CRISPOR

Micro-homology scoring of potential deletions

Guide: GGCCGTCTTCCCCTCCATCG TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	(Wild-type sequence)
325.6	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`18 bp deleted → no frameshift`
282.0	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCG-------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`7 bp deleted → frameshift`
256.2	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC-----------------CAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`17 bp deleted → frameshift`
253.6	`GCGGGCGACGATGCCCCCCGGGC-----------------------GCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`23 bp deleted → frameshift`
236.0	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCA---------------GGCACCAGGTAGGGGAGCTGGCTGGGTG`	`15 bp deleted → no frameshift`
232.2	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCC-------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`19 bp deleted → frameshift`
208.8	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCC-------------CCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`13 bp deleted → frameshift`
199.8	`GCGGGCGACGATGCCCCCCGGG----------------------GCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`22 bp deleted → frameshift`
198.8	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCC--------------CAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`14 bp deleted → frameshift`
183.0	`GCGGGCGACGATGCCCC----------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`34 bp deleted → frameshift`
179.6	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC----------------CCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`16 bp deleted → frameshift`
172.2	`GCGGGCGACGATGCCCCCCGGGCC-------------------------CCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`25 bp deleted → frameshift`
166.5	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCA----------------------GGTAGGGGAGCTGGCTGGGTG`	`22 bp deleted → frameshift`
161.6	`GCGGGCGACGATGCCCCCC--------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`32 bp deleted → frameshift`
153.6	`GCGGGCGACGATGCCCCC---------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`33 bp deleted → no frameshift`
147.2	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCC--------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`20 bp deleted → frameshift`
140.0	`GCGGGCGACGATGCCCCCCGGG---------------------GGCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`21 bp deleted → no frameshift`
135.0	`GCGGGCG----------------------------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`40 bp deleted → frameshift`
133.2	`GCGGGCGACGATGCCCCCCGGGCCG----------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`22 bp deleted → frameshift`
127.2	`GCGGGCGACGATGCCCCCC-------------------------------CAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`31 bp deleted → frameshift`
121.2	`GCGGGCGACGATGCCCCCCGGGC--------------------------------ACCAGGTAGGGGAGCTGGCTGGGTG`	`32 bp deleted → frameshift`
120.4	`GCGGGCGACGATGCCCCCCGG------------------------CGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`24 bp deleted → no frameshift`
116.1	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCA-------------------CCAGGTAGGGGAGCTGGCTGGGTG`	`19 bp deleted → frameshift`
114.8	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCC-------------------------AGGTAGGGGAGCTGGCTGGGTG`	`25 bp deleted → frameshift`
109.2	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCC--------------------------AGGTAGGGGAGCTGGCTGGGTG`	`26 bp deleted → frameshift`
109.2	`GCGGGCGACGATGCCCCCCGGGCC--------------------------CAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`26 bp deleted → frameshift`
104.4	`GCGGGCGACGATGCCC-----------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`35 bp deleted → frameshift`
103.6	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCC---------------------------AGGTAGGGGAGCTGGCTGGGTG`	`27 bp deleted → no frameshift`
