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Micro-homology scoring of potential deletions

Guide: CCGAGTCCTTAGGCCGCCAG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score Sequence Effect
CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC (Wild-type sequence)
485.6 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCC----------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 10 bp deleted → frameshift
417.6 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCG-------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCC 13 bp deleted → frameshift
346.2 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC-----------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 11 bp deleted → frameshift
329.4 CCCTGGCCACTTCCGGCGCGC----------------------------------GCCCAGATTGGGGACAAAGGAAGCC 34 bp deleted → frameshift
327.6 CCCTGGCCACTTCCGGCGCGCCG--------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCC 26 bp deleted → frameshift
294.4 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCC--------------------CAGATTGGGGACAAAGGAAGCC 20 bp deleted → frameshift
269.4 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC----------------GCGCCCAGATTGGGGACAAAGGAAGCC 16 bp deleted → frameshift
268.0 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC--------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 8 bp deleted → frameshift
256.2 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC-----------------GCGCGCCCAGATTGGGGACAAAGGAAGCC 17 bp deleted → frameshift
255.2 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG---------GGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 9 bp deleted → no frameshift
247.0 CCCTGGCCACTTCCGGCGC----------------------------CGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 28 bp deleted → frameshift
245.0 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG---------------------ATTGGGGACAAAGGAAGCC 21 bp deleted → no frameshift
244.2 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC------------------GCCCAGATTGGGGACAAAGGAAGCC 18 bp deleted → no frameshift
242.8 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG----------GCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 10 bp deleted → frameshift
231.0 CCCTGGCCACTTCCGGCGCGCC------------------------------------CAGATTGGGGACAAAGGAAGCC 36 bp deleted → no frameshift
198.8 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC--------------GCGCGCCCAGATTGGGGACAAAGGAAGCC 14 bp deleted → frameshift
190.2 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCC-----------------------CAGATTGGGGACAAAGGAAGCC 23 bp deleted → frameshift
161.6 CCCTGGCCACTTCCGGCGCGC--------------------------------GCGCCCAGATTGGGGACAAAGGAAGCC 32 bp deleted → frameshift
154.8 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC-------------------GCGCCCAGATTGGGGACAAAGGAAGCC 19 bp deleted → frameshift
147.2 CCCTGGCCACTTCCGGCGCGCCGAGTCC--------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 20 bp deleted → frameshift
140.0 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC---------------------GCCCAGATTGGGGACAAAGGAAGCC 21 bp deleted → no frameshift
126.8 CCCTGGCCACTTCCGGCGCGCCG-----------------------CCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 23 bp deleted → frameshift
120.4 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCC------------------------AGATTGGGGACAAAGGAAGCC 24 bp deleted → no frameshift
120.4 CCCTGGCCACTTCCGGCGCGC------------------------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 24 bp deleted → no frameshift
120.0 CCCTGGCCACTTCCGGCGC--------------------------------------CCAGATTGGGGACAAAGGAAGCC 38 bp deleted → frameshift
109.2 CCCTGGCCACTTCCGG--------------------------GGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 26 bp deleted → frameshift
103.6 CCCTGGCCACTTCCGG---------------------------GCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 27 bp deleted → no frameshift
94.0 CCCTGGCCACTTCCGGCGCGCCG-----------------------------CGCGCCCAGATTGGGGACAAAGGAAGCC 29 bp deleted → frameshift
