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Contents:

PCR primers for off-targets of GGCTCCGGCATGTGCAAGGC CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529598_F TCGTCGGCAGCGTCCACGATGGAGGGGAAGACG 59.8 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529598_R GTCTCGTGGGCTCGGGCTATTCTCGCAGCTCACCA 60.1 1.00
mm1__chr9 100.73 Mbp_chr9_100731275_F TCGTCGGCAGCGTCGCCAGCTCACCATGGATGAT 60.1 0.92
mm1__chr9 100.73 Mbp_chr9_100731275_R GTCTCGTGGGCTCGGCTAGGGAGCTCCACGATGGA 60.4 0.92
mm1__chr5 98.34 Mbp_chr5_98338779_F TCGTCGGCAGCGTCAGCACGCTCCTCATCAACAA 59.9 0.92
mm1__chr5 98.34 Mbp_chr5_98338779_R GTCTCGTGGGCTCGGTTTTGACCCATGCCCACCAT 60.1 0.92
mm1__chr22 15.72 Mbp_chr22_15721522_F TCGTCGGCAGCGTCCCGTGCTCGTCATTGACAAC 59.8 0.92
mm1__chr22 15.72 Mbp_chr22_15721522_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.92
mm1__chr2 131.63 Mbp_chr2_131626174_F TCGTCGGCAGCGTCATACCGCTGTGCTCGTCATT 59.8 0.92
mm1__chr2 131.63 Mbp_chr2_131626174_R GTCTCGTGGGCTCGGCTTCTGATGCATGCCCTCCA 60.1 0.92
mm1__chr2 131.26 Mbp_chr2_131263591_F TCGTCGGCAGCGTCTGGATGATGATACCGCCGTG 59.9 0.92
mm1__chr2 131.26 Mbp_chr2_131263591_R GTCTCGTGGGCTCGGGATGGAAGGGAAGACAGCCC 60.1 0.92
mm1__chr2 130.66 Mbp_chr2_130656785_F TCGTCGGCAGCGTCATACCGCTGTGCTCGTCATT 59.8 0.92
mm1__chr2 130.66 Mbp_chr2_130656785_R GTCTCGTGGGCTCGGGATGGAAGGGAAGACAGCCC 60.1 0.92
mm1__chr2 130.46 Mbp_chr2_130463884_F TCGTCGGCAGCGTCGATGGAAGGGAAGACAGCCC 60.1 0.92
mm1__chr2 130.46 Mbp_chr2_130463884_R GTCTCGTGGGCTCGGCCGTGCTCGTCATTGACAAC 59.8 0.92
mm1__chr2 130.08 Mbp_chr2_130075312_F TCGTCGGCAGCGTCGATGGAAGGGAAGACAGCCC 60.1 0.92
mm1__chr2 130.08 Mbp_chr2_130075312_R GTCTCGTGGGCTCGGATACCGCTGTGCTCGTCATT 59.8 0.92
mm1__chr14 19.40 Mbp_chr14_19402849_F TCGTCGGCAGCGTCATCATGTTCTGGTGCCTGGG 60.0 0.92
mm1__chr14 19.40 Mbp_chr14_19402849_R GTCTCGTGGGCTCGGCCGTGCTCGTCATTGACAAC 59.8 0.92
mm1__chr14 19.00 Mbp_chr14_18998903_F TCGTCGGCAGCGTCCCGTGCTCGTCATTGACAAC 59.8 0.92
mm1__chr14 19.00 Mbp_chr14_18998903_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.92
mm1__chr1 223.86 Mbp_chr1_223864792_F TCGTCGGCAGCGTCCATGGAAGGGAAGATGGCCC 60.4 0.90
mm1__chr1 223.86 Mbp_chr1_223864792_R GTCTCGTGGGCTCGGGCCAGCTCACCATGGATGAT 60.1 0.90
mm2__chr22 41.08 Mbp_chr22_41075180_F TCGTCGGCAGCGTCATGCCCACCATCATGACCTG 60.1 0.83
mm2__chr22 41.08 Mbp_chr22_41075180_R GTCTCGTGGGCTCGGTATTGCTGCGCTCGTTGTTG 59.8 0.83
mm3__chr11 44.89 Mbp_chr11_44891604_F TCGTCGGCAGCGTCAAACCTTCAGGGAGGCAACC 60.1 0.73
mm3__chr11 44.89 Mbp_chr11_44891604_R GTCTCGTGGGCTCGGCAGTCCCACCAATCCTCACC 60.0 0.73
mm2__chr5 61.83 Mbp_chr5_61832986_F TCGTCGGCAGCGTCCCATCATCATGCCCTGGTGT 60.1 0.69
mm2__chr5 61.83 Mbp_chr5_61832986_R GTCTCGTGGGCTCGGTCATGGGCTGGTCATTGACA 59.3 0.69
mm2__chr20 1.16 Mbp_chr20_1160619_F TCGTCGGCAGCGTCGCTCCTCCACCGATCACAAT 59.8 0.69
mm2__chr20 1.16 Mbp_chr20_1160619_R GTCTCGTGGGCTCGGGGGAAACATGGTTCCAGGGG 60.6 0.69
mm2__chr1 237.93 Mbp_chr1_237926866_F TCGTCGGCAGCGTCCTCCTCCACCAGTCACGATG 59.8 0.69
mm2__chr1 237.93 Mbp_chr1_237926866_R GTCTCGTGGGCTCGGTGGAGGGAAACACAGCTTGG 60.1 0.69
mm2__chr1 103.57 Mbp_chr1_103569588_F Primer3: not found at this Tm N.d. 0.69
mm2__chr1 103.57 Mbp_chr1_103569588_R Primer3: not found at this Tm N.d. 0.69
mm1__chr17 81.51 Mbp_chr17_81512295_F TCGTCGGCAGCGTCCCATCCACTCACCTGGTGTC 60.0 0.69
mm1__chr17 81.51 Mbp_chr17_81512295_R GTCTCGTGGGCTCGGATGGAAGAAGAGATCGCCGC 60.2 0.69
mm3__chr5 15.45 Mbp_chr5_15450735_F Primer3: not found at this Tm N.d. 0.64
mm3__chr5 15.45 Mbp_chr5_15450735_R Primer3: not found at this Tm N.d. 0.64
mm2__chrX 53.14 Mbp_chrX_53143854_F TCGTCGGCAGCGTCGATGGAGGGGAACACAGCTC 60.1 0.64
mm2__chrX 53.14 Mbp_chrX_53143854_R GTCTCGTGGGCTCGGTGCTCTTCCACAGGTTGCAA 60.1 0.64
mm2__chr2 57.76 Mbp_chr2_57764988_F TCGTCGGCAGCGTCAGATTGCTGGCTCGTCAATGA 60.0 0.64
mm2__chr2 57.76 Mbp_chr2_57764988_R GTCTCGTGGGCTCGGCTTCTTGCCCATGCCCACTA 60.0 0.64
mm3__chr3 139.49 Mbp_chr3_139493850_F TCGTCGGCAGCGTCTGCCGGTCACAATGGAAGAA 59.8 0.51
mm3__chr3 139.49 Mbp_chr3_139493850_R GTCTCGTGGGCTCGGATGGAAGGGAACATGGCTCG 60.1 0.51
mm4__chr4 79.53 Mbp_chr4_79525832_F TCGTCGGCAGCGTCGCATCAGGCACAAGAAAGAGT 58.8 0.45
mm4__chr4 79.53 Mbp_chr4_79525832_R GTCTCGTGGGCTCGGACTGGTCTTGTCGAGGTCCT 60.1 0.45
mm2__chr5 6.57 Mbp_chr5_6573893_F Primer3: not found at this Tm N.d. 0.44
mm2__chr5 6.57 Mbp_chr5_6573893_R Primer3: not found at this Tm N.d. 0.44
mm4__chr1 202.87 Mbp_chr1_202873329_F TCGTCGGCAGCGTCCCTCGGCTGATCACAATGGA 59.8 0.43
mm4__chr1 202.87 Mbp_chr1_202873329_R GTCTCGTGGGCTCGGGATGGAGGGGAACATGGCTC 60.1 0.43
mm3__chrY 18.15 Mbp_chrY_18147919_F Primer3: not found at this Tm N.d. 0.36
mm3__chrY 18.15 Mbp_chrY_18147919_R Primer3: not found at this Tm N.d. 0.36
mm3__chrY 17.76 Mbp_chrY_17758066_F TCGTCGGCAGCGTCAACGGTGGAGGGAAACATGG 60.2 0.36
mm3__chrY 17.76 Mbp_chrY_17758066_R GTCTCGTGGGCTCGGTCCTCTGTCCCACTCCTCTG 59.9 0.36
mm4__chr8 42.42 Mbp_chr8_42416144_F TCGTCGGCAGCGTCCACCACCGCTACAGAGGATT 59.4 0.35
mm4__chr8 42.42 Mbp_chr8_42416144_R GTCTCGTGGGCTCGGGCAGGGAAGTGCAGGTCTTA 59.6 0.35
mm4__chr2 43.98 Mbp_chr2_43984752_F Primer3: not found at this Tm N.d. 0.35
mm4__chr2 43.98 Mbp_chr2_43984752_R Primer3: not found at this Tm N.d. 0.35
mm4__chrX 43.49 Mbp_chrX_43487251_F Primer3: not found at this Tm N.d. 0.34
mm4__chrX 43.49 Mbp_chrX_43487251_R Primer3: not found at this Tm N.d. 0.34
mm3__chr3 12.07 Mbp_chr3_12070260_F TCGTCGGCAGCGTCGCCATGCTGGTCATTGACAG 59.5 0.33
mm3__chr3 12.07 Mbp_chr3_12070260_R GTCTCGTGGGCTCGGGAGTCCTTCTGACCCATGCC 60.1 0.33
mm4__chr2 127.41 Mbp_chr2_127406608_F Primer3: not found at this Tm N.d. 0.32
mm4__chr2 127.41 Mbp_chr2_127406608_R Primer3: not found at this Tm N.d. 0.32
mm4__chr17 36.85 Mbp_chr17_36853715_F Primer3: not found at this Tm N.d. 0.32
mm4__chr17 36.85 Mbp_chr17_36853715_R Primer3: not found at this Tm N.d. 0.32
mm2__chr2 73.90 Mbp_chr2_73901350_F TCGTCGGCAGCGTCGTGTGAAGAGGAGACCACCG 60.0 0.31
mm2__chr2 73.90 Mbp_chr2_73901350_R GTCTCGTGGGCTCGGCCCACAATGGAGGGGAAGAC 60.0 0.31
mm4__chr16 1.14 Mbp_chr16_1144122_F Primer3: not found at this Tm N.d. 0.29
mm4__chr16 1.14 Mbp_chr16_1144122_R Primer3: not found at this Tm N.d. 0.29
mm4__chr16 1.14 Mbp_chr16_1144003_F TCGTCGGCAGCGTCGAAGCACCACGTCCAGAGAA 59.9 0.29
mm4__chr16 1.14 Mbp_chr16_1144003_R GTCTCGTGGGCTCGGCTGGCTGTGATGTGTGCAAG 59.7 0.29
mm4__chr9 124.32 Mbp_chr9_124315722_F TCGTCGGCAGCGTCGGTCCCTGGTCACACAGATG 60.0 0.28
mm4__chr9 124.32 Mbp_chr9_124315722_R GTCTCGTGGGCTCGGGGACCAAATCCCCTCTGCAG 60.3 0.28
mm4__chr6 86.48 Mbp_chr6_86475418_F Primer3: not found at this Tm N.d. 0.28
