CRISPOR

Micro-homology scoring of potential deletions

Guide: GCCCCCGCCCTGGCCACTTC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	(Wild-type sequence)
364.2	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGC----------GCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`10 bp deleted → frameshift`
298.2	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCC--------------GAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`14 bp deleted → frameshift`
294.4	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCC--------------------GAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`20 bp deleted → frameshift`
244.2	`GCGCGCTGTGAGCCGAGGTCGCCCCCGC------------------GCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`18 bp deleted → no frameshift`
219.6	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGC------------GCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`12 bp deleted → no frameshift`
218.4	`GCGCGCTGTGAGCCGAGGTCGCC--------------------------GAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`26 bp deleted → frameshift`
207.2	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCC---------------------------GCCAGGGGGCGCCGGCGC`	`27 bp deleted → no frameshift`
191.4	`GCGCGCTGTGAGCCGAG-----------------------------------TCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`35 bp deleted → frameshift`
190.2	`GCGCGCTGTGAGCCGAGGTCGCCCCCG-----------------------AGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`23 bp deleted → frameshift`
188.8	`GCGCGCTGTGAGCCGAGGTCGCCCCCG---------------GCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`15 bp deleted → no frameshift`
180.6	`GCGCGCTGTGAGCCGAGGTCGC------------------------GCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`24 bp deleted → no frameshift`
179.6	`GCGCGCTGTGAGCCGAGGTCGCCCCCGC----------------GCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`16 bp deleted → frameshift`
165.0	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCC------------------------------------AGGGGGCGCCGGCGC`	`36 bp deleted → no frameshift`
162.8	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTT------------------AGGCCGCCAGGGGGCGCCGGCGC`	`18 bp deleted → no frameshift`
156.1	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCA------------------------------GGGGGCGCCGGCGC`	`30 bp deleted → no frameshift`
154.8	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCC-------------------GAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`19 bp deleted → frameshift`
149.1	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTC--------------CTTAGGCCGCCAGGGGGCGCCGGCGC`	`14 bp deleted → frameshift`
147.2	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCC--------------------TTAGGCCGCCAGGGGGCGCCGGCGC`	`20 bp deleted → frameshift`
146.4	`GCGCGC------------------------------------------CGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`42 bp deleted → no frameshift`
143.5	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCT-------------------------TAGGCCGCCAGGGGGCGCCGGCGC`	`25 bp deleted → frameshift`
140.0	`GCGCGCTGTGAGCCGAGGTCG---------------------GCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`21 bp deleted → no frameshift`
133.2	`GCGCGCTGTGAGCCGAGGTCGC----------------------GCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`22 bp deleted → frameshift`
120.4	`GCGCGCTGTGAGCCGAGGTCGCCCC------------------------GAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`24 bp deleted → no frameshift`
115.2	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCC---------------------------------GCCAGGGGGCGCCGGCGC`	`33 bp deleted → no frameshift`
114.8	`GCGCGCTGTGAGCCGAGGTCGCCC-------------------------GAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`25 bp deleted → frameshift`
114.8	`GCGCGCTGTGAGCCGAGG-------------------------CGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`25 bp deleted → frameshift`
109.2	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCC--------------------------TTAGGCCGCCAGGGGGCGCCGGCGC`	`26 bp deleted → frameshift`
108.0	`GCGCGC----------------------------------------GCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`40 bp deleted → frameshift`
103.6	`GCGCGCTGTGAGCCG---------------------------GCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`27 bp deleted → no frameshift`
97.6	`GCGCGCTGTGAGCCGAGGTCGCC------------------------------------------AGGGGGCGCCGGCGC`	`42 bp deleted → no frameshift`
94.0	`GCGCGCTGTGAGCCGAGGTCGCCCCC-----------------------------TTAGGCCGCCAGGGGGCGCCGGCGC`	`29 bp deleted → frameshift`
94.0	`GCGCGCTGTGAGCCGAGGTCG-----------------------------AGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`29 bp deleted → frameshift`
91.5	`GCGCGCTGTGAGCCGAGGTC----------------------------------CTTAGGCCGCCAGGGGGCGCCGGCGC`	`34 bp deleted → frameshift`
