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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529751_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529751_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm3__chr7 47.16 Mbp_chr7_47156107_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm3__chr7 47.16 Mbp_chr7_47156107_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm3__chr20 22.27 Mbp_chr20_22270044_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm3__chr20 22.27 Mbp_chr20_22270044_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4__chrY 1.66 Mbp_chrY_1656064_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chrY 1.66 Mbp_chrY_1656064_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chrX 6.68 Mbp_chrX_6676882_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chrX 6.68 Mbp_chrX_6676882_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chrX 52.54 Mbp_chrX_52541094_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chrX 52.54 Mbp_chrX_52541094_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr9 12.38 Mbp_chr9_12380896_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr9 12.38 Mbp_chr9_12380896_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr7 3.13 Mbp_chr7_3129539_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr7 3.13 Mbp_chr7_3129539_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr4 557.32 Kbp_chr4_557316_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr4 557.32 Kbp_chr4_557316_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr4 158.15 Mbp_chr4_158151931_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr4 158.15 Mbp_chr4_158151931_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr21 13.24 Mbp_chr21_13237156_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr21 13.24 Mbp_chr21_13237156_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr20 55.94 Mbp_chr20_55936884_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr20 55.94 Mbp_chr20_55936884_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr2 131.92 Mbp_chr2_131921381_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr2 131.92 Mbp_chr2_131921381_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4__chr7 110.73 Mbp_chr7_110730235_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4__chr7 110.73 Mbp_chr7_110730235_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4__chr16 5.42 Mbp_chr16_5419452_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4__chr16 5.42 Mbp_chr16_5419452_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4__chr13 66.18 Mbp_chr13_66184666_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4__chr13 66.18 Mbp_chr13_66184666_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4__chr4 5.27 Mbp_chr4_5270417_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr4 5.27 Mbp_chr4_5270417_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr4 115.64 Mbp_chr4_115639101_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr4 115.64 Mbp_chr4_115639101_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr3 130.86 Mbp_chr3_130858145_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr3 130.86 Mbp_chr3_130858145_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr15 97.05 Mbp_chr15_97053673_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr15 97.05 Mbp_chr15_97053673_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr15 47.40 Mbp_chr15_47396660_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr15 