mm4__chr6 86.48 Mbp_chr6_86475418_R Primer3: not found at this Tm N.d. 0.28
mm3__chr5 57.48 Mbp_chr5_57482645_F TCGTCGGCAGCGTCGTCGAGGACGCCCTATCATG 60.0 0.28
mm3__chr5 57.48 Mbp_chr5_57482645_R GTCTCGTGGGCTCGGGAAGATCTCAGCCAGCACCA 59.7 0.28
mm4__chr10 49.98 Mbp_chr10_49977914_F TCGTCGGCAGCGTCAGTTGTTGGGAGTGGCTGAG 59.8 0.28
mm4__chr10 49.98 Mbp_chr10_49977914_R GTCTCGTGGGCTCGGGGCACAGGTAGACAAGGCAT 60.0 0.28
mm4__chr10 45.86 Mbp_chr10_45858151_F TCGTCGGCAGCGTCTAAGGCACAGGCAGACAAGG 59.9 0.28
mm4__chr10 45.86 Mbp_chr10_45858151_R GTCTCGTGGGCTCGGAGTTGTTGGGAGTGGCTGAG 59.8 0.28
mm4__chr16 1.14 Mbp_chr16_1144163_F TCGTCGGCAGCGTCCTTGCACACATCGCAGCC 59.8 0.27
mm4__chr16 1.14 Mbp_chr16_1144163_R GTCTCGTGGGCTCGGAAACCAGCCTTGCTCTACCC 59.9 0.27
mm4__chr6 14.50 Mbp_chr6_14501462_F TCGTCGGCAGCGTCTCATCCTCTGGCCTTTTCAGG 59.7 0.26
mm4__chr6 14.50 Mbp_chr6_14501462_R GTCTCGTGGGCTCGGACTCTCCATGCTGCATGTGA 59.3 0.26
mm4__chr8 143.38 Mbp_chr8_143380411_F TCGTCGGCAGCGTCCACTGGGAGCAGCTCATCC 60.1 0.26
mm4__chr8 143.38 Mbp_chr8_143380411_R GTCTCGTGGGCTCGGGCTCATCACAGCCACTTTCG 59.5 0.26
mm0__chr5 77.78 Mbp_chr5_77784922_F TCGTCGGCAGCGTCATATCACCGCGCTCGTCATT 59.9 0.25
mm0__chr5 77.78 Mbp_chr5_77784922_R GTCTCGTGGGCTCGGAACGATGGAGGGGAAGACTG 59.0 0.25
mm4__chr3 126.06 Mbp_chr3_126058096_F Primer3: not found at this Tm N.d. 0.25
mm4__chr3 126.06 Mbp_chr3_126058096_R Primer3: not found at this Tm N.d. 0.25
mm3__chr16 50.06 Mbp_chr16_50059172_F TCGTCGGCAGCGTCGAGGGGAACATGGTTCAGGG 60.0 0.25
mm3__chr16 50.06 Mbp_chr16_50059172_R GTCTCGTGGGCTCGGACTTCTCCACGATGGCGATG 60.1 0.25
mm4__chr15 88.54 Mbp_chr15_88540494_F Primer3: not found at this Tm N.d. 0.25
mm4__chr15 88.54 Mbp_chr15_88540494_R Primer3: not found at this Tm N.d. 0.25
mm4__chr13 38.68 Mbp_chr13_38679186_F Primer3: not found at this Tm N.d. 0.24
mm4__chr13 38.68 Mbp_chr13_38679186_R Primer3: not found at this Tm N.d. 0.24
mm4__chr1 169.96 Mbp_chr1_169960962_F Primer3: not found at this Tm N.d. 0.24
mm4__chr1 169.96 Mbp_chr1_169960962_R Primer3: not found at this Tm N.d. 0.24
mm4__chr7 45.65 Mbp_chr7_45651375_F TCGTCGGCAGCGTCTCTGTTGGGACCATCTTGGC 59.9 0.24
mm4__chr7 45.65 Mbp_chr7_45651375_R GTCTCGTGGGCTCGGAAAGTGTCCTCCTCTGCCTG 59.3 0.24
mm4__chr20 23.03 Mbp_chr20_23034826_F TCGTCGGCAGCGTCCTGAAATTCACCAAGCCGCC 60.1 0.24
mm4__chr20 23.03 Mbp_chr20_23034826_R GTCTCGTGGGCTCGGCCACTTGCCTTGCACTTGAC 59.9 0.24
mm1__chr15 43.99 Mbp_chr15_43990125_F TCGTCGGCAGCGTCAATGAAGGGGAAGATGGCCC 59.7 0.24
mm1__chr15 43.99 Mbp_chr15_43990125_R GTCTCGTGGGCTCGGGCCAGCTCACCATGGATGAT 60.1 0.24
mm4__chr2 49.98 Mbp_chr2_49982111_F Primer3: not found at this Tm N.d. 0.24
mm4__chr2 49.98 Mbp_chr2_49982111_R Primer3: not found at this Tm N.d. 0.24
mm4__chr14 61.53 Mbp_chr14_61529129_F TCGTCGGCAGCGTCGAGGGTCACTGTAAACTGGCA 59.9 0.23
mm4__chr14 61.53 Mbp_chr14_61529129_R GTCTCGTGGGCTCGGAGCGATATAGTCTGGCGTGC 60.0 0.23
mm2__chr5 80.30 Mbp_chr5_80302788_F TCGTCGGCAGCGTCATGATGAAGGGGAAGGCAGC 60.1 0.22
mm2__chr5 80.30 Mbp_chr5_80302788_R GTCTCGTGGGCTCGGAGCTCACCATGCATGACGAT 59.8 0.22
mm3__chr16 28.57 Mbp_chr16_28573983_F Primer3: not found at this Tm N.d. 0.21
mm3__chr16 28.57 Mbp_chr16_28573983_R Primer3: not found at this Tm N.d. 0.21
mm4__chr2 91.59 Mbp_chr2_91588358_F TCGTCGGCAGCGTCGCAGACACAGTCTCCTTCCG 60.3 0.20
mm4__chr2 91.59 Mbp_chr2_91588358_R GTCTCGTGGGCTCGGGCAGAGGAGCTGGAATGAGG 60.1 0.20
mm4__chr1_KI270712v1_random 131.34 Kbp_chr1_KI270712v1_random_131344_F TCGTCGGCAGCGTCACTTAATGGCACCCGAGCAG 60.3 0.20
mm4__chr1_KI270712v1_random 131.34 Kbp_chr1_KI270712v1_random_131344_R GTCTCGTGGGCTCGGCATCTCCACCAGCCCCTTC 59.7 0.20
mm4__chr1 144.79 Mbp_chr1_144787693_F TCGTCGGCAGCGTCCATCTCCACCAGCCCCTTC 59.7 0.20
mm4__chr1 144.79 Mbp_chr1_144787693_R GTCTCGTGGGCTCGGACTTAATGGCACCCGAGCAG 60.3 0.20
mm4__chr1 144.18 Mbp_chr1_144178671_F TCGTCGGCAGCGTCACTTAATGGCACCCGAGCAG 60.3 0.20
mm4__chr1 144.18 Mbp_chr1_144178671_R GTCTCGTGGGCTCGGCATCTCCACCAGCCCCTTC 59.7 0.20
mm4__chr1 143.35 Mbp_chr1_143353086_F TCGTCGGCAGCGTCACTTAATGGCACCCGAGCAG 60.3 0.20
mm4__chr1 143.35 Mbp_chr1_143353086_R GTCTCGTGGGCTCGGCATCTCCACCAGCCCCTTC 59.7 0.20
mm4__chr1 194.30 Mbp_chr1_194299168_F TCGTCGGCAGCGTCAGACAGTTGGTGAGCAATAGGA 59.0 0.19
mm4__chr1 194.30 Mbp_chr1_194299168_R GTCTCGTGGGCTCGGTTCCATGTGACAACGCCTGA 59.8 0.19
mm4__chr19 10.61 Mbp_chr19_10608580_F Primer3: not found at this Tm N.d. 0.18
mm4__chr19 10.61 Mbp_chr19_10608580_R Primer3: not found at this Tm N.d. 0.18
mm4__chr7 80.58 Mbp_chr7_80578924_F TCGTCGGCAGCGTCCCTGGAAGCAACTGAAATTTCA 57.4 0.18
mm4__chr7 80.58 Mbp_chr7_80578924_R GTCTCGTGGGCTCGGGGGTCCAAGCTTCTCACTGT 59.6 0.18
mm4__chr4 112.90 Mbp_chr4_112903799_F Primer3: not found at this Tm N.d. 0.17
mm4__chr4 112.90 Mbp_chr4_112903799_R Primer3: not found at this Tm N.d. 0.17
mm3__chr19 30.42 Mbp_chr19_30423754_F TCGTCGGCAGCGTCGTCTGCTCAATGTGTCCCCA 59.9 0.17
mm3__chr19 30.42 Mbp_chr19_30423754_R GTCTCGTGGGCTCGGGCCACAGCTGCTGTCACT 60.2 0.17
mm3__chr3 4.49 Mbp_chr3_4492381_F TCGTCGGCAGCGTCCATTTGAGGCAAGACAGGGC 59.4 0.17
mm3__chr3 4.49 Mbp_chr3_4492381_R GTCTCGTGGGCTCGGTTCACCCGCTGCCAAGTAAT 59.9 0.17
mm4__chr16 52.74 Mbp_chr16_52741230_F TCGTCGGCAGCGTCAGAATTCCCTCAGAAATCAGACCA 59.4 0.17
mm4__chr16 52.74 Mbp_chr16_52741230_R GTCTCGTGGGCTCGGGCTGGAGAAAATAAATTCTTAGCCA 57.9 0.17
mm4__chr12 66.64 Mbp_chr12_66642304_F Primer3: not found at this Tm N.d. 0.16
mm4__chr12 66.64 Mbp_chr12_66642304_R Primer3: not found at this Tm N.d. 0.16
mm4__chr2 46.15 Mbp_chr2_46145147_F TCGTCGGCAGCGTCAGAAGGTCACTGCAAGCTGG 60.2 0.16
mm4__chr2 46.15 Mbp_chr2_46145147_R GTCTCGTGGGCTCGGGTCAGGAGTCCCACAGAACG 60.0 0.16
mm2__chr18 62.44 Mbp_chr18_62442044_F Primer3: not found at this Tm N.d. 0.16
mm2__chr18 62.44 Mbp_chr18_62442044_R Primer3: not found at this Tm N.d. 0.16
mm4__chr20 17.96 Mbp_chr20_17960048_F Primer3: not found at this Tm N.d. 0.15
mm4__chr20 17.96 Mbp_chr20_17960048_R Primer3: not found at this Tm N.d. 0.15
mm4__chr20 16.57 Mbp_chr20_16573540_F TCGTCGGCAGCGTCATCCATGCGCCACGGTTT 60.3 0.15
mm4__chr20 16.57 Mbp_chr20_16573540_R GTCTCGTGGGCTCGGATAACCTCTGCGCCAAGGTC 60.1 0.15
mm3__chr11 1.80 Mbp_chr11_1804036_F TCGTCGGCAGCGTCCACGATGGAGGGGAAGACG 59.8 0.15
mm3__chr11 1.80 Mbp_chr11_1804036_R GTCTCGTGGGCTCGGCCCAGCTCACCATGGATGAT 59.5 0.15
mm4__chr18 46.53 Mbp_chr18_46528927_F TCGTCGGCAGCGTCATCCAGCCAACAACCTCGAG 60.0 0.15
mm4__chr18 46.53 Mbp_chr18_46528927_R GTCTCGTGGGCTCGGCTCCTAGCTGATGCACCCAC 60.1 0.15
mm4__chr12 126.05 Mbp_chr12_126049792_F Primer3: not found at this Tm N.d. 0.14
mm4__chr12 126.05 Mbp_chr12_126049792_R Primer3: not found at this Tm N.d. 0.14
