CRISPOR

Micro-homology scoring of potential deletions

Guide: CGCGGCGATATCATCATCCA TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	(Wild-type sequence)
200.9	`GAGCCGTTGTCGACGACGAGCGCGGCGA-------------------------GAATAGCCGGGCGCGCTGTGAGCCGAG`	`25 bp deleted → frameshift`
181.3	`GAGCCGTTGTCGACGACGAGC---------------------------TGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`27 bp deleted → no frameshift`
162.8	`GAGCCGTTGTCGACGACGAGCGCGG------------------TGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`18 bp deleted → no frameshift`
148.2	`GAGCCGTTGTCGACGACGAGCGCG----------------------------AGAATAGCCGGGCGCGCTGTGAGCCGAG`	`28 bp deleted → frameshift`
133.8	`GAGCCGTTGTCGACGACGAGCG------------------------------AGAATAGCCGGGCGCGCTGTGAGCCGAG`	`30 bp deleted → no frameshift`
133.2	`GAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATCC----------------------GGGCGCGCTGTGAGCCGAG`	`22 bp deleted → frameshift`
133.2	`GAGCCGTTGTCGACGACGAGCGCGGC----------------------TGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`22 bp deleted → frameshift`
128.1	`GAGCCGTTGTCGACGACGAG----------------------------------AATAGCCGGGCGCGCTGTGAGCCGAG`	`34 bp deleted → frameshift`
122.1	`GAGCCGTTGTCGACGACGAGCGCGGCGA------------------GCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`18 bp deleted → no frameshift`
114.8	`GAGCCGTTGTCGACGACGAGCGC-------------------------TGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`25 bp deleted → frameshift`
113.6	`GAGCCGTTGTCGACGACGAGCGCGGCG---------------------------------------CGCTGTGAGCCGAG`	`39 bp deleted → no frameshift`
111.0	`GAGCCGTTGTCGACGACGAGCGCG--------------------------------------------CTGTGAGCCGAG`	`44 bp deleted → frameshift`
90.0	`GAGCCGTTGTCGACGACGAGCGCGG--------------------------------------GCGCGCTGTGAGCCGAG`	`38 bp deleted → frameshift`
80.0	`GAGCCGTTGTCGACGACGAGCGC----------------------------------------------TGTGAGCCGAG`	`46 bp deleted → frameshift`
78.5	`GAGCCGTTGTCGACGA-------------------------------------GAATAGCCGGGCGCGCTGTGAGCCGAG`	`37 bp deleted → frameshift`
77.7	`GAGCCGTTGTCGACGACGAGCGCGGCGA---------------------------ATAGCCGGGCGCGCTGTGAGCCGAG`	`27 bp deleted → no frameshift`
77.7	`GAGC--------------------------------------------TGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`44 bp deleted → frameshift`
77.4	`GAGCCGTTGTCGACGACGAGCGCGGCG-----------------------------------------CTGTGAGCCGAG`	`41 bp deleted → frameshift`
74.1	`GAGCCGTTGTCGACGACGAGCGCGGCGATA----------------------------GCCGGGCGCGCTGTGAGCCGAG`	`28 bp deleted → frameshift`
73.6	`GAGCCGTTGTCGACGACGAGCGCGGCGATATCATCAT--------------------AGCCGGGCGCGCTGTGAGCCGAG`	`20 bp deleted → frameshift`
73.2	`GAGCCGTTGTCGACGACGAGCGCGGC----------------------------------CGGGCGCGCTGTGAGCCGAG`	`34 bp deleted → frameshift`
73.2	`GAGCCGTTGTCGACGACGAGCGCG------------------------------------------CGCTGTGAGCCGAG`	`42 bp deleted → no frameshift`
71.0	`GAGCCGTTGTCGACGACGAGC---------------------------------------CGGGCGCGCTGTGAGCCGAG`	`39 bp deleted → no frameshift`
69.6	`GAGCCGTTGTCGACGACGAGCGCGGCG-----------------------------------GGCGCGCTGTGAGCCGAG`	`35 bp deleted → frameshift`
67.5	`GAGCCGTTGTCGA----------------------------------------GAATAGCCGGGCGCGCTGTGAGCCGAG`	`40 bp deleted → frameshift`
