CRISPOR

Micro-homology scoring of potential deletions

Guide: TCGCGGGCGACGATGCCCCC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	(Wild-type sequence)
427.0	`CGGCATGTGCAAGGCCGGCTTCGCGGGC-----------------CGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`17 bp deleted → frameshift`
382.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCC---------GTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`9 bp deleted → no frameshift`
377.6	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC---------------TCCATCGTGGGGCGCCCCAGGCACC`	`15 bp deleted → no frameshift`
359.2	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCC----------------TCCATCGTGGGGCGCCCCAGGCACC`	`16 bp deleted → frameshift`
298.2	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC--------------CTCCATCGTGGGGCGCCCCAGGCACC`	`14 bp deleted → frameshift`
296.4	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC------GTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`6 bp deleted → no frameshift`
282.0	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCC-------GTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`7 bp deleted → frameshift`
268.0	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCC--------GTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`8 bp deleted → frameshift`
256.2	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCC-----------------TCCATCGTGGGGCGCCCCAGGCACC`	`17 bp deleted → frameshift`
242.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACG----------GGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`10 bp deleted → frameshift`
223.0	`CGGCATGTGCAAGGCCG------------------------------TCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`30 bp deleted → no frameshift`
208.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC-------------CCTCCATCGTGGGGCGCCCCAGGCACC`	`13 bp deleted → frameshift`
208.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCG-------------GGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`13 bp deleted → frameshift`
188.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACG---------------TCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`15 bp deleted → no frameshift`
183.0	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCC----------------------------------AGGCACC`	`34 bp deleted → frameshift`
179.6	`CGGCATGTGCAAGGCCGGCTTCGCGGG----------------GCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`16 bp deleted → frameshift`
172.2	`CGGCATGTGCAAGGCCGG-------------------------GCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`25 bp deleted → frameshift`
163.8	`CGGCATGTGCAAGGCCGGC--------------------------CGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`26 bp deleted → frameshift`
162.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC------------------ATCGTGGGGCGCCCCAGGCACC`	`18 bp deleted → no frameshift`
162.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCC------------------TCCATCGTGGGGCGCCCCAGGCACC`	`18 bp deleted → no frameshift`
162.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCG------------------TCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`18 bp deleted → no frameshift`
162.8	`CGGCATGTGCAAGGCCGGCTTCGCGG------------------CCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`18 bp deleted → no frameshift`
154.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCC-------------------ATCGTGGGGCGCCCCAGGCACC`	`19 bp deleted → frameshift`
154.8	`CGGCATGTGCAAGGCCGGCTTCG-------------------GGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`19 bp deleted → frameshift`
153.6	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC---------------------------------AGGCACC`	`33 bp deleted → no frameshift`
147.2	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCC--------------------ATCGTGGGGCGCCCCAGGCACC`	`20 bp deleted → frameshift`
140.0	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCC---------------------ATCGTGGGGCGCCCCAGGCACC`	`21 bp deleted → no frameshift`
140.0	`CGGCATGTGCAAGGCCGGCTTCGC---------------------CGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`21 bp deleted → no frameshift`
135.0	`CGGCATGTGCAAGGCCGGCTTCGCGGGCG----------------------------------------CCCCAGGCACC`	`40 bp deleted → frameshift`
133.8	`CGGCATGTGCAAGGCCGGCTTC------------------------------CCCTCCATCGTGGGGCGCCCCAGGCACC`	`30 bp deleted → no frameshift`
133.2	`CGGCATGTGCAAGGCCGGCTTCGCG----------------------TCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`22 bp deleted → frameshift`