mm4__chr10 127.32 Mbp_chr10_127318490_F TCGTCGGCAGCGTCTAACCATGTGTGCAGGAGGC 60.3 0.14
mm4__chr10 127.32 Mbp_chr10_127318490_R GTCTCGTGGGCTCGGAAGCAGTCATCAGAGTGGCC 60.0 0.14
mm4__chr22 32.63 Mbp_chr22_32631877_F Primer3: not found at this Tm N.d. 0.14
mm4__chr22 32.63 Mbp_chr22_32631877_R Primer3: not found at this Tm N.d. 0.14
mm4__chr6 159.44 Mbp_chr6_159443588_F TCGTCGGCAGCGTCGAGACTTCATAGCCACTGGGG 59.8 0.12
mm4__chr6 159.44 Mbp_chr6_159443588_R GTCTCGTGGGCTCGGTAGACCTGGAAAGCTTCGGC 59.7 0.12
mm4__chr7 44.44 Mbp_chr7_44444136_F TCGTCGGCAGCGTCCGAAGGTTTGCTGTATTGAGAACA 59.7 0.11
mm4__chr7 44.44 Mbp_chr7_44444136_R GTCTCGTGGGCTCGGAGAAGGTTAAAGTGGCGGGC 60.6 0.11
mm4__chr14 78.28 Mbp_chr14_78281868_F Primer3: not found at this Tm N.d. 0.11
mm4__chr14 78.28 Mbp_chr14_78281868_R Primer3: not found at this Tm N.d. 0.11
mm4__chr18 37.78 Mbp_chr18_37778138_F TCGTCGGCAGCGTCCTGACGTCCACTTTAGCCTGT 59.9 0.10
mm4__chr18 37.78 Mbp_chr18_37778138_R GTCTCGTGGGCTCGGCTGAGGCTGGCTCTGGAAAA 59.9 0.10
mm4__chr15 69.08 Mbp_chr15_69083040_F Primer3: not found at this Tm N.d. 0.10
mm4__chr15 69.08 Mbp_chr15_69083040_R Primer3: not found at this Tm N.d. 0.10
mm4__chr6 36.35 Mbp_chr6_36349440_F Primer3: not found at this Tm N.d. 0.10
mm4__chr6 36.35 Mbp_chr6_36349440_R Primer3: not found at this Tm N.d. 0.10
mm4__chr21 45.18 Mbp_chr21_45179183_F TCGTCGGCAGCGTCGTCACAGCCTCAGTTCCTCG 60.3 0.10
mm4__chr21 45.18 Mbp_chr21_45179183_R GTCTCGTGGGCTCGGCTTTGGTGGCAGGGATCCAT 60.0 0.10
mm4__chr6 159.98 Mbp_chr6_159980553_F TCGTCGGCAGCGTCTAAGAAGGTGGAGAGGGGCA 59.8 0.09
mm4__chr6 159.98 Mbp_chr6_159980553_R GTCTCGTGGGCTCGGGTGGACAGAGGAGAAAAGCC 58.1 0.09
mm4__chr11 121.80 Mbp_chr11_121795414_F TCGTCGGCAGCGTCCCTTAGCAAAGCTGGCAACC 59.7 0.09
mm4__chr11 121.80 Mbp_chr11_121795414_R GTCTCGTGGGCTCGGGCTCCCCTTCCTTATTTGAGATCT 59.8 0.09
mm3__chrX 106.57 Mbp_chrX_106567124_F Primer3: not found at this Tm N.d. 0.09
mm3__chrX 106.57 Mbp_chrX_106567124_R Primer3: not found at this Tm N.d. 0.09
mm4__chr19 14.12 Mbp_chr19_14117355_F TCGTCGGCAGCGTCCACGGTCTTTGCAGCATGG 59.7 0.09
mm4__chr19 14.12 Mbp_chr19_14117355_R GTCTCGTGGGCTCGGTCATCCTCTCTGCCTACCCC 60.1 0.09
mm4__chr19 1.84 Mbp_chr19_1837332_F Primer3: not found at this Tm N.d. 0.09
mm4__chr19 1.84 Mbp_chr19_1837332_R Primer3: not found at this Tm N.d. 0.09
mm4__chr17 1.64 Mbp_chr17_1643681_F Primer3: not found at this Tm N.d. 0.08
mm4__chr17 1.64 Mbp_chr17_1643681_R Primer3: not found at this Tm N.d. 0.08
mm4__chr3 169.77 Mbp_chr3_169769461_F TCGTCGGCAGCGTCCGGGTAGATAAACTGGGGCTC 59.9 0.07
mm4__chr3 169.77 Mbp_chr3_169769461_R GTCTCGTGGGCTCGGTACCGGTGGTGATCGACAAC 59.7 0.07
mm4__chr18 12.05 Mbp_chr18_12048856_F TCGTCGGCAGCGTCCCTGGCATGGCAGTAAGAGT 59.7 0.07
mm4__chr18 12.05 Mbp_chr18_12048856_R GTCTCGTGGGCTCGGCCTGAGTCCCATCAAGTCCC 59.4 0.07
mm3__chr19 28.86 Mbp_chr19_28863411_F Primer3: not found at this Tm N.d. 0.07
mm3__chr19 28.86 Mbp_chr19_28863411_R Primer3: not found at this Tm N.d. 0.07
mm4__chr10 123.31 Mbp_chr10_123309317_F TCGTCGGCAGCGTCTGGCTTACCTGCTTGTAGCC 60.0 0.07
mm4__chr10 123.31 Mbp_chr10_123309317_R GTCTCGTGGGCTCGGCAACTCTCTGACACTGGGCA 59.6 0.07
mm4__chr2 134.80 Mbp_chr2_134796816_F TCGTCGGCAGCGTCGGCACCAGGCTAGAGCTAAA 59.4 0.07
mm4__chr2 134.80 Mbp_chr2_134796816_R GTCTCGTGGGCTCGGGCCTCCTCTCTCTCCTTTCC 58.8 0.07
mm4__chr10 21.77 Mbp_chr10_21766070_F TCGTCGGCAGCGTCGTTCCCCGTCCCCACAAATT 60.5 0.07
mm4__chr10 21.77 Mbp_chr10_21766070_R GTCTCGTGGGCTCGGAAGGGTTGAAATACGGGCAC 58.4 0.07
mm4__chr16 81.73 Mbp_chr16_81730600_F TCGTCGGCAGCGTCGTCATGGAGACCCGGCTTC 60.1 0.06
mm4__chr16 81.73 Mbp_chr16_81730600_R GTCTCGTGGGCTCGGTGGCAGGGTTGTGCTCTTAG 59.9 0.06
mm3__chr17 19.34 Mbp_chr17_19341717_F Primer3: not found at this Tm N.d. 0.06
mm3__chr17 19.34 Mbp_chr17_19341717_R Primer3: not found at this Tm N.d. 0.06
mm3__chr22 19.96 Mbp_chr22_19962514_F Primer3: not found at this Tm N.d. 0.06
mm3__chr22 19.96 Mbp_chr22_19962514_R Primer3: not found at this Tm N.d. 0.06
mm4__chr6 35.28 Mbp_chr6_35278816_F TCGTCGGCAGCGTCGCAGGCTTCACAAGTGTTGG 59.9 0.06
mm4__chr6 35.28 Mbp_chr6_35278816_R GTCTCGTGGGCTCGGACATCCTGTGAGTGACTCCCT 60.2 0.06
mm4__chr12 32.53 Mbp_chr12_32525210_F Primer3: not found at this Tm N.d. 0.06
mm4__chr12 32.53 Mbp_chr12_32525210_R Primer3: not found at this Tm N.d. 0.06
mm4__chr1 29.90 Mbp_chr1_29898025_F TCGTCGGCAGCGTCCCCAGTAACCCATCTGTGTGG 60.3 0.06
mm4__chr1 29.90 Mbp_chr1_29898025_R GTCTCGTGGGCTCGGACACAAAATGGCGAGTGGTC 59.0 0.06
mm4__chr17 80.92 Mbp_chr17_80922216_F TCGTCGGCAGCGTCGATGAAGCACTCCCTGACCC 60.1 0.05
mm4__chr17 80.92 Mbp_chr17_80922216_R GTCTCGTGGGCTCGGATTCTCCCCAACACCTCAGC 59.6 0.05
mm4__chr9 93.27 Mbp_chr9_93270254_F Primer3: not found at this Tm N.d. 0.05
mm4__chr9 93.27 Mbp_chr9_93270254_R Primer3: not found at this Tm N.d. 0.05
mm4__chr17 81.68 Mbp_chr17_81675712_F Primer3: not found at this Tm N.d. 0.05
mm4__chr17 81.68 Mbp_chr17_81675712_R Primer3: not found at this Tm N.d. 0.05
mm4__chr14 99.07 Mbp_chr14_99065379_F Primer3: not found at this Tm N.d. 0.05
mm4__chr14 99.07 Mbp_chr14_99065379_R Primer3: not found at this Tm N.d. 0.05
mm4__chr12 124.99 Mbp_chr12_124993706_F Primer3: not found at this Tm N.d. 0.05
mm4__chr12 124.99 Mbp_chr12_124993706_R Primer3: not found at this Tm N.d. 0.05
mm4__chr15 35.16 Mbp_chr15_35162181_F Primer3: not found at this Tm N.d. 0.05
mm4__chr15 35.16 Mbp_chr15_35162181_R Primer3: not found at this Tm N.d. 0.05
mm1__chr9 6.83 Mbp_chr9_6834491_F TCGTCGGCAGCGTCCCGCTACCAGCTCACCATAG 59.9 0.05
mm1__chr9 6.83 Mbp_chr9_6834491_R GTCTCGTGGGCTCGGACACGATGAAGGGGAAGACG 59.7 0.05
mm4__chr12 111.06 Mbp_chr12_111062872_F Primer3: not found at this Tm N.d. 0.05
mm4__chr12 111.06 Mbp_chr12_111062872_R Primer3: not found at this Tm N.d. 0.05
mm4__chr12 57.18 Mbp_chr12_57181233_F TCGTCGGCAGCGTCTGTCAACAACGGTGACTGCT 60.1 0.05
mm4__chr12 57.18 Mbp_chr12_57181233_R GTCTCGTGGGCTCGGACAAGGCTGGGAGGAAAGC 59.9 0.05
mm4__chr16 86.55 Mbp_chr16_86554906_F TCGTCGGCAGCGTCTGTAGGTCAAACCGGCTTCC 59.9 0.05
mm4__chr16 86.55 Mbp_chr16_86554906_R GTCTCGTGGGCTCGGATAACGAGAAGACGAGGCGG 59.6 0.05
mm4__chr17 33.71 Mbp_chr17_33708840_F Primer3: not found at this Tm N.d. 0.04
mm4__chr17 33.71 Mbp_chr17_33708840_R Primer3: not found at this Tm N.d. 0.04
mm4__chr19 45.35 Mbp_chr19_45345558_F TCGTCGGCAGCGTCGAGCAGCAATGTCTGTGCAG 59.8 0.04
mm4__chr19 45.35 Mbp_chr19_45345558_R GTCTCGTGGGCTCGGCAGCCCCTGGACCACTTG 59.9 0.04
mm4__chr2 198.05 Mbp_chr2_198053382_F Primer3: not found at this Tm N.d. 0.04
mm4__chr2 198.05 Mbp_chr2_198053382_R Primer3: not found at this Tm N.d. 0.04
