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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529653_F | TCGTCGGCAGCGTCGAGCGCGGCGATATCATCAT | 60.8 | 1.00 |
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529653_R | GTCTCGTGGGCTCGGCTGGCGGCCTAAGGACTC | 59.4 | 1.00 |
| mm4__chr14 61.99 Mbp_chr14_61989610_F | Primer3: not found at this Tm | N.d. | 0.72 |
| mm4__chr14 61.99 Mbp_chr14_61989610_R | Primer3: not found at this Tm | N.d. | 0.72 |
| mm4__chr1 213.43 Mbp_chr1_213425015_F | TCGTCGGCAGCGTCATAGCCAGCCACTTTGTCCC | 60.0 | 0.49 |
| mm4__chr1 213.43 Mbp_chr1_213425015_R | GTCTCGTGGGCTCGGGGATGAGGGGTCTTGCTTCC | 60.1 | 0.49 |
| mm3__chr5 51.00 Mbp_chr5_50995167_F | TCGTCGGCAGCGTCCCACAAATGCATTGCAGCCA | 60.3 | 0.47 |
| mm3__chr5 51.00 Mbp_chr5_50995167_R | GTCTCGTGGGCTCGGTCACCAAGGAATGTGCCCTC | 59.9 | 0.47 |
| mm4__chrX 105.24 Mbp_chrX_105235253_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4__chrX 105.24 Mbp_chrX_105235253_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4__chr2 241.72 Mbp_chr2_241717933_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4__chr2 241.72 Mbp_chr2_241717933_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4__chrX 115.44 Mbp_chrX_115439028_F | TCGTCGGCAGCGTCGGCAGACAAGGGAAGAGGTG | 60.3 | 0.31 |
| mm4__chrX 115.44 Mbp_chrX_115439028_R | GTCTCGTGGGCTCGGATTCGGGCATTCGACGATGA | 59.8 | 0.31 |
| mm4__chr1 8.84 Mbp_chr1_8843914_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4__chr1 8.84 Mbp_chr1_8843914_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4__chr18 56.54 Mbp_chr18_56542707_F | TCGTCGGCAGCGTCTTGGCCAACCCTGGAACTAC | 59.8 | 0.29 |
| mm4__chr18 56.54 Mbp_chr18_56542707_R | GTCTCGTGGGCTCGGAGGCAAAGTCTGTGTCACCT | 59.1 | 0.29 |
| mm4__chr18 67.53 Mbp_chr18_67528174_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4__chr18 67.53 Mbp_chr18_67528174_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4__chr1 220.48 Mbp_chr1_220478879_F | TCGTCGGCAGCGTCACTCAATTTTACTGCTGGACATTT | 57.3 | 0.19 |
| mm4__chr1 220.48 Mbp_chr1_220478879_R | GTCTCGTGGGCTCGGCAGCCCCACTACTTCCACAG | 60.0 | 0.19 |
| mm3__chr9 26.43 Mbp_chr9_26425164_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3__chr9 26.43 Mbp_chr9_26425164_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4__chr13 62.59 Mbp_chr13_62591939_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4__chr13 62.59 Mbp_chr13_62591939_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4__chr2 41.83 Mbp_chr2_41827166_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4__chr2 41.83 Mbp_chr2_41827166_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4__chr9 95.08 Mbp_chr9_95081224_F | TCGTCGGCAGCGTCCGGGTTTCCTTCCTGTGAGA | 59.3 | 0.14 |
| mm4__chr9 95.08 Mbp_chr9_95081224_R | GTCTCGTGGGCTCGGGACACACTGCGGACATCAGA | 60.0 | 0.14 |
| mm2__chr15 49.86 Mbp_chr15_49856994_F | TCGTCGGCAGCGTCACCCAGGTAAAATGCCACCA | 59.5 | 0.13 |
