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Contents:

PCR primers for off-targets of CTTAGGCCGCCAGGGGGCGC CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529733_F TCGTCGGCAGCGTCTGGTGAGCTGCGAGAATAGC 60.1 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529733_R GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT 60.6 1.00
mm4__chr10 80.17 Mbp_chr10_80169257_F TCGTCGGCAGCGTCCATAGGGAGGAACAGGCTGC 60.1 0.37
mm4__chr10 80.17 Mbp_chr10_80169257_R GTCTCGTGGGCTCGGAACATGCCCAACCTGTACCC 60.2 0.37
mm3__chr5 171.66 Mbp_chr5_171658576_F Primer3: not found at this Tm N.d. 0.37
mm3__chr5 171.66 Mbp_chr5_171658576_R Primer3: not found at this Tm N.d. 0.37
mm4__chr12 123.14 Mbp_chr12_123140158_F TCGTCGGCAGCGTCGAACCAGGTTCAGCTGCTCT 59.9 0.36
mm4__chr12 123.14 Mbp_chr12_123140158_R GTCTCGTGGGCTCGGAGGTCCCCTTAGCAGGAGTT 59.8 0.36
mm4__chr16 11.59 Mbp_chr16_11586751_F Primer3: not found at this Tm N.d. 0.35
mm4__chr16 11.59 Mbp_chr16_11586751_R Primer3: not found at this Tm N.d. 0.35
mm4__chr7 155.35 Mbp_chr7_155345043_F TCGTCGGCAGCGTCCTAGTCCCCGCCCCTTCG 61.5 0.34
mm4__chr7 155.35 Mbp_chr7_155345043_R GTCTCGTGGGCTCGGACTGCCTTGTGCCTTGAAGT 60.1 0.34
mm4__chr7 151.38 Mbp_chr7_151381403_F TCGTCGGCAGCGTCAGGACAGGAGCCACCCTAAA 60.1 0.31
mm4__chr7 151.38 Mbp_chr7_151381403_R GTCTCGTGGGCTCGGTCGCAGGCGTCTGGTTTT 59.8 0.31
mm4__chr12 45.99 Mbp_chr12_45990744_F TCGTCGGCAGCGTCTTCTGCTTACTCGCTCGCTC 60.1 0.30
mm4__chr12 45.99 Mbp_chr12_45990744_R GTCTCGTGGGCTCGGCCACGCTTCTCGGTCTCAAA 60.3 0.30
mm4__chr6 30.07 Mbp_chr6_30071579_F TCGTCGGCAGCGTCAGTCTCCACCTCCCAGCAG 60.6 0.29
mm4__chr6 30.07 Mbp_chr6_30071579_R GTCTCGTGGGCTCGGCGTACAACTGGCCCCACC 60.3 0.29
mm3__chr11 65.00 Mbp_chr11_64996959_F TCGTCGGCAGCGTCACAGAGGTGCATGGCTTAGG 59.7 0.28
mm3__chr11 65.00 Mbp_chr11_64996959_R GTCTCGTGGGCTCGGACTGAAACTTGGCCCTCTGG 59.8 0.28
mm4__chr6 109.45 Mbp_chr6_109454712_F TCGTCGGCAGCGTCTGCTGAGCATACATCCTGCC 60.1 0.27
mm4__chr6 109.45 Mbp_chr6_109454712_R GTCTCGTGGGCTCGGACTTCTCAGACACAGCGTGG 59.9 0.27
mm4__chr3 128.87 Mbp_chr3_128873267_F Primer3: not found at this Tm N.d. 0.27
mm4__chr3 128.87 Mbp_chr3_128873267_R Primer3: not found at this Tm N.d. 0.27
mm4__chr14 36.52 Mbp_chr14_36516713_F TCGTCGGCAGCGTCAAACTATTTTCGCGGAGGGC 58.9 0.26
mm4__chr14 36.52 Mbp_chr14_36516713_R GTCTCGTGGGCTCGGAGCTGGGGAGAGGAAAGAGT 59.8 0.26
mm4__chr17 8.38 Mbp_chr17_8383594_F TCGTCGGCAGCGTCGTACCTTCACTCCCAGGTGC 60.0 0.25
mm4__chr17 8.38 Mbp_chr17_8383594_R GTCTCGTGGGCTCGGGACGGTGAGTGCAGGGAC 60.0 0.25
mm4__chr15 100.92 Mbp_chr15_100919467_F Primer3: not found at this Tm N.d. 0.24
mm4__chr15 100.92 Mbp_chr15_100919467_R Primer3: not found at this Tm N.d. 0.24
mm4__chr19 50.33 Mbp_chr19_50328297_F Primer3: not found at this Tm N.d. 0.24
mm4__chr19 50.33 Mbp_chr19_50328297_R Primer3: not found at this Tm N.d. 0.24
mm4__chr4 56.54 Mbp_chr4_56544703_F TCGTCGGCAGCGTCCAACTTGTGTCGCAGTTCGA 59.0 0.23
mm4__chr4 56.54 Mbp_chr4_56544703_R GTCTCGTGGGCTCGGCCAGGTGGCGCTCCAGAC 62.7 0.23
mm4__chr17 42.10 Mbp_chr17_42098652_F TCGTCGGCAGCGTCCTCGAGTTCTGAAGGCTGGG 60.1 0.23
mm4__chr17 42.10 Mbp_chr17_42098652_R GTCTCGTGGGCTCGGTGTCTGAAGCGGGGAATTCC 60.0 0.23
mm3__chr19 11.54 Mbp_chr19_11536224_F TCGTCGGCAGCGTCCGAGTGAGTCATCGCGTGA 59.8 0.23
mm3__chr19 11.54 Mbp_chr19_11536224_R GTCTCGTGGGCTCGGCTACTGCGCATGTGAGGACC 60.8 0.23
mm4__chr5 108.56 Mbp_chr5_108560715_F TCGTCGGCAGCGTCACACTATTGTAATCTGCACACAGA 58.2 0.23
mm4__chr5 108.56 Mbp_chr5_108560715_R GTCTCGTGGGCTCGGTTGAACTACTCCCTCTCAATTTCA 57.5 0.23
mm4__chr22 38.93 Mbp_chr22_38927383_F TCGTCGGCAGCGTCGTGGGTGGGATTCAGGCTG 60.3 0.22
mm4__chr22 38.93 Mbp_chr22_38927383_R GTCTCGTGGGCTCGGGTGCTGCCATCTGCTCTGG 61.1 0.22
mm4__chr22 21.55 Mbp_chr22_21551063_F TCGTCGGCAGCGTCGGTCTCCAGACTTCGCCTG 59.7 0.22
mm4__chr22 21.55 Mbp_chr22_21551063_R GTCTCGTGGGCTCGGCAAGAGGCGGAAGGGGCA 62.0 0.22
mm4__chr22 21.38 Mbp_chr22_21383748_F TCGTCGGCAGCGTCCAAGAGGCGGAAGGGGCA 62.0 0.22
mm4__chr22 21.38 Mbp_chr22_21383748_R GTCTCGTGGGCTCGGGGTCTCCAGACTTCGCCTG 59.7 0.22
mm4__chr22 18.61 Mbp_chr22_18611521_F TCGTCGGCAGCGTCGGTCTCCAGACTTCGCCTG 59.7 0.22
mm4__chr22 18.61 Mbp_chr22_18611521_R GTCTCGTGGGCTCGGCAAGAGGCGGAAGGGGCA 62.0 0.22
mm4__chr11 61.76 Mbp_chr11_61758135_F TCGTCGGCAGCGTCCTGGGTGGGGAGTACAGTTG 59.6 0.22
mm4__chr11 61.76 Mbp_chr11_61758135_R GTCTCGTGGGCTCGGACTTCCCTCACTGATGCGAC 59.7 0.22
mm4__chr7 101.17 Mbp_chr7_101172401_F Primer3: not found at this Tm N.d. 0.22
mm4__chr7 101.17 Mbp_chr7_101172401_R Primer3: not found at this Tm N.d. 0.22
mm3__chr17 28.35 Mbp_chr17_28347145_F TCGTCGGCAGCGTCGGCTGAGTTCCTGTGCTTCT 59.9 0.19
mm3__chr17 28.35 Mbp_chr17_28347145_R GTCTCGTGGGCTCGGTCACAGTTCCAGCCCTTGTT 59.4 0.19
mm4__chr18 50.27 Mbp_chr18_50268610_F TCGTCGGCAGCGTCGGGAACTACGGGCTTTCTCG 60.4 0.19
mm4__chr18 50.27 Mbp_chr18_50268610_R GTCTCGTGGGCTCGGATTCACTTACCTAGGCCGCG 59.8 0.19
mm4__chr19 55.69 Mbp_chr19_55685656_F TCGTCGGCAGCGTCAGACCAAGGAAAAGCTGGCA 59.8 0.19
mm4__chr19 55.69 Mbp_chr19_55685656_R GTCTCGTGGGCTCGGCTGGGAGAGAAAGCAGTGGG 60.0 0.19
mm4__chr1 1.20 Mbp_chr1_1199824_F Primer3: not found at this Tm N.d. 0.18
mm4__chr1 1.20 Mbp_chr1_1199824_R Primer3: not found at this Tm N.d. 0.18
