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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529602_F | TCGTCGGCAGCGTCCACGATGGAGGGGAAGACG | 59.8 | 1.00 |
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529602_R | GTCTCGTGGGCTCGGGCTATTCTCGCAGCTCACCA | 60.1 | 1.00 |
| mm0__chr5 77.78 Mbp_chr5_77784918_F | TCGTCGGCAGCGTCATATCACCGCGCTCGTCATT | 59.9 | 1.00 |
| mm0__chr5 77.78 Mbp_chr5_77784918_R | GTCTCGTGGGCTCGGAACGATGGAGGGGAAGACTG | 59.0 | 1.00 |
| mm1__chr22 15.72 Mbp_chr22_15721518_F | TCGTCGGCAGCGTCATACTGCCGTGCTCGTCATT | 59.8 | 0.94 |
| mm1__chr22 15.72 Mbp_chr22_15721518_R | GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG | 60.0 | 0.94 |
| mm1__chr2 131.63 Mbp_chr2_131626170_F | TCGTCGGCAGCGTCATACCGCTGTGCTCGTCATT | 59.8 | 0.94 |
| mm1__chr2 131.63 Mbp_chr2_131626170_R | GTCTCGTGGGCTCGGCTTCTGATGCATGCCCTCCA | 60.1 | 0.94 |
| mm1__chr2 131.26 Mbp_chr2_131263587_F | TCGTCGGCAGCGTCTGGATGATGATACCGCCGTG | 59.9 | 0.94 |
| mm1__chr2 131.26 Mbp_chr2_131263587_R | GTCTCGTGGGCTCGGGATGGAAGGGAAGACAGCCC | 60.1 | 0.94 |
| mm1__chr2 130.66 Mbp_chr2_130656781_F | TCGTCGGCAGCGTCATACCGCTGTGCTCGTCATT | 59.8 | 0.94 |
| mm1__chr2 130.66 Mbp_chr2_130656781_R | GTCTCGTGGGCTCGGGATGGAAGGGAAGACAGCCC | 60.1 | 0.94 |
| mm1__chr2 130.46 Mbp_chr2_130463888_F | TCGTCGGCAGCGTCGATGGAAGGGAAGACAGCCC | 60.1 | 0.94 |
| mm1__chr2 130.46 Mbp_chr2_130463888_R | GTCTCGTGGGCTCGGATGATGATACCGCCGTGCTC | 60.3 | 0.94 |
| mm1__chr2 130.08 Mbp_chr2_130075316_F | TCGTCGGCAGCGTCGATGGAAGGGAAGACAGCCC | 60.1 | 0.94 |
| mm1__chr2 130.08 Mbp_chr2_130075316_R | GTCTCGTGGGCTCGGATACCGCTGTGCTCGTCATT | 59.8 | 0.94 |
| mm1__chr14 19.40 Mbp_chr14_19402853_F | TCGTCGGCAGCGTCATCATGTTCTGGTGCCTGGG | 60.0 | 0.94 |
| mm1__chr14 19.40 Mbp_chr14_19402853_R | GTCTCGTGGGCTCGGATACTGCCGTGCTCGTCATT | 59.8 | 0.94 |
| mm1__chr14 19.00 Mbp_chr14_18998899_F | TCGTCGGCAGCGTCATACTGCCGTGCTCGTCATT | 59.8 | 0.94 |
| mm1__chr14 19.00 Mbp_chr14_18998899_R | GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG | 60.0 | 0.94 |
| mm1__chr9 100.73 Mbp_chr9_100731271_F | TCGTCGGCAGCGTCGCCAGCTCACCATGGATGAT | 60.1 | 0.86 |
| mm1__chr9 100.73 Mbp_chr9_100731271_R | GTCTCGTGGGCTCGGCTAGGGAGCTCCACGATGGA | 60.4 | 0.86 |
| mm1__chr1 223.86 Mbp_chr1_223864796_F | TCGTCGGCAGCGTCCATGGAAGGGAAGATGGCCC | 60.4 | 0.86 |
| mm1__chr1 223.86 Mbp_chr1_223864796_R | GTCTCGTGGGCTCGGGCCAGCTCACCATGGATGAT | 60.1 | 0.86 |
| mm2__chr22 41.08 Mbp_chr22_41075184_F | TCGTCGGCAGCGTCATGCCCACCATCATGACCTG | 60.1 | 0.81 |
| mm2__chr22 41.08 Mbp_chr22_41075184_R | GTCTCGTGGGCTCGGTATTGCTGCGCTCGTTGTTG | 59.8 | 0.81 |
| mm1__chr19 9.52 Mbp_chr19_9518181_F | TCGTCGGCAGCGTCGCTGTCAGCTCACCATGGAT | 60.1 | 0.80 |
| mm1__chr19 9.52 Mbp_chr19_9518181_R | GTCTCGTGGGCTCGGCCACGATGGAGGGGAAGATG | 59.8 | 0.80 |
| mm2__chrX 46.29 Mbp_chrX_46289073_F | TCGTCGGCAGCGTCTGGAGGGGAAGACAACTGGG | 61.1 | 0.79 |
| mm2__chrX 46.29 Mbp_chrX_46289073_R | GTCTCGTGGGCTCGGCTCCATCACCAGTCCACACC | 60.0 | 0.79 |
| mm3__chr5 80.30 Mbp_chr5_80302792_F | TCGTCGGCAGCGTCATGATGAAGGGGAAGGCAGC | 60.1 | 0.75 |
| mm3__chr5 80.30 Mbp_chr5_80302792_R | GTCTCGTGGGCTCGGAGCTCACCATGCATGACGAT | 59.8 | 0.75 |
| mm2__chr18 62.44 Mbp_chr18_62442040_F | Primer3: not found at this Tm | N.d. | 0.75 |
| mm2__chr18 62.44 Mbp_chr18_62442040_R | Primer3: not found at this Tm | N.d. | 0.75 |
| mm2__chr15 43.99 Mbp_chr15_43990129_F | TCGTCGGCAGCGTCCATCGTCACCTGCAAAGCTG | 59.8 | 0.75 |
