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The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
Bae-Score | Sequence | Effect |
---|---|---|
CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | (Wild-type sequence) | |
485.6 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCC----------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 10 bp deleted → frameshift |
417.6 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCG-------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 13 bp deleted → frameshift |
346.2 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC-----------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 11 bp deleted → frameshift |
329.4 | CCCTGGCCACTTCCGGCGCGC----------------------------------GCCCAGATTGGGGACAAAGGAAGCC | 34 bp deleted → frameshift |
327.6 | CCCTGGCCACTTCCGGCGCGCCG--------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 26 bp deleted → frameshift |
294.4 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCC--------------------CAGATTGGGGACAAAGGAAGCC | 20 bp deleted → frameshift |
269.4 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC----------------GCGCCCAGATTGGGGACAAAGGAAGCC | 16 bp deleted → frameshift |
268.0 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC--------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 8 bp deleted → frameshift |
256.2 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC-----------------GCGCGCCCAGATTGGGGACAAAGGAAGCC | 17 bp deleted → frameshift |
255.2 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG---------GGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 9 bp deleted → no frameshift |
247.0 | CCCTGGCCACTTCCGGCGC----------------------------CGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 28 bp deleted → frameshift |
245.0 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG---------------------ATTGGGGACAAAGGAAGCC | 21 bp deleted → no frameshift |
244.2 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC------------------GCCCAGATTGGGGACAAAGGAAGCC | 18 bp deleted → no frameshift |
242.8 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG----------GCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 10 bp deleted → frameshift |
231.0 | CCCTGGCCACTTCCGGCGCGCC------------------------------------CAGATTGGGGACAAAGGAAGCC | 36 bp deleted → no frameshift |
198.8 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGC--------------GCGCGCCCAGATTGGGGACAAAGGAAGCC | 14 bp deleted → frameshift |
190.2 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCC-----------------------CAGATTGGGGACAAAGGAAGCC | 23 bp deleted → frameshift |
161.6 | CCCTGGCCACTTCCGGCGCGC--------------------------------GCGCCCAGATTGGGGACAAAGGAAGCC | 32 bp deleted → frameshift |
154.8 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC-------------------GCGCCCAGATTGGGGACAAAGGAAGCC | 19 bp deleted → frameshift |
147.2 | CCCTGGCCACTTCCGGCGCGCCGAGTCC--------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 20 bp deleted → frameshift |
140.0 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGC---------------------GCCCAGATTGGGGACAAAGGAAGCC | 21 bp deleted → no frameshift |
126.8 | CCCTGGCCACTTCCGGCGCGCCG-----------------------CCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 23 bp deleted → frameshift |
120.4 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCC------------------------AGATTGGGGACAAAGGAAGCC | 24 bp deleted → no frameshift |
120.4 | CCCTGGCCACTTCCGGCGCGC------------------------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 24 bp deleted → no frameshift |
120.0 | CCCTGGCCACTTCCGGCGC--------------------------------------CCAGATTGGGGACAAAGGAAGCC | 38 bp deleted → frameshift |
109.2 | CCCTGGCCACTTCCGG--------------------------GGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 26 bp deleted → frameshift |
103.6 | CCCTGGCCACTTCCGG---------------------------GCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 27 bp deleted → no frameshift |
94.0 | CCCTGGCCACTTCCGGCGCGCCG-----------------------------CGCGCCCAGATTGGGGACAAAGGAAGCC | 29 bp deleted → frameshift |
94.0 | CCCTGGCCACTTCCGGCGCG-----------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 29 bp deleted → frameshift |