| mm2__chr15 49.86 Mbp_chr15_49856994_R | GTCTCGTGGGCTCGGGACTAGCCCCTGATGCACTG | 60.1 | 0.13 |
| mm4__chr3 128.50 Mbp_chr3_128497418_F | TCGTCGGCAGCGTCCTGTGTGTGTCCTGCCGTAT | 60.0 | 0.12 |
| mm4__chr3 128.50 Mbp_chr3_128497418_R | GTCTCGTGGGCTCGGACACCATTCCCTGTCGCTTC | 60.3 | 0.12 |
| mm4__chr20 35.38 Mbp_chr20_35375544_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr20 35.38 Mbp_chr20_35375544_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm3__chr12 120.74 Mbp_chr12_120742932_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3__chr12 120.74 Mbp_chr12_120742932_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr12 128.27 Mbp_chr12_128268032_F | TCGTCGGCAGCGTCCCGGTTCTTTGCATCCCCTT | 60.6 | 0.11 |
| mm4__chr12 128.27 Mbp_chr12_128268032_R | GTCTCGTGGGCTCGGCAGAGAGCCCCTGGTTTCTG | 60.0 | 0.11 |
| mm4__chr16 47.12 Mbp_chr16_47121263_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr16 47.12 Mbp_chr16_47121263_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr8 136.35 Mbp_chr8_136353577_F | TCGTCGGCAGCGTCAGTACTCACTGAGGGATGGAT | 57.3 | 0.10 |
| mm4__chr8 136.35 Mbp_chr8_136353577_R | GTCTCGTGGGCTCGGTCTCAGAGGCAGGCTGGTTA | 60.2 | 0.10 |
| mm4__chr7 33.30 Mbp_chr7_33296579_F | TCGTCGGCAGCGTCTGAGACCTCTGACCCAAACTTG | 59.8 | 0.10 |
| mm4__chr7 33.30 Mbp_chr7_33296579_R | GTCTCGTGGGCTCGGTCTGGGAAGTCAAAGGTTGGT | 59.1 | 0.10 |
| mm4__chr8 78.86 Mbp_chr8_78859202_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr8 78.86 Mbp_chr8_78859202_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3__chr17 17.40 Mbp_chr17_17398267_F | TCGTCGGCAGCGTCCAGATTCACCTCGGCTTCCC | 60.4 | 0.10 |
| mm3__chr17 17.40 Mbp_chr17_17398267_R | GTCTCGTGGGCTCGGTGCGGCCTCACAATGATGAT | 60.1 | 0.10 |
| mm4__chr5 41.06 Mbp_chr5_41060622_F | TCGTCGGCAGCGTCGTAGGGAATGCACCTTCGCT | 60.1 | 0.10 |
| mm4__chr5 41.06 Mbp_chr5_41060622_R | GTCTCGTGGGCTCGGGCACAGAGAGGGAAGCACTT | 59.9 | 0.10 |
| mm4__chr12 107.16 Mbp_chr12_107161219_F | TCGTCGGCAGCGTCGCTCTATGCTGAGTCATCTCCC | 60.0 | 0.10 |
| mm4__chr12 107.16 Mbp_chr12_107161219_R | GTCTCGTGGGCTCGGAGGCTAAGAGGAGAAACTGGA | 57.8 | 0.10 |
| mm3__chr13 45.55 Mbp_chr13_45554787_F | TCGTCGGCAGCGTCGACTGTGTCTCTGGCCTGTC | 60.0 | 0.09 |
| mm3__chr13 45.55 Mbp_chr13_45554787_R | GTCTCGTGGGCTCGGTGGTGTTCAGGCCAAATCCA | 59.8 | 0.09 |
| mm4__chr17 47.49 Mbp_chr17_47492289_F | TCGTCGGCAGCGTCCTCTTCCCCGGCCTAGGTAT | 60.1 | 0.09 |
| mm4__chr17 47.49 Mbp_chr17_47492289_R | GTCTCGTGGGCTCGGGGCTACAGGAGGAGGACAGA | 60.0 | 0.09 |
| mm4__chr17 38.30 Mbp_chr17_38297329_F | TCGTCGGCAGCGTCGGGCTACAGGAGAGGACAGA | 60.0 | 0.09 |
| mm4__chr17 38.30 Mbp_chr17_38297329_R | GTCTCGTGGGCTCGGCTCTTCCCCGGCCTAGGTAT | 60.1 | 0.09 |