90.0	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGC--------------------------------------GCCGGCGC`	`38 bp deleted → frameshift`
89.2	`GCGCGCTGTGAGCCGAGGTCGCCCC------------------------------TTAGGCCGCCAGGGGGCGCCGGCGC`	`30 bp deleted → no frameshift`
89.2	`GCGCGCTGTGAGCCG------------------------------CGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`30 bp deleted → no frameshift`
88.8	`GCGC--------------------------------------------CGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`44 bp deleted → frameshift`
85.2	`GCGCGCTGTGAGCCGAGGTCGCC---------------------------------------GCCAGGGGGCGCCGGCGC`	`39 bp deleted → no frameshift`
84.8	`GCGCGCTGTGAGCCGAGGTCGCCC-------------------------------TTAGGCCGCCAGGGGGCGCCGGCGC`	`31 bp deleted → frameshift`
84.8	`GCGCGCTGTGAGC-------------------------------GCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`31 bp deleted → frameshift`
81.0	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCC----------------------------------------GGCGC`	`40 bp deleted → frameshift`
80.8	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCC--------------------------------GCCAGGGGGCGCCGGCGC`	`32 bp deleted → frameshift`
80.8	`GCGCGCTGTGAGCCGAGGTCGCC--------------------------------TTAGGCCGCCAGGGGGCGCCGGCGC`	`32 bp deleted → frameshift`
80.8	`GCGCGCTGTGAGCCG--------------------------------CCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`32 bp deleted → frameshift`
80.0	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCC----------------------------------------------GGCGC`	`46 bp deleted → frameshift`
76.8	`GCGCGCTGTGAGC---------------------------------GCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`33 bp deleted → no frameshift`
72.8	`GCGCGCTGTGAGCCG------------------------------------------------CCAGGGGGCGCCGGCGC`	`48 bp deleted → no frameshift`
69.6	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGG-----------------------------------GGGCGCCGGCGC`	`35 bp deleted → frameshift`
69.6	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCC-----------------------------------AGGGGGCGCCGGCGC`	`35 bp deleted → frameshift`
67.5	`GCGCGCTGTGAG----------------------------------------TCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`40 bp deleted → frameshift`
66.6	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGC--------------------------------------------GC`	`44 bp deleted → frameshift`
66.0	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGG------------------------------------GGCGCCGGCGC`	`36 bp deleted → no frameshift`
62.8	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGG-------------------------------------GCGCCGGCGC`	`37 bp deleted → frameshift`
62.8	`GCGCGCTGTGAGCCGAGGTCGCCCC-------------------------------------GCCAGGGGGCGCCGGCGC`	`37 bp deleted → frameshift`
62.8	`GCGCG-------------------------------------GCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`37 bp deleted → frameshift`
61.0	`GCGCGCTGTGAGCCGAGG------------------------------------------CCGCCAGGGGGCGCCGGCGC`	`42 bp deleted → no frameshift`
60.0	`GCGCGCTGTGAGCCGAGGTCGCCC--------------------------------------GCCAGGGGGCGCCGGCGC`	`38 bp deleted → frameshift`
60.0	`GCGCGC--------------------------------------GCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`38 bp deleted → frameshift`
59.2	`GCGCGCTGTGAGCCGAGGTCGCC----------------------------------------------------GGCGC`	`52 bp deleted → frameshift`
56.8	`GCGCGCTGTGAGCCGAGGTCGCCCCC---------------------------------------AGGGGGCGCCGGCGC`	`39 bp deleted → no frameshift`
56.8	`GCG---------------------------------------GCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`39 bp deleted → no frameshift`
54.0	`GCGCGCTGTGAGCCGAGGTCGCCCC----------------------------------------AGGGGGCGCCGGCGC`	`40 bp deleted → frameshift`
51.6	`GCGCGCTGTGAGCCGAGGTCGCCC-----------------------------------------AGGGGGCGCCGGCGC`	`41 bp deleted → frameshift`
51.6	`GCGCGCTGTGAGCC-----------------------------------------TTAGGCCGCCAGGGGGCGCCGGCGC`	`41 bp deleted → frameshift`
51.6	`GCGCGCTGTGAGCCGAGGTCGCCCCCG-------------------------------------------------GCGC`	`49 bp deleted → frameshift`
46.8	`GCGCGCTGTGAGCC---------------------------------------------------AGGGGGCGCCGGCGC`	`51 bp deleted → no frameshift`
44.4	`GCGCGCTGTGAGCCGAGGTCGCCCCCGC--------------------------------------------GCCGGCGC`	`44 bp deleted → frameshift`
44.4	`GCGCGCTGTGAGCCGAGGTCGCCCCCGC----------------------------------------------------`	`52 bp deleted → frameshift`
42.0	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCC---------------------------------------------GGCGC`	`45 bp deleted → no frameshift`
42.0	`GCGCG---------------------------------------------AGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`45 bp deleted → no frameshift`
41.0	`GCGCGCTGTGAGCCGAGG--------------------------------------------------GGGCGCCGGCGC`	`50 bp deleted → frameshift`
