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Contents:

PCR primers for off-targets of CGATGGAGGGGAAGACGGCC CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529554_F TCGTCGGCAGCGTCCTCCCCTACCTGGTGCCT 59.9 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529554_R GTCTCGTGGGCTCGGTGGATGATGATATCGCCGCG 60.4 1.00
mm1__chr9 6.83 Mbp_chr9_6834534_F Primer3: not found at this Tm N.d. 1.00
mm1__chr9 6.83 Mbp_chr9_6834534_R Primer3: not found at this Tm N.d. 1.00
mm2__chr22 15.72 Mbp_chr22_15721566_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.93
mm2__chr22 15.72 Mbp_chr22_15721566_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.93
mm2__chr2 131.26 Mbp_chr2_131263635_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.93
mm2__chr2 131.26 Mbp_chr2_131263635_R GTCTCGTGGGCTCGGCTCTTTCTGATGCATGCCCC 58.9 0.93
mm2__chr2 130.66 Mbp_chr2_130656829_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.93
mm2__chr2 130.66 Mbp_chr2_130656829_R GTCTCGTGGGCTCGGCTCTTTCTGATGCATGCCCC 58.9 0.93
mm2__chr2 130.46 Mbp_chr2_130463840_F TCGTCGGCAGCGTCCTCTTTCTGATGCATGCCCC 58.9 0.93
mm2__chr2 130.46 Mbp_chr2_130463840_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.93
mm2__chr2 130.08 Mbp_chr2_130075268_F TCGTCGGCAGCGTCCTCTTTCTGATGCATGCCCC 58.9 0.93
mm2__chr2 130.08 Mbp_chr2_130075268_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.93
mm2__chr14 19.40 Mbp_chr14_19402805_F TCGTCGGCAGCGTCATCATGTTCTGGTGCCTGGG 60.0 0.93
mm2__chr14 19.40 Mbp_chr14_19402805_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.93
mm2__chr14 19.00 Mbp_chr14_18998947_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.93
mm2__chr14 19.00 Mbp_chr14_18998947_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.93
mm1__chr6 88.28 Mbp_chr6_88276904_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 0.84
mm1__chr6 88.28 Mbp_chr6_88276904_R GTCTCGTGGGCTCGGTGGATGATGCTATCACCGCG 60.3 0.84
mm3__chr2 131.63 Mbp_chr2_131626218_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.80
mm3__chr2 131.63 Mbp_chr2_131626218_R GTCTCGTGGGCTCGGACTCCTTCTGATGCATGCCC 60.1 0.80
mm2__chr2 73.90 Mbp_chr2_73901394_F TCGTCGGCAGCGTCAGGCTTCGCAGGAGATGATG 59.8 0.79
mm2__chr2 73.90 Mbp_chr2_73901394_R GTCTCGTGGGCTCGGTATCCAGATGAGCACGCACC 59.8 0.79
mm4__chr13 113.93 Mbp_chr13_113934998_F TCGTCGGCAGCGTCGTGAGGACGAGGTATTGGCC 60.1 0.77
mm4__chr13 113.93 Mbp_chr13_113934998_R GTCTCGTGGGCTCGGCCAGAAGAGGGAGGAATGCC 59.8 0.77
mm2__chr18 62.44 Mbp_chr18_62442088_F TCGTCGGCAGCGTCCCTCGTCATCGACAATGGCT 60.1 0.64
mm2__chr18 62.44 Mbp_chr18_62442088_R GTCTCGTGGGCTCGGGAGTCCTTCTGACCCATGCC 60.1 0.64
mm4__chr6 161.75 Mbp_chr6_161753400_F Primer3: not found at this Tm N.d. 0.63
mm4__chr6 161.75 Mbp_chr6_161753400_R Primer3: not found at this Tm N.d. 0.63
mm2__chr5 131.66 Mbp_chr5_131660013_F TCGTCGGCAGCGTCATGCCCATCATCACACCCTG 60.1 0.63
mm2__chr5 131.66 Mbp_chr5_131660013_R GTCTCGTGGGCTCGGTGTGGAAGGTCTGCTTCACG 60.2 0.63
mm4__chr5 80.30 Mbp_chr5_80302744_F TCGTCGGCAGCGTCCTCTTGCTCTGGGCCTCATC 60.1 0.60
mm4__chr5 80.30 Mbp_chr5_80302744_R GTCTCGTGGGCTCGGTGGCATATGCAAGGCTAGCT 59.5 0.60
mm4__chr3 23.45 Mbp_chr3_23450065_F TCGTCGGCAGCGTCATGGCTTTGAGGACCTCAGC 60.0 0.58
mm4__chr3 23.45 Mbp_chr3_23450065_R GTCTCGTGGGCTCGGGCAAGGGGATAAAAGCAGGC 59.5 0.58
mm4__chr13 113.94 Mbp_chr13_113936389_F TCGTCGGCAGCGTCAGTGAGGATGCGGTATTGGC 60.1 0.50
mm4__chr13 113.94 Mbp_chr13_113936389_R GTCTCGTGGGCTCGGGAAGGCCTTGGCTCCTTTGA 60.2 0.50
mm4__chr12 131.85 Mbp_chr12_131846286_F TCGTCGGCAGCGTCCATCCAGTGTCACCCTCAGC 60.3 0.47
mm4__chr12 131.85 Mbp_chr12_131846286_R GTCTCGTGGGCTCGGCAAGGCCCGGCTTCCTTATA 59.5 0.47
mm4__chr7 101.05 Mbp_chr7_101050456_F Primer3: not found at this Tm N.d. 0.41
mm4__chr7 101.05 Mbp_chr7_101050456_R Primer3: not found at this Tm N.d. 0.41
mm4__chr8 84.95 Mbp_chr8_84948668_F TCGTCGGCAGCGTCCTCTGGTATGTGCAAGGCCA 60.0 0.40
mm4__chr8 84.95 Mbp_chr8_84948668_R GTCTCGTGGGCTCGGCAGTCCTTCTGCCCTATGCC 60.1 0.40
mm4__chr1 50.15 Mbp_chr1_50152177_F Primer3: not found at this Tm N.d. 0.40
mm4__chr1 50.15 Mbp_chr1_50152177_R Primer3: not found at this Tm N.d. 0.40
mm4__chr19 49.36 Mbp_chr19_49357523_F TCGTCGGCAGCGTCAAGGAGCTCGTGAACACCAC 60.2 0.39
mm4__chr19 49.36 Mbp_chr19_49357523_R GTCTCGTGGGCTCGGTCTCACTGTGCCTGTTTGGG 60.1 0.39
mm4__chr16 88.99 Mbp_chr16_88993395_F TCGTCGGCAGCGTCCCTCACAGCCTCAGCACTTG 60.9 0.39
mm4__chr16 88.99 Mbp_chr16_88993395_R GTCTCGTGGGCTCGGGGAACCCTCATGTGAACCCC 60.3 0.39
mm4__chr16 89.59 Mbp_chr16_89588865_F TCGTCGGCAGCGTCCCAGGACTATGACAGGTGCG 60.1 0.39
mm4__chr16 89.59 Mbp_chr16_89588865_R GTCTCGTGGGCTCGGGGTGCACCTGTCATAGCTGA 59.7 0.39
mm3__chr7 75.99 Mbp_chr7_75988752_F Primer3: not found at this Tm N.d. 0.39
mm3__chr7 75.99 Mbp_chr7_75988752_R Primer3: not found at this Tm N.d. 0.39
mm4__chr9 137.27 Mbp_chr9_137271780_F Primer3: not found at this Tm N.d. 0.38
mm4__chr9 137.27 Mbp_chr9_137271780_R Primer3: not found at this Tm N.d. 0.38
mm4__chr1 214.04 Mbp_chr1_214043658_F TCGTCGGCAGCGTCAGGCTGTATGGTCTGTCAAGC 60.0 0.38
mm4__chr1 214.04 Mbp_chr1_214043658_R GTCTCGTGGGCTCGGGTGCCTCCTCCTGTGAGTTC 60.0 0.38
mm4__chr2 241.32 Mbp_chr2_241315790_F TCGTCGGCAGCGTCCAACTGCCGTGGGAGGAG 60.0 0.38
mm4__chr2 241.32 Mbp_chr2_241315790_R GTCTCGTGGGCTCGGAACTGCTTCCTGGCTCAGTG 60.2 0.38
mm4__chr21 32.10 Mbp_chr21_32104119_F Primer3: not found at this Tm N.d. 0.36
mm4__chr21 32.10 Mbp_chr21_32104119_R Primer3: not found at this Tm N.d. 0.36
mm4__chr17 31.59 Mbp_chr17_31586522_F Primer3: not found at this Tm N.d. 0.36
mm4__chr17 31.59 Mbp_chr17_31586522_R Primer3: not found at this Tm N.d. 0.36
mm4__chr15 80.60 Mbp_chr15_80596402_F TCGTCGGCAGCGTCAAAGCCGTCATGGGGTCAAT 59.9 0.34
mm4__chr15 80.60 Mbp_chr15_80596402_R GTCTCGTGGGCTCGGTCCTTGGTGAAAACCCCTGG 59.8 0.34
mm4__chr1 201.99 Mbp_chr1_201988980_F TCGTCGGCAGCGTCGAAGCCCTTCGTGTACACCC 60.6 0.32
mm4__chr1 201.99 Mbp_chr1_201988980_R GTCTCGTGGGCTCGGGGCAGGTGCACCTAACCTTA 59.6 0.32
mm1__chr19 9.52 Mbp_chr19_9518229_F TCGTCGGCAGCGTCAGTTAACAATGGCTCCGGCA 59.9 0.30
mm1__chr19 9.52 Mbp_chr19_9518229_R GTCTCGTGGGCTCGGGGAGTCCTTCTGACCGATGC 60.1 0.30
mm4__chr1 215.08 Mbp_chr1_215081839_F TCGTCGGCAGCGTCTCGCCTATTACGACGACAGC 59.9 0.30
mm4__chr1 215.08 Mbp_chr1_215081839_R GTCTCGTGGGCTCGGCGTAGGGACAGCCAAACTCG 60.7 0.30
mm4__chr11 75.27 Mbp_chr11_75269534_F TCGTCGGCAGCGTCGTCAGGTTTAGGATGGCCCC 60.1 0.30
mm4__chr11 75.27 Mbp_chr11_75269534_R GTCTCGTGGGCTCGGAAGCCCCATCAGAAGCACTG 60.3 0.30
mm4__chr18 48.23 Mbp_chr18_48229011_F TCGTCGGCAGCGTCCAGAGCCTCGAACCATCTGG 60.1 0.29
mm4__chr18 48.23 Mbp_chr18_48229011_R GTCTCGTGGGCTCGGGACTGGGATCTGGCACTGTC 60.1 0.29
mm4__chr2 115.16 Mbp_chr2_115162316_F TCGTCGGCAGCGTCGGGCAGAGAGGTAAAGGCTG 60.1 0.29
mm4__chr2 115.16 Mbp_chr2_115162316_R GTCTCGTGGGCTCGGGAACCAAGAGGCCCCATTCC 60.6 0.29
mm4__chr15 67.82 Mbp_chr15_67820632_F Primer3: not found at this Tm N.d. 0.28