| mm4__chr1 113.45 Mbp_chr1_113448714_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr1 113.45 Mbp_chr1_113448714_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chrX 151.96 Mbp_chrX_151958743_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chrX 151.96 Mbp_chrX_151958743_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr15 86.72 Mbp_chr15_86719552_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr15 86.72 Mbp_chr15_86719552_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr20 50.59 Mbp_chr20_50593037_F | TCGTCGGCAGCGTCCAGGGCTCCGTGGATATTCC | 59.9 | 0.08 |
| mm4__chr20 50.59 Mbp_chr20_50593037_R | GTCTCGTGGGCTCGGAGCGTCTCTGACCTGGAGAA | 60.2 | 0.08 |
| mm3__chr1 44.61 Mbp_chr1_44609829_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3__chr1 44.61 Mbp_chr1_44609829_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr1 31.76 Mbp_chr1_31756875_F | TCGTCGGCAGCGTCAGGCTGGGGTCATCCTATGT | 60.0 | 0.07 |
| mm4__chr1 31.76 Mbp_chr1_31756875_R | GTCTCGTGGGCTCGGGACCAGGGTCTTAACTTATGAGG | 57.9 | 0.07 |
| mm4__chr6 93.92 Mbp_chr6_93918524_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr6 93.92 Mbp_chr6_93918524_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr8 18.79 Mbp_chr8_18785023_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr8 18.79 Mbp_chr8_18785023_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr7 10.95 Mbp_chr7_10948749_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr7 10.95 Mbp_chr7_10948749_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr4 72.91 Mbp_chr4_72913796_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr4 72.91 Mbp_chr4_72913796_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3__chr6 149.05 Mbp_chr6_149045941_F | TCGTCGGCAGCGTCGTCACCCAAGTCTGGAAGCT | 59.6 | 0.06 |
| mm3__chr6 149.05 Mbp_chr6_149045941_R | GTCTCGTGGGCTCGGCTTCTGTGATGGTTTCCCAGC | 59.1 | 0.06 |
| mm3__chr22 48.04 Mbp_chr22_48036209_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3__chr22 48.04 Mbp_chr22_48036209_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr4 145.71 Mbp_chr4_145709857_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr4 145.71 Mbp_chr4_145709857_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr2 64.61 Mbp_chr2_64605710_F | TCGTCGGCAGCGTCTGTCTGGCAGCTTTTCGGAT | 59.9 | 0.05 |
| mm4__chr2 64.61 Mbp_chr2_64605710_R | GTCTCGTGGGCTCGGCTCCTTCAATCGGGCCTTGT | 60.0 | 0.05 |
| mm4__chr5 113.06 Mbp_chr5_113061208_F | TCGTCGGCAGCGTCAGGTACCTAGAGGCTCACGG | 60.1 | 0.04 |
| mm4__chr5 113.06 Mbp_chr5_113061208_R | GTCTCGTGGGCTCGGGAGTCCCTTGTCTCAGCACC | 60.0 | 0.04 |
| mm3__chr17 17.01 Mbp_chr17_17010185_F | TCGTCGGCAGCGTCGTCCAAATTGCAACAGGGGC | 60.3 | 0.04 |
| mm3__chr17 17.01 Mbp_chr17_17010185_R | GTCTCGTGGGCTCGGAGGCCTTCTGAACCACAGTG | 59.8 | 0.04 |
| mm3__chr17 16.87 Mbp_chr17_16865930_F | TCGTCGGCAGCGTCAGAACTCTGGCTGGGCTTTG | 60.2 | 0.04 |
| mm3__chr17 16.87 Mbp_chr17_16865930_R | GTCTCGTGGGCTCGGGGCTCCTCCTGTCAGCATTT | 60.0 | 0.04 |
| mm4__chr19 16.62 Mbp_chr19_16616902_F | TCGTCGGCAGCGTCGAACCAGGTAGGCAGACACC | 60.0 | 0.04 |
| mm4__chr19 16.62 Mbp_chr19_16616902_R | GTCTCGTGGGCTCGGTCACGCCAGGTTGTCTTCTC | 59.9 | 0.04 |
