CRISPOR

Micro-homology scoring of potential deletions

Guide: GCCGGGCGCGCTGTGAGCCG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	(Wild-type sequence)
382.8	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCC---------CCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`9 bp deleted → no frameshift`
329.4	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCG------------CCCTGGCCACTTCCGGCGCGCCGAGTCC`	`12 bp deleted → no frameshift`
296.4	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCG------CCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`6 bp deleted → no frameshift`
283.2	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCC---------------CTGGCCACTTCCGGCGCGCCGAGTCC`	`15 bp deleted → no frameshift`
269.4	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGC----------------CCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`16 bp deleted → frameshift`
244.2	`CATGGTGAGCTGCGAGAATAGCCGGGCGC------------------CCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`18 bp deleted → no frameshift`
242.8	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCC----------CCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`10 bp deleted → frameshift`
233.7	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAG-----GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`5 bp deleted → frameshift`
230.8	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCC-----------CGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`11 bp deleted → frameshift`
210.0	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCC---------------------ACTTCCGGCGCGCCGAGTCC`	`21 bp deleted → no frameshift`
199.8	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGC----------------------CCTGGCCACTTCCGGCGCGCCGAGTCC`	`22 bp deleted → frameshift`
182.1	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGT----------CGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`10 bp deleted → frameshift`
180.6	`CATGGTGAGCTGCGAGAATAGCCGGGCGC------------------------CCTGGCCACTTCCGGCGCGCCGAGTCC`	`24 bp deleted → no frameshift`
179.6	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCC----------------TGGCCACTTCCGGCGCGCCGAGTCC`	`16 bp deleted → frameshift`
172.2	`CATGGTGAGCTGCGAGAATAGCC-------------------------CCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`25 bp deleted → frameshift`
170.8	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGC------------------------------------------CGAGTCC`	`42 bp deleted → no frameshift`
170.8	`CATGGTGAGCTGCGAGAATAGCCGGG-----------------TCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`17 bp deleted → frameshift`
162.8	`CATGGTGAGCTGCGAGAATAGCCGG------------------TCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`18 bp deleted → no frameshift`
155.4	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCG---------------------------GCGCGCCGAGTCC`	`27 bp deleted → no frameshift`
150.5	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTG------------------------GCCACTTCCGGCGCGCCGAGTCC`	`24 bp deleted → no frameshift`
148.2	`CATGGTGAGCTGCGAGAATAGCCG----------------------------CCCTGGCCACTTCCGGCGCGCCGAGTCC`	`28 bp deleted → frameshift`
147.2	`CATGGTGAGCTGCGAGAATAGCCGGGC--------------------CCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`20 bp deleted → frameshift`
139.2	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCG-----------------------------------AGTCC`	`35 bp deleted → frameshift`
133.2	`CATGGTGAGCTGCGAGAATAGCCG----------------------CCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`22 bp deleted → frameshift`
127.2	`CATGGTGAGCTGCGAGAATAGCC-------------------------------CTGGCCACTTCCGGCGCGCCGAGTCC`	`31 bp deleted → frameshift`
121.2	`CATGGTGAGCTGCGAGAATAGCCGGGC--------------------------------CACTTCCGGCGCGCCGAGTCC`	`32 bp deleted → frameshift`
109.2	`CATGGTGAGCTGCGAGAATAGCCGGGC--------------------------CCTGGCCACTTCCGGCGCGCCGAGTCC`	`26 bp deleted → frameshift`