mm4__chr7 143.88 Mbp_chr7_143883890_F Primer3: not found at this Tm N.d. 0.03
mm4__chr7 143.88 Mbp_chr7_143883890_R Primer3: not found at this Tm N.d. 0.03
mm4__chr10 48.68 Mbp_chr10_48682563_F Primer3: not found at this Tm N.d. 0.03
mm4__chr10 48.68 Mbp_chr10_48682563_R Primer3: not found at this Tm N.d. 0.03
mm4__chr4 7.74 Mbp_chr4_7739493_F Primer3: not found at this Tm N.d. 0.03
mm4__chr4 7.74 Mbp_chr4_7739493_R Primer3: not found at this Tm N.d. 0.03
mm4__chr2 183.46 Mbp_chr2_183458664_F TCGTCGGCAGCGTCATGTGTGTGGGTAGATGCGG 60.1 0.03
mm4__chr2 183.46 Mbp_chr2_183458664_R GTCTCGTGGGCTCGGTTCCTCCCACCCAGCTTTTC 59.8 0.03
mm4__chr10 131.65 Mbp_chr10_131654985_F Primer3: not found at this Tm N.d. 0.03
mm4__chr10 131.65 Mbp_chr10_131654985_R Primer3: not found at this Tm N.d. 0.03
mm4__chr19 11.51 Mbp_chr19_11506391_F TCGTCGGCAGCGTCATCCACTTAGCCATCGTCGC 60.2 0.03
mm4__chr19 11.51 Mbp_chr19_11506391_R GTCTCGTGGGCTCGGGGTGTAGGCATGGGTTAGCC 60.4 0.03
mm4__chr2 130.85 Mbp_chr2_130847128_F TCGTCGGCAGCGTCCTGTGTGGATTCCTGACGCA 60.3 0.03
mm4__chr2 130.85 Mbp_chr2_130847128_R GTCTCGTGGGCTCGGGAAGGGTAGCCTGCTTCTGG 60.1 0.03
mm4__chr4 8.50 Mbp_chr4_8501198_F TCGTCGGCAGCGTCGGTCGTGCCACTGCTCAG 60.7 0.03
mm4__chr4 8.50 Mbp_chr4_8501198_R GTCTCGTGGGCTCGGCTGCCAGCTCCACCACAG 60.3 0.03
mm4__chr1 111.86 Mbp_chr1_111856568_F TCGTCGGCAGCGTCCTGTCTCTCTCAGCCTCCCT 60.0 0.03
mm4__chr1 111.86 Mbp_chr1_111856568_R GTCTCGTGGGCTCGGTAGCCAGCAGTCACCTTTGG 59.9 0.03
mm4__chr3 82.74 Mbp_chr3_82743244_F Primer3: not found at this Tm N.d. 0.03
mm4__chr3 82.74 Mbp_chr3_82743244_R Primer3: not found at this Tm N.d. 0.03
mm4__chr11 123.43 Mbp_chr11_123430828_F TCGTCGGCAGCGTCCAACATGATGGAGAACCGGC 59.2 0.03
mm4__chr11 123.43 Mbp_chr11_123430828_R GTCTCGTGGGCTCGGGTCGACGAACTGGACCAGAC 60.3 0.03
mm4__chr17 16.48 Mbp_chr17_16481570_F TCGTCGGCAGCGTCTGAGTCCACTGTAGGCCCTT 60.1 0.03
mm4__chr17 16.48 Mbp_chr17_16481570_R GTCTCGTGGGCTCGGCAGACACCTTCTCCTGCCAG 60.0 0.03
mm3__chr16 49.42 Mbp_chr16_49418519_F TCGTCGGCAGCGTCGAACAGCGACTCTCACTGCT 60.0 0.03
mm3__chr16 49.42 Mbp_chr16_49418519_R GTCTCGTGGGCTCGGGTTTTGGGGTTGTGTGAGCC 59.8 0.03
mm4__chr7 149.44 Mbp_chr7_149436660_F TCGTCGGCAGCGTCGCATCTGCTTCAGTTCGCTG 59.9 0.02
mm4__chr7 149.44 Mbp_chr7_149436660_R GTCTCGTGGGCTCGGGCTTCCAGGACTCAGAGCTG 60.1 0.02
mm3__chr11 82.60 Mbp_chr11_82602646_F TCGTCGGCAGCGTCAGACTCAGCATTGACAGCCA 59.3 0.02
mm3__chr11 82.60 Mbp_chr11_82602646_R GTCTCGTGGGCTCGGGTGCTTGTTTCTTAAGAGGAGCT 58.9 0.02
mm4__chr19 5.15 Mbp_chr19_5151470_F TCGTCGGCAGCGTCCTACGTGGCCTTCGTGGAG 60.1 0.02
mm4__chr19 5.15 Mbp_chr19_5151470_R GTCTCGTGGGCTCGGAATTCACAGCAAGCGAACGC 60.3 0.02
mm4__chr5 61.63 Mbp_chr5_61626510_F TCGTCGGCAGCGTCGATGCAAATGGCTTCCCAGC 60.1 0.02
mm4__chr5 61.63 Mbp_chr5_61626510_R GTCTCGTGGGCTCGGGACATTGCCTCAAGTTGCCC 59.7 0.02
mm4__chr17 8.15 Mbp_chr17_8150585_F TCGTCGGCAGCGTCCCGTTGGACTCATTGCCACT 60.6 0.02
mm4__chr17 8.15 Mbp_chr17_8150585_R GTCTCGTGGGCTCGGCCAGGCCTGGGGAATCATTT 60.0 0.02
mm3__chr20 21.04 Mbp_chr20_21044453_F Primer3: not found at this Tm N.d. 0.02
mm3__chr20 21.04 Mbp_chr20_21044453_R Primer3: not found at this Tm N.d. 0.02
mm4__chr6 169.65 Mbp_chr6_169648284_F TCGTCGGCAGCGTCTCTACAGATGCCTTCCGTGC 59.8 0.02
mm4__chr6 169.65 Mbp_chr6_169648284_R GTCTCGTGGGCTCGGCTGGCTCTTCTGCATTTGGC 59.8 0.02
mm4__chr12 115.45 Mbp_chr12_115451876_F Primer3: not found at this Tm N.d. 0.02
mm4__chr12 115.45 Mbp_chr12_115451876_R Primer3: not found at this Tm N.d. 0.02
mm4__chr17 75.96 Mbp_chr17_75963165_F Primer3: not found at this Tm N.d. 0.02
mm4__chr17 75.96 Mbp_chr17_75963165_R Primer3: not found at this Tm N.d. 0.02
mm4__chr6 168.90 Mbp_chr6_168895719_F TCGTCGGCAGCGTCCAGGCTGGCCTTTGTCTAGG 60.3 0.02
mm4__chr6 168.90 Mbp_chr6_168895719_R GTCTCGTGGGCTCGGGGTGATGGTGGTGACCTCAC 60.3 0.02
mm4__chr7 40.62 Mbp_chr7_40616293_F Primer3: not found at this Tm N.d. 0.01
mm4__chr7 40.62 Mbp_chr7_40616293_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 942.30 Kbp_chr1_942302_F TCGTCGGCAGCGTCCAGACCCCAGGTGAGGAGG 60.9 0.01
mm4__chr1 942.30 Kbp_chr1_942302_R GTCTCGTGGGCTCGGCCCTTTGCAATCCCCTACCC 60.3 0.01
mm4__chr14 44.80 Mbp_chr14_44798804_F Primer3: not found at this Tm N.d. 0.01
mm4__chr14 44.80 Mbp_chr14_44798804_R Primer3: not found at this Tm N.d. 0.01
mm4__chr10 29.08 Mbp_chr10_29080312_F TCGTCGGCAGCGTCGTGAAGCAGAGAGGAGCCTG 60.1 0.01
mm4__chr10 29.08 Mbp_chr10_29080312_R GTCTCGTGGGCTCGGGCTGCAGCTTTGCTTCACAT 60.0 0.01
mm4__chr16 84.30 Mbp_chr16_84303327_F TCGTCGGCAGCGTCTATTGGTCTGGCCGCTAAGC 60.1 0.01
mm4__chr16 84.30 Mbp_chr16_84303327_R GTCTCGTGGGCTCGGAAAGCTACCCACATGCCTCC 60.0 0.01
mm4__chr11 68.64 Mbp_chr11_68641801_F Primer3: not found at this Tm N.d. 0.01
mm4__chr11 68.64 Mbp_chr11_68641801_R Primer3: not found at this Tm N.d. 0.01
mm4__chr11 84.72 Mbp_chr11_84720509_F TCGTCGGCAGCGTCCATGGAGCGACAGCCTAGAC 60.2 0.01
mm4__chr11 84.72 Mbp_chr11_84720509_R GTCTCGTGGGCTCGGGAGAGGCTCGGTACTTGCC 59.8 0.01
mm4__chr2 239.14 Mbp_chr2_239144382_F Primer3: not found at this Tm N.d. 0.01
mm4__chr2 239.14 Mbp_chr2_239144382_R Primer3: not found at this Tm N.d. 0.01
mm4__chr17 21.13 Mbp_chr17_21127185_F Primer3: not found at this Tm N.d. 0.01
mm4__chr17 21.13 Mbp_chr17_21127185_R Primer3: not found at this Tm N.d. 0.01
mm4__chr11 70.82 Mbp_chr11_70820987_F TCGTCGGCAGCGTCGTGGGGTCTGAGCTTCCATC 60.1 0.01
mm4__chr11 70.82 Mbp_chr11_70820987_R GTCTCGTGGGCTCGGTCAGTGCCCTCTCTGCTTTG 59.9 0.01
mm4__chr3 195.88 Mbp_chr3_195875665_F TCGTCGGCAGCGTCCAGCCCTTCCTTCCAGGC 60.0 0.01
mm4__chr3 195.88 Mbp_chr3_195875665_R GTCTCGTGGGCTCGGCAGGAAGTCCTCATCCGCC 59.8 0.01
mm4__chr1 149.01 Mbp_chr1_149008369_F TCGTCGGCAGCGTCGACACAAGCTGAGGTATCCCA 59.4 0.01
mm4__chr1 149.01 Mbp_chr1_149008369_R GTCTCGTGGGCTCGGAGAAATTAGGCTGGTCCCACT 58.7 0.01
mm4__chr1 143.54 Mbp_chr1_143542937_F TCGTCGGCAGCGTCGACACAAGCTGAGGTCTCCC 60.0 0.01
mm4__chr1 143.54 Mbp_chr1_143542937_R GTCTCGTGGGCTCGGAGAAATTAGGCTGGTCCCACT 58.7 0.01
mm4__chr1 120.51 Mbp_chr1_120513868_F TCGTCGGCAGCGTCAGAAATTAGGCTGGTCCCACT 58.7 0.01
mm4__chr1 120.51 Mbp_chr1_120513868_R GTCTCGTGGGCTCGGGACACAAGCTGAGGTATCCCA 59.4 0.01
mm4__chr14 72.64 Mbp_chr14_72642726_F TCGTCGGCAGCGTCCATGCCCCTGTCTCACTCTC 59.8 0.00
mm4__chr14 72.64 Mbp_chr14_72642726_R GTCTCGTGGGCTCGGACACTGGGGTTGTGTTCTGG 60.1 0.00
mm4__chr17 21.41 Mbp_chr17_21405808_F Primer3: not found at this Tm N.d. 0.00
mm4__chr17 21.41 Mbp_chr17_21405808_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 21.90 Mbp_chr1_21896695_F TCGTCGGCAGCGTCGGACACCCAGGACATGAGTG 60.0 0.00
mm4__chr1 21.90 Mbp_chr1_21896695_R GTCTCGTGGGCTCGGTTGACTTCTGCCTGTGTCCC 59.8 0.00
