CRISPOR

Micro-homology scoring of potential deletions

Guide: CGCGGGCGACGATGCCCCCC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	(Wild-type sequence)
397.6	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCC--------------TCCATCGTGGGGCGCCCCAGGCACCA`	`14 bp deleted → frameshift`
382.8	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCC---------GTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`9 bp deleted → no frameshift`
377.6	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC---------------TCCATCGTGGGGCGCCCCAGGCACCA`	`15 bp deleted → no frameshift`
359.2	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCC----------------TCCATCGTGGGGCGCCCCAGGCACCA`	`16 bp deleted → frameshift`
341.6	`GGCATGTGCAAGGCCGGCTTCGCGGGC-----------------CGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`17 bp deleted → frameshift`
313.2	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCC-------------CTCCATCGTGGGGCGCCCCAGGCACCA`	`13 bp deleted → frameshift`
311.6	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCC-----GTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`5 bp deleted → frameshift`
296.4	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC------GTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`6 bp deleted → no frameshift`
282.0	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCC-------GTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`7 bp deleted → frameshift`
268.0	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCC--------GTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`8 bp deleted → frameshift`
256.2	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCC-----------------TCCATCGTGGGGCGCCCCAGGCACCA`	`17 bp deleted → frameshift`
223.0	`GGCATGTGCAAGGCCG------------------------------TCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`30 bp deleted → no frameshift`
219.6	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCC------------CCTCCATCGTGGGGCGCCCCAGGCACCA`	`12 bp deleted → no frameshift`
188.8	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACG---------------TCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`15 bp deleted → no frameshift`
183.0	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCC----------------------------------AGGCACCA`	`34 bp deleted → frameshift`
179.6	`GGCATGTGCAAGGCCGGCTTCGCGGG----------------GCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`16 bp deleted → frameshift`
170.8	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCC-----------------ATCGTGGGGCGCCCCAGGCACCA`	`17 bp deleted → frameshift`
163.8	`GGCATGTGCAAGGCCGGC--------------------------CGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`26 bp deleted → frameshift`
162.8	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC------------------ATCGTGGGGCGCCCCAGGCACCA`	`18 bp deleted → no frameshift`
162.8	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCC------------------TCCATCGTGGGGCGCCCCAGGCACCA`	`18 bp deleted → no frameshift`
162.8	`GGCATGTGCAAGGCCGGCTTCGCGGGCG------------------TCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`18 bp deleted → no frameshift`
162.8	`GGCATGTGCAAGGCCGGCTTCGCGG------------------CCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`18 bp deleted → no frameshift`
161.6	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCC--------------------------------AGGCACCA`	`32 bp deleted → frameshift`
154.8	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCC-------------------ATCGTGGGGCGCCCCAGGCACCA`	`19 bp deleted → frameshift`
153.6	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC---------------------------------AGGCACCA`	`33 bp deleted → no frameshift`
147.2	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCC--------------------ATCGTGGGGCGCCCCAGGCACCA`	`20 bp deleted → frameshift`
140.0	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCC---------------------ATCGTGGGGCGCCCCAGGCACCA`	`21 bp deleted → no frameshift`
140.0	`GGCATGTGCAAGGCCGGCTTCGC---------------------CGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`21 bp deleted → no frameshift`
135.0	`GGCATGTGCAAGGCCGGCTTCGCGGGCG----------------------------------------CCCCAGGCACCA`	`40 bp deleted → frameshift`
133.8	`GGCATGTGCAAGGCCGGCTTC------------------------------CCCTCCATCGTGGGGCGCCCCAGGCACCA`	`30 bp deleted → no frameshift`
133.2	`GGCATGTGCAAGGCCGGCTTCGCG----------------------TCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`22 bp deleted → frameshift`
127.2	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCC-------------------------------CAGGCACCA`	`31 bp deleted → frameshift`