109.2	`CATGGTGAGCTGCGAGAATAGCC--------------------------CCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`26 bp deleted → frameshift`
108.0	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGC----------------------------------------GCCGAGTCC`	`40 bp deleted → frameshift`
105.0	`CATGGTGAGCTGCGAGAATAG---------------------GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`21 bp deleted → no frameshift`
103.6	`CATGGTGAGCTGCGAGAATAGCC---------------------------CGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`27 bp deleted → no frameshift`
99.9	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTG----------------------GCCACTTCCGGCGCGCCGAGTCC`	`22 bp deleted → frameshift`
98.8	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGC----------------------------CACTTCCGGCGCGCCGAGTCC`	`28 bp deleted → frameshift`
94.2	`CATGGTGAGCTGCGAGAATAGCC-------------------------------------ACTTCCGGCGCGCCGAGTCC`	`37 bp deleted → frameshift`
92.8	`CATGGTGAGCTGCGAGAATAGCCGG-------------------------------------------CGCGCCGAGTCC`	`43 bp deleted → frameshift`
89.2	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCG------------------------------CGCCGAGTCC`	`30 bp deleted → no frameshift`
89.2	`CATGGTGAGCTGCGAGAATAGCCGGGCGC------------------------------CACTTCCGGCGCGCCGAGTCC`	`30 bp deleted → no frameshift`
88.8	`CATGGTGAGCTGCGAGAATAGCCGGGCGC--------------------------------------------CGAGTCC`	`44 bp deleted → frameshift`
84.8	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGC-------------------------------GCGCCGAGTCC`	`31 bp deleted → frameshift`
81.9	`CATGGTGAGCTGCGAG--------------------------GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`26 bp deleted → frameshift`
80.8	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCG--------------------------------CCGAGTCC`	`32 bp deleted → frameshift`
80.8	`CATGGTGAGCTGCGAGAATAGCC--------------------------------TGGCCACTTCCGGCGCGCCGAGTCC`	`32 bp deleted → frameshift`
80.8	`CATGGTGAGCTGCG--------------------------------CCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`32 bp deleted → frameshift`
76.8	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGC---------------------------------GCCGAGTCC`	`33 bp deleted → no frameshift`
76.8	`CATGGTGAGCTGCGAGAATAGCCGG---------------------------------CCACTTCCGGCGCGCCGAGTCC`	`33 bp deleted → no frameshift`
75.0	`CATGGT--------------------------------------CGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`38 bp deleted → frameshift`
73.2	`CATGGTGAGCTGC----------------------------------CCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`34 bp deleted → frameshift`
67.5	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAG----------------------------------------TCC`	`40 bp deleted → frameshift`
63.6	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCT-------------------------------TCCGGCGCGCCGAGTCC`	`31 bp deleted → frameshift`
62.8	`CATGGTGAGCTGCGAGAATAGCCGGGCGCG-------------------------------------GCGCGCCGAGTCC`	`37 bp deleted → frameshift`
62.8	`CATGGTGAGC-------------------------------------CCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`37 bp deleted → frameshift`
62.4	`CATGGTGAGCTGCGAGAATAGCCG---------------------------------------------------AGTCC`	`51 bp deleted → no frameshift`
60.0	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGC--------------------------------------GCGCCGAGTCC`	`38 bp deleted → frameshift`
60.0	`CATGGTGAGCTGCG--------------------------------------CCCTGGCCACTTCCGGCGCGCCGAGTCC`	`38 bp deleted → frameshift`
57.6	`CATGGTGAG---------------------------------GTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCC`	`33 bp deleted → no frameshift`
56.8	`CATGGTGAGCTGCGAGAATAGCCGGGCG---------------------------------------GCGCGCCGAGTCC`	`39 bp deleted → no frameshift`
