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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529590_F | TCGTCGGCAGCGTCCACGATGGAGGGGAAGACG | 59.8 | 1.00 |
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529590_R | GTCTCGTGGGCTCGGGCTATTCTCGCAGCTCACCA | 60.1 | 1.00 |
| mm0__chr22 41.08 Mbp_chr22_41075172_F | TCGTCGGCAGCGTCATGCCCACCATCATGACCTG | 60.1 | 1.00 |
| mm0__chr22 41.08 Mbp_chr22_41075172_R | GTCTCGTGGGCTCGGTATTGCTGCGCTCGTTGTTG | 59.8 | 1.00 |
| mm2__chr5 77.78 Mbp_chr5_77784930_F | TCGTCGGCAGCGTCATATCACCGCGCTCGTCATT | 59.9 | 0.70 |
| mm2__chr5 77.78 Mbp_chr5_77784930_R | GTCTCGTGGGCTCGGAACGATGGAGGGGAAGACTG | 59.0 | 0.70 |
| mm4__chr2 81.59 Mbp_chr2_81590143_F | Primer3: not found at this Tm | N.d. | 0.46 |
| mm4__chr2 81.59 Mbp_chr2_81590143_R | Primer3: not found at this Tm | N.d. | 0.46 |
| mm1__chr2 131.63 Mbp_chr2_131626182_F | TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG | 59.8 | 0.46 |
| mm1__chr2 131.63 Mbp_chr2_131626182_R | GTCTCGTGGGCTCGGACTCCTTCTGATGCATGCCC | 60.1 | 0.46 |
| mm1__chr2 131.26 Mbp_chr2_131263599_F | TCGTCGGCAGCGTCTGGATGATGATACCGCCGTG | 59.9 | 0.46 |
| mm1__chr2 131.26 Mbp_chr2_131263599_R | GTCTCGTGGGCTCGGGATGGAAGGGAAGACAGCCC | 60.1 | 0.46 |
| mm1__chr2 130.66 Mbp_chr2_130656793_F | TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG | 59.8 | 0.46 |
| mm1__chr2 130.66 Mbp_chr2_130656793_R | GTCTCGTGGGCTCGGGATGGAAGGGAAGACAGCCC | 60.1 | 0.46 |
| mm1__chr2 130.46 Mbp_chr2_130463876_F | TCGTCGGCAGCGTCGATGGAAGGGAAGACAGCCC | 60.1 | 0.46 |
| mm1__chr2 130.46 Mbp_chr2_130463876_R | GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG | 59.8 | 0.46 |
| mm1__chr2 130.08 Mbp_chr2_130075304_F | TCGTCGGCAGCGTCGATGGAAGGGAAGACAGCCC | 60.1 | 0.46 |
| mm1__chr2 130.08 Mbp_chr2_130075304_R | GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG | 59.8 | 0.46 |
| mm4__chr2 74.61 Mbp_chr2_74606328_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4__chr2 74.61 Mbp_chr2_74606328_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm3__chr19 9.52 Mbp_chr19_9518193_F | TCGTCGGCAGCGTCGCTGTCAGCTCACCATGGAT | 60.1 | 0.38 |
| mm3__chr19 9.52 Mbp_chr19_9518193_R | GTCTCGTGGGCTCGGCCACGATGGAGGGGAAGATG | 59.8 | 0.38 |
| mm2__chr1 22.79 Mbp_chr1_22785026_F | TCGTCGGCAGCGTCCCAATGCGGAAGAGGTGGAT | 60.1 | 0.35 |
| mm2__chr1 22.79 Mbp_chr1_22785026_R | GTCTCGTGGGCTCGGCAGACGGTGCCATTCTCAAC | 59.2 | 0.35 |
| mm4__chr20 50.25 Mbp_chr20_50248458_F | TCGTCGGCAGCGTCCATCCTTGGGCCTGTGACC | 60.3 | 0.34 |
| mm4__chr20 50.25 Mbp_chr20_50248458_R | GTCTCGTGGGCTCGGGAAGCTATTGCCTGGGACGA | 59.8 | 0.34 |
| mm4__chr1 22.58 Mbp_chr1_22576836_F | TCGTCGGCAGCGTCACCCAAGATGTACTGCAGCG | 60.3 | 0.28 |
| mm4__chr1 22.58 Mbp_chr1_22576836_R | GTCTCGTGGGCTCGGCTCACCCACACAGGCATCC | 60.3 | 0.28 |
| mm4__chr2 47.24 Mbp_chr2_47243478_F | Primer3: not found at this Tm | N.d. | 0.26 |
| mm4__chr2 47.24 Mbp_chr2_47243478_R | Primer3: not found at this Tm | N.d. | 0.26 |
| mm1__chr5 98.34 Mbp_chr5_98338787_F | TCGTCGGCAGCGTCGCTCCTCATCAACAAGGGCT | 60.0 | 0.24 |
| mm1__chr5 98.34 Mbp_chr5_98338787_R | GTCTCGTGGGCTCGGTTTTGACCCATGCCCACCAT | 60.1 | 0.24 |
| mm4__chr5 156.90 Mbp_chr5_156896225_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4__chr5 156.90 Mbp_chr5_156896225_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4__chr14 102.46 Mbp_chr14_102461655_F | TCGTCGGCAGCGTCAGCAAGCCCCAGATATCAGC | 59.8 | 0.22 |
| mm4__chr14 102.46 Mbp_chr14_102461655_R | GTCTCGTGGGCTCGGCAAAGCAGGCAGGATCAGGA | 60.0 | 0.22 |