103.6	`GCGGGCGACGATGCCCCCCGGGCC---------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`27 bp deleted → no frameshift`
103.6	`GCGGGCGACGATGCCCCCCG---------------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`27 bp deleted → no frameshift`
89.2	`GCGGGCGACGATGCCCCCC------------------------------CCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`30 bp deleted → no frameshift`
85.2	`GCGGG---------------------------------------GCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`39 bp deleted → no frameshift`
80.8	`GCGGGCGACGATGC--------------------------------GCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`32 bp deleted → frameshift`
76.8	`GCGGGCGACGATGCCCCCCGG---------------------------------CACCAGGTAGGGGAGCTGGCTGGGTG`	`33 bp deleted → no frameshift`
73.2	`GCGGGCGACGATGCCCCCCGGGCC----------------------------------AGGTAGGGGAGCTGGCTGGGTG`	`34 bp deleted → frameshift`
70.5	`GCGGGCGACGATG-----------------------------GGGCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`29 bp deleted → frameshift`
66.6	`GCGGGCGACGATGCCCCCCGGG--------------------------------------------GAGCTGGCTGGGTG`	`44 bp deleted → frameshift`
66.6	`GCG--------------------------------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`44 bp deleted → frameshift`
66.0	`GCGGGCGACGATGCC------------------------------------AGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`36 bp deleted → no frameshift`
63.0	`GCGGGCGACGATGCCCCCCGGG---------------------------------------------AGCTGGCTGGGTG`	`45 bp deleted → no frameshift`
62.8	`GCGGGCGACG-------------------------------------CCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`37 bp deleted → frameshift`
60.0	`GCGGG--------------------------------------GGCGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`38 bp deleted → frameshift`
56.8	`GCGGGCGACGATGCCCCCCGGG---------------------------------------TAGGGGAGCTGGCTGGGTG`	`39 bp deleted → no frameshift`
56.8	`GCGGGCGACGATGCCCCCC---------------------------------------AGGTAGGGGAGCTGGCTGGGTG`	`39 bp deleted → no frameshift`
54.0	`GCGGGCGACGATGCCCCCCGG----------------------------------------TAGGGGAGCTGGCTGGGTG`	`40 bp deleted → frameshift`
54.0	`GCGGGCGACGATGCCCCC----------------------------------------AGGTAGGGGAGCTGGCTGGGTG`	`40 bp deleted → frameshift`
51.6	`GCGGGCGACGATGCCCC-----------------------------------------AGGTAGGGGAGCTGGCTGGGTG`	`41 bp deleted → frameshift`
51.6	`GCGGGCGACGATGC-----------------------------------------ACCAGGTAGGGGAGCTGGCTGGGTG`	`41 bp deleted → frameshift`
51.6	`GCGG-----------------------------------------CGCCCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`41 bp deleted → frameshift`
51.6	`GCGGGC-------------------------------------------------ACCAGGTAGGGGAGCTGGCTGGGTG`	`49 bp deleted → frameshift`
49.5	`GCGGGCGACGATGCCCCCCGGGCCGT------------------------------------AGGGGAGCTGGCTGGGTG`	`36 bp deleted → no frameshift`
48.8	`GCGGGCGACGATGCCC------------------------------------------AGGTAGGGGAGCTGGCTGGGTG`	`42 bp deleted → no frameshift`
48.8	`GCGGGC------------------------------------------CCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`42 bp deleted → no frameshift`
47.1	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCT-------------------------------------GGCTGGGTG`	`37 bp deleted → frameshift`
46.8	`GCGGGCGACGATGCCCCCCGGGC---------------------------------------------------TGGGTG`	`51 bp deleted → no frameshift`
46.4	`GCGGGCGACGATGCCCCCCGGG-------------------------------------------GGAGCTGGCTGGGTG`	`43 bp deleted → frameshift`
46.4	`GCGGGCGACGATGCC-------------------------------------------AGGTAGGGGAGCTGGCTGGGTG`	`43 bp deleted → frameshift`
40.0	`GCGGGCGACGATGCCCCCCGG----------------------------------------------AGCTGGCTGGGTG`	`46 bp deleted → frameshift`
40.0	`GC----------------------------------------------CCCAGGCACCAGGTAGGGGAGCTGGCTGGGTG`	`46 bp deleted → frameshift`
