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Contents:

PCR primers for off-targets of GGAGGGGAAGACGGCCCGGG GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529558_F TCGTCGGCAGCGTCCTCCCCTACCTGGTGCCT 59.9 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529558_R GTCTCGTGGGCTCGGTGGATGATGATATCGCCGCG 60.4 1.00
mm1__chr2 73.90 Mbp_chr2_73901390_F TCGTCGGCAGCGTCAGGCTTCGCAGGAGATGATG 59.8 0.92
mm1__chr2 73.90 Mbp_chr2_73901390_R GTCTCGTGGGCTCGGTATCCAGATGAGCACGCACC 59.8 0.92
mm2__chr22 15.72 Mbp_chr22_15721562_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.83
mm2__chr22 15.72 Mbp_chr22_15721562_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.83
mm2__chr2 131.26 Mbp_chr2_131263631_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.83
mm2__chr2 131.26 Mbp_chr2_131263631_R GTCTCGTGGGCTCGGCTCTTTCTGATGCATGCCCC 58.9 0.83
mm2__chr2 130.66 Mbp_chr2_130656825_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.83
mm2__chr2 130.66 Mbp_chr2_130656825_R GTCTCGTGGGCTCGGCTCTTTCTGATGCATGCCCC 58.9 0.83
mm2__chr2 130.46 Mbp_chr2_130463844_F TCGTCGGCAGCGTCCTCTTTCTGATGCATGCCCC 58.9 0.83
mm2__chr2 130.46 Mbp_chr2_130463844_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.83
mm2__chr2 130.08 Mbp_chr2_130075272_F TCGTCGGCAGCGTCCTCTTTCTGATGCATGCCCC 58.9 0.83
mm2__chr2 130.08 Mbp_chr2_130075272_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.83
mm2__chr14 19.40 Mbp_chr14_19402809_F TCGTCGGCAGCGTCATCATGTTCTGGTGCCTGGG 60.0 0.83
mm2__chr14 19.40 Mbp_chr14_19402809_R GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG 59.8 0.83
mm2__chr14 19.00 Mbp_chr14_18998943_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.83
mm2__chr14 19.00 Mbp_chr14_18998943_R GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG 60.0 0.83
mm1__chr11 1.80 Mbp_chr11_1803996_F TCGTCGGCAGCGTCCGCCACCCACATAGAAGTCC 60.4 0.69
mm1__chr11 1.80 Mbp_chr11_1803996_R GTCTCGTGGGCTCGGAACACCTCTGGCATGTGCAA 60.4 0.69
mm4__chr11 73.27 Mbp_chr11_73271184_F TCGTCGGCAGCGTCCATCTTACAGACGGGGAGGG 59.2 0.60
mm4__chr11 73.27 Mbp_chr11_73271184_R GTCTCGTGGGCTCGGATCCATCCTTGAGCTTGGGC 60.1 0.60
mm1__chr19 9.52 Mbp_chr19_9518225_F TCGTCGGCAGCGTCAGTTAACAATGGCTCCGGCA 59.9 0.53
mm1__chr19 9.52 Mbp_chr19_9518225_R GTCTCGTGGGCTCGGGGAGTCCTTCTGACCGATGC 60.1 0.53
mm4__chr21 22.91 Mbp_chr21_22907515_F Primer3: not found at this Tm N.d. 0.46
mm4__chr21 22.91 Mbp_chr21_22907515_R Primer3: not found at this Tm N.d. 0.46
mm3__chr1 200.67 Mbp_chr1_200669800_F TCGTCGGCAGCGTCCCAGGGGCCCAAGCTTCC 62.7 0.44
mm3__chr1 200.67 Mbp_chr1_200669800_R GTCTCGTGGGCTCGGGTCCTGACGAGCGGGTCA 61.3 0.44
mm4__chr9 135.68 Mbp_chr9_135677009_F TCGTCGGCAGCGTCTGACACAGAAATGGGGTCCG 59.9 0.43
mm4__chr9 135.68 Mbp_chr9_135677009_R GTCTCGTGGGCTCGGGCTGTCGGGTTAGAAGCCTC 60.4 0.43
mm3__chr11 44.63 Mbp_chr11_44628217_F Primer3: not found at this Tm N.d. 0.43
mm3__chr11 44.63 Mbp_chr11_44628217_R Primer3: not found at this Tm N.d. 0.43
mm4__chr1 154.81 Mbp_chr1_154813398_F TCGTCGGCAGCGTCCTAGGGCGAGGACAAGTGTG 60.1 0.42
mm4__chr1 154.81 Mbp_chr1_154813398_R GTCTCGTGGGCTCGGCCCTGCGGGCTTAAGATGAA 60.1 0.42
mm3__chr11 3.10 Mbp_chr11_3095218_F Primer3: not found at this Tm N.d. 0.42
mm3__chr11 3.10 Mbp_chr11_3095218_R Primer3: not found at this Tm N.d. 0.42
mm4__chr5 1.50 Mbp_chr5_1500505_F TCGTCGGCAGCGTCCTCATCGGGTAGGTGTGCTT 59.4 0.40
mm4__chr5 1.50 Mbp_chr5_1500505_R GTCTCGTGGGCTCGGACTGCATGTCAGCCAACGTT 60.8 0.40
mm4__chr9 136.49 Mbp_chr9_136488355_F TCGTCGGCAGCGTCTGGTGGACATGGACTTGGTG 59.8 0.37
mm4__chr9 136.49 Mbp_chr9_136488355_R GTCTCGTGGGCTCGGGCCTGGCATCGAATACTGGA 59.8 0.37
mm2__chr5 57.48 Mbp_chr5_57482605_F TCGTCGGCAGCGTCATCTCCCACGTAGCAGTCCT 60.0 0.36
mm2__chr5 57.48 Mbp_chr5_57482605_R GTCTCGTGGGCTCGGATGGGTCAGGGATGTGCAAG 60.0 0.36
mm4__chr2 10.23 Mbp_chr2_10231797_F TCGTCGGCAGCGTCACTGCTGGACGAACACAGG 59.9 0.36
mm4__chr2 10.23 Mbp_chr2_10231797_R GTCTCGTGGGCTCGGCCTCTGACAGGCGTTTCCAA 60.2 0.36
mm4__chr2 131.63 Mbp_chr2_131626214_F TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG 59.8 0.34
mm4__chr2 131.63 Mbp_chr2_131626214_R GTCTCGTGGGCTCGGACTCCTTCTGATGCATGCCC 60.1 0.34
mm4__chr21 42.33 Mbp_chr21_42332595_F TCGTCGGCAGCGTCACTTGACCTAACTCACCGGC 59.6 0.33
mm4__chr21 42.33 Mbp_chr21_42332595_R GTCTCGTGGGCTCGGGGTGAAGGCAAGGATCTGCT 60.0 0.33
mm3__chr20 63.31 Mbp_chr20_63305078_F TCGTCGGCAGCGTCATAGGGTAGGAGCCTGTGGG 60.1 0.32
mm3__chr20 63.31 Mbp_chr20_63305078_R GTCTCGTGGGCTCGGGTATGAAGACAGGGCTGCCC 60.4 0.32
mm3__chr20 62.65 Mbp_chr20_62653015_F Primer3: not found at this Tm N.d. 0.31
mm3__chr20 62.65 Mbp_chr20_62653015_R Primer3: not found at this Tm N.d. 0.31
mm4__chr19 966.75 Kbp_chr19_966749_F TCGTCGGCAGCGTCGGAGAAGCTGGAGTCTGCAG 60.1 0.31
mm4__chr19 966.75 Kbp_chr19_966749_R GTCTCGTGGGCTCGGGATCCGAATGCTCATGGGCA 60.5 0.31
mm4__chr9 119.37 Mbp_chr9_119369661_F TCGTCGGCAGCGTCGGTTGCTTCCGAGGAGTGTT 60.2 0.31
mm4__chr9 119.37 Mbp_chr9_119369661_R GTCTCGTGGGCTCGGGACCCACGAAACTCCCTACG 60.1 0.31
mm3__chr4 8.51 Mbp_chr4_8510656_F Primer3: not found at this Tm N.d. 0.30
mm3__chr4 8.51 Mbp_chr4_8510656_R Primer3: not found at this Tm N.d. 0.30
mm4__chr1 102.99 Mbp_chr1_102987693_F Primer3: not found at this Tm N.d. 0.30
mm4__chr1 102.99 Mbp_chr1_102987693_R Primer3: not found at this Tm N.d. 0.30
mm1__chr5 77.78 Mbp_chr5_77784962_F TCGTCGGCAGCGTCCTCGTCATTGACAACGGCTC 59.2 0.30
mm1__chr5 77.78 Mbp_chr5_77784962_R GTCTCGTGGGCTCGGAATCCTTCTGACCCATGCCC 59.7 0.30
mm3__chr19 33.41 Mbp_chr19_33406855_F TCGTCGGCAGCGTCCGCCATTATCTAGCCAGGGA 59.0 0.29
mm3__chr19 33.41 Mbp_chr19_33406855_R GTCTCGTGGGCTCGGCTTTTGTGTTGCTCCTGGGC 59.9 0.29
mm3__chr2 186.59 Mbp_chr2_186589970_F Primer3: not found at this Tm N.d. 0.29
mm3__chr2 186.59 Mbp_chr2_186589970_R Primer3: not found at this Tm N.d. 0.29
mm4__chr10 19.11 Mbp_chr10_19113566_F Primer3: not found at this Tm N.d. 0.28
mm4__chr10 19.11 Mbp_chr10_19113566_R Primer3: not found at this Tm N.d. 0.28
mm4__chr14 104.89 Mbp_chr14_104886983_F TCGTCGGCAGCGTCGGACTTCCACTCCCAGGACA 60.8 0.28
mm4__chr14 104.89 Mbp_chr14_104886983_R GTCTCGTGGGCTCGGCACACTCGGCATCCACAGAA 60.3 0.28
mm3__chr12 124.50 Mbp_chr12_124501911_F Primer3: not found at this Tm N.d. 0.28
mm3__chr12 124.50 Mbp_chr12_124501911_R Primer3: not found at this Tm N.d. 0.28
mm4__chr1 214.55 Mbp_chr1_214551791_F Primer3: not found at this Tm N.d. 0.28
mm4__chr1 214.55 Mbp_chr1_214551791_R Primer3: not found at this Tm N.d. 0.28
mm4__chr1 22.01 Mbp_chr1_22009861_F TCGTCGGCAGCGTCCTCGAGTCTCCGCCTTCATC 59.9 0.27
mm4__chr1 22.01 Mbp_chr1_22009861_R GTCTCGTGGGCTCGGTCAGAAGAGCCACCACCCTA 59.8 0.27
mm4__chr1 21.99 Mbp_chr1_21986687_F TCGTCGGCAGCGTCGGTGTTCACTCGAGTCTCCG 60.1 0.27
mm4__chr1 21.99 Mbp_chr1_21986687_R GTCTCGTGGGCTCGGTCAGAAGAGCCACCACCCTA 59.8 0.27
mm4__chr7 50.56 Mbp_chr7_50560538_F Primer3: not found at this Tm N.d. 0.27
mm4__chr7 50.56 Mbp_chr7_50560538_R Primer3: not found at this Tm N.d. 0.27
mm4__chr15 40.41 Mbp_chr15_40412694_F TCGTCGGCAGCGTCTAAGGGAATTGACCGGGCTG 59.7 0.27
mm4__chr15 40.41 Mbp_chr15_40412694_R GTCTCGTGGGCTCGGTCCCCAGTTCTCATGGGGAC 60.9 0.27
mm2__chr16 8.32 Mbp_chr16_8318062_F TCGTCGGCAGCGTCGCCCCAAAGCATGAATGTGG 60.1 0.26
mm2__chr16 8.32 Mbp_chr16_8318062_R GTCTCGTGGGCTCGGTGTTCAATGTGGCTGGCAAA 58.8 0.26
mm3__chr9 131.56 Mbp_chr9_131555000_F TCGTCGGCAGCGTCGCCCAGCTCTACCCAGCC 62.1 0.26
mm3__chr9 131.56 Mbp_chr9_131555000_R GTCTCGTGGGCTCGGAGACAGCACGGAGGGGAG 60.6 0.26
mm4__chr5 37.25 Mbp_chr5_37249104_F TCGTCGGCAGCGTCTGGGTAGTGGGGATGTACCA 59.5 0.24
mm4__chr5 37.25 Mbp_chr5_37249104_R GTCTCGTGGGCTCGGTACTCTTCTGTCGGGCAGGT 60.2 0.24
mm4__chr7 1.53 Mbp_chr7_1529575_F TCGTCGGCAGCGTCAGTGCAGAACCCGCAAGTG 60.8 0.24
mm4__chr7 1.53 Mbp_chr7_1529575_R GTCTCGTGGGCTCGGATGTGGCTGGGGATTTAGGC 60.1 0.24
mm4__chr15 41.86 Mbp_chr15_41856989_F TCGTCGGCAGCGTCGGCGTCCCTGAAGAACTTCA 59.9 0.24
mm4__chr15 41.86 Mbp_chr15_41856989_R GTCTCGTGGGCTCGGGGTTCAAGAGCCTGAGGGAG 59.7 0.24
mm3__chr6 36.79 Mbp_chr6_36793238_F TCGTCGGCAGCGTCCTCAAACAGAGGAGGCAGGG 60.0 0.24
mm3__chr6 36.79 Mbp_chr6_36793238_R GTCTCGTGGGCTCGGCCGCTCTGCTCACCTGATTT 60.3 0.24
mm4__chr14 73.74 Mbp_chr14_73741441_F TCGTCGGCAGCGTCCATAGCCCACCTCTTTCCCC 59.8 0.23
mm4__chr14 73.74 Mbp_chr14_73741441_R GTCTCGTGGGCTCGGACCTGTAGCATCTAGGGCCT 59.7 0.23
mm4__chr12 111.41 Mbp_chr12_111413614_F TCGTCGGCAGCGTCGCAGCTGTTTTGAGGAAGGC 60.0 0.23
mm4__chr12 111.41 Mbp_chr12_111413614_R GTCTCGTGGGCTCGGACCAGCATGAACCAACCTCC 60.2 0.23
mm3__chr1 115.69 Mbp_chr1_115685390_F Primer3: not found at this Tm N.d. 0.23
mm3__chr1 115.69 Mbp_chr1_115685390_R Primer3: not found at this Tm N.d. 0.23
mm3__chr6 158.81 Mbp_chr6_158806153_F Primer3: not found at this Tm N.d. 0.22
mm3__chr6 158.81 Mbp_chr6_158806153_R Primer3: not found at this Tm N.d. 0.22
mm3__chr9 126.03 Mbp_chr9_126028189_F TCGTCGGCAGCGTCACAGAGCTCTCATCTCCCGA 59.7 0.22
mm3__chr9 126.03 Mbp_chr9_126028189_R GTCTCGTGGGCTCGGGCTGAGCTCAGTGTAGGGTG 60.1 0.22
mm3__chr9 136.70 Mbp_chr9_136696949_F Primer3: not found at this Tm N.d. 0.21
mm3__chr9 136.70 Mbp_chr9_136696949_R Primer3: not found at this Tm N.d. 0.21
mm4__chr20 3.20 Mbp_chr20_3195325_F Primer3: not found at this Tm N.d. 0.21
mm4__chr20 3.20 Mbp_chr20_3195325_R Primer3: not found at this Tm N.d. 0.21
mm4__chr9 114.39 Mbp_chr9_114387826_F TCGTCGGCAGCGTCGTCCCGGAGAGTCTGTGACT 60.6 0.21
mm4__chr9 114.39 Mbp_chr9_114387826_R GTCTCGTGGGCTCGGAGAATTCACAGGCAGGTCCC 59.6 0.21
mm4__chr3 197.51 Mbp_chr3_197510015_F TCGTCGGCAGCGTCTCTGGGCTCTGGAGGGTTT 60.1 0.20
mm4__chr3 197.51 Mbp_chr3_197510015_R GTCTCGTGGGCTCGGAAGAAGGGCCTCACAATGGG 59.9 0.20
mm3__chr16 89.82 Mbp_chr16_89816164_F Primer3: not found at this Tm N.d. 0.20
mm3__chr16 89.82 Mbp_chr16_89816164_R Primer3: not found at this Tm N.d. 0.20
mm4__chr1 32.01 Mbp_chr1_32014784_F TCGTCGGCAGCGTCGATGAGCGCCTCGACCGAG 62.8 0.20
mm4__chr1 32.01 Mbp_chr1_32014784_R GTCTCGTGGGCTCGGCACTGGACAGGGCATTCCAG 60.6 0.20
mm4__chr22 30.91 Mbp_chr22_30905726_F TCGTCGGCAGCGTCAAGGCCAGAGGGAGTCCTG 60.6 0.20
mm4__chr22 30.91 Mbp_chr22_30905726_R GTCTCGTGGGCTCGGTGAGCCGGACCACACCTA 59.8 0.20
mm4__chr19 49.36 Mbp_chr19_49357519_F TCGTCGGCAGCGTCAAGGAGCTCGTGAACACCAC 60.2 0.20
mm4__chr19 49.36 Mbp_chr19_49357519_R GTCTCGTGGGCTCGGTCTCACTGTGCCTGTTTGGG 60.1 0.20
mm4__chr22 22.77 Mbp_chr22_22772713_F TCGTCGGCAGCGTCGGAGGCCTATGGATAGAGCC 58.8 0.20
mm4__chr22 22.77 Mbp_chr22_22772713_R GTCTCGTGGGCTCGGTTCCAGGCCATGTGTATGCC 60.3 0.20
mm4__chr1 31.67 Mbp_chr1_31665449_F TCGTCGGCAGCGTCTTGGGAGCATTACGTCTGCC 60.3 0.20
mm4__chr1 31.67 Mbp_chr1_31665449_R GTCTCGTGGGCTCGGATGGTCCTGGGGAGAAGAGG 60.3 0.20
mm4__chr8 120.83 Mbp_chr8_120834115_F Primer3: not found at this Tm N.d. 0.20
mm4__chr8 120.83 Mbp_chr8_120834115_R Primer3: not found at this Tm N.d. 0.20
mm4__chr16 26.69 Mbp_chr16_26691793_F Primer3: not found at this Tm N.d. 0.20
mm4__chr16 26.69 Mbp_chr16_26691793_R Primer3: not found at this Tm N.d. 0.20
mm4__chr9 127.49 Mbp_chr9_127492142_F Primer3: not found at this Tm N.d. 0.19
mm4__chr9 127.49 Mbp_chr9_127492142_R Primer3: not found at this Tm N.d. 0.19
mm4__chr4 6.52 Mbp_chr4_6521612_F TCGTCGGCAGCGTCGAGTCGTCTCAGGAGCCCTA 60.1 0.19
mm4__chr4 6.52 Mbp_chr4_6521612_R GTCTCGTGGGCTCGGACCAGCTCCTGTGTAAGTGC 59.9 0.19
mm4__chr19 17.51 Mbp_chr19_17510732_F Primer3: not found at this Tm N.d. 0.19
mm4__chr19 17.51 Mbp_chr19_17510732_R Primer3: not found at this Tm N.d. 0.19
mm4__chr9 100.73 Mbp_chr9_100731316_F TCGTCGGCAGCGTCGTCATTGACAACGGCTCAGG 59.2 0.19
mm4__chr9 100.73 Mbp_chr9_100731316_R GTCTCGTGGGCTCGGAAGTCCTTCTGACCCATGCC 59.6 0.19
mm4__chr5 141.75 Mbp_chr5_141748837_F TCGTCGGCAGCGTCATCCCTGAGGTTTGCCCATC 59.7 0.19
mm4__chr5 141.75 Mbp_chr5_141748837_R GTCTCGTGGGCTCGGTCTGGCTCTCACTTCCCACA 60.4 0.19
mm4__chr19 929.79 Kbp_chr19_929794_F Primer3: not found at this Tm N.d. 0.19
mm4__chr19 929.79 Kbp_chr19_929794_R Primer3: not found at this Tm N.d. 0.19
mm4__chr11 116.82 Mbp_chr11_116823353_F Primer3: not found at this Tm N.d. 0.18
mm4__chr11 116.82 Mbp_chr11_116823353_R Primer3: not found at this Tm N.d. 0.18
mm4__chr11 111.22 Mbp_chr11_111221710_F TCGTCGGCAGCGTCGCCAGGGGCTCTTAAGTATGT 59.5 0.18
mm4__chr11 111.22 Mbp_chr11_111221710_R GTCTCGTGGGCTCGGTCTCTCTCTGCTTTCATGGGTG 59.7 0.18
mm4__chr9 35.40 Mbp_chr9_35403655_F TCGTCGGCAGCGTCGGGGTAAGACTTGAGGGGTG 59.3 0.18
mm4__chr9 35.40 Mbp_chr9_35403655_R GTCTCGTGGGCTCGGCCTCATTTCCCAGGAGGCTG 60.1 0.18
mm4__chr5 169.40 Mbp_chr5_169404318_F TCGTCGGCAGCGTCGGGAGGAGACTGGGAGCTTA 60.0 0.18
mm4__chr5 169.40 Mbp_chr5_169404318_R GTCTCGTGGGCTCGGTGGCTGCCTGTGAAGAAACA 60.1 0.18
mm4__chr17 61.41 Mbp_chr17_61408538_F TCGTCGGCAGCGTCGGAGTCGCCCAAGTGAGG 59.7 0.17
mm4__chr17 61.41 Mbp_chr17_61408538_R GTCTCGTGGGCTCGGTGCAGCCCACTTTTCTCCTT 59.8 0.17
mm4__chr11 103.55 Mbp_chr11_103552304_F TCGTCGGCAGCGTCGTCAGCTTGGGAGTGTGTGT 60.1 0.17
mm4__chr11 103.55 Mbp_chr11_103552304_R GTCTCGTGGGCTCGGTCTGTCAGCATTCCCAGCTT 59.3 0.17
mm4__chr21 43.27 Mbp_chr21_43274379_F TCGTCGGCAGCGTCAGTAGATGCTGGAAAGCCGG 59.8 0.17
mm4__chr21 43.27 Mbp_chr21_43274379_R GTCTCGTGGGCTCGGAGGAAACCCACTCCCCTAGT 59.5 0.17
mm4__chr15 77.57 Mbp_chr15_77573223_F Primer3: not found at this Tm N.d. 0.17
mm4__chr15 77.57 Mbp_chr15_77573223_R Primer3: not found at this Tm N.d. 0.17
mm4__chr15 25.90 Mbp_chr15_25904820_F TCGTCGGCAGCGTCGAGCTGTCAGTCTGGGTTCC 60.0 0.17
mm4__chr15 25.90 Mbp_chr15_25904820_R GTCTCGTGGGCTCGGTTGCAGGGGATGAAAAGCCA 60.1 0.17
mm4__chr9 135.67 Mbp_chr9_135666594_F TCGTCGGCAGCGTCCATGGTCCCAGCCCAGTAAA 59.6 0.17
mm4__chr9 135.67 Mbp_chr9_135666594_R GTCTCGTGGGCTCGGTCGTGCAGAAATGCAGAGGT 59.9 0.17
mm2__chr3 175.98 Mbp_chr3_175978480_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 0.17
mm2__chr3 175.98 Mbp_chr3_175978480_R GTCTCGTGGGCTCGGGCAAAGGCTCCTGTATGTGC 59.5 0.17
mm4__chr20 32.43 Mbp_chr20_32434635_F TCGTCGGCAGCGTCCACCACTGCCATAGAGAGGC 60.1 0.17
mm4__chr20 32.43 Mbp_chr20_32434635_R GTCTCGTGGGCTCGGCCTCACCACCATCACCACTG 60.3 0.17
mm4__chr16 51.55 Mbp_chr16_51552057_F Primer3: not found at this Tm N.d. 0.17
mm4__chr16 51.55 Mbp_chr16_51552057_R Primer3: not found at this Tm N.d. 0.17
mm4__chr2 7.08 Mbp_chr2_7076163_F Primer3: not found at this Tm N.d. 0.16