| mm4__chr4 16.64 Mbp_chr4_16637495_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr4 16.64 Mbp_chr4_16637495_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr9 133.25 Mbp_chr9_133252122_F | TCGTCGGCAGCGTCAGAGGGGCAAGGAAGCAAAA | 59.8 | 0.22 |
| mm4__chr9 133.25 Mbp_chr9_133252122_R | GTCTCGTGGGCTCGGGCAGCCCCAAAGTGTCATCT | 60.6 | 0.22 |
| mm4__chr14 99.98 Mbp_chr14_99980422_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4__chr14 99.98 Mbp_chr14_99980422_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm3__chr10 78.74 Mbp_chr10_78739613_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm3__chr10 78.74 Mbp_chr10_78739613_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4__chr6 11.32 Mbp_chr6_11324450_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4__chr6 11.32 Mbp_chr6_11324450_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4__chr19 58.56 Mbp_chr19_58563366_F | TCGTCGGCAGCGTCGGGTCCTGTTCACTCCTCAG | 59.3 | 0.19 |
| mm4__chr19 58.56 Mbp_chr19_58563366_R | GTCTCGTGGGCTCGGGTGTAAGCCCTCACCTCCAC | 60.0 | 0.19 |
| mm3__chrX 46.29 Mbp_chrX_46289061_F | TCGTCGGCAGCGTCTGGAGGGGAAGACAACTGGG | 61.1 | 0.17 |
| mm3__chrX 46.29 Mbp_chrX_46289061_R | GTCTCGTGGGCTCGGCTCCATCACCAGTCCACACC | 60.0 | 0.17 |
| mm2__chr1 223.86 Mbp_chr1_223864784_F | TCGTCGGCAGCGTCTGACCCATGCTCACCATCAC | 60.0 | 0.17 |
| mm2__chr1 223.86 Mbp_chr1_223864784_R | GTCTCGTGGGCTCGGCGCTCGTCATTGACAACAGC | 60.1 | 0.17 |
| mm4__chr9 131.07 Mbp_chr9_131069729_F | TCGTCGGCAGCGTCCTCTAAACCCAGCACGCACT | 60.3 | 0.16 |
| mm4__chr9 131.07 Mbp_chr9_131069729_R | GTCTCGTGGGCTCGGGCAGCGGTCACATTTGTAGC | 60.1 | 0.16 |
| mm4__chr6 5.57 Mbp_chr6_5566989_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4__chr6 5.57 Mbp_chr6_5566989_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4__chr5 67.02 Mbp_chr5_67019260_F | TCGTCGGCAGCGTCTCCTGGGGCACATTGGAAAG | 60.2 | 0.15 |
| mm4__chr5 67.02 Mbp_chr5_67019260_R | GTCTCGTGGGCTCGGAGTGTCAATGGGCCCATCTC | 59.7 | 0.15 |
| mm4__chr2 97.10 Mbp_chr2_97095824_F | TCGTCGGCAGCGTCGGATGACAGGAGGTGGAAGTG | 60.0 | 0.15 |
| mm4__chr2 97.10 Mbp_chr2_97095824_R | GTCTCGTGGGCTCGGAAGCCCTGTCTCCATGAACG | 60.0 | 0.15 |
| mm4__chr9 86.68 Mbp_chr9_86680530_F | TCGTCGGCAGCGTCAGACCATGTGAATAGGAAGGCA | 59.1 | 0.15 |
| mm4__chr9 86.68 Mbp_chr9_86680530_R | GTCTCGTGGGCTCGGACCAAACGCAAATCAATGCA | 57.4 | 0.15 |
| mm4__chr16 80.04 Mbp_chr16_80041093_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr16 80.04 Mbp_chr16_80041093_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr15 73.27 Mbp_chr15_73265533_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr15 73.27 Mbp_chr15_73265533_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm2__chr18 62.44 Mbp_chr18_62442052_F | TCGTCGGCAGCGTCCCTCGTCATCGACAATGGCT | 60.1 | 0.12 |
| mm2__chr18 62.44 Mbp_chr18_62442052_R | GTCTCGTGGGCTCGGGAGTCCTTCTGACCCATGCC | 60.1 | 0.12 |
| mm4__chr11 46.40 Mbp_chr11_46397259_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr11 46.40 Mbp_chr11_46397259_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm1__chr9 100.73 Mbp_chr9_100731283_F | TCGTCGGCAGCGTCGCCAGCTCACCATGGATGAT | 60.1 | 0.12 |
| mm1__chr9 100.73 Mbp_chr9_100731283_R | GTCTCGTGGGCTCGGCTAGGGAGCTCCACGATGGA | 60.4 | 0.12 |
| mm1__chr22 15.72 Mbp_chr22_15721530_F | TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG | 59.8 | 0.12 |
| mm1__chr22 15.72 Mbp_chr22_15721530_R | GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG | 60.0 | 0.12 |