| mm2__chr15 43.99 Mbp_chr15_43990129_R | GTCTCGTGGGCTCGGATCACCATGCTTGTCGTCGA | 59.7 | 0.75 |
| mm4__chr13 47.12 Mbp_chr13_47118582_F | Primer3: not found at this Tm | N.d. | 0.64 |
| mm4__chr13 47.12 Mbp_chr13_47118582_R | Primer3: not found at this Tm | N.d. | 0.64 |
| mm3__chr11 1.80 Mbp_chr11_1804040_F | TCGTCGGCAGCGTCCACGATGGAGGGGAAGACG | 59.8 | 0.55 |
| mm3__chr11 1.80 Mbp_chr11_1804040_R | GTCTCGTGGGCTCGGCCCAGCTCACCATGGATGAT | 59.5 | 0.55 |
| mm2__chr5 98.34 Mbp_chr5_98338775_F | TCGTCGGCAGCGTCGCAGCTAGCTCACCATGGAT | 59.8 | 0.47 |
| mm2__chr5 98.34 Mbp_chr5_98338775_R | GTCTCGTGGGCTCGGGCATTGTCACCTGTGAAGCC | 59.7 | 0.47 |
| mm4__chr8 35.75 Mbp_chr8_35745491_F | TCGTCGGCAGCGTCCCAAGGAGCCTTAAATGCCC | 58.8 | 0.41 |
| mm4__chr8 35.75 Mbp_chr8_35745491_R | GTCTCGTGGGCTCGGTGTCATTGCATCAGTGTACGC | 59.2 | 0.41 |
| mm4__chr8 107.80 Mbp_chr8_107798187_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4__chr8 107.80 Mbp_chr8_107798187_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4__chr1 2.17 Mbp_chr1_2172168_F | TCGTCGGCAGCGTCGGACGGGCACATCAAGCT | 60.0 | 0.30 |
| mm4__chr1 2.17 Mbp_chr1_2172168_R | GTCTCGTGGGCTCGGCTTGTAGACACTACCCCGCG | 60.1 | 0.30 |
| mm4__chr12 27.78 Mbp_chr12_27780428_F | TCGTCGGCAGCGTCGAGCAGAGCGACTACTTCCG | 60.2 | 0.30 |
| mm4__chr12 27.78 Mbp_chr12_27780428_R | GTCTCGTGGGCTCGGGCGTTGATGAAGTCCAGCAC | 59.8 | 0.30 |
| mm4__chr20 21.04 Mbp_chr20_21044449_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4__chr20 21.04 Mbp_chr20_21044449_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm3__chr6 88.28 Mbp_chr6_88276931_F | TCGTCGGCAGCGTCCCCACAATGGAGGGGAAGAC | 60.0 | 0.21 |
| mm3__chr6 88.28 Mbp_chr6_88276931_R | GTCTCGTGGGCTCGGGCCAGCTCACCATGGATGAT | 60.1 | 0.21 |
| mm2__chr5 61.83 Mbp_chr5_61832990_F | TCGTCGGCAGCGTCGACGTTGTGTCCAGCAAAGC | 60.3 | 0.20 |
| mm2__chr5 61.83 Mbp_chr5_61832990_R | GTCTCGTGGGCTCGGTCCTTGGCCAAAGCAGTGAA | 60.1 | 0.20 |
| mm2__chr20 1.16 Mbp_chr20_1160615_F | TCGTCGGCAGCGTCGCTCCTCCACCGATCACAAT | 59.8 | 0.20 |
| mm2__chr20 1.16 Mbp_chr20_1160615_R | GTCTCGTGGGCTCGGGGGAAACATGGTTCCAGGGG | 60.6 | 0.20 |
| mm2__chr1 237.93 Mbp_chr1_237926862_F | TCGTCGGCAGCGTCCTCCTCCACCAGTCACGATG | 59.8 | 0.20 |
| mm2__chr1 237.93 Mbp_chr1_237926862_R | GTCTCGTGGGCTCGGTGGAGGGAAACACAGCTTGG | 60.1 | 0.20 |
| mm2__chr1 103.57 Mbp_chr1_103569584_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm2__chr1 103.57 Mbp_chr1_103569584_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm1__chr17 81.51 Mbp_chr17_81512299_F | TCGTCGGCAGCGTCCCATCCACTCACCTGGTGTC | 60.0 | 0.20 |
| mm1__chr17 81.51 Mbp_chr17_81512299_R | GTCTCGTGGGCTCGGATGGAAGAAGAGATCGCCGC | 60.2 | 0.20 |
| mm3__chr8 84.95 Mbp_chr8_84948621_F | TCGTCGGCAGCGTCCTGCTTTGCCACTGGTTCAC | 59.9 | 0.19 |
| mm3__chr8 84.95 Mbp_chr8_84948621_R | GTCTCGTGGGCTCGGGAAAGGAAGAAGGCCTGGGG | 60.3 | 0.19 |
| mm3__chr5 15.45 Mbp_chr5_15450731_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm3__chr5 15.45 Mbp_chr5_15450731_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm2__chrX 53.14 Mbp_chrX_53143858_F | TCGTCGGCAGCGTCGATGGAGGGGAACACAGCTC | 60.1 | 0.19 |
| mm2__chrX 53.14 Mbp_chrX_53143858_R | GTCTCGTGGGCTCGGTGCTCTTCCACAGGTTGCAA | 60.1 | 0.19 |
| mm4__chr5 138.28 Mbp_chr5_138283423_F | TCGTCGGCAGCGTCTAGGGCAAGAGCATGTGCAA | 59.9 | 0.18 |
| mm4__chr5 138.28 Mbp_chr5_138283423_R | GTCTCGTGGGCTCGGAAAGTGAGTGGGAGGGAAGG | 58.9 | 0.18 |
