CRISPOR

Micro-homology scoring of potential deletions

Guide: CTCGTCGTCGACAACGGCTC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	(Wild-type sequence)
592.8	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGC------ATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`6 bp deleted → no frameshift`
315.0	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCT---------------------TCGCGGGCGACGATGCCCCC`	`21 bp deleted → no frameshift`
256.2	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGC-----------------CGGCTTCGCGGGCGACGATGCCCCC`	`17 bp deleted → frameshift`
219.6	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGC------------AAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`12 bp deleted → no frameshift`
219.6	`TGGATGATGATATCGCCGCGCTCGTCGTCG------------GCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`12 bp deleted → no frameshift`
188.8	`TGGATGATGATATCGCCGCGCTCGTCG---------------GCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`15 bp deleted → no frameshift`
164.7	`TGGATGATGATATCGCCGCGCTCGTCGTCGACA------------TGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`12 bp deleted → no frameshift`
162.8	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAA------------------GGCCGGCTTCGCGGGCGACGATGCCCCC`	`18 bp deleted → no frameshift`
162.8	`TGGATGATGATATCGCCGCGCTCG------------------GCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`18 bp deleted → no frameshift`
141.0	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACGG-----------------------------GCGACGATGCCCCC`	`29 bp deleted → frameshift`
133.8	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGC------------------------------GACGATGCCCCC`	`30 bp deleted → no frameshift`
133.2	`TGGATGATGATATCGCCGCG----------------------GCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`22 bp deleted → frameshift`
126.8	`TGGATGATGATATCGCCGCGC-----------------------ATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`23 bp deleted → frameshift`
120.4	`TGGATGATGATATCGCCG------------------------GCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`24 bp deleted → no frameshift`
114.8	`TGGATGATGATATCGCCGC-------------------------ATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`25 bp deleted → frameshift`
113.6	`TGGATGATGATATCGCCG---------------------------------------GCTTCGCGGGCGACGATGCCCCC`	`39 bp deleted → no frameshift`
110.4	`TGGATGATGATATCGCCGCGCTCGTCGT--------------------GCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`20 bp deleted → frameshift`
109.2	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGC--------------------------GGGCGACGATGCCCCC`	`26 bp deleted → frameshift`
103.6	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACG---------------------------CGGGCGACGATGCCCCC`	`27 bp deleted → no frameshift`
103.6	`TGGATGATGATATCGCCGCGCTCGTCGTCG---------------------------GCTTCGCGGGCGACGATGCCCCC`	`27 bp deleted → no frameshift`
103.6	`TGGATGATGATATCG---------------------------GCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`27 bp deleted → no frameshift`
99.9	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTC----------------------GCGGGCGACGATGCCCCC`	`22 bp deleted → frameshift`
99.4	`TGGATGATGATATCGCCGCGCTCGTCGTCGAC---------------------------------------GATGCCCCC`	`39 bp deleted → no frameshift`
98.8	`TGGATGATGATATCGC----------------------------ATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`28 bp deleted → frameshift`
96.0	`TGGATGATGATATCGCCGCGCTCGTCGTCG---------------------------------CGGGCGACGATGCCCCC`	`33 bp deleted → no frameshift`
95.1	`TGGATGATGATATCGCCGCGCTCGT-----------------------GCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`23 bp deleted → frameshift`
94.0	`TGGATGATGATATCGCCGCGC-----------------------------AAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`29 bp deleted → frameshift`
89.2	`TGGATGATGATATCGCCGCGCTCGTCG------------------------------GCTTCGCGGGCGACGATGCCCCC`	`30 bp deleted → no frameshift`
