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Contents:

PCR primers for off-targets of GCGCCGGAAGTGGCCAGGGC GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

NamePrimer Sequence Tm CFD Score
ontarget_mm0__chr7 5.53 Mbp_chr7_5529702_F TCGTCGGCAGCGTCTGGTGAGCTGCGAGAATAGC 60.1 1.00
ontarget_mm0__chr7 5.53 Mbp_chr7_5529702_R GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT 60.6 1.00
mm4__chr20 59.45 Mbp_chr20_59452488_F TCGTCGGCAGCGTCGCGATGCTTCAGAGAGCACT 60.4 0.46
mm4__chr20 59.45 Mbp_chr20_59452488_R GTCTCGTGGGCTCGGTACTGTCCCCTCTCTCCCAC 59.6 0.46
mm3__chr8 63.14 Mbp_chr8_63144011_F Primer3: not found at this Tm N.d. 0.45
mm3__chr8 63.14 Mbp_chr8_63144011_R Primer3: not found at this Tm N.d. 0.45
mm4__chr9 135.33 Mbp_chr9_135331477_F TCGTCGGCAGCGTCCCCTGAGAGGAGAGAGCAGA 59.7 0.42
mm4__chr9 135.33 Mbp_chr9_135331477_R GTCTCGTGGGCTCGGCAGTGCAAGGCCATGAACTC 59.4 0.42
mm4__chr10 58.90 Mbp_chr10_58895678_F Primer3: not found at this Tm N.d. 0.40
mm4__chr10 58.90 Mbp_chr10_58895678_R Primer3: not found at this Tm N.d. 0.40
mm4__chr19 48.63 Mbp_chr19_48629095_F TCGTCGGCAGCGTCTCGTTCTGTGTCTGACCTGC 59.9 0.40
mm4__chr19 48.63 Mbp_chr19_48629095_R GTCTCGTGGGCTCGGCACCGTTGAGGGACAGGATG 60.3 0.40
mm2__chr11 120.34 Mbp_chr11_120336285_F TCGTCGGCAGCGTCCTTCCCGAAGTCAGAGGGGA 60.6 0.38
mm2__chr11 120.34 Mbp_chr11_120336285_R GTCTCGTGGGCTCGGCCGGTTCCGGTTCTGGTTT 60.2 0.38
mm3__chr20 50.04 Mbp_chr20_50040349_F TCGTCGGCAGCGTCGGGCCTGAGACTCCGTAAAC 60.1 0.37
mm3__chr20 50.04 Mbp_chr20_50040349_R GTCTCGTGGGCTCGGCCTTCCCAGGATCTCCCACA 60.6 0.37
mm4__chr14 75.47 Mbp_chr14_75470352_F TCGTCGGCAGCGTCAGTTACGGCCGTTGTGTGTA 59.6 0.35
mm4__chr14 75.47 Mbp_chr14_75470352_R GTCTCGTGGGCTCGGAAGGATGGAAATGCACCCCG 60.6 0.35
mm4__chr8 100.78 Mbp_chr8_100780081_F Primer3: not found at this Tm N.d. 0.33
mm4__chr8 100.78 Mbp_chr8_100780081_R Primer3: not found at this Tm N.d. 0.33
mm4__chr9 134.94 Mbp_chr9_134938233_F TCGTCGGCAGCGTCACACTCTCCTCCCGGTGTT 60.1 0.33
mm4__chr9 134.94 Mbp_chr9_134938233_R GTCTCGTGGGCTCGGCAGAGCAGGGAAGAGCCAAA 59.9 0.33
mm4__chr2 241.75 Mbp_chr2_241753813_F Primer3: not found at this Tm N.d. 0.33
mm4__chr2 241.75 Mbp_chr2_241753813_R Primer3: not found at this Tm N.d. 0.33
mm3__chr11 1.20 Mbp_chr11_1204175_F Primer3: not found at this Tm N.d. 0.32
mm3__chr11 1.20 Mbp_chr11_1204175_R Primer3: not found at this Tm N.d. 0.32
mm4__chr22 47.78 Mbp_chr22_47784141_F TCGTCGGCAGCGTCATGAACATGGCAGAGGCAGG 60.3 0.32
mm4__chr22 47.78 Mbp_chr22_47784141_R GTCTCGTGGGCTCGGGCCTCTGAAGCTGCTGAAAC 59.4 0.32
mm4__chr3 98.52 Mbp_chr3_98522620_F TCGTCGGCAGCGTCCCACCTCCTGGGCCTCAC 61.7 0.32
mm4__chr3 98.52 Mbp_chr3_98522620_R GTCTCGTGGGCTCGGGATTTGTCCCAGGGCCGAG 60.4 0.32
mm4__chr5 85.17 Mbp_chr5_85168895_F Primer3: not found at this Tm N.d. 0.32
mm4__chr5 85.17 Mbp_chr5_85168895_R Primer3: not found at this Tm N.d. 0.32
mm4__chr8 144.42 Mbp_chr8_144416549_F TCGTCGGCAGCGTCGGTACTGCCACTAGCCTCCT 60.6 0.31
mm4__chr8 144.42 Mbp_chr8_144416549_R GTCTCGTGGGCTCGGTGGAAAGGTAACAGCCCCAC 59.8 0.31
mm3__chr3 168.10 Mbp_chr3_168095660_F TCGTCGGCAGCGTCCCAGATGCCTCCTGCCTTTT 60.3 0.30
mm3__chr3 168.10 Mbp_chr3_168095660_R GTCTCGTGGGCTCGGCAACAAAGTCGTGCAGTGGG 59.9 0.30
mm4__chr5 2.27 Mbp_chr5_2271664_F TCGTCGGCAGCGTCGGAAGGTGGTGAGAGGAGGA 60.2 0.29
mm4__chr5 2.27 Mbp_chr5_2271664_R GTCTCGTGGGCTCGGCTCCCTCCCCTCACCACTC 60.6 0.29
mm4__chr14 104.28 Mbp_chr14_104278638_F TCGTCGGCAGCGTCCCTGGTGGCTTGGGTAATGT 59.9 0.29
mm4__chr14 104.28 Mbp_chr14_104278638_R GTCTCGTGGGCTCGGACCGGAGACTTGAGGGGAAT 60.2 0.29
mm4__chr6 170.46 Mbp_chr6_170462184_F TCGTCGGCAGCGTCCTCCAGAAAGTCCCAGGCTG 60.0 0.28
mm4__chr6 170.46 Mbp_chr6_170462184_R GTCTCGTGGGCTCGGTTTGTCTGCCTGGTCCTCTC 59.3 0.28
mm4__chr14 99.56 Mbp_chr14_99563437_F TCGTCGGCAGCGTCGGAGACCTGGGAAACTGGC 60.0 0.27
mm4__chr14 99.56 Mbp_chr14_99563437_R GTCTCGTGGGCTCGGATTCCCAGATGGCCCAGTTG 60.0 0.27
mm4__chr17 77.36 Mbp_chr17_77364600_F TCGTCGGCAGCGTCAGAGCGGAGAGGTTCCTCTT 59.9 0.27
mm4__chr17 77.36 Mbp_chr17_77364600_R GTCTCGTGGGCTCGGTAGAACTGTGGGCTGGCAAG 59.9 0.27
mm4__chr15 75.29 Mbp_chr15_75294385_F TCGTCGGCAGCGTCAGTGAGCCTGGACAACAACG 60.5 0.27
mm4__chr15 75.29 Mbp_chr15_75294385_R GTCTCGTGGGCTCGGATGACAGGAGACAGCTGCAC 60.0 0.27
mm4__chr7 149.28 Mbp_chr7_149282074_F TCGTCGGCAGCGTCCTTCTTCCAGCAGCGCAAG 59.4 0.27
mm4__chr7 149.28 Mbp_chr7_149282074_R GTCTCGTGGGCTCGGCTGGTGCCGGAAGAGGTC 59.7 0.27
mm3__chr7 150.38 Mbp_chr7_150379305_F TCGTCGGCAGCGTCCAATGGAGTGGCTGGGCG 61.1 0.26
mm3__chr7 150.38 Mbp_chr7_150379305_R GTCTCGTGGGCTCGGTTAACGGGAACTGCCACCC 59.9 0.26
mm4__chr17 3.60 Mbp_chr17_3600763_F TCGTCGGCAGCGTCCAGAGCCCTTGGTGCTAACA 59.9 0.26
mm4__chr17 3.60 Mbp_chr17_3600763_R GTCTCGTGGGCTCGGCGGCAAGAAGAGGAGAGCAG 60.4 0.26
mm4__chr5 179.82 Mbp_chr5_179821852_F TCGTCGGCAGCGTCGGTTAGGGAGGTGGTTGTGG 59.9 0.26
mm4__chr5 179.82 Mbp_chr5_179821852_R GTCTCGTGGGCTCGGTAGGAGAGGGCCTGGTATGC 60.4 0.26
mm3__chr15 27.59 Mbp_chr15_27591258_F TCGTCGGCAGCGTCAACAGCTACTGTGCACTGGG 60.2 0.25
mm3__chr15 27.59 Mbp_chr15_27591258_R GTCTCGTGGGCTCGGGACATGCTGGGGATTCACCA 60.0 0.25
mm4__chr16 66.51 Mbp_chr16_66509922_F Primer3: not found at this Tm N.d. 0.25
mm4__chr16 66.51 Mbp_chr16_66509922_R Primer3: not found at this Tm N.d. 0.25
mm4__chr10 126.80 Mbp_chr10_126797175_F TCGTCGGCAGCGTCTGAGCATCATGGAGAGCACC 59.8 0.25
mm4__chr10 126.80 Mbp_chr10_126797175_R GTCTCGTGGGCTCGGGCTGAGTCCACCTGTGTACC 60.0 0.25
mm3__chr20 37.41 Mbp_chr20_37406613_F TCGTCGGCAGCGTCGTGGATGTGGGTGGGAACAG 60.6 0.24
mm3__chr20 37.41 Mbp_chr20_37406613_R GTCTCGTGGGCTCGGCTTTGTCCCGGTTGCGCTC 61.9 0.24
mm4__chr15 53.40 Mbp_chr15_53397338_F Primer3: not found at this Tm N.d. 0.24
mm4__chr15 53.40 Mbp_chr15_53397338_R Primer3: not found at this Tm N.d. 0.24
mm4__chr15 93.41 Mbp_chr15_93410726_F Primer3: not found at this Tm N.d. 0.24