| mm3__chr12 120.35 Mbp_chr12_120353789_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3__chr12 120.35 Mbp_chr12_120353789_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr12 110.80 Mbp_chr12_110803125_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr12 110.80 Mbp_chr12_110803125_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr3 180.82 Mbp_chr3_180820575_F | TCGTCGGCAGCGTCAATGATATCGCCACGCTCGT | 59.9 | 0.17 |
| mm4__chr3 180.82 Mbp_chr3_180820575_R | GTCTCGTGGGCTCGGTTCTTCTGACCCATGCCCAC | 59.9 | 0.17 |
| mm4__chr3 49.17 Mbp_chr3_49171474_F | TCGTCGGCAGCGTCCCAGTGTCCAAGTGTCCGTT | 60.1 | 0.16 |
| mm4__chr3 49.17 Mbp_chr3_49171474_R | GTCTCGTGGGCTCGGGGAAACCTCCTCTCGCCTTA | 58.8 | 0.16 |
| mm3__chr2 73.90 Mbp_chr2_73901346_F | TCGTCGGCAGCGTCGTGTGAAGAGGAGACCACCG | 60.0 | 0.16 |
| mm3__chr2 73.90 Mbp_chr2_73901346_R | GTCTCGTGGGCTCGGCCCACAATGGAGGGGAAGAC | 60.0 | 0.16 |
| mm4__chr3 59.58 Mbp_chr3_59579128_F | TCGTCGGCAGCGTCTGCACTGATGTGAAGCCCAT | 59.9 | 0.14 |
| mm4__chr3 59.58 Mbp_chr3_59579128_R | GTCTCGTGGGCTCGGAGCATTCTGATCCGTTTGGC | 58.9 | 0.14 |
| mm4__chr12 14.37 Mbp_chr12_14365639_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr12 14.37 Mbp_chr12_14365639_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr12 132.07 Mbp_chr12_132067382_F | TCGTCGGCAGCGTCCAGGACAGACCGTGGTGG | 59.6 | 0.12 |
| mm4__chr12 132.07 Mbp_chr12_132067382_R | GTCTCGTGGGCTCGGCCGGTACCTTCTGCTGGAC | 59.7 | 0.12 |
| mm4__chr2 67.82 Mbp_chr2_67823888_F | TCGTCGGCAGCGTCTTTGGCAGCCTCATGGTTCA | 60.1 | 0.12 |
| mm4__chr2 67.82 Mbp_chr2_67823888_R | GTCTCGTGGGCTCGGAAGAGCACTGTGGGTGATGG | 59.9 | 0.12 |
| mm1__chr9 6.83 Mbp_chr9_6834487_F | TCGTCGGCAGCGTCCCGCTACCAGCTCACCATAG | 59.9 | 0.12 |
| mm1__chr9 6.83 Mbp_chr9_6834487_R | GTCTCGTGGGCTCGGACACGATGAAGGGGAAGACG | 59.7 | 0.12 |
| mm4__chr2 57.76 Mbp_chr2_57764984_F | TCGTCGGCAGCGTCAGATTGCTGGCTCGTCAATGA | 60.0 | 0.11 |
| mm4__chr2 57.76 Mbp_chr2_57764984_R | GTCTCGTGGGCTCGGCTTCTTGCCCATGCCCACTA | 60.0 | 0.11 |
| mm4__chr3 89.59 Mbp_chr3_89589793_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr3 89.59 Mbp_chr3_89589793_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr2 211.78 Mbp_chr2_211778667_F | TCGTCGGCAGCGTCGGAACCGGCTGGACAGTTTA | 59.9 | 0.11 |
| mm4__chr2 211.78 Mbp_chr2_211778667_R | GTCTCGTGGGCTCGGGGAGCGAGAAGATGGTCAGG | 59.8 | 0.11 |
| mm4__chr2 202.62 Mbp_chr2_202619468_F | TCGTCGGCAGCGTCGGAGCGAGAAGATGGTCAGG | 59.8 | 0.11 |
| mm4__chr2 202.62 Mbp_chr2_202619468_R | GTCTCGTGGGCTCGGTCAATAGTAGAAGCCGGCGC | 60.2 | 0.11 |
| mm4__chr1 106.81 Mbp_chr1_106805614_F | TCGTCGGCAGCGTCCACCTGCTAAGTGGAGCGAG | 60.4 | 0.11 |
| mm4__chr1 106.81 Mbp_chr1_106805614_R | GTCTCGTGGGCTCGGGCTGGCGCTTGAATCAGCTA | 61.0 | 0.11 |
| mm4__chr13 111.35 Mbp_chr13_111346406_F | TCGTCGGCAGCGTCATCAGGGACAGCCAGCCAT | 61.0 | 0.11 |
| mm4__chr13 111.35 Mbp_chr13_111346406_R | GTCTCGTGGGCTCGGTTCCTTGGCAGCTCTGTCTG | 59.9 | 0.11 |
| mm3__chr9 135.73 Mbp_chr9_135728729_F | TCGTCGGCAGCGTCGCCCAGCTTGGAGAGACATC | 60.4 | 0.09 |
| mm3__chr9 135.73 Mbp_chr9_135728729_R | GTCTCGTGGGCTCGGTAACCAGACCCGGCAAAACA | 59.8 | 0.09 |
| mm3__chr5 131.66 Mbp_chr5_131660060_F | TCGTCGGCAGCGTCATGCCCATCATCACACCCTG | 60.1 | 0.09 |
| mm3__chr5 131.66 Mbp_chr5_131660060_R | GTCTCGTGGGCTCGGATTGCTGTGCTTGTCGTCGA | 60.6 | 0.09 |