mm4__chr15 67.82 Mbp_chr15_67820632_R Primer3: not found at this Tm N.d. 0.28
mm4__chr5 57.48 Mbp_chr5_57482601_F TCGTCGGCAGCGTCATCTCCCACGTAGCAGTCCT 60.0 0.27
mm4__chr5 57.48 Mbp_chr5_57482601_R GTCTCGTGGGCTCGGATGGGTCAGGGATGTGCAAG 60.0 0.27
mm4__chr9 2.02 Mbp_chr9_2016823_F TCGTCGGCAGCGTCCCCAACCTGGTTTACCCCTT 59.5 0.26
mm4__chr9 2.02 Mbp_chr9_2016823_R GTCTCGTGGGCTCGGTTTAGGGAAGTGCATCGCGA 59.7 0.26
mm3__chr15 43.99 Mbp_chr15_43990079_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 0.26
mm3__chr15 43.99 Mbp_chr15_43990079_R GTCTCGTGGGCTCGGTGTCGTCGACAATGGCTCTG 60.3 0.26
mm0__chr11 1.80 Mbp_chr11_1803992_F TCGTCGGCAGCGTCCGCCACCCACATAGAAGTCC 60.4 0.25
mm0__chr11 1.80 Mbp_chr11_1803992_R GTCTCGTGGGCTCGGAACACCTCTGGCATGTGCAA 60.4 0.25
mm4__chr17 35.73 Mbp_chr17_35731954_F TCGTCGGCAGCGTCGAGGAGGGGAAGCGGTCA 60.6 0.25
mm4__chr17 35.73 Mbp_chr17_35731954_R GTCTCGTGGGCTCGGCCCCTCCTAGTCCCGGAC 60.1 0.25
mm4__chr19 17.21 Mbp_chr19_17205904_F TCGTCGGCAGCGTCCTTGTGCGGGACCAGGAG 60.0 0.25
mm4__chr19 17.21 Mbp_chr19_17205904_R GTCTCGTGGGCTCGGCGAAGTGGCCAGGCTCAA 59.9 0.25
mm1__chr5 77.78 Mbp_chr5_77784966_F TCGTCGGCAGCGTCCTCGTCATTGACAACGGCTC 59.2 0.25
mm1__chr5 77.78 Mbp_chr5_77784966_R GTCTCGTGGGCTCGGAATCCTTCTGACCCATGCCC 59.7 0.25
mm3__chr1 13.90 Mbp_chr1_13904163_F TCGTCGGCAGCGTCTGAAAGGCTGGGTAGAGGGA 59.8 0.24
mm3__chr1 13.90 Mbp_chr1_13904163_R GTCTCGTGGGCTCGGCTGTGCTGAGGAAGGTGCAT 60.3 0.24
mm4__chr6 157.56 Mbp_chr6_157563163_F TCGTCGGCAGCGTCTTGCTCCCACTACAAGCCTG 59.9 0.23
mm4__chr6 157.56 Mbp_chr6_157563163_R GTCTCGTGGGCTCGGAGGAAGCATTGCTAAGCGGT 60.0 0.23
mm4__chr20 64.20 Mbp_chr20_64202892_F TCGTCGGCAGCGTCGAAAAGAGGCTCGGGAGTCC 60.1 0.23
mm4__chr20 64.20 Mbp_chr20_64202892_R GTCTCGTGGGCTCGGCAGTTCACCTGCAAGCACAC 59.9 0.23
mm4__chr20 49.48 Mbp_chr20_49482194_F TCGTCGGCAGCGTCTCCTCCATCATGTGCAGTCG 59.8 0.23
mm4__chr20 49.48 Mbp_chr20_49482194_R GTCTCGTGGGCTCGGGTGTGCGATGACTACAGCCT 60.1 0.23
mm4__chr10 121.57 Mbp_chr10_121568122_F Primer3: not found at this Tm N.d. 0.22
mm4__chr10 121.57 Mbp_chr10_121568122_R Primer3: not found at this Tm N.d. 0.22
mm4__chr12 48.99 Mbp_chr12_48992370_F Primer3: not found at this Tm N.d. 0.20
mm4__chr12 48.99 Mbp_chr12_48992370_R Primer3: not found at this Tm N.d. 0.20
mm4__chr8 144.48 Mbp_chr8_144475224_F TCGTCGGCAGCGTCAGCATCGGTTGGAGGAAAGG 60.0 0.20
mm4__chr8 144.48 Mbp_chr8_144475224_R GTCTCGTGGGCTCGGTGGCGGTTTCAGATCATCCG 60.4 0.20
mm4__chr8 72.27 Mbp_chr8_72268165_F Primer3: not found at this Tm N.d. 0.19
mm4__chr8 72.27 Mbp_chr8_72268165_R Primer3: not found at this Tm N.d. 0.19
mm4__chrX 46.29 Mbp_chrX_46289026_F Primer3: not found at this Tm N.d. 0.19
mm4__chrX 46.29 Mbp_chrX_46289026_R Primer3: not found at this Tm N.d. 0.19
mm4__chr2 84.59 Mbp_chr2_84590818_F Primer3: not found at this Tm N.d. 0.19
mm4__chr2 84.59 Mbp_chr2_84590818_R Primer3: not found at this Tm N.d. 0.19
mm3__chr11 66.37 Mbp_chr11_66366164_F TCGTCGGCAGCGTCCACCCTGACACTCACTCCAC 59.9 0.18
mm3__chr11 66.37 Mbp_chr11_66366164_R GTCTCGTGGGCTCGGGTGCCTTGTGTTGGTCTTCAC 59.9 0.18
mm4__chr14 105.39 Mbp_chr14_105389144_F TCGTCGGCAGCGTCCACAGTCAGCAGGAGCCTG 60.3 0.18
mm4__chr14 105.39 Mbp_chr14_105389144_R GTCTCGTGGGCTCGGTCCACACGAAGATGCCAAGG 60.3 0.18
mm4__chr1 182.60 Mbp_chr1_182604225_F TCGTCGGCAGCGTCAAGCAGTTGGACAACCTCCC 60.1 0.17
mm4__chr1 182.60 Mbp_chr1_182604225_R GTCTCGTGGGCTCGGGCCACCATCCTGCCTACTAC 59.8 0.17
mm4__chr12 90.07 Mbp_chr12_90070766_F TCGTCGGCAGCGTCTCTTAGCCTCAGGGAAAGCC 59.0 0.17
mm4__chr12 90.07 Mbp_chr12_90070766_R GTCTCGTGGGCTCGGCGGTGTCTTACATCCCAGCA 59.7 0.17
mm4__chr5 98.34 Mbp_chr5_98338822_F TCGTCGGCAGCGTCGCTCCTCATCAACAAGGGCT 60.0 0.17
mm4__chr5 98.34 Mbp_chr5_98338822_R GTCTCGTGGGCTCGGTTTTGACCCATGCCCACCAT 60.1 0.17
mm3__chr1 115.69 Mbp_chr1_115685394_F Primer3: not found at this Tm N.d. 0.16
mm3__chr1 115.69 Mbp_chr1_115685394_R Primer3: not found at this Tm N.d. 0.16
mm4__chr22 45.67 Mbp_chr22_45674067_F Primer3: not found at this Tm N.d. 0.15
mm4__chr22 45.67 Mbp_chr22_45674067_R Primer3: not found at this Tm N.d. 0.15
mm4__chr6 109.44 Mbp_chr6_109441904_F TCGTCGGCAGCGTCGGCTAGTCCCAGCAGTAGAA 58.5 0.15
mm4__chr6 109.44 Mbp_chr6_109441904_R GTCTCGTGGGCTCGGACTGCCAATGATTCCGCTGA 60.0 0.15
mm4__chr1 181.02 Mbp_chr1_181022684_F TCGTCGGCAGCGTCAAGAAGGGGTCCTCCATGGA 59.8 0.15
mm4__chr1 181.02 Mbp_chr1_181022684_R GTCTCGTGGGCTCGGCTTCACCCAGACTAAGGCGC 60.7 0.15
mm4__chr11 62.77 Mbp_chr11_62766151_F TCGTCGGCAGCGTCTTCTGTGCTCTTCTGCCAGG 59.9 0.15
mm4__chr11 62.77 Mbp_chr11_62766151_R GTCTCGTGGGCTCGGGAGCACAGAACCAACCTCCA 59.8 0.15
mm4__chrX 47.32 Mbp_chrX_47316241_F TCGTCGGCAGCGTCTCTGATAAAAGCTTCAGTGAAAGGT 58.7 0.14
mm4__chrX 47.32 Mbp_chrX_47316241_R GTCTCGTGGGCTCGGAGGAAACAAGAGCGTCTGGG 59.9 0.14
mm4__chr18 46.12 Mbp_chr18_46122330_F Primer3: not found at this Tm N.d. 0.14
mm4__chr18 46.12 Mbp_chr18_46122330_R Primer3: not found at this Tm N.d. 0.14
mm4__chr1 166.94 Mbp_chr1_166936689_F Primer3: not found at this Tm N.d. 0.14
mm4__chr1 166.94 Mbp_chr1_166936689_R Primer3: not found at this Tm N.d. 0.14
mm4__chr17 56.51 Mbp_chr17_56511279_F TCGTCGGCAGCGTCCGCCTCTCCCATGTCAGAAA 59.7 0.14
mm4__chr17 56.51 Mbp_chr17_56511279_R GTCTCGTGGGCTCGGGTACACAGTGGGGCAGGATG 60.3 0.14
mm4__chrX 9.84 Mbp_chrX_9842801_F Primer3: not found at this Tm N.d. 0.14
mm4__chrX 9.84 Mbp_chrX_9842801_R Primer3: not found at this Tm N.d. 0.14
mm4__chr11 61.72 Mbp_chr11_61715285_F TCGTCGGCAGCGTCACTGCTGTGAGGACTGAAGC 59.9 0.13
mm4__chr11 61.72 Mbp_chr11_61715285_R GTCTCGTGGGCTCGGCCTGGGGATGGGATAGAGGT 59.8 0.13
mm4__chr2 239.87 Mbp_chr2_239865521_F TCGTCGGCAGCGTCGCCCCTCACCACGGATCC 62.1 0.13
mm4__chr2 239.87 Mbp_chr2_239865521_R GTCTCGTGGGCTCGGCCCGAGTCCAGCTGAAGC 60.1 0.13
mm4__chr16 5.95 Mbp_chr16_5948337_F TCGTCGGCAGCGTCGGCTTTGCTGCTGCTGATTT 60.0 0.12
mm4__chr16 5.95 Mbp_chr16_5948337_R GTCTCGTGGGCTCGGATCAATGTCCCCACTGCTGG 60.0 0.12
mm4__chr17 81.51 Mbp_chr17_81512251_F TCGTCGGCAGCGTCCCATCCACTCACCTGGTGTC 60.0 0.12
mm4__chr17 81.51 Mbp_chr17_81512251_R GTCTCGTGGGCTCGGATGGAAGAAGAGATCGCCGC 60.2 0.12
mm4__chr11 3.67 Mbp_chr11_3666147_F TCGTCGGCAGCGTCGCAGCAGATCCCTGTGACTT 60.0 0.12
mm4__chr11 3.67 Mbp_chr11_3666147_R GTCTCGTGGGCTCGGCGTGTGATGGACCGCTTCTT 60.6 0.12
mm4__chr12 106.08 Mbp_chr12_106084048_F TCGTCGGCAGCGTCGGAGGGTGAGCAAAGGGAAA 59.8 0.12
mm4__chr12 106.08 Mbp_chr12_106084048_R GTCTCGTGGGCTCGGGCGTCAAGACAGTGAGAGCA 60.3 0.12
mm4__chr5 60.21 Mbp_chr5_60210810_F TCGTCGGCAGCGTCCCAGGTAGTTCTGGTAGGCT 58.1 0.12
mm4__chr5 60.21 Mbp_chr5_60210810_R GTCTCGTGGGCTCGGTGGCCTGCCATAAAAGAGCA 59.9 0.12
mm4__chr9 34.38 Mbp_chr9_34381016_F TCGTCGGCAGCGTCCCCGAGCAAGCACTTTCGTA 60.6 0.11
mm4__chr9 34.38 Mbp_chr9_34381016_R GTCTCGTGGGCTCGGCATGCTGAACTCACTGTCGC 59.5 0.11
mm4__chr7 143.14 Mbp_chr7_143140943_F Primer3: not found at this Tm N.d. 0.11
mm4__chr7 143.14 Mbp_chr7_143140943_R Primer3: not found at this Tm N.d. 0.11