mm4__chr20 62.49 Mbp_chr20_62493808_F TCGTCGGCAGCGTCGTGAGGGTGGACCAGATTCC 59.7 0.00
mm4__chr20 62.49 Mbp_chr20_62493808_R GTCTCGTGGGCTCGGAGGCACTCAGTCCATGCATG 60.3 0.00
mm4__chr17 5.90 Mbp_chr17_5899311_F Primer3: not found at this Tm N.d. 0.00
mm4__chr17 5.90 Mbp_chr17_5899311_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 238.92 Mbp_chr2_238918463_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 238.92 Mbp_chr2_238918463_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 151.01 Mbp_chr6_151007648_F TCGTCGGCAGCGTCTGTGCTAACCTGCAGGACAG 59.9 0.00
mm4__chr6 151.01 Mbp_chr6_151007648_R GTCTCGTGGGCTCGGTGGCATCATGGACCCCAAAA 59.8 0.00
mm4__chr2 122.70 Mbp_chr2_122704344_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 122.70 Mbp_chr2_122704344_R Primer3: not found at this Tm N.d. 0.00
mm4__chr14 65.30 Mbp_chr14_65304549_F Primer3: not found at this Tm N.d. 0.00
mm4__chr14 65.30 Mbp_chr14_65304549_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 177.49 Mbp_chr5_177489734_F TCGTCGGCAGCGTCGTGACTCTAAAGGGGTGCCC 60.0 0.00
mm4__chr5 177.49 Mbp_chr5_177489734_R GTCTCGTGGGCTCGGTGGCCTGGTGAGAGGGAG 60.2 0.00
mm4__chr7 63.11 Mbp_chr7_63113867_F TCGTCGGCAGCGTCGGAGGAGGACTTGGACAGGG 61.2 0.00
mm4__chr7 63.11 Mbp_chr7_63113867_R GTCTCGTGGGCTCGGGCTACTGCCCAAGGTACAGG 60.1 0.00
mm4__chr7 36.29 Mbp_chr7_36286588_F TCGTCGGCAGCGTCCTGTGTTTCTGCACTTGCCC 59.9 0.00
mm4__chr7 36.29 Mbp_chr7_36286588_R GTCTCGTGGGCTCGGTCTGGAGCCCACTATAGCCA 59.7 0.00
mm4__chr7 149.73 Mbp_chr7_149725176_F TCGTCGGCAGCGTCCTCCCCTCCTGTCTTCATGC 59.8 0.00
mm4__chr7 149.73 Mbp_chr7_149725176_R GTCTCGTGGGCTCGGACGTGCTTTCTACAGGGGTG 59.9 0.00
mm4__chr7 128.87 Mbp_chr7_128868092_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 128.87 Mbp_chr7_128868092_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 109.44 Mbp_chr6_109443795_F TCGTCGGCAGCGTCCTGTGGGCAGGTGCATTCTA 60.0 0.00
mm4__chr6 109.44 Mbp_chr6_109443795_R GTCTCGTGGGCTCGGAGGAGTAGGGCTGGCTGAG 60.3 0.00
mm4__chr5 28.90 Mbp_chr5_28895707_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 28.90 Mbp_chr5_28895707_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 141.95 Mbp_chr5_141945838_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 141.95 Mbp_chr5_141945838_R Primer3: not found at this Tm N.d. 0.00
mm4__chr20 46.17 Mbp_chr20_46174449_F TCGTCGGCAGCGTCGGGGAAACGCGTTGTCCT 60.2 0.00
mm4__chr20 46.17 Mbp_chr20_46174449_R GTCTCGTGGGCTCGGCTCCTAGCCCCTCGCCCT 62.5 0.00
mm4__chr2 86.26 Mbp_chr2_86255089_F TCGTCGGCAGCGTCAGCAAACCAGGAACACAGGT 59.7 0.00
mm4__chr2 86.26 Mbp_chr2_86255089_R GTCTCGTGGGCTCGGTAAATGAGCACAGCCCAGCA 59.9 0.00
mm4__chr2 42.37 Mbp_chr2_42369066_F TCGTCGGCAGCGTCTTTACCATTCTGGGCGGGTC 60.0 0.00
mm4__chr2 42.37 Mbp_chr2_42369066_R GTCTCGTGGGCTCGGCATCACGGTGATAGGCCACA 59.8 0.00
mm4__chr2 25.19 Mbp_chr2_25190657_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 25.19 Mbp_chr2_25190657_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 215.25 Mbp_chr2_215246145_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 215.25 Mbp_chr2_215246145_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 18.85 Mbp_chr19_18851906_F TCGTCGGCAGCGTCCAGGTTCACCCAAGTACCGG 60.3 0.00
mm4__chr19 18.85 Mbp_chr19_18851906_R GTCTCGTGGGCTCGGCTGGGAACGGGAACTCCATG 60.3 0.00
mm4__chr16 57.91 Mbp_chr16_57911224_F TCGTCGGCAGCGTCACAGCGTATTTGCACACCCT 60.2 0.00
mm4__chr16 57.91 Mbp_chr16_57911224_R GTCTCGTGGGCTCGGGCCAGGTCACTACATCTTCCC 60.1 0.00
mm4__chr16 57.69 Mbp_chr16_57689401_F Primer3: not found at this Tm N.d. 0.00
mm4__chr16 57.69 Mbp_chr16_57689401_R Primer3: not found at this Tm N.d. 0.00
mm4__chr16 55.33 Mbp_chr16_55327338_F TCGTCGGCAGCGTCGGAGGCTGCAGGGAGTTTTA 59.6 0.00
mm4__chr16 55.33 Mbp_chr16_55327338_R GTCTCGTGGGCTCGGTCACAGTGCCAGTCACCAAG 60.1 0.00
mm4__chr16 51.26 Mbp_chr16_51255816_F TCGTCGGCAGCGTCAACAGCAGACTCCTGGAAGG 59.3 0.00
mm4__chr16 51.26 Mbp_chr16_51255816_R GTCTCGTGGGCTCGGAGCAACTTGGAAATGGCTGA 58.0 0.00
mm4__chr16 12.67 Mbp_chr16_12674312_F TCGTCGGCAGCGTCCCTCCTGGGTTCTGATGCTG 60.1 0.00
mm4__chr16 12.67 Mbp_chr16_12674312_R GTCTCGTGGGCTCGGCTGGCTCCAACCCAAGAAGT 59.8 0.00
mm4__chr16 1.20 Mbp_chr16_1202215_F TCGTCGGCAGCGTCCATGCCGACTGCCACATAGA 60.1 0.00
mm4__chr16 1.20 Mbp_chr16_1202215_R GTCTCGTGGGCTCGGGCAGGATCGTGGGGTAGTTC 60.1 0.00
mm4__chr12 53.72 Mbp_chr12_53722053_F TCGTCGGCAGCGTCCTGTTCCATCAGCTCCGTGT 60.0 0.00
mm4__chr12 53.72 Mbp_chr12_53722053_R GTCTCGTGGGCTCGGAGCCAGCAAGAACGGAATGA 59.9 0.00
mm4__chr10 62.40 Mbp_chr10_62397056_F TCGTCGGCAGCGTCGTTGATGCCAGTGCCTTTGG 60.0 0.00
mm4__chr10 62.40 Mbp_chr10_62397056_R GTCTCGTGGGCTCGGAGGCACCGTTATCACATGAGG 60.1 0.00
mm4__chr10 132.54 Mbp_chr10_132537008_F TCGTCGGCAGCGTCCTGCGAGGGTTCCCTGCA 62.0 0.00
mm4__chr10 132.54 Mbp_chr10_132537008_R GTCTCGTGGGCTCGGCAGAGAAGACGGAGGGGAGG 61.0 0.00
mm4__chr1 46.51 Mbp_chr1_46508158_F TCGTCGGCAGCGTCTGAGCAGCTGGTAAACCCAC 60.2 0.00
mm4__chr1 46.51 Mbp_chr1_46508158_R GTCTCGTGGGCTCGGGATACCCTCCTGTTGTGCCC 60.1 0.00
mm4__chr1 39.97 Mbp_chr1_39970533_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 39.97 Mbp_chr1_39970533_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 228.22 Mbp_chr1_228219110_F TCGTCGGCAGCGTCGTCTGCGAAGGACACTCAGG 60.3 0.00
mm4__chr1 228.22 Mbp_chr1_228219110_R GTCTCGTGGGCTCGGCGTAGCTCTTCCCATGCACA 60.1 0.00
mm3__chr2 118.18 Mbp_chr2_118179648_F Primer3: not found at this Tm N.d. 0.00
mm3__chr2 118.18 Mbp_chr2_118179648_R Primer3: not found at this Tm N.d. 0.00
mm3__chr19 48.49 Mbp_chr19_48490780_F TCGTCGGCAGCGTCTCTCTCTCCTTGTCTGCCCC 60.6 0.00
mm3__chr19 48.49 Mbp_chr19_48490780_R GTCTCGTGGGCTCGGCCCGACTCTTGCCCATCTTT 60.0 0.00
mm3__chr10 47.97 Mbp_chr10_47971772_F TCGTCGGCAGCGTCGAATGGTGTGGAAAGGCCCT 60.2 0.00
mm3__chr10 47.97 Mbp_chr10_47971772_R GTCTCGTGGGCTCGGGCCTCTCTGTGACCTTGGAC 60.0 0.00
mm3__chr10 46.41 Mbp_chr10_46412860_F TCGTCGGCAGCGTCGCCTCTCTGTGACCTTGGAC 60.0 0.00
mm3__chr10 46.41 Mbp_chr10_46412860_R GTCTCGTGGGCTCGGGAATGGTGTGGAAAGGCCCT 60.2 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529598 CACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGAGCCGTTGTCGACGA
CGAGCGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC
mm1__chr9 100.73 Mbp_chr9_100731275 GCCAGCTCACCATGGATGATGATATCGCCCCACTTGTCATTGACAACGGCTCAGGCATGTGCAAGGCCGGCTTTGCAGGT
GATAATGTCCCCCTGGGCCATCTTCCCCCTCCATCGTGGAGCTCCCTAG
mm1__chr5 98.34 Mbp_chr5_98338779 AGCACGCTCCTCATCAACAAGGGCTCTGGCATGTGCAAGGCCGGCTTCACAGGTGACAATGCCCCCGGGCCATCTTCCCC
TCTGTCATGGGTGCCAGGGAGTGATGGTGGGCATGGGTCAAAA
mm1__chr22 15.72 Mbp_chr22_15721522 CCGTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCT
TCCATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm1__chr2 131.63 Mbp_chr2_131626174 ATACCGCTGTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCAGGCTGTC
TTCCCTTCTATCGTGGGGCGCCCCAGGCACCAGGGCATGATGGAGGGCATGCATCAGAAG
mm1__chr2 131.26 Mbp_chr2_131263591 TGGATGATGATACCGCCGTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCC
CGGGCTGTCTTCCCTTCCATC
mm1__chr2 130.66 Mbp_chr2_130656785 ATACCGCTGTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTC
TTCCCTTCCATC
mm1__chr2 130.46 Mbp_chr2_130463884 GATGGAAGGGAAGACAGCCCGGGGGGCATCGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGA
GCACGG
mm1__chr2 130.08 Mbp_chr2_130075312 GATGGAAGGGAAGACAGCCCGGGGGGCATCGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGA
GCACAGCGGTAT
mm1__chr14 19.40 Mbp_chr14_19402849 ATCATGTTCTGGTGCCTGGGGCACCCCACGATGGAAGGGAAGACAGCCCGGGGGGCATCGTCACCTGCAAAGCCGGCCTT
GCACATGCCAGAGCCGTTGTCAATGACGAGCACGG
mm1__chr14 19.00 Mbp_chr14_18998903 CCGTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCT
TCCATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm1__chr1 223.86 Mbp_chr1_223864792 CATGGAAGGGAAGATGGCCCAGGGGTCATCATCCCCTGTGAAGCCAGCCTTGCACATGCCGGAGCTGTTGTCAATGACGA
GCGCCGCGATATCATCATCCATGGTGAGCTGGC
mm2__chr22 41.08 Mbp_chr22_41075180 ATGCCCACCATCATGACCTGGTGCCTGGTGCACCCCACGATGGAAGGGAAGATGGCCCAGGGGACATCGTCACCCGCGAA
GCCGGCCTTGCACATGCCAGAGCTGTTGTCAACAACGAGCGCAGCAATA
mm3__chr11 44.89 Mbp_chr11_44891604 AAACCTTCAGGGAGGCAACCTGGAGGATAAGAAACAAGACTCAGTCCAGGTGAGCCAGAGCAGGTGTTGCCAGAAGCTCT
GGCTTGTGCAAGGCTGGGCAGCGGACACGGTGAGGATTGGTGGGACTG
mm2__chr5 61.83 Mbp_chr5_61832986 CCATCATCATGCCCTGGTGTCAGGGGTGCCCAACGATGGAGGGGGAACATGGGTTGGGGGACGTTGTGTCCAGCAAAGCC
AGCTTTGCACATGCTGGAGCCATTGTCAATGACCAGCCCATGA
mm2__chr20 1.16 Mbp_chr20_1160619 GCTCCTCCACCGATCACAATGGAAGAAGAGATCACCATACTCGTCATTGACAATGGCTCCAGCATGTGCAAAGCTGGTTT
TGCTGGGGACGACGCCCCTGGAACCATGTTTCCC
mm2__chr1 237.93 Mbp_chr1_237926866 CTCCTCCACCAGTCACGATGGAAGAAGAGATCGCCACGCTTGCCATTGACAATGGCTCCAGCATGTGCAAAGCTGGCTTT
GCTGGGGACGATGCCCCCCAAGCTGTGTTTCCCTCCA
mm1__chr17 81.51 Mbp_chr17_81512295 CCATCCACTCACCTGGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCGTCCCCAGCAAAACCAG
CTTTGCACATGCCGGAGCCATTGTCAATGACCAGCGCGGCGATCTCTTCTTCCAT
mm2__chrX 53.14 Mbp_chrX_53143854 GATGGAGGGGAACACAGCTCAGGGGCCATCCATCCCCAGCAAAGCCAGCTTTGCACATGCCAGAGCCATTGTCAATGATG
AGCGCGGCGATCTCTATTTCCATTGCAACCTGTGGAAGAGCA
mm2__chr2 57.76 Mbp_chr2_57764988 AGATTGCTGGCTCGTCAATGATAGTGGCTCTGGCATGTGCAAAGCTGGCTTTGCTGGGGAAGACACCCCCTGAGCCATGT
TTCTCTCCATCGTCAGGTGCCCCTGGCACCACAGTGTGATAGTGGGCATGGGCAAGAAG
mm3__chr3 139.49 Mbp_chr3_139493850 TGCCGGTCACAATGGAAGAAGAAATCACCATGCTTTTCATTGACATTGGCTCCAGCATGTACAAAGCTGGCTTTGCTGGG
GTGATGCTTCCCGAGCCATGTTCCCTTCCAT
mm4__chr4 79.53 Mbp_chr4_79525832 GCATCAGGCACAAGAAAGAGTCCAAGAATGCTCAGGCATGTGCAAAACTGGAGAAGTAACACAGAAGCAAGGAGAGATCA
TGCTGAATAGGCATTGACAAAAATGGTTATATGTGAAAGGACCTCGACAAGACCAGT
mm4__chr1 202.87 Mbp_chr1_202873329 CCTCGGCTGATCACAATGGAAGAAGAGATCATGGCACTTGTCATTGACAATGGCTCCAGCACATGCAAAGCTGGCTTTGC
TGGGTATAGTTCCCCGAGCCATGTTCCCCTCCATC
mm3__chrY 17.76 Mbp_chrY_17758066 AACGGTGGAGGGAAACATGGCTTGGGGAGCATCGTCCCCTGCAAAGCCAGCTTTGCACATGCAAGAGCCATTGTCAATGA
CGAGCGCAGCGATCTCTTCTTCTACTGCGATGGACAGAGGAGTGGGACAGAGGA
mm4__chr8 42.42 Mbp_chr8_42416144 CACCACCGCTACAGAGGATTAAGGGGTTTCCCTCTGGGGGCTCCAGAATATGCAAGCCAGGGTGTTTTTGGCTGCTTGAG
CAAAGGATAAGACCTGCACTTCCCTGC
mm3__chr3 12.07 Mbp_chr3_12070260 GCCATGCTGGTCATTGACAGTGTCTCCGGCATGTACAAAGCTGGATTTGCTGGGGACGACGCCCCCTGAGCCGTGTTCCC
CTCCATCCTTAGGCGCCCCTGGCACCAGGGCACGATGGTGGGCATGGGTCAGAAGGACTC
mm2__chr2 73.90 Mbp_chr2_73901350 GTGTGAAGAGGAGACCACCGCGCTCGTGTGTGACAATGGCTCTGGCCTGTGCAAGGCAGGCTTCGCAGGAGATGATGCCC
CCCGGGCTGTCTTCCCCTCCATTGTGGG
mm4__chr16 1.14 Mbp_chr16_1144003 GAAGCACCACGTCCAGAGAAAGAGGCCGGCCTTGCACACATCGCAGCCAGGCAGGGAGACAGGAGGCCAAGGCGGTTAGA
CAGCCAGGCGGGGAGACGGGAGGCCGGCCTTGCACACATCACAGCCAG
mm4__chr9 124.32 Mbp_chr9_124315722 GGTCCCTGGTCACACAGATGTGCCACCGCAGCCCCTGCCTTGCAGGAGCCTGAGCCTGGCGTAAACCCACATGGCTGCAG
AGGGGATTTGGTCC
mm3__chr5 57.48 Mbp_chr5_57482645 GTCGAGGACGCCCTATCATGGAGGGGAACACAGCCCGGGGGGCATCGTCACCACCAAAGCCTGCCTTGCACATCCCTGAC
CCATTATCCACTACCAAGGCAGACAGCTCATTGTCAGTCATGGTGCTGGCTGAGATCTTC
mm4__chr10 49.98 Mbp_chr10_49977914 AGTTGTTGGGAGTGGCTGAGGACATTGGGGAGCTTCTGTCTGAGTTGGAAGAGTGGAGTCTCAACAAGAACCATTGTATT
CTGGACATGTGTGGGCCCTGGAGTGTGCAAGGCTGGATGCCTTGTCTACCTGTGCC
mm4__chr10 45.86 Mbp_chr10_45858151 TAAGGCACAGGCAGACAAGGCATCCAGCCTTGCACACTCCAGGGCCCACACATGTCCAGAATACAATGGTTCTTGTTGAG
ACTCCACTCTTCCAACTCAGACAGAAGCTCCCCAATCTCCTCAGCCACTCCCAACAACT
mm4__chr16 1.14 Mbp_chr16_1144163 CTTGCACACATCGCAGCCAGGCGGGGAGATGGGAGGCCAGCCTTGCACACATCGTAGCCAGGCGGGCAGACAGCCAGGCA
GGGAGACGGGCGGCCCCCATTTCCCTGGGGTAGAGCAAGGCTGGTTT
mm4__chr6 14.50 Mbp_chr6_14501462 TCATCCTCTGGCCTTTTCAGGATTTCCATAGCCTAACTAGAAGTATCTCCATGGAAACCAAGCAGTCCTGCTTTGCACAT
CCCGGAACTTTTTGTTCCTGACAAAGCTTGACAAATCACATGCAGCATGGAGAGT
mm4__chr8 143.38 Mbp_chr8_143380411 CACTGGGAGCAGCTCATCCCTGGCCCCTGCTTTGCACGTGGCAGAGCCCTCCTGCACAGCCAGCTCCTCACCCCCGTGGC
GCGCACCCCCAACGAAAGTGGCTGTGATGAGC
mm0__chr5 77.78 Mbp_chr5_77784922 ATATCACCGCGCTCGTCATTGACAACGGCTCCGGCATGTGCAAGGCCAGCTTCACGGGCGACAATGCCGCCCGGGCAGTC
TTCCCCTCCATCGTT
mm3__chr16 50.06 Mbp_chr16_50059172 GAGGGGAACATGGTTCAGGGGGCATCGTCCCTAGCAAAGCCAGCTTTGCACCTGCCAGAGCCATTGTCAATGATGAGCAC
GATGATCTCTTCTTCCATCGCCATCGTGGAGAAGT
mm4__chr7 45.65 Mbp_chr7_45651375 TCTGTTGGGACCATCTTGGCATCTGGTGCATCCCCTGCCAGCCACATTGGGCTGTGTGTGTCCCTGCCTTGCACATGCAG
GATGGAATATGTTTATCAACAGGGGGAGCTTGCCCAGGCAGAGGAGGACACTTT
mm4__chr20 23.03 Mbp_chr20_23034826 CTGAAATTCACCAAGCCGCCAGTGGAAGCCTCAGGTTCCGCCTCTATAAAAGGGACTCAGCCATGTGCAAGGATGGGTCG
TCGCGAGGATTAAAGGGCAGGGTGAGGGGATGTCAAGTGCAAGGCAAGTGG
mm1__chr15 43.99 Mbp_chr15_43990125 AATGAAGGGGAAGATGGCCCGGGGTGGGCATCGTCACCTGCAAAGCTGGCCTTGCACATGCCAGAGCCATTGTCGACGAC
AAGCATGGTGATATCATCATCCATGGTGAGCTGGC
mm4__chr14 61.53 Mbp_chr14_61529129 GAGGGTCACTGTAAACTGGCAGACTTCGGAATGTGCAAGGAGGGGATTTGCAATGGTGTCACCACGGCCACATTCTGTGG
CACGCCAGACTATATCGCT
mm2__chr5 80.30 Mbp_chr5_80302788 ATGATGAAGGGGAAGGCAGCCCGGGGGACACCATCGCTCGCAAAGCTAGCCTTGCATATGCCAGAGCCATTGTTGAATAC
GAGAGCAGTGATATCGTCATGCATGGTGAGCT
mm4__chr2 91.59 Mbp_chr2_91588358 GCAGACACAGTCTCCTTCCGGGAGCAGGCAGGTGCAAGGCAGGCACCGGGGCTTTGGAAGGGGCTGGTGGAGATGCTTGG
GTGCCGGTAGCCCCAGGGCAGACCCTGGGGCCTCCCTCATTCCAGCTCCTCTGC
mm4__chr1_KI270712v1_random 131.34 Kbp_chr1_KI270712v1_random_131344 ACTTAATGGCACCCGAGCAGGGCGCCCTGGCAGACGCAGTCTCCTTCCGGGAGCAGGCAGGTGCAAGGCAGGCACCGGGG
CTTTGGAAGGGGCTGGTGGAGATG
mm4__chr1 144.79 Mbp_chr1_144787693 CATCTCCACCAGCCCCTTCCAAAGCCCCGGTGCCTGCCTTGCACCTGCCTGCTCCCGGAAGGAGACTGCGTCTGCCAGGG
CGCCCTGCTCGGGTGCCATTAAGT