| mm3__chr22 35.54 Mbp_chr22_35535674_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3__chr22 35.54 Mbp_chr22_35535674_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm2__chr6 46.21 Mbp_chr6_46205009_F | TCGTCGGCAGCGTCATGGAAGAAGAGATCCCCGC | 59.2 | 0.08 |
| mm2__chr6 46.21 Mbp_chr6_46205009_R | GTCTCGTGGGCTCGGATATGGCTCGGAGGGCATTG | 59.9 | 0.08 |
| mm4__chr5 135.07 Mbp_chr5_135066866_F | TCGTCGGCAGCGTCAGGGAGGCTGATGTGAAAGC | 60.0 | 0.08 |
| mm4__chr5 135.07 Mbp_chr5_135066866_R | GTCTCGTGGGCTCGGTCCTGGGGTTGGTGTATGGA | 60.1 | 0.08 |
| mm4__chr17 16.47 Mbp_chr17_16466367_F | TCGTCGGCAGCGTCCTTTCAGACTCGGCACCCAT | 60.0 | 0.08 |
| mm4__chr17 16.47 Mbp_chr17_16466367_R | GTCTCGTGGGCTCGGCAGGGACCACACCTGGATCT | 60.9 | 0.08 |
| mm4__chrX 52.96 Mbp_chrX_52959493_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrX 52.96 Mbp_chrX_52959493_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrX 52.90 Mbp_chrX_52896297_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrX 52.90 Mbp_chrX_52896297_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3__chrY 18.15 Mbp_chrY_18147915_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3__chrY 18.15 Mbp_chrY_18147915_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3__chrY 17.76 Mbp_chrY_17758070_F | TCGTCGGCAGCGTCAACGGTGGAGGGAAACATGG | 60.2 | 0.07 |
| mm3__chrY 17.76 Mbp_chrY_17758070_R | GTCTCGTGGGCTCGGTCCTCTGTCCCACTCCTCTG | 59.9 | 0.07 |
| mm4__chr4 180.31 Mbp_chr4_180311834_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr4 180.31 Mbp_chr4_180311834_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr6 169.65 Mbp_chr6_169648288_F | TCGTCGGCAGCGTCTCTACAGATGCCTTCCGTGC | 59.8 | 0.07 |
| mm4__chr6 169.65 Mbp_chr6_169648288_R | GTCTCGTGGGCTCGGCTGGCTCTTCTGCATTTGGC | 59.8 | 0.07 |
| mm4__chr3 46.88 Mbp_chr3_46875620_F | TCGTCGGCAGCGTCCACCACCTCCCTGGATTTCC | 60.0 | 0.06 |
| mm4__chr3 46.88 Mbp_chr3_46875620_R | GTCTCGTGGGCTCGGTGCAGCGTCGTAACAGTTCT | 59.9 | 0.06 |
| mm4__chr10 5.59 Mbp_chr10_5585443_F | TCGTCGGCAGCGTCTTCTGAGGACTCACAGCTGC | 59.6 | 0.05 |
| mm4__chr10 5.59 Mbp_chr10_5585443_R | GTCTCGTGGGCTCGGGAAGGGAGACAAGGGTGGTG | 59.9 | 0.05 |
| mm3__chrX 75.76 Mbp_chrX_75757960_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3__chrX 75.76 Mbp_chrX_75757960_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr3 169.77 Mbp_chr3_169769465_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr3 169.77 Mbp_chr3_169769465_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr19 31.57 Mbp_chr19_31566196_F | TCGTCGGCAGCGTCTTCACCAGAGCCCAACGTAC | 59.9 | 0.05 |
| mm4__chr19 31.57 Mbp_chr19_31566196_R | GTCTCGTGGGCTCGGCCCTACCCTATGCCCCAGTA | 59.8 | 0.05 |
| mm4__chr8 138.19 Mbp_chr8_138192551_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr8 138.19 Mbp_chr8_138192551_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr20 59.74 Mbp_chr20_59737337_F | TCGTCGGCAGCGTCTCTGTGGGGATCCTGGTTCA | 60.1 | 0.04 |
| mm4__chr20 59.74 Mbp_chr20_59737337_R | GTCTCGTGGGCTCGGTGCAGCTGTATCTCCAGTGC | 60.1 | 0.04 |
| mm4__chr1 248.18 Mbp_chr1_248176264_F | TCGTCGGCAGCGTCCAGTGACTTCTCAATTTCCGACC | 59.5 | 0.04 |
| mm4__chr1 248.18 Mbp_chr1_248176264_R | GTCTCGTGGGCTCGGTCAGATTTCATGACAGTGTGGTG | 58.9 | 0.04 |
| mm3__chrX 122.37 Mbp_chrX_122365273_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3__chrX 122.37 Mbp_chrX_122365273_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chrX 152.91 Mbp_chrX_152906792_F | TCGTCGGCAGCGTCGTCTGCTCAGCTCCTGGATG | 60.1 | 0.02 |
