CRISPOR

Micro-homology scoring of potential deletions

Guide: GGCCAGGGCGGGGGCGACCT CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	(Wild-type sequence)
382.8	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGC---------TCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`9 bp deleted → no frameshift`
329.4	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGG------------CTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`12 bp deleted → no frameshift`
296.4	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCG------GCTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`6 bp deleted → no frameshift`
283.2	`GGACTCGGCGCGCCGGAAGTGGCCAGGGC---------------TCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`15 bp deleted → no frameshift`
269.4	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCG----------------CGCCCGGCTATTCTCGCAGCTCACCATG`	`16 bp deleted → frameshift`
244.2	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCG------------------CCCGGCTATTCTCGCAGCTCACCATG`	`18 bp deleted → no frameshift`
242.8	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGG----------CTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`10 bp deleted → frameshift`
233.7	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCT-----CACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`5 bp deleted → frameshift`
230.8	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG-----------CTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`11 bp deleted → frameshift`
210.0	`GGACTCGGCGCGCCGGAAGTGGC---------------------TCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`21 bp deleted → no frameshift`
199.8	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCG----------------------CGCCCGGCTATTCTCGCAGCTCACCATG`	`22 bp deleted → frameshift`
182.1	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGAC----------AGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`10 bp deleted → frameshift`
180.6	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCG------------------------CCCGGCTATTCTCGCAGCTCACCATG`	`24 bp deleted → no frameshift`
179.6	`GGACTCGGCGCGCCGGAAGTGGCCAGG----------------CTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`16 bp deleted → frameshift`
172.2	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGC-------------------------TATTCTCGCAGCTCACCATG`	`25 bp deleted → frameshift`
170.8	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC-----------------CGGCTATTCTCGCAGCTCACCATG`	`17 bp deleted → frameshift`
170.8	`GGACTCGGCGCGCC------------------------------------------CGGCTATTCTCGCAGCTCACCATG`	`42 bp deleted → no frameshift`
162.8	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC------------------GGCTATTCTCGCAGCTCACCATG`	`18 bp deleted → no frameshift`
155.4	`GGACTCGGCGCGCCGG---------------------------CTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`27 bp deleted → no frameshift`
150.5	`GGACTCGGCGCGCCGGAAGTGGCCAG------------------------CGCGCCCGGCTATTCTCGCAGCTCACCATG`	`24 bp deleted → no frameshift`
148.2	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGG----------------------------CTATTCTCGCAGCTCACCATG`	`28 bp deleted → frameshift`
147.2	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGC--------------------CCGGCTATTCTCGCAGCTCACCATG`	`20 bp deleted → frameshift`
139.2	`GGACTCGGC-----------------------------------TCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`35 bp deleted → frameshift`
133.2	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCG----------------------GCTATTCTCGCAGCTCACCATG`	`22 bp deleted → frameshift`
127.2	`GGACTCGGCGCGCCGGAAGTGGCCAGGGC-------------------------------TATTCTCGCAGCTCACCATG`	`31 bp deleted → frameshift`
121.2	`GGACTCGGCGCGCCGGAAGTGGCC--------------------------------CGGCTATTCTCGCAGCTCACCATG`	`32 bp deleted → frameshift`