38.7	`GCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCT-----------------------------------------GGGTG`	`41 bp deleted → frameshift`
38.0	`GCGGGCGACGATGCCCCCCGGGC-----------------------------------------------TGGCTGGGTG`	`47 bp deleted → frameshift`
36.6	`GCGGGCGACGATGCCCCCCGGG--------------------------------------------------------TG`	`56 bp deleted → frameshift`
34.8	`GCGGGCGACGATGCCCCCCGGGCCGTCT-------------------------------------------GGCTGGGTG`	`43 bp deleted → frameshift`
32.8	`GCGG--------------------------------------------------CACCAGGTAGGGGAGCTGGCTGGGTG`	`50 bp deleted → frameshift`
29.6	`GCGGGCGACGATGCCCCCCGG----------------------------------------------------CTGGGTG`	`52 bp deleted → frameshift`
28.5	`GCGGGCGACGATGCCCCCCGGGCCGTCT-----------------------------------------------GGGTG`	`47 bp deleted → frameshift`
28.4	`GC-----------------------------------------------------ACCAGGTAGGGGAGCTGGCTGGGTG`	`53 bp deleted → frameshift`
28.2	`GCGGG-------------------------------------------------------------GAGCTGGCTGGGTG`	`61 bp deleted → frameshift`
27.3	`GCGGGCGAC------------------------------------------------CAGGTAGGGGAGCTGGCTGGGTG`	`48 bp deleted → no frameshift`
27.0	`GCGGG--------------------------------------------------------------AGCTGGCTGGGTG`	`62 bp deleted → frameshift`
25.6	`GCGGGCGACGATGCCCCCCGGG-------------------------------------------------------GTG`	`55 bp deleted → frameshift`
24.4	`GCGGGCGACGATGC--------------------------------------------------------TGGCTGGGTG`	`56 bp deleted → frameshift`
24.4	`GCGGG--------------------------------------------------------TAGGGGAGCTGGCTGGGTG`	`56 bp deleted → frameshift`
23.2	`GCGGGCGACGATGCCCCCCGG---------------------------------------------------------TG`	`57 bp deleted → no frameshift`
23.2	`GCGG---------------------------------------------------------TAGGGGAGCTGGCTGGGTG`	`57 bp deleted → no frameshift`
21.3	`GCGGGCGACGATGCCCCCCGGGCCGT-----------------------------------------------------G`	`53 bp deleted → frameshift`
20.0	`GCGGGCGACGATGC------------------------------------------------------------TGGGTG`	`60 bp deleted → no frameshift`
20.0	`GCGGG------------------------------------------------------------GGAGCTGGCTGGGTG`	`60 bp deleted → no frameshift`
19.8	`GCGGGC--------------------------------------------------------------------TGGGTG`	`68 bp deleted → frameshift`
17.4	`GCGGGCGACGA---------------------------------------------------------GCTGGCTGGGTG`	`57 bp deleted → no frameshift`
17.2	`GCGG---------------------------------------------------------------AGCTGGCTGGGTG`	`63 bp deleted → no frameshift`
16.4	`GCGGGC----------------------------------------------------------------TGGCTGGGTG`	`64 bp deleted → frameshift`
15.6	`GCGGGCGACGATG-----------------------------------------------------------GCTGGGTG`	`59 bp deleted → frameshift`
15.6	`GCGGG-------------------------------------------------------------------------TG`	`73 bp deleted → frameshift`
15.0	`GCGGGCGA------------------------------------------------------------GCTGGCTGGGTG`	`60 bp deleted → no frameshift`
13.2	`GC--------------------------------------------------------------------TGGCTGGGTG`	`68 bp deleted → frameshift`
12.9	`GCGGGCGACGATG---------------------------------------------------------------GGTG`	`63 bp deleted → no frameshift`
12.8	`GCGG---------------------------------------------------------------------CTGGGTG`	`69 bp deleted → no frameshift`
10.8	`GCGGG------------------------------------------------------------------------GTG`	`72 bp deleted → no frameshift`
10.8	`GC------------------------------------------------------------------------TGGGTG`	`72 bp deleted → no frameshift`
10.5	`GCGGGCGACGATG-------------------------------------------------------------------`	`67 bp deleted → frameshift`
10.0	`GCGG--------------------------------------------------------------------------TG`	`74 bp deleted → frameshift`

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