| mm4__chrX 152.91 Mbp_chrX_152906792_R | GTCTCGTGGGCTCGGGGCTTGTCTCAGTGCATGTG | 59.4 | 0.02 |
| mm4__chr1 226.77 Mbp_chr1_226771924_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr1 226.77 Mbp_chr1_226771924_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr3 74.00 Mbp_chr3_74002249_F | TCGTCGGCAGCGTCAGAGCAGCCATGAACTGACA | 59.3 | 0.02 |
| mm4__chr3 74.00 Mbp_chr3_74002249_R | GTCTCGTGGGCTCGGTTGAGCAAAGTGTTGGTGCC | 59.5 | 0.02 |
| mm4__chr18 10.33 Mbp_chr18_10330721_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr18 10.33 Mbp_chr18_10330721_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr2 205.39 Mbp_chr2_205387855_F | TCGTCGGCAGCGTCTGGGTAATGGTTGCCAGTCC | 59.9 | 0.01 |
| mm4__chr2 205.39 Mbp_chr2_205387855_R | GTCTCGTGGGCTCGGAACTGGCAGCCTGTGTTTCT | 60.1 | 0.01 |
| mm4__chr9 69.43 Mbp_chr9_69425355_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr9 69.43 Mbp_chr9_69425355_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr16 28.32 Mbp_chr16_28320766_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr16 28.32 Mbp_chr16_28320766_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr19 45.03 Mbp_chr19_45033006_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr19 45.03 Mbp_chr19_45033006_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr12 1.57 Mbp_chr12_1570341_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr12 1.57 Mbp_chr12_1570341_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr20 56.27 Mbp_chr20_56271924_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr20 56.27 Mbp_chr20_56271924_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr19 42.04 Mbp_chr19_42042571_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr19 42.04 Mbp_chr19_42042571_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr7 157.09 Mbp_chr7_157087829_F | TCGTCGGCAGCGTCTGGCATACAGTAGGGCTTGC | 60.1 | 0.00 |
| mm4__chr7 157.09 Mbp_chr7_157087829_R | GTCTCGTGGGCTCGGCTGCCTTCCGGATATCCACG | 60.3 | 0.00 |
| mm4__chr7 101.09 Mbp_chr7_101088299_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr7 101.09 Mbp_chr7_101088299_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr4 53.48 Mbp_chr4_53477346_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr4 53.48 Mbp_chr4_53477346_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr2 65.33 Mbp_chr2_65334643_F | TCGTCGGCAGCGTCTGCTTTCCTTACTCCCCTGT | 58.2 | 0.00 |
| mm4__chr2 65.33 Mbp_chr2_65334643_R | GTCTCGTGGGCTCGGCACCAAAGCCAATCCAACGT | 59.3 | 0.00 |
| mm4__chr18 7.60 Mbp_chr18_7600890_F | TCGTCGGCAGCGTCAGGTGCTGTCAGGGTTTCAG | 59.8 | 0.00 |
| mm4__chr18 7.60 Mbp_chr18_7600890_R | GTCTCGTGGGCTCGGGGTGAGCCTGTTCTCAGTCC | 60.0 | 0.00 |
| mm4__chr17 21.70 Mbp_chr17_21702869_F | TCGTCGGCAGCGTCCAACCCCTACAGCATCGTGT | 60.0 | 0.00 |
| mm4__chr17 21.70 Mbp_chr17_21702869_R | GTCTCGTGGGCTCGGCTTCTTGACGAAGCGGTTGC | 60.1 | 0.00 |
| mm4__chr17 21.42 Mbp_chr17_21415425_F | TCGTCGGCAGCGTCCATCGTGTCATCGGAGGAGG | 59.9 | 0.00 |
| mm4__chr17 21.42 Mbp_chr17_21415425_R | GTCTCGTGGGCTCGGCTTCTTGACGAAGCGGTTGC | 60.1 | 0.00 |
| mm4__chr11 61.84 Mbp_chr11_61838394_F | TCGTCGGCAGCGTCGAGGTCTCATGGGCTGTTGT | 59.6 | 0.00 |
| mm4__chr11 61.84 Mbp_chr11_61838394_R | GTCTCGTGGGCTCGGGGTACCACTCCTCTCCTTGT | 58.0 | 0.00 |
| mm1__chr20 38.62 Mbp_chr20_38620718_F | TCGTCGGCAGCGTCTGGGACTTGGTCTCCAGTGA | 60.1 | 0.00 |
