CRISPOR

Micro-homology scoring of potential deletions

Guide: GCGCCGGAAGTGGCCAGGGC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGCGGGGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	(Wild-type sequence)
592.8	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`6 bp deleted → no frameshift`
491.4	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGG----GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`4 bp deleted → frameshift`
467.4	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGG-----GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`5 bp deleted → frameshift`
344.4	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGG---GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`3 bp deleted → no frameshift`
329.4	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC------------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`12 bp deleted → no frameshift`
283.2	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC---------------TCACAGCGCGCCCGGCTATTCTCGC`	`15 bp deleted → no frameshift`
282.0	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGG-------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`7 bp deleted → frameshift`
255.2	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGG---------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`9 bp deleted → no frameshift`
242.8	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGG----------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`10 bp deleted → frameshift`
230.8	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGG-----------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`11 bp deleted → frameshift`
218.4	`TGGCGGCCTAAGGACTCGGCG--------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`26 bp deleted → frameshift`
210.0	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC---------------------TCACAGCGCGCCCGGCTATTCTCGC`	`21 bp deleted → no frameshift`
191.4	`TGGCGGCCTAAGGACTCGGC-----------------------------------TCACAGCGCGCCCGGCTATTCTCGC`	`35 bp deleted → frameshift`
188.8	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC---------------TCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`15 bp deleted → no frameshift`
188.8	`TGGCGGCCTAAGGACTCGGCGCGCCGG---------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`15 bp deleted → no frameshift`
180.6	`TGGCGGCCTAAGGACTCGGCGCG------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`24 bp deleted → no frameshift`
179.6	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGG----------------CTCACAGCGCGCCCGGCTATTCTCGC`	`16 bp deleted → frameshift`
179.6	`TGGCGGCCTAAGGACTCGGCGCGCCGG----------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`16 bp deleted → frameshift`
170.8	`TGGCGGCCTAAGGACTCGGCGCGCCGG-----------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`17 bp deleted → frameshift`
170.8	`TGGCGGCCTAAGGACTCGGCGCGCC------------------------------------------CGGCTATTCTCGC`	`42 bp deleted → no frameshift`
162.8	`TGGCGGCCTAAGGACTCGGCGCGCCGG------------------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`18 bp deleted → no frameshift`
155.4	`TGGCGGCCTAAGGACTCGGCGCGCCGG---------------------------CTCACAGCGCGCCCGGCTATTCTCGC`	`27 bp deleted → no frameshift`
150.5	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAG------------------------CGCGCCCGGCTATTCTCGC`	`24 bp deleted → no frameshift`
140.0	`TGGCGGCCTAAGGACTCGGCGCGCCG---------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`21 bp deleted → no frameshift`
133.2	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC----------------------GCGCCCGGCTATTCTCGC`	`22 bp deleted → frameshift`
133.2	`TGGCGGCCTAAGGACTCGGCGCGC----------------------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`22 bp deleted → frameshift`
127.2	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC-------------------------------TATTCTCGC`	`31 bp deleted → frameshift`
126.8	`TGGCGGCCTAAGGACTCGG-----------------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`23 bp deleted → frameshift`
121.2	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC--------------------------------CGGCTATTCTCGC`	`32 bp deleted → frameshift`
120.4	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC------------------------GCCCGGCTATTCTCGC`	`24 bp deleted → no frameshift`
120.4	`TGGCGGCCTAAGGACTCGG------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`24 bp deleted → no frameshift`
114.8	`TGGCGGCCTAAGGACTCGGCGCGCC-------------------------TCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`25 bp deleted → frameshift`