66.9	`GAGCCGTTGTCGACGA------------------------------GCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`30 bp deleted → no frameshift`
63.4	`GAGCCGTTGTCGACGACGAGCGCGGCGATATCAT-----------------------AGCCGGGCGCGCTGTGAGCCGAG`	`23 bp deleted → frameshift`
62.8	`GAGCCGTTGTCGACGACGAGCGC-------------------------------------CGGGCGCGCTGTGAGCCGAG`	`37 bp deleted → frameshift`
60.0	`GAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATCC--------------------------------------GAG`	`38 bp deleted → frameshift`
57.6	`GAGCCGTTGTCGA---------------------------------GCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`33 bp deleted → no frameshift`
57.6	`GAGCCGTTG---------------------------------GTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`33 bp deleted → no frameshift`
54.9	`GAGCCGTTGT----------------------------------GAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`34 bp deleted → frameshift`
54.9	`GAGCCG--------------------------------------------------------GGCGCGCTGTGAGCCGAG`	`56 bp deleted → frameshift`
54.6	`GAGCCGTTGTCGACGACGAGCGCGGCGATAT--------------------------AGCCGGGCGCGCTGTGAGCCGAG`	`26 bp deleted → frameshift`
54.0	`GAGCCGTTGTCGACGACGAGCG----------------------------------------GGCGCGCTGTGAGCCGAG`	`40 bp deleted → frameshift`
49.5	`GAGCCGTTGTCGACGACGA------------------------------------ATAGCCGGGCGCGCTGTGAGCCGAG`	`36 bp deleted → no frameshift`
49.5	`GAGCCGTTG------------------------------------AGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`36 bp deleted → no frameshift`
47.1	`GAGCCGT-------------------------------------GAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`37 bp deleted → frameshift`
46.4	`GAGCCGTTGTCGACGACGAGCGCGGC-------------------------------------------TGTGAGCCGAG`	`43 bp deleted → frameshift`
44.8	`GAGCCGTTGTCGACGACGAGC-------------------------------------------------------CGAG`	`55 bp deleted → frameshift`
44.4	`GAGCCGTTGTCGACGACG--------------------------------------------GGCGCGCTGTGAGCCGAG`	`44 bp deleted → frameshift`
42.6	`GAGCCGTTGTCGACGA---------------------------------------ATAGCCGGGCGCGCTGTGAGCCGAG`	`39 bp deleted → no frameshift`
40.0	`GAGCCG----------------------------------------------AGAATAGCCGGGCGCGCTGTGAGCCGAG`	`46 bp deleted → frameshift`
39.0	`GAGCCGTTGTCGACGACGAGCGCGGCGA---------------------------------------------------G`	`51 bp deleted → no frameshift`
39.0	`GAG---------------------------------------------------AATAGCCGGGCGCGCTGTGAGCCGAG`	`51 bp deleted → no frameshift`
38.7	`GAGCCGTTG-----------------------------------------CGAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`41 bp deleted → frameshift`
38.0	`GAGCCGTTGTCGACG-----------------------------------------------GGCGCGCTGTGAGCCGAG`	`47 bp deleted → frameshift`
38.0	`GAGC-----------------------------------------------GAGAATAGCCGGGCGCGCTGTGAGCCGAG`	`47 bp deleted → frameshift`
36.6	`GAGCCGTTGTCGA------------------------------------------ATAGCCGGGCGCGCTGTGAGCCGAG`	`42 bp deleted → no frameshift`
36.4	`GAGCCGTTGTCGACGACGAGC------------------------------------------------TGTGAGCCGAG`	`48 bp deleted → no frameshift`
36.4	`GAGCCGTTGTCGACGACG------------------------------------------------CGCTGTGAGCCGAG`	`48 bp deleted → no frameshift`
