CRISPOR

Micro-homology scoring of potential deletions

Guide: CCGAGTCCTTAGGCCGCCAG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	(Wild-type sequence)
485.6	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCC----------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`10 bp deleted → frameshift`
417.6	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCG-------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`13 bp deleted → frameshift`
346.2	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC-----------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`11 bp deleted → frameshift`
329.4	`CCCTGGCCACTTCCGGCGCGC----------------------------------GCCCAGATTGGGGACAAAGGAAGCC`	`34 bp deleted → frameshift`
327.6	`CCCTGGCCACTTCCGGCGCGCCG--------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`26 bp deleted → frameshift`
294.4	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCC--------------------CAGATTGGGGACAAAGGAAGCC`	`20 bp deleted → frameshift`
269.4	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC----------------GCGCCCAGATTGGGGACAAAGGAAGCC`	`16 bp deleted → frameshift`
268.0	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC--------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`8 bp deleted → frameshift`
256.2	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC-----------------GCGCGCCCAGATTGGGGACAAAGGAAGCC`	`17 bp deleted → frameshift`
255.2	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG---------GGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`9 bp deleted → no frameshift`
247.0	`CCCTGGCCACTTCCGGCGC----------------------------CGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`28 bp deleted → frameshift`
245.0	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG---------------------ATTGGGGACAAAGGAAGCC`	`21 bp deleted → no frameshift`
244.2	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC------------------GCCCAGATTGGGGACAAAGGAAGCC`	`18 bp deleted → no frameshift`
242.8	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG----------GCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`10 bp deleted → frameshift`
231.0	`CCCTGGCCACTTCCGGCGCGCC------------------------------------CAGATTGGGGACAAAGGAAGCC`	`36 bp deleted → no frameshift`
198.8	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC--------------GCGCGCCCAGATTGGGGACAAAGGAAGCC`	`14 bp deleted → frameshift`
190.2	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCC-----------------------CAGATTGGGGACAAAGGAAGCC`	`23 bp deleted → frameshift`
161.6	`CCCTGGCCACTTCCGGCGCGC--------------------------------GCGCCCAGATTGGGGACAAAGGAAGCC`	`32 bp deleted → frameshift`
154.8	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC-------------------GCGCCCAGATTGGGGACAAAGGAAGCC`	`19 bp deleted → frameshift`
147.2	`CCCTGGCCACTTCCGGCGCGCCGAGTCC--------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`20 bp deleted → frameshift`
140.0	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC---------------------GCCCAGATTGGGGACAAAGGAAGCC`	`21 bp deleted → no frameshift`
126.8	`CCCTGGCCACTTCCGGCGCGCCG-----------------------CCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`23 bp deleted → frameshift`
120.4	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCC------------------------AGATTGGGGACAAAGGAAGCC`	`24 bp deleted → no frameshift`
120.4	`CCCTGGCCACTTCCGGCGCGC------------------------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`24 bp deleted → no frameshift`
120.0	`CCCTGGCCACTTCCGGCGC--------------------------------------CCAGATTGGGGACAAAGGAAGCC`	`38 bp deleted → frameshift`
109.2	`CCCTGGCCACTTCCGG--------------------------GGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`26 bp deleted → frameshift`
103.6	`CCCTGGCCACTTCCGG---------------------------GCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`27 bp deleted → no frameshift`
94.0	`CCCTGGCCACTTCCGGCGCGCCG-----------------------------CGCGCCCAGATTGGGGACAAAGGAAGCC`	`29 bp deleted → frameshift`
94.0	`CCCTGGCCACTTCCGGCGCG-----------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`29 bp deleted → frameshift`