90.0 | CCCTGGC--------------------------------------GCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 38 bp deleted → frameshift |
89.2 | CCCTGGCCACTTCCGGCGCGCCGAGTCC------------------------------CAGATTGGGGACAAAGGAAGCC | 30 bp deleted → no frameshift |
89.2 | CCCTGGCCACTTCCGGCGCGC------------------------------GCGCGCCCAGATTGGGGACAAAGGAAGCC | 30 bp deleted → no frameshift |
89.2 | CCCTGGCCACTTCCGGC------------------------------CGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 30 bp deleted → no frameshift |
84.8 | CCCTGGCCACTTCCGGCGCGCCGAGTCC-------------------------------AGATTGGGGACAAAGGAAGCC | 31 bp deleted → frameshift |
84.8 | CCCTGGCCACTTCCGGCGCGCCG-------------------------------CGCCCAGATTGGGGACAAAGGAAGCC | 31 bp deleted → frameshift |
84.8 | CCCTGGCCACTTCCGGCG-------------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 31 bp deleted → frameshift |
84.8 | CCCTGGCCACTTCCG-------------------------------CCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 31 bp deleted → frameshift |
81.0 | CCCTGGCC----------------------------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 40 bp deleted → frameshift |
76.8 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG---------------------------------GGACAAAGGAAGCC | 33 bp deleted → no frameshift |
76.8 | CCCTGGCCACTTCCGGCGCGCCG---------------------------------CCCAGATTGGGGACAAAGGAAGCC | 33 bp deleted → no frameshift |
73.2 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG----------------------------------GACAAAGGAAGCC | 34 bp deleted → frameshift |
73.2 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCC------------------------------------------ | 42 bp deleted → no frameshift |
70.5 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAG-----------------------------ATTGGGGACAAAGGAAGCC | 29 bp deleted → frameshift |
69.6 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG-----------------------------------ACAAAGGAAGCC | 35 bp deleted → frameshift |
66.6 | CCCTGGC--------------------------------------------GCGCGCCCAGATTGGGGACAAAGGAAGCC | 44 bp deleted → frameshift |
66.0 | CCCTGG------------------------------------GGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 36 bp deleted → no frameshift |
63.0 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCC--------------------------------------------- | 45 bp deleted → no frameshift |
62.8 | CCCTGGCCACTTCCGGCGCGCC-------------------------------------AGATTGGGGACAAAGGAAGCC | 37 bp deleted → frameshift |
62.8 | CCCTGGCCACTTCCG-------------------------------------CGCGCCCAGATTGGGGACAAAGGAAGCC | 37 bp deleted → frameshift |
62.8 | CCCTGG-------------------------------------GCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 37 bp deleted → frameshift |
61.0 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGG------------------------------------------AAGCC | 42 bp deleted → no frameshift |
60.6 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCA--------------------------------AAGGAAGCC | 32 bp deleted → frameshift |
56.8 | CCCTGGCCACTTCCG---------------------------------------CGCCCAGATTGGGGACAAAGGAAGCC | 39 bp deleted → no frameshift |
54.9 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG----------------------------------GAAGCC | 34 bp deleted → frameshift |
54.0 | CCCTGGCCACTTCCGGC----------------------------------------CCAGATTGGGGACAAAGGAAGCC | 40 bp deleted → frameshift |
51.6 | CCCTGGCCACTTCCG-----------------------------------------CCCAGATTGGGGACAAAGGAAGCC | 41 bp deleted → frameshift |
49.5 | CCCTGGCCACTTCCGGCGCGCCGAG------------------------------------ATTGGGGACAAAGGAAGCC | 36 bp deleted → no frameshift |
49.2 | CCCTGGCC--------------------------------------------------CAGATTGGGGACAAAGGAAGCC | 50 bp deleted → frameshift |
45.0 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG--------------------------------------CC | 38 bp deleted → frameshift |
45.0 | CCCTGGCCACTTCCGGCGCGCCGA--------------------------------------TTGGGGACAAAGGAAGCC | 38 bp deleted → frameshift |
44.4 | CCCTGGCCACTTCC--------------------------------------------CAGATTGGGGACAAAGGAAGCC | 44 bp deleted → frameshift |