114.8	`TGGCGGCCTAAGGACTCGG-------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`25 bp deleted → frameshift`
110.4	`TGGCGGCCTAAGGACTCGGCGCGCCGGA--------------------CCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`20 bp deleted → frameshift`
109.2	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC--------------------------CCGGCTATTCTCGC`	`26 bp deleted → frameshift`
108.0	`TGGCGGCCTAAGGACTCGGCGCGC----------------------------------------GCCCGGCTATTCTCGC`	`40 bp deleted → frameshift`
99.9	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCA----------------------CAGCGCGCCCGGCTATTCTCGC`	`22 bp deleted → frameshift`
98.8	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC----------------------------GCGCCCGGCTATTCTCGC`	`28 bp deleted → frameshift`
97.6	`TGGCG------------------------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`42 bp deleted → no frameshift`
94.2	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC-------------------------------------TATTCTCGC`	`37 bp deleted → frameshift`
94.0	`TGGCGGCCTAAGGACTCG-----------------------------ACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`29 bp deleted → frameshift`
94.0	`TGGCGGCCTAAGG-----------------------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`29 bp deleted → frameshift`
92.8	`TGGCGGCCTAAGGACTCGGCGCGCCGG-------------------------------------------CTATTCTCGC`	`43 bp deleted → frameshift`
91.5	`TGGCGGCCTAAGGAC----------------------------------CTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`34 bp deleted → frameshift`
89.2	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC------------------------------GCCCGGCTATTCTCGC`	`30 bp deleted → no frameshift`
89.2	`TGGCGGCCTAAGGACTCGGCGCG------------------------------GCTCACAGCGCGCCCGGCTATTCTCGC`	`30 bp deleted → no frameshift`
89.2	`TGGCGGCCTAAGG------------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`30 bp deleted → no frameshift`
88.8	`TGGCGGCCTAAGGACTCGGCGC--------------------------------------------CCGGCTATTCTCGC`	`44 bp deleted → frameshift`
85.2	`TGGCGGC---------------------------------------GACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`39 bp deleted → no frameshift`
84.8	`TGGCGGCCTAAGGACTCGGCGCGC-------------------------------TCACAGCGCGCCCGGCTATTCTCGC`	`31 bp deleted → frameshift`
84.8	`TGGCGGCCTAAGG-------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`31 bp deleted → frameshift`
80.8	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGG--------------------------------CTATTCTCGC`	`32 bp deleted → frameshift`
80.8	`TGGCGGCCTAAGGACTCGGCG--------------------------------GCTCACAGCGCGCCCGGCTATTCTCGC`	`32 bp deleted → frameshift`
80.8	`TGGCGGCCTAAGG--------------------------------CGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`32 bp deleted → frameshift`
76.8	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCC---------------------------------GGCTATTCTCGC`	`33 bp deleted → no frameshift`
76.8	`TGGCGGCCTAAGGACTCGGCGC---------------------------------TCACAGCGCGCCCGGCTATTCTCGC`	`33 bp deleted → no frameshift`
72.8	`TGGCGGC------------------------------------------------TCACAGCGCGCCCGGCTATTCTCGC`	`48 bp deleted → no frameshift`
67.5	`TGGCGGCCTAAGGACTC----------------------------------------ACAGCGCGCCCGGCTATTCTCGC`	`40 bp deleted → frameshift`
66.0	`TGGCGG------------------------------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`36 bp deleted → no frameshift`
63.6	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAG-------------------------------CGCGCCCGGCTATTCTCGC`	`31 bp deleted → frameshift`
62.8	`TGGCGGCCTAAGGACTCGGCGCGCCG-------------------------------------CGCCCGGCTATTCTCGC`	`37 bp deleted → frameshift`
62.8	`TGGCGG-------------------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`37 bp deleted → frameshift`
62.4	`TGGCGGCCTAAGGACTCGGC---------------------------------------------------TATTCTCGC`	`51 bp deleted → no frameshift`
61.0	`TGGCGGCCT------------------------------------------CGGCTCACAGCGCGCCCGGCTATTCTCGC`	`42 bp deleted → no frameshift`
60.0	`TGGCGGCCTAAGGACTCGGCGCGC--------------------------------------GCGCCCGGCTATTCTCGC`	`38 bp deleted → frameshift`
60.0	`TGGCGG--------------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`38 bp deleted → frameshift`
56.8	`TGGCGGCCTAAGGACTCGGCGCGCCG---------------------------------------CCCGGCTATTCTCGC`	`39 bp deleted → no frameshift`
56.8	`TGG---------------------------------------GGGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`39 bp deleted → no frameshift`