mm4__chr15 93.41 Mbp_chr15_93410726_R Primer3: not found at this Tm N.d. 0.24
mm4__chr17 43.90 Mbp_chr17_43900231_F TCGTCGGCAGCGTCGGCTTGGCATTGGTCAGAGA 60.3 0.23
mm4__chr17 43.90 Mbp_chr17_43900231_R GTCTCGTGGGCTCGGGGGAATCTGGCATACCCTGG 59.8 0.23
mm4__chr19 7.77 Mbp_chr19_7765326_F TCGTCGGCAGCGTCAGGTTCTGAGGGTCTGGGG 60.2 0.23
mm4__chr19 7.77 Mbp_chr19_7765326_R GTCTCGTGGGCTCGGCCAGGGACAACATCTTGGCT 59.9 0.23
mm4__chr15 31.81 Mbp_chr15_31813009_F TCGTCGGCAGCGTCATGGATTCTCAAGGCCAGGC 60.1 0.22
mm4__chr15 31.81 Mbp_chr15_31813009_R GTCTCGTGGGCTCGGCGCATCAGGTGTCTTGCTTC 59.5 0.22
mm4__chr6 142.47 Mbp_chr6_142473649_F Primer3: not found at this Tm N.d. 0.21
mm4__chr6 142.47 Mbp_chr6_142473649_R Primer3: not found at this Tm N.d. 0.21
mm4__chr1 109.49 Mbp_chr1_109491659_F Primer3: not found at this Tm N.d. 0.21
mm4__chr1 109.49 Mbp_chr1_109491659_R Primer3: not found at this Tm N.d. 0.21
mm4__chr16 67.54 Mbp_chr16_67537507_F Primer3: not found at this Tm N.d. 0.21
mm4__chr16 67.54 Mbp_chr16_67537507_R Primer3: not found at this Tm N.d. 0.21
mm3__chr2 69.99 Mbp_chr2_69989558_F TCGTCGGCAGCGTCCTGAGCCCTTGGACCTGC 60.0 0.21
mm3__chr2 69.99 Mbp_chr2_69989558_R GTCTCGTGGGCTCGGGCATCTTGTTTTCCCGTGCT 59.4 0.21
mm4__chr4 1.80 Mbp_chr4_1800075_F TCGTCGGCAGCGTCTGTTGCTGATGGGGAGACTG 59.6 0.20
mm4__chr4 1.80 Mbp_chr4_1800075_R GTCTCGTGGGCTCGGCGGGGTCCACCTCACCTATA 60.1 0.20
mm4__chr4 3.24 Mbp_chr4_3242546_F TCGTCGGCAGCGTCCTTTCCTTAGACCCGCCAGG 60.1 0.20
mm4__chr4 3.24 Mbp_chr4_3242546_R GTCTCGTGGGCTCGGCTCCCTTCTGCAGAGAGCTG 59.8 0.20
mm4__chr21 39.57 Mbp_chr21_39569416_F Primer3: not found at this Tm N.d. 0.20
mm4__chr21 39.57 Mbp_chr21_39569416_R Primer3: not found at this Tm N.d. 0.20
mm4__chr20 32.99 Mbp_chr20_32985583_F TCGTCGGCAGCGTCATCCCCTACCCTTGAAGGCT 59.9 0.19
mm4__chr20 32.99 Mbp_chr20_32985583_R GTCTCGTGGGCTCGGGTCTGCACAACACTTGCAGG 59.9 0.19
mm4__chr19 29.01 Mbp_chr19_29006859_F Primer3: not found at this Tm N.d. 0.18
mm4__chr19 29.01 Mbp_chr19_29006859_R Primer3: not found at this Tm N.d. 0.18
mm4__chr4 152.54 Mbp_chr4_152536214_F Primer3: not found at this Tm N.d. 0.18
mm4__chr4 152.54 Mbp_chr4_152536214_R Primer3: not found at this Tm N.d. 0.18
mm4__chr14 53.36 Mbp_chr14_53355727_F TCGTCGGCAGCGTCGTGTGAAGGAAACCACAGCAG 59.6 0.18
mm4__chr14 53.36 Mbp_chr14_53355727_R GTCTCGTGGGCTCGGACCAGTCTGGACGTGGAAAT 58.9 0.18
mm4__chr11 131.46 Mbp_chr11_131463880_F TCGTCGGCAGCGTCAGGAAGGAGAGAGGATGGGG 59.7 0.18
mm4__chr11 131.46 Mbp_chr11_131463880_R GTCTCGTGGGCTCGGCCAGTCCCGTTTCTCACACA 59.8 0.18
mm4__chr19 9.85 Mbp_chr19_9847288_F TCGTCGGCAGCGTCCACTTGGCTTCCGAAACGTG 60.0 0.18
mm4__chr19 9.85 Mbp_chr19_9847288_R GTCTCGTGGGCTCGGCATCGGCTCTGACTCTCCAG 59.6 0.18
mm4__chr16 71.41 Mbp_chr16_71410050_F Primer3: not found at this Tm N.d. 0.18
mm4__chr16 71.41 Mbp_chr16_71410050_R Primer3: not found at this Tm N.d. 0.18
mm4__chr19 10.01 Mbp_chr19_10010513_F Primer3: not found at this Tm N.d. 0.18
mm4__chr19 10.01 Mbp_chr19_10010513_R Primer3: not found at this Tm N.d. 0.18
mm4__chr3 194.27 Mbp_chr3_194268138_F TCGTCGGCAGCGTCACAGGCCCCTTTCTTCTCAC 59.5 0.17
mm4__chr3 194.27 Mbp_chr3_194268138_R GTCTCGTGGGCTCGGCTGAGAGGCTGAGTCACACG 60.1 0.17
mm4__chr17 30.70 Mbp_chr17_30697310_F TCGTCGGCAGCGTCCCCTGCCTCAGTTTACCTGT 59.3 0.17
mm4__chr17 30.70 Mbp_chr17_30697310_R GTCTCGTGGGCTCGGTCAGGGCCAGGATTCATTGT 58.9 0.17
mm4__chr15 77.03 Mbp_chr15_77034247_F TCGTCGGCAGCGTCCAGCACAATGGCTCAGCAAG 60.1 0.17
mm4__chr15 77.03 Mbp_chr15_77034247_R GTCTCGTGGGCTCGGAGCAAAGGACAGGGTGCAAG 60.8 0.17
mm4__chr11 76.65 Mbp_chr11_76653181_F TCGTCGGCAGCGTCAGGGCAGTCAAGTCACCAAG 59.8 0.16
mm4__chr11 76.65 Mbp_chr11_76653181_R GTCTCGTGGGCTCGGGGAGAATGGGGCCTGAACTC 60.1 0.16
mm4__chr13 113.08 Mbp_chr13_113076882_F TCGTCGGCAGCGTCCATGGAGCTGCCCTTTCCC 60.7 0.16
mm4__chr13 113.08 Mbp_chr13_113076882_R GTCTCGTGGGCTCGGAAATGTTCTCCATGCGGGGT 59.9 0.16
mm4__chr1 155.01 Mbp_chr1_155005757_F TCGTCGGCAGCGTCTGGGGACAGGCAGATGGATA 60.0 0.16
mm4__chr1 155.01 Mbp_chr1_155005757_R GTCTCGTGGGCTCGGACTTCAAACTGTCCGGGCAA 60.1 0.16
mm4__chr10 68.83 Mbp_chr10_68827191_F TCGTCGGCAGCGTCTAACTTGGAACGGCCCCAAA 59.8 0.16
mm4__chr10 68.83 Mbp_chr10_68827191_R GTCTCGTGGGCTCGGTTTTCCAAGTCCTGGGGCTC 59.8 0.16
mm4__chr1 201.52 Mbp_chr1_201516894_F TCGTCGGCAGCGTCACAGAGAGGCCATACCATGA 57.8 0.16
mm4__chr1 201.52 Mbp_chr1_201516894_R GTCTCGTGGGCTCGGTGTCTATGTGGGCTTCCAGC 59.7 0.16
mm4__chr19 18.76 Mbp_chr19_18761188_F TCGTCGGCAGCGTCGAGCTAGTCCCCTTGGCAAG 60.1 0.16
mm4__chr19 18.76 Mbp_chr19_18761188_R GTCTCGTGGGCTCGGCAACAGCTAAGGACGGAGCA 60.0 0.16
mm4__chr3 57.76 Mbp_chr3_57756918_F Primer3: not found at this Tm N.d. 0.16
mm4__chr3 57.76 Mbp_chr3_57756918_R Primer3: not found at this Tm N.d. 0.16
mm4__chr18 79.44 Mbp_chr18_79444532_F TCGTCGGCAGCGTCCTCGCTGCTCTGGAGGGA 60.7 0.16
mm4__chr18 79.44 Mbp_chr18_79444532_R GTCTCGTGGGCTCGGCCAGACCTGGCTTTAAGGGG 60.0 0.16
mm4__chr16 75.25 Mbp_chr16_75250781_F TCGTCGGCAGCGTCCGACCCTGCTCTAGCTCTTG 59.8 0.16
mm4__chr16 75.25 Mbp_chr16_75250781_R GTCTCGTGGGCTCGGCTGGCCACAGGAGTCCTAGC 61.9 0.16
mm4__chr16 12.25 Mbp_chr16_12248235_F Primer3: not found at this Tm N.d. 0.15
mm4__chr16 12.25 Mbp_chr16_12248235_R Primer3: not found at this Tm N.d. 0.15
mm4__chr14 100.50 Mbp_chr14_100503684_F TCGTCGGCAGCGTCCCCCGGCTTCACTTACACAT 60.0 0.15
mm4__chr14 100.50 Mbp_chr14_100503684_R GTCTCGTGGGCTCGGCAACGGGAATCCAGCAGTCT 60.0 0.15
mm4__chr11 67.63 Mbp_chr11_67631954_F Primer3: not found at this Tm N.d. 0.15
mm4__chr11 67.63 Mbp_chr11_67631954_R Primer3: not found at this Tm N.d. 0.15
mm4__chr15 41.54 Mbp_chr15_41536572_F TCGTCGGCAGCGTCGATCATGCCTCCTCAGCCTC 59.9 0.15
mm4__chr15 41.54 Mbp_chr15_41536572_R GTCTCGTGGGCTCGGGCTTTGGTCCCTTCTCCTCC 60.0 0.15
mm4__chr15 60.91 Mbp_chr15_60906678_F TCGTCGGCAGCGTCGCCAGACTGTATCAGAGGCA 59.1 0.15