94.0 CCCTGGCCACTTCCGGCGCG-----------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCC 29 bp deleted → frameshift
90.0 CCCTGGC--------------------------------------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 38 bp deleted → frameshift
89.2 CCCTGGCCACTTCCGGCGCGCCGAGTCC------------------------------CAGATTGGGGACAAAGGAAGCC 30 bp deleted → no frameshift
89.2 CCCTGGCCACTTCCGGCGCGC------------------------------GCGCGCCCAGATTGGGGACAAAGGAAGCC 30 bp deleted → no frameshift
89.2 CCCTGGCCACTTCCGGC------------------------------CGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 30 bp deleted → no frameshift
84.8 CCCTGGCCACTTCCGGCGCGCCGAGTCC-------------------------------AGATTGGGGACAAAGGAAGCC 31 bp deleted → frameshift
84.8 CCCTGGCCACTTCCGGCGCGCCG-------------------------------CGCCCAGATTGGGGACAAAGGAAGCC 31 bp deleted → frameshift
84.8 CCCTGGCCACTTCCGGCG-------------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCC 31 bp deleted → frameshift
84.8 CCCTGGCCACTTCCG-------------------------------CCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 31 bp deleted → frameshift
81.0 CCCTGGCC----------------------------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 40 bp deleted → frameshift
76.8 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG---------------------------------GGACAAAGGAAGCC 33 bp deleted → no frameshift
76.8 CCCTGGCCACTTCCGGCGCGCCG---------------------------------CCCAGATTGGGGACAAAGGAAGCC 33 bp deleted → no frameshift
73.2 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG----------------------------------GACAAAGGAAGCC 34 bp deleted → frameshift
73.2 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCC------------------------------------------ 42 bp deleted → no frameshift
70.5 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAG-----------------------------ATTGGGGACAAAGGAAGCC 29 bp deleted → frameshift
69.6 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG-----------------------------------ACAAAGGAAGCC 35 bp deleted → frameshift
66.6 CCCTGGC--------------------------------------------GCGCGCCCAGATTGGGGACAAAGGAAGCC 44 bp deleted → frameshift
66.0 CCCTGG------------------------------------GGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 36 bp deleted → no frameshift
63.0 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCC--------------------------------------------- 45 bp deleted → no frameshift
62.8 CCCTGGCCACTTCCGGCGCGCC-------------------------------------AGATTGGGGACAAAGGAAGCC 37 bp deleted → frameshift
62.8 CCCTGGCCACTTCCG-------------------------------------CGCGCCCAGATTGGGGACAAAGGAAGCC 37 bp deleted → frameshift
62.8 CCCTGG-------------------------------------GCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 37 bp deleted → frameshift
61.0 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG------------------------------------------AAGCC 42 bp deleted → no frameshift
60.6 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCA--------------------------------AAGGAAGCC 32 bp deleted → frameshift
56.8 CCCTGGCCACTTCCG---------------------------------------CGCCCAGATTGGGGACAAAGGAAGCC 39 bp deleted → no frameshift
54.9 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG----------------------------------GAAGCC 34 bp deleted → frameshift
54.0 CCCTGGCCACTTCCGGC----------------------------------------CCAGATTGGGGACAAAGGAAGCC 40 bp deleted → frameshift
51.6 CCCTGGCCACTTCCG-----------------------------------------CCCAGATTGGGGACAAAGGAAGCC 41 bp deleted → frameshift
49.5 CCCTGGCCACTTCCGGCGCGCCGAG------------------------------------ATTGGGGACAAAGGAAGCC 36 bp deleted → no frameshift
49.2 CCCTGGCC--------------------------------------------------CAGATTGGGGACAAAGGAAGCC 50 bp deleted → frameshift
45.0 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG--------------------------------------CC 38 bp deleted → frameshift
45.0 CCCTGGCCACTTCCGGCGCGCCGA--------------------------------------TTGGGGACAAAGGAAGCC 38 bp deleted → frameshift
44.4 CCCTGGCCACTTCC--------------------------------------------CAGATTGGGGACAAAGGAAGCC 44 bp deleted → frameshift