| mm1__chr20 38.62 Mbp_chr20_38620718_R | GTCTCGTGGGCTCGGAGAGCCTGAAGGAAGGGACA | 60.1 | 0.00 |
Primers underlined, off-targets in bold.
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529602 |
CACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGAGCCGTTGTCGACGA CGAGCGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC |
| mm0__chr5 77.78 Mbp_chr5_77784918 |
ATATCACCGCGCTCGTCATTGACAACGGCTCCGGCATGTGCAAGGCCAGCTTCACGGGCGACAATGCCGCCCGGGCAGTC TTCCCCTCCATCGTT |
| mm1__chr22 15.72 Mbp_chr22_15721518 |
ATACTGCCGTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTC TTCCCTTCCATCGTGGGGTGCCCCAGGCACCAGAACATGAT |
| mm1__chr2 131.63 Mbp_chr2_131626170 |
ATACCGCTGTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCAGGCTGTC TTCCCTTCTATCGTGGGGCGCCCCAGGCACCAGGGCATGATGGAGGGCATGCATCAGAAG |
| mm1__chr2 131.26 Mbp_chr2_131263587 |
TGGATGATGATACCGCCGTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCC CGGGCTGTCTTCCCTTCCATC |
| mm1__chr2 130.66 Mbp_chr2_130656781 |
ATACCGCTGTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTC TTCCCTTCCATC |
| mm1__chr2 130.46 Mbp_chr2_130463888 |
GATGGAAGGGAAGACAGCCCGGGGGGCATCGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGA GCACGGCGGTATCATCAT |
| mm1__chr2 130.08 Mbp_chr2_130075316 |
GATGGAAGGGAAGACAGCCCGGGGGGCATCGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGA GCACAGCGGTAT |
| mm1__chr14 19.40 Mbp_chr14_19402853 |
ATCATGTTCTGGTGCCTGGGGCACCCCACGATGGAAGGGAAGACAGCCCGGGGGGCATCGTCACCTGCAAAGCCGGCCTT GCACATGCCAGAGCCGTTGTCAATGACGAGCACGGCAGTAT |
| mm1__chr14 19.00 Mbp_chr14_18998899 |
ATACTGCCGTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTC TTCCCTTCCATCGTGGGGTGCCCCAGGCACCAGAACATGAT |
| mm1__chr9 100.73 Mbp_chr9_100731271 |
GCCAGCTCACCATGGATGATGATATCGCCCCACTTGTCATTGACAACGGCTCAGGCATGTGCAAGGCCGGCTTTGCAGGT GATAATGTCCCCCTGGGCCATCTTCCCCCTCCATCGTGGAGCTCCCTAG |
| mm1__chr1 223.86 Mbp_chr1_223864796 |
CATGGAAGGGAAGATGGCCCAGGGGTCATCATCCCCTGTGAAGCCAGCCTTGCACATGCCGGAGCTGTTGTCAATGACGA GCGCCGCGATATCATCATCCATGGTGAGCTGGC |
| mm2__chr22 41.08 Mbp_chr22_41075184 |
ATGCCCACCATCATGACCTGGTGCCTGGTGCACCCCACGATGGAAGGGAAGATGGCCCAGGGGACATCGTCACCCGCGAA GCCGGCCTTGCACATGCCAGAGCTGTTGTCAACAACGAGCGCAGCAATA |
| mm1__chr19 9.52 Mbp_chr19_9518181 |
GCTGTCAGCTCACCATGGATGATGATATTGAGGTGCTTGTAGTTAACAATGGCTCCGGCATGTGCAAGGTCAACTTCGTG GGCGACAATGCCCCCCGGGCCATCTTCCCCTCCATCGTGG |
| mm2__chrX 46.29 Mbp_chrX_46289073 |
TGGAGGGGAAGACAACTGGGGGGCATCATCGCCCATGAAGGCAGCCTTGCACATGCCAGAGCCTTTGTTGAAGACGAGAG CAGTGATATCATCATTCATGGTGATCTGGCAGTGGGTGTGGACTGGTGATGGAG |
| mm3__chr5 80.30 Mbp_chr5_80302792 |
ATGATGAAGGGGAAGGCAGCCCGGGGGACACCATCGCTCGCAAAGCTAGCCTTGCATATGCCAGAGCCATTGTTGAATAC GAGAGCAGTGATATCGTCATGCATGGTGAGCT |
| mm2__chr15 43.99 Mbp_chr15_43990129 | CATCGTCACCTGCAAAGCTGGCCTTGCACATGCCAGAGCCATTGTCGACGACAAGCATGGTGAT |
| mm3__chr11 1.80 Mbp_chr11_1804040 |
CACGATGGAGGGGAAGACGGCCCAGGGGGCATCGTCGTCGGTGAAGCTGGCCTTGCACATGCCAGAGGTGTTGTCTATGA CAAGCAAGGTGATATTATCATCCATGGTGAGCTGGG |
| mm2__chr5 98.34 Mbp_chr5_98338775 |
GCAGCTAGCTCACCATGGATGATGATATCAGCACGCTCCTCATCAACAAGGGCTCTGGCATGTGCAAGGCCGGCTTCACA GGTGACAATGC |
| mm4__chr8 35.75 Mbp_chr8_35745491 |
CCAAGGAGCCTTAAATGCCCACCATGCACATGCCTAAGGCATTGAGAATTTAAGTCAAAGCGTACACTGATGCAATGACA |
| mm4__chr1 2.17 Mbp_chr1_2172168 |
GGACGGGCACATCAAGCTCACAGACTACGGCATGTGCAAGGTGCGTGCCTTGGACCGCCTCCCCTGACCATCCCGCATGT GCGTCTCGGGGCGCCTGTCCCGCGGGGTAGTGTCTACAAG |