mm4__chr2 7.08 Mbp_chr2_7076163_R Primer3: not found at this Tm N.d. 0.16
mm4__chr3 126.93 Mbp_chr3_126930473_F Primer3: not found at this Tm N.d. 0.16
mm4__chr3 126.93 Mbp_chr3_126930473_R Primer3: not found at this Tm N.d. 0.16
mm4__chr15 75.58 Mbp_chr15_75579522_F Primer3: not found at this Tm N.d. 0.15
mm4__chr15 75.58 Mbp_chr15_75579522_R Primer3: not found at this Tm N.d. 0.15
mm4__chr1 147.59 Mbp_chr1_147591706_F TCGTCGGCAGCGTCTTCAGTCTCTGGCTTGGCTC 59.6 0.15
mm4__chr1 147.59 Mbp_chr1_147591706_R GTCTCGTGGGCTCGGAGGATCAGAAGCCAAACTGG 57.4 0.15
mm4__chr6 34.06 Mbp_chr6_34056709_F TCGTCGGCAGCGTCGAGAAGTAGCGGTCGAAGCC 60.5 0.15
mm4__chr6 34.06 Mbp_chr6_34056709_R GTCTCGTGGGCTCGGTCCGCCTGGACCATCTCTTA 60.0 0.15
mm4__chr19 48.33 Mbp_chr19_48333573_F TCGTCGGCAGCGTCGGGTTTGGGAGAAGCCTAGG 59.7 0.15
mm4__chr19 48.33 Mbp_chr19_48333573_R GTCTCGTGGGCTCGGCAAGAGCCCCTGACTGGTC 59.7 0.15
mm2__chr14 104.32 Mbp_chr14_104319518_F Primer3: not found at this Tm N.d. 0.15
mm2__chr14 104.32 Mbp_chr14_104319518_R Primer3: not found at this Tm N.d. 0.15
mm4__chr3 127.03 Mbp_chr3_127028940_F TCGTCGGCAGCGTCCCGTCCCACTACACTGCTG 60.0 0.15
mm4__chr3 127.03 Mbp_chr3_127028940_R GTCTCGTGGGCTCGGATCTTGGTGGTGACGTCCTC 59.3 0.15
mm4__chr5 133.78 Mbp_chr5_133779936_F Primer3: not found at this Tm N.d. 0.15
mm4__chr5 133.78 Mbp_chr5_133779936_R Primer3: not found at this Tm N.d. 0.15
mm4__chr1 117.84 Mbp_chr1_117843029_F Primer3: not found at this Tm N.d. 0.15
mm4__chr1 117.84 Mbp_chr1_117843029_R Primer3: not found at this Tm N.d. 0.15
mm4__chr7 1.73 Mbp_chr7_1725516_F Primer3: not found at this Tm N.d. 0.14
mm4__chr7 1.73 Mbp_chr7_1725516_R Primer3: not found at this Tm N.d. 0.14
mm4__chr11 477.98 Kbp_chr11_477980_F TCGTCGGCAGCGTCGGGCGTTTGTATTTCCTGCC 59.8 0.14
mm4__chr11 477.98 Kbp_chr11_477980_R GTCTCGTGGGCTCGGGTGCAGACGTGAGGTTTTCG 59.7 0.14
mm4__chr7 100.01 Mbp_chr7_100009777_F Primer3: not found at this Tm N.d. 0.14
mm4__chr7 100.01 Mbp_chr7_100009777_R Primer3: not found at this Tm N.d. 0.14
mm4__chr7 100.01 Mbp_chr7_100009716_F TCGTCGGCAGCGTCGCGGGTGATTCAACAAAGCC 60.3 0.14
mm4__chr7 100.01 Mbp_chr7_100009716_R GTCTCGTGGGCTCGGTGAATGGAACGTGGACCTCC 59.6 0.14
mm4__chr10 129.95 Mbp_chr10_129945179_F Primer3: not found at this Tm N.d. 0.14
mm4__chr10 129.95 Mbp_chr10_129945179_R Primer3: not found at this Tm N.d. 0.14
mm4__chr10 130.22 Mbp_chr10_130215604_F Primer3: not found at this Tm N.d. 0.14
mm4__chr10 130.22 Mbp_chr10_130215604_R Primer3: not found at this Tm N.d. 0.14
mm4__chr4 145.18 Mbp_chr4_145179759_F Primer3: not found at this Tm N.d. 0.14
mm4__chr4 145.18 Mbp_chr4_145179759_R Primer3: not found at this Tm N.d. 0.14
mm4__chr19 39.19 Mbp_chr19_39187774_F TCGTCGGCAGCGTCCTAGGCTTGGGCAAGTTGGA 59.9 0.14
mm4__chr19 39.19 Mbp_chr19_39187774_R GTCTCGTGGGCTCGGGACCTGACGTTGCTCTGCTT 60.6 0.14
mm4__chr6 36.32 Mbp_chr6_36320276_F Primer3: not found at this Tm N.d. 0.13
mm4__chr6 36.32 Mbp_chr6_36320276_R Primer3: not found at this Tm N.d. 0.13
mm4__chr16 173.64 Kbp_chr16_173640_F TCGTCGGCAGCGTCCACCGTGCTGACCTCCAAAT 60.6 0.13
mm4__chr16 173.64 Kbp_chr16_173640_R GTCTCGTGGGCTCGGCTGCTGCCCACTCAGACTTT 60.2 0.13
mm4__chr1 7.05 Mbp_chr1_7050736_F TCGTCGGCAGCGTCGGTGGGTGGAGGAGAGAGTA 59.6 0.13
mm4__chr1 7.05 Mbp_chr1_7050736_R GTCTCGTGGGCTCGGAGAAACTCGTGGAGGCCAAG 59.9 0.13
mm4__chr21 6.08 Mbp_chr21_6075912_F TCGTCGGCAGCGTCGAGTGTGGGGTGTGTGAGAC 60.2 0.13
mm4__chr21 6.08 Mbp_chr21_6075912_R GTCTCGTGGGCTCGGGCCATCTGCAGAGTGGGAAC 61.0 0.13
mm4__chr21 43.46 Mbp_chr21_43462329_F Primer3: not found at this Tm N.d. 0.13
mm4__chr21 43.46 Mbp_chr21_43462329_R Primer3: not found at this Tm N.d. 0.13
mm4__chrX 73.08 Mbp_chrX_73079604_F Primer3: not found at this Tm N.d. 0.13
mm4__chrX 73.08 Mbp_chrX_73079604_R Primer3: not found at this Tm N.d. 0.13
mm4__chrX 73.00 Mbp_chrX_73003395_F Primer3: not found at this Tm N.d. 0.13
mm4__chrX 73.00 Mbp_chrX_73003395_R Primer3: not found at this Tm N.d. 0.13
mm4__chr2 109.47 Mbp_chr2_109472309_F Primer3: not found at this Tm N.d. 0.13
mm4__chr2 109.47 Mbp_chr2_109472309_R Primer3: not found at this Tm N.d. 0.13
mm4__chr7 101.55 Mbp_chr7_101550972_F TCGTCGGCAGCGTCGGACTGGAGGCCTTTGAGTC 60.0 0.13
mm4__chr7 101.55 Mbp_chr7_101550972_R GTCTCGTGGGCTCGGCACTGTGCTCTGAGTCTGCA 59.9 0.13
mm4__chr8 27.52 Mbp_chr8_27524659_F Primer3: not found at this Tm N.d. 0.13
mm4__chr8 27.52 Mbp_chr8_27524659_R Primer3: not found at this Tm N.d. 0.13
mm4__chr15 82.34 Mbp_chr15_82336150_F TCGTCGGCAGCGTCGAGCATCAGAGGCTGAACCC 60.4 0.13
mm4__chr15 82.34 Mbp_chr15_82336150_R GTCTCGTGGGCTCGGTTTGAGGAGCAGCAGGACAG 59.9 0.13
mm4__chr20 38.60 Mbp_chr20_38599569_F TCGTCGGCAGCGTCTGTGCTGTAGTGTGGAGTGG 59.6 0.13
mm4__chr20 38.60 Mbp_chr20_38599569_R GTCTCGTGGGCTCGGGTCCCTCAGACCCAGAAAGC 60.0 0.13
mm4__chr17 66.75 Mbp_chr17_66753747_F Primer3: not found at this Tm N.d. 0.13
mm4__chr17 66.75 Mbp_chr17_66753747_R Primer3: not found at this Tm N.d. 0.13
mm4__chr6 34.22 Mbp_chr6_34224039_F TCGTCGGCAGCGTCCATTCCCGAGCTCCGCCC 62.9 0.12
mm4__chr6 34.22 Mbp_chr6_34224039_R GTCTCGTGGGCTCGGCTCATGCGTGTGACTCAGG 58.2 0.12
mm4__chr5 38.54 Mbp_chr5_38544389_F Primer3: not found at this Tm N.d. 0.12
mm4__chr5 38.54 Mbp_chr5_38544389_R Primer3: not found at this Tm N.d. 0.12
mm2__chr13 111.92 Mbp_chr13_111915153_F TCGTCGGCAGCGTCGGTGACTCAAGAGACCGTGG 60.0 0.12
mm2__chr13 111.92 Mbp_chr13_111915153_R GTCTCGTGGGCTCGGCTGAGATCTGCAGGCAGGTG 60.4 0.12
mm4__chr17 4.51 Mbp_chr17_4513388_F TCGTCGGCAGCGTCCCTGTCCTGTTGGTCGTCAT 59.6 0.12
mm4__chr17 4.51 Mbp_chr17_4513388_R GTCTCGTGGGCTCGGGGATGCAAAGCCCACTCTCT 60.0 0.12
mm4__chr12 132.32 Mbp_chr12_132321979_F Primer3: not found at this Tm N.d. 0.12
mm4__chr12 132.32 Mbp_chr12_132321979_R Primer3: not found at this Tm N.d. 0.12
mm4__chr14 37.20 Mbp_chr14_37197809_F TCGTCGGCAGCGTCGCTCGTCGTCTTGGATTCGA 60.1 0.12
mm4__chr14 37.20 Mbp_chr14_37197809_R GTCTCGTGGGCTCGGGGGCCTCCAGGAGAAAGAGA 60.6 0.12
mm4__chr6 46.21 Mbp_chr6_46205051_F TCGTCGGCAGCGTCCGGCATGTGAAAAGCAGCTT 60.0 0.12
mm4__chr6 46.21 Mbp_chr6_46205051_R GTCTCGTGGGCTCGGCACGTAGGAGTCCTTCTGGC 59.8 0.12
mm4__chr20 57.53 Mbp_chr20_57526424_F TCGTCGGCAGCGTCGGAATGAGGAGGGGAGGAGA 59.7 0.12
mm4__chr20 57.53 Mbp_chr20_57526424_R GTCTCGTGGGCTCGGGGCCATCCTCCCCTCCTTAT 60.4 0.12
mm4__chr18 79.61 Mbp_chr18_79609960_F TCGTCGGCAGCGTCGGAAGATGGGACCCCTTGTG 60.0 0.12
mm4__chr18 79.61 Mbp_chr18_79609960_R GTCTCGTGGGCTCGGCTTTCCACTCACCCAGGCAA 60.1 0.12
mm4__chr2 231.40 Mbp_chr2_231399836_F Primer3: not found at this Tm N.d. 0.12
mm4__chr2 231.40 Mbp_chr2_231399836_R Primer3: not found at this Tm N.d. 0.12
mm4__chr12 6.53 Mbp_chr12_6533828_F TCGTCGGCAGCGTCACCTTCCGTGCAGAAACCTC 60.2 0.12
mm4__chr12 6.53 Mbp_chr12_6533828_R GTCTCGTGGGCTCGGCCCCATCTCCTGGCTCCT 60.0 0.12
mm3__chr16 80.61 Mbp_chr16_80612126_F Primer3: not found at this Tm N.d. 0.12
mm3__chr16 80.61 Mbp_chr16_80612126_R Primer3: not found at this Tm N.d. 0.12
mm4__chr1 52.55 Mbp_chr1_52552696_F TCGTCGGCAGCGTCTACAACAGGTTTCGGTCCGG 59.9 0.12
mm4__chr1 52.55 Mbp_chr1_52552696_R GTCTCGTGGGCTCGGCCCAGGGGAAGGCTGAGATA 60.4 0.12
mm4__chr17 42.17 Mbp_chr17_42168716_F Primer3: not found at this Tm N.d. 0.12
mm4__chr17 42.17 Mbp_chr17_42168716_R Primer3: not found at this Tm N.d. 0.12
mm4__chr11 76.19 Mbp_chr11_76190053_F TCGTCGGCAGCGTCTCATGAGGAGTGAGGGGCAG 60.9 0.12
mm4__chr11 76.19 Mbp_chr11_76190053_R GTCTCGTGGGCTCGGTCCAAGCGCACTTACCTAGC 60.1 0.12
mm1__chr6 88.28 Mbp_chr6_88276908_F TCGTCGGCAGCGTCGAGTCCTTCTGACCCATGCC 60.1 0.12
mm1__chr6 88.28 Mbp_chr6_88276908_R GTCTCGTGGGCTCGGTGGATGATGCTATCACCGCG 60.3 0.12
mm4__chr17 42.80 Mbp_chr17_42796801_F TCGTCGGCAGCGTCACTGAGCAAGGAAGATCCCA 58.3 0.12
mm4__chr17 42.80 Mbp_chr17_42796801_R GTCTCGTGGGCTCGGACACCTCCACACAGCAAACA 60.0 0.12
mm4__chr12 132.14 Mbp_chr12_132144080_F Primer3: not found at this Tm N.d. 0.12
mm4__chr12 132.14 Mbp_chr12_132144080_R Primer3: not found at this Tm N.d. 0.12
mm4__chr12 132.51 Mbp_chr12_132510784_F Primer3: not found at this Tm N.d. 0.11
mm4__chr12 132.51 Mbp_chr12_132510784_R Primer3: not found at this Tm N.d. 0.11
mm4__chr8 12.95 Mbp_chr8_12951417_F TCGTCGGCAGCGTCGTCGTCGTCACCCAAGGTAC 60.3 0.11
mm4__chr8 12.95 Mbp_chr8_12951417_R GTCTCGTGGGCTCGGCCTTGTTGCCTGCCTCTCTC 60.6 0.11
mm4__chr5 172.18 Mbp_chr5_172179878_F Primer3: not found at this Tm N.d. 0.11
mm4__chr5 172.18 Mbp_chr5_172179878_R Primer3: not found at this Tm N.d. 0.11
mm4__chr16 73.07 Mbp_chr16_73066590_F TCGTCGGCAGCGTCCTTAGGCCAGTGCAGAGTCG 60.4 0.11
mm4__chr16 73.07 Mbp_chr16_73066590_R GTCTCGTGGGCTCGGGGGCATAATGGGGTGATCGA 59.6 0.11
mm4__chr11 47.77 Mbp_chr11_47767266_F TCGTCGGCAGCGTCCGGAGAGAGTTGCAGGATGG 60.1 0.11
mm4__chr11 47.77 Mbp_chr11_47767266_R GTCTCGTGGGCTCGGCTCTCTGCTCGCGCTTGG 60.8 0.11
mm4__chr19 11.44 Mbp_chr19_11441326_F TCGTCGGCAGCGTCGAGAGTCCCTGCAGCAGATG 60.1 0.11
mm4__chr19 11.44 Mbp_chr19_11441326_R GTCTCGTGGGCTCGGCGGGTTCCTTACCTGCTTCT 59.3 0.11
mm4__chr15 24.96 Mbp_chr15_24956470_F TCGTCGGCAGCGTCTTCGGAGTTTCAGCCGTACC 60.0 0.11
mm4__chr15 24.96 Mbp_chr15_24956470_R GTCTCGTGGGCTCGGTCCTTTCCCAAGCAGCACTT 59.8 0.11
mm4__chr1 2.87 Mbp_chr1_2868344_F TCGTCGGCAGCGTCAGGCACCAGGGGTCAGTG 61.2 0.11
mm4__chr1 2.87 Mbp_chr1_2868344_R GTCTCGTGGGCTCGGGACAGAGACGTATGGCCCAG 59.8 0.11
mm4__chr9 133.69 Mbp_chr9_133689888_F Primer3: not found at this Tm N.d. 0.11
mm4__chr9 133.69 Mbp_chr9_133689888_R Primer3: not found at this Tm N.d. 0.11
mm4__chr7 2.66 Mbp_chr7_2661760_F TCGTCGGCAGCGTCCCTCCAGCATGAGAGCCAAA 60.0 0.11
mm4__chr7 2.66 Mbp_chr7_2661760_R GTCTCGTGGGCTCGGCAGGTGGAAGCGGCAGTG 61.0 0.11
mm4__chr10 28.53 Mbp_chr10_28533187_F Primer3: not found at this Tm N.d. 0.11
mm4__chr10 28.53 Mbp_chr10_28533187_R Primer3: not found at this Tm N.d. 0.11
mm4__chr9 89.42 Mbp_chr9_89422506_F TCGTCGGCAGCGTCTGCTGGCTCTGTAACATGGG 60.0 0.11
mm4__chr9 89.42 Mbp_chr9_89422506_R GTCTCGTGGGCTCGGCACAGGACCTGGACGTGAC 60.0 0.11
mm4__chr5 93.60 Mbp_chr5_93604272_F TCGTCGGCAGCGTCGGGCTTAACAAAGGGCTCCG 61.3 0.10
mm4__chr5 93.60 Mbp_chr5_93604272_R GTCTCGTGGGCTCGGTATGTCAAGAAGCCCCACGG 59.7 0.10
mm4__chr22 29.72 Mbp_chr22_29721106_F TCGTCGGCAGCGTCGTAGCCCCTTCCCTGCAG 59.4 0.10
mm4__chr22 29.72 Mbp_chr22_29721106_R GTCTCGTGGGCTCGGCCCTTCCAGGCTTGGCTATC 60.1 0.10
mm4__chr19 55.28 Mbp_chr19_55284241_F Primer3: not found at this Tm N.d. 0.10
mm4__chr19 55.28 Mbp_chr19_55284241_R Primer3: not found at this Tm N.d. 0.10
mm4__chrX 119.66 Mbp_chrX_119656480_F Primer3: not found at this Tm N.d. 0.10
mm4__chrX 119.66 Mbp_chrX_119656480_R Primer3: not found at this Tm N.d. 0.10
mm4__chr19 41.97 Mbp_chr19_41969210_F TCGTCGGCAGCGTCGGGCTTTGAAGGGACAGACA 59.8 0.10
mm4__chr19 41.97 Mbp_chr19_41969210_R GTCTCGTGGGCTCGGCAGCATCAAGGCCTTTGCTG 60.1 0.10
mm4__chr5 78.51 Mbp_chr5_78510274_F TCGTCGGCAGCGTCTGATGTTCCGGGCGAAGAAA 59.9 0.10
mm4__chr5 78.51 Mbp_chr5_78510274_R GTCTCGTGGGCTCGGGGGAGTGAATGACTGCAGCT 60.0 0.10
mm4__chr1 229.43 Mbp_chr1_229433010_F TCGTCGGCAGCGTCGCAGCCTGACCTGGTGTC 60.3 0.10
mm4__chr1 229.43 Mbp_chr1_229433010_R GTCTCGTGGGCTCGGGACGAAGACGAGACCACCG 60.1 0.10
mm4__chr4 189.51 Mbp_chr4_189513003_F TCGTCGGCAGCGTCAGATGCCTCTAGAGCTCAGC 58.6 0.10
mm4__chr4 189.51 Mbp_chr4_189513003_R GTCTCGTGGGCTCGGCTCCCTCCTTGGTTAGGCAG 59.4 0.10
mm4__chr11 116.51 Mbp_chr11_116513757_F Primer3: not found at this Tm N.d. 0.09
mm4__chr11 116.51 Mbp_chr11_116513757_R Primer3: not found at this Tm N.d. 0.09
mm4__chr17 28.26 Mbp_chr17_28256362_F TCGTCGGCAGCGTCGGCCAGGGAAGAGGAAAAGG 60.3 0.09
mm4__chr17 28.26 Mbp_chr17_28256362_R GTCTCGTGGGCTCGGGCGCTGGAACTGTAGCCTAG 60.5 0.09
mm4__chr16 169.38 Kbp_chr16_169383_F TCGTCGGCAGCGTCTGTGAGTGCTGTGTTGACCT 59.4 0.09
mm4__chr16 169.38 Kbp_chr16_169383_R GTCTCGTGGGCTCGGAAGATCAGGAAGTGCTGGGG 59.3 0.09
mm4__chr10 131.70 Mbp_chr10_131695389_F TCGTCGGCAGCGTCTCCAGGCTGGAAAGATGCTG 60.0 0.09
mm4__chr10 131.70 Mbp_chr10_131695389_R GTCTCGTGGGCTCGGAGCCAAGCAGATCCTGGAAC 60.0 0.09
mm4__chr19 14.57 Mbp_chr19_14565697_F TCGTCGGCAGCGTCTGGATCGGTTTCGCCATCAT 59.8 0.09
mm4__chr19 14.57 Mbp_chr19_14565697_R GTCTCGTGGGCTCGGAGGAAAGAACGGGCGTCAG 60.0 0.09
mm4__chr19 19.06 Mbp_chr19_19056742_F TCGTCGGCAGCGTCCTGAACCCAGACTCCTCCGT 60.9 0.09
mm4__chr19 19.06 Mbp_chr19_19056742_R GTCTCGTGGGCTCGGCCAGAGTAAGAGCCAGCGAG 59.8 0.09
mm4__chr11 64.76 Mbp_chr11_64759194_F TCGTCGGCAGCGTCAACTAGGATGCTGCCAGTGC 60.3 0.09
mm4__chr11 64.76 Mbp_chr11_64759194_R GTCTCGTGGGCTCGGGAGATCAAGTGTCCGGTGGG 60.1 0.09
mm4__chr20 58.65 Mbp_chr20_58651205_F TCGTCGGCAGCGTCGAACGTTGCGAGCAAGGCTT 62.1 0.09
mm4__chr20 58.65 Mbp_chr20_58651205_R GTCTCGTGGGCTCGGGGATCTGACTGCAGCTCAGC 60.8 0.09
mm4__chr10 71.74 Mbp_chr10_71742334_F TCGTCGGCAGCGTCCACCAATGTGCATGGTGGTG 60.0 0.09
mm4__chr10 71.74 Mbp_chr10_71742334_R GTCTCGTGGGCTCGGACCCTGACGTGTTGTGACAG 60.1 0.09
mm4__chr2 19.80 Mbp_chr2_19799445_F TCGTCGGCAGCGTCGCCGAAGAGTCCAAAACAGC 59.7 0.09
mm4__chr2 19.80 Mbp_chr2_19799445_R GTCTCGTGGGCTCGGTCCTGGCCACACCTTATTGC 60.3 0.09
mm4__chr10 69.02 Mbp_chr10_69022829_F Primer3: not found at this Tm N.d. 0.09
mm4__chr10 69.02 Mbp_chr10_69022829_R Primer3: not found at this Tm N.d. 0.09
mm4__chr5 6.08 Mbp_chr5_6083772_F TCGTCGGCAGCGTCTCACGGCTGTGTCAGGAAAA 59.8 0.09
mm4__chr5 6.08 Mbp_chr5_6083772_R GTCTCGTGGGCTCGGGGCAGGGGAGTAGACGGATA 60.1 0.09
mm4__chr7 70.47 Mbp_chr7_70474285_F TCGTCGGCAGCGTCCTCACAGAGGGCAACACGTA 59.6 0.09
mm4__chr7 70.47 Mbp_chr7_70474285_R GTCTCGTGGGCTCGGAGTGCTACAAGGACACAGGC 59.9 0.09
mm4__chr17 77.27 Mbp_chr17_77270437_F Primer3: not found at this Tm N.d. 0.09
mm4__chr17 77.27 Mbp_chr17_77270437_R Primer3: not found at this Tm N.d. 0.09
mm4__chr9 114.10 Mbp_chr9_114102123_F Primer3: not found at this Tm N.d. 0.09
mm4__chr9 114.10 Mbp_chr9_114102123_R Primer3: not found at this Tm N.d. 0.09
mm4__chr1 10.72 Mbp_chr1_10717088_F Primer3: not found at this Tm N.d. 0.09
mm4__chr1 10.72 Mbp_chr1_10717088_R Primer3: not found at this Tm N.d. 0.09
mm4__chr8 102.56 Mbp_chr8_102564519_F TCGTCGGCAGCGTCACAGTGTGGGAGTGAAGTGC 60.1 0.08
mm4__chr8 102.56 Mbp_chr8_102564519_R GTCTCGTGGGCTCGGGGACTAAGCCCTGCTCTGTG 60.1 0.08
mm3__chr9 135.08 Mbp_chr9_135080244_F TCGTCGGCAGCGTCGGTGGTCTGGATGAGCTGTC 60.1 0.08