mm4__chr1 144.18 Mbp_chr1_144178671 ACTTAATGGCACCCGAGCAGGGCGCCCTGGCAGACGCAGTCTCCTTCCGGGAGCAGGCAGGTGCAAGGCAGGCACCGGGG
CTTTGGAAGGGGCTGGTGGAGATG
mm4__chr1 143.35 Mbp_chr1_143353086 ACTTAATGGCACCCGAGCAGGGCGCCCTGGCAGACGCAGTCTCCTTCCGGGAGCAGGCAGGTGCAAGGCAGGCACCGGGG
CTTTGGAAGGGGCTGGTGGAGATG
mm4__chr1 194.30 Mbp_chr1_194299168 AGACAGTTGGTGAGCAATAGGAAAATCATATTTCAACCATATGTCCATGATGCACACAGCCTGGCTTGTACATCCCTGAG
CCTGAATCTTGAGCATGGGTCTTCTTTGCTCAGGCGTTGTCACATGGAA
mm4__chr7 80.58 Mbp_chr7_80578924 CCTGGAAGCAACTGAAATTTCAGGCTATCAATAATTGTAGAAAGGATAGAAATGCACAAAGATTGAAGAAAATAGTGAAG
CTCAGGAATGGGCAAGGCTGGTTAAATCTTAATAAACATGACAGTGAGAAGCTTGGACCC
mm3__chr19 30.42 Mbp_chr19_30423754 GTCTGCTCAATGTGTCCCCAGCCGCTGAGGGCCTGCCCAGACTGTGCGAAGAGCATGCAAGGGCAGGGCTCCCTCCCGGG
GGCTTCGGCAGGAGCAAGGCTGGCAGTGACAGCAGCTGTGGC
mm3__chr3 4.49 Mbp_chr3_4492381 CATTTGAGGCAAGACAGGGCAGTCGGCTGACTGCCTCCGGGCTTAGCTCTTCCCCTCCTTGCAAAAGCCGGAGCCCTAAG
CAGCGTGTAGCCCGGGAGCCCAGGCATTACTTGGCAGCGGGTGAA
mm4__chr16 52.74 Mbp_chr16_52741230 AGAATTCCCTCAGAAATCAGACCAAAATTACATTTCAAAATTATTTTTTTCTCCCTAAAATAAAAGTTCACATTGCTTTG
TCTCCTCCTGCATGTCCAAGGCAGGCAATGGCTAAGAATTTATTTTCTCCAGC
mm4__chr2 46.15 Mbp_chr2_46145147 AGAAGGTCACTGCAAGCTGGCTGACTTCGGGATGTGCAAGGAAGGGATTCTGAATGGTGTGACGACCACCACGTTCTGTG
GGACTCCTGAC
mm4__chr20 16.57 Mbp_chr20_16573540 ATCCATGCGCCACGGTTTGGCGGCGGCCGGGCGGTGCAGAGGGGCGGGGCCGCGGGGCCTGGAAGCAGCCTCCGGGATCT
GCAAGTCTGGAAAGGCCACGTTGTGGACCTTGGCGCAGAGGTTAT
mm3__chr11 1.80 Mbp_chr11_1804036 CACGATGGAGGGGAAGACGGCCCAGGGGGCATCGTCGTCGGTGAAGCTGGCCTTGCACATGCCAGAGGTGTTGTCTATGA
CAAGCAAGGTGATATTATCATCCATGGTGAGCTGGG
mm4__chr18 46.53 Mbp_chr18_46528927 ATCCAGCCAACAACCTCGAGCTCCAGCAGGCCTCCTGCTTGTCCAAGGCCGGCCTTCTGCTCCACCTCATCCCCTATGCA
GGATCCAGGGTGGGTGCATCAGCTAGGAG
mm4__chr10 127.32 Mbp_chr10_127318490 TAACCATGTGTGCAGGAGGCAGTAGCCTCCTGGGGAGAGGCAGCAGCATGTGCAAGGATGGGGGCCCAGCACTGGGTTCA
TCCTACAAGCATGTGTCCGGGCCTCAAGGCCACTCTGATGACTGCTT
mm4__chr6 159.44 Mbp_chr6_159443588 GAGACTTCATAGCCACTGGGGTTTGAAGATCTTATCTGATGCTGATGGCTTTTGCCAAAGGGCTCCATCATTTGCAAGGA
GGGGTGGGGATACTTTATATGAGGAATCTGGCTTTGCCGAAGCTTTCCAGGTCTA
mm4__chr7 44.44 Mbp_chr7_44444136 CGAAGGTTTGCTGTATTGAGAACAGAATTTCTGGAATGAGAAGGCTCAGGCCAGTGCAAGGAGGGCTACAGACTCAGGGT
CCCTGCCCGCCACTTTAACCTTCT
mm4__chr18 37.78 Mbp_chr18_37778138 CTGACGTCCACTTTAGCCTGTGCCAGTCTTGCCCATGGCAGAGCCCCTGTTCATGCCCTAAGTTTAGACATTTTCCAGAG
CCAGCCTCAG
mm4__chr21 45.18 Mbp_chr21_45179183 GTCACAGCCTCAGTTCCTCGCGTCCGCTCACCCCTGCATTGCAGATGCCGCTGCCTGCGGGTGCATGGATCCCTGCCACC
AAAG
mm4__chr6 159.98 Mbp_chr6_159980553 TAAGAAGGTGGAGAGGGGCAGCCCCAGCTGGGTGTTAGGCTTTGCGGAGCCTCTGCTGTCTGATGTTTGGCTCCAGCATC
TTCAAAGCAGGGGCTTTTCTCCTCTGTCCAC
mm4__chr11 121.80 Mbp_chr11_121795414 CCTTAGCAAAGCTGGCAACCTCCAGGCCTGCTCCCACAGCCTGCCTTTCACATATCCGAGCCCTCTGTGACACATCAGAT
TTAATGAGACTGCACAGATCTCAAATAAGGAAGGGGAGC
mm4__chr19 14.12 Mbp_chr19_14117355 CACGGTCTTTGCAGCATGGGGCGGCCCTAGGGGAAGGGGACCCCTACAGGGGGTACTCTGCGGGCCCGCGGGCCCCAGCC
TTGGACAGGCCGGGGCAAGGGGCGCTGGGGGGTAGGCAGAGAGGATGA
mm4__chr3 169.77 Mbp_chr3_169769461 CGGGTAGATAAACTGGGGCTCCCGGCACCCAGCCACGCCCGCCTTGATCATTCCCGAGCCGTTGTCGATCACCACCGGTA
mm4__chr18 12.05 Mbp_chr18_12048856 CCTGGCATGGCAGTAAGAGTTCTACTAATGGCCAACTCAGTACTTCTAGCTCTCCCTGCCTCCCTGCCGGACCTGCACAT
GCCTGAGGCCTGCCAGTGACACTGGGACTTGATGGGACTCAGG
mm4__chr10 123.31 Mbp_chr10_123309317 TGGCTTACCTGCTTGTAGCCCTACTTCCCAGATTCTTTCCCTTCATCATCTCATGGTGTCTTCGCCATGTGCAAGGTGGG
TGGAGTGACATTTACTCATTCGTTTGAGGAGACTGAAGCCTGCCCAGTGTCAGAGAGTTG
mm4__chr2 134.80 Mbp_chr2_134796816 GGCACCAGGCTAGAGCTAAATGCCTCAAATCTAGGACCTACAGACAGATACTGGACCTAGGCCTTTGAGACTCCTGCAGT
TGCAAGGCAGGGGAAAGGAGAGAGAGGAGGC
mm4__chr10 21.77 Mbp_chr10_21766070 GTTCCCCGTCCCCACAAATTAAACTATAGAAGACGATCTGTATACTTATTTAACCTTATTAATTAAATACATCTTCTCGG
CACCAGTATGTGCAGGGCTGGACTGGCTTGCTGGTGCCCGTATTTCAACCCTT
mm4__chr16 81.73 Mbp_chr16_81730600 GTCATGGAGACCCGGCTTCCCTGCCCAGCGTTGCAGCTGCGGGAGCCCGGGCTGGGAGGCTCTGTGAGGCTGAGGGAAGC
AGAGGGCACAGACCTGGGTCCTGCAGGCGGTGGCTCCACCTAAGAGCACAACCCTGCCA
mm4__chr6 35.28 Mbp_chr6_35278816 GCAGGCTTCACAAGTGTTGGGGTCCACTGCTAGAGCAGGCTGCACACCACAGTGGGTACAGAAGATATCCTGCCAGCAGG
AAGCTTCAGTGCTTCCCACCTTGCACATGAGGGACCCAGGGAGTCACTCACAGGATGT
mm4__chr1 29.90 Mbp_chr1_29898025 CCCAGTAACCCATCTGTGTGGTTTTTCACTCCTGCCCTGCATCTGCCTGAGCCAAGACCACTCGCCATTTTGTGT
mm4__chr17 80.92 Mbp_chr17_80922216 GATGAAGCACTCCCTGACCCTCCGTGAATGCCACATCGCCCGGCTCCGGAAAGAGCATGGCTGGTGGATTCTTCCCGGGC
CTCAACTCGCAGCCTCTGACCCGAGAGCTGAGGTGTTGGGGAGAAT
mm1__chr9 6.83 Mbp_chr9_6834491 CCGCTACCAGCTCACCATAGATGATGATATTGCTACGCCCGTCGTCGACAACGGCTCCGGCATGTGGAAGGCTGGCTTCG
CAGGTGACGATGACCCCCGGCCGTCTTCCCCTTCATCGTGT
mm4__chr12 57.18 Mbp_chr12_57181233 TGTCAACAACGGTGACTGCTCCCAGCTCTGCCTGCCCACGTCAGAGACGACCCGCTCCTGCATGTGCACAGCCGGCTATA
GCCTCCGGAGTGGCCAGCAGGCCTGCGAGGGTCAGTGCCTGGCTTTCCTCCCAGCCTTGT
mm4__chr16 86.55 Mbp_chr16_86554906 TGTAGGTCAAACCGGCTTCCGATCCTGTTCCTGAGCCTCAGTTTCCCCGACCTCAAGGGGCCCACGGGCTCCCCCACGTG
CACGGCAGGTGTCCCTCCCGCCTCGTCTTCTCGTTAT
mm4__chr19 45.35 Mbp_chr19_45345558 GAGCAGCAATGTCTGTGCAGGTAGCGGCTCCTGGAAGTGCAGGGCTGGGCCCAGAGCGCCTGAGCCCTGAGGAGCTGGTG
CGGCAGACGCGGCAAGTGGTCCAGGGGCTG
mm4__chr2 183.46 Mbp_chr2_183458664 ATGTGTGTGGGTAGATGCGGCATGGACTCTTGCTGGGGCAGCCTAGTCTTGGCTCAGCCCTGTGCAGGGCGGGGGGTAGG
TGTTAGGAAAAGCTGGGTGGGAGGAA
mm4__chr19 11.51 Mbp_chr19_11506391 ATCCACTTAGCCATCGTCGCCTGGTCATGAGCACCCGCCATGCACATGGCGAAGACAGGGCCTTCCTCCACTGTTGGAAG
ACATGCACCCTTAAGGTTTGCACAGTGGGGGCTGCAGCGGCTAACCCATGCCTACACC
mm4__chr2 130.85 Mbp_chr2_130847128 CTGTGTGGATTCCTGACGCACTGGGTCCAGTCAAGGCGACCCTGAGGGCCTTTCCCAGCCTTGCCCCTGCCGGACACCTG
GCCCCAAAGCCGGCTGCACCCTCCAGAAGCAGGCTACCCTTC
mm4__chr4 8.50 Mbp_chr4_8501198 GGTCGTGCCACTGCTCAGTGGATCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGACAG
CAGAGGTGATGGCTTGTGCATGTGCAGGGCGGGAGGTGGCCTGTGGTGGAGCTGGCAG
mm4__chr1 111.86 Mbp_chr1_111856568 CTGTCTCTCTCAGCCTCCCTCAGGCCAGACCCCCCCTTGCACATGGTGGATCCTGAGCCTCTGCCCGACTTGGGGTCAGC
GCCAGGAAGCCTGCTGCCAGCCCCACCTGCCAAAGGTGACTGCTGGCTA
mm4__chr11 123.43 Mbp_chr11_123430828 CAACATGATGGAGAACCGGCCGCTGCCCGCCCTGCAGGTGCCCGAGCCGCAGGGTGCGCCCGAGGGCAGCCCGGTCTGGT
CCAGTTCGTCGAC
mm4__chr17 16.48 Mbp_chr17_16481570 TGAGTCCACTGTAGGCCCTTCCATAGGAATGCAGAGCTTCAATCTTCCACACCTGGGCCCCGGCAGGTGCACTGCAGGAG
GATTCACTGGCTACCCCCTGCCTCCCCCATGATGGCCTGGCAGGAGAAGGTGTCTG
mm3__chr16 49.42 Mbp_chr16_49418519 GAACAGCGACTCTCACTGCTAGGGAAGGGGCTCCATGGTCACCCGCCTTCCACATGCCAGAGACAGACCTGAGAAGGGAA
GGAGCCCAAGCCAGTGGCTCACACAACCCCAAAAC
mm4__chr7 149.44 Mbp_chr7_149436660 GCATCTGCTTCAGTTCGCTGAACTCGCTGGAGCCTTCCGTGGACTCCTCCAGCGTGTTCTCTGGCTCCTGCATGTCCAGG
TCTGGCTGTTCCCCACAGGGGTCACCCAGCTCTGAGTCCTGGAAGC
mm3__chr11 82.60 Mbp_chr11_82602646 AGACTCAGCATTGACAGCCATTTTCTGCCTTGCACATGCTGGTTCCTAAGAAATGTGAGCTCCTCTTAAGAAACAAGCAC