42.0 CCCTGGCCACTTCC---------------------------------------------AGATTGGGGACAAAGGAAGCC 45 bp deleted → no frameshift
42.0 CCC---------------------------------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 45 bp deleted → no frameshift
40.0 CCCTGGC----------------------------------------------GCGCCCAGATTGGGGACAAAGGAAGCC 46 bp deleted → frameshift
40.0 CC----------------------------------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC 46 bp deleted → frameshift
39.0 CCCTGGCCA---------------------------------------------------GATTGGGGACAAAGGAAGCC 51 bp deleted → no frameshift
36.6 CCCTGGCCACTTCCGGCGCGCCGAGTCCTT----------------------------------GGGGACAAAGGAAGCC 34 bp deleted → frameshift
36.6 CCC--------------------------------------------------------AGATTGGGGACAAAGGAAGCC 56 bp deleted → frameshift
36.4 CCCTGGC------------------------------------------------GCCCAGATTGGGGACAAAGGAAGCC 48 bp deleted → no frameshift
33.0 CCCTGGCCACTTCCGGCGCGCC---------------------------------------------------------- 58 bp deleted → frameshift
32.8 CCCTGGCCACTTCCGG--------------------------------------------------GGACAAAGGAAGCC 50 bp deleted → frameshift
31.5 CCCTGGCCACTTCCGGCGCGCCGA---------------------------------------------CAAAGGAAGCC 45 bp deleted → no frameshift
31.2 CCCTGGCCACTTCCGG---------------------------------------------------GACAAAGGAAGCC 51 bp deleted → no frameshift
30.0 CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAG----------------------------------------------CC 46 bp deleted → frameshift
29.6 CCCTGGCCACTTCCGGCGCGCCGAGTCC---------------------------------------------------- 52 bp deleted → frameshift
29.6 CCCTGGCCACTTCCGG----------------------------------------------------ACAAAGGAAGCC 52 bp deleted → frameshift
25.8 CCCTGGCCACTTCCGGCGCGCCGAG-------------------------------------------------GAAGCC 49 bp deleted → frameshift
25.6 CCC-------------------------------------------------------CAGATTGGGGACAAAGGAAGCC 55 bp deleted → frameshift
25.0 CCCTGG------------------------------------------------------------GGACAAAGGAAGCC 60 bp deleted → no frameshift
23.2 CC---------------------------------------------------------AGATTGGGGACAAAGGAAGCC 57 bp deleted → no frameshift
22.2 CCCTGGCCACTTCCGGCGCGCCGA----------------------------------------------------AGCC 52 bp deleted → frameshift
21.3 CCCTGGCCACTTCCGGCGCGCCGAG-----------------------------------------------------CC 53 bp deleted → frameshift
20.8 CCCTGGCCACTTCCGG-----------------------------------------------------------AAGCC 59 bp deleted → frameshift
20.0 CCCTGGCCACTTCCGGCGC------------------------------------------------------------C 60 bp deleted → no frameshift
18.8 CCCTGG-------------------------------------------------------------GACAAAGGAAGCC 61 bp deleted → frameshift
18.0 CCCTGGCCACTTCCGGC--------------------------------------------------------------C 62 bp deleted → frameshift
18.0 CCCTGG--------------------------------------------------------------ACAAAGGAAGCC 62 bp deleted → frameshift
16.2 CCCTGGCC------------------------------------------------------------------------ 72 bp deleted → no frameshift
15.0 CCCTGGCCAC------------------------------------------------------------AAAGGAAGCC 60 bp deleted → no frameshift
14.8 CCCTGGCCACTTCC------------------------------------------------------------------ 66 bp deleted → no frameshift
14.8 CCCTGGCCACTT----------------------------------------------------GGGGACAAAGGAAGCC 52 bp deleted → frameshift
13.5 CCCTGGCCA--------------------------------------------------------------AAGGAAGCC 62 bp deleted → frameshift
12.8 CCCTGG---------------------------------------------------------------------AAGCC 69 bp deleted → no frameshift
8.4 CCC----------------------------------------------------------------------------- 77 bp deleted → frameshift
8.0 CC------------------------------------------------------------------------------ 78 bp deleted → no frameshift
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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.