90.0	`CCCTGGC--------------------------------------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`38 bp deleted → frameshift`
89.2	`CCCTGGCCACTTCCGGCGCGCCGAGTCC------------------------------CAGATTGGGGACAAAGGAAGCC`	`30 bp deleted → no frameshift`
89.2	`CCCTGGCCACTTCCGGCGCGC------------------------------GCGCGCCCAGATTGGGGACAAAGGAAGCC`	`30 bp deleted → no frameshift`
89.2	`CCCTGGCCACTTCCGGC------------------------------CGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`30 bp deleted → no frameshift`
84.8	`CCCTGGCCACTTCCGGCGCGCCGAGTCC-------------------------------AGATTGGGGACAAAGGAAGCC`	`31 bp deleted → frameshift`
84.8	`CCCTGGCCACTTCCGGCGCGCCG-------------------------------CGCCCAGATTGGGGACAAAGGAAGCC`	`31 bp deleted → frameshift`
84.8	`CCCTGGCCACTTCCGGCG-------------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`31 bp deleted → frameshift`
84.8	`CCCTGGCCACTTCCG-------------------------------CCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`31 bp deleted → frameshift`
81.0	`CCCTGGCC----------------------------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`40 bp deleted → frameshift`
76.8	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG---------------------------------GGACAAAGGAAGCC`	`33 bp deleted → no frameshift`
76.8	`CCCTGGCCACTTCCGGCGCGCCG---------------------------------CCCAGATTGGGGACAAAGGAAGCC`	`33 bp deleted → no frameshift`
73.2	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG----------------------------------GACAAAGGAAGCC`	`34 bp deleted → frameshift`
73.2	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCC------------------------------------------`	`42 bp deleted → no frameshift`
70.5	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAG-----------------------------ATTGGGGACAAAGGAAGCC`	`29 bp deleted → frameshift`
69.6	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG-----------------------------------ACAAAGGAAGCC`	`35 bp deleted → frameshift`
66.6	`CCCTGGC--------------------------------------------GCGCGCCCAGATTGGGGACAAAGGAAGCC`	`44 bp deleted → frameshift`
66.0	`CCCTGG------------------------------------GGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`36 bp deleted → no frameshift`
63.0	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCC---------------------------------------------`	`45 bp deleted → no frameshift`
62.8	`CCCTGGCCACTTCCGGCGCGCC-------------------------------------AGATTGGGGACAAAGGAAGCC`	`37 bp deleted → frameshift`
62.8	`CCCTGGCCACTTCCG-------------------------------------CGCGCCCAGATTGGGGACAAAGGAAGCC`	`37 bp deleted → frameshift`
62.8	`CCCTGG-------------------------------------GCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`37 bp deleted → frameshift`
61.0	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG------------------------------------------AAGCC`	`42 bp deleted → no frameshift`
60.6	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCA--------------------------------AAGGAAGCC`	`32 bp deleted → frameshift`
56.8	`CCCTGGCCACTTCCG---------------------------------------CGCCCAGATTGGGGACAAAGGAAGCC`	`39 bp deleted → no frameshift`
54.9	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG----------------------------------GAAGCC`	`34 bp deleted → frameshift`
54.0	`CCCTGGCCACTTCCGGC----------------------------------------CCAGATTGGGGACAAAGGAAGCC`	`40 bp deleted → frameshift`
51.6	`CCCTGGCCACTTCCG-----------------------------------------CCCAGATTGGGGACAAAGGAAGCC`	`41 bp deleted → frameshift`
49.5	`CCCTGGCCACTTCCGGCGCGCCGAG------------------------------------ATTGGGGACAAAGGAAGCC`	`36 bp deleted → no frameshift`
49.2	`CCCTGGCC--------------------------------------------------CAGATTGGGGACAAAGGAAGCC`	`50 bp deleted → frameshift`
45.0	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG--------------------------------------CC`	`38 bp deleted → frameshift`
45.0	`CCCTGGCCACTTCCGGCGCGCCGA--------------------------------------TTGGGGACAAAGGAAGCC`	`38 bp deleted → frameshift`
44.4	`CCCTGGCCACTTCC--------------------------------------------CAGATTGGGGACAAAGGAAGCC`	`44 bp deleted → frameshift`