| mm4__chr6 41.41 Mbp_chr6_41408001_F | TCGTCGGCAGCGTCAGGTGATCCGTCTCCTCCTT | 59.6 | 0.03 |
| mm4__chr6 41.41 Mbp_chr6_41408001_R | GTCTCGTGGGCTCGGGACAGTCCCTGACACTGGTG | 59.9 | 0.03 |
| mm4__chr1 180.26 Mbp_chr1_180259844_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr1 180.26 Mbp_chr1_180259844_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr3 133.62 Mbp_chr3_133615525_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr3 133.62 Mbp_chr3_133615525_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chrX 6.38 Mbp_chrX_6382310_F | TCGTCGGCAGCGTCTTTCTGTCCTTCTGAGGCCC | 59.3 | 0.03 |
| mm4__chrX 6.38 Mbp_chrX_6382310_R | GTCTCGTGGGCTCGGAGGGATGGTCTAAGCAAAGGT | 58.7 | 0.03 |
| mm3__chr9 104.89 Mbp_chr9_104894747_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3__chr9 104.89 Mbp_chr9_104894747_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr2 196.29 Mbp_chr2_196288495_F | TCGTCGGCAGCGTCCAAACAGCCCAGGTCAGACT | 59.8 | 0.03 |
| mm4__chr2 196.29 Mbp_chr2_196288495_R | GTCTCGTGGGCTCGGGAACGGCTGGTCTCCCTTAA | 59.3 | 0.03 |
| mm4__chr10 80.05 Mbp_chr10_80045200_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr10 80.05 Mbp_chr10_80045200_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr10 80.03 Mbp_chr10_80029443_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr10 80.03 Mbp_chr10_80029443_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr5 68.65 Mbp_chr5_68648685_F | TCGTCGGCAGCGTCAAGAAGCCCACAGCCACATG | 60.8 | 0.03 |
| mm4__chr5 68.65 Mbp_chr5_68648685_R | GTCTCGTGGGCTCGGACAAGGCCCAGTCCTGTTTC | 60.1 | 0.03 |
| mm4__chr17 77.77 Mbp_chr17_77771379_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr17 77.77 Mbp_chr17_77771379_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr5 134.59 Mbp_chr5_134591276_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr5 134.59 Mbp_chr5_134591276_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr6 117.99 Mbp_chr6_117986131_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr6 117.99 Mbp_chr6_117986131_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3__chr1 12.67 Mbp_chr1_12666529_F | TCGTCGGCAGCGTCTGAGGTCATCGCTCAGCTTC | 59.8 | 0.02 |
| mm3__chr1 12.67 Mbp_chr1_12666529_R | GTCTCGTGGGCTCGGGGTCCTCCAAGCGCTTCTTA | 59.7 | 0.02 |
| mm4__chrX 154.28 Mbp_chrX_154275700_F | TCGTCGGCAGCGTCCATGTGGCCAGGATGGATGA | 59.8 | 0.02 |
| mm4__chrX 154.28 Mbp_chrX_154275700_R | GTCTCGTGGGCTCGGTCCTCCTGCTTTCGCTTCTG | 60.0 | 0.02 |
| mm4__chrX 154.24 Mbp_chrX_154237897_F | TCGTCGGCAGCGTCCATGTGGCCAGGATGGATGA | 59.8 | 0.02 |
| mm4__chrX 154.24 Mbp_chrX_154237897_R | GTCTCGTGGGCTCGGTCCTCCTGCTTTCGCTTCTG | 60.0 | 0.02 |
| mm4__chrX 154.20 Mbp_chrX_154200756_F | TCGTCGGCAGCGTCCATGTGGCCAGGATGGATGA | 59.8 | 0.02 |
| mm4__chrX 154.20 Mbp_chrX_154200756_R | GTCTCGTGGGCTCGGTCCTCCTGCTTTCGCTTCTG | 60.0 | 0.02 |
| mm4__chrX 154.16 Mbp_chrX_154163642_F | TCGTCGGCAGCGTCCATGTGGCCAGGATGGATGA | 59.8 | 0.02 |