54.0	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCAGGGC----------------------------------------`	`40 bp deleted → frameshift`
54.0	`TGG----------------------------------------GGCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`40 bp deleted → frameshift`
51.6	`TGGCGGCCTAAGG-----------------------------------------CTCACAGCGCGCCCGGCTATTCTCGC`	`41 bp deleted → frameshift`
51.6	`TGG-----------------------------------------GCGACCTCGGCTCACAGCGCGCCCGGCTATTCTCGC`	`41 bp deleted → frameshift`
46.8	`TGGC---------------------------------------------------TCACAGCGCGCCCGGCTATTCTCGC`	`51 bp deleted → no frameshift`
42.0	`TGGCGGCCTAAGGACTCG---------------------------------------------CGCCCGGCTATTCTCGC`	`45 bp deleted → no frameshift`
40.0	`TGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGC----------------------------------------------`	`46 bp deleted → frameshift`
40.0	`TGGCGGCCTAAGGACTCGGCGCG----------------------------------------------GCTATTCTCGC`	`46 bp deleted → frameshift`
40.0	`TGGCGGCCTAAGGACTCGGC----------------------------------------------CCGGCTATTCTCGC`	`46 bp deleted → frameshift`
38.0	`TGGCGGCCTAAGGACTCGGCGCGC-----------------------------------------------TATTCTCGC`	`47 bp deleted → frameshift`
38.0	`TGGCGGCCTAAGGACTCG-----------------------------------------------CCCGGCTATTCTCGC`	`47 bp deleted → frameshift`
36.6	`TGGCGGCCTAAGGACTCGGCGCGC--------------------------------------------------------`	`56 bp deleted → frameshift`
36.4	`TGGCGGCCTAAGGACTCGGCG------------------------------------------------GCTATTCTCGC`	`48 bp deleted → no frameshift`
34.4	`TGGCGGCCTAAGGACTCGGCGC-------------------------------------------------TATTCTCGC`	`49 bp deleted → frameshift`
33.3	`TGGCGGCCTAAGGAC--------------------------------------------AGCGCGCCCGGCTATTCTCGC`	`44 bp deleted → frameshift`
33.0	`TGGCGGCCTAAGGACTCGGCGC----------------------------------------------------------`	`58 bp deleted → frameshift`
33.0	`TGGCG----------------------------------------------------------CGCCCGGCTATTCTCGC`	`58 bp deleted → frameshift`
32.9	`TGGCGGCCTAAGGACTCG-------------------------------------------------------------C`	`61 bp deleted → frameshift`
32.8	`TGGCGGC----------------------------------------------------------------TATTCTCGC`	`64 bp deleted → frameshift`
31.2	`TGGCGGCC-----------------------------------------------------------CGGCTATTCTCGC`	`59 bp deleted → frameshift`
30.0	`TGGCG------------------------------------------------------------CCCGGCTATTCTCGC`	`60 bp deleted → no frameshift`
28.5	`TGGCGGCCT-----------------------------------------------CACAGCGCGCCCGGCTATTCTCGC`	`47 bp deleted → frameshift`
28.4	`TGGCGGCCTAAGGACTCGGCGCGCCG-----------------------------------------------------C`	`53 bp deleted → frameshift`
25.8	`TGGCGGCCTAAG-------------------------------------------------CGCGCCCGGCTATTCTCGC`	`49 bp deleted → frameshift`
25.6	`TGGCGGC-------------------------------------------------------GCGCCCGGCTATTCTCGC`	`55 bp deleted → frameshift`
23.2	`TGGCGGCCTAAGG---------------------------------------------------------CTATTCTCGC`	`57 bp deleted → no frameshift`
23.2	`TGGCGGC---------------------------------------------------------GCCCGGCTATTCTCGC`	`57 bp deleted → no frameshift`
21.0	`TGGC-------------------------------------------------------------------TATTCTCGC`	`67 bp deleted → frameshift`
20.0	`TGGCGGCCTAAGGACTCGGC------------------------------------------------------------`	`60 bp deleted → no frameshift`
20.0	`TGGCGGCC------------------------------------------------------------GGCTATTCTCGC`	`60 bp deleted → no frameshift`
18.3	`TGGCGGCCTAAGGACT--------------------------------------------------------ATTCTCGC`	`56 bp deleted → frameshift`
18.0	`TGGC--------------------------------------------------------------CCGGCTATTCTCGC`	`62 bp deleted → frameshift`
17.2	`TGGCGGCCTA---------------------------------------------------------------TTCTCGC`	`63 bp deleted → no frameshift`
15.6	`TGGCGGCCTAAGGACTC-----------------------------------------------------------TCGC`	`59 bp deleted → frameshift`
10.4	`TGGCGGC-------------------------------------------------------------------------`	`73 bp deleted → frameshift`
10.0	`TGGCG--------------------------------------------------------------------------C`	`74 bp deleted → frameshift`
9.9	`TGGCGGCCT--------------------------------------------------------------------CGC`	`68 bp deleted → frameshift`
8.8	`TGGC----------------------------------------------------------------------------`	`76 bp deleted → frameshift`

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