mm4__chr11 69.01 Mbp_chr11_69013742_F TCGTCGGCAGCGTCGAACTGGCAGAGGTGGTCAG 60.3 0.17
mm4__chr11 69.01 Mbp_chr11_69013742_R GTCTCGTGGGCTCGGGGGCTGGGGAAAAGGGAATT 60.2 0.17
mm4__chr1 206.91 Mbp_chr1_206907662_F TCGTCGGCAGCGTCTTTTCTTTTCCCAGGCGGCT 60.4 0.17
mm4__chr1 206.91 Mbp_chr1_206907662_R GTCTCGTGGGCTCGGCGTACGACAACCACCTTCCA 59.9 0.17
mm3__chr2 22.90 Mbp_chr2_22904286_F Primer3: not found at this Tm N.d. 0.17
mm3__chr2 22.90 Mbp_chr2_22904286_R Primer3: not found at this Tm N.d. 0.17
mm4__chr2 94.96 Mbp_chr2_94964375_F TCGTCGGCAGCGTCGTGATTCTGTGTCCCCTGCT 59.6 0.16
mm4__chr2 94.96 Mbp_chr2_94964375_R GTCTCGTGGGCTCGGTAAATGGCGCTCAGGACACC 60.3 0.16
mm4__chr4 141.13 Mbp_chr4_141133037_F TCGTCGGCAGCGTCGCGGTGGTTGCATTTTGCTT 60.8 0.15
mm4__chr4 141.13 Mbp_chr4_141133037_R GTCTCGTGGGCTCGGCGGCCACCCTTATGAGAGAA 59.1 0.15
mm2__chr12 53.32 Mbp_chr12_53321675_F TCGTCGGCAGCGTCAAAGAAGGACGAGTACGCGG 60.1 0.15
mm2__chr12 53.32 Mbp_chr12_53321675_R GTCTCGTGGGCTCGGCACTCCAGCCTGGTAACCAA 59.6 0.15
mm4__chr5 133.55 Mbp_chr5_133553100_F TCGTCGGCAGCGTCCTTGCCATCAGGTCTCTGGG 60.1 0.14
mm4__chr5 133.55 Mbp_chr5_133553100_R GTCTCGTGGGCTCGGAGTCTTGGACAAGCCTAGGC 59.3 0.14
mm4__chr10 97.32 Mbp_chr10_97319765_F TCGTCGGCAGCGTCGTTGGCGGAGACTGTGGG 60.3 0.14
mm4__chr10 97.32 Mbp_chr10_97319765_R GTCTCGTGGGCTCGGGAGCTCGGCCACGCAGTA 62.1 0.14
mm4__chr1 966.55 Kbp_chr1_966553_F TCGTCGGCAGCGTCCTGTACGACTCTGGCCATGG 60.1 0.14
mm4__chr1 966.55 Kbp_chr1_966553_R GTCTCGTGGGCTCGGACCTGTTTCCCTTCAGCGAG 59.9 0.14
mm4__chr12 70.37 Mbp_chr12_70366412_F TCGTCGGCAGCGTCGGAGACAGAGAGACACCCGA 60.3 0.14
mm4__chr12 70.37 Mbp_chr12_70366412_R GTCTCGTGGGCTCGGCTTCAGAGCCTCCTGCACC 60.0 0.14
mm4__chr1 154.76 Mbp_chr1_154760589_F TCGTCGGCAGCGTCCGAGAGGAAACCTGAACGCT 60.0 0.14
mm4__chr1 154.76 Mbp_chr1_154760589_R GTCTCGTGGGCTCGGCAGGCGGTCGTGGAGAGG 62.1 0.14
mm4__chr20 31.86 Mbp_chr20_31860938_F TCGTCGGCAGCGTCTAGAGAAGGAGGTGGGGTCC 59.6 0.13
mm4__chr20 31.86 Mbp_chr20_31860938_R GTCTCGTGGGCTCGGAAAAGGGGATGCGAGGGAG 59.3 0.13
mm4__chr2 75.03 Mbp_chr2_75028069_F TCGTCGGCAGCGTCCATACCTGCTGCCCACAGTC 60.7 0.13
mm4__chr2 75.03 Mbp_chr2_75028069_R GTCTCGTGGGCTCGGTCTACCCACTGCTGTCCTCT 59.5 0.13
mm4__chr5 92.91 Mbp_chr5_92909890_F Primer3: not found at this Tm N.d. 0.13
mm4__chr5 92.91 Mbp_chr5_92909890_R Primer3: not found at this Tm N.d. 0.13
mm4__chr15 63.94 Mbp_chr15_63941534_F Primer3: not found at this Tm N.d. 0.13
mm4__chr15 63.94 Mbp_chr15_63941534_R Primer3: not found at this Tm N.d. 0.13
mm4__chr22 49.64 Mbp_chr22_49640374_F TCGTCGGCAGCGTCCCATAGAAGAGGCACAGGCA 59.4 0.13
mm4__chr22 49.64 Mbp_chr22_49640374_R GTCTCGTGGGCTCGGTCTCCACGCTGTGTCCTAGA 59.9 0.13
mm4__chr16 5.08 Mbp_chr16_5077707_F TCGTCGGCAGCGTCAGGTGGTCTTTGGTTTGGGG 60.1 0.12
mm4__chr16 5.08 Mbp_chr16_5077707_R GTCTCGTGGGCTCGGTGGGAATGCACAGAGAGACG 59.7 0.12
mm4__chr9 67.22 Mbp_chr9_67217295_F Primer3: not found at this Tm N.d. 0.12
mm4__chr9 67.22 Mbp_chr9_67217295_R Primer3: not found at this Tm N.d. 0.12
mm4__chr9 61.11 Mbp_chr9_61113828_F Primer3: not found at this Tm N.d. 0.12
mm4__chr9 61.11 Mbp_chr9_61113828_R Primer3: not found at this Tm N.d. 0.12
mm4__chr9 60.84 Mbp_chr9_60838271_F Primer3: not found at this Tm N.d. 0.12
mm4__chr9 60.84 Mbp_chr9_60838271_R Primer3: not found at this Tm N.d. 0.12
mm4__chr9 41.97 Mbp_chr9_41970119_F Primer3: not found at this Tm N.d. 0.12
mm4__chr9 41.97 Mbp_chr9_41970119_R Primer3: not found at this Tm N.d. 0.12
mm4__chr9 39.15 Mbp_chr9_39149851_F Primer3: not found at this Tm N.d. 0.12
mm4__chr9 39.15 Mbp_chr9_39149851_R Primer3: not found at this Tm N.d. 0.12
mm4__chrX 112.13 Mbp_chrX_112132876_F TCGTCGGCAGCGTCGTTCCATACTTCCAACATGGCA 58.9 0.12
mm4__chrX 112.13 Mbp_chrX_112132876_R GTCTCGTGGGCTCGGTAAGAGTGGGCTAGCCTCCT 59.3 0.12
mm4__chr2 2.40 Mbp_chr2_2401497_F TCGTCGGCAGCGTCGGAAGCAACCACAACACAGC 60.2 0.12
mm4__chr2 2.40 Mbp_chr2_2401497_R GTCTCGTGGGCTCGGTCTGTGGTTTGGAGGTCAGC 59.8 0.12
mm4__chr4 21.95 Mbp_chr4_21948806_F Primer3: not found at this Tm N.d. 0.11
mm4__chr4 21.95 Mbp_chr4_21948806_R Primer3: not found at this Tm N.d. 0.11
mm4__chr17 27.35 Mbp_chr17_27353936_F TCGTCGGCAGCGTCCTCCTCCCAGCCCCTCTC 60.4 0.11
mm4__chr17 27.35 Mbp_chr17_27353936_R GTCTCGTGGGCTCGGTCTCTGCCCCTCCCTCTTC 59.9 0.11
mm4__chr9 114.21 Mbp_chr9_114212058_F TCGTCGGCAGCGTCTGGGCATGTTAGCTGTTGGA 59.5 0.11
mm4__chr9 114.21 Mbp_chr9_114212058_R GTCTCGTGGGCTCGGACAGAGGGGTCTAGGTTGGG 60.2 0.11
mm3__chr12 122.05 Mbp_chr12_122054485_F TCGTCGGCAGCGTCCAGCCATGATTGTTGGTGCG 60.4 0.11
mm3__chr12 122.05 Mbp_chr12_122054485_R GTCTCGTGGGCTCGGCTCCTGCACACTTAGCTGCT 60.0 0.11
mm4__chr8 22.55 Mbp_chr8_22551973_F TCGTCGGCAGCGTCTTCTCCTTGCCCTTCCTCCT 60.1 0.11
mm4__chr8 22.55 Mbp_chr8_22551973_R GTCTCGTGGGCTCGGCTTGCCCGGACCTACCTTC 59.7 0.11
mm4__chr14 103.71 Mbp_chr14_103706731_F Primer3: not found at this Tm N.d. 0.10
mm4__chr14 103.71 Mbp_chr14_103706731_R Primer3: not found at this Tm N.d. 0.10
mm3__chr2 190.37 Mbp_chr2_190372560_F Primer3: not found at this Tm N.d. 0.10
mm3__chr2 190.37 Mbp_chr2_190372560_R Primer3: not found at this Tm N.d. 0.10
