CRISPOR

Micro-homology scoring of potential deletions

Guide: GCCGTCTTCCCCTCCATCGT GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	(Wild-type sequence)
325.6	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCC------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`18 bp deleted → no frameshift`
282.0	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCG-------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`7 bp deleted → frameshift`
256.2	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCC-----------------CAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`17 bp deleted → frameshift`
253.6	`CGGGCGACGATGCCCCCCGGGC-----------------------GCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`23 bp deleted → frameshift`
236.0	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCA---------------GGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`15 bp deleted → no frameshift`
232.2	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCC-------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`19 bp deleted → frameshift`
208.8	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCC-------------CCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`13 bp deleted → frameshift`
199.8	`CGGGCGACGATGCCCCCCGGG----------------------GCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`22 bp deleted → frameshift`
198.8	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCC--------------CAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`14 bp deleted → frameshift`
183.0	`CGGGCGACGATGCCCC----------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`34 bp deleted → frameshift`
179.6	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCC----------------CCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`16 bp deleted → frameshift`
172.2	`CGGGCGACGATGCCCCCCGGGCC-------------------------CCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`25 bp deleted → frameshift`
166.5	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCA----------------------GGTAGGGGAGCTGGCTGGGTGG`	`22 bp deleted → frameshift`
161.6	`CGGGCGACGATGCCCCCC--------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`32 bp deleted → frameshift`
153.6	`CGGGCGACGATGCCCCC---------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`33 bp deleted → no frameshift`
147.2	`CGGGCGACGATGCCCCCCGGGCCGTCTTCC--------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`20 bp deleted → frameshift`
140.0	`CGGGCGACGATGCCCCCCGGG---------------------GGCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`21 bp deleted → no frameshift`
135.0	`CGGGCG----------------------------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`40 bp deleted → frameshift`
133.2	`CGGGCGACGATGCCCCCCGGGCCG----------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`22 bp deleted → frameshift`
127.2	`CGGGCGACGATGCCCCCC-------------------------------CAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`31 bp deleted → frameshift`
121.2	`CGGGCGACGATGCCCCCCGGGC--------------------------------ACCAGGTAGGGGAGCTGGCTGGGTGG`	`32 bp deleted → frameshift`
120.4	`CGGGCGACGATGCCCCCCGG------------------------CGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`24 bp deleted → no frameshift`
116.1	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCA-------------------CCAGGTAGGGGAGCTGGCTGGGTGG`	`19 bp deleted → frameshift`
114.8	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCC-------------------------AGGTAGGGGAGCTGGCTGGGTGG`	`25 bp deleted → frameshift`
109.2	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCC--------------------------AGGTAGGGGAGCTGGCTGGGTGG`	`26 bp deleted → frameshift`
109.2	`CGGGCGACGATGCCCCCCGGGCC--------------------------CAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`26 bp deleted → frameshift`
105.0	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT---------------------AGGGGAGCTGGCTGGGTGG`	`21 bp deleted → no frameshift`
104.4	`CGGGCGACGATGCCC-----------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`35 bp deleted → frameshift`