| mm4__chrX 154.16 Mbp_chrX_154163642_R | GTCTCGTGGGCTCGGTCCTCCTGCTTTCGCTTCTG | 60.0 | 0.02 |
| mm3__chrX 93.58 Mbp_chrX_93581128_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3__chrX 93.58 Mbp_chrX_93581128_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3__chr16 18.61 Mbp_chr16_18607712_F | TCGTCGGCAGCGTCCTCCAAGCATCCAGGCATGA | 60.1 | 0.01 |
| mm3__chr16 18.61 Mbp_chr16_18607712_R | GTCTCGTGGGCTCGGCAAGAAGTTGGTGCCTGCAC | 59.9 | 0.01 |
| mm3__chr14 23.39 Mbp_chr14_23389454_F | TCGTCGGCAGCGTCCTCCTCCAGCTGCCTTTTGA | 59.9 | 0.01 |
| mm3__chr14 23.39 Mbp_chr14_23389454_R | GTCTCGTGGGCTCGGCCCACAAGCCCCTCATTTCA | 60.2 | 0.01 |
| mm4__chr10 97.71 Mbp_chr10_97714073_F | TCGTCGGCAGCGTCTCACTATCGCCACCAGCAAG | 60.1 | 0.01 |
| mm4__chr10 97.71 Mbp_chr10_97714073_R | GTCTCGTGGGCTCGGCAGCGTGAGGAAGTAGAGGC | 60.1 | 0.01 |
| mm4__chr11 3.42 Mbp_chr11_3424290_F | TCGTCGGCAGCGTCTCTTTCAGATGAGAGGCACTTGA | 59.4 | 0.01 |
| mm4__chr11 3.42 Mbp_chr11_3424290_R | GTCTCGTGGGCTCGGGGTTCTGGTGGGGAACAACA | 60.1 | 0.01 |
| mm3__chr16 16.75 Mbp_chr16_16751497_F | TCGTCGGCAGCGTCTCAGCTGAGTGCAGTCAGTG | 59.9 | 0.01 |
| mm3__chr16 16.75 Mbp_chr16_16751497_R | GTCTCGTGGGCTCGGCTCCAAGCATCCAGGCATGA | 60.1 | 0.01 |
| mm4__chr9 126.20 Mbp_chr9_126197643_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr9 126.20 Mbp_chr9_126197643_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr10 14.53 Mbp_chr10_14532908_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr10 14.53 Mbp_chr10_14532908_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr12 125.84 Mbp_chr12_125843600_F | TCGTCGGCAGCGTCTATTCCAGGGCCAGACTCAC | 58.7 | 0.00 |
| mm4__chr12 125.84 Mbp_chr12_125843600_R | GTCTCGTGGGCTCGGGCAGAAATAATGCTGGTGTGC | 58.4 | 0.00 |
| mm4__chr16 57.00 Mbp_chr16_57003160_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr16 57.00 Mbp_chr16_57003160_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr12 71.62 Mbp_chr12_71616190_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr12 71.62 Mbp_chr12_71616190_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr10 30.00 Mbp_chr10_29997810_F | TCGTCGGCAGCGTCTAACGCTGCAACAAGACCCT | 59.8 | 0.00 |
| mm4__chr10 30.00 Mbp_chr10_29997810_R | GTCTCGTGGGCTCGGTGGATGCGCACCCTAAACAT | 60.0 | 0.00 |
| mm4__chr8 144.53 Mbp_chr8_144526865_F | TCGTCGGCAGCGTCCGAGAGCCGGTTGATTTTCG | 59.6 | 0.00 |
| mm4__chr8 144.53 Mbp_chr8_144526865_R | GTCTCGTGGGCTCGGGGACCATCCCGGCTCTGA | 60.7 | 0.00 |
| mm4__chr6 68.06 Mbp_chr6_68059426_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr6 68.06 Mbp_chr6_68059426_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 98.79 Mbp_chr5_98794453_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 98.79 Mbp_chr5_98794453_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 116.46 Mbp_chr1_116463782_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 116.46 Mbp_chr1_116463782_R | Primer3: not found at this Tm | N.d. | 0.00 |
Primers underlined, off-targets in bold.