mm4__chr8 142.45 Mbp_chr8_142445209_F Primer3: not found at this Tm N.d. 0.10
mm4__chr8 142.45 Mbp_chr8_142445209_R Primer3: not found at this Tm N.d. 0.10
mm4__chr19 3.03 Mbp_chr19_3029735_F Primer3: not found at this Tm N.d. 0.10
mm4__chr19 3.03 Mbp_chr19_3029735_R Primer3: not found at this Tm N.d. 0.10
mm4__chr9 16.42 Mbp_chr9_16419241_F TCGTCGGCAGCGTCCCACCATTGCTCCCAGACAA 60.2 0.10
mm4__chr9 16.42 Mbp_chr9_16419241_R GTCTCGTGGGCTCGGTCTCGATGACATTGACGGGG 59.5 0.10
mm4__chr4 107.93 Mbp_chr4_107932225_F TCGTCGGCAGCGTCGATGAGTCTCCAGGTGCGTG 60.4 0.09
mm4__chr4 107.93 Mbp_chr4_107932225_R GTCTCGTGGGCTCGGGCCACCACCATCACCTGTTA 59.9 0.09
mm4__chr7 55.74 Mbp_chr7_55744910_F TCGTCGGCAGCGTCAGGAACTTTTGCACCCGGAG 60.5 0.09
mm4__chr7 55.74 Mbp_chr7_55744910_R GTCTCGTGGGCTCGGGCCCAGACTGTCGTCAAGTC 60.6 0.09
mm4__chr7 39.79 Mbp_chr7_39792101_F TCGTCGGCAGCGTCACTGTCATCAAGTCGGCCTG 60.0 0.09
mm4__chr7 39.79 Mbp_chr7_39792101_R GTCTCGTGGGCTCGGGGAACTTTTGCACCCGGAGA 60.5 0.09
mm3__chr22 50.29 Mbp_chr22_50291161_F TCGTCGGCAGCGTCATAGCCCAGCAAGTGCACAT 60.0 0.09
mm3__chr22 50.29 Mbp_chr22_50291161_R GTCTCGTGGGCTCGGTGCTGGCTTCCACTGTTTCA 60.1 0.09
mm3__chr12 120.58 Mbp_chr12_120583560_F TCGTCGGCAGCGTCCACTCCCCACCCAAGAACAA 59.8 0.09
mm3__chr12 120.58 Mbp_chr12_120583560_R GTCTCGTGGGCTCGGGGGTGTCTTGCTCTCTCCG 59.7 0.09
mm4__chr11 61.75 Mbp_chr11_61752527_F Primer3: not found at this Tm N.d. 0.08
mm4__chr11 61.75 Mbp_chr11_61752527_R Primer3: not found at this Tm N.d. 0.08
mm3__chr9 137.02 Mbp_chr9_137023711_F TCGTCGGCAGCGTCACCAGCTGTGCCTTTAGGTG 60.2 0.08
mm3__chr9 137.02 Mbp_chr9_137023711_R GTCTCGTGGGCTCGGCCTCCCCGCTTGCATTAACA 60.6 0.08
mm4__chr11 68.02 Mbp_chr11_68018651_F TCGTCGGCAGCGTCCCCAAGAACCTGGTGAGCC 60.3 0.08
mm4__chr11 68.02 Mbp_chr11_68018651_R GTCTCGTGGGCTCGGGGGAGCCGGGGATGAAATTA 59.5 0.08
mm3__chr11 66.28 Mbp_chr11_66282637_F Primer3: not found at this Tm N.d. 0.08
mm3__chr11 66.28 Mbp_chr11_66282637_R Primer3: not found at this Tm N.d. 0.08
mm4__chrX 153.64 Mbp_chrX_153644229_F Primer3: not found at this Tm N.d. 0.08
mm4__chrX 153.64 Mbp_chrX_153644229_R Primer3: not found at this Tm N.d. 0.08
mm4__chr20 23.42 Mbp_chr20_23421118_F Primer3: not found at this Tm N.d. 0.07
mm4__chr20 23.42 Mbp_chr20_23421118_R Primer3: not found at this Tm N.d. 0.07
mm4__chr18 77.04 Mbp_chr18_77043696_F TCGTCGGCAGCGTCAAGCTTTGAACTTGCGTGCG 60.5 0.07
mm4__chr18 77.04 Mbp_chr18_77043696_R GTCTCGTGGGCTCGGCGTGTCCTGCATCCCTTTCT 60.0 0.07
mm4__chr14 101.96 Mbp_chr14_101964463_F Primer3: not found at this Tm N.d. 0.07
mm4__chr14 101.96 Mbp_chr14_101964463_R Primer3: not found at this Tm N.d. 0.07
mm4__chr6 105.14 Mbp_chr6_105137237_F Primer3: not found at this Tm N.d. 0.07
mm4__chr6 105.14 Mbp_chr6_105137237_R Primer3: not found at this Tm N.d. 0.07
mm4__chr19 13.85 Mbp_chr19_13850683_F Primer3: not found at this Tm N.d. 0.07
mm4__chr19 13.85 Mbp_chr19_13850683_R Primer3: not found at this Tm N.d. 0.07
mm4__chr11 842.37 Kbp_chr11_842365_F Primer3: not found at this Tm N.d. 0.07
mm4__chr11 842.37 Kbp_chr11_842365_R Primer3: not found at this Tm N.d. 0.07
mm4__chr8 60.91 Mbp_chr8_60910296_F TCGTCGGCAGCGTCGTCCCTCCAGGCTGAGCG 62.1 0.07
mm4__chr8 60.91 Mbp_chr8_60910296_R GTCTCGTGGGCTCGGGGGCGACACTTGTCCATGA 60.0 0.07
mm4__chr1 202.57 Mbp_chr1_202571917_F TCGTCGGCAGCGTCATCAAAGCCCAATGCCAGGT 60.2 0.07
mm4__chr1 202.57 Mbp_chr1_202571917_R GTCTCGTGGGCTCGGACTGGTGAGAATGCAGAGGC 60.0 0.07
mm3__chr22 41.70 Mbp_chr22_41697267_F TCGTCGGCAGCGTCAGAGGAACAGACCGCACATG 60.0 0.06
mm3__chr22 41.70 Mbp_chr22_41697267_R GTCTCGTGGGCTCGGGGGAGCTTGGTCCTAGCAAG 60.1 0.06
mm4__chr1 65.25 Mbp_chr1_65254626_F TCGTCGGCAGCGTCGGAAGCTGTTTTCCTTGCGG 60.0 0.06
mm4__chr1 65.25 Mbp_chr1_65254626_R GTCTCGTGGGCTCGGCTGCCTCAGGGTAACAGGTC 59.7 0.06
mm4__chr19 50.48 Mbp_chr19_50480802_F Primer3: not found at this Tm N.d. 0.06
mm4__chr19 50.48 Mbp_chr19_50480802_R Primer3: not found at this Tm N.d. 0.06
mm4__chr6 142.82 Mbp_chr6_142823096_F Primer3: not found at this Tm N.d. 0.06
mm4__chr6 142.82 Mbp_chr6_142823096_R Primer3: not found at this Tm N.d. 0.06
mm3__chr19 19.18 Mbp_chr19_19175211_F TCGTCGGCAGCGTCGCAGACAATGACCGCTAGGA 59.8 0.06
mm3__chr19 19.18 Mbp_chr19_19175211_R GTCTCGTGGGCTCGGGGGCGCTGGTTAATTTGCAT 59.8 0.06
mm4__chr1 148.32 Mbp_chr1_148317688_F Primer3: not found at this Tm N.d. 0.06
mm4__chr1 148.32 Mbp_chr1_148317688_R Primer3: not found at this Tm N.d. 0.06
mm4__chr1 146.39 Mbp_chr1_146393928_F Primer3: not found at this Tm N.d. 0.06
mm4__chr1 146.39 Mbp_chr1_146393928_R Primer3: not found at this Tm N.d. 0.06
mm4__chr1 144.54 Mbp_chr1_144539966_F Primer3: not found at this Tm N.d. 0.06
mm4__chr1 144.54 Mbp_chr1_144539966_R Primer3: not found at this Tm N.d. 0.06
mm3__chr2 73.98 Mbp_chr2_73984471_F TCGTCGGCAGCGTCCCATTGTCCTGCCTCGGAAT 60.1 0.06
mm3__chr2 73.98 Mbp_chr2_73984471_R GTCTCGTGGGCTCGGCACTTCCTGCCACTCCCTG 60.0 0.06
mm3__chr2 71.94 Mbp_chr2_71944373_F TCGTCGGCAGCGTCGGCTCAGACAAGCAGGGATT 60.0 0.06
mm3__chr2 71.94 Mbp_chr2_71944373_R GTCTCGTGGGCTCGGCTGAGCTTTCCTGATGCCCA 60.0 0.06
mm3__chr3 98.02 Mbp_chr3_98023410_F TCGTCGGCAGCGTCAGAGCTTCCAACTGTGGCTC 59.9 0.05
