CRISPOR

Micro-homology scoring of potential deletions

Guide: GCCGTTGTCGACGACGAGCG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	(Wild-type sequence)
467.4	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCG-----ATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`5 bp deleted → frameshift`
362.0	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCG--GCGATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`2 bp deleted → frameshift`
319.0	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGA---------TATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`9 bp deleted → no frameshift`
296.4	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACG------GCGATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`6 bp deleted → no frameshift`
274.5	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGA------------TATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`12 bp deleted → no frameshift`
255.2	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACG---------GCGATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`9 bp deleted → no frameshift`
236.0	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGA---------------TATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`15 bp deleted → no frameshift`
219.6	`CCGGCCTTGCACATGCCGGAGCCGTTGTCG------------GCGATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`12 bp deleted → no frameshift`
181.3	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGC---------------------------TGCGAGAATAGCCG`	`27 bp deleted → no frameshift`
180.6	`CCGGCCTTGCACATGCCGG------------------------CGATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`24 bp deleted → no frameshift`
162.8	`CCGGCCTTGCACATGCCGGAGCCG------------------GCGATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`18 bp deleted → no frameshift`
140.0	`CCGGCCTTGCACATGCCGGAGCCG---------------------ATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`21 bp deleted → no frameshift`
133.8	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCG------------------------------AGAATAGCCG`	`30 bp deleted → no frameshift`
133.2	`CCGGCCTTGCACATGCCGGAGC----------------------GATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`22 bp deleted → frameshift`
128.1	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGAG----------------------------------AATAGCCG`	`34 bp deleted → frameshift`
113.6	`CCGGC---------------------------------------GATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`39 bp deleted → no frameshift`
105.0	`CCGGCCTTGCACATGCCGGAGCCGTTGTC---------------------ATCATCCATGGTGAGCTGCGAGAATAGCCG`	`21 bp deleted → no frameshift`
103.6	`CCGGCCTTGCACATGCCG---------------------------ATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`27 bp deleted → no frameshift`
98.8	`CCGGCCTTGCACATGC----------------------------GATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`28 bp deleted → frameshift`
90.3	`CCGGCCTTGCACATGCCGGAGCCGTTGTC------------------------ATCCATGGTGAGCTGCGAGAATAGCCG`	`24 bp deleted → no frameshift`
81.9	`CCGGCCTTGCACATGCCGGA--------------------------TATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`26 bp deleted → frameshift`
78.5	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGA-------------------------------------GAATAGCCG`	`37 bp deleted → frameshift`
77.7	`CCGGCCTTGCACATGCCGGAGCCGTTGTC---------------------------CATGGTGAGCTGCGAGAATAGCCG`	`27 bp deleted → no frameshift`
77.7	`CCGGCCTTGCACATGCCGGAGC--------------------------------------------TGCGAGAATAGCCG`	`44 bp deleted → frameshift`
73.2	`CCGGCCTTGCACATGCCGGAGCC----------------------------------ATGGTGAGCTGCGAGAATAGCCG`	`34 bp deleted → frameshift`
73.2	`CCGGCCTTGC----------------------------------GATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`34 bp deleted → frameshift`
71.0	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGC---------------------------------------CG`	`39 bp deleted → no frameshift`
67.5	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGA----------------------------------------GAATAGCCG`	`40 bp deleted → frameshift`
66.9	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGA------------------------------GCTGCGAGAATAGCCG`	`30 bp deleted → no frameshift`
63.0	`CCGGCCTTGCACATG---------------------------------------------GTGAGCTGCGAGAATAGCCG`	`45 bp deleted → no frameshift`
60.0	`CCGGCCTTGCACAT--------------------------------------CATCCATGGTGAGCTGCGAGAATAGCCG`	`38 bp deleted → frameshift`
57.6	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGA---------------------------------GCTGCGAGAATAGCCG`	`33 bp deleted → no frameshift`
57.6	`CCGGCCTTGCACATGCCGGAGCCGTTG---------------------------------GTGAGCTGCGAGAATAGCCG`	`33 bp deleted → no frameshift`
54.9	`CCGGCCTTGCACATGCCGGAGCCGTTGT----------------------------------GAGCTGCGAGAATAGCCG`	`34 bp deleted → frameshift`
54.9	`CCGGCCTTGCACATGCCGGAGCCG--------------------------------------------------------`	`56 bp deleted → frameshift`
54.0	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGAGCG----------------------------------------`	`40 bp deleted → frameshift`