40.0	`GCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGC----------------------------------------------`	`46 bp deleted → frameshift`
40.0	`GC----------------------------------------------CGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`46 bp deleted → frameshift`
38.0	`GCG-----------------------------------------------AGTCCTTAGGCCGCCAGGGGGCGCCGGCGC`	`47 bp deleted → frameshift`
37.6	`GCGCGCTGTGAGCCG-------------------------------------------------------------GCGC`	`61 bp deleted → frameshift`
33.3	`GCGCGCTGT--------------------------------------------CCTTAGGCCGCCAGGGGGCGCCGGCGC`	`44 bp deleted → frameshift`
33.0	`GCGCGCTGTGAGCCGAGGTCGC----------------------------------------------------------`	`58 bp deleted → frameshift`
33.0	`GCGCGC----------------------------------------------------------CAGGGGGCGCCGGCGC`	`58 bp deleted → frameshift`
32.8	`GCGCGCTGTGAGCCGAGGTCGCCCCCGC--------------------------------------------------GC`	`50 bp deleted → frameshift`
32.8	`GCGCGCTGTGAGCCGAGGTCGCCCC--------------------------------------------------GGCGC`	`50 bp deleted → frameshift`
32.8	`GCGCGCTGTGAGCCGAGGTCGC--------------------------------------------------GCCGGCGC`	`50 bp deleted → frameshift`
31.2	`GCGCGCTGTGAGCCGAGGTCGCCC---------------------------------------------------GGCGC`	`51 bp deleted → no frameshift`
31.2	`GCGCGCTGTGAGCCGAGG---------------------------------------------------GGCGCCGGCGC`	`51 bp deleted → no frameshift`
30.0	`GCGC------------------------------------------------------------CAGGGGGCGCCGGCGC`	`60 bp deleted → no frameshift`
29.6	`GCGCGCTGTGAGCCGAGG----------------------------------------------------GCGCCGGCGC`	`52 bp deleted → frameshift`
28.5	`GCGCGCTGTGAG-----------------------------------------------GCCGCCAGGGGGCGCCGGCGC`	`47 bp deleted → frameshift`
28.4	`GCGCGCTGTGAGCCGAGG-----------------------------------------------------CGCCGGCGC`	`53 bp deleted → frameshift`
26.4	`GCGCGC--------------------------------------------------------------------CGGCGC`	`68 bp deleted → frameshift`
25.8	`GCGCGCT-------------------------------------------------TAGGCCGCCAGGGGGCGCCGGCGC`	`49 bp deleted → frameshift`
25.6	`GCGCGCTGTGAGCCGAGGTCG-------------------------------------------------------GCGC`	`55 bp deleted → frameshift`
25.6	`GCGCGC-------------------------------------------------------CGCCAGGGGGCGCCGGCGC`	`55 bp deleted → frameshift`
24.4	`GCGCGCTGTGAGCCGAGGTCGC--------------------------------------------------------GC`	`56 bp deleted → frameshift`
24.0	`GCGC----------------------------------------------------------------------CGGCGC`	`70 bp deleted → frameshift`
23.2	`GCGC---------------------------------------------------------CGCCAGGGGGCGCCGGCGC`	`57 bp deleted → no frameshift`
22.0	`GCGCGCTGTGAGCCG----------------------------------------------------------CCGGCGC`	`58 bp deleted → frameshift`
20.8	`GCGCGCTGTGAGCCGAGG-----------------------------------------------------------CGC`	`59 bp deleted → frameshift`
20.8	`GCGCGCTGTGAGC-----------------------------------------------------------GCCGGCGC`	`59 bp deleted → frameshift`
20.8	`GC-----------------------------------------------------------CGCCAGGGGGCGCCGGCGC`	`59 bp deleted → frameshift`
20.0	`GCGCGC--------------------------------------------------------------------------`	`74 bp deleted → frameshift`
19.2	`GCGCGCTGTGAG-------------------------------------------------------GGGGCGCCGGCGC`	`55 bp deleted → frameshift`
18.0	`GC--------------------------------------------------------------CAGGGGGCGCCGGCGC`	`62 bp deleted → frameshift`
17.6	`GCGC----------------------------------------------------------------------------`	`76 bp deleted → frameshift`
16.4	`GCGCGCTGTGAGCCG----------------------------------------------------------------C`	`64 bp deleted → frameshift`
15.6	`GCGCGCTGTGAGC-----------------------------------------------------------------GC`	`65 bp deleted → frameshift`
14.8	`GCGCGC------------------------------------------------------------------GCCGGCGC`	`66 bp deleted → no frameshift`
14.0	`GCGCGCTGTGAGC-------------------------------------------------------------------`	`67 bp deleted → frameshift`
11.6	`GCGCG-----------------------------------------------------------------------GCGC`	`71 bp deleted → frameshift`
10.8	`GCGCGC------------------------------------------------------------------------GC`	`72 bp deleted → no frameshift`
10.8	`GC------------------------------------------------------------------------CGGCGC`	`72 bp deleted → no frameshift`
10.4	`GCG-------------------------------------------------------------------------GCGC`	`73 bp deleted → frameshift`
8.0	`GC------------------------------------------------------------------------------`	`78 bp deleted → no frameshift`

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