47.40 Mbp_chr15_47396660_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr3 3.78 Mbp_chr3_3782864_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr3 3.78 Mbp_chr3_3782864_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr9 135.18 Mbp_chr9_135181841_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr9 135.18 Mbp_chr9_135181841_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr8 104.27 Mbp_chr8_104268622_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr8 104.27 Mbp_chr8_104268622_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr7 10.55 Mbp_chr7_10551082_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr7 10.55 Mbp_chr7_10551082_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr12 4.80 Mbp_chr12_4795469_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr12 4.80 Mbp_chr12_4795469_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr11 56.15 Mbp_chr11_56153244_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr11 56.15 Mbp_chr11_56153244_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr10 57.13 Mbp_chr10_57134980_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr10 57.13 Mbp_chr10_57134980_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr1 220.83 Mbp_chr1_220829897_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr1 220.83 Mbp_chr1_220829897_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4__chr20 46.58 Mbp_chr20_46581180_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4__chr20 46.58 Mbp_chr20_46581180_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4__chr16 10.56 Mbp_chr16_10563895_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4__chr16 10.56 Mbp_chr16_10563895_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4__chr9 129.26 Mbp_chr9_129263907_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr9 129.26 Mbp_chr9_129263907_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr3 69.76 Mbp_chr3_69760303_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr3 69.76 Mbp_chr3_69760303_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr16 31.73 Mbp_chr16_31727300_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr16 31.73 Mbp_chr16_31727300_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr12 84.20 Mbp_chr12_84195560_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4__chr12 84.20 Mbp_chr12_84195560_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4__chr8 144.28 Mbp_chr8_144276255_F | TCGTCGGCAGCGTCCAGAGCTGTCCTCCGCATAC | 60.2 | 0.21 |
| mm4__chr8 144.28 Mbp_chr8_144276255_R | GTCTCGTGGGCTCGGTCTCTCTCTCTCTGCAGGCC | 60.3 | 0.21 |
| mm4__chr19 48.42 Mbp_chr19_48415408_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4__chr19 48.42 Mbp_chr19_48415408_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4__chr9 126.61 Mbp_chr9_126611236_F | TCGTCGGCAGCGTCCAGACCAGAGAAGGCAGCC | 60.0 | 0.13 |
| mm4__chr9 126.61 Mbp_chr9_126611236_R | GTCTCGTGGGCTCGGGCTCCTGCCAGTCATATCCC | 59.9 | 0.13 |
| mm4__chr1 154.94 Mbp_chr1_154935008_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr1 154.94 Mbp_chr1_154935008_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm1__chr6 170.17 Mbp_chr6_170166981_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm1__chr6 170.17 Mbp_chr6_170166981_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr8 13.79 Mbp_chr8_13785409_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr8 13.79 Mbp_chr8_13785409_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr10 109.36 Mbp_chr10_109362968_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr10 109.36 Mbp_chr10_109362968_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr5 132.75 Mbp_chr5_132747274_F | TCGTCGGCAGCGTCGATGGAGGGAAGGGAGGGG | 60.7 | 0.08 |