120.4	`GGCATGTGCAAGGCCGGCTTCG------------------------TCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`24 bp deleted → no frameshift`
114.8	`GGCATGTGCAAGGCCGG-------------------------GCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`25 bp deleted → frameshift`
104.4	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCC-----------------------------------AGGCACCA`	`35 bp deleted → frameshift`
94.0	`GGCATGTGCAAGG-----------------------------GCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`29 bp deleted → frameshift`
89.2	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCC------------------------------CCAGGCACCA`	`30 bp deleted → no frameshift`
89.2	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACG------------------------------TGGGGCGCCCCAGGCACCA`	`30 bp deleted → no frameshift`
85.2	`GGCATGTGCAAGGCCGGCTTCGCGGG---------------------------------------GCGCCCCAGGCACCA`	`39 bp deleted → no frameshift`
80.8	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGC--------------------------------GCCCCAGGCACCA`	`32 bp deleted → frameshift`
77.7	`GGCATGTGCAAGGCCGGCTTC---------------------------TTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`27 bp deleted → no frameshift`
77.4	`GGC-----------------------------------------CGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`41 bp deleted → frameshift`
76.8	`GGCATGTGCAAGGCCGGCTTCGCGGGCG---------------------------------TGGGGCGCCCCAGGCACCA`	`33 bp deleted → no frameshift`
71.0	`GGCATGTGCAAGGCCGGCTTCG---------------------------------------TGGGGCGCCCCAGGCACCA`	`39 bp deleted → no frameshift`
70.5	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATG-----------------------------GGGCGCCCCAGGCACCA`	`29 bp deleted → frameshift`
69.6	`GGCATGTGC-----------------------------------CGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`35 bp deleted → frameshift`
66.6	`GGCATGTGCAAGGCCGGCTTCGCG--------------------------------------------CCCCAGGCACCA`	`44 bp deleted → frameshift`
66.0	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCC------------------------------------AGGCACCA`	`36 bp deleted → no frameshift`
62.8	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACG-------------------------------------CCCCAGGCACCA`	`37 bp deleted → frameshift`
62.8	`GGCATGTGCAAGGCCGGCTTCGCG-------------------------------------TGGGGCGCCCCAGGCACCA`	`37 bp deleted → frameshift`
62.8	`GGCATGTGCAAGGCC-------------------------------------CCTCCATCGTGGGGCGCCCCAGGCACCA`	`37 bp deleted → frameshift`
60.0	`GGCATGTGCAAGGCCGGCTTCGCGGG--------------------------------------GGCGCCCCAGGCACCA`	`38 bp deleted → frameshift`
60.0	`GGCATGTGCAAGGCCGGCTTCGC----------------------------------------------CCCAGGCACCA`	`46 bp deleted → frameshift`
60.0	`GGCATGTGCAAGGCC--------------------------------------CTCCATCGTGGGGCGCCCCAGGCACCA`	`38 bp deleted → frameshift`
56.8	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCC---------------------------------------A`	`39 bp deleted → no frameshift`
56.8	`GGCATGTGCAAGGCC---------------------------------------TCCATCGTGGGGCGCCCCAGGCACCA`	`39 bp deleted → no frameshift`
54.6	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT--------------------------CGTGGGGCGCCCCAGGCACCA`	`26 bp deleted → frameshift`
54.0	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC----------------------------------------A`	`40 bp deleted → frameshift`
54.0	`GG----------------------------------------GCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`40 bp deleted → frameshift`
52.2	`GGCATGTGCAAGGCCGGCTTC-----------------------------------CATCGTGGGGCGCCCCAGGCACCA`	`35 bp deleted → frameshift`
51.6	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCC-----------------------------------------A`	`41 bp deleted → frameshift`
51.6	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGC-----------------------------------------ACCA`	`41 bp deleted → frameshift`
51.6	`GGCATGTGCAAGGCCGGCTTCGCGG-----------------------------------------CGCCCCAGGCACCA`	`41 bp deleted → frameshift`
51.6	`GGCATGTGCAAGGCCGGCTTCGCGGGC-------------------------------------------------ACCA`	`49 bp deleted → frameshift`
51.6	`GGCATGTGCAAGGCCGGC-------------------------------------------------GCCCCAGGCACCA`	`49 bp deleted → frameshift`
49.5	`GGCATGTGCAAGGCCGGCT------------------------------------CCATCGTGGGGCGCCCCAGGCACCA`	`36 bp deleted → no frameshift`
48.8	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCC------------------------------------------A`	`42 bp deleted → no frameshift`
