CRISPOR

Micro-homology scoring of potential deletions

Guide: TCCCCAATCTGGGCGCGCGC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	(Wild-type sequence)
607.0	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGC----------CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`10 bp deleted → frameshift`
592.8	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGCGC------CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`6 bp deleted → no frameshift`
536.0	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGC--------CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`8 bp deleted → frameshift`
344.4	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGCG---GCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`3 bp deleted → no frameshift`
327.6	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGCGC----GCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`4 bp deleted → frameshift`
311.6	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCG-----GCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`5 bp deleted → frameshift`
294.4	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCG--------------------GCCTAAGGACTCGGCGCGCCGGAAG`	`20 bp deleted → frameshift`
282.0	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCG-------GCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`7 bp deleted → frameshift`
269.4	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGCG----------------GCCTAAGGACTCGGCGCGCCGGAAG`	`16 bp deleted → frameshift`
245.0	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGG---------------------CGGCCTAAGGACTCGGCGCGCCGGAAG`	`21 bp deleted → no frameshift`
244.2	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCG------------------GCCTAAGGACTCGGCGCGCCGGAAG`	`18 bp deleted → no frameshift`
240.8	`GGCCGGCCCGGCTTCCTTTGTCCCC------------------------CTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`24 bp deleted → no frameshift`
231.0	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGC------------------------------------CGGAAG`	`36 bp deleted → no frameshift`
230.8	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGG-----------CGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`11 bp deleted → frameshift`
229.6	`GGCCGGCCCGGCTTCCTTTGTCCCC-------------------------TGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`25 bp deleted → frameshift`
219.6	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGCGC----------------------------------CGGAAG`	`34 bp deleted → frameshift`
219.6	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGC------------CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`12 bp deleted → no frameshift`
198.8	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGCGC--------------GGCCTAAGGACTCGGCGCGCCGGAAG`	`14 bp deleted → frameshift`
190.2	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGC-----------------------CTAAGGACTCGGCGCGCCGGAAG`	`23 bp deleted → frameshift`
190.2	`GGCCGGCCCGGCTTCCTTTGTCCCC-----------------------CCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`23 bp deleted → frameshift`
170.8	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGCGC-----------------CTAAGGACTCGGCGCGCCGGAAG`	`17 bp deleted → frameshift`
163.8	`GGCCGGCCCGGCTTCCTTTGTCCC--------------------------TGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`26 bp deleted → frameshift`
161.6	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGCGC--------------------------------GCCGGAAG`	`32 bp deleted → frameshift`
161.6	`GGCCGGCCCGGC--------------------------------GCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`32 bp deleted → frameshift`
154.8	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGC-------------------CTAAGGACTCGGCGCGCCGGAAG`	`19 bp deleted → frameshift`
140.0	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGC---------------------CTAAGGACTCGGCGCGCCGGAAG`	`21 bp deleted → no frameshift`
133.2	`GGCCGGCCCGGCTTCCTTTGTCCCC----------------------CCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`22 bp deleted → frameshift`
125.6	`GGCCGGC-------------------------------------GCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`37 bp deleted → frameshift`
120.4	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGG------------------------CCTAAGGACTCGGCGCGCCGGAAG`	`24 bp deleted → no frameshift`
120.0	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGC--------------------------------------CGGAAG`	`38 bp deleted → frameshift`
113.6	`GGCCGGCCC---------------------------------------CCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`39 bp deleted → no frameshift`
103.6	`GGCCGGCCCGGCTTCCTTTGTCC---------------------------TGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`27 bp deleted → no frameshift`