121.2	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC--------------------------------CAGGCACC`	`32 bp deleted → frameshift`
120.4	`CGGCATGTGCAAGGCCGGCTTCG------------------------TCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`24 bp deleted → no frameshift`
104.4	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCC-----------------------------------AGGCACC`	`35 bp deleted → frameshift`
94.0	`CGGCATGTGCAAGG-----------------------------GCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`29 bp deleted → frameshift`
89.2	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACG------------------------------TGGGGCGCCCCAGGCACC`	`30 bp deleted → no frameshift`
85.2	`CGGCATGTGCAAGGCCGGCTTCGCGGG---------------------------------------GCGCCCCAGGCACC`	`39 bp deleted → no frameshift`
84.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC-------------------------------CCAGGCACC`	`31 bp deleted → frameshift`
81.0	`CGG----------------------------------------GCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`40 bp deleted → frameshift`
80.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGC--------------------------------GCCCCAGGCACC`	`32 bp deleted → frameshift`
77.7	`CGGCATGTGCAAGGCCGGCTTC---------------------------TTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`27 bp deleted → no frameshift`
77.4	`CGGC-----------------------------------------CGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`41 bp deleted → frameshift`
76.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCG---------------------------------TGGGGCGCCCCAGGCACC`	`33 bp deleted → no frameshift`
71.0	`CGGCATGTGCAAGGCCGGCTTCG---------------------------------------TGGGGCGCCCCAGGCACC`	`39 bp deleted → no frameshift`
70.5	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATG-----------------------------GGGCGCCCCAGGCACC`	`29 bp deleted → frameshift`
69.6	`CGGCATGTGC-----------------------------------CGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`35 bp deleted → frameshift`
66.6	`CGGCATGTGCAAGGCCGGCTTCGCG--------------------------------------------CCCCAGGCACC`	`44 bp deleted → frameshift`
66.0	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCC------------------------------------AGGCACC`	`36 bp deleted → no frameshift`
62.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACG-------------------------------------CCCCAGGCACC`	`37 bp deleted → frameshift`
62.8	`CGGCATGTGCAAGGCCGGCTTCGCG-------------------------------------TGGGGCGCCCCAGGCACC`	`37 bp deleted → frameshift`
62.8	`CGGCATGTGCAAGGCC-------------------------------------CCTCCATCGTGGGGCGCCCCAGGCACC`	`37 bp deleted → frameshift`
60.0	`CGGCATGTGCAAGGCCGGCTTCGCGGG--------------------------------------GGCGCCCCAGGCACC`	`38 bp deleted → frameshift`
60.0	`CGGCATGTGCAAGGCCGGCTTCGC----------------------------------------------CCCAGGCACC`	`46 bp deleted → frameshift`
60.0	`CGGCATGTGCAAGGCC--------------------------------------CTCCATCGTGGGGCGCCCCAGGCACC`	`38 bp deleted → frameshift`
56.8	`CGGCATGTGCAAGGCC---------------------------------------TCCATCGTGGGGCGCCCCAGGCACC`	`39 bp deleted → no frameshift`
54.6	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGAT--------------------------CGTGGGGCGCCCCAGGCACC`	`26 bp deleted → frameshift`
54.0	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC----------------------------------------`	`40 bp deleted → frameshift`
52.2	`CGGCATGTGCAAGGCCGGCTTC-----------------------------------CATCGTGGGGCGCCCCAGGCACC`	`35 bp deleted → frameshift`
51.6	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCC-----------------------------------------`	`41 bp deleted → frameshift`
51.6	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGC-----------------------------------------ACC`	`41 bp deleted → frameshift`
51.6	`CGGCATGTGCAAGGCCGGCTTCGCGG-----------------------------------------CGCCCCAGGCACC`	`41 bp deleted → frameshift`
51.6	`CGGCATGTGCAAGGCCGGCTTCGCGGGC-------------------------------------------------ACC`	`49 bp deleted → frameshift`
51.6	`CGGCATGTGCAAGGCCGGC-------------------------------------------------GCCCCAGGCACC`	`49 bp deleted → frameshift`
49.5	`CGGCATGTGCAAGGCCGGCT------------------------------------CCATCGTGGGGCGCCCCAGGCACC`	`36 bp deleted → no frameshift`
48.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCC------------------------------------------`	`42 bp deleted → no frameshift`
48.8	`CGGCATGTGCAAGGCCGGCTTCGCGGGC------------------------------------------CCCAGGCACC`	`42 bp deleted → no frameshift`