mm4__chr16 19.88 Mbp_chr16_19883048_F Primer3: not found at this Tm N.d. 0.11
mm4__chr16 19.88 Mbp_chr16_19883048_R Primer3: not found at this Tm N.d. 0.11
mm3__chr10 3.54 Mbp_chr10_3536132_F TCGTCGGCAGCGTCGCTGTCCTAGAAGCCTCACC 59.8 0.11
mm3__chr10 3.54 Mbp_chr10_3536132_R GTCTCGTGGGCTCGGTGGGTTTCTGCAGCAGGAAT 59.8 0.11
mm4__chr2 10.18 Mbp_chr2_10183466_F Primer3: not found at this Tm N.d. 0.11
mm4__chr2 10.18 Mbp_chr2_10183466_R Primer3: not found at this Tm N.d. 0.11
mm3__chr22 49.86 Mbp_chr22_49856680_F Primer3: not found at this Tm N.d. 0.10
mm3__chr22 49.86 Mbp_chr22_49856680_R Primer3: not found at this Tm N.d. 0.10
mm3__chr22 49.86 Mbp_chr22_49856632_F TCGTCGGCAGCGTCCAGGGGACATGCAGCCTAAA 60.0 0.10
mm3__chr22 49.86 Mbp_chr22_49856632_R GTCTCGTGGGCTCGGGGCCTTCACCTAGAACGAGG 59.8 0.10
mm4__chr22 37.31 Mbp_chr22_37309944_F TCGTCGGCAGCGTCGTAGAGTCCAGCAGGGCAAC 60.3 0.10
mm4__chr22 37.31 Mbp_chr22_37309944_R GTCTCGTGGGCTCGGTGAGGCATCTGTCCTCACCT 60.2 0.10
mm4__chr19 23.28 Mbp_chr19_23283033_F TCGTCGGCAGCGTCTTCAGGTACTTAGGAGGCCG 58.5 0.10
mm4__chr19 23.28 Mbp_chr19_23283033_R GTCTCGTGGGCTCGGTGTGGCATTGATCTCCCTGG 59.7 0.10
mm3__chr16 8.32 Mbp_chr16_8318066_F TCGTCGGCAGCGTCGCCCCAAAGCATGAATGTGG 60.1 0.10
mm3__chr16 8.32 Mbp_chr16_8318066_R GTCTCGTGGGCTCGGTGTTCAATGTGGCTGGCAAA 58.8 0.10
mm4__chr1 223.75 Mbp_chr1_223748066_F TCGTCGGCAGCGTCCCTCTGACCACAGGTTCCCT 60.8 0.10
mm4__chr1 223.75 Mbp_chr1_223748066_R GTCTCGTGGGCTCGGCCACGGGCCTATTAGCTCTG 59.9 0.10
mm4__chr3 184.77 Mbp_chr3_184773902_F TCGTCGGCAGCGTCCCCTTTGGCGAGTCACTGTC 60.9 0.09
mm4__chr3 184.77 Mbp_chr3_184773902_R GTCTCGTGGGCTCGGCTCTCCCTTCAAGGCCTGTG 60.0 0.09
mm4__chr15 72.36 Mbp_chr15_72361900_F Primer3: not found at this Tm N.d. 0.09
mm4__chr15 72.36 Mbp_chr15_72361900_R Primer3: not found at this Tm N.d. 0.09
mm4__chr12 48.78 Mbp_chr12_48776613_F TCGTCGGCAGCGTCCACCTGTCACCTCACGTACC 60.0 0.09
mm4__chr12 48.78 Mbp_chr12_48776613_R GTCTCGTGGGCTCGGTGCATCTGGGTCTGTCCTTG 59.6 0.09
mm4__chr20 420.58 Kbp_chr20_420581_F Primer3: not found at this Tm N.d. 0.09
mm4__chr20 420.58 Kbp_chr20_420581_R Primer3: not found at this Tm N.d. 0.09
mm4__chr4 16.56 Mbp_chr4_16564033_F TCGTCGGCAGCGTCCTTGCAGGGTTACAGCTGGG 60.9 0.09
mm4__chr4 16.56 Mbp_chr4_16564033_R GTCTCGTGGGCTCGGGCTGTGAGTCTTTTGAGTATCTGG 59.3 0.09
mm4__chr20 13.12 Mbp_chr20_13115940_F Primer3: not found at this Tm N.d. 0.08
mm4__chr20 13.12 Mbp_chr20_13115940_R Primer3: not found at this Tm N.d. 0.08
mm4__chr10 129.18 Mbp_chr10_129179423_F TCGTCGGCAGCGTCATCCCTTCTGAGCACAGTGC 60.0 0.08
mm4__chr10 129.18 Mbp_chr10_129179423_R GTCTCGTGGGCTCGGTGTTTCAAACACGTCCCTGC 59.2 0.08
mm4__chr7 21.94 Mbp_chr7_21940620_F Primer3: not found at this Tm N.d. 0.08
mm4__chr7 21.94 Mbp_chr7_21940620_R Primer3: not found at this Tm N.d. 0.08
mm4__chr16 5.31 Mbp_chr16_5307364_F Primer3: not found at this Tm N.d. 0.08
mm4__chr16 5.31 Mbp_chr16_5307364_R Primer3: not found at this Tm N.d. 0.08
mm4__chr10 125.02 Mbp_chr10_125015472_F TCGTCGGCAGCGTCGCAGGAAACGTGACGTCTGT 60.8 0.08
mm4__chr10 125.02 Mbp_chr10_125015472_R GTCTCGTGGGCTCGGAACTGCAAGACCGGGAGTG 59.9 0.08
mm4__chr10 16.73 Mbp_chr10_16730109_F Primer3: not found at this Tm N.d. 0.08
mm4__chr10 16.73 Mbp_chr10_16730109_R Primer3: not found at this Tm N.d. 0.08
mm4__chr1 74.86 Mbp_chr1_74855867_F TCGTCGGCAGCGTCCCATATCCATGGCCGCTGG 60.6 0.08
mm4__chr1 74.86 Mbp_chr1_74855867_R GTCTCGTGGGCTCGGACTGGTAGGATTCTGAACCAAGT 59.0 0.08
mm4__chr2 239.03 Mbp_chr2_239026894_F Primer3: not found at this Tm N.d. 0.08
mm4__chr2 239.03 Mbp_chr2_239026894_R Primer3: not found at this Tm N.d. 0.08
mm2__chr22 41.08 Mbp_chr22_41075136_F TCGTCGGCAGCGTCGGAGTCCTTCTGACCCATGC 60.1 0.08
mm2__chr22 41.08 Mbp_chr22_41075136_R GTCTCGTGGGCTCGGTGTTGACAACAGCTCTGGCA 60.1 0.08
mm4__chr22 20.27 Mbp_chr22_20268453_F TCGTCGGCAGCGTCCATTCCTGAAGAGGCGGCG 61.1 0.07
mm4__chr22 20.27 Mbp_chr22_20268453_R GTCTCGTGGGCTCGGCCTCGGGACAAAGCCCTTG 60.6 0.07
mm4__chr22 49.86 Mbp_chr22_49856824_F TCGTCGGCAGCGTCTTCCCACCCCTCGTTCTAGG 60.6 0.07
mm4__chr22 49.86 Mbp_chr22_49856824_R GTCTCGTGGGCTCGGGCTGCTGAAAAGCCTTCACC 60.0 0.07
mm4__chr22 49.86 Mbp_chr22_49856776_F Primer3: not found at this Tm N.d. 0.07
mm4__chr22 49.86 Mbp_chr22_49856776_R Primer3: not found at this Tm N.d. 0.07
mm4__chr22 39.35 Mbp_chr22_39350559_F TCGTCGGCAGCGTCCCAGGGTTTAAGGTGGCCTT 59.8 0.07
mm4__chr22 39.35 Mbp_chr22_39350559_R GTCTCGTGGGCTCGGCTAGGCAATACGAACCCGGT 59.5 0.07
mm4__chr10 126.16 Mbp_chr10_126156740_F TCGTCGGCAGCGTCTTGCGTAGGGATCCGACAAC 60.1 0.07
mm4__chr10 126.16 Mbp_chr10_126156740_R GTCTCGTGGGCTCGGCAGACTCCAGGCCTTTCAGG 60.0 0.07
mm4__chr16 2.08 Mbp_chr16_2084849_F Primer3: not found at this Tm N.d. 0.07
mm4__chr16 2.08 Mbp_chr16_2084849_R Primer3: not found at this Tm N.d. 0.07
mm4__chr2 5.24 Mbp_chr2_5236307_F Primer3: not found at this Tm N.d. 0.07
mm4__chr2 5.24 Mbp_chr2_5236307_R Primer3: not found at this Tm N.d. 0.07
mm4__chr10 4.10 Mbp_chr10_4096638_F Primer3: not found at this Tm N.d. 0.06
mm4__chr10 4.10 Mbp_chr10_4096638_R Primer3: not found at this Tm N.d. 0.06
mm4__chr15 69.39 Mbp_chr15_69393670_F TCGTCGGCAGCGTCGGAGAGAACCACCTTCCCCT 60.5 0.06
mm4__chr15 69.39 Mbp_chr15_69393670_R GTCTCGTGGGCTCGGTCAACCCTGATTTTCGGGCA 59.8 0.06
mm4__chr5 134.19 Mbp_chr5_134189835_F TCGTCGGCAGCGTCCTTGGCTTCTGGCTCCTCTC 60.1 0.06
mm4__chr5 134.19 Mbp_chr5_134189835_R GTCTCGTGGGCTCGGTCAGCTCTTTAGGCTGACAGT 58.7 0.06
mm4__chr12 52.00 Mbp_chr12_52003256_F TCGTCGGCAGCGTCGCCCCTCATTCCTGACAGAC 60.1 0.06
mm4__chr12 52.00 Mbp_chr12_52003256_R GTCTCGTGGGCTCGGTGGGTACTGTCACTGCTCCT 60.1 0.06
mm3__chr9 135.08 Mbp_chr9_135080248_F TCGTCGGCAGCGTCGGATGAGCTGTCTTGTCCCC 60.1 0.06
mm3__chr9 135.08 Mbp_chr9_135080248_R GTCTCGTGGGCTCGGTCCTCCCAGCAAGTCCAAAA 59.1 0.06
mm3__chr3 38.35 Mbp_chr3_38349128_F Primer3: not found at this Tm N.d. 0.06
mm3__chr3 38.35 Mbp_chr3_38349128_R Primer3: not found at this Tm N.d. 0.06
mm4__chr11 58.23 Mbp_chr11_58228391_F TCGTCGGCAGCGTCAGGGTGAAGATTAAGGCGGTG 60.0 0.06
mm4__chr11 58.23 Mbp_chr11_58228391_R GTCTCGTGGGCTCGGACCGCTATGTGGCTATCTGC 59.9 0.06
mm4__chrX 52.38 Mbp_chrX_52380878_F TCGTCGGCAGCGTCGGTGCCGGATAAGGGAAGAA 59.4 0.06
mm4__chrX 52.38 Mbp_chrX_52380878_R GTCTCGTGGGCTCGGTGGGCAGTTAGTTCCATATGCT 59.4 0.06
mm4__chr22 38.74 Mbp_chr22_38739784_F TCGTCGGCAGCGTCACCTTCTTTCTGCAGCGTCA 59.8 0.05
mm4__chr22 38.74 Mbp_chr22_38739784_R GTCTCGTGGGCTCGGGAGGAGATGCAAGCTCAGCT 59.8 0.05
mm3__chr1 33.99 Mbp_chr1_33985146_F TCGTCGGCAGCGTCACAGCTTCAAGGCTTCACCA 59.8 0.05
mm3__chr1 33.99 Mbp_chr1_33985146_R GTCTCGTGGGCTCGGTGGATGGGGAAGGAAGGCTA 59.9 0.05
mm4__chr2 60.06 Mbp_chr2_60064517_F Primer3: not found at this Tm N.d. 0.05
mm4__chr2 60.06 Mbp_chr2_60064517_R Primer3: not found at this Tm N.d. 0.05
mm4__chr6 83.51 Mbp_chr6_83511518_F Primer3: not found at this Tm N.d. 0.05
