CRISPOR

Micro-homology scoring of potential deletions

Guide: CGGCGCGCCGGAAGTGGCCA GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	(Wild-type sequence)
444.6	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC------GGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`6 bp deleted → no frameshift`
329.4	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC------------GACCTCGGCTCACAGCGCGCCCGGCTATTC`	`12 bp deleted → no frameshift`
311.6	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGG-----GCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`5 bp deleted → frameshift`
294.4	`CCCCTGGCGGCCTAAGGACTCGGCG--------------------GGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`20 bp deleted → frameshift`
283.2	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGG---------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`15 bp deleted → no frameshift`
255.2	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGG---------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`9 bp deleted → no frameshift`
244.2	`CCCCTGGCGGCCTAAGGACTCGGCGCG------------------GGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`18 bp deleted → no frameshift`
242.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGG----------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`10 bp deleted → frameshift`
230.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGG-----------GCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`11 bp deleted → frameshift`
230.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGG-----------GCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`11 bp deleted → frameshift`
219.6	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGG------------CGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`12 bp deleted → no frameshift`
218.4	`CCCCTGGCGGCCTAAGGACTCGGCG--------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTC`	`26 bp deleted → frameshift`
210.0	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC---------------------TCACAGCGCGCCCGGCTATTC`	`21 bp deleted → no frameshift`
191.4	`CCCCTGGCGGCCTAAGGACTCGGC-----------------------------------TCACAGCGCGCCCGGCTATTC`	`35 bp deleted → frameshift`
190.2	`CCCCTGGCGGCCTAAGGACTCGG-----------------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`23 bp deleted → frameshift`
188.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC---------------TCGGCTCACAGCGCGCCCGGCTATTC`	`15 bp deleted → no frameshift`
180.6	`CCCCTGGCGGCCTAAGGACTCGGCGCG------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTC`	`24 bp deleted → no frameshift`
179.6	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGG----------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`16 bp deleted → frameshift`
179.6	`CCCCTGGCGGCCTAAGGACTCGGCGCGC----------------GGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`16 bp deleted → frameshift`
170.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGG-----------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`17 bp deleted → frameshift`
170.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCC------------------------------------------CGGCTATTC`	`42 bp deleted → no frameshift`
165.0	`CCCCTGGCGG------------------------------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`36 bp deleted → no frameshift`
162.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGG------------------CGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`18 bp deleted → no frameshift`
155.4	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGG---------------------------CTCACAGCGCGCCCGGCTATTC`	`27 bp deleted → no frameshift`
154.8	`CCCCTGGCGGCCTAAGGACTCGG-------------------GCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`19 bp deleted → frameshift`
140.0	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCG---------------------ACCTCGGCTCACAGCGCGCCCGGCTATTC`	`21 bp deleted → no frameshift`
133.2	`CCCCTGGCGGCCTAAGGACTCGGCGCGC----------------------GACCTCGGCTCACAGCGCGCCCGGCTATTC`	`22 bp deleted → frameshift`
121.2	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC--------------------------------CGGCTATTC`	`32 bp deleted → frameshift`
120.4	`CCCCTGGCGGCCTAAGGACTCGG------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`24 bp deleted → no frameshift`
115.2	`CCCCTGGCGGC---------------------------------GGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`33 bp deleted → no frameshift`
114.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCC-------------------------TCGGCTCACAGCGCGCCCGGCTATTC`	`25 bp deleted → frameshift`