mm3__chr9 135.08 Mbp_chr9_135080244_R GTCTCGTGGGCTCGGTCCTCCCAGCAAGTCCAAAA 59.1 0.08
mm3__chr2 238.72 Mbp_chr2_238720744_F TCGTCGGCAGCGTCCAGAAGAGGTGCACTGGGAG 60.0 0.08
mm3__chr2 238.72 Mbp_chr2_238720744_R GTCTCGTGGGCTCGGTGTTTGGTCGATACAGACGCA 60.0 0.08
mm4__chr20 51.45 Mbp_chr20_51445246_F Primer3: not found at this Tm N.d. 0.08
mm4__chr20 51.45 Mbp_chr20_51445246_R Primer3: not found at this Tm N.d. 0.08
mm3__chr12 48.18 Mbp_chr12_48184029_F TCGTCGGCAGCGTCCTCCGCTTCCCTCGGCTC 62.2 0.08
mm3__chr12 48.18 Mbp_chr12_48184029_R GTCTCGTGGGCTCGGGCAGACCGTCCTCCATTGAG 60.4 0.08
mm4__chr17 36.88 Mbp_chr17_36884620_F TCGTCGGCAGCGTCCCGCTGCAGAAGGGATATGT 59.8 0.08
mm4__chr17 36.88 Mbp_chr17_36884620_R GTCTCGTGGGCTCGGCTGACAACCCCTCACCCATC 60.0 0.08
mm3__chr3 12.07 Mbp_chr3_12070300_F TCGTCGGCAGCGTCTCATTGACAGTGTCTCCGGC 60.0 0.08
mm3__chr3 12.07 Mbp_chr3_12070300_R GTCTCGTGGGCTCGGGAGTCCTTCTGACCCATGCC 60.1 0.08
mm4__chr19 42.21 Mbp_chr19_42207481_F Primer3: not found at this Tm N.d. 0.08
mm4__chr19 42.21 Mbp_chr19_42207481_R Primer3: not found at this Tm N.d. 0.08
mm4__chr11 67.38 Mbp_chr11_67379800_F TCGTCGGCAGCGTCTCCCTGGGCTGGAAGATCTT 60.2 0.08
mm4__chr11 67.38 Mbp_chr11_67379800_R GTCTCGTGGGCTCGGAAAGCTGGGAGAAGCCTGAC 59.9 0.08
mm4__chr20 58.93 Mbp_chr20_58927755_F Primer3: not found at this Tm N.d. 0.08
mm4__chr20 58.93 Mbp_chr20_58927755_R Primer3: not found at this Tm N.d. 0.08
mm4__chr22 42.40 Mbp_chr22_42397607_F Primer3: not found at this Tm N.d. 0.08
mm4__chr22 42.40 Mbp_chr22_42397607_R Primer3: not found at this Tm N.d. 0.08
mm4__chr8 139.59 Mbp_chr8_139593373_F Primer3: not found at this Tm N.d. 0.08
mm4__chr8 139.59 Mbp_chr8_139593373_R Primer3: not found at this Tm N.d. 0.08
mm4__chr17 4.98 Mbp_chr17_4982788_F TCGTCGGCAGCGTCCCCTGGACCTTCAGCTTCAT 59.3 0.08
mm4__chr17 4.98 Mbp_chr17_4982788_R GTCTCGTGGGCTCGGCTTCCCAGGCCTCAGAATGG 60.1 0.08
mm4__chr7 44.18 Mbp_chr7_44176862_F TCGTCGGCAGCGTCAATCACCCGATGCTTCCCTG 60.1 0.08
mm4__chr7 44.18 Mbp_chr7_44176862_R GTCTCGTGGGCTCGGATCCCCATGAGAACTGCAGC 60.1 0.08
mm4__chr1 29.93 Mbp_chr1_29926912_F TCGTCGGCAGCGTCATTAGATGCCAGCCACGAGG 59.8 0.07
mm4__chr1 29.93 Mbp_chr1_29926912_R GTCTCGTGGGCTCGGCCACCTAGGGAGCAGTGTTG 60.0 0.07
mm4__chr3 32.24 Mbp_chr3_32238856_F Primer3: not found at this Tm N.d. 0.07
mm4__chr3 32.24 Mbp_chr3_32238856_R Primer3: not found at this Tm N.d. 0.07
mm4__chr5 218.90 Kbp_chr5_218897_F Primer3: not found at this Tm N.d. 0.07
mm4__chr5 218.90 Kbp_chr5_218897_R Primer3: not found at this Tm N.d. 0.07
mm4__chr11 72.68 Mbp_chr11_72678319_F TCGTCGGCAGCGTCGGTCCCTAAGCACACTCTGG 59.7 0.07
mm4__chr11 72.68 Mbp_chr11_72678319_R GTCTCGTGGGCTCGGCAGCAATGGTGGGCTAGGAT 59.8 0.07
mm4__chr12 115.18 Mbp_chr12_115179050_F Primer3: not found at this Tm N.d. 0.07
mm4__chr12 115.18 Mbp_chr12_115179050_R Primer3: not found at this Tm N.d. 0.07
mm4__chr2 17.75 Mbp_chr2_17753339_F TCGTCGGCAGCGTCGACCTCTCCCCGCTTTACAG 59.8 0.07
mm4__chr2 17.75 Mbp_chr2_17753339_R GTCTCGTGGGCTCGGAAACCTGGCGTCTGCTCC 59.9 0.07
mm4__chr6 167.98 Mbp_chr6_167977394_F TCGTCGGCAGCGTCCTTCTCTCCCCTCCCACCC 60.6 0.07
mm4__chr6 167.98 Mbp_chr6_167977394_R GTCTCGTGGGCTCGGATGGGAGGGGAGAAGAGGTC 60.0 0.07
mm4__chr22 33.92 Mbp_chr22_33920326_F Primer3: not found at this Tm N.d. 0.07
mm4__chr22 33.92 Mbp_chr22_33920326_R Primer3: not found at this Tm N.d. 0.07
mm4__chr7 154.38 Mbp_chr7_154375320_F Primer3: not found at this Tm N.d. 0.07
mm4__chr7 154.38 Mbp_chr7_154375320_R Primer3: not found at this Tm N.d. 0.07
mm4__chr1 48.78 Mbp_chr1_48776528_F TCGTCGGCAGCGTCGGAAGGAGCAAGCAGTGGC 61.3 0.07
mm4__chr1 48.78 Mbp_chr1_48776528_R GTCTCGTGGGCTCGGCCACAAGGCCCAGATCCTG 60.0 0.07
mm4__chr10 114.04 Mbp_chr10_114043530_F TCGTCGGCAGCGTCCAGGAGTGGGTGCGGGAG 62.0 0.07
mm4__chr10 114.04 Mbp_chr10_114043530_R GTCTCGTGGGCTCGGCTGGTTCCTGGTCCCTGTTC 59.9 0.07
mm4__chr19 36.14 Mbp_chr19_36142599_F TCGTCGGCAGCGTCTTTCCTCATCAAGCTGCCCA 59.5 0.07
mm4__chr19 36.14 Mbp_chr19_36142599_R GTCTCGTGGGCTCGGGCAAAGGGAGGAGAGCTCAG 60.1 0.07
mm4__chr17 28.03 Mbp_chr17_28032578_F TCGTCGGCAGCGTCGGAATTCTCCTGGGGCTCAG 59.8 0.07
mm4__chr17 28.03 Mbp_chr17_28032578_R GTCTCGTGGGCTCGGTGGGTGAGGCCAGACAAATC 59.9 0.07
mm4__chr9 113.88 Mbp_chr9_113876825_F TCGTCGGCAGCGTCTGCAATTTTCCCCTGACCCT 59.5 0.06
mm4__chr9 113.88 Mbp_chr9_113876825_R GTCTCGTGGGCTCGGAAATTGAGAAAGGAAAATATGGCCT 57.4 0.06
mm4__chr2 43.23 Mbp_chr2_43226811_F TCGTCGGCAGCGTCCTTCCCGGCCTCCCATTG 60.1 0.06
mm4__chr2 43.23 Mbp_chr2_43226811_R GTCTCGTGGGCTCGGGAGAGCTACACGCCACACC 60.4 0.06
mm4__chr9 15.55 Mbp_chr9_15553111_F Primer3: not found at this Tm N.d. 0.06
mm4__chr9 15.55 Mbp_chr9_15553111_R Primer3: not found at this Tm N.d. 0.06
mm4__chr9 87.31 Mbp_chr9_87314245_F TCGTCGGCAGCGTCGAAGGACCAGCCAAAGACCA 59.8 0.06
mm4__chr9 87.31 Mbp_chr9_87314245_R GTCTCGTGGGCTCGGTGATGTAGGGTGCACTCTGC 59.7 0.06
mm4__chr9 130.32 Mbp_chr9_130318967_F TCGTCGGCAGCGTCAGAAGGGGCTGTGGAAATGG 59.9 0.06
mm4__chr9 130.32 Mbp_chr9_130318967_R GTCTCGTGGGCTCGGTCTTGGAGGTGTCCTGAGCT 60.1 0.06
mm4__chr15 85.25 Mbp_chr15_85253808_F TCGTCGGCAGCGTCTTGAGTTGAGGAGCAGCAGG 59.9 0.06
mm4__chr15 85.25 Mbp_chr15_85253808_R GTCTCGTGGGCTCGGTTGGTGAAGAGCGTCTGTCC 59.9 0.06
mm4__chr6 33.44 Mbp_chr6_33438042_F TCGTCGGCAGCGTCGTGGTACCCTGTAACCCTGC 60.0 0.06
mm4__chr6 33.44 Mbp_chr6_33438042_R GTCTCGTGGGCTCGGCTGGTAACGTGCTTTCAGCC 59.4 0.06
mm4__chr19 41.96 Mbp_chr19_41956800_F Primer3: not found at this Tm N.d. 0.06
mm4__chr19 41.96 Mbp_chr19_41956800_R Primer3: not found at this Tm N.d. 0.06
mm4__chr11 126.45 Mbp_chr11_126452845_F TCGTCGGCAGCGTCAAGCCAGGTCTTTTGGGGAG 59.8 0.06
mm4__chr11 126.45 Mbp_chr11_126452845_R GTCTCGTGGGCTCGGTTTTGGCAGGGACCTCTCAG 59.5 0.06
mm4__chr17 77.85 Mbp_chr17_77846535_F TCGTCGGCAGCGTCCCTCCCCAGTGACCTACCAT 60.3 0.06
mm4__chr17 77.85 Mbp_chr17_77846535_R GTCTCGTGGGCTCGGAGAGGAAGCCCAGGACTTCA 60.1 0.06
mm4__chr12 122.15 Mbp_chr12_122147488_F TCGTCGGCAGCGTCCCTCGGGAGGTGTGATAAGC 59.8 0.06
mm4__chr12 122.15 Mbp_chr12_122147488_R GTCTCGTGGGCTCGGGAGCACCGACTTTCTGGACA 59.9 0.06
mm4__chr11 127.94 Mbp_chr11_127938763_F Primer3: not found at this Tm N.d. 0.06
mm4__chr11 127.94 Mbp_chr11_127938763_R Primer3: not found at this Tm N.d. 0.06
mm4__chr19 4.66 Mbp_chr19_4664754_F TCGTCGGCAGCGTCATCAGTGCACTTCCTTGGGG 59.9 0.06
mm4__chr19 4.66 Mbp_chr19_4664754_R GTCTCGTGGGCTCGGTTTGGAAGGCAGGGAACGAG 60.2 0.06
mm4__chr10 101.22 Mbp_chr10_101217493_F TCGTCGGCAGCGTCTCATCCCTAAAAGCCGGCG 60.1 0.05
mm4__chr10 101.22 Mbp_chr10_101217493_R GTCTCGTGGGCTCGGTGGAGCGGACAATTCGGG 59.7 0.05
mm4__chr21 46.10 Mbp_chr21_46098574_F Primer3: not found at this Tm N.d. 0.05
mm4__chr21 46.10 Mbp_chr21_46098574_R Primer3: not found at this Tm N.d. 0.05
mm4__chr5 176.86 Mbp_chr5_176860936_F Primer3: not found at this Tm N.d. 0.05
mm4__chr5 176.86 Mbp_chr5_176860936_R Primer3: not found at this Tm N.d. 0.05
mm4__chr1 2.20 Mbp_chr1_2197690_F TCGTCGGCAGCGTCGCACAACGTGTCACAGTTGT 59.5 0.05
mm4__chr1 2.20 Mbp_chr1_2197690_R GTCTCGTGGGCTCGGCAGTGGTGACATGGGAGGAG 59.7 0.05
mm4__chr10 8.07 Mbp_chr10_8069178_F TCGTCGGCAGCGTCCCCTCTGGTGTACTTTGTGGG 60.2 0.05
mm4__chr10 8.07 Mbp_chr10_8069178_R GTCTCGTGGGCTCGGAGAGTTTCACCTGCTGCTCC 59.9 0.05
mm4__chr17 41.82 Mbp_chr17_41822150_F TCGTCGGCAGCGTCAAGAAAGCACTCACTGGCCT 59.5 0.05
mm4__chr17 41.82 Mbp_chr17_41822150_R GTCTCGTGGGCTCGGGTCCTTTGCCCTCACTCACT 59.6 0.05
mm4__chr6 5.08 Mbp_chr6_5082828_F Primer3: not found at this Tm N.d. 0.05
mm4__chr6 5.08 Mbp_chr6_5082828_R Primer3: not found at this Tm N.d. 0.05
mm4__chr16 84.62 Mbp_chr16_84617722_F Primer3: not found at this Tm N.d. 0.05
mm4__chr16 84.62 Mbp_chr16_84617722_R Primer3: not found at this Tm N.d. 0.05
mm4__chr1 185.05 Mbp_chr1_185045466_F TCGTCGGCAGCGTCTCGCTCGCTCGCTCCTTC 62.1 0.05
mm4__chr1 185.05 Mbp_chr1_185045466_R GTCTCGTGGGCTCGGTCGGGAAATACAACAGGGGC 60.0 0.05
mm4__chr19 6.46 Mbp_chr19_6459440_F Primer3: not found at this Tm N.d. 0.05
mm4__chr19 6.46 Mbp_chr19_6459440_R Primer3: not found at this Tm N.d. 0.05
mm4__chrX 153.72 Mbp_chrX_153723851_F Primer3: not found at this Tm N.d. 0.05
mm4__chrX 153.72 Mbp_chrX_153723851_R Primer3: not found at this Tm N.d. 0.05
mm4__chr5 5.73 Mbp_chr5_5725807_F TCGTCGGCAGCGTCATCTGGGGCATGGAAATGGG 60.1 0.05
mm4__chr5 5.73 Mbp_chr5_5725807_R GTCTCGTGGGCTCGGTGCTGCTCTTGAAAAGGCAG 59.0 0.05
mm4__chr22 47.92 Mbp_chr22_47917810_F Primer3: not found at this Tm N.d. 0.04
mm4__chr22 47.92 Mbp_chr22_47917810_R Primer3: not found at this Tm N.d. 0.04
mm3__chrX 103.71 Mbp_chrX_103707301_F TCGTCGGCAGCGTCCCCACCCTATTCACAGAGCC 59.8 0.04
mm3__chrX 103.71 Mbp_chrX_103707301_R GTCTCGTGGGCTCGGCGTGGACTGAGCCAAGAGAA 59.6 0.04
mm4__chr17 1.10 Mbp_chr17_1096680_F TCGTCGGCAGCGTCTCCCTCTGGTCTCTGCTCAA 59.8 0.04
mm4__chr17 1.10 Mbp_chr17_1096680_R GTCTCGTGGGCTCGGTTCCCCAGCTCTCTCCTCC 59.9 0.04
mm3__chr2 17.88 Mbp_chr2_17879296_F TCGTCGGCAGCGTCCGTTCCTTCCCTTCCTCGAG 59.8 0.04
mm3__chr2 17.88 Mbp_chr2_17879296_R GTCTCGTGGGCTCGGAAAGGGGAGGGGAGGAATGA 59.8 0.04
mm3__chr6 42.78 Mbp_chr6_42782779_F TCGTCGGCAGCGTCGTGTGAGTGTGTTTTCCGCC 59.9 0.04
mm3__chr6 42.78 Mbp_chr6_42782779_R GTCTCGTGGGCTCGGTTTTCTTCCTCGCCCGCC 60.3 0.04
mm4__chrUn_GL000220v1 158.06 Kbp_chrUn_GL000220v1_158057_F Primer3: not found at this Tm N.d. 0.04
mm4__chrUn_GL000220v1 158.06 Kbp_chrUn_GL000220v1_158057_R Primer3: not found at this Tm N.d. 0.04
mm4__chrUn_GL000220v1 114.08 Kbp_chrUn_GL000220v1_114085_F Primer3: not found at this Tm N.d. 0.04
mm4__chrUn_GL000220v1 114.08 Kbp_chrUn_GL000220v1_114085_R Primer3: not found at this Tm N.d. 0.04
mm4__chr22_KI270733v1_random 176.02 Kbp_chr22_KI270733v1_random_176020_F Primer3: not found at this Tm N.d. 0.04
mm4__chr22_KI270733v1_random 176.02 Kbp_chr22_KI270733v1_random_176020_R Primer3: not found at this Tm N.d. 0.04
mm4__chr22_KI270733v1_random 130.94 Kbp_chr22_KI270733v1_random_130941_F Primer3: not found at this Tm N.d. 0.04
mm4__chr22_KI270733v1_random 130.94 Kbp_chr22_KI270733v1_random_130941_R Primer3: not found at this Tm N.d. 0.04
mm4__chr21 8.44 Mbp_chr21_8441883_F Primer3: not found at this Tm N.d. 0.04
mm4__chr21 8.44 Mbp_chr21_8441883_R Primer3: not found at this Tm N.d. 0.04
mm4__chr21 8.40 Mbp_chr21_8397662_F Primer3: not found at this Tm N.d. 0.04
mm4__chr21 8.40 Mbp_chr21_8397662_R Primer3: not found at this Tm N.d. 0.04
mm4__chr21 8.26 Mbp_chr21_8258848_F Primer3: not found at this Tm N.d. 0.04
mm4__chr21 8.26 Mbp_chr21_8258848_R Primer3: not found at this Tm N.d. 0.04
mm4__chr21 8.21 Mbp_chr21_8214625_F Primer3: not found at this Tm N.d. 0.04
mm4__chr21 8.21 Mbp_chr21_8214625_R Primer3: not found at this Tm N.d. 0.04
mm4__chr7 6.66 Mbp_chr7_6656105_F TCGTCGGCAGCGTCGGTAGGAGGCACGGTAGTTG 59.8 0.04
mm4__chr7 6.66 Mbp_chr7_6656105_R GTCTCGTGGGCTCGGCACCCCAATGCCTCCCTG 60.0 0.04
mm4__chr2 10.50 Mbp_chr2_10501046_F TCGTCGGCAGCGTCACAAACACACAGAGACGCCA 60.1 0.04
mm4__chr2 10.50 Mbp_chr2_10501046_R GTCTCGTGGGCTCGGGGTAAAAGAGGGGCGTTGGA 59.9 0.04
mm4__chr18 79.77 Mbp_chr18_79771489_F Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771489_R Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771472_F Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771472_R Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771438_F Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771438_R Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771421_F Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771421_R Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771353_F Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771353_R Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771319_F TCGTCGGCAGCGTCCATCCTCCCCTCCACGCC 61.8 0.04
mm4__chr18 79.77 Mbp_chr18_79771319_R GTCTCGTGGGCTCGGATGTCATGGAGGGGAGGCT 59.9 0.04
mm4__chr18 79.77 Mbp_chr18_79771302_F TCGTCGGCAGCGTCCATCCTCCCCTCCACGCC 61.8 0.04
mm4__chr18 79.77 Mbp_chr18_79771302_R GTCTCGTGGGCTCGGATGTCATGGAGGGGAGGCT 59.9 0.04
mm4__chr18 79.77 Mbp_chr18_79771268_F Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771268_R Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771234_F Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771234_R Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771149_F Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771149_R Primer3: not found at this Tm N.d. 0.04
mm4__chr18 79.77 Mbp_chr18_79771132_F TCGTCGGCAGCGTCGTCCTCCCCTCCATGCCA 60.6 0.04
mm4__chr18 79.77 Mbp_chr18_79771132_R GTCTCGTGGGCTCGGCTGTGGAGGGGAGGACGG 61.0 0.04
mm4__chr18 79.77 Mbp_chr18_79771115_F TCGTCGGCAGCGTCGTCCTCCCCTCCATGCCA 60.6 0.04
mm4__chr18 79.77 Mbp_chr18_79771115_R GTCTCGTGGGCTCGGCTGTGGAGGGGAGGACGG 61.0 0.04
mm4__chr18 79.77 Mbp_chr18_79771098_F TCGTCGGCAGCGTCGTCCTCCCCTCCATGCCA 60.6 0.04
mm4__chr18 79.77 Mbp_chr18_79771098_R GTCTCGTGGGCTCGGCTGTGGAGGGGAGGACGG 61.0 0.04
mm4__chr18 79.77 Mbp_chr18_79771064_F TCGTCGGCAGCGTCTCGGAATCCAAAGGCGCAG 60.7 0.04
mm4__chr18 79.77 Mbp_chr18_79771064_R GTCTCGTGGGCTCGGTGGAGGGGAGGCTGGCAT 62.7 0.04
mm4__chr18 79.77 Mbp_chr18_79770996_F TCGTCGGCAGCGTCCTGGGGTCGGAATCCAAAGG 60.3 0.04
mm4__chr18 79.77 Mbp_chr18_79770996_R GTCTCGTGGGCTCGGCCTGTGTGGAGGGGAGGA 59.8 0.04
mm4__chr9 21.11 Mbp_chr9_21105861_F Primer3: not found at this Tm N.d. 0.04
mm4__chr9 21.11 Mbp_chr9_21105861_R Primer3: not found at this Tm N.d. 0.04
mm4__chr7 129.99 Mbp_chr7_129990408_F TCGTCGGCAGCGTCATGAGAGGCCTTGAACTGGG 59.3 0.04
mm4__chr7 129.99 Mbp_chr7_129990408_R GTCTCGTGGGCTCGGGCCAGAGATGAATGGTGGCT 60.1 0.04
mm4__chr8 12.44 Mbp_chr8_12436911_F TCGTCGGCAGCGTCCTTAGGACGGGCCCACTTG 60.0 0.04
mm4__chr8 12.44 Mbp_chr8_12436911_R GTCTCGTGGGCTCGGTCCCTAAGCTCCGCCTCC 60.4 0.04
mm4__chr10 3.54 Mbp_chr10_3536128_F TCGTCGGCAGCGTCGCTGTCCTAGAAGCCTCACC 59.8 0.04
mm4__chr10 3.54 Mbp_chr10_3536128_R GTCTCGTGGGCTCGGTGGGTTTCTGCAGCAGGAAT 59.8 0.04
mm4__chr1 156.59 Mbp_chr1_156591622_F TCGTCGGCAGCGTCGTGTGGCGATCTGTAAACGC 59.9 0.04
mm4__chr1 156.59 Mbp_chr1_156591622_R GTCTCGTGGGCTCGGGTCTTGGGGTTCTAGCAGCC 60.3 0.04
mm4__chr19 56.12 Mbp_chr19_56120940_F Primer3: not found at this Tm N.d. 0.04
mm4__chr19 56.12 Mbp_chr19_56120940_R Primer3: not found at this Tm N.d. 0.04