mm4__chr15 60.91 Mbp_chr15_60906678_R GTCTCGTGGGCTCGGCGTGGCCGTAGTATCCATCC 60.0 0.15
mm4__chr12 6.38 Mbp_chr12_6384200_F TCGTCGGCAGCGTCCACTCCCACTTCCTGAGCTC 59.7 0.15
mm4__chr12 6.38 Mbp_chr12_6384200_R GTCTCGTGGGCTCGGGGACTCAGGGCCGATGTG 59.8 0.15
mm4__chr8 52.54 Mbp_chr8_52542586_F Primer3: not found at this Tm N.d. 0.15
mm4__chr8 52.54 Mbp_chr8_52542586_R Primer3: not found at this Tm N.d. 0.15
mm4__chr13 113.31 Mbp_chr13_113313477_F TCGTCGGCAGCGTCGATCCTGGACGTTGGTTCCT 59.3 0.15
mm4__chr13 113.31 Mbp_chr13_113313477_R GTCTCGTGGGCTCGGACACTGGCATCTCCACACTG 59.9 0.15
mm4__chr6 31.93 Mbp_chr6_31933901_F TCGTCGGCAGCGTCCTGACTCCTGTGTGGCTCTC 59.7 0.14
mm4__chr6 31.93 Mbp_chr6_31933901_R GTCTCGTGGGCTCGGCCCTTTGTCTTCTGGGTGGG 60.2 0.14
mm4__chrX 38.80 Mbp_chrX_38804040_F TCGTCGGCAGCGTCGTCCAGCTGCTCCAATCCC 60.4 0.14
mm4__chrX 38.80 Mbp_chrX_38804040_R GTCTCGTGGGCTCGGCTCCGCCTCTCGCCTGATA 60.8 0.14
mm3__chr11 44.57 Mbp_chr11_44565349_F TCGTCGGCAGCGTCGGAGGACGAGGTAGGCGG 61.2 0.14
mm3__chr11 44.57 Mbp_chr11_44565349_R GTCTCGTGGGCTCGGTCTCTCCTCTATCCCGGCAG 59.8 0.14
mm4__chr11 57.44 Mbp_chr11_57440081_F TCGTCGGCAGCGTCCGAGAGCCCTGGCACTAAAT 59.8 0.14
mm4__chr11 57.44 Mbp_chr11_57440081_R GTCTCGTGGGCTCGGGGGAAAACAAGTGGCTCTGC 59.6 0.14
mm4__chr4 76.21 Mbp_chr4_76214021_F TCGTCGGCAGCGTCGGTGGCCCTGAGTGGCTG 62.7 0.13
mm4__chr4 76.21 Mbp_chr4_76214021_R GTCTCGTGGGCTCGGCAGGTTCGGCAATTTCGGTG 60.1 0.13
mm4__chr5 139.80 Mbp_chr5_139796917_F Primer3: not found at this Tm N.d. 0.13
mm4__chr5 139.80 Mbp_chr5_139796917_R Primer3: not found at this Tm N.d. 0.13
mm4__chr7 1.90 Mbp_chr7_1898444_F TCGTCGGCAGCGTCGCACTCTCCACCTGCTTCTT 59.9 0.13
mm4__chr7 1.90 Mbp_chr7_1898444_R GTCTCGTGGGCTCGGCACCTGTACTCGGCTTCCTC 59.8 0.13
mm4__chr22 50.27 Mbp_chr22_50266457_F TCGTCGGCAGCGTCGGCCAGCCCAAAGTAGCATA 60.1 0.13
mm4__chr22 50.27 Mbp_chr22_50266457_R GTCTCGTGGGCTCGGGACTGTATCCTTGGCCCAGG 59.8 0.13
mm4__chr2 170.82 Mbp_chr2_170820044_F TCGTCGGCAGCGTCAGCATATGAACCAGGCACCG 60.4 0.13
mm4__chr2 170.82 Mbp_chr2_170820044_R GTCTCGTGGGCTCGGGCAAAAGGGAGCGGGAAATG 60.1 0.13
mm4__chr12 125.28 Mbp_chr12_125281222_F TCGTCGGCAGCGTCTATGCAAATGCGGAGTGGGT 60.0 0.13
mm4__chr12 125.28 Mbp_chr12_125281222_R GTCTCGTGGGCTCGGACACAGGTTCCTCAATCGCC 60.3 0.13
mm4__chr14 61.28 Mbp_chr14_61280889_F TCGTCGGCAGCGTCGGCAGCGAGAAGTACCAGG 60.1 0.13
mm4__chr14 61.28 Mbp_chr14_61280889_R GTCTCGTGGGCTCGGACTCCTCCAACGGCATCAAG 60.0 0.13
mm4__chr9 134.27 Mbp_chr9_134272811_F Primer3: not found at this Tm N.d. 0.13
mm4__chr9 134.27 Mbp_chr9_134272811_R Primer3: not found at this Tm N.d. 0.13
mm4__chr11 62.04 Mbp_chr11_62042408_F TCGTCGGCAGCGTCGTAAAGGCAGGTGGGGTGAG 60.3 0.13
mm4__chr11 62.04 Mbp_chr11_62042408_R GTCTCGTGGGCTCGGTCCAGCTGGCATTATAGGGC 59.5 0.13
mm4__chr12 132.20 Mbp_chr12_132202715_F TCGTCGGCAGCGTCATGAGCCCTCCCAGAACTCA 60.2 0.13
mm4__chr12 132.20 Mbp_chr12_132202715_R GTCTCGTGGGCTCGGCCCACAACCCTAAGAAAGGCA 60.2 0.13
mm4__chr2 219.04 Mbp_chr2_219041421_F TCGTCGGCAGCGTCCTCTCTGAAGGGCATGGAGG 59.5 0.12
mm4__chr2 219.04 Mbp_chr2_219041421_R GTCTCGTGGGCTCGGGAGCGTTAGTCCCAAGGTCC 60.1 0.12
mm4__chr1 43.37 Mbp_chr1_43368199_F TCGTCGGCAGCGTCTTCCCACCCCTTCCCTCG 60.6 0.12
mm4__chr1 43.37 Mbp_chr1_43368199_R GTCTCGTGGGCTCGGGAAGAGACAGCGTGCTCCTG 60.7 0.12
mm4__chr17 2.95 Mbp_chr17_2947448_F TCGTCGGCAGCGTCATAGCAGTAACACTGGCCCG 59.8 0.12
mm4__chr17 2.95 Mbp_chr17_2947448_R GTCTCGTGGGCTCGGAACGAAGCAAGCGGTGAGG 60.9 0.12
mm4__chrX 134.46 Mbp_chrX_134460355_F Primer3: not found at this Tm N.d. 0.12
mm4__chrX 134.46 Mbp_chrX_134460355_R Primer3: not found at this Tm N.d. 0.12
mm4__chr6 41.54 Mbp_chr6_41536436_F Primer3: not found at this Tm N.d. 0.12
mm4__chr6 41.54 Mbp_chr6_41536436_R Primer3: not found at this Tm N.d. 0.12
mm4__chr19 58.06 Mbp_chr19_58057136_F Primer3: not found at this Tm N.d. 0.12
mm4__chr19 58.06 Mbp_chr19_58057136_R Primer3: not found at this Tm N.d. 0.12
mm4__chr17 46.02 Mbp_chr17_46021005_F TCGTCGGCAGCGTCCCACCAGGCCACTCAAGTAG 60.0 0.12
mm4__chr17 46.02 Mbp_chr17_46021005_R GTCTCGTGGGCTCGGGGGTGCCCATCTGTTCAGAA 59.9 0.12
mm4__chr3 48.66 Mbp_chr3_48664006_F TCGTCGGCAGCGTCCCTCCAAAGGCGGAATCGAT 60.1 0.12
mm4__chr3 48.66 Mbp_chr3_48664006_R GTCTCGTGGGCTCGGGAACCAGGATCCACCCCATC 59.8 0.12
mm4__chr16 55.64 Mbp_chr16_55636355_F TCGTCGGCAGCGTCTCATCAGGTCAGTGCTGGTG 59.6 0.11
mm4__chr16 55.64 Mbp_chr16_55636355_R GTCTCGTGGGCTCGGGCCTCAGGCTCCTTACTTCC 59.8 0.11
mm4__chr8 28.70 Mbp_chr8_28701459_F TCGTCGGCAGCGTCGACGCGGTCGGGAAAGTG 61.1 0.11
mm4__chr8 28.70 Mbp_chr8_28701459_R GTCTCGTGGGCTCGGCCATTTCCGACAGGCGACT 60.0 0.11
mm4__chr2 5.35 Mbp_chr2_5348964_F TCGTCGGCAGCGTCGAGCATGAGCAGGTGTGGAG 60.7 0.11
mm4__chr2 5.35 Mbp_chr2_5348964_R GTCTCGTGGGCTCGGCTCGTGCTGCTTGGCTTCT 60.6 0.11
mm4__chr9 135.61 Mbp_chr9_135612567_F TCGTCGGCAGCGTCGATGGAGGCCAGAAGCTGTC 60.4 0.11
mm4__chr9 135.61 Mbp_chr9_135612567_R GTCTCGTGGGCTCGGCATCCTGCATCCTGCCTACA 59.5 0.11
mm4__chr1 93.60 Mbp_chr1_93599598_F TCGTCGGCAGCGTCCTGGCTGCAGTGAGAGCAAT 60.6 0.10
mm4__chr1 93.60 Mbp_chr1_93599598_R GTCTCGTGGGCTCGGAAAGACTCTGACCTGTGCCT 58.5 0.10
mm4__chr8 46.22 Mbp_chr8_46223629_F Primer3: not found at this Tm N.d. 0.10
mm4__chr8 46.22 Mbp_chr8_46223629_R Primer3: not found at this Tm N.d. 0.10
mm4__chr5 139.80 Mbp_chr5_139795658_F Primer3: not found at this Tm N.d. 0.10
mm4__chr5 139.80 Mbp_chr5_139795658_R Primer3: not found at this Tm N.d. 0.10
mm4__chr4 152.09 Mbp_chr4_152090667_F TCGTCGGCAGCGTCCACAAGCACATTGGCGGTTG 60.9 0.10
mm4__chr4 152.09 Mbp_chr4_152090667_R GTCTCGTGGGCTCGGACTCACCCCAGGCCTATCTT 59.9 0.10
mm4__chr8 100.89 Mbp_chr8_100888452_F TCGTCGGCAGCGTCGTGGGCTTACCTGCTGATGT 60.0 0.10
mm4__chr8 100.89 Mbp_chr8_100888452_R GTCTCGTGGGCTCGGGACCGAGCAGATGAGAGTGG 59.8 0.10
mm4__chr16 81.28 Mbp_chr16_81276462_F Primer3: not found at this Tm N.d. 0.09