mm4__chr6 83.51 Mbp_chr6_83511518_R Primer3: not found at this Tm N.d. 0.05
mm4__chr11 12.51 Mbp_chr11_12513539_F TCGTCGGCAGCGTCGGCATCACCTAACCTCTGCA 59.7 0.05
mm4__chr11 12.51 Mbp_chr11_12513539_R GTCTCGTGGGCTCGGGTTAGCCTGGGTGGGAAGAC 60.0 0.05
mm2__chr5 54.95 Mbp_chr5_54950598_F Primer3: not found at this Tm N.d. 0.05
mm2__chr5 54.95 Mbp_chr5_54950598_R Primer3: not found at this Tm N.d. 0.05
mm4__chr4 27.59 Mbp_chr4_27586206_F Primer3: not found at this Tm N.d. 0.05
mm4__chr4 27.59 Mbp_chr4_27586206_R Primer3: not found at this Tm N.d. 0.05
mm4__chr19 18.96 Mbp_chr19_18955057_F Primer3: not found at this Tm N.d. 0.05
mm4__chr19 18.96 Mbp_chr19_18955057_R Primer3: not found at this Tm N.d. 0.05
mm4__chr12 31.12 Mbp_chr12_31117562_F Primer3: not found at this Tm N.d. 0.05
mm4__chr12 31.12 Mbp_chr12_31117562_R Primer3: not found at this Tm N.d. 0.05
mm3__chr16 17.65 Mbp_chr16_17652336_F TCGTCGGCAGCGTCGGCAAGCTATCCCCACCAAT 60.1 0.05
mm3__chr16 17.65 Mbp_chr16_17652336_R GTCTCGTGGGCTCGGTCTGCTTCAAAGACTGCCGA 59.6 0.05
mm4__chr16 57.12 Mbp_chr16_57116065_F TCGTCGGCAGCGTCTTGTCTGGCTGGCTTCTGAG 59.9 0.05
mm4__chr16 57.12 Mbp_chr16_57116065_R GTCTCGTGGGCTCGGAAAGGCATGGCTTCTCCCTC 60.0 0.05
mm4__chrX 48.87 Mbp_chrX_48868046_F Primer3: not found at this Tm N.d. 0.04
mm4__chrX 48.87 Mbp_chrX_48868046_R Primer3: not found at this Tm N.d. 0.04
mm4__chr1 44.13 Mbp_chr1_44129873_F TCGTCGGCAGCGTCGAAACATTGTTGGGCCCGAC 60.0 0.04
mm4__chr1 44.13 Mbp_chr1_44129873_R GTCTCGTGGGCTCGGCCAGCAATGACTGGGACTCA 59.6 0.04
mm4__chr2 26.51 Mbp_chr2_26513729_F TCGTCGGCAGCGTCCCTTCCCAGGAGAGGATGGA 60.0 0.04
mm4__chr2 26.51 Mbp_chr2_26513729_R GTCTCGTGGGCTCGGGTCAGTGCCTCACCTCTGAC 60.0 0.04
mm4__chrX 72.02 Mbp_chrX_72017134_F TCGTCGGCAGCGTCAAACAACCCACCCCAGAAGG 60.1 0.04
mm4__chrX 72.02 Mbp_chrX_72017134_R GTCTCGTGGGCTCGGTCCAGAACATTCAGGCCCAA 59.2 0.04
mm4__chr19 16.02 Mbp_chr19_16019304_F TCGTCGGCAGCGTCCAGCTGAGCCACCCTACAG 59.7 0.04
mm4__chr19 16.02 Mbp_chr19_16019304_R GTCTCGTGGGCTCGGCAAGTTCTGCTGCATCCCTG 59.1 0.04
mm4__chr16 88.63 Mbp_chr16_88632241_F Primer3: not found at this Tm N.d. 0.04
mm4__chr16 88.63 Mbp_chr16_88632241_R Primer3: not found at this Tm N.d. 0.04
mm4__chr14 104.68 Mbp_chr14_104681988_F TCGTCGGCAGCGTCGAGCTGTCACTCTCCCAAGG 59.7 0.04
mm4__chr14 104.68 Mbp_chr14_104681988_R GTCTCGTGGGCTCGGCTGCTGGCTCTTCTGGGAC 60.0 0.04
mm4__chr18 51.50 Mbp_chr18_51497520_F Primer3: not found at this Tm N.d. 0.04
mm4__chr18 51.50 Mbp_chr18_51497520_R Primer3: not found at this Tm N.d. 0.04
mm4__chrX 44.77 Mbp_chrX_44765433_F TCGTCGGCAGCGTCACCTCTTCTGACCCTGGTGT 60.1 0.04
mm4__chrX 44.77 Mbp_chrX_44765433_R GTCTCGTGGGCTCGGTCTGGGGAGAGGAAAGCCAA 60.4 0.04
mm3__chr10 129.19 Mbp_chr10_129192602_F TCGTCGGCAGCGTCTGGTGACAGCACAGCAGTAG 59.9 0.04
mm3__chr10 129.19 Mbp_chr10_129192602_R GTCTCGTGGGCTCGGTGGCAAGATCCAATCTGCCC 60.3 0.04
mm4__chr16 50.63 Mbp_chr16_50633528_F TCGTCGGCAGCGTCAGAGGGGCAAGAAACAAGCC 60.5 0.04
mm4__chr16 50.63 Mbp_chr16_50633528_R GTCTCGTGGGCTCGGCGGTGCTTGTGCTTCTTGTG 60.3 0.04
mm4__chr1 202.87 Mbp_chr1_202873371_F TCGTCGGCAGCGTCTGACAATGGCTCCAGCACAT 59.9 0.04
mm4__chr1 202.87 Mbp_chr1_202873371_R GTCTCGTGGGCTCGGGGAGTCCTTCTGGCCTATGC 59.8 0.04
mm3__chr5 135.48 Mbp_chr5_135482444_F TCGTCGGCAGCGTCTGAGATCTTCAGAGGCAAGGC 59.7 0.03
mm3__chr5 135.48 Mbp_chr5_135482444_R GTCTCGTGGGCTCGGGCTGAGCAGAGATGATGACAGT 60.1 0.03
mm3__chr1 239.84 Mbp_chr1_239844891_F TCGTCGGCAGCGTCTGATAACTGTGGACAGAAGAAGT 57.1 0.03
mm3__chr1 239.84 Mbp_chr1_239844891_R GTCTCGTGGGCTCGGGCTTCCATCTGGCAGTTGGA 60.3 0.03
mm4__chr1 1.20 Mbp_chr1_1203410_F TCGTCGGCAGCGTCGCCAGGAGTTCCCAGAATGG 60.3 0.03
mm4__chr1 1.20 Mbp_chr1_1203410_R GTCTCGTGGGCTCGGGAGTGGATGCATGCTGGGT 60.0 0.03
mm4__chr4 6.06 Mbp_chr4_6062264_F Primer3: not found at this Tm N.d. 0.03
mm4__chr4 6.06 Mbp_chr4_6062264_R Primer3: not found at this Tm N.d. 0.03
mm4__chr20 12.42 Mbp_chr20_12415467_F TCGTCGGCAGCGTCAGGTGAGCTGCTAGGTAAAACC 60.0 0.03
mm4__chr20 12.42 Mbp_chr20_12415467_R GTCTCGTGGGCTCGGAGGGCTTGGTCTGTGATTGG 59.9 0.03
mm3__chr15 34.79 Mbp_chr15_34794699_F TCGTCGGCAGCGTCGGGAAGTTTACCTGGTGCCG 60.9 0.03
mm3__chr15 34.79 Mbp_chr15_34794699_R GTCTCGTGGGCTCGGATGTGTGACGACGAGGAGAC 59.4 0.03
mm4__chr2 191.01 Mbp_chr2_191006287_F Primer3: not found at this Tm N.d. 0.03
mm4__chr2 191.01 Mbp_chr2_191006287_R Primer3: not found at this Tm N.d. 0.03
mm4__chr8 37.97 Mbp_chr8_37965319_F Primer3: not found at this Tm N.d. 0.03
mm4__chr8 37.97 Mbp_chr8_37965319_R Primer3: not found at this Tm N.d. 0.03
mm4__chr6 101.43 Mbp_chr6_101431434_F TCGTCGGCAGCGTCTGATGCTGTCCTGTGTGCTT 59.8 0.03
mm4__chr6 101.43 Mbp_chr6_101431434_R GTCTCGTGGGCTCGGCGGCTTTGCTGGGGATGA 60.0 0.03
mm4__chr17 74.75 Mbp_chr17_74748762_F TCGTCGGCAGCGTCGTTCCTGGGACACCTGCTTG 60.8 0.03
mm4__chr17 74.75 Mbp_chr17_74748762_R GTCTCGTGGGCTCGGGACTTGGGGTTCCGATGGG 60.0 0.03
mm4__chr12 125.70 Mbp_chr12_125698369_F TCGTCGGCAGCGTCTCCCTGTGACTGGGTCTCTT 59.8 0.03
mm4__chr12 125.70 Mbp_chr12_125698369_R GTCTCGTGGGCTCGGCACAACCCTCCGTCCCAATT 60.2 0.03
mm4__chr16 54.60 Mbp_chr16_54595176_F Primer3: not found at this Tm N.d. 0.03
mm4__chr16 54.60 Mbp_chr16_54595176_R Primer3: not found at this Tm N.d. 0.03
mm4__chr15_KI270727v1_random 283.40 Kbp_chr15_KI270727v1_random_283402_F Primer3: not found at this Tm N.d. 0.02
mm4__chr15_KI270727v1_random 283.40 Kbp_chr15_KI270727v1_random_283402_R Primer3: not found at this Tm N.d. 0.02
mm4__chr15 21.99 Mbp_chr15_21993600_F Primer3: not found at this Tm N.d. 0.02
mm4__chr15 21.99 Mbp_chr15_21993600_R Primer3: not found at this Tm N.d. 0.02
mm4__chr15 21.56 Mbp_chr15_21556307_F Primer3: not found at this Tm N.d. 0.02
mm4__chr15 21.56 Mbp_chr15_21556307_R Primer3: not found at this Tm N.d. 0.02
mm4__chr15 20.98 Mbp_chr15_20982568_F Primer3: not found at this Tm N.d. 0.02
mm4__chr15 20.98 Mbp_chr15_20982568_R Primer3: not found at this Tm N.d. 0.02
mm4__chr4 169.38 Mbp_chr4_169383159_F Primer3: not found at this Tm N.d. 0.02
mm4__chr4 169.38 Mbp_chr4_169383159_R Primer3: not found at this Tm N.d. 0.02
mm4__chr19 3.64 Mbp_chr19_3643637_F Primer3: not found at this Tm N.d. 0.02
mm4__chr19 3.64 Mbp_chr19_3643637_R Primer3: not found at this Tm N.d. 0.02
mm4__chr9 129.22 Mbp_chr9_129224886_F TCGTCGGCAGCGTCTGTACCTAGATGGAGGCCCC 60.1 0.02
mm4__chr9 129.22 Mbp_chr9_129224886_R GTCTCGTGGGCTCGGGTCCTGCCTGCCCATGAG 60.1 0.02
mm4__chr6 46.21 Mbp_chr6_46205055_F TCGTCGGCAGCGTCCGGCATGTGAAAAGCAGCTT 60.0 0.02
mm4__chr6 46.21 Mbp_chr6_46205055_R GTCTCGTGGGCTCGGCACGTAGGAGTCCTTCTGGC 59.8 0.02
mm4__chr18 79.77 Mbp_chr18_79771068_F Primer3: not found at this Tm N.d. 0.02
mm4__chr18 79.77 Mbp_chr18_79771068_R Primer3: not found at this Tm N.d. 0.02
mm4__chr10 77.64 Mbp_chr10_77637122_F Primer3: not found at this Tm N.d. 0.02
mm4__chr10 77.64 Mbp_chr10_77637122_R Primer3: not found at this Tm N.d. 0.02