mm3__chr3 98.02 Mbp_chr3_98023410_R GTCTCGTGGGCTCGGTACATTTGCCTGTCCCTGGG 59.6 0.05
mm4__chr3 127.46 Mbp_chr3_127455716_F Primer3: not found at this Tm N.d. 0.05
mm4__chr3 127.46 Mbp_chr3_127455716_R Primer3: not found at this Tm N.d. 0.05
mm4__chr2 144.44 Mbp_chr2_144441306_F TCGTCGGCAGCGTCCCCATGCCTCAATTCCCCTT 60.0 0.05
mm4__chr2 144.44 Mbp_chr2_144441306_R GTCTCGTGGGCTCGGTCTGACTTTTGCCACGTCCA 59.8 0.05
mm3__chr1 244.37 Mbp_chr1_244367648_F TCGTCGGCAGCGTCCAGAGAGCAGAAGACAGCCC 60.1 0.05
mm3__chr1 244.37 Mbp_chr1_244367648_R GTCTCGTGGGCTCGGCTCCTGCCTAGTGCTCTGTG 59.8 0.05
mm4__chr19 3.39 Mbp_chr19_3387932_F Primer3: not found at this Tm N.d. 0.05
mm4__chr19 3.39 Mbp_chr19_3387932_R Primer3: not found at this Tm N.d. 0.05
mm4__chr18 26.66 Mbp_chr18_26656184_F TCGTCGGCAGCGTCCAAACTGAATCCCTCCCGCC 61.0 0.05
mm4__chr18 26.66 Mbp_chr18_26656184_R GTCTCGTGGGCTCGGCAAGTCAGGAGCCCGCATC 60.8 0.05
mm3__chr6 107.85 Mbp_chr6_107848398_F TCGTCGGCAGCGTCCAAGAAGAGGCAGCGGATCT 59.8 0.05
mm3__chr6 107.85 Mbp_chr6_107848398_R GTCTCGTGGGCTCGGCAGCAGGAGAAATGAGCGGG 61.0 0.05
mm4__chr17 80.11 Mbp_chr17_80112207_F TCGTCGGCAGCGTCCTCAGGGAGGAGGAAAAGCG 60.1 0.05
mm4__chr17 80.11 Mbp_chr17_80112207_R GTCTCGTGGGCTCGGCAGGCATGAAGTGGGAAGGA 59.6 0.05
mm4__chr17 9.00 Mbp_chr17_8998193_F TCGTCGGCAGCGTCCAGCATTTACTGTGCGAGCC 59.9 0.05
mm4__chr17 9.00 Mbp_chr17_8998193_R GTCTCGTGGGCTCGGTGAAGGTGCCTCAACCCATC 59.9 0.05
mm3__chr1 143.72 Mbp_chr1_143717609_F TCGTCGGCAGCGTCCTTGCCCCTAGCCTGCTTTA 59.7 0.05
mm3__chr1 143.72 Mbp_chr1_143717609_R GTCTCGTGGGCTCGGAAGAACGGGAAGAGCCATCC 59.7 0.05
mm4__chr11 117.23 Mbp_chr11_117232188_F Primer3: not found at this Tm N.d. 0.05
mm4__chr11 117.23 Mbp_chr11_117232188_R Primer3: not found at this Tm N.d. 0.05
mm2__chr2 231.71 Mbp_chr2_231710437_F Primer3: not found at this Tm N.d. 0.04
mm2__chr2 231.71 Mbp_chr2_231710437_R Primer3: not found at this Tm N.d. 0.04
mm4__chr11 62.79 Mbp_chr11_62786684_F TCGTCGGCAGCGTCTTTCAAGAGTCGTCCTCCGG 59.3 0.04
mm4__chr11 62.79 Mbp_chr11_62786684_R GTCTCGTGGGCTCGGCGAAAGAGAAGGGTCCTCCG 59.8 0.04
mm4__chr1_KI270714v1_random 30.75 Kbp_chr1_KI270714v1_random_30752_F TCGTCGGCAGCGTCCTTACATTGCCCATGCTGCC 59.8 0.04
mm4__chr1_KI270714v1_random 30.75 Kbp_chr1_KI270714v1_random_30752_R GTCTCGTGGGCTCGGCAGGGTGCCTGTGACACTT 59.8 0.04
mm4__chr1 6.49 Mbp_chr1_6490036_F Primer3: not found at this Tm N.d. 0.04
mm4__chr1 6.49 Mbp_chr1_6490036_R Primer3: not found at this Tm N.d. 0.04
mm4__chr16 89.17 Mbp_chr16_89168186_F TCGTCGGCAGCGTCATACCTGATGGGGCTCCTCT 59.4 0.04
mm4__chr16 89.17 Mbp_chr16_89168186_R GTCTCGTGGGCTCGGCATCCTGGACCCTGCTGTG 60.0 0.04
mm4__chr8 27.90 Mbp_chr8_27895281_F TCGTCGGCAGCGTCTGCAGAGTAGAGCCCTAGGG 60.1 0.04
mm4__chr8 27.90 Mbp_chr8_27895281_R GTCTCGTGGGCTCGGCAGTGTCACCTCAGCCTCAG 60.0 0.04
mm4__chr11 2.86 Mbp_chr11_2862409_F TCGTCGGCAGCGTCCTTCGGGGTGCCCAGTCC 62.4 0.03
mm4__chr11 2.86 Mbp_chr11_2862409_R GTCTCGTGGGCTCGGTTTGTGCGCGCATTAAAGCT 60.0 0.03
mm4__chr17 45.89 Mbp_chr17_45894499_F TCGTCGGCAGCGTCCTGGGGAGGCTCGCGTTC 62.8 0.03
mm4__chr17 45.89 Mbp_chr17_45894499_R GTCTCGTGGGCTCGGCTGTTGGTGCCGGAGCTG 61.0 0.03
mm4__chr1 18.30 Mbp_chr1_18295154_F TCGTCGGCAGCGTCAGTCGGGTGGATCATCAGGA 60.0 0.03
mm4__chr1 18.30 Mbp_chr1_18295154_R GTCTCGTGGGCTCGGTGCCTCTGGGACGCTCTAT 60.0 0.03
mm4__chr7 55.74 Mbp_chr7_55741124_F TCGTCGGCAGCGTCCCAGGCCAACTTAAGGACGA 59.6 0.03
mm4__chr7 55.74 Mbp_chr7_55741124_R GTCTCGTGGGCTCGGCGACCTCTGCAGGCCTAAAT 59.8 0.03
mm4__chr7 51.39 Mbp_chr7_51386655_F Primer3: not found at this Tm N.d. 0.03
mm4__chr7 51.39 Mbp_chr7_51386655_R Primer3: not found at this Tm N.d. 0.03
mm4__chr7 39.79 Mbp_chr7_39794024_F TCGTCGGCAGCGTCGGCCCAAATCGTCCTGAAGT 60.3 0.03
mm4__chr7 39.79 Mbp_chr7_39794024_R GTCTCGTGGGCTCGGAGCTCTTCCAGGCCCATTTC 60.0 0.03
mm4__chr19 46.72 Mbp_chr19_46716290_F Primer3: not found at this Tm N.d. 0.03
mm4__chr19 46.72 Mbp_chr19_46716290_R Primer3: not found at this Tm N.d. 0.03
mm4__chr1 169.01 Mbp_chr1_169013744_F TCGTCGGCAGCGTCTCATTGTGTTGCCTCCAAGC 59.0 0.03
mm4__chr1 169.01 Mbp_chr1_169013744_R GTCTCGTGGGCTCGGCCCTCACCATTTCTTCAGGCT 59.9 0.03
mm4__chr5 73.12 Mbp_chr5_73120216_F TCGTCGGCAGCGTCCTCGCGCCCCTATAGAACTG 60.0 0.03
mm4__chr5 73.12 Mbp_chr5_73120216_R GTCTCGTGGGCTCGGGGCGAGTTGAGATGCCCC 60.5 0.03
mm4__chr10 100.01 Mbp_chr10_100010067_F Primer3: not found at this Tm N.d. 0.03
mm4__chr10 100.01 Mbp_chr10_100010067_R Primer3: not found at this Tm N.d. 0.03
mm4__chr15 28.01 Mbp_chr15_28012980_F Primer3: not found at this Tm N.d. 0.03
mm4__chr15 28.01 Mbp_chr15_28012980_R Primer3: not found at this Tm N.d. 0.03
mm4__chr14 68.63 Mbp_chr14_68628296_F TCGTCGGCAGCGTCCTAAACATGCAGCAGCCAGC 60.1 0.03
mm4__chr14 68.63 Mbp_chr14_68628296_R GTCTCGTGGGCTCGGCTGCGTTAACCCCTTCCCAT 60.0 0.03
mm4__chr16 785.77 Kbp_chr16_785771_F TCGTCGGCAGCGTCGTCGTCACCTGTGATCACGG 60.7 0.02
mm4__chr16 785.77 Kbp_chr16_785771_R GTCTCGTGGGCTCGGGTCTGCTCACTGGCTCTGG 60.0 0.02
mm4__chr16 66.61 Mbp_chr16_66608706_F TCGTCGGCAGCGTCTGCCTCTAGAGCAGGTCTGT 59.9 0.02
mm4__chr16 66.61 Mbp_chr16_66608706_R GTCTCGTGGGCTCGGCAGCTCATTCCTGTAGGCCC 60.1 0.02
mm4__chr22 41.92 Mbp_chr22_41919575_F TCGTCGGCAGCGTCGGCAACTCTGACTCAGGCTC 60.3 0.02