48.8	`GGCATGTGCAAGGCCGGCTTCGCGGGC------------------------------------------CCCAGGCACCA`	`42 bp deleted → no frameshift`
48.8	`GGCATGTGCAAGGCC------------------------------------------ATCGTGGGGCGCCCCAGGCACCA`	`42 bp deleted → no frameshift`
46.4	`GGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCC-------------------------------------------A`	`43 bp deleted → frameshift`
42.6	`GGCATGTGCAAGGC-----------------------------------------------------GCCCCAGGCACCA`	`53 bp deleted → frameshift`
42.0	`GGCATGTGCAAGGCCG---------------------------------------------TGGGGCGCCCCAGGCACCA`	`45 bp deleted → no frameshift`
40.5	`GGCATGT----------------------------------------CTTCCCCTCCATCGTGGGGCGCCCCAGGCACCA`	`40 bp deleted → frameshift`
38.4	`GGCATGTGCAAGGCC-------------------------------------------------------CCAGGCACCA`	`55 bp deleted → frameshift`
38.0	`GGCATGTGCAAGGCCGG-----------------------------------------------GGCGCCCCAGGCACCA`	`47 bp deleted → frameshift`
36.4	`GGCATGTGCAAGGCCGG------------------------------------------------GCGCCCCAGGCACCA`	`48 bp deleted → no frameshift`
33.0	`GGCATGTGCAAGGCCGGC----------------------------------------------------------ACCA`	`58 bp deleted → frameshift`
32.8	`GGCATGTGCAAGGCCGGCTTCGCGG--------------------------------------------------CACCA`	`50 bp deleted → frameshift`
32.0	`GGCATGTG-------------------------------------------------------GGGCGCCCCAGGCACCA`	`55 bp deleted → frameshift`
31.5	`GGCATGTGCAAGGC--------------------------------------------------------------ACCA`	`62 bp deleted → frameshift`
31.2	`GGCATGTGCAAGGCCGGC---------------------------------------------------CCCAGGCACCA`	`51 bp deleted → no frameshift`
31.2	`GGCATGTGCAAGG---------------------------------------------------GGCGCCCCAGGCACCA`	`51 bp deleted → no frameshift`
29.6	`GGCATGTGCAAGGCCG----------------------------------------------------CCCCAGGCACCA`	`52 bp deleted → frameshift`
29.6	`GGCATGTGCAAGG----------------------------------------------------GCGCCCCAGGCACCA`	`52 bp deleted → frameshift`
28.4	`GGCATGTGCAAGGCCGGCTTCGC-----------------------------------------------------ACCA`	`53 bp deleted → frameshift`
27.3	`GGCATGTGCAAGGCCGGCTTCGCGGGCGAC------------------------------------------------CA`	`48 bp deleted → no frameshift`
27.3	`GGCATGTGCA------------------------------------------------TCGTGGGGCGCCCCAGGCACCA`	`48 bp deleted → no frameshift`
26.8	`GGCAT------------------------------------------------------CGTGGGGCGCCCCAGGCACCA`	`54 bp deleted → no frameshift`
24.6	`GGC----------------------------------------------------------------GCCCCAGGCACCA`	`64 bp deleted → frameshift`
24.4	`GGCATGTGCAAGGCC--------------------------------------------------------CAGGCACCA`	`56 bp deleted → frameshift`
23.2	`GGCATGTGCAAGGCC---------------------------------------------------------AGGCACCA`	`57 bp deleted → no frameshift`
22.0	`GGCATGTGC----------------------------------------------------------GCCCCAGGCACCA`	`58 bp deleted → frameshift`
20.0	`GGCATGTGC------------------------------------------------------------CCCAGGCACCA`	`60 bp deleted → no frameshift`
18.2	`GGCA-------------------------------------------------------------------------CCA`	`73 bp deleted → frameshift`
18.0	`GG--------------------------------------------------------------GGCGCCCCAGGCACCA`	`62 bp deleted → frameshift`
17.5	`GGCATGTGCA-------------------------------------------------------------------CCA`	`67 bp deleted → frameshift`
17.4	`GGCATG---------------------------------------------------------GGGCGCCCCAGGCACCA`	`57 bp deleted → no frameshift`
17.2	`GG---------------------------------------------------------------GCGCCCCAGGCACCA`	`63 bp deleted → no frameshift`
16.4	`GGCATGTGCAAGGCC----------------------------------------------------------------A`	`64 bp deleted → frameshift`
14.8	`GGC------------------------------------------------------------------CCCAGGCACCA`	`66 bp deleted → no frameshift`
12.9	`GGCATGTGCA---------------------------------------------------------------GGCACCA`	`63 bp deleted → no frameshift`
9.6	`GGCA---------------------------------------------------------------------GGCACCA`	`69 bp deleted → no frameshift`
9.0	`GGCATGTGCA----------------------------------------------------------------------`	`70 bp deleted → frameshift`
6.6	`GGCA----------------------------------------------------------------------------`	`76 bp deleted → frameshift`

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