mm4__chr8 139.59 Mbp_chr8_139593369_F Primer3: not found at this Tm N.d. 0.02
mm4__chr8 139.59 Mbp_chr8_139593369_R Primer3: not found at this Tm N.d. 0.02
mm4__chr7 68.00 Mbp_chr7_67998284_F TCGTCGGCAGCGTCTCCAATCCCAACCAAACCGT 59.8 0.02
mm4__chr7 68.00 Mbp_chr7_67998284_R GTCTCGTGGGCTCGGACCTCTCACCTGTAGCCTGA 59.5 0.02
mm4__chr1 10.83 Mbp_chr1_10834499_F TCGTCGGCAGCGTCGCCAGTGAATCCAGGGAGAC 60.1 0.02
mm4__chr1 10.83 Mbp_chr1_10834499_R GTCTCGTGGGCTCGGTATACCATGCAGTGCCACCC 59.8 0.02
mm4__chr1 246.53 Mbp_chr1_246530778_F Primer3: not found at this Tm N.d. 0.02
mm4__chr1 246.53 Mbp_chr1_246530778_R Primer3: not found at this Tm N.d. 0.02
mm4__chr18 23.42 Mbp_chr18_23423372_F Primer3: not found at this Tm N.d. 0.02
mm4__chr18 23.42 Mbp_chr18_23423372_R Primer3: not found at this Tm N.d. 0.02
mm3__chr11 50.00 Mbp_chr11_49996387_F Primer3: not found at this Tm N.d. 0.02
mm3__chr11 50.00 Mbp_chr11_49996387_R Primer3: not found at this Tm N.d. 0.02
mm4__chr9 1.05 Mbp_chr9_1048250_F TCGTCGGCAGCGTCGGAACAGAGGCTTACACCCC 60.0 0.02
mm4__chr9 1.05 Mbp_chr9_1048250_R GTCTCGTGGGCTCGGAGCCCTTCACCAGTTGTTTT 57.8 0.02
mm4__chr16 2.52 Mbp_chr16_2523268_F TCGTCGGCAGCGTCTGAGCCGTCTGTGTTCTAGT 58.3 0.01
mm4__chr16 2.52 Mbp_chr16_2523268_R GTCTCGTGGGCTCGGCACACACCAAGAGAGGGACC 59.9 0.01
mm4__chr4 1.30 Mbp_chr4_1299337_F Primer3: not found at this Tm N.d. 0.01
mm4__chr4 1.30 Mbp_chr4_1299337_R Primer3: not found at this Tm N.d. 0.01
mm4__chr4 7.30 Mbp_chr4_7302476_F Primer3: not found at this Tm N.d. 0.01
mm4__chr4 7.30 Mbp_chr4_7302476_R Primer3: not found at this Tm N.d. 0.01
mm4__chr17 16.42 Mbp_chr17_16417144_F TCGTCGGCAGCGTCCAACATCAAAAGGGTGGCCC 59.6 0.01
mm4__chr17 16.42 Mbp_chr17_16417144_R GTCTCGTGGGCTCGGGTAGGTCCACCCACTGTGTG 59.9 0.01
mm3__chr3 195.76 Mbp_chr3_195763324_F TCGTCGGCAGCGTCATTTACTCCGGGACAGCTGC 60.1 0.01
mm3__chr3 195.76 Mbp_chr3_195763324_R GTCTCGTGGGCTCGGACATACTGAGGCCGAGGAGA 59.7 0.01
mm4__chr18 79.77 Mbp_chr18_79771272_F Primer3: not found at this Tm N.d. 0.01
mm4__chr18 79.77 Mbp_chr18_79771272_R Primer3: not found at this Tm N.d. 0.01
mm4__chr10 24.95 Mbp_chr10_24952590_F TCGTCGGCAGCGTCTTTGCATTTACCACGACGCC 59.7 0.01
mm4__chr10 24.95 Mbp_chr10_24952590_R GTCTCGTGGGCTCGGCGCCCTCCCTGCTCCTTC 62.1 0.01
mm4__chr16 19.67 Mbp_chr16_19668372_F TCGTCGGCAGCGTCTCACGGTCAATACCTTGGCA 59.3 0.01
mm4__chr16 19.67 Mbp_chr16_19668372_R GTCTCGTGGGCTCGGTGGAGAGAGAATGTGTGTGCA 59.3 0.01
mm4__chr18 79.77 Mbp_chr18_79771153_F Primer3: not found at this Tm N.d. 0.01
mm4__chr18 79.77 Mbp_chr18_79771153_R Primer3: not found at this Tm N.d. 0.01
mm4__chr19 42.02 Mbp_chr19_42022355_F Primer3: not found at this Tm N.d. 0.01
mm4__chr19 42.02 Mbp_chr19_42022355_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 54.79 Mbp_chr1_54788070_F TCGTCGGCAGCGTCACCTCTCCCTCTTCCATCCC 60.0 0.01
mm4__chr1 54.79 Mbp_chr1_54788070_R GTCTCGTGGGCTCGGCAGTGTGGGCTGTTCTCTGT 59.8 0.01
mm4__chr16 642.28 Kbp_chr16_642276_F TCGTCGGCAGCGTCCCTGTCGAGGTGAGACGC 59.8 0.01
mm4__chr16 642.28 Kbp_chr16_642276_R GTCTCGTGGGCTCGGACATTTCCCCAAGGTCACCC 59.8 0.01
mm4__chr4 37.58 Mbp_chr4_37584789_F TCGTCGGCAGCGTCGTGCAAGGTGTCACTCTGGA 59.8 0.01
mm4__chr4 37.58 Mbp_chr4_37584789_R GTCTCGTGGGCTCGGGGGGAAGGCGAAAGAAAGGA 59.9 0.01
mm4__chr1 88.68 Mbp_chr1_88684930_F TCGTCGGCAGCGTCTCTGCCTACCAGAGGGGAC 59.9 0.01
mm4__chr1 88.68 Mbp_chr1_88684930_R GTCTCGTGGGCTCGGGTTCGCGGGTAGCTCTGAC 60.5 0.01
mm4__chr16 27.82 Mbp_chr16_27823167_F TCGTCGGCAGCGTCTTCAGCAGCATCTGACCCTC 59.7 0.01
mm4__chr16 27.82 Mbp_chr16_27823167_R GTCTCGTGGGCTCGGCCCCACCCCTGAGAAACATC 60.0 0.01
mm4__chr17 47.78 Mbp_chr17_47778530_F Primer3: not found at this Tm N.d. 0.01
mm4__chr17 47.78 Mbp_chr17_47778530_R Primer3: not found at this Tm N.d. 0.01
mm4__chr11 108.59 Mbp_chr11_108593855_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 108.59 Mbp_chr11_108593855_R Primer3: not found at this Tm N.d. 0.00
mm4__chr21 37.30 Mbp_chr21_37298149_F TCGTCGGCAGCGTCCCGTGGTGAACTGGAGACAA 59.8 0.00
mm4__chr21 37.30 Mbp_chr21_37298149_R GTCTCGTGGGCTCGGCCAATGGGCACAGGCTTAATC 59.5 0.00
mm3__chr17 50.15 Mbp_chr17_50152207_F TCGTCGGCAGCGTCTGCATCTCAGGCCTTTCTGC 60.6 0.00
mm3__chr17 50.15 Mbp_chr17_50152207_R GTCTCGTGGGCTCGGTGGAGTGGTAGGAGGGCTC 59.9 0.00
mm4__chr8 137.28 Mbp_chr8_137282603_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 137.28 Mbp_chr8_137282603_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 3.77 Mbp_chr19_3768010_F TCGTCGGCAGCGTCCACTCATGGGCAGGGTAGC 60.1 0.00
mm4__chr19 3.77 Mbp_chr19_3768010_R GTCTCGTGGGCTCGGCAGGGATAGGCCAGTGAGGA 60.3 0.00
mm4__chr2 100.10 Mbp_chr2_100104752_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 100.10 Mbp_chr2_100104752_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 100.84 Mbp_chr2_100835856_F TCGTCGGCAGCGTCGAAGTCACTCAGGGTCACCC 59.6 0.00
mm4__chr2 100.84 Mbp_chr2_100835856_R GTCTCGTGGGCTCGGTTGAGTGCTGAGGTCTGTGC 60.2 0.00
mm4__chr2 61.47 Mbp_chr2_61470321_F TCGTCGGCAGCGTCGAAAGCCTCGTCCTCCGAC 60.1 0.00
mm4__chr2 61.47 Mbp_chr2_61470321_R GTCTCGTGGGCTCGGCTCTGGGCTCTCGGAAAGTT 59.3 0.00
mm4__chr4 186.90 Mbp_chr4_186903195_F TCGTCGGCAGCGTCATTGCCTCTTCTCCTGGGGA 60.2 0.00
mm4__chr4 186.90 Mbp_chr4_186903195_R GTCTCGTGGGCTCGGTGTGGGCAGTGGTATTCTGG 59.6 0.00
mm4__chr7 12.73 Mbp_chr7_12729175_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 12.73 Mbp_chr7_12729175_R Primer3: not found at this Tm N.d. 0.00
mm4__chr17 44.97 Mbp_chr17_44968832_F TCGTCGGCAGCGTCCAGGTCTGCAGGGGACAAAG 60.6 0.00
mm4__chr17 44.97 Mbp_chr17_44968832_R GTCTCGTGGGCTCGGGGTCCCCTCCGTATCCTCTT 60.1 0.00
mm4__chr4 164.20 Mbp_chr4_164197056_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 164.20 Mbp_chr4_164197056_R Primer3: not found at this Tm N.d. 0.00
mm4__chrX 27.70 Mbp_chrX_27704950_F Primer3: not found at this Tm N.d. 0.00
mm4__chrX 27.70 Mbp_chrX_27704950_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 65.92 Mbp_chr11_65916632_F TCGTCGGCAGCGTCTTAGACGTTCCTGGAGGGGT 59.8 0.00
mm4__chr11 65.92 Mbp_chr11_65916632_R GTCTCGTGGGCTCGGAAGTTGTGCCTCACTGCTGA 59.8 0.00
mm4__chr17 9.54 Mbp_chr17_9544017_F TCGTCGGCAGCGTCTGGAACCCACAGGAAAATCAGT 59.8 0.00
mm4__chr17 9.54 Mbp_chr17_9544017_R GTCTCGTGGGCTCGGCAGGTGAACCAGAGTGGGAT 59.0 0.00
mm4__chr15 73.60 Mbp_chr15_73601680_F TCGTCGGCAGCGTCTGCTCTCTAGGTCAAGGGTCA 59.9 0.00
mm4__chr15 73.60 Mbp_chr15_73601680_R GTCTCGTGGGCTCGGAACTCTTGCAGATCTGGGCC 60.0 0.00
mm4__chr11 13.12 Mbp_chr11_13116188_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 13.12 Mbp_chr11_13116188_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529554 CTCCCCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCT
TGCACATGCCGGAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATCCA
mm2__chr22 15.72 Mbp_chr22_15721566 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm2__chr2 131.26 Mbp_chr2_131263635 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGCGCCCCAGGCAGCAGGGCATGATGGGGGGCATGCATCAGAAAGAG