mm4__chr22 41.92 Mbp_chr22_41919575_R GTCTCGTGGGCTCGGACGTACACTGAGGGCTTTGG 59.9 0.02
mm4__chr21 42.19 Mbp_chr21_42187049_F Primer3: not found at this Tm N.d. 0.02
mm4__chr21 42.19 Mbp_chr21_42187049_R Primer3: not found at this Tm N.d. 0.02
mm4__chr4 139.30 Mbp_chr4_139295839_F TCGTCGGCAGCGTCCTCAGTCTCTCCGAGTTGGC 59.8 0.02
mm4__chr4 139.30 Mbp_chr4_139295839_R GTCTCGTGGGCTCGGGTGCTAGAGAACCTTGCCGT 60.0 0.02
mm4__chr14 98.96 Mbp_chr14_98957804_F Primer3: not found at this Tm N.d. 0.02
mm4__chr14 98.96 Mbp_chr14_98957804_R Primer3: not found at this Tm N.d. 0.02
mm3__chr22 22.86 Mbp_chr22_22855863_F TCGTCGGCAGCGTCAGCTATGACTCAGGGGAGGT 59.3 0.01
mm3__chr22 22.86 Mbp_chr22_22855863_R GTCTCGTGGGCTCGGAGGAGCTCCTAGGACCCAC 59.6 0.01
mm4__chr16 2.19 Mbp_chr16_2190395_F TCGTCGGCAGCGTCAGGAGACTCAGTGAGGGGAG 59.6 0.01
mm4__chr16 2.19 Mbp_chr16_2190395_R GTCTCGTGGGCTCGGCGAGTGGACGAGTGAGTGTC 60.1 0.01
mm4__chr13 85.70 Mbp_chr13_85696535_F Primer3: not found at this Tm N.d. 0.01
mm4__chr13 85.70 Mbp_chr13_85696535_R Primer3: not found at this Tm N.d. 0.01
mm4__chr20 59.93 Mbp_chr20_59934161_F TCGTCGGCAGCGTCACTCCTGGGTGAGGCCTG 60.6 0.01
mm4__chr20 59.93 Mbp_chr20_59934161_R GTCTCGTGGGCTCGGGCCTAGGAATACACAGTTGGGT 59.7 0.01
mm4__chr7 53.35 Mbp_chr7_53350431_F Primer3: not found at this Tm N.d. 0.01
mm4__chr7 53.35 Mbp_chr7_53350431_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 201.11 Mbp_chr1_201112460_F TCGTCGGCAGCGTCTGCTTTCCCTTCCCTGTGTC 59.8 0.01
mm4__chr1 201.11 Mbp_chr1_201112460_R GTCTCGTGGGCTCGGGGGAGCGAGGAGAGTAATCC 59.0 0.01
mm4__chr7 75.42 Mbp_chr7_75418655_F TCGTCGGCAGCGTCGGCTTGTGCTTCCTCCAGAA 60.2 0.01
mm4__chr7 75.42 Mbp_chr7_75418655_R GTCTCGTGGGCTCGGGCAAACCTACCCCGGATCTC 60.1 0.01
mm4__chr7 73.30 Mbp_chr7_73301281_F TCGTCGGCAGCGTCACCTTGGCACGTGCTAGAAA 59.8 0.01
mm4__chr7 73.30 Mbp_chr7_73301281_R GTCTCGTGGGCTCGGGGCTTGTGCTTCCTCCAGAA 60.2 0.01
mm4__chr7 72.95 Mbp_chr7_72946351_F TCGTCGGCAGCGTCGAAGAGCCTTGGACCCTTCC 60.0 0.01
mm4__chr7 72.95 Mbp_chr7_72946351_R GTCTCGTGGGCTCGGGGCTTGTGCTTCCTCCAGAA 60.2 0.01
mm4__chr8 52.82 Mbp_chr8_52815209_F Primer3: not found at this Tm N.d. 0.01
mm4__chr8 52.82 Mbp_chr8_52815209_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 31.83 Mbp_chr1_31830856_F Primer3: not found at this Tm N.d. 0.01
mm4__chr1 31.83 Mbp_chr1_31830856_R Primer3: not found at this Tm N.d. 0.01
mm4__chr7 128.27 Mbp_chr7_128267848_F TCGTCGGCAGCGTCATCCCTTGCTGGAGGCTAGA 60.0 0.00
mm4__chr7 128.27 Mbp_chr7_128267848_R GTCTCGTGGGCTCGGCATTTGCACTCCTCCTCCCA 59.6 0.00
mm4__chr6 73.16 Mbp_chr6_73157416_F TCGTCGGCAGCGTCGAGTCCACTGTGTCAGGCTT 59.6 0.00
mm4__chr6 73.16 Mbp_chr6_73157416_R GTCTCGTGGGCTCGGTCTGGGAATCTGTGGTGGTG 59.3 0.00
mm4__chr1 204.69 Mbp_chr1_204692988_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 204.69 Mbp_chr1_204692988_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 168.40 Mbp_chr6_168397179_F TCGTCGGCAGCGTCGCAGACAGAGCTCCAGAAGG 60.1 0.00
mm4__chr6 168.40 Mbp_chr6_168397179_R GTCTCGTGGGCTCGGATTTGCTGGAAGGGTCCCTG 59.9 0.00
mm4__chr5 140.07 Mbp_chr5_140074389_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 140.07 Mbp_chr5_140074389_R Primer3: not found at this Tm N.d. 0.00
mm4__chr17 76.14 Mbp_chr17_76137927_F TCGTCGGCAGCGTCCTGGGCCTCGGTATTCACC 59.8 0.00
mm4__chr17 76.14 Mbp_chr17_76137927_R GTCTCGTGGGCTCGGCTTTCAAGAAGCGGCGACTG 59.8 0.00
mm4__chr16 88.94 Mbp_chr16_88936184_F TCGTCGGCAGCGTCTACTCAGGGAGGTCGGGTAC 59.7 0.00
mm4__chr16 88.94 Mbp_chr16_88936184_R GTCTCGTGGGCTCGGTGGGAGATACCTGGGAGACG 60.1 0.00
mm4__chr16 30.59 Mbp_chr16_30585406_F TCGTCGGCAGCGTCCTTCCAGCCCACTAGCCTCT 60.9 0.00
mm4__chr16 30.59 Mbp_chr16_30585406_R GTCTCGTGGGCTCGGGGACGTGATGCGGGAGAC 60.2 0.00
mm4__chr12 113.29 Mbp_chr12_113286415_F TCGTCGGCAGCGTCAAGTCAGGCTGCACTCATGA 59.3 0.00
mm4__chr12 113.29 Mbp_chr12_113286415_R GTCTCGTGGGCTCGGGGACCCTCTCTCCTCTCCAG 60.1 0.00
mm4__chr10 120.46 Mbp_chr10_120461540_F TCGTCGGCAGCGTCGTGGCTCTCTGTCTGCAGG 60.0 0.00
mm4__chr10 120.46 Mbp_chr10_120461540_R GTCTCGTGGGCTCGGGCAAAGGCCTCTTGCATCCT 60.9 0.00
mm3__chr14 69.73 Mbp_chr14_69726929_F TCGTCGGCAGCGTCCTGCAGCAGAGGCCGGAG 62.8 0.00
mm3__chr14 69.73 Mbp_chr14_69726929_R GTCTCGTGGGCTCGGTTCTTCCCAGGGAAAGTGGC 59.8 0.00
mm3__chr13 110.21 Mbp_chr13_110205351_F TCGTCGGCAGCGTCGCTCACAATAGTGCCAGCGT 61.0 0.00
mm3__chr13 110.21 Mbp_chr13_110205351_R GTCTCGTGGGCTCGGGCCTCTGACGGTTACTGCTT 60.0 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529733 TGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT
AGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG
mm4__chr10 80.17 Mbp_chr10_80169257 CATAGGGAGGAACAGGCTGCTGGGCAGAGGGGGGCTGCCCAAAGCCGCCAGGGGGCACTGGTGGGTAGCCAGCCCCAGGG
GCCCCAGGGTACAGGTTGGGCATGTT
mm4__chr12 123.14 Mbp_chr12_123140158 GAACCAGGTTCAGCTGCTCTGAACATGCCCCCTTAGGCCCCCGGGGAACGCAGGCCCTATAACTCCTGCTAAGGGGACCT
mm4__chr7 155.35 Mbp_chr7_155345043 CTAGTCCCCGCCCCTTCGCCCCCGCCGCGCCGCACCAGCGCCCCCTGGCGGCCATCCGCGCGCCGCGCGCTCCGACTCCC
GCCCACCCTAGAGAGAGCGCGGAGCCCTGCCGGCGACTGACTTCAAGGCACAAGGCAGT
mm4__chr7 151.38 Mbp_chr7_151381403 AGGACAGGAGCCACCCTAAAAGGGAAAAGGGGGCGGTCCCCAGGGCGAGCACTCCCGCTCCCAGCGCCTCCTGGCCACCA