mm4__chr16 81.28 Mbp_chr16_81276462_R Primer3: not found at this Tm N.d. 0.09
mm4__chr9 135.82 Mbp_chr9_135820450_F Primer3: not found at this Tm N.d. 0.09
mm4__chr9 135.82 Mbp_chr9_135820450_R Primer3: not found at this Tm N.d. 0.09
mm4__chr1 56.53 Mbp_chr1_56525642_F TCGTCGGCAGCGTCACCCTGCTGCCTTTGTCTTA 59.2 0.09
mm4__chr1 56.53 Mbp_chr1_56525642_R GTCTCGTGGGCTCGGCCATCACTGTCTCTGGGCTG 60.1 0.09
mm4__chr1 90.19 Mbp_chr1_90190060_F Primer3: not found at this Tm N.d. 0.09
mm4__chr1 90.19 Mbp_chr1_90190060_R Primer3: not found at this Tm N.d. 0.09
mm4__chr2 241.95 Mbp_chr2_241953837_F TCGTCGGCAGCGTCCAAAGGCCTGGAGCAGATGT 60.3 0.09
mm4__chr2 241.95 Mbp_chr2_241953837_R GTCTCGTGGGCTCGGAGCGGAGACCTCAGGACAG 60.6 0.09
mm4__chr6 155.64 Mbp_chr6_155636783_F TCGTCGGCAGCGTCATGCTGTGCTGCTCATTCCT 60.0 0.08
mm4__chr6 155.64 Mbp_chr6_155636783_R GTCTCGTGGGCTCGGTGTGAAGTGTCAGGGTGTGG 59.8 0.08
mm4__chr1 11.52 Mbp_chr1_11522393_F TCGTCGGCAGCGTCGCGTGATAATCCACTGCAGC 59.6 0.08
mm4__chr1 11.52 Mbp_chr1_11522393_R GTCTCGTGGGCTCGGCGGTGATGATGGGGAGCAAT 60.1 0.08
mm4__chr1 202.02 Mbp_chr1_202018969_F Primer3: not found at this Tm N.d. 0.08
mm4__chr1 202.02 Mbp_chr1_202018969_R Primer3: not found at this Tm N.d. 0.08
mm4__chr12 52.06 Mbp_chr12_52061120_F TCGTCGGCAGCGTCGACAGACGTGAGGACAGGAC 59.7 0.08
mm4__chr12 52.06 Mbp_chr12_52061120_R GTCTCGTGGGCTCGGGAACCCTAATACCCGCCAGC 60.5 0.08
mm4__chr16 74.71 Mbp_chr16_74714135_F TCGTCGGCAGCGTCTAATGGGGTCTGAATGGCGG 59.8 0.08
mm4__chr16 74.71 Mbp_chr16_74714135_R GTCTCGTGGGCTCGGCACTCCAGAGAAACCCCACC 59.9 0.08
mm4__chr16 2.96 Mbp_chr16_2956399_F TCGTCGGCAGCGTCTGCAGACATTTTGGAGGCCA 60.1 0.08
mm4__chr16 2.96 Mbp_chr16_2956399_R GTCTCGTGGGCTCGGCGCCCTTCTCAGATGAGGAC 59.8 0.08
mm3__chr19 518.94 Kbp_chr19_518942_F TCGTCGGCAGCGTCCGCCTTCAACAACAACGTGA 59.6 0.08
mm3__chr19 518.94 Kbp_chr19_518942_R GTCTCGTGGGCTCGGGCAGCTCGCTGTAGGTGC 61.1 0.08
mm4__chr17 28.52 Mbp_chr17_28524635_F TCGTCGGCAGCGTCGAGCAAGTCCAGGGCCAC 60.3 0.08
mm4__chr17 28.52 Mbp_chr17_28524635_R GTCTCGTGGGCTCGGCCTCAAAGCCAAAGCCAGGA 60.5 0.08
mm4__chr14 88.79 Mbp_chr14_88792858_F Primer3: not found at this Tm N.d. 0.08
mm4__chr14 88.79 Mbp_chr14_88792858_R Primer3: not found at this Tm N.d. 0.08
mm4__chr17 39.24 Mbp_chr17_39238768_F Primer3: not found at this Tm N.d. 0.08
mm4__chr17 39.24 Mbp_chr17_39238768_R Primer3: not found at this Tm N.d. 0.08
mm4__chr2 219.61 Mbp_chr2_219612737_F Primer3: not found at this Tm N.d. 0.08
mm4__chr2 219.61 Mbp_chr2_219612737_R Primer3: not found at this Tm N.d. 0.08
mm4__chr1 1.18 Mbp_chr1_1182573_F TCGTCGGCAGCGTCTGAGGGCTGGACCAAGGC 61.6 0.08
mm4__chr1 1.18 Mbp_chr1_1182573_R GTCTCGTGGGCTCGGCCCCTTTCCAACTCCTCAGT 58.9 0.08
mm4__chr9 136.52 Mbp_chr9_136517726_F TCGTCGGCAGCGTCAGCAACCCACAGATGTTCCC 60.2 0.07
mm4__chr9 136.52 Mbp_chr9_136517726_R GTCTCGTGGGCTCGGGGAGTTCCAGTGCATCTGCA 60.3 0.07
mm4__chr11 131.25 Mbp_chr11_131254449_F TCGTCGGCAGCGTCGAGAGAAGAGTCGCTGAGCC 59.8 0.07
mm4__chr11 131.25 Mbp_chr11_131254449_R GTCTCGTGGGCTCGGCTGGGATCAGAACCTCAGGC 59.8 0.07
mm4__chr7 157.43 Mbp_chr7_157428886_F TCGTCGGCAGCGTCCCTCTGGAGGGACCTGGATT 60.3 0.07
mm4__chr7 157.43 Mbp_chr7_157428886_R GTCTCGTGGGCTCGGGTGGGCAGATTTCCCAGTGA 59.9 0.07
mm4__chr15 46.18 Mbp_chr15_46183382_F Primer3: not found at this Tm N.d. 0.07
mm4__chr15 46.18 Mbp_chr15_46183382_R Primer3: not found at this Tm N.d. 0.07
mm4__chr19 2.23 Mbp_chr19_2229414_F TCGTCGGCAGCGTCCAAGGAGAGGAGGACTGGGA 59.9 0.07
mm4__chr19 2.23 Mbp_chr19_2229414_R GTCTCGTGGGCTCGGCACCCTACCTGCAGCTCATC 60.1 0.07
mm4__chr1 1.44 Mbp_chr1_1440125_F TCGTCGGCAGCGTCCGTCAGACCCAGGACTGTTC 60.0 0.07
mm4__chr1 1.44 Mbp_chr1_1440125_R GTCTCGTGGGCTCGGTCCCCTTCCGCTTCCTATCA 60.0 0.07
mm3__chr8 139.98 Mbp_chr8_139982937_F TCGTCGGCAGCGTCTTCACACTTACCACCCCACC 59.5 0.07
mm3__chr8 139.98 Mbp_chr8_139982937_R GTCTCGTGGGCTCGGTTTACAGCACGACGTGACGA 59.9 0.07
mm4__chrX 107.98 Mbp_chrX_107981494_F TCGTCGGCAGCGTCCTCACCTGCTCCTTCTGTCC 59.7 0.06
mm4__chrX 107.98 Mbp_chrX_107981494_R GTCTCGTGGGCTCGGCCCTGTCCCCAGAATGTGTT 59.5 0.06
mm4__chrX 105.22 Mbp_chrX_105219693_F TCGTCGGCAGCGTCGCTGCTCCTTCTGTGCAAAC 60.0 0.06
mm4__chrX 105.22 Mbp_chrX_105219693_R GTCTCGTGGGCTCGGCTGGTGTCTCCCCATGAGTG 59.7 0.06
mm4__chr18 70.29 Mbp_chr18_70289294_F Primer3: not found at this Tm N.d. 0.06
mm4__chr18 70.29 Mbp_chr18_70289294_R Primer3: not found at this Tm N.d. 0.06
mm4__chr13 110.17 Mbp_chr13_110167504_F Primer3: not found at this Tm N.d. 0.06
mm4__chr13 110.17 Mbp_chr13_110167504_R Primer3: not found at this Tm N.d. 0.06
mm4__chr3 46.68 Mbp_chr3_46680238_F Primer3: not found at this Tm N.d. 0.06
mm4__chr3 46.68 Mbp_chr3_46680238_R Primer3: not found at this Tm N.d. 0.06
mm3__chr7 30.85 Mbp_chr7_30854026_F TCGTCGGCAGCGTCCTGTACATCCACTCCTGGCC 59.8 0.06
mm3__chr7 30.85 Mbp_chr7_30854026_R GTCTCGTGGGCTCGGACAGCTCTGCCATGACTTCC 60.0 0.06
mm4__chr20 17.71 Mbp_chr20_17710175_F Primer3: not found at this Tm N.d. 0.05
mm4__chr20 17.71 Mbp_chr20_17710175_R Primer3: not found at this Tm N.d. 0.05
mm4__chr5 177.13 Mbp_chr5_177133778_F TCGTCGGCAGCGTCGGGTGTGAGGAGGAGGAGG 60.6 0.05
mm4__chr5 177.13 Mbp_chr5_177133778_R GTCTCGTGGGCTCGGTCTCTTCAGGGGAGAAGGCA 59.8 0.05
mm4__chr7 47.70 Mbp_chr7_47698361_F TCGTCGGCAGCGTCGCAGTGTGGTGGAGGAAACA 60.4 0.05
mm4__chr7 47.70 Mbp_chr7_47698361_R GTCTCGTGGGCTCGGGCCCTTCTGTCTTCCCACTC 60.0 0.05
mm4__chr10 691.18 Kbp_chr10_691185_F TCGTCGGCAGCGTCACCCCAGGTCCAGAACAATC 59.3 0.05
mm4__chr10 691.18 Kbp_chr10_691185_R GTCTCGTGGGCTCGGCCAGGCGCTTACACTCACAA 60.6 0.05
mm4__chr5 173.79 Mbp_chr5_173785985_F TCGTCGGCAGCGTCTCACCTTCCTCCCCTTTCAGA 60.1 0.05
mm4__chr5 173.79 Mbp_chr5_173785985_R GTCTCGTGGGCTCGGCCCAGCAGAGGGTGTAGACA 60.9 0.05
mm3__chr11 66.90 Mbp_chr11_66902672_F TCGTCGGCAGCGTCCAACCTCAGCCACTCTCCTC 59.7 0.05