| mm4__chr5 132.75 Mbp_chr5_132747274_R | GTCTCGTGGGCTCGGGAGCTACCTCTGGCGCAC | 60.2 | 0.08 |
| mm4__chr11 1.47 Mbp_chr11_1473793_F | TCGTCGGCAGCGTCCACATGTGGACTCACGCATTC | 59.8 | 0.08 |
| mm4__chr11 1.47 Mbp_chr11_1473793_R | GTCTCGTGGGCTCGGGTGTGAGGGGCCACGTAA | 59.2 | 0.08 |
| mm4__chr11 134.53 Mbp_chr11_134531372_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr11 134.53 Mbp_chr11_134531372_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr1 225.43 Mbp_chr1_225428067_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr1 225.43 Mbp_chr1_225428067_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3__chr17 10.20 Mbp_chr17_10198491_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3__chr17 10.20 Mbp_chr17_10198491_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr11 62.65 Mbp_chr11_62646529_F | TCGTCGGCAGCGTCGAGTGGAATGGGACTTGGGG | 60.0 | 0.06 |
| mm4__chr11 62.65 Mbp_chr11_62646529_R | GTCTCGTGGGCTCGGGGAGGTTTGGGGTCAAGGAG | 59.9 | 0.06 |
| mm3__chr11 95.15 Mbp_chr11_95150806_F | TCGTCGGCAGCGTCCGGCCGGGAGCTATTACAG | 60.0 | 0.06 |
| mm3__chr11 95.15 Mbp_chr11_95150806_R | GTCTCGTGGGCTCGGTGAGAGAGGCCAGCAGGTAT | 60.0 | 0.06 |
| mm4__chr1 236.14 Mbp_chr1_236142233_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr1 236.14 Mbp_chr1_236142233_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr9 129.38 Mbp_chr9_129383639_F | TCGTCGGCAGCGTCGAGTGGCAGGCCCTCCTC | 61.7 | 0.05 |
| mm4__chr9 129.38 Mbp_chr9_129383639_R | GTCTCGTGGGCTCGGAGACAGCTCGGAAACTGACG | 60.0 | 0.05 |
| mm4__chr16 49.59 Mbp_chr16_49590807_F | TCGTCGGCAGCGTCGATGAGGTCCAGCTGGCAAA | 60.3 | 0.05 |
| mm4__chr16 49.59 Mbp_chr16_49590807_R | GTCTCGTGGGCTCGGATGGGTGACTTTCCCCTCCT | 60.1 | 0.05 |
| mm3__chr22 48.66 Mbp_chr22_48658086_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3__chr22 48.66 Mbp_chr22_48658086_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr3 181.72 Mbp_chr3_181719478_F | TCGTCGGCAGCGTCCAGTGCCCAGTGCTGAGA | 59.2 | 0.05 |
| mm4__chr3 181.72 Mbp_chr3_181719478_R | GTCTCGTGGGCTCGGACACAGCACCTTCTTGGGG | 59.8 | 0.05 |
| mm4__chr22 30.29 Mbp_chr22_30289365_F | TCGTCGGCAGCGTCGCGATCCTAATCCTCCGACC | 59.7 | 0.04 |
| mm4__chr22 30.29 Mbp_chr22_30289365_R | GTCTCGTGGGCTCGGATCAAGCTGCTCTTCCTGCC | 60.3 | 0.04 |
| mm4__chr19 46.39 Mbp_chr19_46387240_F | TCGTCGGCAGCGTCAGTGCATCAACGGCAAAGTG | 59.6 | 0.04 |
| mm4__chr19 46.39 Mbp_chr19_46387240_R | GTCTCGTGGGCTCGGCCCAACTTCCTTCACCAGCA | 60.1 | 0.04 |
| mm4__chr1 44.81 Mbp_chr1_44813243_F | TCGTCGGCAGCGTCGACGAGGCTGAACTGGTCC | 60.0 | 0.03 |
| mm4__chr1 44.81 Mbp_chr1_44813243_R | GTCTCGTGGGCTCGGCCTCCTTCCGTGCCCTTG | 60.0 | 0.03 |
| mm4__chr14 91.25 Mbp_chr14_91253768_F | TCGTCGGCAGCGTCGAGAGAAAGGGGTTGGCTCG | 60.3 | 0.03 |
| mm4__chr14 91.25 Mbp_chr14_91253768_R | GTCTCGTGGGCTCGGAAGGGACCCGAAGACCTCC | 60.6 | 0.03 |
| mm4__chr22 50.46 Mbp_chr22_50457151_F | TCGTCGGCAGCGTCCAGCAGAGAGAAGGCTCAGG | 59.8 | 0.03 |
| mm4__chr22 50.46 Mbp_chr22_50457151_R | GTCTCGTGGGCTCGGCTTGGTGGAACCCTCTGACC | 59.9 | 0.03 |
| mm3__chr19 1.59 Mbp_chr19_1592774_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3__chr19 1.59 Mbp_chr19_1592774_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr10 930.92 Kbp_chr10_930922_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr10 930.92 Kbp_chr10_930922_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr22 42.34 Mbp_chr22_42341042_F | TCGTCGGCAGCGTCGCTCCCAATTAGCACACAGG | 58.9 | 0.02 |
