGAG------------------------------------CCGGCCTTGCACATGCCGGA`	`36 bp deleted → no frameshift`
48.8	`TGCCTGGGGCGCCCCACGATGG------------------------------------------CCTTGCACATGCCGGA`	`42 bp deleted → no frameshift`
48.8	`TGCCTGGGGC------------------------------------------CCGCGAAGCCGGCCTTGCACATGCCGGA`	`42 bp deleted → no frameshift`
42.6	`TGCCTGGGGCGCC-----------------------------------------------------TTGCACATGCCGGA`	`53 bp deleted → frameshift`
42.0	`TGCCTGGGGCGCCCCACG---------------------------------------------GCCTTGCACATGCCGGA`	`45 bp deleted → no frameshift`
40.5	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGAC----------------------------------------ATGCCGGA`	`40 bp deleted → frameshift`
38.4	`TGCCTGGGGC-------------------------------------------------------CTTGCACATGCCGGA`	`55 bp deleted → frameshift`
38.0	`TGCCTGGGGCGCCCC-----------------------------------------------GGCCTTGCACATGCCGGA`	`47 bp deleted → frameshift`
37.0	`TGCCTGGGGCGCCCCACGATGGAGGGGA----------------------------------------------------`	`52 bp deleted → frameshift`
36.4	`TGCCTGGGGCGCCC------------------------------------------------GGCCTTGCACATGCCGGA`	`48 bp deleted → no frameshift`
33.0	`TGCC----------------------------------------------------------GGCCTTGCACATGCCGGA`	`58 bp deleted → frameshift`
32.8	`TGCC--------------------------------------------------GCGAAGCCGGCCTTGCACATGCCGGA`	`50 bp deleted → frameshift`
32.0	`TGCCTGGGGCGCCCCAC-------------------------------------------------------ATGCCGGA`	`55 bp deleted → frameshift`
31.5	`TGCCT--------------------------------------------------------------TGCACATGCCGGA`	`62 bp deleted → frameshift`
31.2	`TGCCTGGGGCGCCCC---------------------------------------------------TTGCACATGCCGGA`	`51 bp deleted → no frameshift`
31.2	`TGCCTGGGGC---------------------------------------------------CGGCCTTGCACATGCCGGA`	`51 bp deleted → no frameshift`
29.6	`TGCCTGGGGCGCCC----------------------------------------------------TTGCACATGCCGGA`	`52 bp deleted → frameshift`
29.6	`TGCCTGGGGCG----------------------------------------------------GCCTTGCACATGCCGGA`	`52 bp deleted → frameshift`
29.0	`TGCCTGGGGCGCCCCACGATGGA---------------------------------------------------------`	`57 bp deleted → no frameshift`
28.4	`TGCCTGGGGCGCCCCACGATGGAGGG-----------------------------------------------------A`	`53 bp deleted → frameshift`
28.4	`TGC-----------------------------------------------------GAAGCCGGCCTTGCACATGCCGGA`	`53 bp deleted → frameshift`
27.3	`TGCCTGGGGCGCCCCACGATG------------------------------------------------CACATGCCGGA`	`48 bp deleted → no frameshift`
26.8	`TGCCTGGGGCGCCCCACGATGGAGG------------------------------------------------------A`	`54 bp deleted → no frameshift`
26.8	`TGCCTGGGGCGCCCCACGATG------------------------------------------------------CCGGA`	`54 bp deleted → no frameshift`
25.8	`TGCCTGGGGCGCCCCACGATGGAGGGGAAGA-------------------------------------------------`	`49 bp deleted → frameshift`
24.6	`TGCCTGGGGCGCC----------------------------------------------------------------GGA`	`64 bp deleted → frameshift`
24.4	`TGCCTGGG--------------------------------------------------------CCTTGCACATGCCGGA`	`56 bp deleted → frameshift`
23.2	`TGCCTGG---------------------------------------------------------CCTTGCACATGCCGGA`	`57 bp deleted → no frameshift`
22.0	`TGCCTGGGGCGC----------------------------------------------------------ACATGCCGGA`	`58 bp deleted → frameshift`
20.0	`TGCCTGGGGCGCCCCACG------------------------------------------------------------GA`	`60 bp deleted → no frameshift`
20.0	`TGCCTGGGGC------------------------------------------------------------ACATGCCGGA`	`60 bp deleted → no frameshift`
18.2	`TGCC-------------------------------------------------------------------------GGA`	`73 bp deleted → frameshift`
18.0	`TGCCTGGGGCGCCCC--------------------------------------------------------------GGA`	`62 bp deleted → frameshift`
17.5	`TGC-------------------------------------------------------------------ACATGCCGGA`	`67 bp deleted → frameshift`
17.4	`TGCCTGGGGCGCCCCA---------------------------------------------------------TGCCGGA`	`57 bp deleted → no frameshift`
17.2	`TGCCTGGGGCGCCC---------------------------------------------------------------GGA`	`63 bp deleted → no frameshift`
14.8	`TGCCTGGGGC------------------------------------------------------------------CGGA`	`66 bp deleted → no frameshift`
14.1	`TGCCTGGGGCGCCCCACGA-------------------------------------------------------------`	`61 bp deleted → frameshift`
14.0	`TGCCTGGGGCG-------------------------------------------------------------------GA`	`67 bp deleted → frameshift`
12.9	`TGCCTG---------------------------------------------------------------CACATGCCGGA`	`63 bp deleted → no frameshift`
12.0	`TGCCTGGGG----------------------------------------------------------------------A`	`70 bp deleted → frameshift`
11.6	`TGCCTGGG-----------------------------------------------------------------------A`	`71 bp deleted → frameshift`
10.8	`TGCCTGG------------------------------------------------------------------------A`	`72 bp deleted → no frameshift`
9.6	`TGCCTG---------------------------------------------------------------------CCGGA`	`69 bp deleted → no frameshift`

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