AAGAAAAACCAGACGCCTGCGA
mm4__chr12 45.99 Mbp_chr12_45990744 TTCTGCTTACTCGCTCGCTCTGGCCCTGAGTGCATGTTCGTAGTACCCAGTTGGGCCGCAGGGGGGCGCTGGCAAACTGC
TGTTTGAGACCGAGAAGCGTGG
mm4__chr6 30.07 Mbp_chr6_30071579 AGTCTCCACCTCCCAGCAGTGGCGGCCGGCCCCGAAGCCCTGCGCACCCAGCACAGCTGGGAGCTGATCGAAGCGCTTGG
GGCCGTCAGGGGGCGCGGGCGTCCCTGGTGGGGCCAGTTGTACG
mm3__chr11 65.00 Mbp_chr11_64996959 ACAGAGGTGCATGGCTTAGGCGGGGGAGCAGAGTGGTCCCTCAGCAGCTGTGACTTCCCAGTGCCCTCTGGAGGCCTAAG
GAAGTGGCAGCTCTTTAAATCCCCAGTGGCCACTCCGCCCCCAGAGGGCCAAGTTTCAGT
mm4__chr6 109.45 Mbp_chr6_109454712 TGCTGAGCATACATCCTGCCCCATCTCCCTCCAAGCGTCCCTGTGCCGGCGCCCCTTGATGGCCTCAGTGCCACGCTGTG
TCTGAGAAGT
mm4__chr14 36.52 Mbp_chr14_36516713 AAACTATTTTCGCGGAGGGCGGTCGCCGCTGAGCCTAGGCGGCCAGAGGGTGCCGGGGAGGGGGCACTTCCTTTGTGTCA
GTGACAAGTGGGTTATGTTGAAGACTCTTTCCTCTCCCCAGCT
mm4__chr17 8.38 Mbp_chr17_8383594 GTACCTTCACTCCCAGGTGCCGGGGCGGGGGCCGGCACCCCTTAGCCGGGGAAGCGCAGGCCCTGCTTCTGGCCGCCAGG
AGGCGCGGGGACGCCGGCCGGCCGGTCGCCTGGGTCCCTGCACTCACCGTC
mm4__chr4 56.54 Mbp_chr4_56544703 CAACTTGTGTCGCAGTTCGAGTTGCTGGGCTCCGCTAGGCCGAGCCCCGCAGCCCTCCAGCCGCTGCCAACCTGCGCCCC
CTGGTGGCCTGGTTGCCGGCCGCACGCGCTCTGGCTCCGCCGTCTGGAGCGCCACCTGG
mm4__chr17 42.10 Mbp_chr17_42098652 CTCGAGTTCTGAAGGCTGGGAAGTTAGGACTCCAGGGGGTGCGGGGGGTGGGTGGGAATTCCCCGCTTCAGACA
mm3__chr19 11.54 Mbp_chr19_11536224 CGAGTGAGTCATCGCGTGACGTCACCGCCGCCTGGACCTCGCCAGGTCCCGCCCCCGCCCCTCCCGGGCCGCCAGGGGGC
GCCGGCTGCAGGTCCTCACATGCGCAGTAG
mm4__chr5 108.56 Mbp_chr5_108560715 ACACTATTGTAATCTGCACACAGATTTAAGACATAAAATCCCTGTGCTCCCTGGTGGCCTCAGGGGTGACATCACTTGTT
ATGAAATTGAGAGGGAGTAGTTCAA
mm4__chr22 38.93 Mbp_chr22_38927383 GTGGGTGGGATTCAGGCTGCCGGGGGCGGGGGGGGGGGTTGCCGCAGAGGGGGCTGGGGAGGGCGGCACCCCCCCATTGC
CCGTCCTGGGCCCTGCGTCCCCTGGCGGGCTGCGGCCAGAGCAGATGGCAGCAC
mm4__chr22 21.55 Mbp_chr22_21551063 GGTCTCCAGACTTCGCCTGGCAGGCCTCAGCTCGCCAACGGCCGCCGCCGACCGCCCGGCGCGCCCCTTCCGGCGCCCCC
TGGGGGTCTCCGCGTGCCCCTTCCGCCTCTTG
mm4__chr22 21.38 Mbp_chr22_21383748 CAAGAGGCGGAAGGGGCACGCGGAGACCCCCAGGGGGCGCCGGAAGGGGCGCGCCGGGCGGTCGGCGGCGGCCGTTGGCG
AGCTGAGGCCTGCCAGGCGAAGTCTGGAGACC
mm4__chr22 18.61 Mbp_chr22_18611521 GGTCTCCAGACTTCGCCTGGCAGGCCTCAGCTCGCCAACGGCCGCCGCCGACCGCCCGGCGCGCCCCTTCCGGCGCCCCC
TGGGGGTCTCCGCGTGCCCCTTCCGCCTCTTG
mm4__chr11 61.76 Mbp_chr11_61758135 CTGGGTGGGGAGTACAGTTGGGTCCGGAGCCCCCTGCCGGCCTAGAGTATGGAGGGGGCTCCCGGACATTCTGTCCTCCC
TGTGCCCCAGGTCGCATCAGTGAGGGAAGT
mm3__chr17 28.35 Mbp_chr17_28347145 GGCTGAGTTCCTGTGCTTCTGTTTATCATCTGACCTTGTGTTCTAGCCAGGTCGTAAGCGCGACTGTACTGTTGCCCTGC
AACCCCAGCTCCCCCTGGAGGACTAAGGACAAGAATAACAAGGGCTGGAACTGTGA
mm4__chr18 50.27 Mbp_chr18_50268610 GGGAACTACGGGCTTTCTCGCCCCGGCGCCCCCTGGCGGACCCACCAGCAGGTTGAAGGTGTCCGGCCAGTGCTGAGCAC
CAAGAGCCTCAGCCTTCAGCCAACCCCCCGCCCCCGCGGCCTAGGTAAGTGAAT
mm4__chr19 55.69 Mbp_chr19_55685656 AGACCAAGGAAAAGCTGGCACAGACCGCCACGGGTGAGTCCCTCCCTGCGGCCCCTGACGGCCTAGAGTCTGTCCTCCGC
CTACTGCGGCTCCCGGCACCCGGCCGCCCACTGCTTTCTCTCCCAG
mm4__chr11 69.01 Mbp_chr11_69013742 GAACTGGCAGAGGTGGTCAGCACCGCCCAAGCGGCACCCCTCCCCCTTCCCACCCCGGACTCGGCCTGGGCCCCCTGGGG
GCTTATGGAACCAGGAATTCCCTTTTCCCCAGCCC
mm4__chr1 206.91 Mbp_chr1_206907662 TTTTCTTTTCCCAGGCGGCTGCCGAAGATGGCGGGGGTGCAGGTCCTGGTGCTTGATGGTGGAGGCCATCTCCTGGGCCC
CCTGGCGGCCATCGTGGCTAACTAGGTACTGCTGGGCTGGAAGGTGGTTGTCGTACG
mm4__chr2 94.96 Mbp_chr2_94964375 GTGATTCTGTGTCCCCTGCTTGTCCTGAGCCCCCTGGTGTCCTCAGTGCCCCCTGGTGGTTCTGAGCACACCCCCTGGTG
TCTTCAGTACCCCCTGGTGTCCTGAGCGCCATTTA
mm4__chr4 141.13 Mbp_chr4_141133037 GCGGTGGTTGCATTTTGCTTCTTGGGCGCCCGGGGGGCGCGGGAACAGAGGGCCCGCGGGGCTTGCGGAGGAGTCCTGCT
GCCGAGCGTCCTGCTCCTTCGCCCGGCTTCGCCTTCTCTCATAAGGGTGGCCG
mm2__chr12 53.32 Mbp_chr12_53321675 AAAGAAGGACGAGTACGCGGTCCCGGGACAGACCTCCAAAGCTTCTTCCTGCGCCCCCTGGCGGCCGGAGGGGGAGCAGC
GCGGACACGTCCCCCGGCCAACCGTTGGTTACCAGGCTGGAGTG
mm4__chr5 133.55 Mbp_chr5_133553100 CTTGCCATCAGGTCTCTGGGCCTGGAAAACGTAACTCTCCCTCTGACCTCCAGGGGGCGCGGGAGAGAGGGTGAGTCCCG
CGGTACCCGGAACCCACAGTCTGTATCAAGAGGGAGGAAGCCTAGGCTTGTCCAAGACT
mm4__chr10 97.32 Mbp_chr10_97319765 GTTGGCGGAGACTGTGGGCCCGGCGCCCCCTGGGGTCCTGCGCTGCGCCCTGGGGGACCGCGGCCGCGTGCGGGGCCGCG
CTGCGCCCTACTGCGTGGCCGAGCTC
mm4__chr1 966.55 Kbp_chr1_966553 CTGTACGACTCTGGCCATGGGGAACAGCCACTGTGTCCCTCAGGCCCCCAGGAGGCTCCGGGCCTCCTTCTCCAGAAAGC
CCTCGCTGAAGGGAAACAGGT
mm4__chr12 70.37 Mbp_chr12_70366412 GGAGACAGAGAGACACCCGACGAGAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCG
CTGGAAGCTGGAGCGGTCCGTGCGCTCCCCGCGCCCGAGGGTGCAGGAGGCTCTGAAG
mm4__chr1 154.76 Mbp_chr1_154760589 CGAGAGGAAACCTGAACGCTCTGCTCCTCCACAGCGAGTCCCTGCAGGTGGGGACACCGCCCACCTGGCCCTCCCTGCGC
CCCCTGGCGGAGCGAGCCTCGGCCTCTCCCGGGCCCCGGCCTCTCCACGACCGCCTG
mm4__chr20 31.86 Mbp_chr20_31860938 TAGAGAAGGAGGTGGGGTCCCAGGAGGCAACTCCGCAGACTGCAGTTGCGGGAGGGTGGTGGGCTCCTCAGGCCCCCAGG
GGATGCAGGCTCCCTCGCATCCCCTTTT
mm4__chr2 75.03 Mbp_chr2_75028069 CATACCTGCTGCCCACAGTCAGGCCCCTGACCTCACACCGGCTGCCGTCACAACCTGGGGCAGCCAGGGGGCTCTGGCAC
TGCCACCAGAGACTGGTAGATGACCACCTGTGGACCCAGAGGACAGCAGTGGGTAGA
mm4__chr22 49.64 Mbp_chr22_49640374 CCATAGAAGAGGCACAGGCAGCCCCCATGGCACTGAGGCCCCCAGAGGGTGCGGGTCTCGGGAGGGGGCTCCCTCAGGCC
TGCGTGTCACCACTGGGCTGCCACCCCATCCAGCCCACCCTCTAGGACACAGCGTGGAGA
mm4__chr16 5.08 Mbp_chr16_5077707 AGGTGGTCTTTGGTTTGGGGAAGCTGGGGGAGGAGGGGGCAGAGTTTCTTTCTTAGCCAGCCAGGGGGTCCAGGCAGTTT