103.6	`CGGGCGACGATGCCCCCCGGGCCGTCTTCC---------------------------AGGTAGGGGAGCTGGCTGGGTGG`	`27 bp deleted → no frameshift`
103.6	`CGGGCGACGATGCCCCCCGGGCC---------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`27 bp deleted → no frameshift`
103.6	`CGGGCGACGATGCCCCCCG---------------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`27 bp deleted → no frameshift`
89.2	`CGGGCGACGATGCCCCCC------------------------------CCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`30 bp deleted → no frameshift`
85.2	`CGGG---------------------------------------GCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`39 bp deleted → no frameshift`
80.8	`CGGGCGACGATGC--------------------------------GCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`32 bp deleted → frameshift`
76.8	`CGGGCGACGATGCCCCCCGG---------------------------------CACCAGGTAGGGGAGCTGGCTGGGTGG`	`33 bp deleted → no frameshift`
73.2	`CGGGCGACGATGCCCCCCGGGCC----------------------------------AGGTAGGGGAGCTGGCTGGGTGG`	`34 bp deleted → frameshift`
66.6	`CGGGCGACGATGCCCCCCGGG--------------------------------------------GAGCTGGCTGGGTGG`	`44 bp deleted → frameshift`
66.0	`CGGGCGACGATGCC------------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`36 bp deleted → no frameshift`
63.0	`CGGGCGACGATGCCCCCCGGG---------------------------------------------AGCTGGCTGGGTGG`	`45 bp deleted → no frameshift`
62.8	`CGGGCGACG-------------------------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`37 bp deleted → frameshift`
60.0	`CGGG--------------------------------------GGCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`38 bp deleted → frameshift`
56.8	`CGGGCGACGATGCCCCCCGGG---------------------------------------TAGGGGAGCTGGCTGGGTGG`	`39 bp deleted → no frameshift`
56.8	`CGGGCGACGATGCCCCCC---------------------------------------AGGTAGGGGAGCTGGCTGGGTGG`	`39 bp deleted → no frameshift`
54.0	`CGGGCGACGATGCCCCCCGG----------------------------------------TAGGGGAGCTGGCTGGGTGG`	`40 bp deleted → frameshift`
54.0	`CGGGCGACGATGCCCCC----------------------------------------AGGTAGGGGAGCTGGCTGGGTGG`	`40 bp deleted → frameshift`
51.6	`CGGGCGACGATGCCCC-----------------------------------------AGGTAGGGGAGCTGGCTGGGTGG`	`41 bp deleted → frameshift`
51.6	`CGGGCGACGATGC-----------------------------------------ACCAGGTAGGGGAGCTGGCTGGGTGG`	`41 bp deleted → frameshift`
51.6	`CGG-----------------------------------------CGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`41 bp deleted → frameshift`
51.6	`CGGGC-------------------------------------------------ACCAGGTAGGGGAGCTGGCTGGGTGG`	`49 bp deleted → frameshift`
49.5	`CGGGCGACGATGCCCCCCGGGCCGT------------------------------------AGGGGAGCTGGCTGGGTGG`	`36 bp deleted → no frameshift`
48.8	`CGGGCGACGATGCCC------------------------------------------AGGTAGGGGAGCTGGCTGGGTGG`	`42 bp deleted → no frameshift`
48.8	`CGGGC------------------------------------------CCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`42 bp deleted → no frameshift`
47.1	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCCT-------------------------------------GGCTGGGTGG`	`37 bp deleted → frameshift`
46.8	`CGGGCGACGATGCCCCCCGGGC---------------------------------------------------TGGGTGG`	`51 bp deleted → no frameshift`
46.4	`CGGGCGACGATGCCCCCCGGG-------------------------------------------GGAGCTGGCTGGGTGG`	`43 bp deleted → frameshift`
46.4	`CGGGCGACGATGCC-------------------------------------------AGGTAGGGGAGCTGGCTGGGTGG`	`43 bp deleted → frameshift`
45.0	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT--------------------------------------GG`	`38 bp deleted → frameshift`
44.4	`CG--------------------------------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTGG`	`44 bp deleted → frameshift`
40.0	`CGGGCGACGATGCCCCCCGG----------------------------------------------AGCTGGCTGGGTGG`	`46 bp deleted → frameshift`
38.7	`CGGGCGACGATGCCCCCCGGGCCGTCTTCCCCT-----------------------------------------GGGTGG`	`41 bp deleted → frameshift`