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529653 |
GAGCGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGC CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG |
| mm4__chr1 213.43 Mbp_chr1_213425015 |
ATAGCCAGCCACTTTGTCCCTTCCTGCCCCAAGCTCACATCGCTGTTCCAGCAACTCACCAGGGCCACGGAGGAGCCGCC ATGTAGCAGGGAAGCAAGACCCCTCATCC |
| mm3__chr5 51.00 Mbp_chr5_50995167 |
CCACAAATGCATTGCAGCCAATGCCTAAATCCCAAACCCACCTTGGTGAGCTGCAAGTACAGCTGGAATGCTAGACCATG TGAACGCTAAGGACAAGAACAGGCAACCGAGGGCACATTCCTTGGTGA |
| mm4__chrX 115.44 Mbp_chrX_115439028 |
GGCAGACAAGGGAAGAGGTGAGTCTAAGTCAGCAGAATGGTGGATAACTCTGGGCCTAGATGAGCTGAGAGAATAGACCT TGTATTTCACAAGTCCTGGACTTCATCGTCGAATGCCCGAAT |
| mm4__chr18 56.54 Mbp_chr18_56542707 |
TTGGCCAACCCTGGAACTACATTCTTGCTGATCTGTCAGCTGTGGGAGTTAATTCCTTGCAAACTGAGCAATTTTGTCAG CTCACCACGGTGAGGTGACACAGACTTTGCCT |
| mm4__chr1 220.48 Mbp_chr1_220478879 |
ACTCAATTTTACTGCTGGACATTTACTCAATTAATGAATCTTTCAATAAGATACTGACTATCATAAACTCTTGGAAAGAC TGTGGTGAGCTGAGGGAATAAAAATTTGTTTTCCTTTTTTCTGTGGAAGTAGTGGGGCTG |
| mm4__chr9 95.08 Mbp_chr9_95081224 |
CGGGTTTCCTTCCTGTGAGAGCTTTTCTCACTGTTCACCAGGGACAGCAGTCACCTTCCTAGGAGTTCACAGGCAGTGCG CATGTGGGAGCGGATCTGGGGAGACCTTCATTGGCCGCCTCTGATGTCCGCAGTGTGTC |
| mm2__chr15 49.86 Mbp_chr15_49856994 |
ACCCAGGTAAAATGCCACCACAGGGTTTAGCAGCTCTGTGGTGAGCTGAGAGAAAAGCCAGAGACAGCAGTGCATCAGGG GCTAGTC |
| mm4__chr3 128.50 Mbp_chr3_128497418 |
CTGTGTGTGTCCTGCCGTATCTCTGCCCAGGTGTGCCCTGGTGAGCTGAGAGAGGGGCACGGGGAAGGGCAGTCGTCGGG GCCTGTCCGTGTGCCAGCGTGGGTGGACTCCGGGCTGGGGAAGCGACAGGGAATGGTGT |
| mm4__chr12 128.27 Mbp_chr12_128268032 |
CCGGTTCTTTGCATCCCCTTTCCGCAACCGCTAGGGTCTCTGTGCGGGCCCTAGAGGAACGCTCACTCATCGCGCAGCTC ACCAGGGACCCCAGCTTGGCAGAAACCAGGGGCTCTCTG |
| mm4__chr8 136.35 Mbp_chr8_136353577 | AGTACTCACTGAGGGATGGATGCTAGTGTACCAGCTCACCAGGGGTCCAAAAGAATATAACCAGCCTGCCTCTGAGA |
| mm4__chr7 33.30 Mbp_chr7_33296579 |
TGAGACCTCTGACCCAAACTTGGCAATTCTCTCAGCTAGCCAGGGAAAACACTTGCAGGGTTCATTACTTTCTTCTGTCA TCTTGAGAGATTCTACCCCATTTTTCTCTTCTACTGACCAACCTTTGACTTCCCAGA |
| mm3__chr17 17.40 Mbp_chr17_17398267 |
CAGATTCACCTCGGCTTCCCCCTTGGCACTTGCTATTTTCACAGCTCTCCAAGGCCATTAGTGACAACTGTGCCCTAAGT AGGTCACAGCGATTATCCCCCTTTTAGGGGCAATCATCATCATCATTGTGAGGCCGCA |
| mm4__chr5 41.06 Mbp_chr5_41060622 |
GTAGGGAATGCACCTTCGCTGTGGTGAGCAGCTTCATGGGCTGCAAGGATCAATTCCAATGTCGCAGCTCACCAGGGCAG TTAAGTGCTTCCCTCTCTGTGC |