114.8	`CCCCTGGCGGCCTAAGGACTCGG-------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`25 bp deleted → frameshift`
114.8	`CCCCTGGCGGCCTAAGG-------------------------GCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`25 bp deleted → frameshift`
110.4	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGA--------------------CCTCGGCTCACAGCGCGCCCGGCTATTC`	`20 bp deleted → frameshift`
109.2	`CCCCTGGCGGCCTAAGG--------------------------CGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`26 bp deleted → frameshift`
108.0	`CCCCTGGCGGCCTAAGGACTCGGCGCGC----------------------------------------GCCCGGCTATTC`	`40 bp deleted → frameshift`
99.9	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCA----------------------CAGCGCGCCCGGCTATTC`	`22 bp deleted → frameshift`
98.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC----------------------------GCGCCCGGCTATTC`	`28 bp deleted → frameshift`
97.6	`CCCCTGGCG------------------------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTC`	`42 bp deleted → no frameshift`
94.2	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC-------------------------------------TATTC`	`37 bp deleted → frameshift`
94.0	`CCCCTGGCGGCCTAAGGACTCG-----------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTC`	`29 bp deleted → frameshift`
94.0	`CCCCTGGCGGCCTAAGG-----------------------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`29 bp deleted → frameshift`
92.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGG-------------------------------------------CTATTC`	`43 bp deleted → frameshift`
91.5	`CCCCTGGCGGCCTAAGGAC----------------------------------CTCGGCTCACAGCGCGCCCGGCTATTC`	`34 bp deleted → frameshift`
90.3	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCA------------------------GCGCGCCCGGCTATTC`	`24 bp deleted → no frameshift`
89.2	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC------------------------------GCCCGGCTATTC`	`30 bp deleted → no frameshift`
89.2	`CCCCTGGCGGCCTAAGGACTCGGCGCG------------------------------GCTCACAGCGCGCCCGGCTATTC`	`30 bp deleted → no frameshift`
89.2	`CCCCTGGCGGCCTAAGG------------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`30 bp deleted → no frameshift`
88.8	`CCCCTGGCGGCCTAAGGACTCGGCGC--------------------------------------------CCGGCTATTC`	`44 bp deleted → frameshift`
85.2	`CCCCTGGCGGC---------------------------------------GACCTCGGCTCACAGCGCGCCCGGCTATTC`	`39 bp deleted → no frameshift`
84.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGC-------------------------------TCACAGCGCGCCCGGCTATTC`	`31 bp deleted → frameshift`
84.8	`CCCCTGGCGGCCTAAGG-------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`31 bp deleted → frameshift`
80.8	`CCCCTGGCGGCCTAAGGACTCGGCG--------------------------------GCTCACAGCGCGCCCGGCTATTC`	`32 bp deleted → frameshift`
80.8	`CCCCTGGCGGCCTAAGG--------------------------------CGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`32 bp deleted → frameshift`
80.8	`CCCCTGGCGG--------------------------------GCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`32 bp deleted → frameshift`
76.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC---------------------------------GGCTATTC`	`33 bp deleted → no frameshift`
76.8	`CCCCTGGCGGCCTAAGGACTCGGCGC---------------------------------TCACAGCGCGCCCGGCTATTC`	`33 bp deleted → no frameshift`
72.8	`CCCCTGGCGGC------------------------------------------------TCACAGCGCGCCCGGCTATTC`	`48 bp deleted → no frameshift`
69.6	`CCCCTGG-----------------------------------GCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`35 bp deleted → frameshift`
67.5	`CCCCTGGCGGCCTAAGGACTC----------------------------------------ACAGCGCGCCCGGCTATTC`	`40 bp deleted → frameshift`
63.6	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG-------------------------------CGCGCCCGGCTATTC`	`31 bp deleted → frameshift`
62.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCG-------------------------------------CGCCCGGCTATTC`	`37 bp deleted → frameshift`
62.8	`CCCCTGGCGG-------------------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`37 bp deleted → frameshift`
62.4	`CCCCTGGCGGCCTAAGGACTCGGC---------------------------------------------------TATTC`	`51 bp deleted → no frameshift`
61.0	`CCCCTGGCGGCCT------------------------------------------CGGCTCACAGCGCGCCCGGCTATTC`	`42 bp deleted → no frameshift`
60.0	`CCCCTGGCGGCCTAAGGACTCGGCGCGC--------------------------------------GCGCCCGGCTATTC`	`38 bp deleted → frameshift`
60.0	`CCCCTGGCGG--------------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`38 bp deleted → frameshift`