mm3__chr11 66.90 Mbp_chr11_66902672_R GTCTCGTGGGCTCGGAGGGCTGTGGTCAGAGTTTG 59.8 0.05
mm4__chr1 16.14 Mbp_chr1_16139301_F TCGTCGGCAGCGTCCCCTACAGGCAGGAAGCTG 59.7 0.05
mm4__chr1 16.14 Mbp_chr1_16139301_R GTCTCGTGGGCTCGGGTAGGGTTGTTGGAGGAGCTC 60.0 0.05
mm4__chr9 117.41 Mbp_chr9_117414921_F Primer3: not found at this Tm N.d. 0.04
mm4__chr9 117.41 Mbp_chr9_117414921_R Primer3: not found at this Tm N.d. 0.04
mm2__chr3 149.75 Mbp_chr3_149752480_F TCGTCGGCAGCGTCTGTCCTACGATTTCACCCGG 59.4 0.04
mm2__chr3 149.75 Mbp_chr3_149752480_R GTCTCGTGGGCTCGGCGTCCGAGTGTAACGCGTTA 60.4 0.04
mm4__chr15 28.89 Mbp_chr15_28885991_F Primer3: not found at this Tm N.d. 0.04
mm4__chr15 28.89 Mbp_chr15_28885991_R Primer3: not found at this Tm N.d. 0.04
mm4__chr16 11.49 Mbp_chr16_11494715_F TCGTCGGCAGCGTCCGTCCAGAGAAGCACAGAGG 60.1 0.04
mm4__chr16 11.49 Mbp_chr16_11494715_R GTCTCGTGGGCTCGGATTTCTGTCCCCAGCCACTG 59.9 0.04
mm3__chr17 74.92 Mbp_chr17_74923074_F TCGTCGGCAGCGTCCGGTGGTACTGGTCGTTCAT 59.7 0.04
mm3__chr17 74.92 Mbp_chr17_74923074_R GTCTCGTGGGCTCGGCCTCCAACCTCATGCCTCAG 60.1 0.04
mm4__chr20 38.16 Mbp_chr20_38159011_F TCGTCGGCAGCGTCAGAGATCTGGGCTCTTCCGT 60.0 0.04
mm4__chr20 38.16 Mbp_chr20_38159011_R GTCTCGTGGGCTCGGTGTCCTCCAGTCTCCACCAA 60.1 0.04
mm4__chr14 72.81 Mbp_chr14_72809324_F TCGTCGGCAGCGTCCATCATCGTCACACAGGGCT 60.1 0.04
mm4__chr14 72.81 Mbp_chr14_72809324_R GTCTCGTGGGCTCGGTGCCATGTTCCCCAAGGTC 59.9 0.04
mm4__chr7 84.94 Mbp_chr7_84940225_F Primer3: not found at this Tm N.d. 0.04
mm4__chr7 84.94 Mbp_chr7_84940225_R Primer3: not found at this Tm N.d. 0.04
mm4__chr10 43.26 Mbp_chr10_43263687_F TCGTCGGCAGCGTCGCTCACAAGAGACAAGCAGG 58.8 0.04
mm4__chr10 43.26 Mbp_chr10_43263687_R GTCTCGTGGGCTCGGGTTCTGGGAAGCTTCTCCCC 60.0 0.04
mm4__chr8 133.82 Mbp_chr8_133818162_F Primer3: not found at this Tm N.d. 0.04
mm4__chr8 133.82 Mbp_chr8_133818162_R Primer3: not found at this Tm N.d. 0.04
mm4__chr11 69.88 Mbp_chr11_69879886_F TCGTCGGCAGCGTCCAGGCCTCTTTGGAGAGCAA 59.9 0.03
mm4__chr11 69.88 Mbp_chr11_69879886_R GTCTCGTGGGCTCGGTTTCTCAAAGGCCTGCCCTC 60.2 0.03
mm4__chr10 114.77 Mbp_chr10_114768156_F Primer3: not found at this Tm N.d. 0.03
mm4__chr10 114.77 Mbp_chr10_114768156_R Primer3: not found at this Tm N.d. 0.03
mm4__chr6 6.92 Mbp_chr6_6920277_F Primer3: not found at this Tm N.d. 0.03
mm4__chr6 6.92 Mbp_chr6_6920277_R Primer3: not found at this Tm N.d. 0.03
mm4__chr10 124.09 Mbp_chr10_124093337_F Primer3: not found at this Tm N.d. 0.03
mm4__chr10 124.09 Mbp_chr10_124093337_R Primer3: not found at this Tm N.d. 0.03
mm3__chr12 54.47 Mbp_chr12_54473594_F TCGTCGGCAGCGTCCACAGTCACCTTCCTCCCAC 59.9 0.03
mm3__chr12 54.47 Mbp_chr12_54473594_R GTCTCGTGGGCTCGGCTCAGCCAGAACCGAGACTC 59.8 0.03
mm4__chr8 21.63 Mbp_chr8_21626032_F Primer3: not found at this Tm N.d. 0.03
mm4__chr8 21.63 Mbp_chr8_21626032_R Primer3: not found at this Tm N.d. 0.03
mm4__chr1 8.22 Mbp_chr1_8217374_F TCGTCGGCAGCGTCGTCAGCCAGGCCAGCTTG 61.0 0.03
mm4__chr1 8.22 Mbp_chr1_8217374_R GTCTCGTGGGCTCGGGCTGCGGGGAGAGAAGCC 62.8 0.03
mm4__chr19 53.25 Mbp_chr19_53254906_F TCGTCGGCAGCGTCAAGACGGAGACTCACCCGAG 60.9 0.03
mm4__chr19 53.25 Mbp_chr19_53254906_R GTCTCGTGGGCTCGGCCCATCCCCACAGTTGTCC 60.0 0.03
mm4__chr2 19.57 Mbp_chr2_19568880_F TCGTCGGCAGCGTCCACAACAAACTCCAGTCTGATGT 59.1 0.02
mm4__chr2 19.57 Mbp_chr2_19568880_R GTCTCGTGGGCTCGGCAACCCTCAATGGCCCTTGA 60.2 0.02
mm4__chr20 3.89 Mbp_chr20_3889778_F TCGTCGGCAGCGTCGTAAGTGTTCCGTGGGGCG 61.0 0.02
mm4__chr20 3.89 Mbp_chr20_3889778_R GTCTCGTGGGCTCGGCACCAACGAGGGACCACC 59.9 0.02
mm4__chr15 101.09 Mbp_chr15_101086434_F TCGTCGGCAGCGTCCAGCTACGTCACCTCCCAC 59.7 0.02
mm4__chr15 101.09 Mbp_chr15_101086434_R GTCTCGTGGGCTCGGGGTGACATAGCTGCAGGGG 60.1 0.02
mm4__chr11_KI270721v1_random 8.78 Kbp_chr11_KI270721v1_random_8783_F TCGTCGGCAGCGTCGCACTTTCTCTCTGGGCCAA 60.2 0.02
mm4__chr11_KI270721v1_random 8.78 Kbp_chr11_KI270721v1_random_8783_R GTCTCGTGGGCTCGGGGCCTCCAGGGACCATTTAC 60.1 0.02
mm4__chr3 13.22 Mbp_chr3_13220173_F Primer3: not found at this Tm N.d. 0.02
mm4__chr3 13.22 Mbp_chr3_13220173_R Primer3: not found at this Tm N.d. 0.02
mm3__chr9 125.52 Mbp_chr9_125515672_F TCGTCGGCAGCGTCTGAACCGATCACCAGAGATCA 58.5 0.02
mm3__chr9 125.52 Mbp_chr9_125515672_R GTCTCGTGGGCTCGGGTTTATTGCACCGACTGGCC 59.8 0.02
mm3__chr14 87.34 Mbp_chr14_87336313_F Primer3: not found at this Tm N.d. 0.02
mm3__chr14 87.34 Mbp_chr14_87336313_R Primer3: not found at this Tm N.d. 0.02
mm2__chr16 28.21 Mbp_chr16_28211954_F TCGTCGGCAGCGTCCCGACCAACAGCCCCAAC 60.9 0.02
mm2__chr16 28.21 Mbp_chr16_28211954_R GTCTCGTGGGCTCGGGGATTGGTCCGGTTCCAGC 60.7 0.02
mm4__chr6 36.84 Mbp_chr6_36840041_F Primer3: not found at this Tm N.d. 0.02
mm4__chr6 36.84 Mbp_chr6_36840041_R Primer3: not found at this Tm N.d. 0.02
mm4__chr8 42.33 Mbp_chr8_42331213_F Primer3: not found at this Tm N.d. 0.02
mm4__chr8 42.33 Mbp_chr8_42331213_R Primer3: not found at this Tm N.d. 0.02
mm3__chr11 2.41 Mbp_chr11_2409230_F TCGTCGGCAGCGTCTGAGTGTCTGTGTGTGCACT 59.4 0.02
mm3__chr11 2.41 Mbp_chr11_2409230_R GTCTCGTGGGCTCGGCATCAGTCTGCCCAGGAACA 59.6 0.02
mm4__chr4 8.79 Mbp_chr4_8791173_F Primer3: not found at this Tm N.d. 0.02
mm4__chr4 8.79 Mbp_chr4_8791173_R Primer3: not found at this Tm N.d. 0.02
mm3__chr20 40.33 Mbp_chr20_40326990_F TCGTCGGCAGCGTCACGGAGTCCAGTTACAAGGC 59.6 0.02
mm3__chr20 40.33 Mbp_chr20_40326990_R GTCTCGTGGGCTCGGATAGGATGGGGTGGAGGTCC 60.1 0.02
mm3__chr1 227.79 Mbp_chr1_227788017_F TCGTCGGCAGCGTCCCTCCCGGCTAGTTCCTGAG 61.3 0.01
mm3__chr1 227.79 Mbp_chr1_227788017_R GTCTCGTGGGCTCGGCCACACCCACAGCCCCTC 62.3 0.01
mm4__chr11_KI270721v1_random 5.18 Kbp_chr11_KI270721v1_random_5180_F TCGTCGGCAGCGTCGAGTTTCTCCTGTGCCCCTC 60.0 0.01
mm4__chr11_KI270721v1_random 5.18 Kbp_chr11_KI270721v1_random_5180_R GTCTCGTGGGCTCGGATTCTCCCACGTTCAGCTGG 60.0 0.01
mm4__chr11 1.95 Mbp_chr11_1949867_F TCGTCGGCAGCGTCGAGTTTCTCCTGTGCCCCTC 60.0 0.01