mm3__chr2 27.12 Mbp_chr2_27123662_F TCGTCGGCAGCGTCTTAAGGGAGCTGGAGATGCC 59.1 0.04
mm3__chr2 27.12 Mbp_chr2_27123662_R GTCTCGTGGGCTCGGATCTAACCCGAGTGACCTGC 59.1 0.04
mm4__chr4 38.01 Mbp_chr4_38014537_F TCGTCGGCAGCGTCCTCTCTCTCCTCAGGTGCCT 60.0 0.04
mm4__chr4 38.01 Mbp_chr4_38014537_R GTCTCGTGGGCTCGGGCAGAAGAGCACCTCGAACT 60.0 0.04
mm4__chr2 5.67 Mbp_chr2_5673540_F Primer3: not found at this Tm N.d. 0.04
mm4__chr2 5.67 Mbp_chr2_5673540_R Primer3: not found at this Tm N.d. 0.04
mm4__chr18 58.69 Mbp_chr18_58692460_F Primer3: not found at this Tm N.d. 0.04
mm4__chr18 58.69 Mbp_chr18_58692460_R Primer3: not found at this Tm N.d. 0.04
mm4__chr19 6.53 Mbp_chr19_6531163_F Primer3: not found at this Tm N.d. 0.04
mm4__chr19 6.53 Mbp_chr19_6531163_R Primer3: not found at this Tm N.d. 0.04
mm4__chr20 51.49 Mbp_chr20_51492633_F TCGTCGGCAGCGTCCCACCAGCCAAGATAAGGGG 60.1 0.03
mm4__chr20 51.49 Mbp_chr20_51492633_R GTCTCGTGGGCTCGGGGGAGCCCATTAGCCAGTG 60.1 0.03
mm4__chr1 43.58 Mbp_chr1_43584522_F TCGTCGGCAGCGTCGACAGCCCAGCCCAGAGT 61.3 0.03
mm4__chr1 43.58 Mbp_chr1_43584522_R GTCTCGTGGGCTCGGCATGTCTGCCTTCCCGCTAA 60.1 0.03
mm4__chr20 36.75 Mbp_chr20_36745880_F Primer3: not found at this Tm N.d. 0.03
mm4__chr20 36.75 Mbp_chr20_36745880_R Primer3: not found at this Tm N.d. 0.03
mm3__chr8 119.21 Mbp_chr8_119208665_F Primer3: not found at this Tm N.d. 0.03
mm3__chr8 119.21 Mbp_chr8_119208665_R Primer3: not found at this Tm N.d. 0.03
mm4__chr2 239.03 Mbp_chr2_239026890_F Primer3: not found at this Tm N.d. 0.03
mm4__chr2 239.03 Mbp_chr2_239026890_R Primer3: not found at this Tm N.d. 0.03
mm2__chr10 24.95 Mbp_chr10_24952594_F TCGTCGGCAGCGTCTTTGCATTTACCACGACGCC 59.7 0.03
mm2__chr10 24.95 Mbp_chr10_24952594_R GTCTCGTGGGCTCGGCCTTCGCCCTCCCTGCTC 62.1 0.03
mm4__chr11 6.41 Mbp_chr11_6410878_F TCGTCGGCAGCGTCACTCTCCAGGGGCTCAGG 59.9 0.03
mm4__chr11 6.41 Mbp_chr11_6410878_R GTCTCGTGGGCTCGGCAGCACTCAAGAGCAGGGG 60.3 0.03
mm4__chrX 37.35 Mbp_chrX_37350056_F TCGTCGGCAGCGTCGTTTTGTAGGCAGTGGCGG 59.4 0.03
mm4__chrX 37.35 Mbp_chrX_37350056_R GTCTCGTGGGCTCGGAAGCAGGAAGACTGTTTCTCTT 57.5 0.03
mm4__chr11 119.98 Mbp_chr11_119978849_F Primer3: not found at this Tm N.d. 0.03
mm4__chr11 119.98 Mbp_chr11_119978849_R Primer3: not found at this Tm N.d. 0.03
mm4__chr2 264.54 Kbp_chr2_264540_F TCGTCGGCAGCGTCGCCCCGTCCCCTATAGGTAA 60.1 0.03
mm4__chr2 264.54 Kbp_chr2_264540_R GTCTCGTGGGCTCGGGGGTGTGTGTGTGTGGTGG 61.1 0.03
mm4__chr7 67.85 Mbp_chr7_67852850_F Primer3: not found at this Tm N.d. 0.03
mm4__chr7 67.85 Mbp_chr7_67852850_R Primer3: not found at this Tm N.d. 0.03
mm3__chr7 75.99 Mbp_chr7_75988756_F Primer3: not found at this Tm N.d. 0.02
mm3__chr7 75.99 Mbp_chr7_75988756_R Primer3: not found at this Tm N.d. 0.02
mm4__chr9 94.61 Mbp_chr9_94614145_F Primer3: not found at this Tm N.d. 0.02
mm4__chr9 94.61 Mbp_chr9_94614145_R Primer3: not found at this Tm N.d. 0.02
mm4__chr9 89.50 Mbp_chr9_89497629_F TCGTCGGCAGCGTCAGGGACTGGGGAGGCAAC 60.9 0.02
mm4__chr9 89.50 Mbp_chr9_89497629_R GTCTCGTGGGCTCGGCCCCTGGACCTCTCTGCTAT 60.1 0.02
mm4__chr2 32.01 Mbp_chr2_32010068_F Primer3: not found at this Tm N.d. 0.02
mm4__chr2 32.01 Mbp_chr2_32010068_R Primer3: not found at this Tm N.d. 0.02
mm4__chr7 102.32 Mbp_chr7_102318012_F TCGTCGGCAGCGTCCTGCAGGGGAGGGAGAATTG 60.1 0.02
mm4__chr7 102.32 Mbp_chr7_102318012_R GTCTCGTGGGCTCGGCTTCCTTGAAAGCACACCGC 60.0 0.02
mm4__chr15 55.74 Mbp_chr15_55743497_F TCGTCGGCAGCGTCCCGCGGCTAGTTGAGGATT 59.8 0.02
mm4__chr15 55.74 Mbp_chr15_55743497_R GTCTCGTGGGCTCGGGTAGGAGGGTGTAGGAGGGG 60.1 0.02
mm3__chr2 85.44 Mbp_chr2_85437850_F TCGTCGGCAGCGTCCATCCCACTCCGCACCCC 62.4 0.02
mm3__chr2 85.44 Mbp_chr2_85437850_R GTCTCGTGGGCTCGGCCAGTGTCTTCAGTGCCAGT 59.8 0.02
mm3__chr10 70.48 Mbp_chr10_70478534_F TCGTCGGCAGCGTCGGTGTCTTCCAGAAGGAGCC 60.0 0.02
mm3__chr10 70.48 Mbp_chr10_70478534_R GTCTCGTGGGCTCGGCGCGAGGAGGAAAAGTTTGG 59.4 0.02
mm4__chr17 50.22 Mbp_chr17_50220935_F TCGTCGGCAGCGTCCAGGTGCCTCATCCTTCCTC 59.8 0.02
mm4__chr17 50.22 Mbp_chr17_50220935_R GTCTCGTGGGCTCGGCGTTAGCTAGTCCTGAGCGG 59.9 0.02
mm4__chr9 113.36 Mbp_chr9_113355504_F Primer3: not found at this Tm N.d. 0.02
mm4__chr9 113.36 Mbp_chr9_113355504_R Primer3: not found at this Tm N.d. 0.02
mm3__chr19 38.51 Mbp_chr19_38512045_F TCGTCGGCAGCGTCTGGGGTGGATGTAGAGGGAG 60.0 0.02
mm3__chr19 38.51 Mbp_chr19_38512045_R GTCTCGTGGGCTCGGGGCCAGGATGTGAAGACAGT 59.6 0.02
mm4__chr8 142.83 Mbp_chr8_142834791_F TCGTCGGCAGCGTCCCCGAAAGTTCCAGAAAGCG 59.4 0.02
mm4__chr8 142.83 Mbp_chr8_142834791_R GTCTCGTGGGCTCGGGAAACTGACCTGTGCCCCTC 60.6 0.02
mm3__chr20 64.01 Mbp_chr20_64007390_F Primer3: not found at this Tm N.d. 0.02
mm3__chr20 64.01 Mbp_chr20_64007390_R Primer3: not found at this Tm N.d. 0.02
mm4__chr7 131.59 Mbp_chr7_131592980_F TCGTCGGCAGCGTCAGCAGTTTGGCCAAGGGTAG 60.2 0.02
mm4__chr7 131.59 Mbp_chr7_131592980_R GTCTCGTGGGCTCGGAGCTCCATCACCAGCTGAAC 60.0 0.02
mm3__chrX 153.69 Mbp_chrX_153694898_F TCGTCGGCAGCGTCCACCATGGCTGAGGTAAGGC 60.7 0.02
mm3__chrX 153.69 Mbp_chrX_153694898_R GTCTCGTGGGCTCGGAGCAGGCTGGGTTGAATGTT 60.1 0.02
mm4__chr22 47.97 Mbp_chr22_47965661_F TCGTCGGCAGCGTCGCTGGCATGGTCTCTCTTGT 60.0 0.02
mm4__chr22 47.97 Mbp_chr22_47965661_R GTCTCGTGGGCTCGGACCTTCCATTGCCAAACCCT 59.8 0.02
mm4__chr22 47.97 Mbp_chr22_47965643_F TCGTCGGCAGCGTCGCTGGCATGGTCTCTCTTGT 60.0 0.02
mm4__chr22 47.97 Mbp_chr22_47965643_R GTCTCGTGGGCTCGGACCTTCCATTGCCAAACCCT 59.8 0.02
mm4__chr20 62.96 Mbp_chr20_62959057_F TCGTCGGCAGCGTCATGAACGAATCGGCAACCAC 59.1 0.02
mm4__chr20 62.96 Mbp_chr20_62959057_R GTCTCGTGGGCTCGGTCCTCACAGCTTCAGCCTTG 59.9 0.02
mm4__chr16_KI270728v1_random 1.14 Mbp_chr16_KI270728v1_random_1141254_F TCGTCGGCAGCGTCAGCAGGCTGGGTTGAATGTT 60.1 0.02
mm4__chr16_KI270728v1_random 1.14 Mbp_chr16_KI270728v1_random_1141254_R GTCTCGTGGGCTCGGCCATGGCTGAGGTAAGGCTC 60.1 0.02
mm4__chr16 33.98 Mbp_chr16_33982366_F TCGTCGGCAGCGTCCACCATGGCTGAGGTAAGGC 60.7 0.02
mm4__chr16 33.98 Mbp_chr16_33982366_R GTCTCGTGGGCTCGGAGCAGGCTGGGTTGAATGTT 60.1 0.02
mm4__chr16 32.88 Mbp_chr16_32879145_F TCGTCGGCAGCGTCAGCAGGCTGGGTTGAATGTT 60.1 0.02
mm4__chr16 32.88 Mbp_chr16_32879145_R GTCTCGTGGGCTCGGCCATGGCTGAGGTAAGGCTC 60.1 0.02
mm3__chr1 108.75 Mbp_chr1_108747063_F TCGTCGGCAGCGTCCTTCTCGTTGGCGTCGGG 60.8 0.02
mm3__chr1 108.75 Mbp_chr1_108747063_R GTCTCGTGGGCTCGGAGCGGAGAAGAGTGGGGAG 60.6 0.02
mm4__chr17 4.54 Mbp_chr17_4544987_F TCGTCGGCAGCGTCCAGGCACACAACATGGGGA 60.2 0.01
mm4__chr17 4.54 Mbp_chr17_4544987_R GTCTCGTGGGCTCGGTTCCGGGAACAGCTGATGAC 60.0 0.01
mm2__chr7 155.35 Mbp_chr7_155350668_F Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350668_R Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350596_F Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350596_R Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350524_F Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350524_R Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350452_F Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350452_R Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350416_F Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350416_R Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350236_F Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350236_R Primer3: not found at this Tm N.d. 0.01
mm4__chr15 45.21 Mbp_chr15_45207096_F Primer3: not found at this Tm N.d. 0.01
mm4__chr15 45.21 Mbp_chr15_45207096_R Primer3: not found at this Tm N.d. 0.01
mm4__chr15 90.96 Mbp_chr15_90957303_F TCGTCGGCAGCGTCCATTGTGGGCCCAGAATGTG 59.4 0.01
mm4__chr15 90.96 Mbp_chr15_90957303_R GTCTCGTGGGCTCGGGCCAGAGGCCGGTAGAAGG 62.0 0.01
mm4__chr9 134.58 Mbp_chr9_134579870_F Primer3: not found at this Tm N.d. 0.01
mm4__chr9 134.58 Mbp_chr9_134579870_R Primer3: not found at this Tm N.d. 0.01
mm4__chr9 134.58 Mbp_chr9_134579785_F Primer3: not found at this Tm N.d. 0.01
mm4__chr9 134.58 Mbp_chr9_134579785_R Primer3: not found at this Tm N.d. 0.01
mm4__chr13 39.18 Mbp_chr13_39177432_F Primer3: not found at this Tm N.d. 0.01
mm4__chr13 39.18 Mbp_chr13_39177432_R Primer3: not found at this Tm N.d. 0.01
mm4__chr5 132.49 Mbp_chr5_132490001_F TCGTCGGCAGCGTCACAACGCTAAGACCAGGACG 60.0 0.01
mm4__chr5 132.49 Mbp_chr5_132490001_R GTCTCGTGGGCTCGGTGTTCCTTGGGCCACTTCTC 59.8 0.01
mm4__chr16 85.69 Mbp_chr16_85692700_F TCGTCGGCAGCGTCGGAACCAGAGTCAACAGACAGA 59.6 0.01
mm4__chr16 85.69 Mbp_chr16_85692700_R GTCTCGTGGGCTCGGAGGGGAGGCGTTTTACAGAA 58.9 0.01
mm4__chr14 68.92 Mbp_chr14_68915246_F Primer3: not found at this Tm N.d. 0.01
mm4__chr14 68.92 Mbp_chr14_68915246_R Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350308_F Primer3: not found at this Tm N.d. 0.01
mm2__chr7 155.35 Mbp_chr7_155350308_R Primer3: not found at this Tm N.d. 0.01
mm4__chr19 13.02 Mbp_chr19_13024952_F TCGTCGGCAGCGTCACGCTGCTTTTCTCCTTCTCT 59.6 0.01
mm4__chr19 13.02 Mbp_chr19_13024952_R GTCTCGTGGGCTCGGCGATGAACGGGTGGAACTCA 60.0 0.01
mm4__chr10 43.07 Mbp_chr10_43072967_F TCGTCGGCAGCGTCTGGTGCTGAGCGAAAAGGAT 59.9 0.01
mm4__chr10 43.07 Mbp_chr10_43072967_R GTCTCGTGGGCTCGGTGAATTTCCTTCCCTCGCCC 60.0 0.01
mm4__chr2 5.24 Mbp_chr2_5236303_F TCGTCGGCAGCGTCGCTGTGGAAGACTCTCCTCG 59.8 0.01
mm4__chr2 5.24 Mbp_chr2_5236303_R GTCTCGTGGGCTCGGGGGAAGCAGAACTTGGCAGT 60.5 0.01
mm4__chr2 10.28 Mbp_chr2_10277916_F Primer3: not found at this Tm N.d. 0.01
mm4__chr2 10.28 Mbp_chr2_10277916_R Primer3: not found at this Tm N.d. 0.01
mm4__chr17 68.51 Mbp_chr17_68512843_F TCGTCGGCAGCGTCGTTGGCTTTGGTGCGGGG 61.9 0.01
mm4__chr17 68.51 Mbp_chr17_68512843_R GTCTCGTGGGCTCGGGAGGGTGAGCGAAGAGGC 59.8 0.01
mm4__chr3 128.18 Mbp_chr3_128175138_F TCGTCGGCAGCGTCTCATCATCGGCATTCTCTCTTT 57.5 0.01
mm4__chr3 128.18 Mbp_chr3_128175138_R GTCTCGTGGGCTCGGGGGTTTGCAGCAGTACTAGT 57.5 0.01
mm4__chr8 1.97 Mbp_chr8_1974091_F TCGTCGGCAGCGTCTAGGCGGGAGGGGAGAAAAG 60.9 0.01
mm4__chr8 1.97 Mbp_chr8_1974091_R GTCTCGTGGGCTCGGCCCCTCCTCTCCCGGTCC 62.8 0.01
mm4__chr1 178.76 Mbp_chr1_178758532_F Primer3: not found at this Tm N.d. 0.01
mm4__chr1 178.76 Mbp_chr1_178758532_R Primer3: not found at this Tm N.d. 0.01
mm4__chr7 50.34 Mbp_chr7_50336591_F TCGTCGGCAGCGTCGTCAGATAGCTCGCCCTTGG 60.2 0.01
mm4__chr7 50.34 Mbp_chr7_50336591_R GTCTCGTGGGCTCGGCTGAGATGCCCTTCTCTGCC 60.1 0.01
mm4__chr6 132.70 Mbp_chr6_132698647_F TCGTCGGCAGCGTCTGATCTTGGTGGCTCTGCAG 60.0 0.01
mm4__chr6 132.70 Mbp_chr6_132698647_R GTCTCGTGGGCTCGGAAAGCAAACAGAGAGGCCCA 59.8 0.01
mm4__chr5 111.07 Mbp_chr5_111073408_F TCGTCGGCAGCGTCCTCTCGACTTGTGTTCCCCG 60.3 0.01
mm4__chr5 111.07 Mbp_chr5_111073408_R GTCTCGTGGGCTCGGTGCTCATAGGCGGCAAAGTT 60.3 0.01
mm4__chrY 668.17 Kbp_chrY_668169_F Primer3: not found at this Tm N.d. 0.01
mm4__chrY 668.17 Kbp_chrY_668169_R Primer3: not found at this Tm N.d. 0.01
mm4__chr9 133.91 Mbp_chr9_133914760_F Primer3: not found at this Tm N.d. 0.01
mm4__chr9 133.91 Mbp_chr9_133914760_R Primer3: not found at this Tm N.d. 0.01
mm4__chr13 112.80 Mbp_chr13_112803722_F Primer3: not found at this Tm N.d. 0.01
mm4__chr13 112.80 Mbp_chr13_112803722_R Primer3: not found at this Tm N.d. 0.01
mm4__chr6 43.94 Mbp_chr6_43939721_F TCGTCGGCAGCGTCATTGGGCACTGAATGGGTCC 60.3 0.01
mm4__chr6 43.94 Mbp_chr6_43939721_R GTCTCGTGGGCTCGGCAGGCATAACCCTGCTCAGG 60.4 0.01
mm4__chr11 116.70 Mbp_chr11_116703481_F Primer3: not found at this Tm N.d. 0.01
mm4__chr11 116.70 Mbp_chr11_116703481_R Primer3: not found at this Tm N.d. 0.01
mm4__chr11 78.03 Mbp_chr11_78030882_F TCGTCGGCAGCGTCCAAGGCTCCCTCTGTGAGTC 59.7 0.01
mm4__chr11 78.03 Mbp_chr11_78030882_R GTCTCGTGGGCTCGGTCTCAAGCAGGTATGGAGCAG 59.5 0.01
mm4__chr1 206.12 Mbp_chr1_206117192_F Primer3: not found at this Tm N.d. 0.01
mm4__chr1 206.12 Mbp_chr1_206117192_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 2.39 Mbp_chr1_2392206_F Primer3: not found at this Tm N.d. 0.01
mm4__chr1 2.39 Mbp_chr1_2392206_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 39.58 Mbp_chr1_39576519_F Primer3: not found at this Tm N.d. 0.01
mm4__chr1 39.58 Mbp_chr1_39576519_R Primer3: not found at this Tm N.d. 0.01
mm4__chr17 82.06 Mbp_chr17_82058578_F TCGTCGGCAGCGTCCAAGCCAGATGCCTCTCCC 60.1 0.01
mm4__chr17 82.06 Mbp_chr17_82058578_R GTCTCGTGGGCTCGGGTTTGCAACGTGGCTCAAGG 60.5 0.01
mm4__chr19 43.41 Mbp_chr19_43408444_F TCGTCGGCAGCGTCCTATTCGAGGGGTGGCTGTG 60.1 0.01
mm4__chr19 43.41 Mbp_chr19_43408444_R GTCTCGTGGGCTCGGTTTAAGAGGAGGAGGCGTGC 59.7 0.01
mm4__chr2 231.56 Mbp_chr2_231555245_F Primer3: not found at this Tm N.d. 0.01
mm4__chr2 231.56 Mbp_chr2_231555245_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 6.94 Mbp_chr1_6943156_F TCGTCGGCAGCGTCGACCCCATTAGCAGCGGTTA 59.8 0.01
mm4__chr1 6.94 Mbp_chr1_6943156_R GTCTCGTGGGCTCGGCAGGAACCATGGTGTCCTGG 60.3 0.01
mm4__chr8 2.02 Mbp_chr8_2023960_F TCGTCGGCAGCGTCCTCTGCAGGGAGGAGGAGG 60.7 0.01
mm4__chr8 2.02 Mbp_chr8_2023960_R GTCTCGTGGGCTCGGCTGAGAACAGCTCCCGATGC 60.8 0.01
mm2__chr7 75.50 Mbp_chr7_75499258_F TCGTCGGCAGCGTCTGACCTCTCCCTCTCTGTGT 59.2 0.01
mm2__chr7 75.50 Mbp_chr7_75499258_R GTCTCGTGGGCTCGGAAACCACTCACAGAGCCAGG 59.8 0.01
mm2__chr7 102.66 Mbp_chr7_102657578_F TCGTCGGCAGCGTCTGACCTCTCCCTCTCTGTGT 59.2 0.01
mm2__chr7 102.66 Mbp_chr7_102657578_R GTCTCGTGGGCTCGGAAACCACTCACAGAGCCAGG 59.8 0.01
mm2__chr7 102.56 Mbp_chr7_102558494_F TCGTCGGCAGCGTCTGACCTCTCCCTCTCTGTGT 59.2 0.01
mm2__chr7 102.56 Mbp_chr7_102558494_R GTCTCGTGGGCTCGGAAACCACTCACAGAGCCAGG 59.8 0.01
mm4__chr20 22.26 Mbp_chr20_22263465_F TCGTCGGCAGCGTCTCAGAGTCCTGTCTTTTGGCT 58.9 0.01
mm4__chr20 22.26 Mbp_chr20_22263465_R GTCTCGTGGGCTCGGCTGTTCTGGGGAAGAGCTGG 60.0 0.01
mm4__chr17 2.37 Mbp_chr17_2365370_F TCGTCGGCAGCGTCGAGTGTCCCGAGCTTCATCC 60.1 0.01
mm4__chr17 2.37 Mbp_chr17_2365370_R GTCTCGTGGGCTCGGGTGTTAACCTGGCCTGGTCA 59.8 0.01
mm4__chr13 97.77 Mbp_chr13_97774447_F Primer3: not found at this Tm N.d. 0.01
mm4__chr13 97.77 Mbp_chr13_97774447_R Primer3: not found at this Tm N.d. 0.01
mm4__chr16 27.32 Mbp_chr16_27318777_F TCGTCGGCAGCGTCGAGTTGGAGGAAGTGGGTCC 59.6 0.01
mm4__chr16 27.32 Mbp_chr16_27318777_R GTCTCGTGGGCTCGGAACTTTGCTTTGCAGGCGAG 59.9 0.01
mm4__chr9 136.70 Mbp_chr9_136696887_F Primer3: not found at this Tm N.d. 0.01
mm4__chr9 136.70 Mbp_chr9_136696887_R Primer3: not found at this Tm N.d. 0.01
mm4__chr5 55.93 Mbp_chr5_55928159_F Primer3: not found at this Tm N.d. 0.01