84.8	`TGGATGATGATATCGCCGC-------------------------------AAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`31 bp deleted → frameshift`
84.0	`TGGATGATGATATCGCCGCG---------------------------------------------GGCGACGATGCCCCC`	`45 bp deleted → no frameshift`
82.5	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACG------------------------------------ATGCCCCC`	`36 bp deleted → no frameshift`
82.5	`TGGATGATGATATCGCCGCGCTCGTCG------------------------------------CGGGCGACGATGCCCCC`	`36 bp deleted → no frameshift`
76.8	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACG---------------------------------ACGATGCCCCC`	`33 bp deleted → no frameshift`
76.8	`TGGATGATGATATCGCCGCGCTCG---------------------------------GCTTCGCGGGCGACGATGCCCCC`	`33 bp deleted → no frameshift`
75.0	`TGGATGATGATATCGCCGCGCT--------------------------------------TCGCGGGCGACGATGCCCCC`	`38 bp deleted → frameshift`
73.2	`TGGATGATGATATCGCCGCGC----------------------------------CGGCTTCGCGGGCGACGATGCCCCC`	`34 bp deleted → frameshift`
73.2	`TGGATGATGATATCGC----------------------------------AAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`34 bp deleted → frameshift`
71.0	`TGGATGATGATATCGCCGCGCTCG---------------------------------------CGGGCGACGATGCCCCC`	`39 bp deleted → no frameshift`
69.6	`TGGATGATGATATCGCCGCGC-------------------------------------------GGGCGACGATGCCCCC`	`43 bp deleted → frameshift`
69.6	`TGGATGATGATATCGCCGCGCTCGTCGTCG-----------------------------------GGCGACGATGCCCCC`	`35 bp deleted → frameshift`
66.0	`TGGATGATGATATCGCCGC------------------------------------CGGCTTCGCGGGCGACGATGCCCCC`	`36 bp deleted → no frameshift`
63.7	`TGGATGATGATATCGC------------------------------------------------GGGCGACGATGCCCCC`	`48 bp deleted → no frameshift`
62.8	`TGGATGATGATATCGCCGCG-------------------------------------GCTTCGCGGGCGACGATGCCCCC`	`37 bp deleted → frameshift`
61.0	`TGGATGATGATATCGCCGCGCTCGTCGTCGA------------------------------------------TGCCCCC`	`42 bp deleted → no frameshift`
60.0	`TGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGC--------------------------------------CCCC`	`38 bp deleted → frameshift`
60.0	`TGGATGATGATATCGCCGCGCTCGTCG--------------------------------------GGCGACGATGCCCCC`	`38 bp deleted → frameshift`
60.0	`TGGATGATG--------------------------------------TGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`38 bp deleted → frameshift`
54.0	`TGGATGATGATATCGCCGC----------------------------------------TTCGCGGGCGACGATGCCCCC`	`40 bp deleted → frameshift`
54.0	`TGG----------------------------------------CATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`40 bp deleted → frameshift`
51.6	`TGGATGATGATATCGCCGCGCTCG-----------------------------------------GGCGACGATGCCCCC`	`41 bp deleted → frameshift`
51.6	`TGGATG-----------------------------------------TGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`41 bp deleted → frameshift`
51.6	`TGGATGATGATATCGCCGCG-------------------------------------------------ACGATGCCCCC`	`49 bp deleted → frameshift`
48.8	`TGGATGATGATATCGCCGCGCTCGTCG------------------------------------------ACGATGCCCCC`	`42 bp deleted → no frameshift`
48.8	`TGGATGATGATATCG------------------------------------------GCTTCGCGGGCGACGATGCCCCC`	`42 bp deleted → no frameshift`
46.4	`TGGATGATGATATCGC-------------------------------------------TTCGCGGGCGACGATGCCCCC`	`43 bp deleted → frameshift`
42.0	`TGGATGATGATATCGCCGCGCTCGTCG---------------------------------------------ATGCCCCC`	`45 bp deleted → no frameshift`
42.0	`TGGATGATGATATCGCCGCGCTCG---------------------------------------------ACGATGCCCCC`	`45 bp deleted → no frameshift`
40.5	`TGGATGATG----------------------------------------CAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`40 bp deleted → frameshift`
38.4	`TGGATGATGATAT---------------------------------GTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`33 bp deleted → no frameshift`
