CRISPOR

Micro-homology scoring of potential deletions

Guide: CTTAGGCCGCCAGGGGGCGC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	(Wild-type sequence)
741.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGC------GCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`6 bp deleted → no frameshift`
536.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGC--------GCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`8 bp deleted → frameshift`
485.6	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGC----------CCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`10 bp deleted → frameshift`
344.4	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCG---GCGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`3 bp deleted → no frameshift`
327.6	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGC----GCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`4 bp deleted → frameshift`
294.4	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCC--------------------CAGATTGGGGACAAAGGAAGCCGGGCCGG`	`20 bp deleted → frameshift`
292.8	`CACTTCCGGCGCGC----------------------------------GCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`34 bp deleted → frameshift`
282.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG-------CGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`7 bp deleted → frameshift`
269.4	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGC----------------GCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`16 bp deleted → frameshift`
268.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGG--------CGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`8 bp deleted → frameshift`
256.2	`CACTTCCGGCGCGCCGAGTCCTTAGGC-----------------GCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`17 bp deleted → frameshift`
255.2	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGG---------CGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`9 bp deleted → no frameshift`
245.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG---------------------ATTGGGGACAAAGGAAGCCGGGCCGG`	`21 bp deleted → no frameshift`
244.2	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGC------------------GCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`18 bp deleted → no frameshift`
240.8	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGG------------------------ACAAAGGAAGCCGGGCCGG`	`24 bp deleted → no frameshift`
231.0	`CACTTCCGGCGCGCC------------------------------------CAGATTGGGGACAAAGGAAGCCGGGCCGG`	`36 bp deleted → no frameshift`
229.6	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG-------------------------ACAAAGGAAGCCGGGCCGG`	`25 bp deleted → frameshift`
219.6	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGC------------CCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`12 bp deleted → no frameshift`
208.8	`CACTTCCGGCGCGCCGAGTCCTTAGGCCG-------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`13 bp deleted → frameshift`
198.8	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGC--------------GCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`14 bp deleted → frameshift`
190.2	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGG-----------------------GACAAAGGAAGCCGGGCCGG`	`23 bp deleted → frameshift`
190.2	`CACTTCCGGCGCGCCGAGTCCTTAGGCC-----------------------CAGATTGGGGACAAAGGAAGCCGGGCCGG`	`23 bp deleted → frameshift`
163.8	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGG--------------------------ACAAAGGAAGCCGGGCCGG`	`26 bp deleted → frameshift`
161.6	`CACTTCCGGCGCGC--------------------------------GCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`32 bp deleted → frameshift`
154.8	`CACTTCCGGCGCGCCGAGTCCTTAGGC-------------------GCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`19 bp deleted → frameshift`
140.0	`CACTTCCGGCGCGCCGAGTCCTTAGGC---------------------GCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`21 bp deleted → no frameshift`
133.2	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGG----------------------GGACAAAGGAAGCCGGGCCGG`	`22 bp deleted → frameshift`
120.4	`CACTTCCGGCGCGCCGAGTCCTTAGGCC------------------------AGATTGGGGACAAAGGAAGCCGGGCCGG`	`24 bp deleted → no frameshift`
120.0	`CACTTCCGGCGC--------------------------------------CCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`38 bp deleted → frameshift`
113.6	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGC---------------------------------------CGG`	`39 bp deleted → no frameshift`
109.2	`CACTTCCGGCGCGCCG--------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`26 bp deleted → frameshift`
103.6	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGG---------------------------ACAAAGGAAGCCGGGCCGG`	`27 bp deleted → no frameshift`