38.0	`CGGGCGACGATGCCCCCCGGGC-----------------------------------------------TGGCTGGGTGG`	`47 bp deleted → frameshift`
36.6	`CGGGCGACGATGCCCCCCGGG--------------------------------------------------------TGG`	`56 bp deleted → frameshift`
34.8	`CGGGCGACGATGCCCCCCGGGCCGTCT-------------------------------------------GGCTGGGTGG`	`43 bp deleted → frameshift`
32.8	`CGG--------------------------------------------------CACCAGGTAGGGGAGCTGGCTGGGTGG`	`50 bp deleted → frameshift`
29.6	`CGGGCGACGATGCCCCCCGG----------------------------------------------------CTGGGTGG`	`52 bp deleted → frameshift`
28.5	`CGGGCGACGATGCCCCCCGGGCCGTCT-----------------------------------------------GGGTGG`	`47 bp deleted → frameshift`
28.2	`CGGG-------------------------------------------------------------GAGCTGGCTGGGTGG`	`61 bp deleted → frameshift`
27.3	`CGGGCGAC------------------------------------------------CAGGTAGGGGAGCTGGCTGGGTGG`	`48 bp deleted → no frameshift`
27.0	`CGGG--------------------------------------------------------------AGCTGGCTGGGTGG`	`62 bp deleted → frameshift`
25.6	`CGGGCGACGATGCCCCCCGGG-------------------------------------------------------GTGG`	`55 bp deleted → frameshift`
24.4	`CGGGCGACGATGC--------------------------------------------------------TGGCTGGGTGG`	`56 bp deleted → frameshift`
24.4	`CGGG--------------------------------------------------------TAGGGGAGCTGGCTGGGTGG`	`56 bp deleted → frameshift`
23.2	`CGGGCGACGATGCCCCCCGG---------------------------------------------------------TGG`	`57 bp deleted → no frameshift`
23.2	`CGG---------------------------------------------------------TAGGGGAGCTGGCTGGGTGG`	`57 bp deleted → no frameshift`
21.3	`CGGGCGACGATGCCCCCCGGGCCGT-----------------------------------------------------GG`	`53 bp deleted → frameshift`
20.8	`CGGGCGACGATGCCCCCCGGG-----------------------------------------------------------`	`59 bp deleted → frameshift`
20.0	`CGGGCGACGATGCCCCCCGG------------------------------------------------------------`	`60 bp deleted → no frameshift`
20.0	`CGGGCGACGATGC------------------------------------------------------------TGGGTGG`	`60 bp deleted → no frameshift`
20.0	`CGGG------------------------------------------------------------GGAGCTGGCTGGGTGG`	`60 bp deleted → no frameshift`
19.8	`CGGGC--------------------------------------------------------------------TGGGTGG`	`68 bp deleted → frameshift`
17.4	`CGGGCGACGA---------------------------------------------------------GCTGGCTGGGTGG`	`57 bp deleted → no frameshift`
17.2	`CGG---------------------------------------------------------------AGCTGGCTGGGTGG`	`63 bp deleted → no frameshift`
16.4	`CGGGC----------------------------------------------------------------TGGCTGGGTGG`	`64 bp deleted → frameshift`
15.6	`CGGGCGACGATG-----------------------------------------------------------GCTGGGTGG`	`59 bp deleted → frameshift`
15.6	`CGGG-------------------------------------------------------------------------TGG`	`73 bp deleted → frameshift`
15.0	`CGGGCGA------------------------------------------------------------GCTGGCTGGGTGG`	`60 bp deleted → no frameshift`
12.9	`CGGGCGACGATG---------------------------------------------------------------GGTGG`	`63 bp deleted → no frameshift`
12.8	`CGG---------------------------------------------------------------------CTGGGTGG`	`69 bp deleted → no frameshift`
10.8	`CGGG------------------------------------------------------------------------GTGG`	`72 bp deleted → no frameshift`
10.5	`CGGGCGACGATG-------------------------------------------------------------------G`	`67 bp deleted → frameshift`
10.0	`CGG--------------------------------------------------------------------------TGG`	`74 bp deleted → frameshift`
8.8	`CGGG----------------------------------------------------------------------------`	`76 bp deleted → frameshift`
8.4	`CGG-----------------------------------------------------------------------------`	`77 bp deleted → frameshift`

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