| mm4__chr22 42.34 Mbp_chr22_42341042_R | GTCTCGTGGGCTCGGCAAGGAGCAGAGGCCAGG | 59.7 | 0.02 |
| mm4__chr2 232.87 Mbp_chr2_232871603_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr2 232.87 Mbp_chr2_232871603_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr4 52.66 Mbp_chr4_52658959_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr4 52.66 Mbp_chr4_52658959_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr1 10.69 Mbp_chr1_10694152_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr1 10.69 Mbp_chr1_10694152_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr5 134.53 Mbp_chr5_134527676_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr5 134.53 Mbp_chr5_134527676_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr4 8.16 Mbp_chr4_8159401_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr4 8.16 Mbp_chr4_8159401_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr9 137.45 Mbp_chr9_137452667_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr9 137.45 Mbp_chr9_137452667_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr11 120.34 Mbp_chr11_120336379_F | TCGTCGGCAGCGTCGGAAACCAGAACCGGAACCG | 61.2 | 0.00 |
| mm4__chr11 120.34 Mbp_chr11_120336379_R | GTCTCGTGGGCTCGGCTCGCCGGGTTCTGGAGG | 62.1 | 0.00 |
| mm4__chr21 44.01 Mbp_chr21_44012824_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr21 44.01 Mbp_chr21_44012824_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 169.89 Mbp_chr1_169893685_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 169.89 Mbp_chr1_169893685_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr19 47.23 Mbp_chr19_47230542_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr19 47.23 Mbp_chr19_47230542_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr13 24.68 Mbp_chr13_24680444_F | TCGTCGGCAGCGTCTCAATCCTGGACTCTCCCGA | 59.6 | 0.00 |
| mm4__chr13 24.68 Mbp_chr13_24680444_R | GTCTCGTGGGCTCGGCACCAGCCTTAAATGCCGC | 59.8 | 0.00 |
| mm4__chr9 13.28 Mbp_chr9_13279333_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr9 13.28 Mbp_chr9_13279333_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr9 124.51 Mbp_chr9_124505065_F | TCGTCGGCAGCGTCCCCTCTATATCGGCGCGTG | 60.1 | 0.00 |
| mm4__chr9 124.51 Mbp_chr9_124505065_R | GTCTCGTGGGCTCGGGAAGACTCGAGCTGGACTGC | 60.4 | 0.00 |
| mm4__chr7 155.64 Mbp_chr7_155644664_F | TCGTCGGCAGCGTCCTCCATCATCCCAGGGTGC | 59.8 | 0.00 |
| mm4__chr7 155.64 Mbp_chr7_155644664_R | GTCTCGTGGGCTCGGGTCCACAGCTGGCCCTTC | 60.3 | 0.00 |
| mm4__chr6 107.11 Mbp_chr6_107114712_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr6 107.11 Mbp_chr6_107114712_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr13 54.16 Mbp_chr13_54156577_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr13 54.16 Mbp_chr13_54156577_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr11 113.45 Mbp_chr11_113452473_F | TCGTCGGCAGCGTCTCCATGTTGAGGAGCAAGGG | 59.6 | 0.00 |
| mm4__chr11 113.45 Mbp_chr11_113452473_R | GTCTCGTGGGCTCGGGGGTGGATCTGAATCAGCAGT | 59.7 | 0.00 |
| mm4__chr10 58.32 Mbp_chr10_58320852_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr10 58.32 Mbp_chr10_58320852_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 193.53 Mbp_chr1_193527171_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 193.53 Mbp_chr1_193527171_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr1 32.01 Mbp_chr1_32009194_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr1 32.01 Mbp_chr1_32009194_R | Primer3: not found at this Tm | N.d. | 0.00 |