42.0	`CCCTGGCCACTTCC---------------------------------------------AGATTGGGGACAAAGGAAGCC`	`45 bp deleted → no frameshift`
42.0	`CCC---------------------------------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`45 bp deleted → no frameshift`
40.0	`CCCTGGC----------------------------------------------GCGCCCAGATTGGGGACAAAGGAAGCC`	`46 bp deleted → frameshift`
40.0	`CC----------------------------------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC`	`46 bp deleted → frameshift`
39.0	`CCCTGGCCA---------------------------------------------------GATTGGGGACAAAGGAAGCC`	`51 bp deleted → no frameshift`
36.6	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTT----------------------------------GGGGACAAAGGAAGCC`	`34 bp deleted → frameshift`
36.6	`CCC--------------------------------------------------------AGATTGGGGACAAAGGAAGCC`	`56 bp deleted → frameshift`
36.4	`CCCTGGC------------------------------------------------GCCCAGATTGGGGACAAAGGAAGCC`	`48 bp deleted → no frameshift`
33.0	`CCCTGGCCACTTCCGGCGCGCC----------------------------------------------------------`	`58 bp deleted → frameshift`
32.8	`CCCTGGCCACTTCCGG--------------------------------------------------GGACAAAGGAAGCC`	`50 bp deleted → frameshift`
31.5	`CCCTGGCCACTTCCGGCGCGCCGA---------------------------------------------CAAAGGAAGCC`	`45 bp deleted → no frameshift`
31.2	`CCCTGGCCACTTCCGG---------------------------------------------------GACAAAGGAAGCC`	`51 bp deleted → no frameshift`
30.0	`CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAG----------------------------------------------CC`	`46 bp deleted → frameshift`
29.6	`CCCTGGCCACTTCCGGCGCGCCGAGTCC----------------------------------------------------`	`52 bp deleted → frameshift`
29.6	`CCCTGGCCACTTCCGG----------------------------------------------------ACAAAGGAAGCC`	`52 bp deleted → frameshift`
25.8	`CCCTGGCCACTTCCGGCGCGCCGAG-------------------------------------------------GAAGCC`	`49 bp deleted → frameshift`
25.6	`CCC-------------------------------------------------------CAGATTGGGGACAAAGGAAGCC`	`55 bp deleted → frameshift`
25.0	`CCCTGG------------------------------------------------------------GGACAAAGGAAGCC`	`60 bp deleted → no frameshift`
23.2	`CC---------------------------------------------------------AGATTGGGGACAAAGGAAGCC`	`57 bp deleted → no frameshift`
22.2	`CCCTGGCCACTTCCGGCGCGCCGA----------------------------------------------------AGCC`	`52 bp deleted → frameshift`
21.3	`CCCTGGCCACTTCCGGCGCGCCGAG-----------------------------------------------------CC`	`53 bp deleted → frameshift`
20.8	`CCCTGGCCACTTCCGG-----------------------------------------------------------AAGCC`	`59 bp deleted → frameshift`
20.0	`CCCTGGCCACTTCCGGCGC------------------------------------------------------------C`	`60 bp deleted → no frameshift`
18.8	`CCCTGG-------------------------------------------------------------GACAAAGGAAGCC`	`61 bp deleted → frameshift`
18.0	`CCCTGGCCACTTCCGGC--------------------------------------------------------------C`	`62 bp deleted → frameshift`
18.0	`CCCTGG--------------------------------------------------------------ACAAAGGAAGCC`	`62 bp deleted → frameshift`
16.2	`CCCTGGCC------------------------------------------------------------------------`	`72 bp deleted → no frameshift`
15.0	`CCCTGGCCAC------------------------------------------------------------AAAGGAAGCC`	`60 bp deleted → no frameshift`
14.8	`CCCTGGCCACTTCC------------------------------------------------------------------`	`66 bp deleted → no frameshift`
14.8	`CCCTGGCCACTT----------------------------------------------------GGGGACAAAGGAAGCC`	`52 bp deleted → frameshift`
13.5	`CCCTGGCCA--------------------------------------------------------------AAGGAAGCC`	`62 bp deleted → frameshift`
12.8	`CCCTGG---------------------------------------------------------------------AAGCC`	`69 bp deleted → no frameshift`
8.4	`CCC-----------------------------------------------------------------------------`	`77 bp deleted → frameshift`
8.0	`CC------------------------------------------------------------------------------`	`78 bp deleted → no frameshift`

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