109.2	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGG--------------------------CTATTCTCGCAGCTCACCATG`	`26 bp deleted → frameshift`
109.2	`GGACTCGGCGCGCCGGAAGTGGCCAGGGC--------------------------CCGGCTATTCTCGCAGCTCACCATG`	`26 bp deleted → frameshift`
108.0	`GGACTCGGCGCGC----------------------------------------GCCCGGCTATTCTCGCAGCTCACCATG`	`40 bp deleted → frameshift`
105.0	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCT---------------------ATTCTCGCAGCTCACCATG`	`21 bp deleted → no frameshift`
103.6	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGG---------------------------CTATTCTCGCAGCTCACCATG`	`27 bp deleted → no frameshift`
99.9	`GGACTCGGCGCGCCGGAAGTGGCCA----------------------CAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`22 bp deleted → frameshift`
98.8	`GGACTCGGCGCGCCGGAAGTGGC----------------------------GCGCCCGGCTATTCTCGCAGCTCACCATG`	`28 bp deleted → frameshift`
94.2	`GGACTCGGCGCGCCGGAAGTGGC-------------------------------------TATTCTCGCAGCTCACCATG`	`37 bp deleted → frameshift`
92.8	`GGACTCGGCGCGCCGG-------------------------------------------CTATTCTCGCAGCTCACCATG`	`43 bp deleted → frameshift`
89.2	`GGACTCGGCGCGCCGGAAGTGGC------------------------------GCCCGGCTATTCTCGCAGCTCACCATG`	`30 bp deleted → no frameshift`
89.2	`GGACTCGGCGCG------------------------------GCTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`30 bp deleted → no frameshift`
88.8	`GGACTCGGCGC--------------------------------------------CCGGCTATTCTCGCAGCTCACCATG`	`44 bp deleted → frameshift`
84.8	`GGACTCGGCGCGC-------------------------------TCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`31 bp deleted → frameshift`
81.9	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCT--------------------------CGCAGCTCACCATG`	`26 bp deleted → frameshift`
80.8	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCG--------------------------------CAGCTCACCATG`	`32 bp deleted → frameshift`
80.8	`GGACTCGGCGCGCCGGAAGTGGCCAGG--------------------------------CTATTCTCGCAGCTCACCATG`	`32 bp deleted → frameshift`
80.8	`GGACTCGGCG--------------------------------GCTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`32 bp deleted → frameshift`
76.8	`GGACTCGGCGCGCCGGAAGTGGCC---------------------------------GGCTATTCTCGCAGCTCACCATG`	`33 bp deleted → no frameshift`
76.8	`GGACTCGGCGC---------------------------------TCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`33 bp deleted → no frameshift`
75.0	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACC--------------------------------------ATG`	`38 bp deleted → frameshift`
73.2	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGC----------------------------------AGCTCACCATG`	`34 bp deleted → frameshift`
67.5	`GGACTC----------------------------------------ACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`40 bp deleted → frameshift`
63.6	`GGACTCGGCGCGCCGGAAG-------------------------------CGCGCCCGGCTATTCTCGCAGCTCACCATG`	`31 bp deleted → frameshift`
62.8	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGC-------------------------------------TCACCATG`	`37 bp deleted → frameshift`
62.8	`GGACTCGGCGCGCCG-------------------------------------CGCCCGGCTATTCTCGCAGCTCACCATG`	`37 bp deleted → frameshift`
62.4	`GGACTCGGC---------------------------------------------------TATTCTCGCAGCTCACCATG`	`51 bp deleted → no frameshift`
60.0	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCG--------------------------------------CAGCTCACCATG`	`38 bp deleted → frameshift`
60.0	`GGACTCGGCGCGC--------------------------------------GCGCCCGGCTATTCTCGCAGCTCACCATG`	`38 bp deleted → frameshift`
57.6	`GGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCT---------------------------------CACCATG`	`33 bp deleted → no frameshift`
56.8	`GGACTCGGCGCGCCG---------------------------------------CCCGGCTATTCTCGCAGCTCACCATG`	`39 bp deleted → no frameshift`