94.0	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGCG-----------------------------GCGCGCCGGAAG`	`29 bp deleted → frameshift`
91.5	`GGCCGGCCCGGCTTCCT----------------------------------GGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`34 bp deleted → frameshift`
89.2	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGCGC------------------------------GCGCCGGAAG`	`30 bp deleted → no frameshift`
89.2	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGG------------------------------ACTCGGCGCGCCGGAAG`	`30 bp deleted → no frameshift`
84.8	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCG-------------------------------GCGCGCCGGAAG`	`31 bp deleted → frameshift`
84.8	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGG-------------------------------ACTCGGCGCGCCGGAAG`	`31 bp deleted → frameshift`
84.8	`GGCCGGCCCGGCTTCC-------------------------------CCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`31 bp deleted → frameshift`
84.0	`GGCCGGCCCGGC---------------------------------------------CTAAGGACTCGGCGCGCCGGAAG`	`45 bp deleted → no frameshift`
82.0	`GGCCGGCC--------------------------------------------------TAAGGACTCGGCGCGCCGGAAG`	`50 bp deleted → frameshift`
81.0	`GGCCGGCCC----------------------------------------CTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`40 bp deleted → frameshift`
80.8	`GGCCGGCCCGGCTTCC--------------------------------CCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`32 bp deleted → frameshift`
77.4	`GGCCGGCCC-----------------------------------------TGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`41 bp deleted → frameshift`
77.4	`GGC-----------------------------------------GCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`41 bp deleted → frameshift`
76.8	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCG---------------------------------GCGCGCCGGAAG`	`33 bp deleted → no frameshift`
76.8	`GGCCGGCCCGGCTTCCTTTGTCCCC---------------------------------TAAGGACTCGGCGCGCCGGAAG`	`33 bp deleted → no frameshift`
76.8	`GGCCGGCCCGGCTTCC---------------------------------CTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`33 bp deleted → no frameshift`
73.2	`GGCCGGCCCGGCTTCCTTTGTCCC----------------------------------TAAGGACTCGGCGCGCCGGAAG`	`34 bp deleted → frameshift`
73.2	`GGCCGGCCCGGC----------------------------------CCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`34 bp deleted → frameshift`
73.2	`GGCCGGCCCGGC------------------------------------------GGCCTAAGGACTCGGCGCGCCGGAAG`	`42 bp deleted → no frameshift`
70.5	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCT-----------------------------AAGGACTCGGCGCGCCGGAAG`	`29 bp deleted → frameshift`
69.6	`GGCC-------------------------------------------CCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`43 bp deleted → frameshift`
69.6	`GGCCGGCCCGGCTTCCTTTGTCC-----------------------------------TAAGGACTCGGCGCGCCGGAAG`	`35 bp deleted → frameshift`
69.6	`GGCCGGCCCG-----------------------------------CCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`35 bp deleted → frameshift`
62.8	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCGCG-------------------------------------GAAG`	`37 bp deleted → frameshift`
62.8	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGG-------------------------------------CGCGCCGGAAG`	`37 bp deleted → frameshift`
61.0	`GGCCGGCCCGGCTTCCT------------------------------------------AAGGACTCGGCGCGCCGGAAG`	`42 bp deleted → no frameshift`
60.6	`GGCCGGCCCGGCTTCCTTTG--------------------------------GCGGCCTAAGGACTCGGCGCGCCGGAAG`	`32 bp deleted → frameshift`
60.0	`GGCCGGCCC--------------------------------------CCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`38 bp deleted → frameshift`
57.0	`GGCCGGC-----------------------------------------------GGCCTAAGGACTCGGCGCGCCGGAAG`	`47 bp deleted → frameshift`
56.8	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCGCG---------------------------------------GAAG`	`39 bp deleted → no frameshift`
54.0	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGC----------------------------------------CGGAAG`	`40 bp deleted → frameshift`
54.0	`GGCCG----------------------------------------CCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`40 bp deleted → frameshift`
53.6	`GGCC------------------------------------------------------TAAGGACTCGGCGCGCCGGAAG`	`54 bp deleted → no frameshift`
51.6	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGGCG-----------------------------------------GAAG`	`41 bp deleted → frameshift`
49.5	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCT------------------------------------CGGCGCGCCGGAAG`	`36 bp deleted → no frameshift`