mm4__chr5 55.93 Mbp_chr5_55928159_R Primer3: not found at this Tm N.d. 0.01
mm4__chr3 194.28 Mbp_chr3_194277433_F TCGTCGGCAGCGTCCATCACCCACAGGCAGCC 60.7 0.01
mm4__chr3 194.28 Mbp_chr3_194277433_R GTCTCGTGGGCTCGGACAGCTGTCCCTGAAACACA 59.4 0.01
mm4__chr16 9.18 Mbp_chr16_9177393_F Primer3: not found at this Tm N.d. 0.01
mm4__chr16 9.18 Mbp_chr16_9177393_R Primer3: not found at this Tm N.d. 0.01
mm4__chr14 24.28 Mbp_chr14_24281098_F Primer3: not found at this Tm N.d. 0.01
mm4__chr14 24.28 Mbp_chr14_24281098_R Primer3: not found at this Tm N.d. 0.01
mm4__chr17 63.70 Mbp_chr17_63700367_F TCGTCGGCAGCGTCCCGCCTTTGTCTGTCCCC 60.3 0.01
mm4__chr17 63.70 Mbp_chr17_63700367_R GTCTCGTGGGCTCGGCCACCGTTGCTTCCCCAC 60.9 0.01
mm4__chr6 39.19 Mbp_chr6_39189897_F TCGTCGGCAGCGTCGTGGGCCAAGAGCAATTTGG 60.0 0.01
mm4__chr6 39.19 Mbp_chr6_39189897_R GTCTCGTGGGCTCGGGTCTTCTCCTTCCCTGTGCC 60.0 0.01
mm4__chr16 1.15 Mbp_chr16_1152701_F Primer3: not found at this Tm N.d. 0.01
mm4__chr16 1.15 Mbp_chr16_1152701_R Primer3: not found at this Tm N.d. 0.01
mm4__chr1 23.53 Mbp_chr1_23534911_F Primer3: not found at this Tm N.d. 0.01
mm4__chr1 23.53 Mbp_chr1_23534911_R Primer3: not found at this Tm N.d. 0.01
mm4__chr19 782.06 Kbp_chr19_782056_F TCGTCGGCAGCGTCACAGACCAGGCAGCTGAATC 60.0 0.01
mm4__chr19 782.06 Kbp_chr19_782056_R GTCTCGTGGGCTCGGGTGTGGCTCACAGAGGGAC 60.0 0.01
mm4__chr20 61.84 Mbp_chr20_61835986_F TCGTCGGCAGCGTCTGCCAGAAGGACCCTGACTA 59.8 0.01
mm4__chr20 61.84 Mbp_chr20_61835986_R GTCTCGTGGGCTCGGTGGAAACACAGATGGAGCCC 59.9 0.01
mm4__chr5 132.67 Mbp_chr5_132667977_F TCGTCGGCAGCGTCATGACTCAAGAGCCCACAGC 60.0 0.01
mm4__chr5 132.67 Mbp_chr5_132667977_R GTCTCGTGGGCTCGGCACTGAAGAGAGGGCTCCCA 60.9 0.01
mm3__chr5 149.42 Mbp_chr5_149424539_F TCGTCGGCAGCGTCACTCAAGGGAAGAGCTGCTG 59.6 0.01
mm3__chr5 149.42 Mbp_chr5_149424539_R GTCTCGTGGGCTCGGTCCTGGAGGAGGAGTCAGTG 59.9 0.01
mm4__chr2 176.10 Mbp_chr2_176099645_F TCGTCGGCAGCGTCTCGCTCAACCTGATTTGGCT 59.9 0.00
mm4__chr2 176.10 Mbp_chr2_176099645_R GTCTCGTGGGCTCGGTCATTTCAGCACCGCCTACA 59.6 0.00
mm4__chr3 195.76 Mbp_chr3_195763320_F TCGTCGGCAGCGTCATTTACTCCGGGACAGCTGC 60.1 0.00
mm4__chr3 195.76 Mbp_chr3_195763320_R GTCTCGTGGGCTCGGACATACTGAGGCCGAGGAGA 59.7 0.00
mm4__chr4 181.92 Mbp_chr4_181915660_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 181.92 Mbp_chr4_181915660_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 9.38 Mbp_chr1_9383327_F TCGTCGGCAGCGTCGACCTTGTGCAGAGCAGATG 58.9 0.00
mm4__chr1 9.38 Mbp_chr1_9383327_R GTCTCGTGGGCTCGGTTCCTTGCTGTCAGCCTTCC 60.2 0.00
mm4__chr11 44.67 Mbp_chr11_44668950_F TCGTCGGCAGCGTCTTGAACAAAGCAAGCTCCCT 57.9 0.00
mm4__chr11 44.67 Mbp_chr11_44668950_R GTCTCGTGGGCTCGGCATGTCGCCTGGGAAGGTTA 59.7 0.00
mm4__chr17 17.79 Mbp_chr17_17792869_F TCGTCGGCAGCGTCCTGAGGCAAAAGGAAGTGGC 59.4 0.00
mm4__chr17 17.79 Mbp_chr17_17792869_R GTCTCGTGGGCTCGGGCATGACTCGGGCTGGTTAT 60.1 0.00
mm4__chr11 64.83 Mbp_chr11_64832786_F TCGTCGGCAGCGTCAAAGGCAAGCATGGGAGGG 60.3 0.00
mm4__chr11 64.83 Mbp_chr11_64832786_R GTCTCGTGGGCTCGGATGAGGGCTTTCTGTCGGTG 60.0 0.00
mm4__chr1 17.67 Mbp_chr1_17672194_F TCGTCGGCAGCGTCACACCAACAGCCCCTTGATT 59.8 0.00
mm4__chr1 17.67 Mbp_chr1_17672194_R GTCTCGTGGGCTCGGATGCCGCATCACACTCGTAT 59.8 0.00
mm4__chr19 2.50 Mbp_chr19_2497328_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 2.50 Mbp_chr19_2497328_R Primer3: not found at this Tm N.d. 0.00
mm4__chr8 37.97 Mbp_chr8_37965323_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 37.97 Mbp_chr8_37965323_R Primer3: not found at this Tm N.d. 0.00
mm4__chr16 57.52 Mbp_chr16_57517076_F TCGTCGGCAGCGTCCCCTCTCTTGTTGTCCAGGG 59.6 0.00
mm4__chr16 57.52 Mbp_chr16_57517076_R GTCTCGTGGGCTCGGAAAGCAGGAGGGGAGTGTGA 60.7 0.00
mm4__chr19 3.64 Mbp_chr19_3643633_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 3.64 Mbp_chr19_3643633_R Primer3: not found at this Tm N.d. 0.00
mm4__chrX 50.72 Mbp_chrX_50723334_F Primer3: not found at this Tm N.d. 0.00
mm4__chrX 50.72 Mbp_chrX_50723334_R Primer3: not found at this Tm N.d. 0.00
mm4__chr16 88.24 Mbp_chr16_88240818_F TCGTCGGCAGCGTCAGCCCCTTGTGATGGAAAAGT 59.8 0.00
mm4__chr16 88.24 Mbp_chr16_88240818_R GTCTCGTGGGCTCGGCAGGTGGCTTGTGTGTCTCT 59.8 0.00
mm4__chr16 50.63 Mbp_chr16_50633524_F TCGTCGGCAGCGTCAGAGGGGCAAGAAACAAGCC 60.5 0.00
mm4__chr16 50.63 Mbp_chr16_50633524_R GTCTCGTGGGCTCGGCGGTGCTTGTGCTTCTTGTG 60.3 0.00
mm4__chr22_KI270734v1_random 135.21 Kbp_chr22_KI270734v1_random_135212_F TCGTCGGCAGCGTCTGAAGCCCTCAGACTGCAG 59.3 0.00
mm4__chr22_KI270734v1_random 135.21 Kbp_chr22_KI270734v1_random_135212_R GTCTCGTGGGCTCGGCCAGGGGATTCCAGAAGGTG 59.7 0.00
mm4__chr22 20.32 Mbp_chr22_20316446_F TCGTCGGCAGCGTCGTCTCCAGGGGACTCCAGAA 60.2 0.00
mm4__chr22 20.32 Mbp_chr22_20316446_R GTCTCGTGGGCTCGGGAAGCCCTCAGACTGCAGC 60.7 0.00
mm4__chr22 18.91 Mbp_chr22_18909941_F TCGTCGGCAGCGTCTGAAGCCCTCAGACTGCAG 59.3 0.00
mm4__chr22 18.91 Mbp_chr22_18909941_R GTCTCGTGGGCTCGGCCAGGGGATTCCAGAAGGTG 59.7 0.00
mm4__chr2 45.26 Mbp_chr2_45262933_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 45.26 Mbp_chr2_45262933_R Primer3: not found at this Tm N.d. 0.00
mm4__chr17 17.28 Mbp_chr17_17280911_F TCGTCGGCAGCGTCAAACTGTCAAGCAAAGCGCC 60.2 0.00
mm4__chr17 17.28 Mbp_chr17_17280911_R GTCTCGTGGGCTCGGCCGAGTGTAGGCAGCCCC 62.1 0.00
mm4__chr3 179.15 Mbp_chr3_179148475_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 179.15 Mbp_chr3_179148475_R Primer3: not found at this Tm N.d. 0.00
mm4__chr15 32.39 Mbp_chr15_32388319_F TCGTCGGCAGCGTCGACAGAGAGAACAGGCCACC 60.0 0.00
mm4__chr15 32.39 Mbp_chr15_32388319_R GTCTCGTGGGCTCGGGGAAGACAGGTACCAGCACC 60.0 0.00
mm4__chr15 30.15 Mbp_chr15_30150006_F TCGTCGGCAGCGTCGTGGCCAGGAAGACAGGTAC 60.0 0.00
mm4__chr15 30.15 Mbp_chr15_30150006_R GTCTCGTGGGCTCGGCAGAGAGAACAGGCCACCAG 60.0 0.00
mm4__chr20 53.35 Mbp_chr20_53345729_F TCGTCGGCAGCGTCTCCCCAAGAATGAGGCCAAG 59.6 0.00
mm4__chr20 53.35 Mbp_chr20_53345729_R GTCTCGTGGGCTCGGGTGGCAGGATGCTGAGAAGT 60.0 0.00
mm4__chr19 15.11 Mbp_chr19_15107824_F TCGTCGGCAGCGTCCCCACGACAGGAAATGGGG 60.3 0.00
mm4__chr19 15.11 Mbp_chr19_15107824_R GTCTCGTGGGCTCGGCTCCCATCTCCCATCCTGC 59.2 0.00
mm4__chr12 71.44 Mbp_chr12_71439682_F TCGTCGGCAGCGTCAAAGGAGGGAGGGGACAAGT 60.1 0.00
mm4__chr12 71.44 Mbp_chr12_71439682_R GTCTCGTGGGCTCGGCTGCTTGCACGCTCGTTTT 60.0 0.00
mm4__chr17 78.53 Mbp_chr17_78528591_F TCGTCGGCAGCGTCCGGTGCATAAGACAGGAGGG 60.1 0.00
mm4__chr17 78.53 Mbp_chr17_78528591_R GTCTCGTGGGCTCGGGAGTCTGGCCTGGAGTTTCC 60.0 0.00
mm4__chr11 117.30 Mbp_chr11_117299624_F TCGTCGGCAGCGTCTCCTGGCCTGGTTTCCATTC 59.9 0.00
mm4__chr11 117.30 Mbp_chr11_117299624_R GTCTCGTGGGCTCGGAAAGCACAGCATCTGGAGCT 59.9 0.00
mm4__chr6 13.59 Mbp_chr6_13594397_F TCGTCGGCAGCGTCGAGCCTAGTGGTGCAGCTTA 59.4 0.00
mm4__chr6 13.59 Mbp_chr6_13594397_R GTCTCGTGGGCTCGGCAGAGAGAGAAGGGAGGCCA 60.3 0.00
mm4__chr17 41.71 Mbp_chr17_41705870_F TCGTCGGCAGCGTCAGGGGCAGCAATCTATGTGC 60.4 0.00
mm4__chr17 41.71 Mbp_chr17_41705870_R GTCTCGTGGGCTCGGTCGCTCCTGCTGAATCTGTC 59.8 0.00
mm4__chr2 219.49 Mbp_chr2_219493659_F TCGTCGGCAGCGTCTGTCTATTGGGAGGGATGGC 58.8 0.00
mm4__chr2 219.49 Mbp_chr2_219493659_R GTCTCGTGGGCTCGGGACCCAAAGTGCTAGCTGGT 59.9 0.00
mm4__chr14 92.92 Mbp_chr14_92919710_F Primer3: not found at this Tm N.d. 0.00
mm4__chr14 92.92 Mbp_chr14_92919710_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 180.06 Mbp_chr4_180058822_F Primer3: not found at this Tm N.d. 0.00
mm4__chr4 180.06 Mbp_chr4_180058822_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 374.29 Kbp_chr7_374291_F TCGTCGGCAGCGTCAGGGCATGAATTGAGGGAGTC 59.7 0.00
mm4__chr7 374.29 Kbp_chr7_374291_R GTCTCGTGGGCTCGGATCTCTGAGGCCTCCCAACT 59.9 0.00
mm4__chr22 46.31 Mbp_chr22_46309493_F TCGTCGGCAGCGTCGATCTGGCTCTTGCTCGGG 60.2 0.00
mm4__chr22 46.31 Mbp_chr22_46309493_R GTCTCGTGGGCTCGGATTCCTCTGTACGTGCTGCC 60.1 0.00
mm4__chr9 101.34 Mbp_chr9_101342424_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 101.34 Mbp_chr9_101342424_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 175.13 Mbp_chr3_175132532_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 175.13 Mbp_chr3_175132532_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 46.69 Mbp_chr6_46687988_F TCGTCGGCAGCGTCGGGGAGTTGCCGAGAAAAGG 60.9 0.00
mm4__chr6 46.69 Mbp_chr6_46687988_R GTCTCGTGGGCTCGGCTCAGGGCCTCGAAATCCTC 59.8 0.00
mm4__chr7 132.46 Mbp_chr7_132456707_F TCGTCGGCAGCGTCTGGGGAGCCCTTAACAGACT 60.1 0.00
mm4__chr7 132.46 Mbp_chr7_132456707_R GTCTCGTGGGCTCGGCATGAATGGAGAGGGAGCCC 59.8 0.00
mm4__chrX 12.96 Mbp_chrX_12963762_F Primer3: not found at this Tm N.d. 0.00
mm4__chrX 12.96 Mbp_chrX_12963762_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 3.97 Mbp_chr2_3968991_F TCGTCGGCAGCGTCCCTCTCAGAACGTGCTCAGG 60.1 0.00
mm4__chr2 3.97 Mbp_chr2_3968991_R GTCTCGTGGGCTCGGAATGCTCTGGACAAGGCCAG 60.3 0.00
mm3__chr10 35.13 Mbp_chr10_35126952_F TCGTCGGCAGCGTCCGGAGTTCGAGCCTGGATTT 60.1 0.00
mm3__chr10 35.13 Mbp_chr10_35126952_R GTCTCGTGGGCTCGGGACCAAAAGTAGCGCTGCAG 59.8 0.00
mm4__chr1 24.90 Mbp_chr1_24901485_F TCGTCGGCAGCGTCACTCATCTTCTCTGGGGCCT 59.9 0.00
mm4__chr1 24.90 Mbp_chr1_24901485_R GTCTCGTGGGCTCGGTGCTGGCAGAGATGGTTTGA 59.5 0.00
mm4__chr10 43.13 Mbp_chr10_43128800_F TCGTCGGCAGCGTCCAGAATGAGAGACTGCGGGG 60.1 0.00
mm4__chr10 43.13 Mbp_chr10_43128800_R GTCTCGTGGGCTCGGGATCTCGAATGCTGAGCCCA 59.8 0.00
mm4__chr17 9.54 Mbp_chr17_9544013_F TCGTCGGCAGCGTCGAAGACTGGAACCCACAGGA 58.9 0.00
mm4__chr17 9.54 Mbp_chr17_9544013_R GTCTCGTGGGCTCGGTGAACCAGAGTGGGATTGCG 60.3 0.00
mm4__chr5 177.49 Mbp_chr5_177489074_F TCGTCGGCAGCGTCAGGGAGACAGAGAGCAGGAG 60.0 0.00
mm4__chr5 177.49 Mbp_chr5_177489074_R GTCTCGTGGGCTCGGGAAGCTGCACCCCTACCTTT 59.9 0.00
mm4__chr7 76.26 Mbp_chr7_76258281_F TCGTCGGCAGCGTCTATTTCAGCGACAGAGGCCG 60.1 0.00
mm4__chr7 76.26 Mbp_chr7_76258281_R GTCTCGTGGGCTCGGCCTGGCTCAATGATGTGGGT 60.0 0.00
mm3__chr17 50.15 Mbp_chr17_50152203_F TCGTCGGCAGCGTCTGCATCTCAGGCCTTTCTGC 60.6 0.00
mm3__chr17 50.15 Mbp_chr17_50152203_R GTCTCGTGGGCTCGGTGGAGTGGTAGGAGGGCTC 59.9 0.00
mm4__chr9 95.51 Mbp_chr9_95508247_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 95.51 Mbp_chr9_95508247_R Primer3: not found at this Tm N.d. 0.00
mm4__chr9 100.29 Mbp_chr9_100294209_F TCGTCGGCAGCGTCAAGTTATGTCGCCGCCATCA 60.1 0.00
mm4__chr9 100.29 Mbp_chr9_100294209_R GTCTCGTGGGCTCGGTCATACACCTGTGCCGCATT 60.0 0.00
mm4__chr7 150.34 Mbp_chr7_150341550_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 150.34 Mbp_chr7_150341550_R Primer3: not found at this Tm N.d. 0.00
mm4__chr18 35.13 Mbp_chr18_35128883_F TCGTCGGCAGCGTCCCACGTGGAAACCTTGAGGT 60.1 0.00
mm4__chr18 35.13 Mbp_chr18_35128883_R GTCTCGTGGGCTCGGAGAGGTCTGAGTTCCCTGGG 60.2 0.00
mm4__chr18 58.26 Mbp_chr18_58255790_F TCGTCGGCAGCGTCTTCATGGCATTCCACACCCT 59.5 0.00
mm4__chr18 58.26 Mbp_chr18_58255790_R GTCTCGTGGGCTCGGATGGACGAGCTCCGCTTAAG 59.8 0.00
mm4__chr9 129.04 Mbp_chr9_129036469_F TCGTCGGCAGCGTCGACCACTTTCGCGCTTCAAC 60.3 0.00
mm4__chr9 129.04 Mbp_chr9_129036469_R GTCTCGTGGGCTCGGTGAGCATCGCTGAGTCTCTC 59.2 0.00
mm4__chr3 24.40 Mbp_chr3_24399898_F TCGTCGGCAGCGTCCTTGGTCTCCAGTTGAATGTAAAT 57.1 0.00
mm4__chr3 24.40 Mbp_chr3_24399898_R GTCTCGTGGGCTCGGAAAGTTCTGGTGAGGTAACGT 57.1 0.00
mm4__chr8 133.57 Mbp_chr8_133571420_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 133.57 Mbp_chr8_133571420_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 121.93 Mbp_chr11_121929886_F TCGTCGGCAGCGTCCCAACGTCGTTCAACCACAG 59.6 0.00
mm4__chr11 121.93 Mbp_chr11_121929886_R GTCTCGTGGGCTCGGGCCCTTCAGAAACTACCTTGC 59.1 0.00
mm4__chr13 95.45 Mbp_chr13_95447884_F TCGTCGGCAGCGTCGTGGAGCCCGAGAAAGAGTG 60.3 0.00
mm4__chr13 95.45 Mbp_chr13_95447884_R GTCTCGTGGGCTCGGCACAGGAAGCAGAGGCAGAA 59.9 0.00
mm4__chr19 49.59 Mbp_chr19_49591159_F TCGTCGGCAGCGTCCATTTGCACGGGATTGTGGG 60.1 0.00
mm4__chr19 49.59 Mbp_chr19_49591159_R GTCTCGTGGGCTCGGCTCACGGTGCAGCCATCTTT 60.9 0.00
mm4__chr9 87.33 Mbp_chr9_87327116_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 87.33 Mbp_chr9_87327116_R Primer3: not found at this Tm N.d. 0.00
mm4__chr13 50.99 Mbp_chr13_50994222_F TCGTCGGCAGCGTCTAAACTGCAGAGCCTGGGTC 59.6 0.00
mm4__chr13 50.99 Mbp_chr13_50994222_R GTCTCGTGGGCTCGGCCTGACTCTGGAGGCTCACC 61.6 0.00
mm4__chrY 667.93 Kbp_chrY_667935_F Primer3: not found at this Tm N.d. 0.00
mm4__chrY 667.93 Kbp_chrY_667935_R Primer3: not found at this Tm N.d. 0.00
mm4__chr3 134.77 Mbp_chr3_134772488_F Primer3: not found at this Tm N.d. 0.00
mm4__chr3 134.77 Mbp_chr3_134772488_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 26.60 Mbp_chr11_26600471_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 26.60 Mbp_chr11_26600471_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 1.43 Mbp_chr1_1428110_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 1.43 Mbp_chr1_1428110_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 1.43 Mbp_chr1_1428032_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 1.43 Mbp_chr1_1428032_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 170.18 Mbp_chr6_170177294_F Primer3: not found at this Tm N.d. 0.00
mm4__chr6 170.18 Mbp_chr6_170177294_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 170.18 Mbp_chr6_170177257_F Primer3: not found at this Tm N.d. 0.00
mm4__chr6 170.18 Mbp_chr6_170177257_R Primer3: not found at this Tm N.d. 0.00
mm3__chr1 10.83 Mbp_chr1_10834503_F TCGTCGGCAGCGTCGCCAGTGAATCCAGGGAGAC 60.1 0.00
mm3__chr1 10.83 Mbp_chr1_10834503_R GTCTCGTGGGCTCGGAGCATGACTGATCAGAGGGG 58.8 0.00
mm4__chr22 31.38 Mbp_chr22_31377899_F Primer3: not found at this Tm N.d. 0.00
mm4__chr22 31.38 Mbp_chr22_31377899_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 9.80 Mbp_chr1_9798158_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 9.80 Mbp_chr1_9798158_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 2.44 Mbp_chr6_2436660_F TCGTCGGCAGCGTCCTCAGGTTAACAGGCCCCG 60.0 0.00
mm4__chr6 2.44 Mbp_chr6_2436660_R GTCTCGTGGGCTCGGTACCTGAGACCTCTCCTGCC 60.0 0.00
mm4__chr19 35.00 Mbp_chr19_34996516_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 35.00 Mbp_chr19_34996516_R Primer3: not found at this Tm N.d. 0.00
mm4__chr22 46.10 Mbp_chr22_46096423_F TCGTCGGCAGCGTCGTCACCATGGACCTGTTCCC 60.3 0.00