| mm1__chr14 19.40 Mbp_chr14_19402841_F | TCGTCGGCAGCGTCATCATGTTCTGGTGCCTGGG | 60.0 | 0.12 |
| mm1__chr14 19.40 Mbp_chr14_19402841_R | GTCTCGTGGGCTCGGGTGCTCGTCATTGACAACGG | 59.8 | 0.12 |
| mm1__chr14 19.00 Mbp_chr14_18998911_F | TCGTCGGCAGCGTCGTGCTCGTCATTGACAACGG | 59.8 | 0.12 |
| mm1__chr14 19.00 Mbp_chr14_18998911_R | GTCTCGTGGGCTCGGATCATGTTCTGGTGCCTGGG | 60.0 | 0.12 |
| mm4__chr11 133.55 Mbp_chr11_133554323_F | TCGTCGGCAGCGTCTAGGCTGTCAACGTCCAAGC | 60.3 | 0.11 |
| mm4__chr11 133.55 Mbp_chr11_133554323_R | GTCTCGTGGGCTCGGCTAGCGCTATTCGTTCCCGG | 60.6 | 0.11 |
| mm4__chr2 172.63 Mbp_chr2_172633621_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr2 172.63 Mbp_chr2_172633621_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr7 2.56 Mbp_chr7_2559194_F | TCGTCGGCAGCGTCCGAGCCAGCAACCCTGAG | 60.4 | 0.10 |
| mm4__chr7 2.56 Mbp_chr7_2559194_R | GTCTCGTGGGCTCGGCCCCTCGCCCGTTCTTAC | 59.8 | 0.10 |
| mm4__chr22 50.05 Mbp_chr22_50045601_F | TCGTCGGCAGCGTCCTCCCTGCAGTGTCTCTGTG | 60.0 | 0.10 |
| mm4__chr22 50.05 Mbp_chr22_50045601_R | GTCTCGTGGGCTCGGGTGTGGTTCTGTGCTAGGCT | 59.9 | 0.10 |
| mm4__chr11 64.62 Mbp_chr11_64617873_F | TCGTCGGCAGCGTCCAGTGGTGCAAATGTGGACG | 60.0 | 0.09 |
| mm4__chr11 64.62 Mbp_chr11_64617873_R | GTCTCGTGGGCTCGGAGAGGAAACTGCCAGAAGCC | 59.9 | 0.09 |
| mm4__chr22 21.92 Mbp_chr22_21918643_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr22 21.92 Mbp_chr22_21918643_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr7 6.61 Mbp_chr7_6607554_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr7 6.61 Mbp_chr7_6607554_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr11 60.92 Mbp_chr11_60921930_F | TCGTCGGCAGCGTCCGTCTACTGGCTGCTGTCTC | 60.1 | 0.09 |
| mm4__chr11 60.92 Mbp_chr11_60921930_R | GTCTCGTGGGCTCGGCAGGCACCGACCTCATCAG | 60.1 | 0.09 |
| mm2__chr9 6.83 Mbp_chr9_6834499_F | TCGTCGGCAGCGTCCCGCTACCAGCTCACCATAG | 59.9 | 0.08 |
| mm2__chr9 6.83 Mbp_chr9_6834499_R | GTCTCGTGGGCTCGGACACGATGAAGGGGAAGACG | 59.7 | 0.08 |
| mm4__chr9 137.16 Mbp_chr9_137160287_F | TCGTCGGCAGCGTCGATGAAGGGAGGCACGGG | 59.8 | 0.08 |
| mm4__chr9 137.16 Mbp_chr9_137160287_R | GTCTCGTGGGCTCGGTGAGAAACAGCCCAGCCTG | 59.9 | 0.08 |
| mm4__chr14 100.82 Mbp_chr14_100824536_F | TCGTCGGCAGCGTCTTCTTGACGTCTGTGGCTGG | 60.2 | 0.08 |
| mm4__chr14 100.82 Mbp_chr14_100824536_R | GTCTCGTGGGCTCGGCCCCTTTACAGCCTGTGGAG | 60.0 | 0.08 |
| mm4__chr22 30.71 Mbp_chr22_30712840_F | TCGTCGGCAGCGTCACAGGTTGTATCTTGGGGTCA | 58.6 | 0.08 |
| mm4__chr22 30.71 Mbp_chr22_30712840_R | GTCTCGTGGGCTCGGACCAGGGCTCCTTCCTTTAC | 59.0 | 0.08 |
| mm4__chr16 27.77 Mbp_chr16_27774151_F | TCGTCGGCAGCGTCGCCCACCACAGTTAGGGAAA | 59.8 | 0.08 |
| mm4__chr16 27.77 Mbp_chr16_27774151_R | GTCTCGTGGGCTCGGCACCAGGGGAAGGCAGAAAA | 60.1 | 0.08 |
| mm2__chr2 73.90 Mbp_chr2_73901358_F | TCGTCGGCAGCGTCGTGTGAAGAGGAGACCACCG | 60.0 | 0.07 |
| mm2__chr2 73.90 Mbp_chr2_73901358_R | GTCTCGTGGGCTCGGCCCACAATGGAGGGGAAGAC | 60.0 | 0.07 |
| mm3__chr10 110.51 Mbp_chr10_110508586_F | TCGTCGGCAGCGTCTTGCTTGCCCTGCTAATGGA | 59.9 | 0.07 |
| mm3__chr10 110.51 Mbp_chr10_110508586_R | GTCTCGTGGGCTCGGGTATTGCCTCTGGAGCCCAC | 60.4 | 0.07 |
| mm4__chr10 119.44 Mbp_chr10_119436742_F | TCGTCGGCAGCGTCCGAGACCAAGGATGCACTGT | 60.0 | 0.07 |
| mm4__chr10 119.44 Mbp_chr10_119436742_R | GTCTCGTGGGCTCGGGCATAAAACAAGGCCCGCTC | 60.1 | 0.07 |
| mm4__chrX 137.15 Mbp_chrX_137147230_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrX 137.15 Mbp_chrX_137147230_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr5 131.66 Mbp_chr5_131660048_F | TCGTCGGCAGCGTCATGCCCATCATCACACCCTG | 60.1 | 0.06 |
