CRISPOR

Micro-homology scoring of potential deletions

Guide: CAACGGCTCCGGCATGTGCA AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	(Wild-type sequence)
472.0	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGC---------------TTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`15 bp deleted → no frameshift`
346.2	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGC-----------CGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`11 bp deleted → frameshift`
315.0	`ATCGCCGCGCTCGTCGTCGACAACGGCT---------------------TCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`21 bp deleted → no frameshift`
311.6	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGC-----CGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`5 bp deleted → frameshift`
256.2	`ATCGCCGCGCTCGTCGTCGACAACGGC-----------------CGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`17 bp deleted → frameshift`
255.2	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGC---------TTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`9 bp deleted → no frameshift`
198.8	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGC--------------GGGCGACGATGCCCCCCGGGCCGTCTT`	`14 bp deleted → frameshift`
190.2	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGG-----------------------GCGACGATGCCCCCCGGGCCGTCTT`	`23 bp deleted → frameshift`
180.6	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGC------------------------GACGATGCCCCCCGGGCCGTCTT`	`24 bp deleted → no frameshift`
162.8	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGC------------------GACGATGCCCCCCGGGCCGTCTT`	`18 bp deleted → no frameshift`
147.2	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGC--------------------GGGCGACGATGCCCCCCGGGCCGTCTT`	`20 bp deleted → frameshift`
141.0	`ATCGCCGCGCTCGTCGTCGACAACGG-----------------------------GCGACGATGCCCCCCGGGCCGTCTT`	`29 bp deleted → frameshift`
140.0	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCG---------------------CGGGCGACGATGCCCCCCGGGCCGTCTT`	`21 bp deleted → no frameshift`
136.5	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGC--------------------------CCCCCGGGCCGTCTT`	`26 bp deleted → frameshift`
133.8	`ATCGCCGCGCTCGTCGTCGACAACGGC------------------------------GACGATGCCCCCCGGGCCGTCTT`	`30 bp deleted → no frameshift`
113.6	`ATCGCCG---------------------------------------GCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`39 bp deleted → no frameshift`
109.2	`ATCGCCGCGCTCGTCGTCGACAACGGC--------------------------GGGCGACGATGCCCCCCGGGCCGTCTT`	`26 bp deleted → frameshift`
108.0	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGG----------------------------------------GCCGTCTT`	`40 bp deleted → frameshift`
103.6	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCG---------------------------ACGATGCCCCCCGGGCCGTCTT`	`27 bp deleted → no frameshift`
103.6	`ATCGCCGCGCTCGTCGTCGACAACG---------------------------CGGGCGACGATGCCCCCCGGGCCGTCTT`	`27 bp deleted → no frameshift`
103.6	`ATCGCCGCGCTCGTCGTCG---------------------------GCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`27 bp deleted → no frameshift`
99.9	`ATCGCCGCGCTCGTCGTCGACAACGGCTC----------------------GCGGGCGACGATGCCCCCCGGGCCGTCTT`	`22 bp deleted → frameshift`
99.4	`ATCGCCGCGCTCGTCGTCGAC---------------------------------------GATGCCCCCCGGGCCGTCTT`	`39 bp deleted → no frameshift`
98.8	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATG----------------------------CCCCCCGGGCCGTCTT`	`28 bp deleted → frameshift`
96.0	`ATCGCCGCGCTCGTCGTCG---------------------------------CGGGCGACGATGCCCCCCGGGCCGTCTT`	`33 bp deleted → no frameshift`
89.2	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCG------------------------------ATGCCCCCCGGGCCGTCTT`	`30 bp deleted → no frameshift`
89.2	`ATCGCCGCGCTCGTCG------------------------------GCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`30 bp deleted → no frameshift`
84.0	`ATCGCCGCG---------------------------------------------GGCGACGATGCCCCCCGGGCCGTCTT`	`45 bp deleted → no frameshift`
82.5	`ATCGCCGCGCTCGTCGTCGACAACG------------------------------------ATGCCCCCCGGGCCGTCTT`	`36 bp deleted → no frameshift`
82.5	`ATCGCCGCGCTCGTCG------------------------------------CGGGCGACGATGCCCCCCGGGCCGTCTT`	`36 bp deleted → no frameshift`