mm4__chr22 46.10 Mbp_chr22_46096423_R GTCTCGTGGGCTCGGATCCCACAGATGTGCTTGGC 60.6 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529558 CTCCCCTACCTGGTGCCTGGGGCGCCCCACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCT
TGCACATGCCGGAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATCCA
mm1__chr2 73.90 Mbp_chr2_73901390 AGGCTTCGCAGGAGATGATGCCCCCCGGGCTGTCTTCCCCTCCATTGTGGGCCGCCCTCGCCACCAGGTGCGTGCTCATC
TGGATA
mm2__chr22 15.72 Mbp_chr22_15721562 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC
C
ATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm2__chr2 131.26 Mbp_chr2_131263631 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC
C
ATCGTGGGGCGCCCCAGGCAGCAGGGCATGATGGGGGGCATGCATCAGAAAGAG
mm2__chr2 130.66 Mbp_chr2_130656825 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC
C
ATCGTGGGGTGCCCCAGGCAGCAGGGCATGATGGGGGGCATGCATCAGAAAGAG
mm2__chr2 130.46 Mbp_chr2_130463844 CTCTTTCTGATGCATGCCCCCCATCATGCCCTGCTGCCTGGGGCGCCCCACGATGGAAGGGAAGACAGCCCGGGGGGCAT
CGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm2__chr2 130.08 Mbp_chr2_130075272 CTCTTTCTGATGCATGCCCCCCATCATGCCCTGCTGCCTGGGGCGCCCCACGATGGAAGGGAAGACAGCCCGGGGGGCAT
CGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm2__chr14 19.40 Mbp_chr14_19402809 ATCATGTTCTGGTGCCTGGGGCACCCCACGATGGAAGGGAAGACAGCCCGGGGGGCATCGTCACCTGCAAAGCCGGCCTT
GCACATGCCAGAGCCGTTGTCAATGACGAGCAC
mm2__chr14 19.00 Mbp_chr14_18998943 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC
C
ATCGTGGGGTGCCCCAGGCACCAGAACATGAT
mm1__chr11 1.80 Mbp_chr11_1803996 CGCCACCCACATAGAAGTCCTTCTGACCCATGCCCACCGTCATGCCCTGGTGTCTGGGGCACCCCACGATGGAGGGGAAG
ACGGCCCAGGGGG
CATCGTCGTCGGTGAAGCTGGCCTTGCACATGCCAGAGGTGTT
mm4__chr11 73.27 Mbp_chr11_73271184 CATCTTACAGACGGGGAGGGCCCACCATGGGCCCAGGTAGAGCCGCCCGGGCTGCCTTCTCCTCATCAAGCCCAAGCTCA
AGGATGGAT
mm1__chr19 9.52 Mbp_chr19_9518225 AGTTAACAATGGCTCCGGCATGTGCAAGGTCAACTTCGTGGGCGACAATGCCCCCCGGGCCATCTTCCCCTCCATCGTGG
GGCATCTGAAGCACCAGGGCGTTATGGTGGGCATCGGTCAGAAGGACTCC
mm3__chr1 200.67 Mbp_chr1_200669800 CCAGGGGCCCAAGCTTCCGGGGCACGCTCACCCGCGCAGGACTGAGGCCGCGTCCGCTCCCGCTCCCGCTCGGGCCGTCT
CCCCCTTC
CAGGCTCCAGGCCAGGCTTCCGCCCGACAAGCCCTGACCCGCTCGTCAGGAC
mm4__chr9 135.68 Mbp_chr9_135677009 TGACACAGAAATGGGGTCCGGTCCGTGGGGCTCCCACTGTCATCTCGTCCCTGTGATGAGGAGAAGACAGCCTGGGAGGC
CGACGGCTGGTGTGGCCATGAGGCTTCTAACCCGACAGC
mm4__chr1 154.81 Mbp_chr1_154813398 CTAGGGCGAGGACAAGTGTGTGGTTTCCAAGGTAACACATAGCTGGGTGGATTCAGCCTCCCGGGCCGCCTCCTCTTCCT
GGGAGGCTTCATCTTAAGCCCGCAGGG
mm4__chr5 1.50 Mbp_chr5_1500505 CTCATCGGGTAGGTGTGCTTTATTTATATTCCGTGCTTAAAAGCCAGGAAGAGTTTAGGTCCACAAAGCAAGGGAAAGAC
GGCCCTGGAGG
GGTGGTGCCAGCAGGCAGCAGGCCAGGCCAACGTTGGCTGACATGCAGT
mm4__chr9 136.49 Mbp_chr9_136488355 TGGTGGACATGGACTTGGTGGCCTGGGGACTGTAGATAGGGAAGAGAAAAGGGCCCGGGTGGAGCTGGGAAGAGCTCCAG
TATTCGATGCCAGGC
mm2__chr5 57.48 Mbp_chr5_57482605 ATCTCCCACGTAGCAGTCCTTCTGGCCCATGCCTACCATAACGCCCTGGTGTCGAGGACGCCCTATCATGGAGGGGAACA
CAGCCCGGGGGG
CATCGTCACCACCAAAGCCTGCCTTGCACATCCCTGACCCAT
mm4__chr2 10.23 Mbp_chr2_10231797 ACTGCTGGACGAACACAGGCCTCCCTGAGAGCAGGCAGCGAGCAGCCCAGACCCAGCCTGGCTCAAAGGGGAGGAAGGCC
CGGGAGG
GATGGAGCCTGGAGCTCAAACCCTTTTGGAAACGCCTGTCAGAGG
mm4__chr2 131.63 Mbp_chr2_131626214 GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCAGGCTGTCTTCCCTTC
T
ATCGTGGGGCGCCCCAGGCACCAGGGCATGATGGAGGGCATGCATCAGAAGGAGT
mm4__chr21 42.33 Mbp_chr21_42332595 ACTTGACCTAACTCACCGGCCAGCCTCCTGGGCTGTCTCCCCCTCGAGGTTTCATGCCCAGAGTGACTGTTCACTCCTGG
AGCAGATCCTTGCCTTCACC
mm3__chr20 63.31 Mbp_chr20_63305078 ATAGGGTAGGAGCCTGTGGGAGTGGGCAGGGCCCTGGCCCCGGACTCTTCCTTCTTGGACCTGGCCCCTCGGGTCGTCAT
CCCCTCC
CTGGGCAGCCCTGTCTTCATAC
mm4__chr19 966.75 Kbp_chr19_966749 GGAGAAGCTGGAGTCTGCAGAGCCTCCGGAGAAGAAGATGGCCCTGGTGGCCGATGAGCAGCAACGGCTGATGCAACGTG
CACTCCAGCAGAACTTCCTGGCCATGGCGGCCCAGCTGCCCATGAGCATTCGGATC
mm4__chr9 119.37 Mbp_chr9_119369661 GGTTGCTTCCGAGGAGTGTTTTGTACATCTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCGCCT
CGAGGGAAGGGCGGCCCGGGTGGGCTGGGGCTGAGCCGTAGGGAGTTTCGTGGGTC
mm1__chr5 77.78 Mbp_chr5_77784962 CTCGTCATTGACAACGGCTCCGGCATGTGCAAGGCCAGCTTCACGGGCGACAATGCCGCCCGGGCAGTCTTCCCCTCCAT
CGTTGGGCACCCCAGGCACCAGGGCGTGATGGTGGGCATGGGTCAGAAGGATT
mm3__chr19 33.41 Mbp_chr19_33406855 CGCCATTATCTAGCCAGGGAAGGAGGGGAAGAGGGCACTGGTGGCTGCTGAGTGTGAGGGCTGGGACAGGTCTCTCCTCC
TAGCCAGGCTGGCCACCCACCCCCCATGCCCAGGAGCAACACAAAAG
mm4__chr14 104.89 Mbp_chr14_104886983 GGACTTCCACTCCCAGGACACCCACCTGATCTTGAAGGAGACGGAGACGGCCCTGGCGGCCCTGGAGGCCCGACTCCTCT
CTAATTCTGTGGATGCCGAGTGTG
mm4__chr1 22.01 Mbp_chr1_22009861 CTCGAGTCTCCGCCTTCATCGACTGGATTGAGGAGGTGAGGAGGGCAGGGCGGCCCGGAGGGCTTTAGGGTGGTGGCTCT
TCTGA
mm4__chr1 21.99 Mbp_chr1_21986687 GGTGTTCACTCGAGTCTCCGCCTTCATTGACTGGATTGAGGAGGTGAGGAGGGCAGGGCGGCCCGGAGGGCTTTAGGGTG
GTGGCTCTTCTGA
mm4__chr15 40.41 Mbp_chr15_40412694 TAAGGGAATTGACCGGGCTGGTGACAGTGTCTTGGAAGTAATGTCCCCTGGGGGAGGGAAATAAGGCCAGGGGGGTCGGG
AGGGCTCAGGGAACTGAGCATATCATTGAGAAAGAAGTGTCCCCATGAGAACTGGGGA
mm2__chr16 8.32 Mbp_chr16_8318062 GCCCCAAAGCATGAATGTGGAAAACCCACCCTGGCCGTCGTCCCCTCCTTCTTTGTCTTCTAGAGTCTCTGTGGCCATTT
GTTTCTTATTTGCCAGCCACATTGAACA
mm3__chr9 131.56 Mbp_chr9_131555000 GCCCAGCTCTACCCAGCCGCGCCCACGCCGCCCGCGCTCTTCTCGCCGCCCGCCGGGGCCGCCTTCCCCTTCCCCGTGGT
GAGTGGCCGCCGCCCTGGGCGCGCTCCCCTCCGTGCTGTCT
mm4__chr5 37.25 Mbp_chr5_37249104 TGGGTAGTGGGGATGTACCAGGGCCGGGTGCGGCGGGAGACGGCCCGGGAGGCGGAAGGTCGCGGACCTGCCCGACAGAA
GAGTA
mm4__chr7 1.53 Mbp_chr7_1529575 AGTGCAGAACCCGCAAGTGGGGGGGAGAGATGGCCCGGGAGGCTGCCCCCGCGCTCCACAGCAAGAGGCCACTGGGCTGC
TGCCTAAATCCCCAGCCACAT
mm4__chr15 41.86 Mbp_chr15_41856989 GGCGTCCCTGAAGAACTTCAAGAGGAGGCTCCGGGCCTCCAGGGCCGTCCTGCGCTCCTGCAGGGGCTCCCTCAGGCTCT
TGAACC
mm3__chr6 36.79 Mbp_chr6_36793238 CTCAAACAGAGGAGGCAGGGGAGGAAGGGAAGCCGGCCCTGGAGGGCTGGCAGGACAGCCCAGATCTCTCCAAATCAGGT
GAGCAGAGCGG
mm4__chr14 73.74 Mbp_chr14_73741441 CATAGCCCACCTCTTTCCCCAGTAAGATTCTGGTCCCTAGGAAGAGGGGAAAACAGCTCGGGCGGCTCTGAAGAGGAAAT
CTCAGGCCCTAGATGCTACAGGT
mm4__chr12 111.41 Mbp_chr12_111413614 GCAGCTGTTTTGAGGAAGGCTCCTGCCAGGGTTTGGCCCTGCAGTTGGGCTTCAAGACTCTGACATACAGGACTGTGCGG
AAGGTAGGACGGCCCAGGGGG
CGGGGGAGGTTGGTTCATGCTGGT
mm3__chr9 126.03 Mbp_chr9_126028189 ACAGAGCTCTCATCTCCCGATCTCTGCCCCCCAGGCCTTCCTCCCCTCCAAGAGTTGNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNAAGCAGCTGAACATTTAGTGAGCACTCACCCTACACTGAGCTCAGC
mm4__chr9 114.39 Mbp_chr9_114387826 GTCCCGGAGAGTCTGTGACTGGCCCGTCCAGAAGGAAAGCGGCCTCCCGGGCCCTCCTCCGTTCCAATCCCTTACCTCTC
TCGGGCTCCACCACCGTCCTGCCGACCCAGTTTCAGGGGACCTGCCTGTGAATTCT
mm4__chr3 197.51 Mbp_chr3_197510015 TCTGGGCTCTGGAGGGTTTCGGCCCTGCCTGCCTCCCCCCCTCCTCCTGGGGCAGCTGGGACAGGGGACCCCTGTTTGAA
GACAGCGGGGACAACGGCCCGGGAGGCAGCTGAATTGCCCATTGTGAGGCCCTTCTT
mm4__chr1 32.01 Mbp_chr1_32014784 GATGAGCGCCTCGACCGAGGTGTAGGAGGTGAAGACAGCCAGAGCGGGGAGGGACCGTGGGAGGAAGGGCCGAGGTGAGG
TGGGGTGAGGCTGGAATGCCCTGTCCAGTG
mm4__chr22 30.91 Mbp_chr22_30905726 AAGGCCAGAGGGAGTCCTGGACGCGGGGAGCTGGAGGGTGAGGCGACCCGGGAGGAGACACCGCCAGGCAGGGGAGGGCG
GCCGGGTAGGTGTGGTCCGGCTCA
mm4__chr19 49.36 Mbp_chr19_49357519 AAGGAGCTCGTGAACACCACGGACCCTCCCGGGACCCATCCCATCCAGGCCTCCTTCCCCTCCACCATGCTCTCTCTGTG
GCTACTCTGGGTCTAACACAACCACTAGAAAAACTATCCCCAAACAGGCACAGTGAGA
mm4__chr22 22.77 Mbp_chr22_22772713 GGAGGCCTATGGATAGAGCCATGAGTGAATCTGGTCCAGTTCACCTGGATCTGAGCCTCCCAGGCCGCCCTTCCCTCCAG
CCCCCTCCAGGAGTCTCTATAGAGCATACATCAGGCATACACATGGCCTGGAA
mm4__chr1 31.67 Mbp_chr1_31665449 TTGGGAGCATTACGTCTGCCTGGCCTGCTGGGCTGGGGCCCACACAAGAGGCTGGGCTCTCCAGGTCCTGGTTCCTGGAA
TCAGGCCTGGCCACCCAGGCCGTTCTCCCCTGCCTCTCCCCTCTTCTCCCCAGGACCAT
mm4__chr4 6.52 Mbp_chr4_6521612 GAGTCGTCTCAGGAGCCCTAACCCACGTCCCACCCCTCCAGGGCCCTCCTCTCCTCCCTCGAGGTGATGTGAGTTCATAC
GTAGGAGGCACTTACACAGGAGCTGGT
mm4__chr9 100.73 Mbp_chr9_100731316 GTCATTGACAACGGCTCAGGCATGTGCAAGGCCGGCTTTGCAGGTGATAATGTCCCCCTGGGCCATCTTCCCCCTCCATC
GTGGAGCTCCCTAGGCGCCAGGGTGTGATGGTGGGCATGGGTCAGAAGGACTT
mm4__chr5 141.75 Mbp_chr5_141748837 ATCCCTGAGGTTTGCCCATCCAGCCTTTTACCCACCTTTATCGCCTACTCCTTGGTCAGTAGCTCCAAACCTCCTGGGCC
GGCTTTCCCTGC
ATAAAAATCGTGCCTCTAACACTTGAAATGTGGGAAGTGAGAGCCAGA
mm4__chr11 111.22 Mbp_chr11_111221710 GCCAGGGGCTCTTAAGTATGTTTCTGCTTCCGTACAGTTTGTGGAGGGGAAGACAGCCCCCAGGGCACCCATGAAAGCAG
AGAGAGA
mm4__chr9 35.40 Mbp_chr9_35403655 GGGGTAAGACTTGAGGGGTGGGGGGAGAGGAGGGCCCGGGGGGATGGCAGACTGGGGTGAAATCAGCTGAAGACTCAACT
CTGGCCTCATAACTTCTATCTTGTGCTCACAGCCTCCTGGGAAATGAGG
mm4__chr5 169.40 Mbp_chr5_169404318 GGGAGGAGACTGGGAGCTTAAAGCCAGCTAAAAACAAATCTAGATGTCCGCCACCCAGGCCCTCTTCTTCTCCCCACTCT
CAGCCTGAACCTCCACCCCAGTCTTCATGTTTCTTCACAGGCAGCCA
mm4__chr17 61.41 Mbp_chr17_61408538 GGAGTCGCCCAAGTGAGGGGCTGCCCAGCTGCTCCCCTGCCACGCAGGCCACCCGGGCTGCCTGCCCCTGCTGCTTGGGA
CGTGTACAGCACAGAATGAGTATTTATTTAAATAAAGGAGAAAAGTGGGCTGCA
mm4__chr11 103.55 Mbp_chr11_103552304 GTCAGCTTGGGAGTGTGTGTGGCGTGAGAGGGGAAGCAGGCCCAGGAGGAAAGCTGGGAATGCTGACAGA
mm4__chr21 43.27 Mbp_chr21_43274379 AGTAGATGCTGGAAAGCCGGGTCTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGTTG
GAAATGCAAGGAGAAGGAAATGAAAGACTAGGGGAGTGGGTTTCCT
mm4__chr15 25.90 Mbp_chr15_25904820 GAGCTGTCAGTCTGGGTTCCAGGCCGATCCTCCCCGCAGTGGCTTTCTTCCTCTCTCGCCTGGAGGACATCTCATAGTTC
TTGCCCTCAGGGTCCCCCCAGGCCGCCTTATCCTCCTGGCTTTTCATCCCCTGCAA
mm4__chr9 135.67 Mbp_chr9_135666594 CATGGTCCCAGCCCAGTAAAAACCCCCAGGGCCCCCTTCCCTTCCCAGAGCCTGAGCCTCCCAGAGATACGCCTCTGAAG
GCACCTCTGCATTTCTGCACGA
mm2__chr3 175.98 Mbp_chr3_175978480 GAGTCCTTCTGACCCATGCCCACCATCACACCTTGGTGCCTGGGGCGCCCCACGATGGATGGGAAGACGGCCCCGGAGGC
ATCGTCGCCTGCGAAGCTGGTCTTGCACATACAGGAGCCTTTGC
mm4__chr20 32.43 Mbp_chr20_32434635 CACCACTGCCATAGAGAGGCGGCCACCACTGCCATCGGAGGGGGGGGTGGCCCGGGTGGAGGTGGCGGCGGGGCCACCGA
TGAGGGAGGTGGCAGAGGCAGCAGCAGTGGTGATGGTGGTGAGG
mm4__chr1 147.59 Mbp_chr1_147591706 TTCAGTCTCTGGCTTGGCTCCTTGTCTCTCCCTCCTGGCCATCCTCCCCTCCTGGAACTGCCCAAATCCTATTCTTCAAG
CCAGTTTGGCTTCTGATCCT
mm4__chr6 34.06 Mbp_chr6_34056709 GAGAAGTAGCGGTCGAAGCCTGGCAGGGAACCAGGACGTCAGGGCCTCACTGGCCTTCTTCCCCACCAGCCCCAGCCCTC
CCCGCCTCCCCCAGGATAAGAGATGGTCCAGGCGGA
mm4__chr19 48.33 Mbp_chr19_48333573 GGGTTTGGGAGAAGCCTAGGAGTGATCTTGAGGCTTGGAGGGGAGCGGAACAAGGCCCAGGAGGGTCGAGAGGGCCAGAA
GGCCCAGCTCTGGGGCTTTCGTCTGGGGACCAGTCAGGGGCTCTTG
mm4__chr3 127.03 Mbp_chr3_127028940 CCGTCCCACTACACTGCTGTGATGGAGGAGGGAAAGAGGGCCCCAGCGGCCCCACCCTCCAGCTCCCTCCTCCCCCAGAG
TGGCGCCAGGGCCGCATGGCGCGCATCATCCTGCAGGACGAGGACGTCACCACCAAGAT
mm4__chr11 477.98 Kbp_chr11_477980 GGGCGTTTGTATTTCCTGCCAATGATGGCGGCTTTTCATGAGCCACGGGGGCCGTCTTCCCCTGAGTACGTCGGGGCCTC
CTGCGTTTAGTCCGAAAACCTCACGTCTGCAC
mm4__chr7 100.01 Mbp_chr7_100009716 GCGGGTGATTCAACAAAGCCACTAAGAGGGGAAGGTGGCCAGGGAGGGGCAGGAGCACCACAGGAGGTCCACGTTCCATT
CA
mm4__chr19 39.19 Mbp_chr19_39187774 CTAGGCTTGGGCAAGTTGGAAGCGGTGCCTCGGCTGGGTCAGAGCCTCTTCCTCCTGCCGCCCAGCCGGGCCTTCCTGCC
CTCC
CTGCCCAGCAAGCAGAGCAACGTCAGGTC
mm4__chr16 173.64 Kbp_chr16_173640 CACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGCTGGGCCTCCCAACGGGCCC
TCCTCCCCTCC
TTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTGGGCAGCAG
mm4__chr1 7.05 Mbp_chr1_7050736 GGTGGGTGGAGGAGAGAGTATGGTGGGGAGGGGAAGGAGCCCCAGGGGGCCTTGGCCTCCACGAGTTTCT
mm4__chr21 6.08 Mbp_chr21_6075912 GAGTGTGGGGTGTGTGAGACGGCCAGGCGCACAGTGTGGGATGCCGTTGCTGGCGGGGGCGCGGGCCCATGGGGGACGGG
ACAGGTTCGGACGTGGGGAGGGCAAGGGGGCCCAGGAGGGTTCCCACTCTGCAGATGGC
mm4__chr7 101.55 Mbp_chr7_101550972 GGACTGGAGGCCTTTGAGTCTGCCCTTCCCGTGCCGGCCGGGCCATCCTCCTCTCCCTTCCTCTTCTGCAGACTCAGAGC
ACAGTG
mm4__chr15 82.34 Mbp_chr15_82336150 GAGCATCAGAGGCTGAACCCCTTGGTGAAGAGCGTCTGTCCCAGGACTTGGAAGGGAGGGAGGGGAATGTGGCCAGGGGG
G
CTGCCTGCAGGTGTCTGTCCTGCTGCTCCTCAAA
mm4__chr20 38.60 Mbp_chr20_38599569 TGTGCTGTAGTGTGGAGTGGGGGCTGCTTGGAGGGGCAGAAAGCCAGGGAGGAAATGCTGAGCACACAGCACACAGGCCC
TGGGGGGCTTTCTGGGTCTGAGGGAC
mm4__chr6 34.22 Mbp_chr6_34224039 CATTCCCGAGCTCCGCCCCCTCTGCCCGCCGTGGCCCCGCCCCTGCCGGGCCGCCCTCCCCTGCGCTGGGCGGCGGGCCG
GGTGGCAGGCGGGGGCGGGGCCCGGGCTGGCGCGGCGCCGGCCTGAGTCACACGCATGAG
mm2__chr13 111.92 Mbp_chr13_111915153 GGTGACTCAAGAGACCGTGGTTTAGCCCCCACCTGCCAGTCCCCTCCCAGCCGTCCAGCCCTGCTGCCTCTTCCCGGGCC
TTCTTCCCCTGC
CACCTCCACCTGCCTGCAGATCTCAG
mm4__chr17 4.51 Mbp_chr17_4513388 CCTGTCCTGTTGGTCGTCATCTGCCACCCGGTCTGTCTTCCCCTGTCCTGACTCCTGGAAAGAGAGTGGGCTTTGCATCC
mm4__chr14 37.20 Mbp_chr14_37197809 GCTCGTCGTCTTGGATTCGACAACCATAAGCATCCCGTTAGGGCAACCACCGCAGCAGACTAAGTTCGACTACTTTCTCT
CCAAACTCCTCCCGGGCCTTCTTCCCAAGCTCTCTCTTTCTCCTGGAGGCCC
mm4__chr6 46.21 Mbp_chr6_46205051 CGGCATGTGAAAAGCAGCTTTGCTGGGACAATGCCCTCCGAGCCATATTCCCCTCCATCATCGGGCACCCCCGGCACCAG
GGCGTGATGGTGGGCATGGGCCAGAAGGACTCCTACGTG
mm4__chr20 57.53 Mbp_chr20_57526424 GGAATGAGGAGGGGAGGAGAGCCCTGTAGGGGGCGCTGGGAGACCTTGGGGGGAATGGATAAGGAGGGGAAGAGGGCCCT
CAGGG
GGCGCTGGGAGACCTGGGGGGGAAATGGATAAGGAGGGGAGGATGGCC
mm4__chr18 79.61 Mbp_chr18_79609960 GGAAGATGGGACCCCTTGTGCCCCACCGTGACCACAGGACACCGTGCACTCTCAGGCGCGCCATCCTCCCTGACTGACCG
AGCTCCCCCAAGGCCGTCATCACCTCCTGGGTAAGGGCTTTGCCTGGGTGAGTGGAAAG
mm4__chr12 6.53 Mbp_chr12_6533828 ACCTTCCGTGCAGAAACCTCCCCCTCCCCCTCAAGCCGCCTCCCGAGCCTCCTTCCTCTCCAGGCCNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGATGCCAGGAGCCAGGAGATGGGG
mm4__chr1 52.55 Mbp_chr1_52552696 TACAACAGGTTTCGGTCCGGGACCGGGAGCCCCAGCCACAGTGTGGAGTTCGTCCCCAAAGCGCCCCAATGCCCTCCTCA
GGCCGCCCAGGCCGCCGTCCCCACCTCCCTTATCTCAGCCTTCCCCTGGG
mm4__chr11 76.19 Mbp_chr11_76190053 TCATGAGGAGTGAGGGGCAGCTGGGAGAGAGGGGCCTGGCAGCCTGAGGGCACGNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNGGAGATGCTAGGTAAGTGCGCTTGGA
mm1__chr6 88.28 Mbp_chr6_88276908 GAGTCCTTCTGACCCATGCCCACCATCACTCCCTGGTGCCTAGGGTGCCCCACAATGGAGGGGAAGACGGCCTGGGGAGC
CTTGCGCATGCTGGAGCAGTTGTCGACGACGACGAGCGCGGTGATAGCATCATCCA
mm4__chr17 42.80 Mbp_chr17_42796801 ACTGAGCAAGGAAGATCCCAACACTGAAGGGGTAGAAGGCCAGGGGGGCATGGAGAGTGCAGCTCCATTATAGTGAAGAG
CCAAACATATGTGAACTGTTTGCTGTGTGGAGGTGT
mm4__chr8 12.95 Mbp_chr8_12951417 GTCGTCGTCACCCAAGGTACTGCGCTGATGCGCTGCGGGCCGACCAGGTGCTCCCGCCGGGGCGTCTTCTCCTACGCAGG
AAGGGCCACGCCGAGAGAGGCAGGCAACAAGG
mm4__chr16 73.07 Mbp_chr16_73066590 CTTAGGCCAGTGCAGAGTCGCCGCACTGGGACCCAGGCGCCCCTCACAGGTGCACGTAGGGCATGGCCCAACGGCCCCTC
GGATGGGCGGGGAAACAGGCCCGGGCGGAGCAATTCGATCACCCCATTATGCCC
mm4__chr11 47.77 Mbp_chr11_47767266 CGGAGAGAGTTGCAGGATGGGCCTACGGCCGGGTACCGCCCGGGACTTCTTCCCCATCGCGAGCCCAAGCGCGAGCAGAG
AG
mm4__chr19 11.44 Mbp_chr19_11441326 GAGAGTCCCTGCAGCAGATGGCCGAGGTCACCCGCGAAGGGTTCCGTCTGAAGAAGATCCTTATTGAGGACTGGAAGAAG
GCACGGGAGG
AGAAGCAGGTAAGGAACCCG
mm4__chr15 24.96 Mbp_chr15_24956470 TTCGGAGTTTCAGCCGTACCCTCTTTAGGGTGCAGTGGTAAGGAGAGGAAGCCGGCCAAGGTGGGCGGCTGCCCCCTCCC