42.0 | CCCTGGCCACTTCC---------------------------------------------AGATTGGGGACAAAGGAAGCC | 45 bp deleted → no frameshift |
42.0 | CCC---------------------------------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 45 bp deleted → no frameshift |
40.0 | CCCTGGC----------------------------------------------GCGCCCAGATTGGGGACAAAGGAAGCC | 46 bp deleted → frameshift |
40.0 | CC----------------------------------------------GGCGCGCGCCCAGATTGGGGACAAAGGAAGCC | 46 bp deleted → frameshift |
39.0 | CCCTGGCCA---------------------------------------------------GATTGGGGACAAAGGAAGCC | 51 bp deleted → no frameshift |
36.6 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTT----------------------------------GGGGACAAAGGAAGCC | 34 bp deleted → frameshift |
36.6 | CCC--------------------------------------------------------AGATTGGGGACAAAGGAAGCC | 56 bp deleted → frameshift |
36.4 | CCCTGGC------------------------------------------------GCCCAGATTGGGGACAAAGGAAGCC | 48 bp deleted → no frameshift |
33.0 | CCCTGGCCACTTCCGGCGCGCC---------------------------------------------------------- | 58 bp deleted → frameshift |
32.8 | CCCTGGCCACTTCCGG--------------------------------------------------GGACAAAGGAAGCC | 50 bp deleted → frameshift |
31.5 | CCCTGGCCACTTCCGGCGCGCCGA---------------------------------------------CAAAGGAAGCC | 45 bp deleted → no frameshift |
31.2 | CCCTGGCCACTTCCGG---------------------------------------------------GACAAAGGAAGCC | 51 bp deleted → no frameshift |
30.0 | CCCTGGCCACTTCCGGCGCGCCGAGTCCTTAG----------------------------------------------CC | 46 bp deleted → frameshift |
29.6 | CCCTGGCCACTTCCGGCGCGCCGAGTCC---------------------------------------------------- | 52 bp deleted → frameshift |
29.6 | CCCTGGCCACTTCCGG----------------------------------------------------ACAAAGGAAGCC | 52 bp deleted → frameshift |
25.8 | CCCTGGCCACTTCCGGCGCGCCGAG-------------------------------------------------GAAGCC | 49 bp deleted → frameshift |
25.6 | CCC-------------------------------------------------------CAGATTGGGGACAAAGGAAGCC | 55 bp deleted → frameshift |
25.0 | CCCTGG------------------------------------------------------------GGACAAAGGAAGCC | 60 bp deleted → no frameshift |
23.2 | CC---------------------------------------------------------AGATTGGGGACAAAGGAAGCC | 57 bp deleted → no frameshift |
22.2 | CCCTGGCCACTTCCGGCGCGCCGA----------------------------------------------------AGCC | 52 bp deleted → frameshift |
21.3 | CCCTGGCCACTTCCGGCGCGCCGAG-----------------------------------------------------CC | 53 bp deleted → frameshift |
20.8 | CCCTGGCCACTTCCGG-----------------------------------------------------------AAGCC | 59 bp deleted → frameshift |
20.0 | CCCTGGCCACTTCCGGCGC------------------------------------------------------------C | 60 bp deleted → no frameshift |
18.8 | CCCTGG-------------------------------------------------------------GACAAAGGAAGCC | 61 bp deleted → frameshift |
18.0 | CCCTGGCCACTTCCGGC--------------------------------------------------------------C | 62 bp deleted → frameshift |
18.0 | CCCTGG--------------------------------------------------------------ACAAAGGAAGCC | 62 bp deleted → frameshift |
16.2 | CCCTGGCC------------------------------------------------------------------------ | 72 bp deleted → no frameshift |
15.0 | CCCTGGCCAC------------------------------------------------------------AAAGGAAGCC | 60 bp deleted → no frameshift |
14.8 | CCCTGGCCACTTCC------------------------------------------------------------------ | 66 bp deleted → no frameshift |
14.8 | CCCTGGCCACTT----------------------------------------------------GGGGACAAAGGAAGCC | 52 bp deleted → frameshift |
13.5 | CCCTGGCCA--------------------------------------------------------------AAGGAAGCC | 62 bp deleted → frameshift |
12.8 | CCCTGG---------------------------------------------------------------------AAGCC | 69 bp deleted → no frameshift |
8.4 | CCC----------------------------------------------------------------------------- | 77 bp deleted → frameshift |
8.0 | CC------------------------------------------------------------------------------ | 78 bp deleted → no frameshift |