94.0	`CACTTCCGGCGCGCCG-----------------------------CGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`29 bp deleted → frameshift`
94.0	`CACTTCCGGCGCG-----------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`29 bp deleted → frameshift`
91.5	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGG----------------------------------AAGCCGGGCCGG`	`34 bp deleted → frameshift`
89.2	`CACTTCCGGCGCGCCGAGTCC------------------------------CAGATTGGGGACAAAGGAAGCCGGGCCGG`	`30 bp deleted → no frameshift`
89.2	`CACTTCCGGCGCGC------------------------------GCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`30 bp deleted → no frameshift`
84.8	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGG-------------------------------AAGCCGGGCCGG`	`31 bp deleted → frameshift`
84.8	`CACTTCCGGCGCGCCGAGTCC-------------------------------AGATTGGGGACAAAGGAAGCCGGGCCGG`	`31 bp deleted → frameshift`
84.8	`CACTTCCGGCGCGCCG-------------------------------CGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`31 bp deleted → frameshift`
84.8	`CACTTCCGGCG-------------------------------GCGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`31 bp deleted → frameshift`
84.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCG---------------------------------------------GGCCGG`	`45 bp deleted → no frameshift`
82.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCG--------------------------------------------------G`	`50 bp deleted → frameshift`
81.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG----------------------------------------CCGG`	`40 bp deleted → frameshift`
80.8	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGC--------------------------------CGGGCCGG`	`32 bp deleted → frameshift`
80.8	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG--------------------------------AAGCCGGGCCGG`	`32 bp deleted → frameshift`
77.4	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGG-----------------------------------------CCGG`	`41 bp deleted → frameshift`
76.8	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGG---------------------------------AAGCCGGGCCGG`	`33 bp deleted → no frameshift`
76.8	`CACTTCCGGCGCGCCGAGTCCTTAGG---------------------------------GGACAAAGGAAGCCGGGCCGG`	`33 bp deleted → no frameshift`
76.8	`CACTTCCGGCGCGCCG---------------------------------CCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`33 bp deleted → no frameshift`
73.2	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGC----------------------------------CGGGCCGG`	`34 bp deleted → frameshift`
73.2	`CACTTCCGGCGCGCCGAGTCCTTAGG----------------------------------GACAAAGGAAGCCGGGCCGG`	`34 bp deleted → frameshift`
73.2	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCC------------------------------------------GGGCCGG`	`42 bp deleted → no frameshift`
70.5	`CACTTCCGGCGCGCCGAGTCCTTAG-----------------------------ATTGGGGACAAAGGAAGCCGGGCCGG`	`29 bp deleted → frameshift`
69.6	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCG-----------------------------------GGCCGG`	`35 bp deleted → frameshift`
69.6	`CACTTCCGGCGCGCCGAGTCCTTAGG-----------------------------------ACAAAGGAAGCCGGGCCGG`	`35 bp deleted → frameshift`
62.8	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCGC-------------------------------------CGG`	`37 bp deleted → frameshift`
62.8	`CACTTCCGGCGCGCC-------------------------------------AGATTGGGGACAAAGGAAGCCGGGCCGG`	`37 bp deleted → frameshift`
62.8	`CACTTCCG-------------------------------------CGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`37 bp deleted → frameshift`
61.0	`CACTTCCGGCGCGCCGAGTCCTTAGG------------------------------------------AAGCCGGGCCGG`	`42 bp deleted → no frameshift`
60.6	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCA--------------------------------AAGGAAGCCGGGCCGG`	`32 bp deleted → frameshift`
60.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGG--------------------------------------GCCGG`	`38 bp deleted → frameshift`
57.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCC-----------------------------------------------GG`	`47 bp deleted → frameshift`
56.8	`CACTTCCG---------------------------------------CGCCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`39 bp deleted → no frameshift`
54.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGGCG----------------------------------------G`	`40 bp deleted → frameshift`
54.0	`CACTTCCGGC----------------------------------------CCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`40 bp deleted → frameshift`
51.6	`CACTTCCG-----------------------------------------CCCAGATTGGGGACAAAGGAAGCCGGGCCGG`	`41 bp deleted → frameshift`
49.5	`CACTTCCGGCGCGCCGAG------------------------------------ATTGGGGACAAAGGAAGCCGGGCCGG`	`36 bp deleted → no frameshift`