48.8	`CGGCATGTGCAAGGCC------------------------------------------ATCGTGGGGCGCCCCAGGCACC`	`42 bp deleted → no frameshift`
46.4	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCC-------------------------------------------`	`43 bp deleted → frameshift`
42.6	`CGGCATGTGCAAGGC-----------------------------------------------------GCCCCAGGCACC`	`53 bp deleted → frameshift`
42.0	`CGGCATGTGCAAGGCCG---------------------------------------------TGGGGCGCCCCAGGCACC`	`45 bp deleted → no frameshift`
42.0	`CG---------------------------------------------TCTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`45 bp deleted → no frameshift`
40.5	`CGGCATGT----------------------------------------CTTCCCCTCCATCGTGGGGCGCCCCAGGCACC`	`40 bp deleted → frameshift`
38.4	`CGGCATGTGCAAGGCC-------------------------------------------------------CCAGGCACC`	`55 bp deleted → frameshift`
38.0	`CGGCATGTGCAAGGCCGG-----------------------------------------------GGCGCCCCAGGCACC`	`47 bp deleted → frameshift`
36.4	`CGGCATGTGCAAGGCCGG------------------------------------------------GCGCCCCAGGCACC`	`48 bp deleted → no frameshift`
33.0	`CGGCATGTGCAAGGCCGGC----------------------------------------------------------ACC`	`58 bp deleted → frameshift`
32.8	`CGGCATGTGCAAGGCCGGCTTCGCGG--------------------------------------------------CACC`	`50 bp deleted → frameshift`
32.0	`CGGCATGTG-------------------------------------------------------GGGCGCCCCAGGCACC`	`55 bp deleted → frameshift`
31.5	`CGGCATGTGCAAGGC--------------------------------------------------------------ACC`	`62 bp deleted → frameshift`
31.2	`CGGCATGTGCAAGGCCGGC---------------------------------------------------CCCAGGCACC`	`51 bp deleted → no frameshift`
31.2	`CGGCATGTGCAAGG---------------------------------------------------GGCGCCCCAGGCACC`	`51 bp deleted → no frameshift`
29.6	`CGGCATGTGCAAGGCCG----------------------------------------------------CCCCAGGCACC`	`52 bp deleted → frameshift`
29.6	`CGGCATGTGCAAGG----------------------------------------------------GCGCCCCAGGCACC`	`52 bp deleted → frameshift`
28.4	`CGGCATGTGCAAGGCCGGCTTCGC-----------------------------------------------------ACC`	`53 bp deleted → frameshift`
27.3	`CGGCATGTGCAAGGCCGGCTTCGCGGGCGAC------------------------------------------------C`	`48 bp deleted → no frameshift`
27.3	`CGGCATGTGCA------------------------------------------------TCGTGGGGCGCCCCAGGCACC`	`48 bp deleted → no frameshift`
26.8	`CGGCAT------------------------------------------------------CGTGGGGCGCCCCAGGCACC`	`54 bp deleted → no frameshift`
24.6	`CGGC----------------------------------------------------------------GCCCCAGGCACC`	`64 bp deleted → frameshift`
24.4	`CGGCATGTGCAAGGCC--------------------------------------------------------CAGGCACC`	`56 bp deleted → frameshift`
23.2	`CGGCATGTGCAAGGCC---------------------------------------------------------AGGCACC`	`57 bp deleted → no frameshift`
22.0	`CGGCATGTGC----------------------------------------------------------GCCCCAGGCACC`	`58 bp deleted → frameshift`
20.0	`CGGCATGTGC------------------------------------------------------------CCCAGGCACC`	`60 bp deleted → no frameshift`
20.0	`CG------------------------------------------------------------TGGGGCGCCCCAGGCACC`	`60 bp deleted → no frameshift`
18.2	`CGGCA-------------------------------------------------------------------------CC`	`73 bp deleted → frameshift`
18.0	`CGG--------------------------------------------------------------GGCGCCCCAGGCACC`	`62 bp deleted → frameshift`
17.5	`CGGCATGTGCA-------------------------------------------------------------------CC`	`67 bp deleted → frameshift`
17.4	`CGGCATG---------------------------------------------------------GGGCGCCCCAGGCACC`	`57 bp deleted → no frameshift`
17.2	`CGG---------------------------------------------------------------GCGCCCCAGGCACC`	`63 bp deleted → no frameshift`
16.4	`CGGCATGTGCAAGGCC----------------------------------------------------------------`	`64 bp deleted → frameshift`
14.8	`CGGC------------------------------------------------------------------CCCAGGCACC`	`66 bp deleted → no frameshift`
14.0	`CG-------------------------------------------------------------------CCCCAGGCACC`	`67 bp deleted → frameshift`
12.9	`CGGCATGTGCA---------------------------------------------------------------GGCACC`	`63 bp deleted → no frameshift`
9.6	`CGGCA---------------------------------------------------------------------GGCACC`	`69 bp deleted → no frameshift`

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