80.8	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGC--------------------------------CCCCCGGGCCGTCTT`	`32 bp deleted → frameshift`
77.4	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGC-----------------------------------------CGTCTT`	`41 bp deleted → frameshift`
76.8	`ATCGCCGCGCTCGTCGTCGACAACG---------------------------------ACGATGCCCCCCGGGCCGTCTT`	`33 bp deleted → no frameshift`
76.8	`ATCGCCGCGCTCG---------------------------------GCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`33 bp deleted → no frameshift`
75.0	`ATCGCCGCGCT--------------------------------------TCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`38 bp deleted → frameshift`
73.2	`ATCGCCGCGC----------------------------------CGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`34 bp deleted → frameshift`
71.0	`ATCGCCGCGCTCG---------------------------------------CGGGCGACGATGCCCCCCGGGCCGTCTT`	`39 bp deleted → no frameshift`
69.6	`ATCGCCGCGC-------------------------------------------GGGCGACGATGCCCCCCGGGCCGTCTT`	`43 bp deleted → frameshift`
69.6	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGC-----------------------------------CGTCTT`	`35 bp deleted → frameshift`
69.6	`ATCGCCGCGCTCGTCGTCG-----------------------------------GGCGACGATGCCCCCCGGGCCGTCTT`	`35 bp deleted → frameshift`
66.0	`ATCGCCGCGCTCGTCGTCGACAACGGCTCC------------------------------------CCCCGGGCCGTCTT`	`36 bp deleted → no frameshift`
66.0	`ATCGCCGC------------------------------------CGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`36 bp deleted → no frameshift`
63.7	`ATCGC------------------------------------------------GGGCGACGATGCCCCCCGGGCCGTCTT`	`48 bp deleted → no frameshift`
63.0	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCG---------------------------------------------TCTT`	`45 bp deleted → no frameshift`
62.8	`ATCGCCGCGCTCGTCGTCGACAACGGCTCC-------------------------------------CCCGGGCCGTCTT`	`37 bp deleted → frameshift`
62.8	`ATCGCCGCG-------------------------------------GCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`37 bp deleted → frameshift`
61.0	`ATCGCCGCGCTCGTCGTCGA------------------------------------------TGCCCCCCGGGCCGTCTT`	`42 bp deleted → no frameshift`
60.0	`ATCGCCGCGCTCGTCGTCGACAACGGCTCC--------------------------------------CCGGGCCGTCTT`	`38 bp deleted → frameshift`
60.0	`ATCGCCGCGCTCGTCGTCGACAACGGC--------------------------------------CCCCCGGGCCGTCTT`	`38 bp deleted → frameshift`
60.0	`ATCGCCGCGCTCGTCGTCGACAACGG----------------------------------------------GCCGTCTT`	`46 bp deleted → frameshift`
60.0	`ATCGCCGCGCTCGTCG--------------------------------------GGCGACGATGCCCCCCGGGCCGTCTT`	`38 bp deleted → frameshift`
57.0	`ATCGCCGCGCTCGTCGTCGACAACGGC-----------------------------------------------CGTCTT`	`47 bp deleted → frameshift`
56.8	`ATCGCCGCGCTCGTCGTCGACAACGGCTCC---------------------------------------CGGGCCGTCTT`	`39 bp deleted → no frameshift`
54.0	`ATCGCCGC----------------------------------------TTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`40 bp deleted → frameshift`
51.6	`ATCGCCGCGCTCG-----------------------------------------GGCGACGATGCCCCCCGGGCCGTCTT`	`41 bp deleted → frameshift`
51.6	`ATCGCCGCG-------------------------------------------------ACGATGCCCCCCGGGCCGTCTT`	`49 bp deleted → frameshift`
48.8	`ATCGCCGCGCTCGTCG------------------------------------------ACGATGCCCCCCGGGCCGTCTT`	`42 bp deleted → no frameshift`
48.8	`ATCG------------------------------------------GCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`42 bp deleted → no frameshift`
46.4	`ATCGC-------------------------------------------TTCGCGGGCGACGATGCCCCCCGGGCCGTCTT`	`43 bp deleted → frameshift`
42.0	`ATCGCCGCGCTCGTCG---------------------------------------------ATGCCCCCCGGGCCGTCTT`	`45 bp deleted → no frameshift`
42.0	`ATCGCCGCGCTCG---------------------------------------------ACGATGCCCCCCGGGCCGTCTT`	`45 bp deleted → no frameshift`
40.5	`ATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGT----------------------------------------CTT`	`40 bp deleted → frameshift`
38.0	`ATCGCCGCGC-----------------------------------------------GACGATGCCCCCCGGGCCGTCTT`	`47 bp deleted → frameshift`
38.0	`ATCGCCG-----------------------------------------------GGCGACGATGCCCCCCGGGCCGTCTT`	`47 bp deleted → frameshift`