| mm4__chr5 131.66 Mbp_chr5_131660048_R | GTCTCGTGGGCTCGGTGTCGTCGACAATGGCTCTG | 60.3 | 0.06 |
| mm4__chr19 18.79 Mbp_chr19_18788710_F | TCGTCGGCAGCGTCAGTCTGCATGCTCGTGGC | 60.4 | 0.06 |
| mm4__chr19 18.79 Mbp_chr19_18788710_R | GTCTCGTGGGCTCGGCAGGGCTCCTTCCAGTGC | 60.0 | 0.06 |
| mm4__chr15 77.57 Mbp_chr15_77572058_F | TCGTCGGCAGCGTCATCGTGGTGTCACTTGGCAT | 59.9 | 0.06 |
| mm4__chr15 77.57 Mbp_chr15_77572058_R | GTCTCGTGGGCTCGGTCTCTGGCCATTCTCCTTGC | 59.7 | 0.06 |
| mm4__chr7 44.31 Mbp_chr7_44308785_F | TCGTCGGCAGCGTCCATGAGGCACAGAGAAGGCA | 60.0 | 0.05 |
| mm4__chr7 44.31 Mbp_chr7_44308785_R | GTCTCGTGGGCTCGGCCCCTGGTCTCTTGTGAGGA | 60.5 | 0.05 |
| mm4__chr1 40.65 Mbp_chr1_40649154_F | TCGTCGGCAGCGTCGGCTCCTCTCCTAGTTGTGC | 59.8 | 0.05 |
| mm4__chr1 40.65 Mbp_chr1_40649154_R | GTCTCGTGGGCTCGGGTGCCCCATGTCTCTTCCTG | 60.3 | 0.05 |
| mm4__chr3 189.93 Mbp_chr3_189934523_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr3 189.93 Mbp_chr3_189934523_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3__chr5 124.13 Mbp_chr5_124129103_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3__chr5 124.13 Mbp_chr5_124129103_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr2 120.87 Mbp_chr2_120874522_F | TCGTCGGCAGCGTCGCGTCAGCATTTGGGGAAAG | 60.1 | 0.04 |
| mm4__chr2 120.87 Mbp_chr2_120874522_R | GTCTCGTGGGCTCGGCGGATGATAGATGCCACCCG | 60.3 | 0.04 |
| mm4__chr3 122.70 Mbp_chr3_122696231_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr3 122.70 Mbp_chr3_122696231_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr2 102.48 Mbp_chr2_102479311_F | TCGTCGGCAGCGTCCGTGAACGAGCAGCTCTACA | 60.1 | 0.04 |
| mm4__chr2 102.48 Mbp_chr2_102479311_R | GTCTCGTGGGCTCGGCACCCCGAACCCTCATCTC | 59.4 | 0.04 |
| mm4__chr2 91.59 Mbp_chr2_91588366_F | TCGTCGGCAGCGTCCAGACACAGTCTCCTTCCGG | 59.7 | 0.04 |
| mm4__chr2 91.59 Mbp_chr2_91588366_R | GTCTCGTGGGCTCGGGCAGAGGAGCTGGAATGAGG | 60.1 | 0.04 |
| mm4__chr1_KI270712v1_random 131.35 Kbp_chr1_KI270712v1_random_131352_F | TCGTCGGCAGCGTCACTTAATGGCACCCGAGCAG | 60.3 | 0.04 |
| mm4__chr1_KI270712v1_random 131.35 Kbp_chr1_KI270712v1_random_131352_R | GTCTCGTGGGCTCGGCATCTCCACCAGCCCCTTC | 59.7 | 0.04 |
| mm4__chr1 144.79 Mbp_chr1_144787685_F | TCGTCGGCAGCGTCCATCTCCACCAGCCCCTTC | 59.7 | 0.04 |
| mm4__chr1 144.79 Mbp_chr1_144787685_R | GTCTCGTGGGCTCGGACTTAATGGCACCCGAGCAG | 60.3 | 0.04 |
| mm4__chr1 144.18 Mbp_chr1_144178679_F | TCGTCGGCAGCGTCACTTAATGGCACCCGAGCAG | 60.3 | 0.04 |
| mm4__chr1 144.18 Mbp_chr1_144178679_R | GTCTCGTGGGCTCGGCATCTCCACCAGCCCCTTC | 59.7 | 0.04 |
| mm4__chr1 143.35 Mbp_chr1_143353094_F | TCGTCGGCAGCGTCACTTAATGGCACCCGAGCAG | 60.3 | 0.04 |
| mm4__chr1 143.35 Mbp_chr1_143353094_R | GTCTCGTGGGCTCGGCATCTCCACCAGCCCCTTC | 59.7 | 0.04 |
| mm4__chr1 18.09 Mbp_chr1_18092188_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr1 18.09 Mbp_chr1_18092188_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chrY 13.49 Mbp_chrY_13487599_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chrY 13.49 Mbp_chrY_13487599_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr10 66.45 Mbp_chr10_66452448_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr10 66.45 Mbp_chr10_66452448_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr6 105.54 Mbp_chr6_105538658_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr6 105.54 Mbp_chr6_105538658_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr1 1.33 Mbp_chr1_1327653_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr1 1.33 Mbp_chr1_1327653_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr19 787.64 Kbp_chr19_787639_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr19 787.64 Kbp_chr19_787639_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr15 89.22 Mbp_chr15_89221817_F | TCGTCGGCAGCGTCAAAGCCCTCCTTTGTGTGCT | 60.1 | 0.03 |