| mm4__chr12 27.78 Mbp_chr12_27780428 |
GAGCAGAGCGACTACTTCCGCGCCCTCTACCGCTCCGGCATGCGCGAGGCCCTGAGCCAGGAGGCCGGCGGCCCGGAGGT GCAGCAGCTGCGCGGCCTCAGCGCGCCGGGCCTGCGGCTGGTGCTGGACTTCATCAACGC |
| mm3__chr6 88.28 Mbp_chr6_88276931 |
CCCACAATGGAGGGGAAGACGGCCTGGGGAGCCTTGCGCATGCTGGAGCAGTTGTCGACGACGACGAGCGCGGTGATAGC ATCATCCATGGTGAGCTGGC |
| mm2__chr5 61.83 Mbp_chr5_61832990 |
GACGTTGTGTCCAGCAAAGCCAGCTTTGCACATGCTGGAGCCATTGTCAATGACCAGCCCATGATCTCTTCTTTCACTGC TTTGGCCAAGGA |
| mm2__chr20 1.16 Mbp_chr20_1160615 |
GCTCCTCCACCGATCACAATGGAAGAAGAGATCACCATACTCGTCATTGACAATGGCTCCAGCATGTGCAAAGCTGGTTT TGCTGGGGACGACGCCCCTGGAACCATGTTTCCC |
| mm2__chr1 237.93 Mbp_chr1_237926862 |
CTCCTCCACCAGTCACGATGGAAGAAGAGATCGCCACGCTTGCCATTGACAATGGCTCCAGCATGTGCAAAGCTGGCTTT GCTGGGGACGATGCCCCCCAAGCTGTGTTTCCCTCCA |
| mm1__chr17 81.51 Mbp_chr17_81512299 |
CCATCCACTCACCTGGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCGTCCCCAGCAAAACCAG CTTTGCACATGCCGGAGCCATTGTCAATGACCAGCGCGGCGATCTCTTCTTCCAT |
| mm3__chr8 84.95 Mbp_chr8_84948621 |
CTGCTTTGCCACTGGTTCACATCTGCCCTGAGCTCACCATGGATGATGATACCTCTGCACTTGTTGGCAACTGCTCTGGT ATGTGCAAGGCCAGAATCCTTGTCAAAGATGCCCCCAGGCCTTCTTCCTTTC |
| mm2__chrX 53.14 Mbp_chrX_53143858 |
GATGGAGGGGAACACAGCTCAGGGGCCATCCATCCCCAGCAAAGCCAGCTTTGCACATGCCAGAGCCATTGTCAATGATG AGCGCGGCGATCTCTATTTCCATTGCAACCTGTGGAAGAGCA |
| mm4__chr5 138.28 Mbp_chr5_138283423 |
TAGGGCAAGAGCATGTGCAAACCTTGTCAAATAAAAAAATTAAAAGCCAGTCCAACCACTCAGGCACGTGCATGGGCCGC CCTATGGCTACAACCCCCCTTCCCTCCCACTCACTTT |
| mm4__chr3 180.82 Mbp_chr3_180820575 |
AATGATATCGCCACGCTCGTCATGGACAATGGCTCTCCCATGTGCAAGGCCAGCTTAGCAGGCGACGATGCCCCTCCATC GTGAGGCACCCATGGCACCAGGGCATGATCGTGGGCATGGGTCAGAAGAA |
| mm4__chr3 49.17 Mbp_chr3_49171474 |
CCAGTGTCCAAGTGTCCGTTTATCTGTCCGTTTGCATATGACCGTAGATCGGCACGCCCTGCTCCAGCTGCGGCATGTGC AGGGTTAAGGCGAGAGGAGGTTTCC |
| mm3__chr2 73.90 Mbp_chr2_73901346 |
GTGTGAAGAGGAGACCACCGCGCTCGTGTGTGACAATGGCTCTGGCCTGTGCAAGGCAGGCTTCGCAGGAGATGATGCCC CCCGGGCTGTCTTCCCCTCCATTGTGGG |
| mm4__chr3 59.58 Mbp_chr3_59579128 |
TGCACTGATGTGAAGCCCATCTGGGATCTCTAGACACAAGGCCACCGGCATGAGCATGGCAGCCCAGGGAGAATACAGAT GGTGTACCTCTATGCCAAACGGATCAGAATGCT |
| mm4__chr12 132.07 Mbp_chr12_132067382 |
CAGGACAGACCGTGGTGGCCCAGCCCGTGCACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN GCCATCCAGCCCCAGGCTGCACAGGGCCCGGCAGCCGTCCAGCAGAAGGTACCGG |
| mm4__chr2 67.82 Mbp_chr2_67823888 |
TTTGGCAGCCTCATGGTTCAGCAATACCAGTGGTCCCTCACCTTTGGCTTCGGGCGCCGTGCACAAGCCGGCTCCGGTGA GCCACCAGCCATCACCCACAGTGCTCTT |
| mm1__chr9 6.83 Mbp_chr9_6834487 |
CCGCTACCAGCTCACCATAGATGATGATATTGCTACGCCCGTCGTCGACAACGGCTCCGGCATGTGGAAGGCTGGCTTCG CAGGTGACGATGACCCCCGGCCGTCTTCCCCTTCATCGTGT |
| mm4__chr2 57.76 Mbp_chr2_57764984 |
AGATTGCTGGCTCGTCAATGATAGTGGCTCTGGCATGTGCAAAGCTGGCTTTGCTGGGGAAGACACCCCCTGAGCCATGT TTCTCTCCATCGTCAGGTGCCCCTGGCACCACAGTGTGATAGTGGGCATGGGCAAGAAG |
| mm4__chr2 211.78 Mbp_chr2_211778667 |
GGAACCGGCTGGACAGTTTATCCCCCTTTAGCAGGAAACCTAGCACATGCAGGAGCCTCTGTGAACCTGACCATCTTCTC GCTCC |
| mm4__chr2 202.62 Mbp_chr2_202619468 |
GGAGCGAGAAGATGGTCAGGTCCACAGAGGCTCCTGCATGTGCTAGGTTTCCTCTTAAAGGGGGATAAACCGTCCAGCCG GTTCCAGCGCCGGCTTCTACTATTGA |
| mm4__chr1 106.81 Mbp_chr1_106805614 |
CACCTGCTAAGTGGAGCGAGAAGATGATCAGATCCAGAGGCTCCTGCATGTGCTAGGTTTCCTGCTAAAGGGGGATAAAC TGTTTAGCTGATTCAAGCGCCAGC |
| mm4__chr13 111.35 Mbp_chr13_111346406 |
ATCAGGGACAGCCAGCCATGGCCCGGATGTGTGACAACCCTGCACAGGCCGGGGCCTCTGCCCTCGCGCAGTTGGTGCCC TGCTGAGCGAGGCGAAGGCCAGACAGAGCTGCCAAGGAA |