TGGTCCTAGGACGTCTCTCTGTGCATTCCCA
mm4__chrX 112.13 Mbp_chrX_112132876 GTTCCATACTTCCAACATGGCATTACATTCTTTATACACTCCAGTGCCCACTGGCGGTCAAAGACAAATCTTTTGCCTTG
AGTATTTGAATGTTATAGATGGCTATTACCTCCTGGAGGAGGCTAGCCCACTCTTA
mm4__chr2 2.40 Mbp_chr2_2401497 GGAAGCAACCACAACACAGCTTCTTTTCCATCCCCCGGCACCCGCTGGCAGCCTCAGCAGCTGACCTCCAAACCACAGA
mm4__chr17 27.35 Mbp_chr17_27353936 CTCCTCCCAGCCCCTCTCGGCGAGCGGGCGCAGGCGCGGTGCGGCCCTTCTCGGCCGGCAGGGGGCGCCGGAGCCCGCCG
GGGCCTCTAGGGCCTCCCCAGCCACGGCCCGGGCCGGCGAAGAGGGAGGGGCAGAGA
mm4__chr9 114.21 Mbp_chr9_114212058 TGGGCATGTTAGCTGTTGGAGCGTTTAACCTGGGCCCTCTGGGGGCCAAAGAGGAAAAACAAAGAAAAGAAAAAAGCCTC
AAAGAGCTGACAGAGGAGGTCCCTCCACCCCCAACCTAGACCCCTCTGT
mm3__chr12 122.05 Mbp_chr12_122054485 CAGCCATGATTGTTGGTGCGCACTGCGGCCGCCAGGGGGTGCTGGGTGCACGCAGCACAGAGACTGCAGCCTCCTCCAGT
CGCCTCCCAGGGCCCTCAGCAGCTAAGTGTGCAGGAG
mm4__chr8 22.55 Mbp_chr8_22551973 TTCTCCTTGCCCTTCCTCCTCGAGCGCCCGCGCCAGGCAGCAGCCGGGCAGGGATGCTCCTGCGCTCCCGGGCGGCCTCG
GGCCCAGCCACCTGCTCGCCGGGGAAGGTAGGTCCGGGCAAG
mm4__chr9 16.42 Mbp_chr9_16419241 CCACCATTGCTCCCAGACAAGCTGCTGAACATAAGAGATCCTGAAACTTCAGCCCCTAGGCTGCCAGGGAGGGCAGGGGC
CTCGGCCTTGTGTGCCGACTCCCCACTGTCACTCGCCCCGTCAATGTCATCGAGA
mm4__chr4 107.93 Mbp_chr4_107932225 GATGAGTCTCCAGGTGCGTGGGGGCTGCAGTTCCTGTGCCCCTTCCGGCCGCCCGCGCCCCCAGGCTGCCTCACACCTGC
ATCCTGAACTAACAGGTGATGGTGGTGGC
mm4__chr7 55.74 Mbp_chr7_55744910 AGGAACTTTTGCACCCGGAGCGGCCGCCGAGAGGCCGGAGCTGGGCCTGGGGAGGCCGACTTGAGGACGACTTGGGCCTG
CAGAGGGCGCCGGGAGGCTGGAGCTGGCCCTGGACAGGCCGACTTGACGACAGTCTGGGC
mm4__chr7 39.79 Mbp_chr7_39792101 ACTGTCATCAAGTCGGCCTGTCCAGGGCCAGCTCCTGCCTCCCGGCGCCCTCTGCAGGCCCAAGTCGTCCTCAAGTTGGC
CTCCCCAGGCCCAGCTCCAGCCTCTCGGCGGCCTCTCCGGGTGCAAAAGTTCC
mm3__chr22 50.29 Mbp_chr22_50291161 ATAGCCCAGCAAGTGCACATGCTGTTTACAACTGCCCGCCAGAGCCCCCAGGCGGCCTGAGCCTCCCTGACCACCTCCAG
GCCCCCTGCCCTGGTGAAACAGTGGAAGCCAGCA
mm3__chr12 120.58 Mbp_chr12_120583560 CACTCCCCACCCAAGAACAACTTTTCACCAGAAAGTGTCTGCGACATCCTCCTTTGGCCAACAGGGGGCGCCAGAGGTCC
GCAGTGCGCGGAGAGAGCAAGACACCC
mm3__chr9 137.02 Mbp_chr9_137023711 ACCAGCTGTGCCTTTAGGTGGCCGGGCCTTTCACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGC
CAGCTGTTCCTGAGGCCGGCAGGGGGCCCGGGAGGGCTGTTAATGCAAGCGGGGAGG
mm4__chr11 68.02 Mbp_chr11_68018651 CCCAAGAACCTGGTGAGCCGGCCGGGCTGAGGCGGGCAGGGGGCTCAGGGGATATTTGCTCCCACAGGGTGGCCCCTCTG
GGTGGGAGCTGGCTGCTGAGGGGCCGGGGTGCTGAGCACTTAATTTCATCCCCGGCTCCC
mm4__chr18 77.04 Mbp_chr18_77043696 AAGCTTTGAACTTGCGTGCGTGCCCCGCACCCCGTGGCGGACTGAGTCACTGCTTTGCTGTTGCGGCGTGGTGAGTAAAA
CTTTCCCAGGGGAGCCGGAGTTGAGTGAGAAAGGGATGCAGGACACG
mm4__chr8 60.91 Mbp_chr8_60910296 GTCCCTCCAGGCTGAGCGCCCAGCGCCCCCCGCGGCCCTAAGTTGGGCCACCTGGCGGGGCGCTGCGGCCGGGCCGGGCG
GGGACATCTGAGGCCCGACAGACCCGCGCTCATGGACAAGTGTCGCCC
mm4__chr1 202.57 Mbp_chr1_202571917 ATCAAAGCCCAATGCCAGGTTTGCAGATCGTCCAGCACCCCCTAGAGGACTAAGGGAGAAGCATCTGTGCCTCTGCATTC
TCACCAGT
mm3__chr22 41.70 Mbp_chr22_41697267 AGAGGAACAGACCGCACATGTCAGAGCCCATTAAGCATTTCCATCCAGCCTGAAAAGGCCAGGAGAGTCAGGCCACCAGG
GGGCGCCAGAGAACCACAGGAGATCCAGGAAGCGCTTGCTAGGACCAAGCTCCC
mm4__chr1 65.25 Mbp_chr1_65254626 GGAAGCTGTTTTCCTTGCGGCGGCCGTGGAAGGCGACCCGGCGGCTGTGGAGGCCACGCTCAGCTCGCCAGGCGGCGCAG
GGTGAGTGTGGGCGCGGCCGGTCGGGACCTGTTACCCTGAGGCAG
mm3__chr19 19.18 Mbp_chr19_19175211 GCAGACAATGACCGCTAGGAGGCTGAACTTCCCGCCAACGCTTTGCCTCTTGCGCCCCCTGGCGGCTAGAGTGGGCGGGG
CGCCCTCCAGGCAAAAAGGGCTATCGAGCATTCAAATGAGATGCAAATTAACCAGCGCCC
mm3__chr2 73.98 Mbp_chr2_73984471 CCATTGTCCTGCCTCGGAATGAGCCAGCGGCAGCTGGGACAGGGGGGTCAGTCGGGCCCGGCGCTCAGGCCTCCCGGGGG
CGCCGGGGCTGTCCCGGACAGATCTGGCCCGCAGGGAGTGGCAGGAAGTG
mm3__chr2 71.94 Mbp_chr2_71944373 GGCTCAGACAAGCAGGGATTCTTCCCAGCGCCCCCGGGAGGCCTGAGGTCCTGCCTCCTGCCTGGGTGGGCATCAGGAAA
GCTCAG
mm3__chr3 98.02 Mbp_chr3_98023410 AGAGCTTCCAACTGTGGCTCCGGAGTGGCCAGTTTCAAGCCATTCCTGTGTGACTGGCGCCCCCTGGTGGCCGAAAACCC
AGGGACAGGCAAATGTA
mm4__chr2 144.44 Mbp_chr2_144441306 CCCATGCCTCAATTCCCCTTCCCTGCCGCCTTCTGTCATTTTCAGGTAGTGGAATTTGCTGACCCTGGCCGCTTGGCGGC
CTAAGCCTGGACGTGGCAAAAGTCAGA
mm3__chr1 244.37 Mbp_chr1_244367648 CAGAGAGCAGAAGACAGCCCAGCCTTAGGCCTCCAGGGGCCCCAGGGCCTGCTGTGCTCAGTTCCACCCAAACCCCCGGA
CTCCCGGCCAGGTCCAGACACCACAGAGCACTAGGCAGGAG
mm4__chr18 26.66 Mbp_chr18_26656184 CAAACTGAATCCCTCCCGCCCACCTCTGCGCCCCGAGCGCTGGTTCAGACGGCGGAGGCCAGAGGCGGCCGGGAAACTGT
CCCTTTAAGGGAACCTAGGCCGCCAGGAGGGGAGGGAAGGATGCGGGCTCCTGACTTG
mm3__chr6 107.85 Mbp_chr6_107848398 CAAGAAGAGGCAGCGGATCTCCCAGTCCCTGACCAGCCTGGCCGGGCCCCGGTCGGCCACCTCGCGCCCCCTGGTGGCCA
CAGAGGGAGAAGCCGCCCGCTCATTTCTCCTGCTG
mm4__chr17 80.11 Mbp_chr17_80112207 CTCAGGGAGGAGGAAAAGCGGAGGCCCAGACCACCCGGGGCCCGCTGGCGGCCCGAGTGCTCTCCCCACCCGCTGCCTGC
ACCCCAGCCTGAAGCTGGAGCGCTCCTTCCCACTTCATGCCTG
mm4__chr17 9.00 Mbp_chr17_8998193 CAGCATTTACTGTGCGAGCCACCTGGGGGCCATTTTTGTTGCCCGAGGTGGTGCGCCTGCGCCACCTGGTGGACTACGGG
AAAGTAGCAGATCGGTGAGATGGGTTGAGGCACCTTCA
mm3__chr1 143.72 Mbp_chr1_143717609 CTTGCCCCTAGCCTGCTTTAGAAGCCCCCGGAAACGCCCCGGCCCCGACCCGGACCTGAGCCACCTGGGGGCCTAAGGGA
AGCTGAATGCCCAGTGGGCTCCCGGGATGGCTCTTCCCGTTCTT
mm4__chr11 62.79 Mbp_chr11_62786684 TTTCAAGAGTCGTCCTCCGGGGGCGGTGCAGAACCCAGCTTTGGGTCCGGCCTCCCGCTGCCGCCAGGGGGCGCGGGGCC
GGAGGACCCTTCTCTTTCG
mm4__chr1_KI270714v1_random 30.75 Kbp_chr1_KI270714v1_random_30752 CTTACATTGCCCATGCTGCCCCTGCTGCCGGCAAGCCCGCTTGCTCCGCTGCCTCCTGCCCAGGCCTGCTCCTCCTTGCC