| mm4__chr15 89.22 Mbp_chr15_89221817_R | GTCTCGTGGGCTCGGAAAGAGGGAATGGGACTGGC | 59.6 | 0.03 |
| mm4__chr12 24.18 Mbp_chr12_24181637_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr12 24.18 Mbp_chr12_24181637_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr22 49.01 Mbp_chr22_49009568_F | TCGTCGGCAGCGTCAGTCTCCCCTGCTTCCTTCT | 59.8 | 0.03 |
| mm4__chr22 49.01 Mbp_chr22_49009568_R | GTCTCGTGGGCTCGGAATTCACCAGTCCCTTGCCG | 60.6 | 0.03 |
| mm4__chr11 61.85 Mbp_chr11_61845422_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr11 61.85 Mbp_chr11_61845422_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr9 127.22 Mbp_chr9_127219504_F | TCGTCGGCAGCGTCCAAGGGTCCCTCGCATTCTT | 60.0 | 0.02 |
| mm4__chr9 127.22 Mbp_chr9_127219504_R | GTCTCGTGGGCTCGGGTCCTGTCTCAGAAGGGCTG | 59.7 | 0.02 |
| mm4__chr19 11.25 Mbp_chr19_11254971_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr19 11.25 Mbp_chr19_11254971_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr15 94.23 Mbp_chr15_94231642_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr15 94.23 Mbp_chr15_94231642_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr9 38.35 Mbp_chr9_38354283_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr9 38.35 Mbp_chr9_38354283_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr16 14.37 Mbp_chr16_14372616_F | TCGTCGGCAGCGTCCAGCAGCCCCACACAAAG | 58.9 | 0.02 |
| mm4__chr16 14.37 Mbp_chr16_14372616_R | GTCTCGTGGGCTCGGAAGTGGAGCTGCCTGCAG | 59.9 | 0.02 |
| mm4__chr4 8.77 Mbp_chr4_8773760_F | TCGTCGGCAGCGTCCGAGCCCTGCAGATGAAGG | 60.5 | 0.02 |
| mm4__chr4 8.77 Mbp_chr4_8773760_R | GTCTCGTGGGCTCGGCACACGTTACCCAGAGGCAC | 60.9 | 0.02 |
| mm4__chr17 73.44 Mbp_chr17_73436200_F | TCGTCGGCAGCGTCGTCGAAGAGCACGTAGAGGG | 59.9 | 0.01 |
| mm4__chr17 73.44 Mbp_chr17_73436200_R | GTCTCGTGGGCTCGGAACAATGTGCCAGGCTGAGA | 59.8 | 0.01 |
| mm4__chr10 132.27 Mbp_chr10_132270377_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr10 132.27 Mbp_chr10_132270377_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chrX 85.56 Mbp_chrX_85559594_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chrX 85.56 Mbp_chrX_85559594_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr10 133.48 Mbp_chr10_133475298_F | TCGTCGGCAGCGTCAGCCACACTCCTCACTCAGT | 60.4 | 0.01 |
| mm4__chr10 133.48 Mbp_chr10_133475298_R | GTCTCGTGGGCTCGGAGGTCAGGGCAGGGTAGAAT | 59.9 | 0.01 |
| mm4__chr12 122.97 Mbp_chr12_122974134_F | TCGTCGGCAGCGTCCAAACTGCTGCAAACGGAGA | 59.3 | 0.01 |
| mm4__chr12 122.97 Mbp_chr12_122974134_R | GTCTCGTGGGCTCGGTCATTACCCGCCTAACTGCC | 59.8 | 0.01 |
| mm4__chr7 5.39 Mbp_chr7_5388955_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr7 5.39 Mbp_chr7_5388955_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr10 15.30 Mbp_chr10_15295741_F | TCGTCGGCAGCGTCGCATTTTATGGGCTGGCCAC | 60.1 | 0.00 |
| mm4__chr10 15.30 Mbp_chr10_15295741_R | GTCTCGTGGGCTCGGCAGGAGTGCTGGGCTACTTG | 60.3 | 0.00 |