56.8	`CCCCTGGCGGCCTAAGGACTCGGCGCGCCG---------------------------------------CCCGGCTATTC`	`39 bp deleted → no frameshift`
56.8	`CCCCTGG---------------------------------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`39 bp deleted → no frameshift`
54.0	`CCCCTGG----------------------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`40 bp deleted → frameshift`
51.6	`CCCCTGGCGGCCTAAGG-----------------------------------------CTCACAGCGCGCCCGGCTATTC`	`41 bp deleted → frameshift`
51.6	`CCCCTGG-----------------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTC`	`41 bp deleted → frameshift`
46.8	`CCCCTGGC---------------------------------------------------TCACAGCGCGCCCGGCTATTC`	`51 bp deleted → no frameshift`
42.0	`CCCCTGGCGGCCTAAGGACTCG---------------------------------------------CGCCCGGCTATTC`	`45 bp deleted → no frameshift`
41.0	`CCCCT--------------------------------------------------CGGCTCACAGCGCGCCCGGCTATTC`	`50 bp deleted → frameshift`
40.0	`CCCCTGGCGGCCTAAGGACTCGGCGCG----------------------------------------------GCTATTC`	`46 bp deleted → frameshift`
40.0	`CCCCTGGCGGCCTAAGGACTCGGC----------------------------------------------CCGGCTATTC`	`46 bp deleted → frameshift`
38.0	`CCCCTGGCGGCCTAAGGACTCGGCGCGC-----------------------------------------------TATTC`	`47 bp deleted → frameshift`
38.0	`CCCCTGGCGGCCTAAGGACTCG-----------------------------------------------CCCGGCTATTC`	`47 bp deleted → frameshift`
36.4	`CCCCTGGCGGCCTAAGGACTCGGCG------------------------------------------------GCTATTC`	`48 bp deleted → no frameshift`
34.4	`CCCCTGGCGGCCTAAGGACTCGGCGC-------------------------------------------------TATTC`	`49 bp deleted → frameshift`
33.3	`CCCCTGGCGGCCTAAGGAC--------------------------------------------AGCGCGCCCGGCTATTC`	`44 bp deleted → frameshift`
33.0	`CCCCTGGCG----------------------------------------------------------CGCCCGGCTATTC`	`58 bp deleted → frameshift`
32.8	`CCCCTGGCGGC----------------------------------------------------------------TATTC`	`64 bp deleted → frameshift`
31.2	`CCCCTGGCGGCC-----------------------------------------------------------CGGCTATTC`	`59 bp deleted → frameshift`
31.2	`CCC---------------------------------------------------TCGGCTCACAGCGCGCCCGGCTATTC`	`51 bp deleted → no frameshift`
30.0	`CCCCTGGCG------------------------------------------------------------CCCGGCTATTC`	`60 bp deleted → no frameshift`
29.6	`CC----------------------------------------------------TCGGCTCACAGCGCGCCCGGCTATTC`	`52 bp deleted → frameshift`
28.5	`CCCCTGGCGGCCT-----------------------------------------------CACAGCGCGCCCGGCTATTC`	`47 bp deleted → frameshift`
25.8	`CCCCTGGCGGCCTAAG-------------------------------------------------CGCGCCCGGCTATTC`	`49 bp deleted → frameshift`
25.6	`CCCCTGGCGGC-------------------------------------------------------GCGCCCGGCTATTC`	`55 bp deleted → frameshift`
23.2	`CCCCTGGCGGCCTAAGG---------------------------------------------------------CTATTC`	`57 bp deleted → no frameshift`
23.2	`CCCCTGGCGGC---------------------------------------------------------GCCCGGCTATTC`	`57 bp deleted → no frameshift`
21.0	`CCCCTGGC-------------------------------------------------------------------TATTC`	`67 bp deleted → frameshift`
20.0	`CCCCTGGCGGCC------------------------------------------------------------GGCTATTC`	`60 bp deleted → no frameshift`
19.8	`CCCC--------------------------------------------------------------------GGCTATTC`	`68 bp deleted → frameshift`
19.2	`CCCCT-------------------------------------------------------CACAGCGCGCCCGGCTATTC`	`55 bp deleted → frameshift`
19.2	`CCC---------------------------------------------------------------------GGCTATTC`	`69 bp deleted → no frameshift`
18.3	`CCCCTGGCGGCCTAAGGACT--------------------------------------------------------ATTC`	`56 bp deleted → frameshift`
18.0	`CCCCTGGC--------------------------------------------------------------CCGGCTATTC`	`62 bp deleted → frameshift`
17.2	`CCCCTGGCGGCCTA---------------------------------------------------------------TTC`	`63 bp deleted → no frameshift`
15.6	`CCCCTGGCGGCCTAAGGACTC-----------------------------------------------------------`	`59 bp deleted → frameshift`
14.0	`CCCC-------------------------------------------------------------------CGGCTATTC`	`67 bp deleted → frameshift`
12.0	`CC----------------------------------------------------------------------GGCTATTC`	`70 bp deleted → frameshift`
8.7	`CCCCT-----------------------------------------------------------------------ATTC`	`71 bp deleted → frameshift`

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