CAAAGTGCTGCTTGGGAAAGGA
mm4__chr1 2.87 Mbp_chr1_2868344 AGGCACCAGGGGTCAGTGGGAGGGTGGGCCGGCCCGGGGGGATGCTGTCGCCCGTCTGCAGTGCCAGCTCTTCTCCTGCA
CGCTCCCTCCCGGGACGGGCCATGGCCTTGCTGGGCCATACGTCTCTGTC
mm4__chr7 2.66 Mbp_chr7_2661760 CCTCCAGCATGAGAGCCAAATACCTCGCCACGAGCCAGCCTCGCCCTGACTCCAGCGGCAGCCACTAGACCGCGCCCGGC
AGCCACCCACCCCACGTGCCAACTTCCCCTCCCCGTGCCAGCACTGCCGCTTCCACCTG
mm4__chr9 89.42 Mbp_chr9_89422506 TGCTGGCTCTGTAACATGGGAAGTGGGACAGGCAGGCTGGGAGCCATGCAAGAGGAGACCTGAAGTCCAAGAGAGAGGCT
GGAGCCTACAGGCTGGTAGAGGAAGAGGGCCCGCGTGGGTCACGTCCAGGTCCTGTG
mm4__chr5 93.60 Mbp_chr5_93604272 GGGCTTAACAAAGGGCTCCGCGGGGGCACGGGGGGGCGCGGCCACGTGACAGGCCCGAGCGCGACGTCGCTGTCCAGCCG
CGGGGAGGGGCGGCCAGCCCGGGGGGCCGTGGGGCTTCTTGACATA
mm4__chr22 29.72 Mbp_chr22_29721106 GTAGCCCCTTCCCTGCAGCCCTGCGGGGACCCCCAGCCCGGCGCGCCGGGAAGGCGGCCCGGGAGGCGGTGGCGACAACA
AGTGGCCGGACTGGGCGGCTAAGTCGCGATAGCCAAGCCTGGAAGGG
mm4__chr19 41.97 Mbp_chr19_41969210 GGGCTTTGAAGGGACAGACAGAGGTGGGGAAGAAGGCCTGGCAGGGCAGAGAGCTCCAGCAGTGGGACACAAGGCTCCAG
ATGGGCTGGGCCAAGGGCATCCAGGAAGCCCCAGGTGCCCAGCAAAGGCCTTGATGCTG
mm4__chr5 78.51 Mbp_chr5_78510274 TGATGTTCCGGGCGAAGAAAGAGTCAGGAGTCCACGGAGTTAATCAAAACAAGAAAGTCGGTGGGGAAGGAGGCTCGGGC
GG
CCCGGGAAGGAAGTCGCAGCTGCAGTCATTCACTCCC
mm4__chr1 229.43 Mbp_chr1_229433010 GCAGCCTGACCTGGTGTCGGGGGCGGCCCACGATGGACGGGAACACGGCCCTAGGGGCGTCATCCCCGGCGAAGCCGGCT
TTCACCAGGCCGGAGCCATTGTCGCACACGAGGGCGGTGGTCTCGTCTTCGTC
mm4__chr4 189.51 Mbp_chr4_189513003 AGATGCCTCTAGAGCTCAGCCCTCCCAGGCCAGCTTCCCCTCACACAGAGAATGCAGGAAGGAGGAGGAAAGATGAGGTG
CCCGTGAGTGCGGAGGGGACACCAAGGGACAGCGGGCTGCCTAACCAAGGAGGGAG
mm4__chr17 28.26 Mbp_chr17_28256362 GGCCAGGGAAGAGGAAAAGGTGGCCTCTCCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNN
NNNNNNGAGCGGGCCCATCCTGCCCTCTAGGCTACAGTTCCAGCGC
mm4__chr16 169.38 Kbp_chr16_169383 TGTGAGTGCTGTGTTGACCTAAAAACACCATTAAGCTAGAGCATTGGTGGTCATGCCCCCTGCCTGCTGGGCCTCCCACC
AGGCCCTCCTCCCCTCC
CTGCCCCAGCACTTCCTGATCTT
mm4__chr10 131.70 Mbp_chr10_131695389 TCCAGGCTGGAAAGATGCTGGGAACAGTGGTGTGGAGGGGGAGAGGCCCCAGGAGGACTAGAATGTTCCAGGATCTGCTT
GGCT
mm4__chr19 14.57 Mbp_chr19_14565697 TGGATCGGTTTCGCCATCATGACGCAGTGTCTCCCAGGTGAGCCTGCCGCCCTCCCTCGGCGGGGCCCTGCCCCTCCGGG
CCGGCAGCCCCTCC
CTGACGCCCGTTCTTTCCT
mm4__chr19 19.06 Mbp_chr19_19056742 CTGAACCCAGACTCCTCCGTGCAGCCCCCGGGGCCCTCCTCGCCTCCTCACTCCCAGCATAGGATTCTTGGACACACCAG
GCTGGGACCCACAGGGGGCCTTTGCTCTCGCTGGCTCTTACTCTGG
mm4__chr11 64.76 Mbp_chr11_64759194 AACTAGGATGCTGCCAGTGCCTGGTCTCCCAGTTTCCTGGGTCCCCTCCTGGGCCGTCTGCCCGCCCCTCCCCCACTCCC
CACCGAGGCGTGTCAGGTTGCTGACATTTACCAGTGACCCCACCGGACACTTGATCTC
mm4__chr20 58.65 Mbp_chr20_58651205 GAACGTTGCGAGCAAGGCTTGCGAGCGTCGCAGGGGGGCACTCGCGAGGGACGGACAGGAAGAGGGCCAGGGGGGAACGC
CAGAACCCGGGACCACAGCACGGAAACGGTCCGGGGCTGAGCTGCAGTCAGATCC
mm4__chr10 71.74 Mbp_chr10_71742334 CACCAATGTGCATGGTGGTGTCTTACAGAAAGGGTGCCTCTCTGGCCCTCTTCCCCTACATTGTGTGGTGTGGCATGCTG
TCACAACACGTCAGGGT
mm4__chr2 19.80 Mbp_chr2_19799445 GCCGAAGAGTCCAAAACAGCATAATGGGTGCTATGATGGAAGGGAAGAAGGCTCCGGGGGAGGGACAAGGGAGAGGAGTT
AGGAATAGTAGCAATAAGGTGTGGCCAGGA
mm4__chr5 6.08 Mbp_chr5_6083772 TCACGGCTGTGTCAGGAAAAGCCCTAAATAGCATCCTGCCCACCCTGGCCTCCTGCCCCTCCCACAGGCTTGCTCACACC
TTCATCCCTGCCTGGCTCGGCACATTCCCTGTATCCGTCTACTCCCCTGCC
mm4__chr7 70.47 Mbp_chr7_70474285 CTCACAGAGGGCAACACGTAATTAGGTAATCTGACTCACAAGATGAAGTTTACTCGCTTTCATTGTGGTGCCCCCCAGGG
CAGCCTTCCCCACC
TGCCCTCTAGGCCTGTGTCCTTGTAGCACT
mm4__chr8 102.56 Mbp_chr8_102564519 ACAGTGTGGGAGTGAAGTGCCCTCCAATCTCAGAGAAAGTGCGGGGAGGTGAAGCAGGCCTGGGTGGGAGGCAGAGCCTG
GCTGTGGGCCCTGTCTGAGGCCTGCAGGAAAGGCACAGAGCAGGGCTTAGTCC
mm3__chr9 135.08 Mbp_chr9_135080244 GGTGGTCTGGATGAGCTGTCTTGTCCCCCTGCCAGGCCCTCTTCCCCTCCAGCTTCTCTGCCCCCTCTGTTTTGGACTTG
CTGGGAGGA
mm3__chr2 238.72 Mbp_chr2_238720744 CAGAAGAGGTGCACTGGGAGGTGGCGGCTCCTCCAACTCAGAAGAGATGGAATCCTCAAAGCATGAGGCAAGGCAGGAGG
GGCAGAGGGCCAGGGAGG
TGGCATAGCATTGCATGTTCTGCGTCTGTATCGACCAAACA
mm3__chr12 48.18 Mbp_chr12_48184029 CTCCGCTTCCCTCGGCTCCCGCTAGCCCCTCCCGGGACCTCTCCCCCTCCACCCCCTCCCCCACCCCGGAGGCCGGGCTG
GACGCGACCCAGAGCCTCCGCCACCCGCTTCTGCCACTCAATGGAGGACGGTCTGC
mm4__chr17 36.88 Mbp_chr17_36884620 CCGCTGCAGAAGGGATATGTGTGGCGTCTAAGTCAGCGGCAGGGCCCTCGGCTGGCTGCCACCAGGGCCCGCTCCCCCTC
C
AGTATGATGGGTGAGGGGTTGTCAG
mm3__chr3 12.07 Mbp_chr3_12070300 TCATTGACAGTGTCTCCGGCATGTACAAAGCTGGATTTGCTGGGGACGACGCCCCCTGAGCCGTGTTCCCCTCCATCCTT
AGGCGCCCCTGGCACCAGGGCACGATGGTGGGCATGGGTCAGAAGGACTC
mm4__chr11 67.38 Mbp_chr11_67379800 TCCCTGGGCTGGAAGATCTTTATCCTGCCCTGCTGAGTCATGCCGGCTCCTCCCGAGCTCTCCTCCCCTCCTACCCCGCT
GTCCTCCCCTCTGTCAGGCTTCTCCCAGCTTT
mm4__chr17 4.98 Mbp_chr17_4982788 CCCTGGACCTTCAGCTTCATGTGGTCCTCTCGGGTGGTCTTCCCATCCTTCCGCTTCTTCCAGAGGTAACCATCCTTCCG
ATATTTCACCTTCTTGCGATTGTAGAGGATGATGGAGCCATTCTGAGGCCTGGGAAG
mm4__chr7 44.18 Mbp_chr7_44176862 AATCACCCGATGCTTCCCTGCTCTGCCTGCCTCCAATCCAAAGATTTCCTCAACATCTGATCCTCCTGACCCACCCGGGC
CAACTTGCCCACC
TCTGAGAGGCTGCAGTTCTCATGGGGAT
mm4__chr1 29.93 Mbp_chr1_29926912 ATTAGATGCCAGCCACGAGGGTGTGCCACACAGAGCAGGCCACACTGGCAGTCTTCCCCTTCATCAGGTGCCCTTTGCCC
GAAGCCCTGGGGGAGGGGCAACACTGCTCCCTAGGTGG
mm4__chr11 72.68 Mbp_chr11_72678319 GGTCCCTAAGCACACTCTGGTTCTCTCCTCCTCGACTTTGTACAGTGTTTCTCCTCCCAGGCCGACTTCCCCCTCCTCTT
CCTTTTCTCTGCCTATCCCAAATCCTAGCCCACCATTGCTG
mm4__chr2 17.75 Mbp_chr2_17753339 GACCTCTCCCCGCTTTACAGTTATTCGCCACAGCCCGGGAGAGTCTGGGGACGGCCGCCTGGGAGGGGACGGCCGCCTGG
GAGG
GGACGGCCCGGAGCAGACGCCAGGTTT
mm4__chr6 167.98 Mbp_chr6_167977394 CTTCTCTCCCCTCCCACCCGGGACCTCTTCTCCCCTCCCACCCGGGGCCTCCTCTCCCCTCCCACCCGGGACCTCTTCTC
CCCC
CCATCCGGGGCCTGCTGTCCCCTCCCACCCGGGACCTCTTCTCCCCTCCCAT
mm4__chr1 48.78 Mbp_chr1_48776528 GGAAGGAGCAAGCAGTGGCTCCCCCCGGTCCCCTCCGCCCGGGCCCCCGGCCCCTCCCGGGGTCCCAGCCCCCGCCCGGG
TCCCACCGTCCCTCCCCGCCCGCTTGCTCACCTGCCAGCGCCAGGATCTGGGCCTTGTGG
mm4__chr10 114.04 Mbp_chr10_114043530 CAGGAGTGGGTGCGGGAGCGAGTGGGGGCTGAGGGAGGGGACGGGGCCCCGGGGGGAGCCAGGCGCGGAAGGGGGCGCGG
GGGAACAGGGACCAGGAACCAG
mm4__chr19 36.14 Mbp_chr19_36142599 TTTCCTCATCAAGCTGCCCAGCCCACTCTGACTTCCCCACCCAGGGTACCTGGGTTTGGGGAGCCGTCCTGGCCGGGTTC
CCCTCC
CCCTGCTCTGAGCTCTCCTCCCTTTGC
mm4__chr17 28.03 Mbp_chr17_28032578 GGAATTCTCCTGGGGCTCAGAGCCTGTGTTGTTAGGATGGGAAGAGGCCCAGTTGTAGGGGAAGAAGGACCAGGTGGTAC
GTGAACTTAGATTTGTCTGGCCTCACCCA
mm4__chr9 113.88 Mbp_chr9_113876825 TGCAATTTTCCCCTGACCCTCTCTGCGCTCGGGGTCCCCGATGACCCCCCGGGAGGTCTTTCCCCCCAGGTCCCCCATTT
TTGCAACAATCTTTTTTTTTAGGCCATATTTTCCTTTCTCAATTT
mm4__chr2 43.23 Mbp_chr2_43226811 CTTCCCGGCCTCCCATTGGCCGCCGCCAATTTTTTTCCTCCGGGGGAGGGTGCTGGAGGCCTCCGGGCGGGGCCGGCGGC
CCGGGCGG
CCGGTGTGGCGTGTAGCTCTC
mm4__chr9 87.31 Mbp_chr9_87314245 GAAGGACCAGCCAAAGACCAGGCTGAGTGCTGAGCCCAAGCATGAAAGGATGAGAGTGGAAGATGGCCAGGGTGGGCCGA
CATCAGAGAGCTTCGTGGAAGGATAACAGGGACCCCCATTGCAGAGTGCACCCTACATCA
mm4__chr9 130.32 Mbp_chr9_130318967 AGAAGGGGCTGTGGAAATGGCAGCTGAGTCCCGCCAGGCCCCCCAGGCCATGCTCCCCTCCACAGAAGTGGGCCTGGCTT
AGGCCAGGGGCTTAAGCATCAGAGGGGAGCTCAGGACACCTCCAAGA
mm4__chr15 85.25 Mbp_chr15_85253808 TTGAGTTGAGGAGCAGCAGGACAGGCACCTGCAGGCAGCCCCCCTGGCCACGTTCCCCTCCCTCCCTTCCAAGTCCTGGG
ACAGACGCTCTTCACCAA
mm4__chr6 33.44 Mbp_chr6_33438042 GTGGTACCCTGTAACCCTGCCAACAGGCAGTGGGGGATCTGGGGGCATGGGTTCGGGAGGGGGAGGGGGCTCGGGGGGTG
GCTCAGGGGGCAAGGGCAAAGGAGGTTGCCCGGCTGTGCGGCTGAAAGCACGTTACCAG
mm4__chr11 126.45 Mbp_chr11_126452845 AAGCCAGGTCTTTTGGGGAGGTGCCTCAGGACCCGACAGAGCCCGCAGCTCACATCTCGCCCAGCCGGTCTTTCTTCCCC
TCC
TGCACTGAAAAACCCTCTGAGAGGTCCCTGCCAAAA
mm4__chr17 77.85 Mbp_chr17_77846535 CCTCCCCAGTGACCTACCATGAGCCACCTGGGCCGACTGGCCCTCCTTCCCCACAGAGCCCCTGGCACCCAGGACTTCCT
GGCAGGTGCTGGCCCTGAAGTCCTGGGCTTCCTCT
mm4__chr12 122.15 Mbp_chr12_122147488 CCTCGGGAGGTGTGATAAGCCTGGGATGGAGGCATTGGGTGGGGAAGAGGCCCAGGGTGGGAGGGCGATTCTAGAGGTGT
GTCCAGAAAGTCGGTGCTC
mm4__chr19 4.66 Mbp_chr19_4664754 ATCAGTGCACTTCCTTGGGGAGCACTCCCACCCCAGCGACCCTGCCACCCCCCACCTGCACGTGCATGAGTCTCCCTGCT
GGTCTGTCTTCCCCTCC
AGGGGTGCAGCCCTCGTTCCCTGCCTTCCAAA
mm4__chr10 101.22 Mbp_chr10_101217493 TCATCCCTAAAAGCCGGCGCAGGCGAAAGCAGGCGCCGCCGGCGGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNGCCCGAATTGTCCGCTCCA
mm4__chr1 2.20 Mbp_chr1_2197690 GCACAACGTGTCACAGTTGTCCTCCTGGGGGCTCTTCCCCTCCAGTTTATCTGCTGCTCTGGATTAATCTCCCCTCCTCC
CATGTCACCACTG
mm4__chr10 8.07 Mbp_chr10_8069178 CCCTCTGGTGTACTTTGTGGGTTGAGGGTAGGAGGCTAAGACTGCCAGGGAGGAGGCCCCGGCTTCCTGCTCCTACCGGG
GAGCAGCAGGTGAAACTCT
mm4__chr17 41.82 Mbp_chr17_41822150 AAGAAAGCACTCACTGGCCTCCACCCGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNAGTTCTCCACCTTCATCAAGGCTCAAGTGAGTGAGGGCAAAGGAC
mm4__chr1 185.05 Mbp_chr1_185045466 TCGCTCGCTCGCTCCTTCCCGCCCTCCCCGCAGCGCCGGCCGAGCCGGCTTCCCCTCAGTCTCTCATGAATATTGAGCGG
CCCCTGTTGTATTTCCCGA
mm4__chr5 5.73 Mbp_chr5_5725807 ATCTGGGGCATGGAAATGGGCTGCAGCTCCAGGACCTTCTTGAATCCCACCATGTAGGGGAACACTGCCCTGGAGGTAGG
GATCTTGAAATTCTGCCTTTTCAAGAGCAGCA
mm3__chrX 103.71 Mbp_chrX_103707301 CCCACCCTATTCACAGAGCCTGGTGGATATCTTCTGACCATAGCACTTTATAGACAACCCAGTAGAAGATATTGAAGATG
AGGAAAGTGAAAGGGAAGACAGCCCGGGAGATGGTGTCAATTCTCTTGGCTCAGTCCACG
mm4__chr17 1.10 Mbp_chr17_1096680 TCCCTCTGGTCTCTGCTCAAGAAAGAGCCAAGCAGGGGCCCAGGAGGAGAGAGCTGGGGGAAGGTGGGGAACCCGCCCCG
GGAGG
AGAGAGCTGGGGAAGGGGGGGAACCTGCCCCGGGAGGAGAGAGCTGGGGAA
mm3__chr2 17.88 Mbp_chr2_17879296 CGTTCCTTCCCTTCCTCGAGAGGAGGCGCTTTCCTGGCGCTTGTAAATGCTTCCCGGGCCGTCTGCTCCGCCTTCTTTGT
CATTCCTCCCCTCCCCTTT
mm3__chr6 42.78 Mbp_chr6_42782779 GTGTGAGTGTGTTTTCCGCCCCCCGGAGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNN
NNNNNNNNNNNNNNNNNNNNNNGGCCTGGAGTGACGGCGGGCGAGGAAGAAAA
mm4__chr7 6.66 Mbp_chr7_6656105 GGTAGGAGGCACGGTAGTTGGTGGGTGGGAAGAGGGCCTGGGTGGTGGCGGTCAGTTAGCCTGGCTGNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGGGGTCCCAGGGAGGCATTGGGGTG
mm4__chr2 10.50 Mbp_chr2_10501046 ACAAACACACAGAGACGCCAGGGAGCACCTCCCCACCTTGGCTCCCTTCTCCTGCGGGTGCCTCCCGGTCCCTCCTCCCC
TGC
CCGAGGTCTGAAGGTGGGAATCTCCAACGCCCCTCTTTTACC
mm4__chr18 79.77 Mbp_chr18_79771319 CATCCTCCCCTCCACGCCGTCCTCCCCTCCACGGCGGCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTC
C
ACGCCATCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCAGCCTCCCCTCCATGACAT
mm4__chr18 79.77 Mbp_chr18_79771302 CATCCTCCCCTCCACGCCGTCCTCCCCTCCACGGCGGCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTC
CACGCCATCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCAGCCTCCCCTCCATGACAT
mm4__chr18 79.77 Mbp_chr18_79771132 GTCCTCCCCTCCATGCCAGCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTCC
ACGCCGTCCTCCCCTCCACAG
mm4__chr18 79.77 Mbp_chr18_79771115 GTCCTCCCCTCCATGCCAGCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTCC
ACGCCGTCCTCCCCTCCACAG
mm4__chr18 79.77 Mbp_chr18_79771098 GTCCTCCCCTCCATGCCAGCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTCCACGCCGTCCTCCCCTCC
ACGCCGTCCTCCCCTCCACAG
mm4__chr18 79.77 Mbp_chr18_79771064 TCGGAATCCAAAGGCGCAGGGCGGCCTCCCCTCCACGCCGTCCTCCCCTCCACACAGGGCTCCCCTCCACACCGTCCTCC
CCTCCACACAGGGCTCCCCTCCACGCCGTCCTCCCCTCCATGCCAGCCTCCCCTCCA
mm4__chr18 79.77 Mbp_chr18_79770996 CTGGGGTCGGAATCCAAAGGCGCAGGGCGGCCTCCCCTCCACGCCGTCCTCCCCTCCACACAGGGCTCCCCTCCACACCG
TCCTCCCCTCCACACAGG
mm4__chr7 129.99 Mbp_chr7_129990408 ATGAGAGGCCTTGAACTGGGCCCTGGAGGGGCAGAAGGCTTGGGAGGACTGTGATCACATCTCTGCTTAGGCACAGTGGG
CTGGGTTTGGACCAGATCATCCATGAAAAGCCACCATTCATCTCTGGC
mm4__chr8 12.44 Mbp_chr8_12436911 CTTAGGACGGGCCCACTTGGGGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGCTGCGCTCAGGAGGCG
GGCCCTGGGAGGCGGAGCTTAGGGA
mm4__chr10 3.54 Mbp_chr10_3536128 GCTGTCCTAGAAGCCTCACCTGATTGTGAACAAACCCCTGCCAGGCCATGTTCCCCTCCATTGCCAGGAGCCTGTTCATC
TTATTCCTGCTGCAGAAACCCA
mm4__chr1 156.59 Mbp_chr1_156591622 GTGTGGCGATCTGTAAACGCTCCTGAGGAGAGCTGGAATCCCAAGTGGAAGGGAAGCTGGACCGGGTGGGAACTGCTCCC
CAGCCCAAGGGCTGCTAGAACCCCAAGAC
mm3__chr2 27.12 Mbp_chr2_27123662 TTAAGGGAGCTGGAGATGCCCCGCACCAAGATGGCAGCTGGGGGAGGCCGCTTGTCTCGGGCTGCACAGCCAAGAACCGC
GGAGGGGAAGAAGGCAGGGGAGGGCGGGAGGCCATTCGCAGGTCACTCGGGTTAGAT
mm4__chr4 38.01 Mbp_chr4_38014537 CTCTCTCTCCTCAGGTGCCTGAGATCATCAGCTCCATCCGTCAGGCGGGGAAGATCGCCCGGCAGGAGGAGCTGCACTGC
CCGTCCGAGTTCGACGACACGTTTTCCAAGAAGTTCGAGGTGCTCTTCTGC
mm4__chr20 51.49 Mbp_chr20_51492633 CCACCAGCCAAGATAAGGGGACTGTGCGGGGCCCTCTGCGCGGCTTCAAAGGGCTGGCACCCGCCCGGGCCCGCTCCCAC
TCC
CACTGGCTAATGGGCTCCC
mm4__chr1 43.58 Mbp_chr1_43584522 GACAGCCCAGCCCAGAGTGGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
ACCCTATGGTCCCACCAAAAATAAGCACATGGACAAATATTTAGCGGGAAGGCAGACATG
mm2__chr10 24.95 Mbp_chr10_24952594 TTTGCATTTACCACGACGCCTCGCCCGTAGTCTGGCGCCTCCTCGCTGCTCCCGGCCATGTTTCTCCCGGGGAACGCGGG
AAGGGAAGACGGCGCGGGAAG
GAGCAGGGAGGGCGAAGG
mm4__chr11 6.41 Mbp_chr11_6410878 ACTCTCCAGGGGCTCAGGCAGCCCTGGGGGAGAAGATAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGCCCCTGCTCTTGAGTGCTG
mm4__chrX 37.35 Mbp_chrX_37350056 GTTTTGTAGGCAGTGGCGGGTGACAGTGGGAGGGGGTGCGTGCGAGGTCGGTCGCGGTGCGGCCCTGNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTGAAAGGAAGAGAAACAGTCTTCCTGCTT
mm4__chr2 264.54 Kbp_chr2_264540 GCCCCGTCCCCTATAGGTAACCTTTACCTCCCGCGGCTCCTTCCCCTCCAAGCGACCCGCAGCCCCGCCCCCTCCAGGTG
ACTCCCCCCCGCCAACCCCCGCCACCACACACACACACCC
mm4__chr9 89.50 Mbp_chr9_89497629 AGGGACTGGGGAGGCAACCGGGAAGGGGAGAAGGCCGGGGAGGGATAGCAGAGAGGTCCAGGGG
mm4__chr7 102.32 Mbp_chr7_102318012 CTGCAGGGGAGGGAGAATTGTGGAGGGGAAGACAGTCAGGAAGGCGATCCCAACAGCACCCAGCTGGATCAGAGGCATGA
TGGTACACCCAGGCGGTGTGCTTTCAAGGAAG
mm4__chr15 55.74 Mbp_chr15_55743497 CCGCGGCTAGTTGAGGATTGGGGAAGGGGAGGCGGCCGGGGAGGGCTAGTGTTGAGAGACAGGGAAAGGCCGGAGGCTTC
TCCCTTGATGGCCCCCGCCCACCCCCTCCTACACCCTCCTAC
mm3__chr2 85.44 Mbp_chr2_85437850 CATCCCACTCCGCACCCCCGGGCCTCCCGGCCCTTCTTGCCCTCCGACCCCGGGCTCTGGCAGGGCCGGGAGGCGCAGGA
ACCCCGCGGGGGATGGGGCCGGCGGACTGGCACTGAAGACACTGG
mm3__chr10 70.48 Mbp_chr10_70478534 GGTGTCTTCCAGAAGGAGCCGCACTAGCGACGAGGGAAGAGGAACTGGCTTCCCGGGCAGTCTCCCCCGCCCCAAACTTT
TCCTCCTCGCG
mm4__chr17 50.22 Mbp_chr17_50220935 CAGGTGCCTCATCCTTCCTCCCCGCTCTGCCTCCTGCGCCGTGGTCCCCTCCCACTGGGGAGGACCCGAGCTCCCCAGTC
ACCCCCAGGCCCTGCCGCTCAGGACTAGCTAACG
mm3__chr19 38.51 Mbp_chr19_38512045 TGGGGTGGATGTAGAGGGAGGCACTGTCCTCTGTCCTCTTAGCCATGGCATCCCCCCGGCCCATCTTCCTCTCCCAGGGA
CAGACGCCCCAGCTGTGGTCAACTGTCTTCACATCCTGGCC
mm4__chr8 142.83 Mbp_chr8_142834791 CCCGAAAGTTCCAGAAAGCGGGTGGGGAGGTCGGCGCGGGAGGCTCAGATGGCTGGAGGCGGCGGGCACGCACACTGCGG
GGCTCCGAGGGGCACAGGTCAGTTTC
mm4__chr7 131.59 Mbp_chr7_131592980 AGCAGTTTGGCCAAGGGTAGGAAACCTGTGGGAGCTGCTCTCACAGCCAGTTGCCCCTGGGGGTAGGAGGAGGGGAGGAG
GGCGCCGGAGG
GGAGGGCACGTCCTGCAGTTCAGCTGGTGATGGAGCT