mm4__chr19 5.15 Mbp_chr19_5151470 CTACGTGGCCTTCGTGGAGAGCCTCCTGCAGGTGCAGGGCCGGCCCGGAGCCCCCTTCTAGGACAGCTGGCCGCTCAGGC
GACCCTCAGCCCGGCGGGGAGGCCATGGCATGCCCCGGGCGTTCGCTTGCTGTGAATT
mm4__chr5 61.63 Mbp_chr5_61626510 GATGCAAATGGCTTCCCAGCCACCCGCCAGCCCTGCCCATGCCAGCGCCTCGCGGGGTGGCTCCCGCCTCGGCGCAGCCC
CCCTGGTTGCTGGCTGCACATCTGCTTCCGAGCCACCCAGGGGCAACTTGAGGCAATGTC
mm4__chr17 8.15 Mbp_chr17_8150585 CCGTTGGACTCATTGCCACTTGAACCATTGCTGTTGGCATCGGTGTCATCGGCCAGGCTGGGGCCTGGGCAAGGCCGGTG
CTGTGGGGGCCCAGGGGATGGGACGCCCCCAGGACAAAATGATTCCCCAGGCCTGG
mm4__chr6 169.65 Mbp_chr6_169648284 TCTACAGATGCCTTCCGTGCACATGCCAATGCCTTCCGTGCACACGCCCGAGCCCTGGGTGGCATTTGGGGCCTCGCCAC
CACTGCTACCCAGCAGCTCCCAGCAGAAGGCCCAAGAGAGCCAAATGCAGAAGAGCCAG
mm4__chr6 168.90 Mbp_chr6_168895719 CAGGCTGGCCTTTGTCTAGGGCCTGCCTTACATCTGCCGGAGTCCAAAGTCCACAATTCTTCCCTTTCGATTTCTGCCTT
TTCCATAACTGGCACTGACCAGCAGAAACAGAAATGTGTTGTGAGGTCACCACCATCACC
mm4__chr1 942.30 Kbp_chr1_942302 CAGACCCCAGGTGAGGAGGCGGGTGCGCATCCCCTGGGAGCCCGCGTGGAGGCTCGCGGACCCGGCCCTGCCCCTGTCGG
AGCCGAGACGGACCGGGTAGGGGATTGCAAAGGG
mm4__chr10 29.08 Mbp_chr10_29080312 GTGAAGCAGAGAGGAGCCTGGGAACACCTCTGCTCCTGAAGTTTCCAAGATGTTCAGGGAAGAGCTACAGCCCTTCCCAG
ACCTGCCCTGCCCAGACCGGAGCCACCAACATGTGAAGCAAAGCTGCAGC
mm4__chr16 84.30 Mbp_chr16_84303327 TATTGGTCTGGCCGCTAAGCTGCTGGAACTTTCCAAAGAAGGGTAAAAGCTCCTGCATGTGGAAGGAAGGGGGTGGTGAG
CACTGAGGGAGGCATGTGGGTAGCTTT
mm4__chr11 84.72 Mbp_chr11_84720509 CATGGAGCGACAGCCTAGACCGAGCTGCCGCTTCGATCACTGCCCCCTGCACCCGCCGTGGCCATCCCGGAGCCCCGGTG
GAAGCAACGCCGCGGGCAAGTACCGAGCCTCTC
mm4__chr11 70.82 Mbp_chr11_70820987 GTGGGGTCTGAGCTTCCATCTGGCCTGGGAGACGTCCGGCTGGCTTGGGCATGTGTAAGGACGGGCCACCCCATCACACA
AAGCAGAGAGGGCACTGA
mm4__chr3 195.88 Mbp_chr3_195875665 CAGCCCTTCCTTCCAGGCCCGCCCTGTCCGCGCCCTCTCTCTCCCGCCATGAACAGGCCGGAGTCCCCGTGGCCATGGGA
GGGCCAGGGCTGCTACCCGGCCGTGACTGAAGCAGGGGCGGATGAGGACTTCCTG
mm4__chr1 149.01 Mbp_chr1_149008369 GACACAAGCTGAGGTATCCCAGAATAACTGTTCTGGATGCACACATGAAATGCTCACTACTCTGTCTCCAGATAGGCTCC
TGCATTTGTAATGCGGGAAGTGGGACCAGCCTAATTTCT
mm4__chr1 143.54 Mbp_chr1_143542937 GACACAAGCTGAGGTCTCCCAGAATAACTGTTCTGGATGCACACATGAAATGCTCACTACTCTGTCTCCAGATAGGCTCC
TGCATTTGTAATGCGGGAAGTGGGACCAGCCTAATTTCT
mm4__chr1 120.51 Mbp_chr1_120513868 AGAAATTAGGCTGGTCCCACTTCCCGCATTACAAATGCAGGAGCCTATCTGGAGACAGAGTAGTGAGCATTTCATGTGTG
CATCCAGAACAGTTATTCTGGGATACCTCAGCTTGTGTC
mm4__chr14 72.64 Mbp_chr14_72642726 CATGCCCCTGTCTCACTCTCACCCTGCTCTGCCTTCCTTCCACCTGCCTGAGCCTCTCTCACCTCCAACAAGCCTCCCCT
TTAGGTTCCCCAGAACACAACCCCAGTGT
mm4__chr1 21.90 Mbp_chr1_21896695 GGACACCCAGGACATGAGTGAGTGGATGGGGGGATGCGGGCGGGTAAAGGGGTGGCCTCGGGCAGCGCAGATGGGGGGCA
CAGGCAGGTGGAAGGCTGGCCAGGGTCAGCATGGGTGGGGACACAGGCAGAAGTCAA
mm4__chr20 62.49 Mbp_chr20_62493808 GTGAGGGTGGACCAGATTCCCCGGGTGGCCTCGGCAGGTGGAAGGCAGGGACCTCCATGCATGGACTGAGTGCCT
mm4__chr6 151.01 Mbp_chr6_151007648 TGTGCTAACCTGCAGGACAGCGCCGCATAATCCAGTACTGGGGCCTTATTGTCCAGCCTTCCACCTCCAGGAGCCCACTC
AGTGATGACTTTTGACAAAAGTCACCCGGATTATCCCTCTTTTGGGGTCCATGATGCCA
mm4__chr5 177.49 Mbp_chr5_177489734 GTGACTCTAAAGGGGTGCCCAGGGACCCCAAAGGACGGGGGTGGAGCCCCAGGCAGCTGAGAGAAGCCCACCACCCCCAT
GCCCCTGGAGGCTGCCCACCCTTGCCCAGGCCCGAGCCCACTCCCTCTCACCAGGCCA
mm4__chr7 63.11 Mbp_chr7_63113867 GGAGGAGGACTTGGACAGGGCCTGGCTGGCACAGGCCGCAGCCAGCAGCTCCCTCAGGCACCAGGCCACCTCCTGTACCT
TGGGCAGTAGC
mm4__chr7 36.29 Mbp_chr7_36286588 CTGTGTTTCTGCACTTGCCCTTGGGGATGCAGGGCTAGGGCATGTCCTAGGCTGGCCAGGTTCTGTAAGCTTTTGGTTTG
GACACGTTTGAAATTAGGTTAAAGTCAGCATCTGGCTATAGTGGGCTCCAGA
mm4__chr7 149.73 Mbp_chr7_149725176 CTCCCCTCCTGTCTTCATGCCTTCCAGGGAGCAGGGTGGGGGGCGAGAGCCGATCAGTCTCCCACAGAAGGGTTTCCTGC
CTGGCAGGTGCCAGAGCCTGGTACAGGACTCGTCACCCCTGTAGAAAGCACGT
mm4__chr6 109.44 Mbp_chr6_109443795 CTGTGGGCAGGTGCATTCTACCTCTTCCACGTACAGGAGGTCAATGGCTTATATAGGGCCCAGGCTGAGCTCCAGCATGT
GCTAGGAAGGGCAAGGGAGGCCCAGGATCTGGGCCCAGAGCCTCAGCCAGCCCTACTCCT
mm4__chr20 46.17 Mbp_chr20_46174449 GGGGAAACGCGTTGTCCTGGGGCCCCGGCGTGTGCTGGGCGGGTGAGCAGCCGCGCCCCGACGGCAGGGCGAGGGGCTAG
GAG
mm4__chr2 86.26 Mbp_chr2_86255089 AGCAAACCAGGAACACAGGTTCTCTCCAGCAATCACCCCCGTGGCCCTGCCTGGCACAGGCTGGAGCTTCCTGCTGGGCT
GTGCTCATTTA
mm4__chr2 42.37 Mbp_chr2_42369066 TTTACCATTCTGGGCGGGTCTCACTTTATTGTGTTTTCTCCCACTGTTTTTGGCTTCCCTGCATTGTCCTGCCTGGCACA
CGCCCCAGCCATGTTCTCTGTGGCCTATCACCGTGATG
mm4__chr19 18.85 Mbp_chr19_18851906 CAGGTTCACCCAAGTACCGGAACCCCGCTGGGGTGCGGAGCAGCAGCTGAGGCCCAGGCATGGGCCAGGCCGGTGTGCAG
CTCCCTGTGTGGCATGGAGTTCCCGTTCCCAG
mm4__chr16 57.91 Mbp_chr16_57911224 ACAGCGTATTTGCACACCCTAATAGAGTTTGGATGCAGAAAGGATCCAAGCAAGTCTTGTTCACACCAGTACCTCCGGCC
TGGCACATGCAGGTGCTCAGGAAACCTTAGCTTGGGGAAGATGTAGTGACCTGGC
mm4__chr16 55.33 Mbp_chr16_55327338 GGAGGCTGCAGGGAGTTTTACATCCAGCCTGGCACAACCAGGAGCCTATTATCCCTATGAGCGGACTCTGGGGCAGTACC
AATATGAACGGTAAGGAGTGAAGGGCCTTGGTGACTGGCACTGTGA
mm4__chr16 51.26 Mbp_chr16_51255816 AACAGCAGACTCCTGGAAGGGAGAGAAAGTCTAGCCTAATGAGATCTCTCGCTCCTGCAAGTGCCAGGCAGGCCAGTCTC
AGTTCAGCCATTTCCAAGTTGCT
mm4__chr16 12.67 Mbp_chr16_12674312 CCTCCTGGGTTCTGATGCTGAGTTCAAGGCTCTGGCATGTTCCTGGCTGGCTCAAGGAGTCTGAAACGAGGAATGAGATG
TTGAGCTGGAAAATGGGGGAAACACTTCTTGGGTTGGAGCCAG
mm4__chr16 1.20 Mbp_chr16_1202215 CATGCCGACTGCCACATAGAGGGGCCGCAGGAGAGGGCCCGGGTGGCACATGCCGCAGCCACTGCCGCTGCCAGCCTCAG
ACTGGCCACAGGGCTGGGCACCATGAACTACCCCACGATCCTGC
mm4__chr12 53.72 Mbp_chr12_53722053 CTGTTCCATCAGCTCCGTGTGCTCCTGCCTGGCAGTTGCCAGAGCCCTCTCGAGCTCCTGCACTCGGCTCCTCAGCTCTG
TCACCTGTCCCTCCAGCTGTAGCTTCAGCTGCATCAGGTCATTCCGTTCTTGCTGGCT
mm4__chr10 62.40 Mbp_chr10_62397056 GTTGATGCCAGTGCCTTTGGTTCCCCAGAAGCTACCAGCCTGGCGTTTGCCGGAGCCATCTGTCCCACCTTCTTTGTATG
TTGCATAATCTTTGGGACCTCATGTGATAACGGTGCCT
mm4__chr10 132.54 Mbp_chr10_132537008 CTGCGAGGGTTCCCTGCACGCCCCGCGCTGAGACCCAGCCTGGCCCGGGCCGGAGCCCTCCGCGACGTCAGGATGCGCCT
GGCCTCACGCCGCCTCCCCTCCGTCTTCTCTG
mm4__chr1 46.51 Mbp_chr1_46508158 TGAGCAGCTGGTAAACCCACATGGGCTCAGCCCTGTGCCAGGCAGGCCTCAGAAAACTTCAGGGAACTCCTAGGGAGAAC
AGGTGCCCATGACCCATGAGAGGGCACAACAGGAGGGTATC
mm4__chr1 228.22 Mbp_chr1_228219110 GTCTGCGAAGGACACTCAGGCCACCAGGCCCTATTGATAGCTTCTCGTAGGCACACGCCCTGCCCTCCTGGCACATGCCA
GGGCCCTCACTGGGCAGGGGTTGGGCATCACAGCTGCCTGTGCATGGGAAGAGCTACG
mm3__chr19 48.49 Mbp_chr19_48490780 TCTCTCTCCTTGTCTGCCCCTGAGCACCGTGTTCCCCAAACGGAGGTGGGATCAGGATCCGGCAAGTGCCAGGCCGGAGT
CTGAGCCCAGAGATGGGACAAAGATGGGCAAGAGTCGGG
mm3__chr10 47.97 Mbp_chr10_47971772 GAATGGTGTGGAAAGGCCCTGCCCAAGAAGGCTGGATGCTGGCTGGGGAATGCCCAGGGTGCCAGCTCCCTACCCTGGGG
AAAGGCCAGGCTGGCATATGCCGGAGCCAGGGTCCAAGGTCACAGAGAGGC
mm3__chr10 46.41 Mbp_chr10_46412860 GCCTCTCTGTGACCTTGGACCCTGGCTCCGGCATATGCCAGCCTGGCCTTTCCCCAGGGTAGGGAGCTGGCACCCTGGGC
ATTCCCCAGCCAGCATCCAGCCTTCTTGGGCAGGGCCTTTCCACACCATTC

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.