| mm3__chr9 135.73 Mbp_chr9_135728729 |
GCCCAGCTTGGAGAGACATCTCCCCAGCGGCCCCGGCATGTGCTCAGCAGCTGGTGAGCTCAATCCAGGAGTGTTTTGCC GGGTCTGGTTA |
| mm3__chr5 131.66 Mbp_chr5_131660060 |
ATGCCCATCATCACACCCTGGTGCCTCGGGTGCCCCATTATGGAGGGGAAGACGGCCCGGGGGCATCGTTGCCCGTGAAG CAGACCTTCCACATGCCAGAGCCATTGTCGACGACAAGCACAGCAAT |
| mm2__chr6 46.21 Mbp_chr6_46205009 |
ATGGAAGAAGAGATCCCCGCGCTCTTCATTGACAATGGCTCCGGCATGTGAAAAGCAGCTTTGCTGGGACAATGCCCTCC GAGCCATAT |
| mm4__chr5 135.07 Mbp_chr5_135066866 |
AGGGAGGCTGATGTGAAAGCCAGCATCCACCGGCCTCGGCATGTGCCAGGCTCTGTGCTTTCCATACACCAACCCCAGGA |
| mm4__chr17 16.47 Mbp_chr17_16466367 |
CTTTCAGACTCGGCACCCATGGCCTGCCCTCCTTCTCAGGAAGACACAGAATGGGGGTAGGCACACAAGGCCTGCTGCAT GTGCAAGGGAAGTGGAAGAATCAGATCCAGGTGTGGTCCCTG |
| mm3__chrY 17.76 Mbp_chrY_17758070 |
AACGGTGGAGGGAAACATGGCTTGGGGAGCATCGTCCCCTGCAAAGCCAGCTTTGCACATGCAAGAGCCATTGTCAATGA CGAGCGCAGCGATCTCTTCTTCTACTGCGATGGACAGAGGAGTGGGACAGAGGA |
| mm4__chr6 169.65 Mbp_chr6_169648288 |
TCTACAGATGCCTTCCGTGCACATGCCAATGCCTTCCGTGCACACGCCCGAGCCCTGGGTGGCATTTGGGGCCTCGCCAC CACTGCTACCCAGCAGCTCCCAGCAGAAGGCCCAAGAGAGCCAAATGCAGAAGAGCCAG |
| mm4__chr3 46.88 Mbp_chr3_46875620 | CACCACCTCCCTGGATTTCCCAACTGCTCCACAATGTGCATGGGGCAAGAACTGTTACGACGCTGCA |
| mm4__chr10 5.59 Mbp_chr10_5585443 |
TTCTGAGGACTCACAGCTGCTGAGGAAACACACTTCCACTACCAGCTTCACACCCAGCACGTGCGGGAGCCGTGGAAAGC CACCACCCTTGTCTCCCTTC |
| mm4__chr19 31.57 Mbp_chr19_31566196 |
TTCACCAGAGCCCAACGTACAGCGGCAGCTGGCAGAGAAGTGAGTGCGGGAGCACTTGATTGAATCTTCATGTAGCCAGG CCCTCCACATGCCTGAGCCACTGTACTGGGGCATAGGGTAGGG |
| mm4__chr20 59.74 Mbp_chr20_59737337 |
TCTGTGGGGATCCTGGTTCAGGGCTCTCTATGTCCCCCTGGCACGTGCCTGAGCTGTTGCTGGCACTGGAGATACAGCTG CA |
| mm4__chr1 248.18 Mbp_chr1_248176264 |
CAGTGACTTCTCAATTTCCGACCATGATTTTCATGGTTTCTGTGTTACTATTGCTCTGGCATGTGCACAGCACCACACTG TCATGAAATCTGA |
| mm4__chrX 152.91 Mbp_chrX_152906792 |
GTCTGCTCAGCTCCTGGATGCAAAGCATCCCAATTCACCTTTGGGAAAGAAGCCCTTCTGCTTGCACCTCTGCCTGTGGC ACACCTGCTCCTGCTTGTGCATGGCGCCCAGTGCCCAGCACACATGCACTGAGACAAGCC |
| mm4__chr3 74.00 Mbp_chr3_74002249 |
AGAGCAGCCATGAACTGACAACTCCCAGTAAAGCAGACACTCTGCACATGCCAGTGCAGGTGGGTGGCACCAACACTTTG CTCAA |
| mm4__chr2 205.39 Mbp_chr2_205387855 |
TGGGTAATGGTTGCCAGTCCAGCCGTCATCTGAGTGACGCTGCTGGTGTTCCCTGCACCTTCCAGAGCCGTTCAGAAACA CAGGCTGCCAGTT |
| mm4__chr7 157.09 Mbp_chr7_157087829 |
TGGCATACAGTAGGGCTTGCTATTGACGATGAGCGCACCCACGGCTCCGGCAACTACAAGGCGGTGCTCACGGACAGCAC GGCCCTCGGCGTGGATATCCGGAAGGCAG |
| mm4__chr2 65.33 Mbp_chr2_65334643 |
TGCTTTCCTTACTCCCCTGTCAAAGGCTCGGGCATCAGCAGGGCTTTGGAAAGTAAGTCCAAACTTCCTATTATCGACCT TCCAGTGATGAAACGTTGGATTGGCTTTGGTG |
| mm4__chr18 7.60 Mbp_chr18_7600890 | AGGTGCTGTCAGGGTTTCAGAGGAAACTGCTCCTGCATCTGCAGGGAGCTCTGCTGGACTGAGAACAGGCTCACC |
| mm4__chr17 21.70 Mbp_chr17_21702869 |
CAACCCCTACAGCATCGTGTCATTGGAGGAGGACGGGCTGCACCTGGTCACCATGTCGGGNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNCGGCGCAGGTGCCGCAACCGCTTCGTCAAGAAG |
| mm4__chr17 21.42 Mbp_chr17_21415425 |
CATCGTGTCATCGGAGGAGGACGGGCTGCACCTGGTCACCATGTCGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNCGGCGCAGGTGCCGCAACCGCTTCGTCAAGAAG |
| mm4__chr11 61.84 Mbp_chr11_61838394 |
GAGGTCTCATGGGCTGTTGTTTTGTTCAACAATGAAGTCTCCATGTAAATGGCGGAGCCGTTTATAGAAGAAAGTTNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGAGTTGTTACAAGGAGAGGAGTGGTACC |
| mm1__chr20 38.62 Mbp_chr20_38620718 |
TGGGACTTGGTCTCCAGTGAAGCTTCTGCTCTGTGCAAATGCCGGAGCCGTTGCCAGGAGGTTGTGCCCTGTCCCTTCCT TCAGGCTCT |