54.0	`CCGGCCTTGCACATGCC----------------------------------------ATGGTGAGCTGCGAGAATAGCCG`	`40 bp deleted → frameshift`
51.6	`CCGGCCTTGCACAT-----------------------------------------CCATGGTGAGCTGCGAGAATAGCCG`	`41 bp deleted → frameshift`
49.5	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACGA------------------------------------ATAGCCG`	`36 bp deleted → no frameshift`
49.5	`CCGGCCTTGCACATGCCGGAGCCGTTG------------------------------------AGCTGCGAGAATAGCCG`	`36 bp deleted → no frameshift`
48.8	`CCGGCCTTGCACATGCCGG------------------------------------------TGAGCTGCGAGAATAGCCG`	`42 bp deleted → no frameshift`
48.8	`CCG------------------------------------------ATATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`42 bp deleted → no frameshift`
47.1	`CCGGCCTTGCACATGCCGGAGCCGT-------------------------------------GAGCTGCGAGAATAGCCG`	`37 bp deleted → frameshift`
44.4	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGACG--------------------------------------------`	`44 bp deleted → frameshift`
42.6	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACGA---------------------------------------ATAGCCG`	`39 bp deleted → no frameshift`
40.5	`CCGGCCTTGCA----------------------------------------TCATCCATGGTGAGCTGCGAGAATAGCCG`	`40 bp deleted → frameshift`
40.0	`CCGGCCTTGCACATGCCGGAGCCG----------------------------------------------AGAATAGCCG`	`46 bp deleted → frameshift`
39.0	`CCGGCCTTGCACATGCCGGAG---------------------------------------------------AATAGCCG`	`51 bp deleted → no frameshift`
38.7	`CCGGCCTTGCACATGCCGGAGCCGTTG-----------------------------------------CGAGAATAGCCG`	`41 bp deleted → frameshift`
38.4	`CCGGCCTTGCACAT---------------------------------ATCATCATCCATGGTGAGCTGCGAGAATAGCCG`	`33 bp deleted → no frameshift`
38.0	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGACG-----------------------------------------------`	`47 bp deleted → frameshift`
38.0	`CCGGCCTTGCACATGCCGGAGC-----------------------------------------------GAGAATAGCCG`	`47 bp deleted → frameshift`
36.6	`CCGGCCTTGCACATGCCGGAGCCGTTGTCGA------------------------------------------ATAGCCG`	`42 bp deleted → no frameshift`
36.0	`CCGGCCTTGCACATGCCG--------------------------------------------------------------`	`62 bp deleted → frameshift`
35.5	`CCGGCCTTGCACATGC-----------------------------------------------------GAGAATAGCCG`	`53 bp deleted → frameshift`
34.8	`CCGGCCTTGCA-------------------------------------------TCCATGGTGAGCTGCGAGAATAGCCG`	`43 bp deleted → frameshift`
34.8	`CCGGCCTTGCACAT-----------------------------------CATCATCCATGGTGAGCTGCGAGAATAGCCG`	`35 bp deleted → frameshift`
32.8	`CCGGCCTTGCACATGCCGGAGCCGTTGTCG--------------------------------------------------`	`50 bp deleted → frameshift`
32.8	`CCGGCCTTGCACATGC--------------------------------------------------TGCGAGAATAGCCG`	`50 bp deleted → frameshift`
31.2	`CCGGCC---------------------------------------------------ATGGTGAGCTGCGAGAATAGCCG`	`51 bp deleted → no frameshift`
29.6	`CCGGCCTTGCACATGCCG----------------------------------------------------AGAATAGCCG`	`52 bp deleted → frameshift`
28.5	`CCGGCCTTGCA-----------------------------------------------TGGTGAGCTGCGAGAATAGCCG`	`47 bp deleted → frameshift`
27.3	`CCGGCCTTGCACATG------------------------------------------------AGCTGCGAGAATAGCCG`	`48 bp deleted → no frameshift`
26.0	`CCGGCCTTGC-----------------------------------------------------------GAGAATAGCCG`	`59 bp deleted → frameshift`
25.6	`CC-------------------------------------------------------ATGGTGAGCTGCGAGAATAGCCG`	`55 bp deleted → frameshift`
24.4	`CCGGCCTTGC--------------------------------------------------------TGCGAGAATAGCCG`	`56 bp deleted → frameshift`
23.4	`CCGGCCTTG---------------------------------------------------GTGAGCTGCGAGAATAGCCG`	`51 bp deleted → no frameshift`
23.2	`CCGG---------------------------------------------------------TGAGCTGCGAGAATAGCCG`	`57 bp deleted → no frameshift`
21.3	`CCGGCCTTGCACATGCCGGA-----------------------------------------------------ATAGCCG`	`53 bp deleted → frameshift`
20.1	`CCGGCCTTG------------------------------------------------------AGCTGCGAGAATAGCCG`	`54 bp deleted → no frameshift`
18.8	`CCGGC-------------------------------------------------------------TGCGAGAATAGCCG`	`61 bp deleted → frameshift`
16.4	`CCGGC----------------------------------------------------------------GAGAATAGCCG`	`64 bp deleted → frameshift`
15.6	`CCGGCC-------------------------------------------------------------------------G`	`73 bp deleted → frameshift`
15.0	`CCGGCCT------------------------------------------------------------GCGAGAATAGCCG`	`60 bp deleted → no frameshift`
14.0	`CCG-------------------------------------------------------------------AGAATAGCCG`	`67 bp deleted → frameshift`
13.2	`CCGGCCTTGC--------------------------------------------------------------------CG`	`68 bp deleted → frameshift`
12.6	`CCG-----------------------------------------------------------------------------`	`77 bp deleted → frameshift`
9.4	`CCGGCCTTGCACAT-------------------------------------------------------------AGCCG`	`61 bp deleted → frameshift`

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