| mm4__chr12 107.16 Mbp_chr12_107161219 |
GCTCTATGCTGAGTCATCTCCCTTCTCTATTCTCTCAACTCTCCAAGGAAAATTTTGTTTGATTTCCAGTTTCTCCTCTT AGCCT |
| mm3__chr13 45.55 Mbp_chr13_45554787 |
GACTGTGTCTCTGGCCTGTCCTGCAGGCGGGACCCCGAGCTGTGCCAGAGCTGTTCTGGGAGCTCACCATGGCTGCTGTG AGGCCCTGGGTCCTGGCCTGGAGGGGTTGGATTTGGCCTGAACACCA |
| mm4__chr17 47.49 Mbp_chr17_47492289 |
CTCTTCCCCGGCCTAGGTATGACTCGTATTGTCCCAGCTCACCAAGGACAATTGCACAGAGAGTATTTCCCTCGACTCTG TCCTCCTCCTGTAGCC |
| mm4__chr17 38.30 Mbp_chr17_38297329 |
GGGCTACAGGAGAGGACAGAGTCGAGGGAAATACTCTCTGTGCAATTGTCCTTGGTGAGCTGGGACAATACGAGTCATAC CTAGGCCGGGGAAGAG |
| mm4__chr20 50.59 Mbp_chr20_50593037 |
CAGGGCTCCGTGGATATTCCTCTGCTATGACAGCCACCCACCCCAGTCTTACCTTCCTTGGTGAGCTGGGTGAAGTGCTT CTCCAGGTCAGAGACGCT |
| mm4__chr1 31.76 Mbp_chr1_31756875 |
AGGCTGGGGTCATCCTATGTCCCTTGCCCTGTGGAGAGAGACAGTGGTCAGCGGGCCCCCAGCACAGCCAGCATGGGCTC TGAACCTTCTATGGTGAGCTGCGGGAGTGGGCCTCATAAGTTAAGACCCTGGTC |
| mm3__chr6 149.05 Mbp_chr6_149045941 |
GTCACCCAAGTCTGGAAGCTTGAAGTCCTTTCAATTATACCTTAAAAAAAGCCATCTCCCAGAATATCTGCTTAGAAATG TTCTAGATATTTTCCCAGCTCACCATAGAGCTGGGAAACCATCACAGAAG |
| mm4__chr2 64.61 Mbp_chr2_64605710 |
TGTCTGGCAGCTTTTCGGATACAGGATCAAAATTGTGTGAAACTCTTGCTATGCCCGCACCTCACCCAGGAGCTCAGGCT CATTTAGGAAACAAGGCCCGATTGAAGGAG |
| mm4__chr5 113.06 Mbp_chr5_113061208 |
AGGTACCTAGAGGCTCACGGCAAGGCTGCAACCACCATTCTCACAGCTCTCCAGGGTCCCCACCTGGTGCTGAGACAAGG GACTC |
| mm3__chr17 17.01 Mbp_chr17_17010185 |
GTCCAAATTGCAACAGGGGCAGGTCCCTGCCAGCTGAGGGAGCTATTCTTCCAGCTCAGCAGGGGGTTGGCCAGTCTTGT CCTCTGGGACAAAGCCCAGCCAGAGTTCTGGGCACTGTGGTTCAGAAGGCCT |
| mm3__chr17 16.87 Mbp_chr17_16865930 |
AGAACTCTGGCTGGGCTTTGTCCCAGAGGACAAGACTGGCCAACCCCCTGCTGAGCTGGAAGAATAGCTCCCTCAGCTGG CAGGGACCTGCCCCTGTTGCAATTTGGACTGAGTAAATGCTGACAGGAGGAGCC |
| mm4__chr19 16.62 Mbp_chr19_16616902 |
GAACCAGGTAGGCAGACACCCCCCCACTGCTAGGCCTTGTCATTCATTCCAAGTGCCCATGGTGAGCACAGAGAACAGCG CCCTAAAGAGAAGACAACCTGGCGTGA |
| mm4__chr6 41.41 Mbp_chr6_41408001 |
AGGTGATCCGTCTCCTCCTTCCTGCCAGGCTCAGAGGCCACCAGCACAGATGGCTAGTCTACCAGCTCCCCAGGGGAGCC TCCTCACTGCAGTCCTCTGCGACTCCACACTGCACCAGTGTCAGGGACTGTC |
| mm4__chrX 6.38 Mbp_chrX_6382310 |
TTTCTGTCCTTCTGAGGCCCCATGACTATGTAGTCCCAAACTATTCTCGCCGATCACAAAGGAACATTCTGTTTAGGGCT ACTTTTACCTTTGCTTAGACCATCCCT |
| mm4__chr2 196.29 Mbp_chr2_196288495 |