mm4__chr11 1.95 Mbp_chr11_1949867_R GTCTCGTGGGCTCGGATTCTCCCACGTTCAGCTGG 60.0 0.01
mm4__chr14 75.56 Mbp_chr14_75557006_F Primer3: not found at this Tm N.d. 0.01
mm4__chr14 75.56 Mbp_chr14_75557006_R Primer3: not found at this Tm N.d. 0.01
mm3__chr22 49.82 Mbp_chr22_49821092_F Primer3: not found at this Tm N.d. 0.01
mm3__chr22 49.82 Mbp_chr22_49821092_R Primer3: not found at this Tm N.d. 0.01
mm4__chr3 51.39 Mbp_chr3_51392901_F TCGTCGGCAGCGTCGGTTTTCCCTTGGGTGGACC 60.8 0.01
mm4__chr3 51.39 Mbp_chr3_51392901_R GTCTCGTGGGCTCGGGCCGGGTGTATCCATCTGTG 60.5 0.01
mm4__chr2 88.12 Mbp_chr2_88122514_F TCGTCGGCAGCGTCTCCTCTACCTTCTGCCCCTG 60.3 0.01
mm4__chr2 88.12 Mbp_chr2_88122514_R GTCTCGTGGGCTCGGGACACTGACCCGCCCAATAA 60.0 0.01
mm4__chr2 130.73 Mbp_chr2_130727814_F TCGTCGGCAGCGTCGATGATGGTGGTCCGGGG 59.4 0.01
mm4__chr2 130.73 Mbp_chr2_130727814_R GTCTCGTGGGCTCGGCTCGTCACAGCCCGAGAC 59.8 0.01
mm4__chr21 44.63 Mbp_chr21_44626421_F TCGTCGGCAGCGTCAAAGATGCTTGCAGTGCAGG 59.3 0.01
mm4__chr21 44.63 Mbp_chr21_44626421_R GTCTCGTGGGCTCGGCCCGTGAAGTAGCTGTGCTA 59.4 0.01
mm4__chr11 45.89 Mbp_chr11_45891093_F TCGTCGGCAGCGTCCTGGCTCCTGATGGACCTTG 60.1 0.00
mm4__chr11 45.89 Mbp_chr11_45891093_R GTCTCGTGGGCTCGGCCAGCAATCCAGAGACGAACT 60.0 0.00
mm4__chr9 134.65 Mbp_chr9_134654854_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 134.65 Mbp_chr9_134654854_R Primer3: not found at this Tm N.d. 0.00
mm3__chr17 40.94 Mbp_chr17_40936531_F TCGTCGGCAGCGTCGTGAAGGACAGGGAGATGCG 60.4 0.00
mm3__chr17 40.94 Mbp_chr17_40936531_R GTCTCGTGGGCTCGGAACTCCGGACACAGCTTCAG 59.9 0.00
mm4__chr1 155.13 Mbp_chr1_155127798_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 155.13 Mbp_chr1_155127798_R Primer3: not found at this Tm N.d. 0.00
mm3__chr15 42.14 Mbp_chr15_42142748_F Primer3: not found at this Tm N.d. 0.00
mm3__chr15 42.14 Mbp_chr15_42142748_R Primer3: not found at this Tm N.d. 0.00
mm4__chr20 2.17 Mbp_chr20_2172658_F TCGTCGGCAGCGTCCCTCTGCCACACTTGCAAAC 59.9 0.00
mm4__chr20 2.17 Mbp_chr20_2172658_R GTCTCGTGGGCTCGGCCCAATCTTTCCAGGAGGGG 59.7 0.00
mm4__chr15 80.06 Mbp_chr15_80059900_F Primer3: not found at this Tm N.d. 0.00
mm4__chr15 80.06 Mbp_chr15_80059900_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 110.03 Mbp_chr1_110034690_F TCGTCGGCAGCGTCGGCCCACTGATCGTGAACAA 60.6 0.00
mm4__chr1 110.03 Mbp_chr1_110034690_R GTCTCGTGGGCTCGGGTTGAACTCGCGGGCTTTG 60.0 0.00
mm4__chr7 47.98 Mbp_chr7_47979351_F TCGTCGGCAGCGTCCTTCATCCCCACAAGTGCCC 60.9 0.00
mm4__chr7 47.98 Mbp_chr7_47979351_R GTCTCGTGGGCTCGGGAGGATCAGAACGGGGATGG 59.6 0.00
mm4__chr6 165.86 Mbp_chr6_165855291_F TCGTCGGCAGCGTCAATGAACAGAGGCGGATGGG 60.1 0.00
mm4__chr6 165.86 Mbp_chr6_165855291_R GTCTCGTGGGCTCGGGCTACCAGGTGATCCACAGG 59.8 0.00
mm4__chr9 97.52 Mbp_chr9_97517576_F TCGTCGGCAGCGTCATGCTGGGTTGGGCATGTG 60.9 0.00
mm4__chr9 97.52 Mbp_chr9_97517576_R GTCTCGTGGGCTCGGGGGCCTGCTACCTGTGATAA 59.1 0.00
mm4__chr9 96.56 Mbp_chr9_96560216_F TCGTCGGCAGCGTCTTCACGCTGTAGTCCTCGTG 59.7 0.00
mm4__chr9 96.56 Mbp_chr9_96560216_R GTCTCGTGGGCTCGGAGAACTCCAAAGCAGAGGGG 59.3 0.00
mm4__chr9 128.20 Mbp_chr9_128203686_F Primer3: not found at this Tm N.d. 0.00
mm4__chr9 128.20 Mbp_chr9_128203686_R Primer3: not found at this Tm N.d. 0.00
mm4__chr8 93.74 Mbp_chr8_93740817_F TCGTCGGCAGCGTCCCGTTCTTCCGGCTTGGT 59.9 0.00
mm4__chr8 93.74 Mbp_chr8_93740817_R GTCTCGTGGGCTCGGCGAAGAGTTCCCTCAGCCTC 59.8 0.00
mm4__chr8 8.39 Mbp_chr8_8386175_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 8.39 Mbp_chr8_8386175_R Primer3: not found at this Tm N.d. 0.00
mm4__chr8 42.39 Mbp_chr8_42392084_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 42.39 Mbp_chr8_42392084_R Primer3: not found at this Tm N.d. 0.00
mm4__chr8 143.56 Mbp_chr8_143559971_F Primer3: not found at this Tm N.d. 0.00
mm4__chr8 143.56 Mbp_chr8_143559971_R Primer3: not found at this Tm N.d. 0.00
mm4__chr7 44.23 Mbp_chr7_44229411_F TCGTCGGCAGCGTCAGCAGGACCCAGGCTACTTA 59.9 0.00
mm4__chr7 44.23 Mbp_chr7_44229411_R GTCTCGTGGGCTCGGCCGACATCCTGAGCTCAGTG 60.1 0.00
mm4__chr7 1.24 Mbp_chr7_1236638_F Primer3: not found at this Tm N.d. 0.00
mm4__chr7 1.24 Mbp_chr7_1236638_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 75.45 Mbp_chr6_75450139_F Primer3: not found at this Tm N.d. 0.00
mm4__chr6 75.45 Mbp_chr6_75450139_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 33.82 Mbp_chr6_33823999_F Primer3: not found at this Tm N.d. 0.00
mm4__chr6 33.82 Mbp_chr6_33823999_R Primer3: not found at this Tm N.d. 0.00
mm4__chr6 169.70 Mbp_chr6_169702772_F TCGTCGGCAGCGTCCCAGCTTCGACGACTACGAG 60.2 0.00
mm4__chr6 169.70 Mbp_chr6_169702772_R GTCTCGTGGGCTCGGTGGGAGGGGTGTCCATAGAC 60.3 0.00
mm4__chr6 149.65 Mbp_chr6_149648669_F Primer3: not found at this Tm N.d. 0.00
mm4__chr6 149.65 Mbp_chr6_149648669_R Primer3: not found at this Tm N.d. 0.00
mm4__chr5 173.06 Mbp_chr5_173057267_F TCGTCGGCAGCGTCCGGATGGAGACTTCTGCACC 60.4 0.00
mm4__chr5 173.06 Mbp_chr5_173057267_R GTCTCGTGGGCTCGGCACTGGTCCCCTCCCATCTA 60.0 0.00
mm4__chr5 138.44 Mbp_chr5_138438780_F Primer3: not found at this Tm N.d. 0.00
mm4__chr5 138.44 Mbp_chr5_138438780_R Primer3: not found at this Tm N.d. 0.00
mm4__chr4 185.21 Mbp_chr4_185210287_F TCGTCGGCAGCGTCGTAAGTACCCGACTCCTGGC 59.5 0.00
mm4__chr4 185.21 Mbp_chr4_185210287_R GTCTCGTGGGCTCGGGGCTTCCCGCTTCGACAA 60.3 0.00
mm4__chr3 54.92 Mbp_chr3_54918787_F TCGTCGGCAGCGTCCTGCGAGGACACTGGATCAG 60.1 0.00
mm4__chr3 54.92 Mbp_chr3_54918787_R GTCTCGTGGGCTCGGGGGGACTAACAGACGGCATC 60.1 0.00
mm4__chr3 43.11 Mbp_chr3_43105614_F TCGTCGGCAGCGTCGAGGGCTCGAACTGGCAAG 60.7 0.00
mm4__chr3 43.11 Mbp_chr3_43105614_R GTCTCGTGGGCTCGGGTGAAGCGAGCTGAGGGAG 60.1 0.00
mm4__chr3 141.05 Mbp_chr3_141051227_F TCGTCGGCAGCGTCGGCTCCACTTCCCTCTCCC 61.3 0.00
mm4__chr3 141.05 Mbp_chr3_141051227_R GTCTCGTGGGCTCGGAATCCAATAGCCGCGGAAGG 60.5 0.00