54.0	`GGACTCGGCGCGCCGGAAGTGGCCAGGGC----------------------------------------AGCTCACCATG`	`40 bp deleted → frameshift`
52.5	`GGACTCGGCGCGCCGGAAGTGGCCAG---------------------------------------------CTCACCATG`	`45 bp deleted → no frameshift`
51.6	`GG-----------------------------------------CTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`41 bp deleted → frameshift`
46.4	`GGACTCGGCGCGCCGGAAGTGGCCAGGGC-------------------------------------------TCACCATG`	`43 bp deleted → frameshift`
42.0	`GGACTCG---------------------------------------------CGCCCGGCTATTCTCGCAGCTCACCATG`	`45 bp deleted → no frameshift`
40.0	`GGACTCGGCGCGCCGGAAGTGGC----------------------------------------------AGCTCACCATG`	`46 bp deleted → frameshift`
40.0	`GGACTCGGCGCG----------------------------------------------GCTATTCTCGCAGCTCACCATG`	`46 bp deleted → frameshift`
40.0	`GGACTCGGC----------------------------------------------CCGGCTATTCTCGCAGCTCACCATG`	`46 bp deleted → frameshift`
38.0	`GGACTCGGCGCGC-----------------------------------------------TATTCTCGCAGCTCACCATG`	`47 bp deleted → frameshift`
38.0	`GGACTCG-----------------------------------------------CCCGGCTATTCTCGCAGCTCACCATG`	`47 bp deleted → frameshift`
36.6	`GGACTCGGCGCGC--------------------------------------------------------AGCTCACCATG`	`56 bp deleted → frameshift`
36.4	`GGACTCGGCG------------------------------------------------GCTATTCTCGCAGCTCACCATG`	`48 bp deleted → no frameshift`
35.5	`GGACTCGGCGCGCCGGAAGTGGCCA-----------------------------------------------------TG`	`53 bp deleted → frameshift`
34.4	`GGACTCGGCGCGCCGGAAGTGGC-------------------------------------------------TCACCATG`	`49 bp deleted → frameshift`
34.4	`GGACTCGGCGC-------------------------------------------------TATTCTCGCAGCTCACCATG`	`49 bp deleted → frameshift`
33.3	`GGAC--------------------------------------------AGCGCGCCCGGCTATTCTCGCAGCTCACCATG`	`44 bp deleted → frameshift`
33.0	`GGACTCGGCGC----------------------------------------------------------AGCTCACCATG`	`58 bp deleted → frameshift`
32.9	`GGACTCG-------------------------------------------------------------CAGCTCACCATG`	`61 bp deleted → frameshift`
28.4	`GGACTCGGCGCGCCG-----------------------------------------------------CAGCTCACCATG`	`53 bp deleted → frameshift`
24.6	`GGACTCGGCGCGCCGGAAGTGGCCA--------------------------------------------------CCATG`	`50 bp deleted → frameshift`
23.2	`GG---------------------------------------------------------CTATTCTCGCAGCTCACCATG`	`57 bp deleted → no frameshift`
22.2	`GGACTCGGCGCGCCGGAAG----------------------------------------------------CTCACCATG`	`52 bp deleted → frameshift`
20.8	`GGACTCGGCGCGC-----------------------------------------------------------TCACCATG`	`59 bp deleted → frameshift`
20.0	`GGACTCGGC------------------------------------------------------------AGCTCACCATG`	`60 bp deleted → no frameshift`
18.8	`GGACTCGGCGC-------------------------------------------------------------TCACCATG`	`61 bp deleted → frameshift`
18.3	`GGACT--------------------------------------------------------ATTCTCGCAGCTCACCATG`	`56 bp deleted → frameshift`
17.2	`GGACTCGGCGCGCC---------------------------------------------------------------ATG`	`63 bp deleted → no frameshift`
17.2	`GGACTCGGC---------------------------------------------------------------TCACCATG`	`63 bp deleted → no frameshift`
16.5	`GGACTC--------------------------------------------------------------------ACCATG`	`68 bp deleted → frameshift`
15.6	`GGACTCGGCGCGCCGGAAGTG-----------------------------------------------------------`	`59 bp deleted → frameshift`
15.6	`GGACTC-----------------------------------------------------------TCGCAGCTCACCATG`	`59 bp deleted → frameshift`
8.1	`GGAC------------------------------------------------------------------------CATG`	`72 bp deleted → no frameshift`

Version 4.7 - Documentation - Contact us - Downloads/local installation - Paper - License