36.4	`ATCGCCGCGCTCG------------------------------------------------ATGCCCCCCGGGCCGTCTT`	`48 bp deleted → no frameshift`
35.0	`ATCGCCGCGCTCGTCGTC------------------------------------------------------------TT`	`60 bp deleted → no frameshift`
32.8	`ATCG--------------------------------------------------GGCGACGATGCCCCCCGGGCCGTCTT`	`50 bp deleted → frameshift`
31.2	`ATCGCCGCGCTCGTCGTCGACAACG---------------------------------------------------TCTT`	`51 bp deleted → no frameshift`
31.2	`ATCGCCG---------------------------------------------------ACGATGCCCCCCGGGCCGTCTT`	`51 bp deleted → no frameshift`
30.1	`ATCGCCGCGCTCGTC---------------------------------------------------------------TT`	`63 bp deleted → no frameshift`
30.0	`ATCGCC------------------------------------------------------------CCCCGGGCCGTCTT`	`60 bp deleted → no frameshift`
29.6	`ATCGCCGCGCTCGTCGTCG----------------------------------------------------GGCCGTCTT`	`52 bp deleted → frameshift`
29.6	`ATCGCCGCG----------------------------------------------------ATGCCCCCCGGGCCGTCTT`	`52 bp deleted → frameshift`
29.6	`ATCGC----------------------------------------------------GACGATGCCCCCCGGGCCGTCTT`	`52 bp deleted → frameshift`
26.8	`ATCGCCG------------------------------------------------------ATGCCCCCCGGGCCGTCTT`	`54 bp deleted → no frameshift`
26.8	`ATCG------------------------------------------------------ACGATGCCCCCCGGGCCGTCTT`	`54 bp deleted → no frameshift`
25.8	`ATCGCCGCGCTCGTCGTCGACAACGGCTC-------------------------------------------------TT`	`49 bp deleted → frameshift`
25.6	`ATCGCCGCGCTCGTCG-------------------------------------------------------GGCCGTCTT`	`55 bp deleted → frameshift`
25.6	`ATCGCCGCGC-------------------------------------------------------CCCCCGGGCCGTCTT`	`55 bp deleted → frameshift`
25.6	`ATCGCCG---------------------------------------------------------------------TCTT`	`69 bp deleted → no frameshift`
24.6	`ATCGCCG----------------------------------------------------------------GGCCGTCTT`	`64 bp deleted → frameshift`
23.4	`ATCGCCGCGCTCGTCGTCGACAACGGCT---------------------------------------------------T`	`51 bp deleted → no frameshift`
23.2	`ATCGCCGCGCTCGTCGTCG---------------------------------------------------------TCTT`	`57 bp deleted → no frameshift`
23.2	`ATCGCCGC---------------------------------------------------------CCCCCGGGCCGTCTT`	`57 bp deleted → no frameshift`
23.2	`ATCG---------------------------------------------------------ATGCCCCCCGGGCCGTCTT`	`57 bp deleted → no frameshift`
22.0	`ATCGCCGCGCTCG----------------------------------------------------------GGCCGTCTT`	`58 bp deleted → frameshift`
18.8	`ATCGCC-------------------------------------------------------------CCCGGGCCGTCTT`	`61 bp deleted → frameshift`
18.0	`ATCGCCGCG--------------------------------------------------------------GGCCGTCTT`	`62 bp deleted → frameshift`
18.0	`ATCGCC--------------------------------------------------------------CCGGGCCGTCTT`	`62 bp deleted → frameshift`
17.2	`ATCGCC---------------------------------------------------------------CGGGCCGTCTT`	`63 bp deleted → no frameshift`
16.4	`ATCGCCGCGC----------------------------------------------------------------CGTCTT`	`64 bp deleted → frameshift`
14.8	`ATCGCCGC------------------------------------------------------------------CGTCTT`	`66 bp deleted → no frameshift`
14.0	`ATCGCCGCG-------------------------------------------------------------------TCTT`	`67 bp deleted → frameshift`
14.0	`ATCG-------------------------------------------------------------------GGCCGTCTT`	`67 bp deleted → frameshift`
11.1	`ATCGCCGCGCTC------------------------------------------------------------------TT`	`66 bp deleted → no frameshift`
10.8	`ATCG------------------------------------------------------------------------TCTT`	`72 bp deleted → no frameshift`
9.9	`ATCGCCGCGCT--------------------------------------------------------------------T`	`68 bp deleted → frameshift`
9.4	`AT-------------------------------------------------------------GCCCCCCGGGCCGTCTT`	`61 bp deleted → frameshift`
7.2	`ATC---------------------------------------------------------------------------TT`	`75 bp deleted → no frameshift`

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