CCGTGCCCCCTCCCTGCCTAAGAAGTGTCACAGGCACCCTG
mm4__chr16 89.17 Mbp_chr16_89168186 ATACCTGATGGGGCTCCTCTGAGCCCACCTGGACTGACCCCCTGTGGCCGCCAGGGGCAGCTGGTGGCCTGCCTGAATGT
GGGGGCAGTGTCACCCCTGAGCCCAGAGAGCCGGAGCACAGCAGGGTCCAGGATG
mm4__chr8 27.90 Mbp_chr8_27895281 TGCAGAGTAGAGCCCTAGGGATAATTCGGACCATCTGCCCATTAAGGAAGTTGAACTTTCATTCTCTTGTAAAGAAAGAA
CTCCCCGTGCCCCCAGCCGGCCTGAGGCACAGGGGCTCTGCTGAGGCTGAGGTGACACTG
mm4__chr11 2.86 Mbp_chr11_2862409 CTTCGGGGTGCCCAGTCCGAGCCTGGGCCGCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNCGAGCTTTAATGCGCGCACAAA
mm4__chr17 45.89 Mbp_chr17_45894499 CTGGGGAGGCTCGCGTTCCCGCTGCTCGCGCCTGCGCCGCCCGCCGGCCTCAGGAACGCGCCCTCTTCGCCGGCGCGCGC
CCTCGCAGTCACCGCCACCCACCAGCTCCGGCACCAACAG
mm4__chr1 18.30 Mbp_chr1_18295154 AGTCGGGTGGATCATCAGGACTTCTGCCCCCAGGGGGTGCAGGTGTGTATCCNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNATAGAGCGTCCCAGAGGCA
mm4__chr7 55.74 Mbp_chr7_55741124 CCAGGCCAACTTAAGGACGACTTAGGCCTGCAGAGGCCGCCGGGAGGCTGGAGCTGGGCCTGGAGAGGCCGACTTCAGCA
CGATTTAGGCCTGCAGAGGTCG
mm4__chr7 39.79 Mbp_chr7_39794024 GGCCCAAATCGTCCTGAAGTCGGCCTCTCCAGGCCCAGCTCCAGCCTCCCGGCGGCCTCTGCAGGCCTAAGTCGTCCTCA
AGTCGGCCTGGAAATGGGCCTGGAAGAGCT
mm4__chr1 169.01 Mbp_chr1_169013744 TCATTGTGTTGCCTCCAAGCCCCTTGCCCACAGGCGGCCTAAGAGGTGAACATCGCTTTAGAGTAAATGTCATTAATAGT
TTGAATCTCATACTACAGCCTGAAGAAATGGTGAGGG
mm4__chr5 73.12 Mbp_chr5_73120216 CTCGCGCCCCTATAGAACTGGGCCTCAGGCCTCGAGGGGGAGCGGGCCTCGGCGGGCGGGGCGCACAGTTCCGCCAGCCG
GCGGGGGCATCTCAACTCGCC
mm4__chr14 68.63 Mbp_chr14_68628296 CTAAACATGCAGCAGCCAGCAGACTAGAGCCAGCTTCTGCCTCGGGCCGCCCCGGGGCGCGGGATGGGAAGGGGTTAACG
CAG
mm4__chr16 785.77 Kbp_chr16_785771 GTCGTCACCTGTGATCACGGCTGCCTGGCGCCCCCTGCCGGCCCCACCTCAGCCCTTCCTCGCAGGCCTGGCCGGGGCAA
CCCAGTGGGCCTGATGCTGCCTGGCACCTCGAGGCCCCAGAGCCAGTGAGCAGAC
mm4__chr16 66.61 Mbp_chr16_66608706 TGCCTCTAGAGCAGGTCTGTGAGGCAGGGAACCCGGAGAGGGGTCTCTGGCCACCAGGTGGCGCCGGTGCTCCCCACCGG
GCCTACAGGAATGAGCTG
mm4__chr22 41.92 Mbp_chr22_41919575 GGCAACTCTGACTCAGGCTCCAGCTGCCCGTGGGAGGGAGGGGGCGCCCGGGCTCCTGAGGTCGCCAGGGAGCGGCGGGA
CTGGGAGGCTCCAAAGCCCTCAGTGTACGT
mm4__chr4 139.30 Mbp_chr4_139295839 CTCAGTCTCTCCGAGTTGGCAACAGAACCAGCGCCACCTGGCGGCCGGAAGTGCGGGACTCGAGGGCTCTGCAGCAGAGC
TCCGTGGGGGCGTCAACGTGAATTCCAGCACGGCAAGGTTCTCTAGCAC
mm3__chr22 22.86 Mbp_chr22_22855863 AGCTATGACTCAGGGGAGGTTCCTGGGCCTCAGGATCCTCCCTCTGAGGCCACCAGGGGGCGGGGGTGGCACATGCCTGG
ACCTGGGAGGTCCCTGCTGGGCTTCACCCTGGGTGGGTCCTAGGAGCTCCT
mm4__chr16 2.19 Mbp_chr16_2190395 AGGAGACTCAGTGAGGGGAGGCTGTGCCAGCCCCTCCAGGCGGCCTGAGGGCAGGGAGAGGGGGCCAGCCATCTCCCCGG
CAGGCACAAAGCTGCTGGGCTCTCAGTCTGCAGACACTCACTCGTCCACTCG
mm4__chr20 59.93 Mbp_chr20_59934161 ACTCCTGGGTGAGGCCTGGCTCCTGCCGGCAGGGGGCGCAGGGCCGCCTGGGCTCCTGGGTGCGGGTGCCGGAGGGCGGG
AGAGACCGCAGGTCCGGAGACGGGGTGCTGAGCTGGGTACCCAACTGTGTATTCCTAGGC
mm4__chr1 201.11 Mbp_chr1_201112460 TGCTTTCCCTTCCCTGTGTCCCCAATGCCCCCGCCTTGGGGACTAGGCTGGCTGAGGCTGTCGGCTGAGCCTGCCCTGCT
GAGCCAGCCAGGGGGCGGGGGCAGGAGTGGGGGCAGGAGGATTACTCTCCTCGCTCCC
mm4__chr7 75.42 Mbp_chr7_75418655 GGCTTGTGCTTCCTCCAGAAGGGAAAGGGCCCCAGGGCTGCCACTGCCCCCTGGCGGCTGAAGCCAGAGATCCGGGGTAG
GTTTGC
mm4__chr7 73.30 Mbp_chr7_73301281 ACCTTGGCACGTGCTAGAAAGAGCCTTGGACCCTTCCAGCTGCGTAAAGCAAACCTACCCCGGATCTCTGGCTTCAGCCG
CCAGGGGGCAGTGGCAGCCCTGGGGCCCTTTCCCTTCTGGAGGAAGCACAAGCC
mm4__chr7 72.95 Mbp_chr7_72946351 GAAGAGCCTTGGACCCTTCCAGTTGCGTAAAGCAAACCTACCCCGGATCTCTGGCTTCAGCCGCCAGGGGGCAGTGGCAG
CCCTGGGGCCCTTTCCCTTCTGGAGGAAGCACAAGCC
mm4__chr7 128.27 Mbp_chr7_128267848 ATCCCTTGCTGGAGGCTAGATGATGAAAAACAGGATTCTAGAGGAATCCTGTGGCCTCCCGTGCCACCATGCTCATTCTT
GGGCTGCCAGGGGTCGGGGGGTGCTGGGAGGAGGAGTGCAAATG
mm4__chr6 73.16 Mbp_chr6_73157416 GAGTCCACTGTGTCAGGCTTAGGATTCACAATTTCTGGGGGGCAGCGGCAGCTCTGCACAGGCCGCCCGGGGGCGGGGGC
GGTGGGAGCTCAGGGCGCCTGTCCCGGCACCACCACAGATTCCCAGA
mm4__chr6 168.40 Mbp_chr6_168397179 GCAGACAGAGCTCCAGAAGGAGTGCGGTCCAGCTCACCCTGGAGGCCTCAGGGCTCAGCCGCCCACGGCCAGAGGTGAGC
ACATCACAACGTCCTGGACGGTGCCCTCCTCCAGGGACCCTTCCAGCAAAT
mm4__chr17 76.14 Mbp_chr17_76137927 CTGGGCCTCGGTATTCACCGTCAGCGGCCCGGCCCGGGGGACAGCGCTGGGCTCCTGCGCGGCCTGGCGGGCAAAGGCTG
GGTGGATGTGGACAGGGGGCAGTCGCCGCTTCTTGAAAG
mm4__chr16 88.94 Mbp_chr16_88936184 TACTCAGGGAGGTCGGGTACACTGGGCGGGGACTTCCTCATGAGTTCCATGTGTGACCTGGGGCCACCAGGGTGCGCAGG
AGCCCTCCTGACTGCCCCGGGCATTTCCTGGGCGTCTCCCAGGTATCTCCCA
mm4__chr16 30.59 Mbp_chr16_30585406 CTTCCAGCCCACTAGCCTCTGGGGACACCGATCACCGCTTCCCACCGACGGACTGGGGGTCCCGGCCGGAGGGCCCGGCC
TCACCCAGCGCGCCCAGGTGGCCGAAGGTCTCCCGCATCACGTCC
mm4__chr12 113.29 Mbp_chr12_113286415 AAGTCAGGCTGCACTCATGAGGTCTTGGGCCGCGAGGGTGAGCGGGTTAACACGCAGAGCGGAGTTGGGAGGAGCCGACA
GCACGTGTGTGCCCTGCGTGTCTGGAGAGGAGAGAGGGTCC
mm4__chr10 120.46 Mbp_chr10_120461540 GTGGCTCTCTGTCTGCAGGGTCCCCCTAGGAGCTGCTTAGGATGCCAGGGTGCACAGGTAATGGTGTTTACCTCCCCTTA
GATAATCCAGCATCCTTCTTTGAGCTCTTAGGATGCAAGAGGCCTTTGC
mm3__chr14 69.73 Mbp_chr14_69726929 CTGCAGCAGAGGCCGGAGGAAGCGCCACGGGCGGCTCAGACCTGAGGCCGCAAGGGCGCGCCGGGCGGGCAGGGCGGAGG
GTCCGATTTCCTCCCTCTGAACGGAAAAAAAGCCACTTTCCCTGGGAAGAA
mm3__chr13 110.21 Mbp_chr13_110205351 GCTCACAATAGTGCCAGCGTTTACCTGCGGGCCCTGGCGGCCTATGAGTCCTGGGTACCCGGGTTCACCAGGAAAACCCT
GAAACCGAAGAGAGAAGCAGTAACCGTCAGAGGC

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.