mm2__chr2 130.66 Mbp_chr2_130656829 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCAGCAGGGCATGATGGGGGGCATGCATCAGAAAGAG
mm2__chr2 130.46 Mbp_chr2_130463840 CTCTTTCTGATGCATGCCCCCCATCATGCCCTGCTGCCTGGGGCGCCCCACGATGGAAGGGAAGACAGCCCGGGGGGCAT
CGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm2__chr2 130.08 Mbp_chr2_130075268 CTCTTTCTGATGCATGCCCCCCATCATGCCCTGCTGCCTGGGGCGCCCCACGATGGAAGGGAAGACAGCCCGGGGGGCAT
CGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm2__chr14 19.40 Mbp_chr14_19402805 ATCATGTTCTGGTGCCTGGGGCACCCCACGATGGAAGGGAAGACAGCCCGGGGGGCATCGTCACCTGCAAAGCCGGCCTT
GCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm2__chr14 19.00 Mbp_chr14_18998947 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC
CATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm1__chr6 88.28 Mbp_chr6_88276904 GAGTCCTTCTGACCCATGCCCACCATCACTCCCTGGTGCCTAGGGTGCCCCACAATGGAGGGGAAGACGGCCTGGGGAGC
CTTGCGCATGCTGGAGCAGTTGTCGACGACGACGAGCGCGGTGATAGCATCATCCA
mm3__chr2 131.63 Mbp_chr2_131626218 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCAGGCTGTCTTCCCTTC
TATCGTGGGGCGCCCCAGGCACCAGGGCATGATGGAGGGCATGCATCAGAAGGAGT
mm2__chr2 73.90 Mbp_chr2_73901394 AGGCTTCGCAGGAGATGATGCCCCCCGGGCTGTCTTCCCCTCCATTGTGGGCCGCCCTCGCCACCAGGTGCGTGCTCATC
TGGATA
mm4__chr13 113.93 Mbp_chr13_113934998 GTGAGGACGAGGTATTGGCCCTGTGGGGAGCTGACTGGCTACAGGGAAATATTTTTGAGGAAATCCTGGCCTTCTTCCCT
TCCATTCCCACTGGCTCAAAGGAGCCAAGGCATTCCTCCCTCTTCTGG
mm2__chr18 62.44 Mbp_chr18_62442088 CCTCGTCATCGACAATGGCTCTGGCATGTGCAGGGCTGGCTTCGCAGGCGACAGTGCTCTCCAGGCCGTCTTCCTCTCCA
TCATGGGGCACCCCAGGCACCAGGGTGTGATGGTGGGCATGGGTCAGAAGGACTC
mm2__chr5 131.66 Mbp_chr5_131660013 ATGCCCATCATCACACCCTGGTGCCTCGGGTGCCCCATTATGGAGGGGAAGACGGCCCGGGGGCATCGTTGCCCGTGAAG
CAGACCTTCCACA
mm4__chr5 80.30 Mbp_chr5_80302744 CTCTTGCTCTGGGCCTCATCCACATAGGAGTCCTTCTGACCCATGCCCACCATCATGCCCTGGTGCCATGATGAAGGGGA
AGGCAGCCCGGGGGACACCATCGCTCGCAAAGCTAGCCTTGCATATGCCA
mm4__chr3 23.45 Mbp_chr3_23450065 ATGGCTTTGAGGACCTCAGCAATTTTTTCATTTATTGGTGGCCATTTGTCTGTTATGGGAATTGGCCAGGCCGTCTTCCT
TTTCATCCCTTAAACATTTCAGAAAATGAGATAAAAGCCTGCTTTTATCCCCTTGC
mm4__chr13 113.94 Mbp_chr13_113936389 AGTGAGGATGCGGTATTGGCCCCGTGGGGAGCTGACCGGCTATGGGGAAATATTTTTGAGGAAATCCTGGCCGCCTTCCC
TTCCATTCCCACTGGCTCAAAGGAGCCAAGGCCTTC
mm4__chr12 131.85 Mbp_chr12_131846286 CATCCAGTGTCACCCTCAGCCACATCCCTCCTGCCACCGCCTCTGTCTCCACCCGGCCGTCTCCTCCCCCATCTCCGCAT
CTTCTCTTATAAGGAAGCCGGGCCTTG
mm4__chr8 84.95 Mbp_chr8_84948668 CTCTGGTATGTGCAAGGCCAGAATCCTTGTCAAAGATGCCCCCAGGCCTTCTTCCTTTCCATAGTGGGATGCCCATGGGA
CCAGAGTTTGATGATGGGCATAGGGCAGAAGGACTG
mm4__chr19 49.36 Mbp_chr19_49357523 AAGGAGCTCGTGAACACCACGGACCCTCCCGGGACCCATCCCATCCAGGCCTCCTTCCCCTCCACCATGCTCTCTCTGTG
GCTACTCTGGGTCTAACACAACCACTAGAAAAACTATCCCCAAACAGGCACAGTGAGA
mm4__chr16 88.99 Mbp_chr16_88993395 CCTCACAGCCTCAGCACTTGCTTCCCCCTGCCGGGAGCACCATCCCCAGGGGCCCCAGGACTCCTTCCCCTCCATCCCAA
CACGTGGTTGGGACTAAACAATATGTTTGCAACTTGTTTTGGGGTTCACATGAGGGTTCC
mm4__chr16 89.59 Mbp_chr16_89588865 CCAGGACTATGACAGGTGCGCCCACCACCTTCCCCTCACCCCCTGGTCTCCAGGTCAGCTATGACAGGTGCGCCTGGCCG
TCTTCCCCCTCACCCCCCTGGTCTCCAGGTCAGCTATGACAGGTGCACC
mm4__chr1 214.04 Mbp_chr1_214043658 AGGCTGTATGGTCTGTCAAGCCATTTCTGTCTCCCAGGCCTCCTTCCCCTCCGTCCCCAGGACTGGCTTCCTGCCCATGA
TCATAGAGTCCTTCTACATCTCCCCAAAAATCATTCATAAGAACTCACAGGAGGAGGCAC
mm4__chr2 241.32 Mbp_chr2_241315790 CAACTGCCGTGGGAGGAGCACGGCGCTTGCGCAGAGGCGGACAACGCGGACGACTCTGGGCTTGCGCACTCGGAAGCCGG
CCGCATGGGGGAGGGGAAGAAGGCCAGGTCGCACTGAGCCAGGAAGCAGTT
mm4__chr15 80.60 Mbp_chr15_80596402 AAAGCCGTCATGGGGTCAATAAGAAAATAGGGGTGATGGAGGGGGAGAAGCCCAGGACTGGGAGAATCGCACATGCCCCA
GGGGTTTTCACCAAGGA
mm4__chr1 201.99 Mbp_chr1_201988980 GAAGCCCTTCGTGTACACCCAGGGCCAGGCTGTCCTAAACCGGGCCTTCTTCCCTTGCTTCGACACGCCTGCTGTTAAAT
ACAAGTATTCAGCTCTTATTGAGGTAAGGAGACTAAGGTTAGGTGCACCTGCC
mm1__chr19 9.52 Mbp_chr19_9518229 AGTTAACAATGGCTCCGGCATGTGCAAGGTCAACTTCGTGGGCGACAATGCCCCCCGGGCCATCTTCCCCTCCATCGTGG
GGCATCTGAAGCACCAGGGCGTTATGGTGGGCATCGGTCAGAAGGACTCC
mm4__chr1 215.08 Mbp_chr1_215081839 TCGCCTATTACGACGACAGCAACGTCTGATCTCCTGGCTTTCTTCCCCTCCTTGGTGGGTTCCGTTCCGTCCCCCTGTCG
CTGCGAGTTTGGCTGTCCCTACG
mm4__chr11 75.27 Mbp_chr11_75269534 GTCAGGTTTAGGATGGCCCCAGGGCCCAAGGCTGATTACACTGTGGGGATGCAGGGGAAAGCGGCCGGGAAGACCCAGCC
ACCAGCCAGTGCTTCTGATGGGGCTT
mm4__chr18 48.23 Mbp_chr18_48229011 CAGAGCCTCGAACCATCTGGAAACATAAGCACGGCAGGATTGTGCGAGGCTGGAGGTGAAGACAGCCGGGGTCATGGGAA
GGGAGGCGGAAAAACAAGACAGTGCCAGATCCCAGTC
mm4__chr2 115.16 Mbp_chr2_115162316 GGGCAGAGAGGTAAAGGCTGAAGGTGCCCCGGGGAACCCCGGCGGGCGGCCCACCGAGGGAGGGAGAGGCGGCCGGGACC
AAGGAATGGGGCCTCTTGGTTC
mm4__chr5 57.48 Mbp_chr5_57482601 ATCTCCCACGTAGCAGTCCTTCTGGCCCATGCCTACCATAACGCCCTGGTGTCGAGGACGCCCTATCATGGAGGGGAACA
CAGCCCGGGGGGCATCGTCACCACCAAAGCCTGCCTTGCACATCCCTGACCCAT
mm4__chr9 2.02 Mbp_chr9_2016823 CCCAACCTGGTTTACCCCTTCCTTTGCTCTCCCCCTCCACCCGGCCGTCTTCCCTTCCTCCCGTTTGCGTTGCAAAACAC
GGCCATGCCATCTGCAAAGGTGTCGCGATGCACTTCCCTAAA
mm3__chr15 43.99 Mbp_chr15_43990079 GAGTCCTTCTGACCCATGCCCACCGTCACGCCCTGGTGTCTGGGGCGCCCCACAATGAAGGGGAAGATGGCCCGGGGTGG
GCATCGTCACCTGCAAAGCTGGCCTTGCACATGCCAGAGCCATTGTCGACGACA
mm0__chr11 1.80 Mbp_chr11_1803992 CGCCACCCACATAGAAGTCCTTCTGACCCATGCCCACCGTCATGCCCTGGTGTCTGGGGCACCCCACGATGGAGGGGAAG
ACGGCCCAGGGGGCATCGTCGTCGGTGAAGCTGGCCTTGCACATGCCAGAGGTGTT
mm4__chr17 35.73 Mbp_chr17_35731954 GAGGAGGGGAAGCGGTCAGGGGTGGAGCGGGGCCGGGTCAGGGGCTCGGGGGGTCGGGCCGGGGTCGGGGGAGGAGGAGA
CGGCCTGGGCGAGGGGCGTCCGGGACTAGGAGGGG
mm4__chr19 17.21 Mbp_chr19_17205904 CTTGTGCGGGACCAGGAGGCAGAGGCCCCCAGAGACAGCTGGAGAGGAAGACAGCCAGGCCCAGACCTGACCCCCAACCC
CGGCACTGCAGGGCGAGCCAGGCGTTGAGCCTGGCCACTTCG
mm1__chr5 77.78 Mbp_chr5_77784966 CTCGTCATTGACAACGGCTCCGGCATGTGCAAGGCCAGCTTCACGGGCGACAATGCCGCCCGGGCAGTCTTCCCCTCCAT
CGTTGGGCACCCCAGGCACCAGGGCGTGATGGTGGGCATGGGTCAGAAGGATT
mm3__chr1 13.90 Mbp_chr1_13904163 TGAAAGGCTGGGTAGAGGGATCCAAAGAAGGACTAGGGCAAGGGAGCAGAGGATGGAGGGGCAGAAGGCCGGGCGAGGAG
GTGCCCTGGGATGCTGGAGGCCAGCATGCACCTTCCTCAGCACAG
mm4__chr6 157.56 Mbp_chr6_157563163 TTGCTCCCACTACAAGCCTGGAGGCTGGAGGGGAAGGCAGCCAGGTTGACTCAAGGGCCTGGATGGCAGAAGTACTGGCC
AGGCCTGCCCTCCCAGGAAACACCACGGCCACCGCTTAGCAATGCTTCCT
mm4__chr20 64.20 Mbp_chr20_64202892 GAAAAGAGGCTCGGGAGTCCTGGAGCCCTGGCCGTCTTCCTCTCCCTACAACTCTGGCCCTGAGCACCCAGAGGACATCA
GGAAATAAGAGCTGAGTGTGCTTGCAGGTGAACTG
mm4__chr20 49.48 Mbp_chr20_49482194 TCCTCCATCATGTGCAGTCGCCCAGTGCGGTAGAAGTTGAGGATGGAGGTGAAGGCGCCCGGGTGGCGGTCAAAGAAGTA
CTCGTTGTCGTCGAGGCTGTAGTCATCGCACAC
mm4__chr8 144.48 Mbp_chr8_144475224 AGCATCGGTTGGAGGAAAGGTTGTGGCGAATGGAGTCTTTCCAGCCCTCGTAGTCTTCCCTGAAGAAGGGGAACACGGCC
TGGACCTGACGGATGATCTGAAACCGCCA
mm3__chr11 66.37 Mbp_chr11_66366164 CACCCTGACACTCACTCCACTTCCCAGGACTGGTGGAGCTGGTCACCATGGCTGTGATGGCGGGGAAGACGGACAGGGTG
ACTGTGAAGACCAACACAAGGCAC
mm4__chr14 105.39 Mbp_chr14_105389144 CACAGTCAGCAGGAGCCTGTGCGAGCTGGACCACAGGACAGCCCTGCTTGAGCTGCACACGTCGGAGGGGAAGACAGACG