48.8	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGG------------------------------------------CCGG`	`42 bp deleted → no frameshift`
46.4	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGGG-------------------------------------------`	`43 bp deleted → frameshift`
45.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG--------------------------------------CCGGGCCGG`	`38 bp deleted → frameshift`
45.0	`CACTTCCGGCGCGCCGA--------------------------------------TTGGGGACAAAGGAAGCCGGGCCGG`	`38 bp deleted → frameshift`
44.4	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGGG--------------------------------------------`	`44 bp deleted → frameshift`
44.4	`CACTTCC--------------------------------------------CAGATTGGGGACAAAGGAAGCCGGGCCGG`	`44 bp deleted → frameshift`
44.0	`CACTTCCGGCGCGCCG----------------------------------------------------------GGCCGG`	`58 bp deleted → frameshift`
42.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGGG---------------------------------------------`	`45 bp deleted → no frameshift`
42.0	`CACTTCC---------------------------------------------AGATTGGGGACAAAGGAAGCCGGGCCGG`	`45 bp deleted → no frameshift`
40.0	`CACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAGG----------------------------------------------`	`46 bp deleted → frameshift`
36.6	`CACTTCCGGCGCGCCGAGTCCTT----------------------------------GGGGACAAAGGAAGCCGGGCCGG`	`34 bp deleted → frameshift`
34.4	`CACTTCCGGCGCGCCGAGTCCTTAGG-------------------------------------------------GCCGG`	`49 bp deleted → frameshift`
34.4	`CACTTCCGGCGCGCCG---------------------------------------------------------------G`	`63 bp deleted → no frameshift`
32.8	`CACTTCCGG--------------------------------------------------GGACAAAGGAAGCCGGGCCGG`	`50 bp deleted → frameshift`
31.5	`CACTTCCGGCGCGCCGA---------------------------------------------CAAAGGAAGCCGGGCCGG`	`45 bp deleted → no frameshift`
31.2	`CACTTCCGG---------------------------------------------------GACAAAGGAAGCCGGGCCGG`	`51 bp deleted → no frameshift`
30.0	`CACTTCCGGCGCGCCGAGTCCTTAG----------------------------------------------CCGGGCCGG`	`46 bp deleted → frameshift`
29.6	`CACTTCCGGCGCGCCGAGTCC----------------------------------------------------GGGCCGG`	`52 bp deleted → frameshift`
29.6	`CACTTCCGG----------------------------------------------------ACAAAGGAAGCCGGGCCGG`	`52 bp deleted → frameshift`
29.6	`CACTTCCGG------------------------------------------------------------------GCCGG`	`66 bp deleted → no frameshift`
26.8	`CACTTCCGGCGCGCCGAGTCCTTAGG------------------------------------------------------`	`54 bp deleted → no frameshift`
25.8	`CACTTCCGGCGCGCCGAG-------------------------------------------------GAAGCCGGGCCGG`	`49 bp deleted → frameshift`
23.4	`CA---------------------------------------------------GATTGGGGACAAAGGAAGCCGGGCCGG`	`51 bp deleted → no frameshift`
23.2	`CACTTCCGGCGCGCCGAGTCC---------------------------------------------------------GG`	`57 bp deleted → no frameshift`
23.2	`CACTTCCGG-----------------------------------------------------------------------`	`71 bp deleted → frameshift`
22.2	`CACTTCCGGCGCGCCGA----------------------------------------------------AGCCGGGCCGG`	`52 bp deleted → frameshift`
21.3	`CACTTCCGGCGCGCCGAG-----------------------------------------------------CCGGGCCGG`	`53 bp deleted → frameshift`
21.0	`CACTTCCGGC-------------------------------------------------------------------CGG`	`67 bp deleted → frameshift`
20.8	`CACTTCCGG-----------------------------------------------------------AAGCCGGGCCGG`	`59 bp deleted → frameshift`
20.0	`CACTTCCGGCGC------------------------------------------------------------CGGGCCGG`	`60 bp deleted → no frameshift`
18.8	`CACTTCCGGCGCG-------------------------------------------------------------GGCCGG`	`61 bp deleted → frameshift`
18.0	`CACTTCCGGC--------------------------------------------------------------CGGGCCGG`	`62 bp deleted → frameshift`
17.2	`CACTTCCGGCG---------------------------------------------------------------GGCCGG`	`63 bp deleted → no frameshift`
15.6	`CACTTCCGGCGC-----------------------------------------------------------------CGG`	`65 bp deleted → frameshift`
15.0	`CAC------------------------------------------------------------AAAGGAAGCCGGGCCGG`	`60 bp deleted → no frameshift`
14.8	`CACTTCCGGCGCG------------------------------------------------------------------G`	`66 bp deleted → no frameshift`
14.8	`CACTT----------------------------------------------------GGGGACAAAGGAAGCCGGGCCGG`	`52 bp deleted → frameshift`
13.5	`CA--------------------------------------------------------------AAGGAAGCCGGGCCGG`	`62 bp deleted → frameshift`
13.2	`CACTTCCGGCG--------------------------------------------------------------------G`	`68 bp deleted → frameshift`

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