38.0	`TGGATGATGATATCGCCGCGC-----------------------------------------------GACGATGCCCCC`	`47 bp deleted → frameshift`
38.0	`TGGATGATGATATCGCCG-----------------------------------------------GGCGACGATGCCCCC`	`47 bp deleted → frameshift`
36.4	`TGGATGATGATATCGCCGCGCTCG------------------------------------------------ATGCCCCC`	`48 bp deleted → no frameshift`
34.8	`TGGATG-------------------------------------------CAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`43 bp deleted → frameshift`
34.8	`TGGATGATGAT-----------------------------------GTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`35 bp deleted → frameshift`
32.8	`TGGATGATGATATCG--------------------------------------------------GGCGACGATGCCCCC`	`50 bp deleted → frameshift`
31.5	`TG---------------------------------------------TGCAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`45 bp deleted → no frameshift`
31.2	`TGGATGATGATATCGCCG---------------------------------------------------ACGATGCCCCC`	`51 bp deleted → no frameshift`
31.2	`TGG---------------------------------------------------CCGGCTTCGCGGGCGACGATGCCCCC`	`51 bp deleted → no frameshift`
30.0	`TGGATGATGATATCGCC------------------------------------------------------------CCC`	`60 bp deleted → no frameshift`
29.6	`TGGATGATGATATCGCCGCG----------------------------------------------------ATGCCCCC`	`52 bp deleted → frameshift`
29.6	`TGGATGATGATATCGC----------------------------------------------------GACGATGCCCCC`	`52 bp deleted → frameshift`
28.5	`TG-----------------------------------------------CAAGGCCGGCTTCGCGGGCGACGATGCCCCC`	`47 bp deleted → frameshift`
26.8	`TGGATGATGATATCGCCG------------------------------------------------------ATGCCCCC`	`54 bp deleted → no frameshift`
26.8	`TGGATGATGATATCG------------------------------------------------------ACGATGCCCCC`	`54 bp deleted → no frameshift`
25.6	`TGGATGATGATATCGCCGCGC-------------------------------------------------------CCCC`	`55 bp deleted → frameshift`
25.6	`TGG-------------------------------------------------------CTTCGCGGGCGACGATGCCCCC`	`55 bp deleted → frameshift`
23.2	`TGGATGATGATATCGCCGC---------------------------------------------------------CCCC`	`57 bp deleted → no frameshift`
23.2	`TGGATGATGATATCG---------------------------------------------------------ATGCCCCC`	`57 bp deleted → no frameshift`
22.2	`TGGATGATG------------------------------------------------------------------CCCCC`	`66 bp deleted → no frameshift`
19.2	`TGGATG---------------------------------------------------------------------CCCCC`	`69 bp deleted → no frameshift`
18.8	`TGGATGATGATATCGCC-------------------------------------------------------------CC`	`61 bp deleted → frameshift`
18.0	`TGGATGATGATATCGCC--------------------------------------------------------------C`	`62 bp deleted → frameshift`
17.2	`TGGATGATGATATCGCC---------------------------------------------------------------`	`63 bp deleted → no frameshift`
17.2	`TGGATGATGAT---------------------------------------------------------------GCCCCC`	`63 bp deleted → no frameshift`
17.2	`TGG---------------------------------------------------------------GCGACGATGCCCCC`	`63 bp deleted → no frameshift`
16.4	`TGG----------------------------------------------------------------CGACGATGCCCCC`	`64 bp deleted → frameshift`
15.0	`TGGATGATGA------------------------------------------------------------CGATGCCCCC`	`60 bp deleted → no frameshift`
12.9	`TGGATGA---------------------------------------------------------------CGATGCCCCC`	`63 bp deleted → no frameshift`
11.1	`TGGA------------------------------------------------------------------CGATGCCCCC`	`66 bp deleted → no frameshift`
9.4	`TGGATGATGATAT-------------------------------------------------------------GCCCCC`	`61 bp deleted → frameshift`
7.8	`TG-------------------------------------------------------------------------CCCCC`	`73 bp deleted → frameshift`

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