| mm4__chr11 17.25 Mbp_chr11_17249184_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr11 17.25 Mbp_chr11_17249184_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr2 240.72 Mbp_chr2_240722360_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr2 240.72 Mbp_chr2_240722360_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chrY 988.13 Kbp_chrY_988131_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chrY 988.13 Kbp_chrY_988131_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 36.15 Mbp_chr5_36153974_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 36.15 Mbp_chr5_36153974_R | Primer3: not found at this Tm | N.d. | 0.00 |
Primers underlined, off-targets in bold.
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529590 |
CACGATGGAGGGGAAGACGGCCCGGGGGGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGAGCCGTTGTCGACGA CGAGCGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGC |
| mm0__chr22 41.08 Mbp_chr22_41075172 |
ATGCCCACCATCATGACCTGGTGCCTGGTGCACCCCACGATGGAAGGGAAGATGGCCCAGGGGACATCGTCACCCGCGAA GCCGGCCTTGCACATGCCAGAGCTGTTGTCAACAACGAGCGCAGCAATA |
| mm2__chr5 77.78 Mbp_chr5_77784930 |
ATATCACCGCGCTCGTCATTGACAACGGCTCCGGCATGTGCAAGGCCAGCTTCACGGGCGACAATGCCGCCCGGGCAGTC TTCCCCTCCATCGTT |
| mm1__chr2 131.63 Mbp_chr2_131626182 |
GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCAGGCTGTCTTCCCTTC TATCGTGGGGCGCCCCAGGCACCAGGGCATGATGGAGGGCATGCATCAGAAGGAGT |
| mm1__chr2 131.26 Mbp_chr2_131263599 |
TGGATGATGATACCGCCGTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCC CGGGCTGTCTTCCCTTCCATC |
| mm1__chr2 130.66 Mbp_chr2_130656793 |
GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCGGGCGACGATGCCCCCCGGGCTGTCTTCCCTTC CATC |
| mm1__chr2 130.46 Mbp_chr2_130463876 |
GATGGAAGGGAAGACAGCCCGGGGGGCATCGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGA GCAC |
| mm1__chr2 130.08 Mbp_chr2_130075304 |
GATGGAAGGGAAGACAGCCCGGGGGGCATCGTCGCCCGCAAAGCCGGCCTTGCACATGCCAGAGCCGTTGTCAATGACGA GCAC |
| mm3__chr19 9.52 Mbp_chr19_9518193 |
GCTGTCAGCTCACCATGGATGATGATATTGAGGTGCTTGTAGTTAACAATGGCTCCGGCATGTGCAAGGTCAACTTCGTG GGCGACAATGCCCCCCGGGCCATCTTCCCCTCCATCGTGG |
| mm2__chr1 22.79 Mbp_chr1_22785026 |
CCAATGCGGAAGAGGTGGATGTACCCATCAAGCTCTACTGTAACGGGGACGGCGAGTGGCTGGTGCCCATCGGGCGCTGC ATGTGCAAAGCAGGCTTCGAGGCCGTTGAGAATGGCACCGTCTG |
| mm4__chr20 50.25 Mbp_chr20_50248458 |
CATCCTTGGGCCTGTGACCCAGGCCCCTGTGCCACTTGGCATCCACGAAGCTGGCATTGCACTGGACTTGGGACTGGCTG GGTTTTCCCCTTTCTTCGTCCCAGGCAATAGCTTC |
| mm4__chr1 22.58 Mbp_chr1_22576836 |
ACCCAAGATGTACTGCAGCGCGGAGGGCGAGTGGCTCGTGCCCATCGGCAAATGCGTGTGCAGTGCCGGCTACGAGGAGC GGCGGGATGCCTGTGTGGGTGAG |
| mm1__chr5 98.34 Mbp_chr5_98338787 |
GCTCCTCATCAACAAGGGCTCTGGCATGTGCAAGGCCGGCTTCACAGGTGACAATGCCCCCGGGCCATCTTCCCCTCTGT CATGGGTGCCAGGGAGTGATGGTGGGCATGGGTCAAAA |
| mm4__chr14 102.46 Mbp_chr14_102461655 |
AGCAAGCCCCAGATATCAGCGACTTCACACAGTGAACCGTGGCCCCCGCTCCACGAAGCTGGCCCTGCATCTGTCCTCTT CTAGGGCAGTGGTCCTCCAGGTGGTGGCTTCCTGATCCTGCCTGCTTTG |
| mm4__chr9 133.25 Mbp_chr9_133252122 |
AGAGGGGCAAGGAAGCAAAAATGACTCAAGCCCACAGAGGCAGCAGCTCTCCCTGTGCTGGGCACATGCCCAAGGCTGGC TTTGGGGACAAAGAATAATCAAAGATGACACTTTGGGGCTGC |
| mm4__chr19 58.56 Mbp_chr19_58563366 |
GGGTCCTGTTCACTCCTCAGATCGCTGGGGAGCAGAAGCGCATGCGCAAAGCCACCTTCGCGGGAGGGTGATTGGATCTG GCCAGAAAGGCCAGCCATGCCGAGGTCCCAGGGGACGTGGAGGTGAGGGCTTACAC |
| mm3__chrX 46.29 Mbp_chrX_46289061 |
TGGAGGGGAAGACAACTGGGGGGCATCATCGCCCATGAAGGCAGCCTTGCACATGCCAGAGCCTTTGTTGAAGACGAGAG CAGTGATATCATCATTCATGGTGATCTGGCAGTGGGTGTGGACTGGTGATGGAG |
| mm2__chr1 223.86 Mbp_chr1_223864784 |
TGACCCATGCTCACCATCACGCCCTGGTGCCTGGGGCGCCCCACCATGGAAGGGAAGATGGCCCAGGGGTCATCATCCCC TGTGAAGCCAGCCTTGCACATGCCGGAGCTGTTGTCAATGACGAGCG |
| mm4__chr9 131.07 Mbp_chr9_131069729 |