mm3__chrX 153.69 Mbp_chrX_153694898 CACCATGGCTGAGGTAAGGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGAACATTCAACCCAGCCTGCT
mm4__chr22 47.97 Mbp_chr22_47965661 GCTGGCATGGTCTCTCTTGTGTCCCTTCTCCAGTGAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NN
NAGGGTTTGGCAATGGAAGGT
mm4__chr22 47.97 Mbp_chr22_47965643 GCTGGCATGGTCTCTCTTGTGTCCCTTCTCCAGTGAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNAGGGTTTGGCAATGGAAGGT
mm4__chr20 62.96 Mbp_chr20_62959057 ATGAACGAATCGGCAACCACCTGGAGGGGAGGACAGGCCTGGCGGGGGGTGGGGGCCCTCATCTTTGTTTACTGGAGGGT
CTTCGGGGTGAGGGAGGAGGGTCCCAAGGCTGAAGCTGTGAGGA
mm4__chr16_KI270728v1_random 1.14 Mbp_chr16_KI270728v1_random_1141254 AGCAGGCTGGGTTGAATGTTCATAGCAGGGGAGGGGAGGGCAGGCCGGGAGGGAGCCTTACCTCAGCCATGG
mm4__chr16 33.98 Mbp_chr16_33982366 CACCATGGCTGAGGTAAGGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGAACATTCAACCCAGCCTGCT
mm4__chr16 32.88 Mbp_chr16_32879145 AGCAGGCTGGGTTGAATGTTCATAGCAGGGGAGGGGAGGGCAGGCCGGGAGGGAGCCTTACCTCAGCCATGG
mm3__chr1 108.75 Mbp_chr1_108747063 CTTCTCGTTGGCGTCGGGCACCCCCTCGCCTCGGCCGCGCTCTGGCCGCGAAGCTCCGCCCCGGGTGAGCCGCCCGGCCC
GGCTTTCCCTCC
CGCGGAGCCCGTGCGTGCCCTCGGCCGCGCTCCCCACTCTTCTCCGCT
mm4__chr17 4.54 Mbp_chr17_4544987 CAGGCACACAACATGGGGACACCCGAGCCCTCCCCGGCCGCCCCCCCCTCCACCTCCCCCACTCACCAGCGCCTTCCCAG
CCTGCAGCACGGTCATCAGCTGTTCCCGGAA
mm4__chr15 90.96 Mbp_chr15_90957303 CATTGTGGGCCCAGAATGTGGTGCCCGAGGCCGAAGGGGAAGACGATCCGGCCGGTGAGGCCCAGGCTGGGAGGCTACCC
CTGCTGCCCTGCGCCCGTGCCTACGTGAGCCCGCGGGCGCCCTTCTACCGGCCTCTGGC
mm4__chr5 132.49 Mbp_chr5_132490001 ACAACGCTAAGACCAGGACGCTAACCCTTCCGGCCCGGTTTCCCCTCCTCTGGAGAAGTGGCCCAAGGAACA
mm4__chr16 85.69 Mbp_chr16_85692700 GGAACCAGAGTCAACAGACAGAAGGAAACCAAAGGGGAGGGGAAGAGGGCAGTGGTGGAGGTGGGGGTGCTGGACCTTCC
AGCATCATTTTCTGTTCTGTAAAACGCCTCCCCT
mm4__chr19 13.02 Mbp_chr19_13024952 ACGCTGCTTTTCTCCTTCTCTCTTTCCCCCTCATCCCGTCTTCCCCTCCTCCCGTCCTCCCTCGCCCCGCATGCTCCCGG
CTTGCCGCCTGCAGGATGAGTTCCACCCGTTCATCG
mm4__chr10 43.07 Mbp_chr10_43072967 TGGTGCTGAGCGAAAAGGATGGAGATTCCTCCAAGGGAAGGGGAGGAGAAGACGGGCAGAGGGGAAAGAAGAAAGGAGGG
CGAGGGAAGGAAATTCA
mm4__chr2 5.24 Mbp_chr2_5236303 GCTGTGGAAGACTCTCCTCGTTGCCTTCCCTGCAGTCTTCCCCTCCCTCCACCCTCGTTTCCCCACTGCCAAGTTCTGCT
TCCC
mm4__chr17 68.51 Mbp_chr17_68512843 GTTGGCTTTGGTGCGGGGCTCCCCAGCCGCCCCGCTCGCGCTCGCTGCCCCCACCCCACGGCTCCTGGGGCCCTGGGCCC
TCCGCGGCCCTCCCCGGCCGCCTTCGCCCCCGCGCGCCGCGGCCTCTTCGCTCACCCTC
mm4__chr3 128.18 Mbp_chr3_128175138 TCATCATCGGCATTCTCTCTTTTTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTAGTACTGCTGCAAACCC
mm4__chr8 1.97 Mbp_chr8_1974091 TAGGCGGGAGGGGAGAAAAGGGGAGGCCGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNGGACCGGGAGAGGAGGGG
mm4__chr7 50.34 Mbp_chr7_50336591 GTCAGATAGCTCGCCCTTGGGACAGGCGGTGGCGAGAGGCCAGGAGGCCTTTGGTGCAGGAGGGGAAGAAAGGCAGGGAG
G
GGCGTCTCAGAGGGCAGAGAAGGGCATCTCAG
mm4__chr6 132.70 Mbp_chr6_132698647 TGATCTTGGTGGCTCTGCAGAGACATCCACCCAGGAAGGCTTCCCCTCCCTGGGCCTCTCTGTTTGCTTT
mm4__chr5 111.07 Mbp_chr5_111073408 CTCTCGACTTGTGTTCCCCGCTCCTCCCTGACCTTCCTCCCCTCCCCTTTCACTCAATTCTCACCAACTCTTTCTCTCTC
TGGTGTTTTCTCCTTTTCTCGTAAACTTTGCCGCCTATGAGCA
mm4__chr6 43.94 Mbp_chr6_43939721 ATTGGGCACTGAATGGGTCCAGCACCCGACTCCTGGAGCTGGCAGCTCCCAGACCAGGAGGGGAAGTGGGCGCTGGGGGT
TGGCAGGCGGCCAACGCTAGGGGAGGCCTGAGCAGGGTTATGCCTG
mm4__chr11 78.03 Mbp_chr11_78030882 CAAGGCTCCCTCTGTGAGTCCTGCAGAGAAGGAGACCCCTGCCAACCCACAAGGACCCTCTTCCCCTCCCTCTGCTCCAT
ACCTGCTTGAGA
mm4__chr17 82.06 Mbp_chr17_82058578 CAAGCCAGATGCCTCTCCCTCACCCCCACCCACCCAGACTCTCTTCCCCTCCAGGCCTGGGCAGGGGCTTGGCTGGAAGC
AAGGGGCCGTCCTGAGGCATCCATGCCACCTTGAGCCACGTTGCAAAC
mm4__chr19 43.41 Mbp_chr19_43408444 CTATTCGAGGGGTGGCTGTGGGCCAGGAGGTAGCGGTGGCGGCGGCCTCGCCTGTGGCCATCTGAGCCGTGGGCCGGTAG
GCGGGGAGGAGAAGAGGGTCCGGCCGGTCACCGCCCTTGCACGCCTCCTCCTCTTAAA
mm4__chr1 6.94 Mbp_chr1_6943156 GACCCCATTAGCAGCGGTTAACACAGCCACTCCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNTGACCGTCCTCACCTGGCGTCCAGGACACCATGGTTCCTG
mm4__chr8 2.02 Mbp_chr8_2023960 CTCTGCAGGGAGGAGGAGGGAGGCTTGCGGCCTTCCCAGGGCCACGGGCCAGGAGGGGGAGGCGGCCGGGCTGGGTCAGA
GCCGAGGAGAGGGCGGCATCGGGAGCTGTTCTCAG
mm2__chr7 75.50 Mbp_chr7_75499258 TGACCTCTCCCTCTCTGTGTTCCTTTCTCTCCATCAGTATCTCCTGGCTATGGTCATAGCGTATTTCAGCCGGGCCGGCT
TCCCCTCC
TGGCAATACCAACGCATTCATTTCTTCCTGGCTCTGTGAGTGGTTT
mm2__chr7 102.66 Mbp_chr7_102657578 TGACCTCTCCCTCTCTGTGTTCCTTTCTCTCCATCAGTATCTCCTGGCTATGGTCATAGCGTATTTCAGCCGGGCCGGCT
TCCCCTCC
TGGCAATACCAACGCATTCATTTCTTCCTGGCTCTGTGAGTGGTTT
mm2__chr7 102.56 Mbp_chr7_102558494 TGACCTCTCCCTCTCTGTGTTCCTTTCTCTCCATCAGTATCTCCTGGCTATGGTCATAGCGTATTTCAGCCGGGCCGGCT
TCCCCTCC
TGGCAATACCAACGCATTCATTTCTTCCTGGCTCTGTGAGTGGTTT
mm4__chr20 22.26 Mbp_chr20_22263465 TCAGAGTCCTGTCTTTTGGCTGAAATCCATTTCTAAGTAAAACTCTCCATAGTGCCCTCCCTGCCCTTCTTCCCCACCCT
TCCTGTCCTAACCAGCTCTTCCCCAGAACAG
mm4__chr17 2.37 Mbp_chr17_2365370 GAGTGTCCCGAGCTTCATCCCGGGGGAGGAGAGGAAGACGCTCTGGGAGGCGGGGAGCGCAGCGTCACCCAGGAGGGCAG
CAGGCAGGGCCCACTGACCAGGCCAGGTTAACAC
mm4__chr16 27.32 Mbp_chr16_27318777 GAGTTGGAGGAAGTGGGTCCCGGGAGGGGAAGAGGAGCCGTGGGGCCTTCCAAGGGTTTGGTGGAATTGAGTTCCTGGTG
GGGAGGGGCTGTGTTCTGGGTCTTAGAGCCTCTCGCCTGCAAAGCAAAGTT
mm4__chr3 194.28 Mbp_chr3_194277433 CATCACCCACAGGCAGCCCGGGGAGCGCGGGCCTCCCGAGAGCCAGCCCAGGCCTGGATGGCCCACTGGGGAGGGCAAGG
AGGGGAAGAGGGGAAGGGAGG
CTGTGCCAAGGGCTGTGCCTGTGTTTCAGGGACAGCTGT
mm4__chr17 63.70 Mbp_chr17_63700367 CCGCCTTTGTCTGTCCCCCGCCCCGGCTCCGCGCTCCCGGCTCCGCAGCGCCCCGCGGGCCTCGCGCTCGCAGGCCTCGG
GTACCGGCCGCGCCGTCTGCCCATCCCGACGGCGGCCCGGCGTGGGGAAGCAACGGTGG
mm4__chr6 39.19 Mbp_chr6_39189897 GTGGGCCAAGAGCAATTTGGCCTCACCCTGCCTGGCCCAAGGGGGCTGGTGGGAGGGGAAGGCGGGCAGTGAGGTCCTTG
TTCAGGGCACAGGGAAGGAGAAGAC
mm4__chr19 782.06 Kbp_chr19_782056 ACAGACCAGGCAGCTGAATCCCCGCTCTACAGACAACCCCATCAAGGCTGGGGCAGTGAGACGCTCTGAAGGCCACAGGC
CCCCAGGATCCCACCCCGTCCGTCTCTCCCTCCCTGCTGTCCCTCTGTGAGCCACAC
mm4__chr20 61.84 Mbp_chr20_61835986 TGCCAGAAGGACCCTGACTACCCACAGGCTGGAGGGGAAGGCCGGCCGGTTGGGTGATCCCAGGGCTCTGGGTCTTGACC
GGGCTCTGGCATTGCAAGGAAGGGCTCCATCTGTGTTTCCA
mm4__chr5 132.67 Mbp_chr5_132667977 ATGACTCAAGAGCCCACAGCTCTCCCTGCCACCATCCCACCCGATTCGCGCCAGCTGCCTCTGTCACGGGCGCCTGCAAT
GCTCTGGCTGGGTCCCCTCGGCCCGTGCTCCCCTCCTGGGAGCCCTCTCTTCAGTG
mm3__chr5 149.42 Mbp_chr5_149424539 ACTCAAGGGAAGAGCTGCTGGAGCCAGGAGAAGCACCTCCCTCCCGGCCCCTCTGCCCCTCCTCATAGCCCAGCTGCACT
GACTCCTCCTCCAGGA
mm4__chr2 176.10 Mbp_chr2_176099645 TCGCTCAACCTGATTTGGCTCTGCTGGCAGCCTCGTCCTTCGCCATCGAACATTGCGGGTGTTATCATAATACTCTGAAG
GGGGGGAAAACGGGTCGGGGGG
ATGTAGGCGGTGCTGAAATGA
mm4__chr3 195.76 Mbp_chr3_195763320 ATTTACTCCGGGACAGCTGCGTGGATGGGCTGTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNT
CTCCTCGGCCTCAGTATGT
mm4__chr1 9.38 Mbp_chr1_9383327 GACCTTGTGCAGAGCAGATGGCGCGGGTGTTCAAGGAGGGGAAGATGGGCCCTGGGGAGGGAGGAAAGCCACCTCCAGGC
TCGGGGCTGCAGACCAGAGGGGTGGGAACTGGCAAGGGGAAGGCTGACAGCAAGGAA
mm4__chr11 44.67 Mbp_chr11_44668950 TTGAACAAAGCAAGCTCCCTTAGCCCACAAGCCACCCCAGCCATCTTCCTCTCCTGCCTCTCTCACTGCTGATTAACCTT
CCCAGGCGACATG
mm4__chr17 17.79 Mbp_chr17_17792869 CTGAGGCAAAAGGAAGTGGCCCGTCTGAATCGCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNTTTTCTTTTCACAGATAACCAGCCCGAGTCATGC
mm4__chr11 64.83 Mbp_chr11_64832786 AAAGGCAAGCATGGGAGGGCTGCAGGGACCCTCAGCCCCCCATGCCCATCTTCCCCTCCCTCGGCCCCCACTCACCGACA
GAAAGCCCTCAT
mm4__chr1 17.67 Mbp_chr1_17672194 ACACCAACAGCCCCTTGATTTCCCCTGCCCACCCCCCGCCCCAGCCGCCTTCCCCACCCTTAATTTTCATGTTTAGTGCC
TAAACTTAGTTTGCATGTATTAGCTGTTGTAAATCTATCATACGAGTGTGATGCGGCAT
mm4__chr16 57.52 Mbp_chr16_57517076 CCCTCTCTTGTTGTCCAGGGAATGTCAACAGCTTCCCCCCAGCCTGTCTTCCCATCCTCTGCCCACACAGCCTGCTCACA
CTCCCCTCCTGCTTT
mm4__chr16 88.24 Mbp_chr16_88240818 AGCCCCTTGTGATGGAAAAGTCCTTCCCGGCCCACTTCCCCTCCCCAGCTGACGCCAGGGACCCTCAGTGTGCCCCAGCC
TGCACCGCCAGCCCTCTCTCAGTGGGGGTAAGAGACACACAAGCCACCTG
mm4__chr16 50.63 Mbp_chr16_50633524 AGAGGGGCAAGAAACAAGCCCCCTCTGGGACCCGCCATCCCTGCGGTGTCCCCCTCCGCCCACCTGGCTGCCAGCCCGGC
CCTCCTCCCCTCC
CTAGCCCCCCTCGGGCACAAGAAGCACAAGCACCG
mm4__chr22_KI270734v1_random 135.21 Kbp_chr22_KI270734v1_random_135212 TGAAGCCCTCAGACTGCAGCCTCCCTCCCGCCTGCCTCCTCCAGCTGCCCTGGGTCTGGGGCTGCAGCTTTGGCCCCACC
TGGCCAGTCTTCCCCACC
CCCACTCTCTTTCAGTGCCCCGCACCTTCTGGAATCCCCTGG
mm4__chr22 20.32 Mbp_chr22_20316446 GTCTCCAGGGGACTCCAGAAGGTGCGGGGCACTGAAAGAGTGGGGTGGGGAAGACTGGCCAGGTGGGGCCAAAGCTGCAG
CCCCAGACCCAGGGCAGCTGGAGGAGGCAGGCGGGAGGGAGGCTGCAGTCTGAGGGCTTC
mm4__chr22 18.91 Mbp_chr22_18909941 TGAAGCCCTCAGACTGCAGCCTCCCTCCCGCCTGCCCCCTCCAGCTGCCCTGGGTCTGGGGCTGCAGCTTTGGCCCCACC
TGGCCAGTCTTCCCCACC
CCCACTCTCTTTCAGTGCCCCGCACCTTCTGGAATCCCCTGG
mm4__chr17 17.28 Mbp_chr17_17280911 AAACTGTCAAGCAAAGCGCCCGGTGGAAGGGGCCCAGGCCGGGGGGAGGGGGCTCCCGGCGGCCCGAGGGAGGGGAGGCC
GGCCGGCGAGG
ACAGCGGAGCGCGAGGGGGCTGCCTACACTCGG
mm4__chr15 32.39 Mbp_chr15_32388319 GACAGAGAGAACAGGCCACCAGGTGCAGACAGGGGAGGGAGGCAAGGTGACGGAAGGGAAGACGCCTGGGGTGGGTGGAA
GTCAGTGCCCTTAGGTGCTGGTACCTGTCTTCC
mm4__chr15 30.15 Mbp_chr15_30150006 GTGGCCAGGAAGACAGGTACCAGCACCTAAGGGCACTGACTTCCACCCACCCCAGGCGTCTTCCCTTCCGTCACCTTGCC
TCCCTCCCCTGTCTGCACCTGGTGGCCTGTTCTCTCTG
mm4__chr20 53.35 Mbp_chr20_53345729 TCCCCAAGAATGAGGCCAAGAGCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNACTTCTCAGCATCCTGCCAC
mm4__chr19 15.11 Mbp_chr19_15107824 CCCACGACAGGAAATGGGGCCGCGACTGAGGGCCTGGAGGGTGGAGGGGAGGAGGGGCTGGGAGGCCGGGACACTGGGGC
CCAGTCGGGCTGGGCAAGGGGCATCGGGAGCCCTGGAGGCGCAGGATGGGAGATGGGAG
mm4__chr12 71.44 Mbp_chr12_71439682 AAAGGAGGGAGGGGACAAGTGGAGGGAAAGTGGGGCCGGGCGGGGGGTGCCTGGGAAGCCAGGCTGCGCTGACGTCACTG
GGCGCGCAATTCGGGCTGGAGCGCTTTAAAAAACGAGCGTGCAAGCAG
mm4__chr17 78.53 Mbp_chr17_78528591 CGGTGCATAAGACAGGAGGGTGGGCCCTGTCTGTGGCTGGACACAGAGAAGGGGAGAGGGCGGGAGGAGGGGTGGACGGG
CTGGGAGG
AGTTGGAAACTCCAGGCCAGACTC
mm4__chr11 117.30 Mbp_chr11_117299624 TCCTGGCCTGGTTTCCATTCCCCTCCCTGCCCCTCTTCCCCTACCTCTACCCGTGGTCTCTCTCCCCGGTCTCTGGGCAG
CCACCAGCTCCAGATGCTGTGCTTT
mm4__chr6 13.59 Mbp_chr6_13594397 GAGCCTAGTGGTGCAGCTTATGTCATCTTTCCTCCCGGCCCCTGTCCCCCTCCTTCTTTCAGCTTTGCTCACTCTTTGAG
GTGGCCTCCCTTCTCTCTCTG
mm4__chr17 41.71 Mbp_chr17_41705870 AGGGGCAGCAATCTATGTGCATCACACAGAACTGAGATGACAAACCCTGGGAGGGGTAGGCGGGCTGGGAGGAGCCAGAC
AGTGGAGGAGCTGGAGGTCACAGTGAAGACAGATTCAGCAGGAGCGA
mm4__chr2 219.49 Mbp_chr2_219493659 TGTCTATTGGGAGGGATGGCTGGAGGGGAAGATGGCTGGTGAGGGGTGAGTCCGGGACAGGGGCATTTAGCCCTCTCTGG
GTATTCCCCAACACACACATTCAGGAATATACCAGCTAGCACTTTGGGTC
mm4__chr7 374.29 Kbp_chr7_374291 AGGGCATGAATTGAGGGAGTCGGTGAATGAATGAGCTGAGCCAGCCGTGTTCCTCCCCAGCCGTGTTCCTCTCCCTCTTC
CAGGGAACAGCGGGGCCAGCGGGGGGGGACCCCCTTTCCAGTTGGGAGGCCTCAGAGAT
mm4__chr22 46.31 Mbp_chr22_46309493 GATCTGGCTCTTGCTCGGGAGAGGCCACAGAGAGGAAGACGGGGCGGGTGGGAGCCCCGGGGCCCACCCTGCAGCCCGTT
CCCAGCTCTGCCCCCTCCCCTGGGCAGCACGTACAGAGGAAT
mm4__chr6 46.69 Mbp_chr6_46687988 GGGGAGTTGCCGAGAAAAGGCCTCGCCGGCATTCTTCCCCTCCACTGGGTCCTTTGAACCTAGTTTGGCTGGGACTCGCC
TTCAGGCGGCGCGGAGGATTTCGAGGCCCTGAG
mm4__chr7 132.46 Mbp_chr7_132456707 TGGGGAGCCCTTAACAGACTGAGCCCAGGATGGGCCAGGAAGAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNN
AGTTGACGAATCAATCAGAAAGGCTGAGGGCAGGGCTCCCTCTCCATTCATG
mm4__chr2 3.97 Mbp_chr2_3968991 CCTCTCAGAACGTGCTCAGGCTGGGGGGTTTCAGCAAGTGTCCAAGCCCCCCCAGCCCCGTCTTTCCCTCCCAGGCACGT
GCTGTCCTGCCTCCTGCCATCTGGGACTGGCCTTGTCCAGAGCATT
mm3__chr10 35.13 Mbp_chr10_35126952 CGGAGTTCGAGCCTGGATTTTTTTCCTCGGGGCCTCCCCCGGGAGGCCGTCCCGGCGTGGGGGAGGGGAGGACGGGGCGG
GAGG
ACGCGGTTCGGTCGGCTGCAGCGCTACTTTTGGTC
mm4__chr1 24.90 Mbp_chr1_24901485 ACTCATCTTCTCTGGGGCCTGGGACCCGTGCGGCTGGGGGAAAGCCAACAGTTAGGAACGGAGGGGAAGCTGGGCTGGGG
GG
ACTGGCTTGGATGTGTTCTCAAACCATCTCTGCCAGCA
mm4__chr10 43.13 Mbp_chr10_43128800 CAGAATGAGAGACTGCGGGGGGGGCCTGGGGGTAGTGTCAATGCCCCTCCAGGGCTGGAGGGGAAGAGGGGCCCCGAGGA
TGGGCCTGGGCTCAGCATTCGAGATC
mm4__chr17 9.54 Mbp_chr17_9544013 GAAGACTGGAACCCACAGGAAAATCAGTTTCATTTTCTTCAGCTTGTTCTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNACCTCCCTGCCCCTCTGCCCCTCCACCGCAATCCCACTCTGGTTCA
mm4__chr5 177.49 Mbp_chr5_177489074 AGGGAGACAGAGAGCAGGAGGGAGGGGCAGACGCGCTGGGAGGGGGCAGGCCCCTCTTTGCCCAGAGAGCAGCAAAGGTA
GGGGTGCAGCTTC
mm4__chr7 76.26 Mbp_chr7_76258281 TATTTCAGCGACAGAGGCCGGGCTGGAGGAAAGGAGGGGAAGCGGGGCAGGGAGGCACTCAGCCAGCGGGAGGGGGCTCA
GGGACTAGGACACCCAGGTGAGGAAAGACCCACATCATTGAGCCAGG
mm3__chr17 50.15 Mbp_chr17_50152203 TGCATCTCAGGCCTTTCTGCGGCCTCTGCCTCCTCCCCCGCCCTCTTCCCCTCCATCCTCTTCCTCCTTTTCCTAATCTC
CCACTCTCTCCCCCCTGGCCTGGAAGGGCTGGCAAAGGGAGCCCTCCTACCACTCCA
mm4__chr9 100.29 Mbp_chr9_100294209 AAGTTATGTCGCCGCCATCACTGGGGAGAGGAAGACGGATGGGGAGGGAGACAAGGCAAGGAAATAATGCGGCACAGGTG
TATGA
mm4__chr18 35.13 Mbp_chr18_35128883 CCACGTGGAAACCTTGAGGTTCTCTCCCGCTTATAGCCTGAACCGCCCAGCCCCACTTCCCCTCCTTGGGGCTGGCTGTA
GGCTCCCGCTAGCTCTCCAGATCACACACAGAGCCCCATCCCCAGGGAACTCAGACCTCT
mm4__chr18 58.26 Mbp_chr18_58255790 TTCATGGCATTCCACACCCTACCGCGGAGAAGCAGCCCGCACCCCTTCGCCCAGAACGGAGGGGAGGACGGCGGCGGAGG
CCTGCAGGGAGGCGTGGGTGCGCTTAAGCGGAGCTCGTCCAT
mm4__chr9 129.04 Mbp_chr9_129036469 GACCACTTTCGCGCTTCAACCGCACTAGGAGGAGGGGAAGAGGGCGGGAGAGGCGGAATATAAGCTTGGTCACATGACCC
GCCTAATTGGCCAGAGGCTGCGGCGGCCCTGAGAGACTCAGCGATGCTCA
mm4__chr3 24.40 Mbp_chr3_24399898 CTTGGTCTCCAGTTGAATGTAAATAATCAGAAAATATTAACTACCAAAAAAGGAGGGGAAGAAGGTAGGGGAGGACGTTA
CCTCACCAGAACTTT
mm4__chr11 121.93 Mbp_chr11_121929886 CCAACGTCGTTCAACCACAGCCAAGAGGGACCTGGGAAAGTCTTCTTAAGAAGGAGGGGAAGGCGGGGTGGGGGGCTTGA
GGGAGGGTGGGAGCGGGGGATGGGAGCAAAAAGAAAGGCAAGGTAGTTTCTGAAGGGC
mm4__chr13 95.45 Mbp_chr13_95447884 GTGGAGCCCGAGAAAGAGTGTCCTCCCCAGGAGTCTTCCCCTCCCACCTGCAGCATCAAAGCTGCCCTGCGGGGGTGCAG
CTATGCCGCCTCTCCCTTGCCAGGCTCTTTCTGCCTCTGCTTCCTGTG
mm4__chr19 49.59 Mbp_chr19_49591159 CATTTGCACGGGATTGTGGGAGGAAGCGGAGCGCAGCCAGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNTTGTGCAAAGATGGCTGCACCGTGAG
mm4__chr13 50.99 Mbp_chr13_50994222 TAAACTGCAGAGCCTGGGTCTCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNN
NNNNNNNNNNNNNNNTCCCACCACCCTTTAAAACAAGGTGAGCCTCCAGAGTCAGG
mm3__chr1 10.83 Mbp_chr1_10834503 GCCAGTGAATCCAGGGAGACTGAGGCTAGAGCAGGGAAGGAGGGGAACACGGGCGGGGGGGGGTGGCACTGCATGGTATA
GAGCATTTCCACCCCCTCTGATCAGTCATGCT
mm4__chr6 2.44 Mbp_chr6_2436660 CTCAGGTTAACAGGCCCCGGGCAAAAAGTCTCCATTCCCTGCCTCCCCGCCCGGCTTGCCCTCCCCTGGGGCTGGAACCG
TGGGCTTTGGCAGGAGAGGTCTCAGGTA
mm4__chr22 46.10 Mbp_chr22_46096423 GTCACCATGGACCTGTTCCCATTTTCAGAGATGGGCTGATTATGGCACAGCCTCTGTGGGATGGGAAGAGGGTCGGGGTG
G
GGGTGCTGGCTGTAGGCTTTGCCCAGTGTGTGGCCAAGCACATCTGTGGGAT

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

Version 4.7 - Documentation  - Contact us - Downloads/local installation - Paper - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.