35.0	`GAGCCGTTGTCGACGACGAG------------------------------------------------------------`	`60 bp deleted → no frameshift`
32.8	`GAGCCGTTGTCGACGACGAGCGCGGC--------------------------------------------------CGAG`	`50 bp deleted → frameshift`
32.8	`GAGCCGTTGTCGACGACG--------------------------------------------------CTGTGAGCCGAG`	`50 bp deleted → frameshift`
32.8	`GAGCCGTTGTCG--------------------------------------------------GGCGCGCTGTGAGCCGAG`	`50 bp deleted → frameshift`
31.2	`GAGCCGTTGTCGACG---------------------------------------------------CGCTGTGAGCCGAG`	`51 bp deleted → no frameshift`
30.0	`GAGCCGTTGTCGACGACGAGCGCGGCGA----------------------------------------------GCCGAG`	`46 bp deleted → frameshift`
29.7	`GAGCCG------------------------------------------------------------------------AG`	`72 bp deleted → no frameshift`
28.4	`GAGCCGTTGTCGACGACGAGCGC-----------------------------------------------------CGAG`	`53 bp deleted → frameshift`
28.4	`GAGCCGTTGTCGACG-----------------------------------------------------CTGTGAGCCGAG`	`53 bp deleted → frameshift`
26.8	`GAGCCGTTGTCGACGACGAGCGCG------------------------------------------------------AG`	`54 bp deleted → no frameshift`
26.8	`GAGCCGTTGTCG------------------------------------------------------CGCTGTGAGCCGAG`	`54 bp deleted → no frameshift`
24.4	`GAGCCGTTGTCGACGACGAGCG--------------------------------------------------------AG`	`56 bp deleted → frameshift`
24.4	`GAGCCGTTGTCG--------------------------------------------------------CTGTGAGCCGAG`	`56 bp deleted → frameshift`
21.5	`GAGCCGTTGTCGACGA---------------------------------------------------------------G`	`63 bp deleted → no frameshift`
21.3	`GA-----------------------------------------------------ATAGCCGGGCGCGCTGTGAGCCGAG`	`53 bp deleted → frameshift`
20.0	`GAGCCG------------------------------------------------------------CGCTGTGAGCCGAG`	`60 bp deleted → no frameshift`
18.8	`GAGC-------------------------------------------------------------GCGCTGTGAGCCGAG`	`61 bp deleted → frameshift`
18.5	`GAGCCGTTGTCGA------------------------------------------------------------------G`	`66 bp deleted → no frameshift`
18.0	`GAGCCGTTGT--------------------------------------------------------------GAGCCGAG`	`62 bp deleted → frameshift`
18.0	`GAGCCG--------------------------------------------------------------CTGTGAGCCGAG`	`62 bp deleted → frameshift`
17.2	`GAGC---------------------------------------------------------------GCTGTGAGCCGAG`	`63 bp deleted → no frameshift`
16.5	`GAGCCGTTGTCGACGA----------------------------------------------------------GCCGAG`	`58 bp deleted → frameshift`
15.6	`GAGC-----------------------------------------------------------------TGTGAGCCGAG`	`65 bp deleted → frameshift`
14.1	`GAGCCGTTGTCGA-------------------------------------------------------------GCCGAG`	`61 bp deleted → frameshift`
12.3	`GAGCCGTTG----------------------------------------------------------------AGCCGAG`	`64 bp deleted → frameshift`
11.7	`GAGCCGT-----------------------------------------------------------------GAGCCGAG`	`65 bp deleted → frameshift`
10.5	`GAG-----------------------------------------------------------------------------`	`77 bp deleted → frameshift`

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