mm4__chr3 120.35 Mbp_chr3_120349343_F TCGTCGGCAGCGTCGGAACCTGAACCGAGGGC 60.0 0.00
mm4__chr3 120.35 Mbp_chr3_120349343_R GTCTCGTGGGCTCGGCAAGGCGCGCAGGGTTTG 62.7 0.00
mm4__chr22 43.68 Mbp_chr22_43682072_F TCGTCGGCAGCGTCTTTCCCATGACTGCCCTGAG 59.6 0.00
mm4__chr22 43.68 Mbp_chr22_43682072_R GTCTCGTGGGCTCGGAGTGAGATTCCCCTCCCCTC 60.0 0.00
mm4__chr20 61.40 Mbp_chr20_61400835_F TCGTCGGCAGCGTCCCTCCTTGTTGTTCTGGCCT 59.8 0.00
mm4__chr20 61.40 Mbp_chr20_61400835_R GTCTCGTGGGCTCGGCTACAGTGGCAAAGGGACGT 59.9 0.00
mm4__chr2 71.46 Mbp_chr2_71461691_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 71.46 Mbp_chr2_71461691_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 29.92 Mbp_chr2_29920054_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 29.92 Mbp_chr2_29920054_R Primer3: not found at this Tm N.d. 0.00
mm4__chr2 238.80 Mbp_chr2_238796507_F TCGTCGGCAGCGTCCTGCAGGTCCCCTTCATGTC 60.3 0.00
mm4__chr2 238.80 Mbp_chr2_238796507_R GTCTCGTGGGCTCGGGGGCACAGAAGGGAAAGGTT 60.1 0.00
mm4__chr2 237.63 Mbp_chr2_237628052_F Primer3: not found at this Tm N.d. 0.00
mm4__chr2 237.63 Mbp_chr2_237628052_R Primer3: not found at this Tm N.d. 0.00
mm4__chr19 831.91 Kbp_chr19_831907_F TCGTCGGCAGCGTCGCCTGTGACCTCCCATGG 59.7 0.00
mm4__chr19 831.91 Kbp_chr19_831907_R GTCTCGTGGGCTCGGGCAGAGGAGAGATCGGCTTC 59.9 0.00
mm4__chr19 2.06 Mbp_chr19_2059826_F Primer3: not found at this Tm N.d. 0.00
mm4__chr19 2.06 Mbp_chr19_2059826_R Primer3: not found at this Tm N.d. 0.00
mm4__chr17 75.73 Mbp_chr17_75731007_F TCGTCGGCAGCGTCTCAATGTGAGGGCAGCTCAG 60.0 0.00
mm4__chr17 75.73 Mbp_chr17_75731007_R GTCTCGTGGGCTCGGCCCAGACCCATTTCTCCACC 60.0 0.00
mm4__chr17 42.55 Mbp_chr17_42548160_F Primer3: not found at this Tm N.d. 0.00
mm4__chr17 42.55 Mbp_chr17_42548160_R Primer3: not found at this Tm N.d. 0.00
mm4__chr15 74.37 Mbp_chr15_74369587_F TCGTCGGCAGCGTCACTTGTGGTCCTCTTGGCAG 59.8 0.00
mm4__chr15 74.37 Mbp_chr15_74369587_R GTCTCGTGGGCTCGGGTGACCATGGCCATTGGAGA 60.0 0.00
mm4__chr15 29.57 Mbp_chr15_29570862_F Primer3: not found at this Tm N.d. 0.00
mm4__chr15 29.57 Mbp_chr15_29570862_R Primer3: not found at this Tm N.d. 0.00
mm4__chr13 97.96 Mbp_chr13_97958232_F Primer3: not found at this Tm N.d. 0.00
mm4__chr13 97.96 Mbp_chr13_97958232_R Primer3: not found at this Tm N.d. 0.00
mm4__chr13 40.64 Mbp_chr13_40635789_F TCGTCGGCAGCGTCCAGGAAAGGCCTCTGGATGG 60.1 0.00
mm4__chr13 40.64 Mbp_chr13_40635789_R GTCTCGTGGGCTCGGGGCAGCAGGTAGAGTACAGC 60.1 0.00
mm4__chr12 32.54 Mbp_chr12_32536438_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 32.54 Mbp_chr12_32536438_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 131.81 Mbp_chr12_131808951_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 131.81 Mbp_chr12_131808951_R Primer3: not found at this Tm N.d. 0.00
mm4__chr12 11.82 Mbp_chr12_11820987_F Primer3: not found at this Tm N.d. 0.00
mm4__chr12 11.82 Mbp_chr12_11820987_R Primer3: not found at this Tm N.d. 0.00
mm4__chr11 68.27 Mbp_chr11_68272126_F TCGTCGGCAGCGTCGTTGTCTGCTCTCAGGCCC 60.3 0.00
mm4__chr11 68.27 Mbp_chr11_68272126_R GTCTCGTGGGCTCGGCGCCACAGACCTGCTCAAAA 61.1 0.00
mm4__chr11 44.54 Mbp_chr11_44535698_F Primer3: not found at this Tm N.d. 0.00
mm4__chr11 44.54 Mbp_chr11_44535698_R Primer3: not found at this Tm N.d. 0.00
mm4__chr10 8.58 Mbp_chr10_8584817_F TCGTCGGCAGCGTCGCCAGGGCAATGGGACAAAT 61.2 0.00
mm4__chr10 8.58 Mbp_chr10_8584817_R GTCTCGTGGGCTCGGAGCACGTGAAGGAATGCAGT 60.2 0.00
mm4__chr10 70.48 Mbp_chr10_70478876_F Primer3: not found at this Tm N.d. 0.00
mm4__chr10 70.48 Mbp_chr10_70478876_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 88.53 Mbp_chr1_88529558_F TCGTCGGCAGCGTCACCAGGATCTAACTTTTGGCTCA 59.6 0.00
mm4__chr1 88.53 Mbp_chr1_88529558_R GTCTCGTGGGCTCGGGCAGAAATGTGCTATGGGAGG 59.0 0.00
mm4__chr1 5.66 Mbp_chr1_5662443_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 5.66 Mbp_chr1_5662443_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 3.42 Mbp_chr1_3418850_F TCGTCGGCAGCGTCCGCTGACCCACTCCTAGGAG 61.3 0.00
mm4__chr1 3.42 Mbp_chr1_3418850_R GTCTCGTGGGCTCGGTCAAACATCCTGCTGAGGCC 60.3 0.00
mm4__chr1 229.63 Mbp_chr1_229626476_F TCGTCGGCAGCGTCCTGGTCCAGAGGAGATCCCC 60.7 0.00
mm4__chr1 229.63 Mbp_chr1_229626476_R GTCTCGTGGGCTCGGCAGGAGGGAGTTCGCCTTC 59.7 0.00
mm4__chr1 22.96 Mbp_chr1_22956715_F TCGTCGGCAGCGTCTTTCAGAGCAATCCCTCCGC 60.3 0.00
mm4__chr1 22.96 Mbp_chr1_22956715_R GTCTCGTGGGCTCGGTGAAGTCGACAAGGTTGGGG 59.8 0.00
mm4__chr1 22.29 Mbp_chr1_22285804_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 22.29 Mbp_chr1_22285804_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 205.53 Mbp_chr1_205532682_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 205.53 Mbp_chr1_205532682_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 2.23 Mbp_chr1_2227628_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 2.23 Mbp_chr1_2227628_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 175.73 Mbp_chr1_175726895_F Primer3: not found at this Tm N.d. 0.00
mm4__chr1 175.73 Mbp_chr1_175726895_R Primer3: not found at this Tm N.d. 0.00
mm4__chr1 11.68 Mbp_chr1_11681424_F TCGTCGGCAGCGTCCGAGGGCATTTCTCTCGCTT 60.4 0.00
mm4__chr1 11.68 Mbp_chr1_11681424_R GTCTCGTGGGCTCGGCTCGGGCTAGCTCAGATGTG 59.9 0.00
mm3__chr2 3.31 Mbp_chr2_3307552_F Primer3: not found at this Tm N.d. 0.00
mm3__chr2 3.31 Mbp_chr2_3307552_R Primer3: not found at this Tm N.d. 0.00
mm3__chr2 10.04 Mbp_chr2_10043881_F Primer3: not found at this Tm N.d. 0.00
mm3__chr2 10.04 Mbp_chr2_10043881_R Primer3: not found at this Tm N.d. 0.00
mm3__chr1 2.47 Mbp_chr1_2466592_F TCGTCGGCAGCGTCCATGGCAGGAGGTGGTCAC 60.3 0.00
mm3__chr1 2.47 Mbp_chr1_2466592_R GTCTCGTGGGCTCGGGAGGCTTGTGTGGACACTGA 59.8 0.00

Off-target amplicon sequences

Primers underlined, off-targets in bold.