GGTCCCCTCAGCGGCCCCGCGGCTCCTGTCCTCCACCCTTGGCATCTTCGTGTGGA
mm4__chr1 182.60 Mbp_chr1_182604225 AAGCAGTTGGACAACCTCCCCTTACCTCTCCAGGCAGGTGGTGGGGAAGGCGGCCAGGGTGCGGCACATGGCTGCGGGCG
CAGGGCAGCACGTAGTAGGCAGGATGGTGGC
mm4__chr12 90.07 Mbp_chr12_90070766 TCTTAGCCTCAGGGAAAGCCCTGTATCTACACAATCATATCTCCCTTTCCTAATCAGCATTCCTATGCAGGGGAAGACAG
ACAGGAGGAGGCAAGTAAATCAAAGAATCAAGACTTAGTGCTGGGATGTAAGACACCG
mm4__chr5 98.34 Mbp_chr5_98338822 GCTCCTCATCAACAAGGGCTCTGGCATGTGCAAGGCCGGCTTCACAGGTGACAATGCCCCCGGGCCATCTTCCCCTCTGT
CATGGGTGCCAGGGAGTGATGGTGGGCATGGGTCAAAA
mm4__chr6 109.44 Mbp_chr6_109441904 GGCTAGTCCCAGCAGTAGAAAAAGAAAAAAATAGCTGATCAGAGCTGGAAGACAAGGGAGGGGAAGAAGGCTGGGTGTCT
CTCCCTGTTTTTCTGGTTATTAAGCAGGGCTTGGCTTTCAGCGGAATCATTGGCAGT
mm4__chr1 181.02 Mbp_chr1_181022684 AAGAAGGGGTCCTCCATGGACATGGCGTCCCGGCCCCGGCCGCCTTCACCTCCTCCGCGCACAGGGCGCCCGTGCCTCCC
GGTCTCCCTCCGCCCACCCCGCCTGTTCCCGCAGCTGCCCGCGCCTTAGTCTGGGTGAAG
mm4__chr11 62.77 Mbp_chr11_62766151 TTCTGTGCTCTTCTGCCAGGAAGAAGGGATGGATGGGCAGAAGGCCTGGGGCTGCAGGGTGGAGGTTGGTTCTGTGCTC
mm4__chrX 47.32 Mbp_chrX_47316241 TCTGATAAAAGCTTCAGTGAAAGGTTTTACAAATCAACAGAAAGAATAAACGATGAAGAGGAAAATGGCCTGGGAAAAAA
TGATTTCATACAGATAAGCTTCCCAGACGCTCTTGTTTCCT
mm4__chr17 56.51 Mbp_chr17_56511279 CGCCTCTCCCATGTCAGAAATACTCAGCAGCATGCTTTAGGGAGGCCCATCCCGGCTGTCCACCCCTCCATGGCTGCTAC
CTGCGTTTTACATCACCCTTACCCCCATCCTGCCCCACTGTGTAC
mm4__chr11 61.72 Mbp_chr11_61715285 ACTGCTGTGAGGACTGAAGCCGGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCTATG
GCTGGACCTGGCTGTCATCCCCTCCTTCAGGCACAACCTCTATCCCATCCCCAGG
mm4__chr2 239.87 Mbp_chr2_239865521 GCCCCTCACCACGGATCCCGAGCGCCGGGAAGACGGCCGGGCGCCCAGGAGCGCTCGGGGCCCGCCTGCTGCCCACGCAC
GGTCCAGCACCCGGCTCGGAGGGCACCTCCCGGAGCTTCAGCTGGACTCGGG
mm4__chr16 5.95 Mbp_chr16_5948337 GGCTTTGCTGCTGCTGATTTGTAATTGAGTTCAAGGAGAGGAGAGGACCAAAACATTTGTGGGAGACACATGGCCTTTGG
CTCCTGGCCATCTTCTCCTCCGTGGCTGTGGTCCAGCAGTGGGGACATTGAT
mm4__chr17 81.51 Mbp_chr17_81512251 CCATCCACTCACCTGGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCGTCCCCAGCAAAACCAG
CTTTGCACATGCCGGAGCCATTGTCAATGACCAGCGCGGCGATCTCTTCTTCCAT
mm4__chr11 3.67 Mbp_chr11_3666147 GCAGCAGATCCCTGTGACTTAGTCCCAGCCCTCACAGGGCCTGCACCAGCACCAGGAGGCTCATGACCAGGGCCATGGAG
AAGAAGATGGCCAGGAAGAAGCGGTCCATCACACG
mm4__chr12 106.08 Mbp_chr12_106084048 GGAGGGTGAGCAAAGGGAAATGTCTGACAGATTAAAGAAATGCACCAGCCCGGTGGTCTTCACCTCCATCCTTCAGGCTG
CTCTCACTGTCTTGACGC
mm4__chr5 60.21 Mbp_chr5_60210810 CCAGGTAGTTCTGGTAGGCTACAATGTAGGGGAAGATGACCAGGTGGCCTCAGGGACCAAGAACAGTTTCACTTTGGTGC
TTCTATTTCTAACAGCTGCGTTTCCATCCTAATGGTGGATGCTCTTTTATGGCAGGCCA
mm4__chr9 34.38 Mbp_chr9_34381016 CCCGAGCAAGCACTTTCGTAACCATGGAAGGGCAGGCGGCCGGGCAGCGGGTCCACGCACCCGCATCGGGGCGTGATCCG
CGGCAGCTGCTGGTTCCGCGACAGTGAGTTCAGCATG
mm3__chr10 3.54 Mbp_chr10_3536132 GCTGTCCTAGAAGCCTCACCTGATTGTGAACAAACCCCTGCCAGGCCATGTTCCCCTCCATTGCCAGGAGCCTGTTCATC
TTATTCCTGCTGCAGAAACCCA
mm3__chr22 49.86 Mbp_chr22_49856632 CAGGGGACATGCAGCCTAAATGACCCACACTGGAATCCCGGCCGGCTTCCCATCCCTCGTTCTAGGTGAAGGCCGCGCTG
GAATCCCGGCCGGCTTCCCATCCCTCGTTCTAGGTGAAGGCC
mm4__chr22 37.31 Mbp_chr22_37309944 GTAGAGTCCAGCAGGGCAACCCCAGTGCCCGGCCGTCTGCCCCTGCCTCCCACACACACTTCCGGAAGCCTCTCCAGCAG
GGACAGCCAAGCCGGGGACAGCGCCTCCAGGGCAGGTGAGGACAGATGCCTCA
mm4__chr19 23.28 Mbp_chr19_23283033 TTCAGGTACTTAGGAGGCCGAGATGGATGGGAAGACCACCTGGGCAAGACCAGGGAGATCAATGCCACA
mm3__chr16 8.32 Mbp_chr16_8318066 GCCCCAAAGCATGAATGTGGAAAACCCACCCTGGCCGTCGTCCCCTCCTTCTTTGTCTTCTAGAGTCTCTGTGGCCATTT
GTTTCTTATTTGCCAGCCACATTGAACA
mm4__chr1 223.75 Mbp_chr1_223748066 CCTCTGACCACAGGTTCCCTGGGAGCCCCTGCCACCTGCTGCCCCTGCCACCTTCCCCTCCATCTGCAGTGCTGTGCAGC
CTTCCCCTGCACTCTTGCAGAGCTAATAGGCCCGTGG
mm4__chr3 184.77 Mbp_chr3_184773902 CCCTTTGGCGAGTCACTGTCCCGGGCTCTCTTCCCCTTCATGGCAGCAGCCAGGCCCTCCCTGCACAGGCCTTGAAGGGA
GAG
mm4__chr12 48.78 Mbp_chr12_48776613 CACCTGTCACCTCACGTACCAGCCTGGGAGGATGCAGCCCCAGATCAGCTCCTGGCAGTCTTCCCCTCCCCCAGCCCACA
ACCCAGGCCCTTCACTCCTCTCAGGGCCCAGCGGGCAAGGACAGACCCAGATGCA
mm4__chr4 16.56 Mbp_chr4_16564033 CTTGCAGGGTTACAGCTGGGTCCCAGGCTCTCTTCCCCTCCCTCATTCTCCTTTCTTTTTGAGCTTCCCCCTTCTTAATG
GTCTTCAAGATTTTAATTACCAGATACTCAAAAGACTCACAGC
mm4__chr10 129.18 Mbp_chr10_129179423 ATCCCTTCTGAGCACAGTGCAGGCAAATGCTGGCATGGAAAGGACCCCCAGATGAGCCAGGAAGTCTTCCACTCCATCAT
GAAGAATTGTTCTCTGGAGTGGGGGCCAGCAGGGACGTGTTTGAAACA
mm4__chr10 125.02 Mbp_chr10_125015472 GCAGGAAACGTGACGTCTGTTTTTGAAACAGAGCCTTTGCTGTGCGGTCCCTGGTGTGGCCTGGCCATCCTCCCCTGCCT
CGCTGGGGCCACAGCCGGGCTCCCAGGGCTCCCTCCGTCCACTCCCGGTCTTGCAGTT
mm4__chr1 74.86 Mbp_chr1_74855867 CCATATCCATGGCCGCTGGCCTGTGAAAGACAGAAGTCCTGGTGATGTGGAGGTGAAGACGGCCAAGATAGGTGAACTCA
ATAGTAACCTATTTTAAAACTTGGTTCAGAATCCTACCAGT
mm2__chr22 41.08 Mbp_chr22_41075136 GGAGTCCTTCTGACCCATGCCCACCATCATGACCTGGTGCCTGGTGCACCCCACGATGGAAGGGAAGATGGCCCAGGGGA
CATCGTCACCCGCGAAGCCGGCCTTGCACATGCCAGAGCTGTTGTCAACA
mm4__chr22 20.27 Mbp_chr22_20268453 CATTCCTGAAGAGGCGGCGGCCCCTGCAGGCGGCGGAGGGGATGGCGGCCCGGGCCGGAGCCGGCCGGGGACGCGGGCCC
GAGGGTGTGGCGGCCTAGGCTGGCCCGGTCCCCAAGCGGGCAAGGGCTTTGTCCCGAGG
mm4__chr22 49.86 Mbp_chr22_49856824 TTCCCACCCCTCGTTCTAGGTGAAGGCCGCGCTGGAATCCCGGCCGGCTTCCCACCCCTCGTTCTAGGTGAAGGCCGCGC
TGCAATGCCAGCCAGCTTCCCACCTCATTCTAGGTGAAGGCTTTTCAGCAGC
mm4__chr22 39.35 Mbp_chr22_39350559 CCAGGGTTTAAGGTGGCCTTTTTTGGGGGGTGGATCAGGGCGTGGACCATCTCGGTGGGGTGCATGCCGCGCCTGCGATG
GACGTTGGAGGAGGAGAGGGCCGGGAACCGGGTTCGTATTGCCTAG
mm4__chr10 126.16 Mbp_chr10_126156740 TTGCGTAGGGATCCGACAACTACTGGGCCCTAACGATGGAGCAGGAGTCGGCCAGGCCCAGGACAGGGTGGAAGGAGAGG
GGAAATCCTGGCAGATAATGTCAAGGTCAGGGCCTGAAAGGCCTGGAGTCTG
mm4__chr15 69.39 Mbp_chr15_69393670 GGAGAGAACCACCTTCCCCTCCAGGGAGGGGAAGACCTCCTGGGGGAGACCAGGAACCTCAGTATTTGCTTGACAGTTAT
CTGATGCTTGCCCGAAAATCAGGGTTGA
mm4__chr5 134.19 Mbp_chr5_134189835 CTTGGCTTCTGGCTCCTCTCAGATTAGAAGGTAAGGGTCTTCAGAAGGAGGGGAAGATGGTCAGGTGATGACAGGCTAGC
ATGAGATTTCATTCATTACTACAACTGTCAGCCTAAAGAGCTGA
mm4__chr12 52.00 Mbp_chr12_52003256 GCCCCTCATTCCTGACAGACCAGAGAGCCAGGGCCCCAGGCCCTCTTCCCCCCCACCTCCCCAGCTTAGGAGCAGTGACA
GTACCCA
mm3__chr9 135.08 Mbp_chr9_135080248 GGATGAGCTGTCTTGTCCCCCTGCCAGGCCCTCTTCCCCTCCAGCTTCTCTGCCCCCTCTGTTTTGGACTTGCTGGGAGG
A
mm4__chr11 58.23 Mbp_chr11_58228391 AGGGTGAAGATTAAGGCGGTGAGCTGCAGGGAGGGGAAGAGGGCCAGGCCCAGGGCCCCACCCAGCATCTGCGTGCACAG
CTCGCGGGTCATGATGAGGGTGTAGTGCAGCGGGTGGCAGATAGCCACATAGCGGT
mm4__chrX 52.38 Mbp_chrX_52380878 GGTGCCGGATAAGGGAAGAAGGAGGGCCATAGAGGGGAGGAGGGCCTGGTGAGCATACAGTGAGTTATGAAGGGGGACAC
TATTTGAGGTATTTGAATCCTTAGAGCATATGGAACTAACTGCCCA
mm4__chr22 38.74 Mbp_chr22_38739784 ACCTTCTTTCTGCAGCGTCAGGCTCAGGGAGGCCGCGGCTTCCCTGGCCGACTTGCCCTGCATCTCTGCCACATGGGTGA
GGATCTTGCTCTCCAGCTCTCGCAGCTGAGCTTGCATCTCCTC
mm3__chr1 33.99 Mbp_chr1_33985146 ACAGCTTCAAGGCTTCACCAGCAGGTCGATGGAGGGGAAGCTGTCCTGGAGGGATGGAGCCCTGGGCTGTCTCCCTTTCA
CTATGGAGACTCAAAACTCAGGACTTAGCCTTCCTTCCCCATCCA
mm4__chr11 12.51 Mbp_chr11_12513539 GGCATCACCTAACCTCTGCACACACAGGGCTTTCCCCCTTGCCATCCATGTACCTGTCTGTTCCTCACTGGATGTTGCAC
CTGGGCCTTTTCCCCTCCATCAGCATGAACAGCCTGTCTTCCCACCCAGGCTAAC