CTCTAAACCCAGCACGCACTGCCCCTGGCCCCTCTAGCCTGCAGGTGCTCCCCACTGGGCGCTGCCTCGGCCCAGTGCCA CGAGAACGGCACATGCGTGTGCAGGCCTGGCTTCGAGGGCTACAAATGTGACCGCTGC |
| mm4__chr5 67.02 Mbp_chr5_67019260 |
TCCTGGGGCACATTGGAAAGGCGTCCAGAGGCCGCTGTGCTAATTAATGCGCAGCTGCACCCATTGTGGGCATGTCCACA GCCGGCTTCCTGGTATGCAGTGGAGATGGGCCCATTGACACT |
| mm4__chr2 97.10 Mbp_chr2_97095824 |
GGATGACAGGAGGTGGAAGTGAGAAGGAAGCAGGTACAAGGCCGGCTCCTGGGTCTCTAGCCCGGTAGGTGCAGGGCCGT TCATGGAGACAGGGCTT |
| mm4__chr9 86.68 Mbp_chr9_86680530 |
AGACCATGTGAATAGGAAGGCAAAATAACCTTCTATTAAACCTGGAAGCTGGCTTTGCTCATGTTTGTTTGCATTAGATA AGATATGACTGCTAATTGATTTGGAAATCAAAGTTGTTGCATTGATTTGCGTTTGGT |
| mm2__chr18 62.44 Mbp_chr18_62442052 |
CCTCGTCATCGACAATGGCTCTGGCATGTGCAGGGCTGGCTTCGCAGGCGACAGTGCTCTCCAGGCCGTCTTCCTCTCCA TCATGGGGCACCCCAGGCACCAGGGTGTGATGGTGGGCATGGGTCAGAAGGACTC |
| mm1__chr9 100.73 Mbp_chr9_100731283 |
GCCAGCTCACCATGGATGATGATATCGCCCCACTTGTCATTGACAACGGCTCAGGCATGTGCAAGGCCGGCTTTGCAGGT GATAATGTCCCCCTGGGCCATCTTCCCCCTCCATCGTGGAGCTCCCTAG |
| mm1__chr22 15.72 Mbp_chr22_15721530 |
GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC CATCGTGGGGTGCCCCAGGCACCAGAACATGAT |
| mm1__chr14 19.40 Mbp_chr14_19402841 |
ATCATGTTCTGGTGCCTGGGGCACCCCACGATGGAAGGGAAGACAGCCCGGGGGGCATCGTCACCTGCAAAGCCGGCCTT GCACATGCCAGAGCCGTTGTCAATGACGAGCAC |
| mm1__chr14 19.00 Mbp_chr14_18998911 |
GTGCTCGTCATTGACAACGGCTCTGGCATGTGCAAGGCCGGCTTTGCAGGTGACGATGCCCCCCGGGCTGTCTTCCCTTC CATCGTGGGGTGCCCCAGGCACCAGAACATGAT |
| mm4__chr11 133.55 Mbp_chr11_133554323 |
TAGGCTGTCAACGTCCAAGCCATAGCTTGAGTTTCCAAGTCAAACTTCCTTTTCCCCAGATAAAGGAAACACTGGGAAGG AAACAATGGTTAGATTAGCAAGGCCGGCCTCGAGGCTACCGGGAACGAATAGCGCTAG |
| mm4__chr7 2.56 Mbp_chr7_2559194 |
CGAGCCAGCAACCCTGAGGGGCGGCCGGGCAGCGCCGCCACCATGTTCCTGGGCACCGGGGAGCCGGCCTTGGACACGGT AAGAACGGGCGAGGGG |
| mm4__chr22 50.05 Mbp_chr22_50045601 |
CTCCCTGCAGTGTCTCTGTGACTGCGGCCGTCCAGCTGCACCCCTCGGCCTCCGACCACGGAGCCGGCCTTGCCCCAGCT GCTCGCCGCGCTGTCCACAAGCCTAGCACAGAACCACAC |
| mm4__chr11 64.62 Mbp_chr11_64617873 | CAGTGGTGCAAATGTGGACGTGTAAGCATGTGCGATGCAGGCTTAGGGGAGAGGCTGTGGCTTCTGGCAGTTTCCTCT |
| mm4__chr11 60.92 Mbp_chr11_60921930 |
CGTCTACTGGCTGCTGTCTCTGCTCCTCGGCTTGGTCTTGGCCTTGCTGGGGCGGATCCTGTGGGGCCTGAAGCTTGTCA TCTTCCTGGCCGGCTTCGTGGCCCTGATGAGGTCGGTGCCTG |
| mm2__chr9 6.83 Mbp_chr9_6834499 |
CCGCTACCAGCTCACCATAGATGATGATATTGCTACGCCCGTCGTCGACAACGGCTCCGGCATGTGGAAGGCTGGCTTCG CAGGTGACGATGACCCCCGGCCGTCTTCCCCTTCATCGTGT |
| mm4__chr9 137.16 Mbp_chr9_137160287 |
GATGAAGGGAGGCACGGGTGGGTGGCCCCACGGGTCCCAGGTCCTGTGCAGGTGCAGGGTCGGCTTTGTGGACATGCCCC TGTCCTCGTGGCACAGCAGGGTGGGGGTCAGCCTGCAGGCTGGGCTGTTTCTCA |
| mm4__chr14 100.82 Mbp_chr14_100824536 |
TTCTTGACGTCTGTGGCTGGTGCGGGCGTTTCTGCTGTGCAAGGCCTGCTTCCGGGGCTCTAGTCCCTGGGGCTCCTGGC AAGCTCCACAGGCTGTAAAGGGG |
| mm4__chr22 30.71 Mbp_chr22_30712840 |
ACAGGTTGTATCTTGGGGTCATTATGGACATAACCTCAGCTCTTTATTCTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCAGTAAAGGAAGGAGCCCTGGT |
| mm4__chr16 27.77 Mbp_chr16_27774151 |
GCCCACCACAGTTAGGGAAATACCAGGGCAAGTCGAACCCCAAAGCCTGCCTTGCATCTGTTTGGGGTCCCTGGCTTCCA GTTGGATCCTTTTCTGCCTTCCCCTGGTG |
| mm2__chr2 73.90 Mbp_chr2_73901358 |
GTGTGAAGAGGAGACCACCGCGCTCGTGTGTGACAATGGCTCTGGCCTGTGCAAGGCAGGCTTCGCAGGAGATGATGCCC CCCGGGCTGTCTTCCCCTCCATTGTGGG |
| mm3__chr10 110.51 Mbp_chr10_110508586 |
TTGCTTGCCCTGCTAATGGAAGGAGAGACTGGGTCTCCCCAGTGAGCCATGTGCTGAATATATGCTGACCCCTTCCAAGG GAAGAGGGCCATGTGGAAGGCAGGCTCCGAGGTGTCGAGTGGGCTCCAGAGGCAATAC |
| mm4__chr10 119.44 Mbp_chr10_119436742 |