CAAACAGCCCAGGTCAGACTACCCTGCAGTGATCAAGAACAACATGGACAGCAAAGAACTGTAACACCAAAAAGAAACCC TGCTGTGAAAATGCCATTCTCTCAGACCACCATGGTTAAGGGAGACCAGCCGTTC |
| mm4__chr5 68.65 Mbp_chr5_68648685 |
AAGAAGCCCACAGCCACATGGGTATTTTCGCAGCTTCCCATGGGGGAAAGGCTGACCCTGGATAGGTAGGTGATACATGA CCTCGGAAGTACATGGTTTCATTGTGGCTGGGGCCTAAAAGAAACAGGACTGGGCCTTGT |
| mm3__chr1 12.67 Mbp_chr1_12666529 |
TGAGGTCATCGCTCAGCTTCCTGAGGCCTTCCTGGTGAGCTGTGAGAATGACATACTCCGTGATGACAGCTTGCTCTATA AGAAGCGCTTGGAGGACC |
| mm4__chrX 154.28 Mbp_chrX_154275700 |
CATGTGGCCAGGATGGATGAGCTCACTCTGAGCAAATGTGCCAAAGCAAGGGGCCCTGGGGAGCTGGGGGAAGAGCCAAC AAAACAGAAGCGAAAGCAGGAGGA |
| mm4__chrX 154.24 Mbp_chrX_154237897 |
CATGTGGCCAGGATGGATGAGCTCACTCTGAGCAAATGTGCCAAAGCAAGGGGCCCTGGGGAGCTGGGGGAAGAGCCAAC AAAACAGAAGCGAAAGCAGGAGGA |
| mm4__chrX 154.20 Mbp_chrX_154200756 |
CATGTGGCCAGGATGGATGAGCTCACTCTGAGCAAATGTGCCAAAGCAAGGGGCCCTGGGGAGCTGGGGGAAGAGCCAAC AAAACAGAAGCGAAAGCAGGAGGA |
| mm4__chrX 154.16 Mbp_chrX_154163642 |
CATGTGGCCAGGATGGATGAGCTCACTCTGAGCAAATGTGCCAAAGCAAGGGGCCCTGGGGAGCTGGGGGAAGAGCCAAC AAAACAGAAGCGAAAGCAGGAGGA |
| mm3__chr16 18.61 Mbp_chr16_18607712 |
CTCCAAGCATCCAGGCATGAATCCCCACAGGGCAAGGCAGCAGAGAGGAGCAACAGTGAGCATGATGGGTTATCTATTCT CCCAGCTCCCCACAGCCCACTGACTGCACTCAGCTGAGTGCAGGCACCAACTTCTTG |
| mm3__chr14 23.39 Mbp_chr14_23389454 |
CTCCTCCAGCTGCCTTTTGAGGTCCTCCATTTGCTGGGTATAAGAGAGCTTCCCCCGGGTCAGCTGCGAGATTAGCGCCT CCTTTTCCTCTAGCTGCCGGGCCAACTCTCCTGGAGGTGAAATGAGGGGCTTGTGGG |
| mm4__chr10 97.71 Mbp_chr10_97714073 |
TCACTATCGCCACCAGCAAGTACCAGGGCCCCGTGCACTTCGCGCTCTTCGTGTCCGTGCTCTTCTGGCTGCTCACCCTG GGCCTCTACTTCCTCACGCTG |
| mm4__chr11 3.42 Mbp_chr11_3424290 |
TCTTTCAGATGAGAGGCACTTGAGCCCTGCTCAGCTGCGAGAATAAGAGAGGGGAATTAATTCTATTTGAATACACGTGT TCTCTCACAGCTGTTGTTCCCCACCAGAACC |
| mm3__chr16 16.75 Mbp_chr16_16751497 |
TCAGCTGAGTGCAGTCAGTGGGCTGTGGGGAGCTGGGAGAATAGGTAACCCATCATGCTCACTGTTGCTCCTCTCTGCTG CCTTGCCCTGTGGGGATTCATGCCTGGATGCTTGGAG |
| mm4__chr12 125.84 Mbp_chr12_125843600 |
TATTCCAGGGCCAGACTCACGGCAATTCTCGCAGCTCTGCTTGGCAACAAAACTAAACAAATGCACACCAGCATTATTTC TGC |
| mm4__chr10 30.00 Mbp_chr10_29997810 |
TAACGCTGCAACAAGACCCTCATGAGGTTTGGGGATCCTGACTTGTAAACGGGGCTCTTCTCGCAGCTGCCTAAGGATGT TTAGGGTGCGCATCCA |
| mm4__chr8 144.53 Mbp_chr8_144526865 |
CGAGAGCCGGTTGATTTTCGGCCGGGCTCAGGGAGGGCGTGGCCTCCGCGTTCCCCATCCCCCTCCAGGACCCACGCCCT TCTCGGAGCGCACCAGGGTCAGAGCCGGGATGGTCC |