Primers underlined, off-targets in bold.
| mm4__chr8 144.28 Mbp_chr8_144276255 |
CAGAGCTGTCCTCCGCATACTCATCCCCAATCCGGGCGCTCGCCATCTCTGTCCTCGGGCAAGGAGTGCTGGCCTGCAGA GAGAGAGAGA |
| mm4__chr9 126.61 Mbp_chr9_126611236 |
CAGACCAGAGAAGGCAGCCAGGCTCTGGCGTCCACCGAGCCAGCGCCCCTATCTGGGCGCGGGCCGCATGGCGGCAGACC ACCCTATATCCGCCTTGGTGGCGTGGGATATGACTGGCAGGAGC |
| mm4__chr5 132.75 Mbp_chr5_132747274 |
GATGGAGGGAAGGGAGGGGGTCCGCCACCCGCCTGTGCGCGCCCAGTCCTGCCGCATCCTGGGTGCGCGCCGCCCACCCG CGGGGCGAGTGCCCAGGAGGCGAGGCGCGGCCCGGTGCGCCAGAGGTAGCTC |
| mm4__chr11 1.47 Mbp_chr11_1473793 | CACATGTGGACTCACGCATTCATAACCACAATCAGGACACGCGGCGGCTTACGTGGCCCCTCACAC |
| mm4__chr11 62.65 Mbp_chr11_62646529 |
GAGTGGAATGGGACTTGGGGGATCTGGGGGCGTCCCGGGCGCGCCCCCAGATTCCGGGCTCCTCANNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNATCTTGTGCAGAGTTTGCTCCTTGACCCCAAACCTCC |
| mm3__chr11 95.15 Mbp_chr11_95150806 |
CGGCCGGGAGCTATTACAGCAGGGTCTGCCCGTCCGACGACGGCGGCTCCAATCTGGGGTCCCGCCGGCGCCGACCCCTC AGCTCTCTCCCGATACCTGCTGGCCTCTCTCA |
| mm4__chr9 129.38 Mbp_chr9_129383639 |
GAGTGGCAGGCCCTCCTCGGTGGCCGCCGAGCGCGCGCCCAGCTTGGGGAACGCGCTGCCTGCGCACCCGGGCGCGCCCG GGAAGGCCGGCTCGCTGGCACCGCGTCAGTTTCCGAGCTGTCT |
| mm4__chr16 49.59 Mbp_chr16_49590807 |
GATGAGGTCCAGCTGGCAAACTGAAGCGATGCCAGCATCCTCACACCACAACCTGGGCTCCAGCCGGCCCGGTGAGGGCC TTTCCCCCACCACCACTCTGGCCACTCTGTCTCCAGAGAAGGAGGGGAAAGTCACCCAT |
| mm4__chr3 181.72 Mbp_chr3_181719478 |
CAGTGCCCAGTGCTGAGAGCCGACGCGCGGGCAAATTGAGGCCGAGCTGACGAGCTCCGGCGGGTGGACCTGACGTCACC GCGGCCCGGGTCACCTCACCCATGGGGCTCCCCAAGAAGGTGCTGTGT |
| mm4__chr22 30.29 Mbp_chr22_30289365 |
GCGATCCTAATCCTCCGACCCTGGCCCGGAGCGAGAGCAGAGCCCCCAGCCCCTATCTGCGCTCCCGAACCCGGGCGCGC ACCGGCTGCGGCGGGGCAGGAAGAGCAGCTTGAT |
| mm4__chr19 46.39 Mbp_chr19_46387240 |
AGTGCATCAACGGCAAAGTGCTGGTGAGGACGGCGCGAGCCCTGAGTGTGGGTTCCCCACCCAGCCACACCTGGGCAGAT GTGCTGGTGAAGGAAGTTGGG |
| mm4__chr1 44.81 Mbp_chr1_44813243 |
GACGAGGCTGAACTGGTCCGCGCGGCCCGAAGCTGGGCGCGCGTGGGCGCGGTGAGTGGGGCTGGGGGAGCGCAAGGGCA CGGAAGGAGG |
| mm4__chr14 91.25 Mbp_chr14_91253768 |
GAGAGAAAGGGGTTGGCTCGCGGGGACACCTACCTGCGCGGCGCGGGGCTGTGGCGCCGGCCACCATTACCCCGCGCCCC TCCTCTTGCCAGCGCGCACGCAGATGGCGGGGTGGCCTGGGGAGGTCTTCGGGTCCCTT |
| mm4__chr22 50.46 Mbp_chr22_50457151 |
CAGCAGAGAGAAGGCTCAGGCCTAGCCCCAGGCCTGGGCGTGCCCAGCTTGGGGGTGCCTACAGGTCAGAGGGTTCCACC AAG |
| mm4__chr22 42.34 Mbp_chr22_42341042 |
GCTCCCAATTAGCACACAGGTCCTCCATCAAAGCGCGCGCCGGCTGCCAGGAGCAGAGGCCGGAGGGACGGCCCTGCTAG CAGCAGGAGGAGGCCGTGGCTCTCTGGCAAGCCCTGGCCTCTGCTCCTTG |
| mm4__chr11 120.34 Mbp_chr11_120336379 |
GGAAACCAGAACCGGAACCGGAACCCGCGCGCGCGCCCGCGGTTGCGCGGCCCCCACGGCCGCCGGCCTCCTCCGCTGTC TGCGCGCGCGCCCGCGAGGGCCTCCAGAACCCGGCGAG |
| mm4__chr13 24.68 Mbp_chr13_24680444 |
TCAATCCTGGACTCTCCCGACCCCTAGCTGTCGGTCCCCCTCCCTGCGCGCGCGCCGGCGGGTTTCCTACCCTCCGAGGC GTCCGCTGGCCTGCGCCCTGGCGGGGACGTGGGCGGGGCGGGCGGCATTTAAGGCTGGTG |
| mm4__chr9 124.51 Mbp_chr9_124505065 |
CCCTCTATATCGGCGCGTGCGGTATCCGGGGGCGGAGGCCCGGTCGTGTCCTCCCCCCAAGCCCGGCCCGCGCCGGCACC CTCTCCGCAGTCCAGCTCGAGTCTTC |
| mm4__chr7 155.64 Mbp_chr7_155644664 |
CTCCATCATCCCAGGGTGCACCGCGGCGGGCGCGGGCGCGCGGCCATGTTGCGGTAGTTTGTTGTTTTCTTCCTGCGGAG GCGAAGGGCCAGCTGTGGAC |
| mm4__chr11 113.45 Mbp_chr11_113452473 |
TCCATGTTGAGGAGCAAGGGCTAGGGCCACTGGTGTGCATGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNACATTGGGGGACAGGAAATGAGACACTGCTGATTCAGATCCACCC |