CGAGACCAAGGATGCACTGTGCTTGGTCCTGACCATCATGAATGGGGGTGACCTGAAGTTCCACATCTACAACATGGGCA ACCCTGGCTTCGAGGAGGAGCGGGCCTTGTTTTATGC |
| mm4__chr5 131.66 Mbp_chr5_131660048 |
ATGCCCATCATCACACCCTGGTGCCTCGGGTGCCCCATTATGGAGGGGAAGACGGCCCGGGGGCATCGTTGCCCGTGAAG CAGACCTTCCACATGCCAGAGCCATTGTCGACGACA |
| mm4__chr19 18.79 Mbp_chr19_18788710 |
AGTCTGCATGCTCGTGGCACTCGCTGGGCGAGCCGTCGGGGCAGAAGCGCTGTGCGCGCCGCTGGCAGCCGGACGCCTGG TCGCCCACGAAGCCGGGCTGGCACGGGCCGCACTGGAAGGAGCCCTG |
| mm4__chr15 77.57 Mbp_chr15_77572058 |
ATCGTGGTGTCACTTGGCATAGCAAGAAGCATCCCCCAGGATCTCGACTGGCGTTGGAGTGGTGTGGGGCCCAGGTAGAG TTGGGCCAGCAGGTCCAAGTCCGGCTTTGGGGTCAAAGGGCAAGGAGAATGGCCAGAGA |
| mm4__chr7 44.31 Mbp_chr7_44308785 |
CATGAGGCACAGAGAAGGCACTTTGCCCAGCAGCAGGCCTTGCACATCAGGCGGTGTGCGAGCCCCTCGTCTCTCCTCAC AAGAGACCAGGGG |
| mm4__chr1 40.65 Mbp_chr1_40649154 |
GGCTCCTCTCCTAGTTGTGCAGACCTTTGTCTCCCCGAAGCCAGGCTGGCACGTGCATGNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNTGCAGGAAGAGACATGGGGCAC |
| mm4__chr2 120.87 Mbp_chr2_120874522 |
GCGTCAGCATTTGGGGAAAGACGGATGTGCCTGGAAGCGGGCCTTGCTCCTGTCTGTGTAGACAGGCCGGCGTTGAATGT CTACGGGTGGCATCTATCATCCG |
| mm4__chr2 102.48 Mbp_chr2_102479311 |
CGTGAACGAGCAGCTCTACATGATGATCTTTGGGGAGGCCCTGCTCAATGATGGCATTACTGTGGTGAGATGTCATGTGC CCGCCCGGCTTCCGGGGGAGATGAGGGTTCGGGGTG |
| mm4__chr2 91.59 Mbp_chr2_91588366 |
CAGACACAGTCTCCTTCCGGGAGCAGGCAGGTGCAAGGCAGGCACCGGGGCTTTGGAAGGGGCTGGTGGAGATGCTTGGG TGCCGGTAGCCCCAGGGCAGACCCTGGGGCCTCCCTCATTCCAGCTCCTCTGC |
| mm4__chr1_KI270712v1_random 131.35 Kbp_chr1_KI270712v1_random_131352 |
ACTTAATGGCACCCGAGCAGGGCGCCCTGGCAGACGCAGTCTCCTTCCGGGAGCAGGCAGGTGCAAGGCAGGCACCGGGG CTTTGGAAGGGGCTGGTGGAGATG |
| mm4__chr1 144.79 Mbp_chr1_144787685 |
CATCTCCACCAGCCCCTTCCAAAGCCCCGGTGCCTGCCTTGCACCTGCCTGCTCCCGGAAGGAGACTGCGTCTGCCAGGG CGCCCTGCTCGGGTGCCATTAAGT |
| mm4__chr1 144.18 Mbp_chr1_144178679 |
ACTTAATGGCACCCGAGCAGGGCGCCCTGGCAGACGCAGTCTCCTTCCGGGAGCAGGCAGGTGCAAGGCAGGCACCGGGG CTTTGGAAGGGGCTGGTGGAGATG |
| mm4__chr1 143.35 Mbp_chr1_143353094 |
ACTTAATGGCACCCGAGCAGGGCGCCCTGGCAGACGCAGTCTCCTTCCGGGAGCAGGCAGGTGCAAGGCAGGCACCGGGG CTTTGGAAGGGGCTGGTGGAGATG |
| mm4__chr15 89.22 Mbp_chr15_89221817 |
AAAGCCCTCCTTTGTGTGCTCCTGCTCTGGAGTTCCTGCTCGGCCATGTGGAAGCCCGGCTGTGGGGCTGGGATCTGGGC CAGTCCCATTCCCTCTTT |
| mm4__chr22 49.01 Mbp_chr22_49009568 |
AGTCTCCCCTGCTTCCTTCTCTGGAAATTGGCATGAACCGTGTGCCAGGCCGGCTTGTAGGCTTGCAGGGAGGGTGGGTG GATTCGCGCCTGGTTGCCAGCAGCCCCCGGCAAGGGACTGGTGAATT |
| mm4__chr9 127.22 Mbp_chr9_127219504 |
CAAGGGTCCCTCGCATTCTTTTACTGTAAACAAACAATGCCTTAAATTGTGTCTTGTTTTCTGTTCCTATGGGTGCTATT CATCTGGAAGGCCTGCTTCCAGGCCTCTTTGCTGTCAGCCCTTCTGAGACAGGAC |
| mm4__chr16 14.37 Mbp_chr16_14372616 |
CAGCAGCCCCACACAAAGGAAGTGCAAGGCCGCCTTGGAGGGCAACGGCTCCCCTGGGCCTCCCCTCCCCTGCCTGCAGG CAGCTCCACTT |
| mm4__chr4 8.77 Mbp_chr4_8773760 |
CGAGCCCTGCAGATGAAGGGGCGCAGCCAGCAGAGGGGGACACGGGAGGACAGCATGTGCAAGGCCGGAGCCCAGGGACA CGTGGCTGCGGTGGCATCCCGCCCGGCCCCCGTGCCTCTGGGTAACGTGTG |
| mm4__chr17 73.44 Mbp_chr17_73436200 |
GTCGAAGAGCACGTAGAGGGTCCAGTGTTCCCTGGGGGGCACTTGGAAGCCCCCCATACCGGCTTTGCACATGGAGGTGA AAGGAGGAGGCTCCTCTCCTCGCTCTCAGCCTGGCACATTGTT |
| mm4__chr10 133.48 Mbp_chr10_133475298 |
AGCCACACTCCTCACTCAGTGAGTGGCTGCAACCCTCCATGCCTGCCTTCCACATGGATGACCCACCTAATTTTCCTTTC AATGTTTTTGAAAAGTCAGATCTGATTCTACCCTGCCCTGACCT |
| mm4__chr12 122.97 Mbp_chr12_122974134 |
CAAACTGCTGCAAACGGAGAGGGGGTGAGGGTGGGGTGGGGTGCTGCATGTGCCAGGCCTGCCTGGTGGGTGGCTGGGGA GCCGACCTGCCCTGGGCCTCCTGGGGAAAAAGGGCAGTTAGGCGGGTAATGA |
| mm4__chr10 15.30 Mbp_chr10_15295741 |
GCATTTTATGGGCTGGCCACTGGGTCTGGGTAGAAGAAAGGCCCATTCCCACCAAGCAGGCCATGCAAATGCTCTCACCC CAAGTAGCCCAGCACTCCTG |