48.8	`GGCCGGCC------------------------------------------TGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`42 bp deleted → no frameshift`
46.8	`GGC---------------------------------------------------GGCCTAAGGACTCGGCGCGCCGGAAG`	`51 bp deleted → no frameshift`
45.0	`GGCCGGCCCGGCTTCCTTTGTCCCCAATC--------------------------------------GGCGCGCCGGAAG`	`38 bp deleted → frameshift`
45.0	`GGCCGGCCCGGCT--------------------------------------GGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`38 bp deleted → frameshift`
44.4	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGGG--------------------------------------------AAG`	`44 bp deleted → frameshift`
44.4	`GGCC--------------------------------------------CCTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`44 bp deleted → frameshift`
44.0	`GGCCGGCCCGGC----------------------------------------------------------GCGCCGGAAG`	`58 bp deleted → frameshift`
42.0	`GGCCGGCCCGGCTTCCTTTGTCCCCAATCTGG---------------------------------------------AAG`	`45 bp deleted → no frameshift`
42.0	`GGCC---------------------------------------------CTGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`45 bp deleted → no frameshift`
40.0	`GGCC----------------------------------------------TGGCGGCCTAAGGACTCGGCGCGCCGGAAG`	`46 bp deleted → frameshift`
36.6	`GGCCGGCCCGGCTTCCTTTGTCCCCAA----------------------------------GGACTCGGCGCGCCGGAAG`	`34 bp deleted → frameshift`
34.4	`GGCCGGCCC-------------------------------------------------TAAGGACTCGGCGCGCCGGAAG`	`49 bp deleted → frameshift`
34.4	`GGCCGGC---------------------------------------------------------------GCGCCGGAAG`	`63 bp deleted → no frameshift`
32.8	`GGCCGGCCCGGCTTCCTTTGTCCCC--------------------------------------------------GGAAG`	`50 bp deleted → frameshift`
31.5	`GGCCGGCCCGGCTTCCTTTGTC---------------------------------------------GGCGCGCCGGAAG`	`45 bp deleted → no frameshift`
31.2	`GGCCGGCCCGGCTTCCTTTGTCCC---------------------------------------------------GGAAG`	`51 bp deleted → no frameshift`
30.0	`GGCCGGCCCGGCT----------------------------------------------AAGGACTCGGCGCGCCGGAAG`	`46 bp deleted → frameshift`
29.6	`GGCCGGCCCGGCTTCCTTTGTCC----------------------------------------------------GGAAG`	`52 bp deleted → frameshift`
29.6	`GGCCGGCCCGG----------------------------------------------------ACTCGGCGCGCCGGAAG`	`52 bp deleted → frameshift`
29.6	`GGCCGGCCCGG------------------------------------------------------------------AAG`	`66 bp deleted → no frameshift`
29.0	`GGCCGG-----------------------------------------------------------------------AAG`	`71 bp deleted → frameshift`
25.8	`GGCCGGCCCGGCTTCCT-------------------------------------------------CGGCGCGCCGGAAG`	`49 bp deleted → frameshift`
23.2	`GGCCGG---------------------------------------------------------ACTCGGCGCGCCGGAAG`	`57 bp deleted → no frameshift`
22.2	`GGCCGGCCCGGCTTC----------------------------------------------------GGCGCGCCGGAAG`	`52 bp deleted → frameshift`
21.3	`GGCCGGCCCGGCT-----------------------------------------------------CGGCGCGCCGGAAG`	`53 bp deleted → frameshift`
21.0	`GGCCGGCC-------------------------------------------------------------------GGAAG`	`67 bp deleted → frameshift`
21.0	`GGC-------------------------------------------------------------------GCGCCGGAAG`	`67 bp deleted → frameshift`
20.8	`GGCCGGCCCGGCTTCC-----------------------------------------------------------GGAAG`	`59 bp deleted → frameshift`
20.0	`GGCCGGCCCGGC------------------------------------------------------------GCCGGAAG`	`60 bp deleted → no frameshift`
18.8	`GGCCGGCCCG-------------------------------------------------------------CGCCGGAAG`	`61 bp deleted → frameshift`
18.8	`GG-------------------------------------------------------------ACTCGGCGCGCCGGAAG`	`61 bp deleted → frameshift`
18.0	`GGCCGGCCCGGC--------------------------------------------------------------CGGAAG`	`62 bp deleted → frameshift`
17.2	`GGCCGGCCCG---------------------------------------------------------------CCGGAAG`	`63 bp deleted → no frameshift`
15.6	`GGCCGGC-----------------------------------------------------------------GCCGGAAG`	`65 bp deleted → frameshift`
14.8	`GGCCGGCCCGGCTTCCTTTGTCCCCAA----------------------------------------------------G`	`52 bp deleted → frameshift`
14.8	`GGCCG------------------------------------------------------------------CGCCGGAAG`	`66 bp deleted → no frameshift`
13.2	`GGCCG--------------------------------------------------------------------CCGGAAG`	`68 bp deleted → frameshift`
12.8	`GGC---------------------------------------------------------------------GCCGGAAG`	`69 bp deleted → no frameshift`
9.6	`GG---------------------------------------------------------------------------AAG`	`75 bp deleted → no frameshift`

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