mm3__chr16 17.65 Mbp_chr16_17652336 GGCAAGCTATCCCCACCAATAATCATTCACTTTCAGGGTAGCCTCTGAACAGGTATTGACTTTAGAGACTAGCAATTCAT
CCCGGGCCCTCTATCCCTCCATCGAGATTCTTCGGCAGTCTTTGAAGCAGA
mm4__chr16 57.12 Mbp_chr16_57116065 TTGTCTGGCTGGCTTCTGAGCCCACGCGATGCCTGGGAAGCCGGCCAGGCTGGAGGGAGAAGCCATGCCTTT
mm4__chr1 44.13 Mbp_chr1_44129873 GAAACATTGTTGGGCCCGACTGTGCCTTCCACTGAGGCCCAGGCAGTGCTCCCCTCCATGGCTCAGATGTTGCCCCCGCA
AGATGCCCATGACCTTGGGATGCCCCCAGCTGAGTCCCAGTCATTGCTGG
mm4__chr2 26.51 Mbp_chr2_26513729 CCTTCCCAGGAGAGGATGGAGGGCAGGGAGCAGATGCAGGGGAAGCCTGCCTGGGCTCCTAACGGGGTCAGAGGTGAGGC
ACTGAC
mm4__chrX 72.02 Mbp_chrX_72017134 AAACAACCCACCCCAGAAGGGTGCACTCATTCATCATGCCCCTCTATATAGGGGAAGACGGGCTGGGTAGCAGGGCCTAT
TGGGCCTGAATGTTCTGGA
mm4__chr19 16.02 Mbp_chr19_16019304 CAGCTGAGCCACCCTACAGGGAGGGGCGTGGGGGGCAGTGAGGGCCATACCCCTGGGAGGGCGGAGGGGAAGACGGGCAG
GGTGTCAGCCAGGGCATGTCAGGGTGAGGGCAGGGATGCAGCAGAACTTG
mm4__chr14 104.68 Mbp_chr14_104681988 GAGCTGTCACTCTCCCAAGGCGGGAAGTGGGAGGGGCTGGGAGTCTCCAGGGAGGGGAGGAGGGCCTGGAGGAGTTCCCT
GGGGTGGGGGTCCCAGAAGAGCCAGCAG
mm4__chrX 44.77 Mbp_chrX_44765433 ACCTCTTCTGACCCTGGTGTCTCCCAGCTGTGTCCCTGGCTATAGCCCACACTGCACATATTCTAGGCCACCAGGCCCTC
TTCCCCACCATATTAGTCATCAGCTTTCTATTGGCTTTCCTCTCCCCAGA
mm3__chr10 129.19 Mbp_chr10_129192602 TGGTGACAGCACAGCAGTAGCACGGCTGGCCATTTCCTTCTTGTCCATCAGGCCGTCTCCACCTCCATCTGGGGGGCAGA
TTGGATCTTGCCA
mm4__chr16 50.63 Mbp_chr16_50633528 AGAGGGGCAAGAAACAAGCCCCCTCTGGGACCCGCCATCCCTGCGGTGTCCCCCTCCGCCCACCTGGCTGCCAGCCCGGC
CCTCCTCCCCTCCCTAGCCCCCCTCGGGCACAAGAAGCACAAGCACCG
mm4__chr1 202.87 Mbp_chr1_202873371 TGACAATGGCTCCAGCACATGCAAAGCTGGCTTTGCTGGGTATAGTTCCCCGAGCCATGTTCCCCTCCATCACTGGGTAT
CCCTGGCACCAGGGCATGATGGTGGGCATAGGCCAGAAGGACTCC
mm3__chr5 135.48 Mbp_chr5_135482444 TGAGATCTTCAGAGGCAAGGCCGGGTGGAGGGGAAGACTGCCTAGGTCAATGTGGCTGTTACTATCATCACTGTCATCAT
CTCTGCTCAGC
mm3__chr1 239.84 Mbp_chr1_239844891 TGATAACTGTGGACAGAAGAAGTAGAGGGGTGGAGGAGAAGACGGCCAGAACTTTGTACATGGAATGCAGTGTGAACTAA
GGAAAGAGGGCTTATAAAAAGATGTCTTAATTTTTTTTCCAACTGCCAGATGGAAGC
mm4__chr1 1.20 Mbp_chr1_1203410 GCCAGGAGTTCCCAGAATGGGCCCTGGCTGCCCTCCGCTTTGCCCCCTGTGGAGGGGACCACGGCCAGGCATGAGCACGG
TGCCCCAAAGCCCAGCCCCGGGTGCCCCGTCCTCCCCCAGCACCCAGCATGCATCCACTC
mm4__chr20 12.42 Mbp_chr20_12415467 AGGTGAGCTGCTAGGTAAAACCTTGGAGCTCTCCTGGCCAGCCTCCCCTCCCTCGCTCCCACCACCAATCACAGACCAAG
CCCT
mm3__chr15 34.79 Mbp_chr15_34794699 GGGAAGTTTACCTGGTGCCGCGGGCGGCCCACGATGGACGGGAAGACAGCGCGGGGCGCGTCATCGCCCGCAAAGCCGGC
CTTCACCAGCCCAGAGCCGTTGTCGCACACCAGGGCGGTGGTCTCCTCGTCGTCACACAT
mm4__chr6 101.43 Mbp_chr6_101431434 TGATGCTGTCCTGTGTGCTTACTAGAAAGACAAGTCTTTACTCTGACAGAGTTTATATTCTGGTGCTGGGGGCGCCCAAC
AATGGAGGGGAACATGGCTTGGGGGCGTCATCCCCAGCAAAGCCG
mm4__chr17 74.75 Mbp_chr17_74748762 GTTCCTGGGACACCTGCTTGCTTGGCCCGTCCGGCGGCTCAGGGCTTCTCTGCTGCGCTCCCGGTTCGCTGGACGGGAAG
AAGGGCTGGGCCGTCCCGTCCCGTCCCCATCGGAACCCCAAGTC
mm4__chr12 125.70 Mbp_chr12_125698369 TCCCTGTGACTGGGTCTCTTCCCATGCAGCTCTGATACTCTTATAAAAACCATCAATTTCACTCCAACAAGGAAGGAGGG
GAAGAGGCCCTGGAATTGGGACGGAGGGTTGTG
mm4__chr9 129.22 Mbp_chr9_129224886 TGTACCTAGATGGAGGCCCCTCCGCTCCCTGGGCCCAGGGGCGTCCTGTGGTCCAGGCAGGCACCTCCCCTGACCCAGGC
GAACCAGCCCGTCCTCCTCTCCTTCGACTCATGGGCAGGCAGGAC
mm4__chr6 46.21 Mbp_chr6_46205055 CGGCATGTGAAAAGCAGCTTTGCTGGGACAATGCCCTCCGAGCCATATTCCCCTCCATCATCGGGCACCCCCGGCACCAG
GGCGTGATGGTGGGCATGGGCCAGAAGGACTCCTACGTG
mm4__chr7 68.00 Mbp_chr7_67998284 TCCAATCCCAACCAAACCGTAGAAGTGTCCAAAGATGGAGGGGAAGACAGAGAGGAAGTGGGAAATAGCAGATCAGGCTA
CAGGTGAGAGGT
mm4__chr1 10.83 Mbp_chr1_10834499 GCCAGTGAATCCAGGGAGACTGAGGCTAGAGCAGGGAAGGAGGGGAACACGGGCGGGGGGGGGTGGCACTGCATGGTATA
mm4__chr9 1.05 Mbp_chr9_1048250 GGAACAGAGGCTTACACCCCTTTCTCCTCCTGTTTCTCCTCCAGTCCCTCTTCCCCTCAATCTAGTTTCCCAAAGTTAGC
AAACCTACTTTCTTGATTTTGTTTCTTAACATTTAAAACAACTGGTGAAGGGCT
mm4__chr16 2.52 Mbp_chr16_2523268 TGAGCCGTCTGTGTTCTAGTTCTGTCCAGAGCCCCCGCCGTTTTCCCCTCCAGCACAGGATTCATTTCAGGATCATCACT
TGCGTTATATGCCCAAGGGGTCCCTCTCTTGGTGTGTG
mm4__chr17 16.42 Mbp_chr17_16417144 CAACATCAAAAGGGTGGCCCTATTGGTTCCCTGTTCCTCACCCCACAGGCCTCCCTCCCTCTCCTCACCCCTGCTGCTCA
TCCCTCCTGGCCCACTTCCCCTGCATTGGGGCTGGGCTCCCACACAGTGGGTGGACCTAC
mm3__chr3 195.76 Mbp_chr3_195763324 ATTTACTCCGGGACAGCTGCGTGGATGGGCTGTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNT
CTCCTCGGCCTCAGTATGT
mm4__chr10 24.95 Mbp_chr10_24952590 TTTGCATTTACCACGACGCCTCGCCCGTAGTCTGGCGCCTCCTCGCTGCTCCCGGCCATGTTTCTCCCGGGGAACGCGGG
AAGGGAAGACGGCGCGGGAAGGAGCAGGGAGGGCG
mm4__chr16 19.67 Mbp_chr16_19668372 TCACGGTCAATACCTTGGCAGTTTTAGAAAGAACTTTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTGTGCACACACATTCTCTCTCCA
mm4__chr1 54.79 Mbp_chr1_54788070 ACCTCTCCCTCTTCCATCCCGTACCCCCACCACCCTCTTGCCTGACCTGATGTAGAGTGTTGCCATGGTGAAATACCAGC
CCCTTTTCTCCTCCATCGGGATCTAGGGAAACAGAGAACAGCCCACACTG
mm4__chr16 642.28 Kbp_chr16_642276 CCTGTCGAGGTGAGACGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCCCCTCCCCCGCCGGCCGCCCTAGAGCGGAGG
GTGACCTTGGGGAAATGT
mm4__chr4 37.58 Mbp_chr4_37584789 GTGCAAGGTGTCACTCTGGATGTGCCTGCCTGCTTTTCCTCGCCTTCTTGCCCTCCTTCGCCTTTCCTCCCTCTTTTCTT
TCCTCCTTTCTTTCGCCTTCCCC
mm4__chr1 88.68 Mbp_chr1_88684930 TCTGCCTACCAGAGGGGACCCCATCTCTGCAGCCCTGCTGCAGCGGGAGCCTGCTCTCGCCTGGTCCCCGGAGAGGCCGG
CCCGTCGCCCCCTCCCTCGGTGTCAGAGCTACCCGCGAAC
mm4__chr16 27.82 Mbp_chr16_27823167 TTCAGCAGCATCTGACCCTCCCCTTGGGAGGCCCGATTAAGACGAAGGAGGGGACGACAGGCAGGGTGACGCTGGGAGGG
TTTAATTCCAGATGTTTCTCAGGGGTGGGG
mm4__chr21 37.30 Mbp_chr21_37298149 CCGTGGTGAACTGGAGACAAATAATTGTTAAAAGGTTTTCTCTTGCCTGCCCCCCTTCCCCTCCATGGGACAAGACTATA
ACCAGAGGCTGATTAAGCCTGTGCCCATTGG
mm3__chr17 50.15 Mbp_chr17_50152207 TGCATCTCAGGCCTTTCTGCGGCCTCTGCCTCCTCCCCCGCCCTCTTCCCCTCCATCCTCTTCCTCCTTTTCCTAATCTC
CCACTCTCTCCCCCCTGGCCTGGAAGGGCTGGCAAAGGGAGCCCTCCTACCACTCCA
mm4__chr19 3.77 Mbp_chr19_3768010 CACTCATGGGCAGGGTAGCCCGAGGGCTCCTGGCCGACGTCCCCACCAGCGCAGCAAGTAGAATCAGAGGGAAGGGACTG
CCCCTTCCTTCCTGCTGCAGTCACAGCGTGGTCCTCACTGGCCTATCCCTG
mm4__chr2 100.84 Mbp_chr2_100835856 GAAGTCACTCAGGGTCACCCCAAAACATATCCTCCTGCCCCTCTTCCCCTCCACAGCTGAGCACAGACCTCAGCACTCAA
mm4__chr2 61.47 Mbp_chr2_61470321 GAAAGCCTCGTCCTCCGACCCCACCTCCCCGCACCTTCCCGGCAGGGCGAAGGAGGCGAAGGCGGCGAGGGGTTCATTAC
TGCCTTCATTAATTGACACCTGTAATGAGGAAACTTTCCGAGAGCCCAGAG
mm4__chr4 186.90 Mbp_chr4_186903195 ATTGCCTCTTCTCCTGGGGACTCCCACCCCTCTTCTCCTCCATCGCCATTCCAGAATACCACTGCCCACA
mm4__chr17 44.97 Mbp_chr17_44968832 CAGGTCTGCAGGGGACAAAGAGCGATTGAGGGGAGGACGGAGCGGGGAGCTGGAAGGTAGGATGAGAAAGGGGGTTATGA
AGCGAAGAAGAGGATACGGAGGGGACC
mm4__chr11 65.92 Mbp_chr11_65916632 TTAGACGTTCCTGGAGGGGTCAACCCCTGGAATTATGGGAAATCTCTGAGAAACTGATTACGGATGGAGGGGAAGTGGGG
CTGGTTTTGCAGAGGTTCTTAGAGACAGTGCCTAACCTCAGCAGTGAGGCACAACTT
mm4__chr17 9.54 Mbp_chr17_9544017 TGGAACCCACAGGAAAATCAGTTTCATTTTCTTCAGCTTGTTCTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNACCTCCCTGCCCCTCTGCCCCTCCACCGCAATCCCACTCTGGTTCACCTG
mm4__chr15 73.60 Mbp_chr15_73601680 TGCTCTCTAGGTCAAGGGTCATCCAATATTTTGGAAAAACCCTAAATTTTGTAAGACAACCTCCTACCCCACCCTCTTCC
CCACCATCGTCTCCACCCACTATCTTAAGTGACTGGGCCCAGATCTGCAAGAGTT

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.