54.0	`CATGGTGAGCTGC----------------------------------------CCTGGCCACTTCCGGCGCGCCGAGTCC`	`40 bp deleted → frameshift`
52.5	`CATGGTGAGCTG---------------------------------------------GCCACTTCCGGCGCGCCGAGTCC`	`45 bp deleted → no frameshift`
51.6	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCC-----------------------------------------`	`41 bp deleted → frameshift`
46.4	`CATGGTGAGC-------------------------------------------CCTGGCCACTTCCGGCGCGCCGAGTCC`	`43 bp deleted → frameshift`
42.0	`CATGGTGAGCTGCGAGAATAGCCGGGCGCG---------------------------------------------AGTCC`	`45 bp deleted → no frameshift`
40.0	`CATGGTGAGCTGCGAGAATAGCCGGGC----------------------------------------------CGAGTCC`	`46 bp deleted → frameshift`
40.0	`CATGGTGAGCTGCGAGAATAGCCG----------------------------------------------CGCCGAGTCC`	`46 bp deleted → frameshift`
40.0	`CATGGTGAGCTGC----------------------------------------------CACTTCCGGCGCGCCGAGTCC`	`46 bp deleted → frameshift`
38.0	`CATGGTGAGCTGCGAGAATAGCCGGGCG-----------------------------------------------AGTCC`	`47 bp deleted → frameshift`
38.0	`CATGGTGAGCTGCGAGAATAGC-----------------------------------------------GCGCCGAGTCC`	`47 bp deleted → frameshift`
36.6	`CATGGTGAGCTGCG--------------------------------------------------------CGCCGAGTCC`	`56 bp deleted → frameshift`
36.4	`CATGGTGAGCTGCGAGAATAGCCG------------------------------------------------CCGAGTCC`	`48 bp deleted → no frameshift`
35.5	`CATGG-----------------------------------------------------CCACTTCCGGCGCGCCGAGTCC`	`53 bp deleted → frameshift`
34.4	`CATGGTGAGCTGCGAGAATAGC-------------------------------------------------GCCGAGTCC`	`49 bp deleted → frameshift`
34.4	`CATGGTGAGC-------------------------------------------------CACTTCCGGCGCGCCGAGTCC`	`49 bp deleted → frameshift`
33.3	`CATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGT--------------------------------------------CC`	`44 bp deleted → frameshift`
33.0	`CATGGTGAGCTGCG----------------------------------------------------------CCGAGTCC`	`58 bp deleted → frameshift`
32.9	`CATGGTGAGCTGCGAG-------------------------------------------------------------TCC`	`61 bp deleted → frameshift`
28.4	`CATGGTGAGCTGCG-----------------------------------------------------GCGCGCCGAGTCC`	`53 bp deleted → frameshift`
24.6	`CATGGTG--------------------------------------------------GCCACTTCCGGCGCGCCGAGTCC`	`50 bp deleted → frameshift`
23.2	`CATGGTGAGCTGCGAGAATAGCC---------------------------------------------------------`	`57 bp deleted → no frameshift`
22.2	`CATGGTGAGCT----------------------------------------------------TCCGGCGCGCCGAGTCC`	`52 bp deleted → frameshift`
20.8	`CATGGTGAGC-----------------------------------------------------------GCGCCGAGTCC`	`59 bp deleted → frameshift`
20.0	`CATGGTGAGCTGC------------------------------------------------------------CGAGTCC`	`60 bp deleted → no frameshift`
18.8	`CATGGTGAGC-------------------------------------------------------------GCCGAGTCC`	`61 bp deleted → frameshift`
18.3	`CATGGTGAGCTGCGAGAATAG--------------------------------------------------------TCC`	`56 bp deleted → frameshift`
17.2	`CATGGTGAGC---------------------------------------------------------------CGAGTCC`	`63 bp deleted → no frameshift`
17.2	`CATGG---------------------------------------------------------------CGCGCCGAGTCC`	`63 bp deleted → no frameshift`
16.5	`CATGGTGAG--------------------------------------------------------------------TCC`	`68 bp deleted → frameshift`
15.6	`CATGGTGAGCTGCGAGA-----------------------------------------------------------GTCC`	`59 bp deleted → frameshift`
15.6	`CA-----------------------------------------------------------CTTCCGGCGCGCCGAGTCC`	`59 bp deleted → frameshift`
8.1	`CATGGT------------------------------------------------------------------------CC`	`72 bp deleted → no frameshift`

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