ontarget_mm0__chr7 5.53 Mbp_chr7_5529702 TGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT
AGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG
mm4__chr20 59.45 Mbp_chr20_59452488 GCGATGCTTCAGAGAGCACTGGGCAGGAGAGCCGGAAGTAGTCAAGGCCGGCCGCTGTGGGGTGGGAGAGAGGGGACAGT
A
mm4__chr9 135.33 Mbp_chr9_135331477 CCCTGAGAGGAGAGAGCAGAGAAAGAGATGGGGGCTCTGGCTGGGGGATGAGGCCCCACGGAAGCTGCCAGGGTGTGGCA
GAAATGGCCAGGGCGGGGCAGGAGCTGAGGCTGGAGTTCATGGCCTTGCACTG
mm4__chr19 48.63 Mbp_chr19_48629095 TCGTTCTGTGTCTGACCTGCCTCTTCCCCTGCCCCAGCCTGCCCTGGCCTCTCCTGGCTCGCCAGAACCCCTGCCCATCC
TGTCCCTCAACGGTG
mm2__chr11 120.34 Mbp_chr11_120336285 CTTCCCGAAGTCAGAGGGGAGGGGGCCATAAAGTCGGCGGGGGACGGGAAGCGCGAGCTCGCGCCGGACGCGGCCAGGGC
CGGGAAACCAGAACCGGAACCGG
mm3__chr20 50.04 Mbp_chr20_50040349 GGGCCTGAGACTCCGTAAACAGCCCTGGCTCGTAAAGCTGATCAAAGCAGCAGGCCTGTCCCAGCCCTGGCCACTTCCTG
CAGGGCCCAGAGCCCTGGCCTGTGGGAGATCCTGGGAAGG
mm4__chr14 75.47 Mbp_chr14_75470352 AGTTACGGCCGTTGTGTGTAACTCCTAAGTACTGTAGTCTCTGGGTGTCGGGGGTGGCCAGGGCGGGGGCGGGGTGCATT
TCCATCCTT
mm4__chr9 134.94 Mbp_chr9_134938233 ACACTCTCCTCCCGGTGTTGACAGTGACGGATGTGGTCAGGGCAGGACCCAGACAGGGAAGAGCTGAGGTTCCTGGTGGG
TCAGTTTGGCTCTTCCCTGCTCTG
mm4__chr22 47.78 Mbp_chr22_47784141 ATGAACATGGCAGAGGCAGGCAGCACCCCTCAGAGCGCTGAGCTGTCGGGAAGCCCCCGGCCCTGGTCACTCCCCACGCC
TGCCCAGAGAGGCCGCGCCAGATCCATTAAAAGTGTTTCAGCAGCTTCAGAGGC
mm4__chr3 98.52 Mbp_chr3_98522620 CCACCTCCTGGGCCTCACGGCGGGGCCGGAGAAGGCCAGGGCTGGGGCGGGGCAGCCGTGCTCGGCCCCGGGCCAGGGTC
CCCCGGTACCCGACCAGGCCCCGCCCTAGAGGGCTCGGCCCTGGGACAAATC
mm4__chr8 144.42 Mbp_chr8_144416549 GGTACTGCCACTAGCCTCCTCCGCCTCCTGGAGTTTGCCCAGGGCCCTTTGCTGCCGGCTGGCAGGGCGGAGCTGGCGGG
AAGAGGCCAGGGCTGGGGGACCCGTCGGGTGGGGCTGTTACCTTTCCA
mm3__chr3 168.10 Mbp_chr3_168095660 CCAGATGCCTCCTGCCTTTTTTCCTTCTTCCCACTTTTTGGCCCCGCTGCCCCCGGGCAGTTCTTGGAGTCCCGCCCTGG
CCACTCCCCGCCCTATCGCGGCGGCTCCCACTGCACGACTTTGTTG
mm4__chr5 2.27 Mbp_chr5_2271664 GGAAGGTGGTGAGAGGAGGAAGGTGGTGAGGGGAGGGAGGTGGTCAGGAGACGGAGGTGGTCAGGGCAGGGGAGTGGTGA
GGGGAGGGAG
mm4__chr14 104.28 Mbp_chr14_104278638 CCTGGTGGCTTGGGTAATGTGGCAGCCCCCTCCCCAGGAGTCCCTGCACTGGCCACTCCAGGCACCAGGATTCTTTTTGC
CTTAATGAATTCCCCTCAAGTCTCCGGT
mm4__chr6 170.46 Mbp_chr6_170462184 CTCCAGAAAGTCCCAGGCTGGCTAGGGCCGGCTGCTTGGTGACACAGGCTCCTGGGTCCTGGGACCAGCCCTGACCACTG
CTGGCACAGCAAAGCTGGTCACCCGACGGCTGCCCTGTGGAGAGGACCAGGCAGACAAA
mm4__chr14 99.56 Mbp_chr14_99563437 GGAGACCTGGGAAACTGGCCCAAGCCACGCCCCCCTCACCCTGCCCTGGCCACCTTCTGCTCCTGAGCCCACAGGCACAG
GCAGCTTCGAGCACAACTGGGCCATCTGGGAAT
mm4__chr17 77.36 Mbp_chr17_77364600 AGAGCGGAGAGGTTCCTCTTCTCTCTTCTCCATCATCCTGTGGAGGTGTCAACATGTAGCCAGAGATGGTCAGTGGCAGT
GAGGAGAGGGGGGCAGGAAGAGACCAGGGCTGGGGGCTTGCCAGCCCACAGTTCTA
mm4__chr15 75.29 Mbp_chr15_75294385 AGTGAGCCTGGACAACAACGTGGAGCCTGCACCAGGAGTGGCCAGGGAGGGTTCTCCCCATAACAACCCCACTGTACAGC
AGATCGTGCAGCTGTCTCCTGTCAT
mm4__chr7 149.28 Mbp_chr7_149282074 CTTCTTCCAGCAGCGCAAGAGCCTGCTGCTGCACCAGCGCCTGCACACCGGCAATGGCCAGGGCTGGCCCGCCTGCCCCT
ACTGCGGCAAGGCCTTCCGCCGGCCCTCGGACCTCTTCCGGCACCAG
mm3__chr7 150.38 Mbp_chr7_150379305 CAATGGAGTGGCTGGGCGGCCCCGGGGATGGCCAGGCCCGCCAGCCAATCAGGCACCCGTGCCCGCCCCGCCTGCCCCGG
GAATGGCCAGGGCCGGGAGCCCGGTGCCCAAGTCGCCCTCGGGGTGGCAGTTCCCGTTAA
mm4__chr17 3.60 Mbp_chr17_3600763 CAGAGCCCTTGGTGCTAACAGTATCACCGGAGTCTACAGGGCACAGTGCCTGCTCAGAGGCCTGCAGGGGAAGTCCCGGC
CCTCCTCCTGCCCTGGCCACTCCTGCCGGGACTCCCTGGCTGCTCTCCTCTTCTTGCCG
mm4__chr5 179.82 Mbp_chr5_179821852 GGTTAGGGAGGTGGTTGTGGCCAGGAGGGCGGGAGGTGGCCAAGGCCGGCCCCTGGGAGGGTGCAGTGCTAGGACCTCCC
TCTGGAGCGCTGCCAGCATACCAGGCCCTCTCCTA
mm3__chr15 27.59 Mbp_chr15_27591258 AACAGCTACTGTGCACTGGGCTGCCCTGGCCTGGCCACTTCCAGCACCCCACTGCACATGGTGAATCCCCAGCATGTC
mm4__chr10 126.80 Mbp_chr10_126797175 TGAGCATCATGGAGAGCACCGTCCAGCTCAGAGGGGCCTCGGGAGAGCTGGGAGAGGCCAGGGCTGGGACTCAAGTCCGC
ACTGGCATCCACCCCAGACAGACTCAGAACCCTCACCAGGTACACAGGTGGACTCAGC
mm3__chr20 37.41 Mbp_chr20_37406613 GTGGATGTGGGTGGGAACAGCATGTGTGCCCGTGGACCCGTGTGAGCTTGGGCTCGCACCCCCAGCGCTGGCCACTCCCG
GCGGGACCCACGTGTGCCCGGGGGCGCAGGGCCACGTGCGAGCGCAACCGGGACAAAG
mm4__chr17 43.90 Mbp_chr17_43900231 GGCTTGGCATTGGTCAGAGACCTGCGAGGCCCTCAGTGGGCTCTGAAAGAGGCCAGGGCAGGGACCCTTCCCTTCCTGCC
AGGGTATGCCAGATTCCC
mm4__chr19 7.77 Mbp_chr19_7765326 AGGTTCTGAGGGTCTGGGGTCCCCAGGCCTGGCCTTTTGGCTATGAACAGAGCCTGGAGTGGCCAGGTCTGGGAGGGAGG
TGGGACTGAGAGCCAAGATGTTGTCCCTGG
mm4__chr15 31.81 Mbp_chr15_31813009 ATGGATTCTCAAGGCCAGGCCCTTCCCTGGCCACTTTCGGAACCCAACCCAAAACACAGAGAAGGTAGAAGCAAGACACC
TGATGCG
mm3__chr2 69.99 Mbp_chr2_69989558 CTGAGCCCTTGGACCTGCCCAGCCAGCCCTGCCAGTCCTGGCCAGTCCCGGCGCTCATTTGGGGCGTGGAGGGGGCACTA
AGTGCACAGCCGCCCCCAGAGTAAACAGCCTGATGAGCACGGGAAAACAAGATGC
mm4__chr4 1.80 Mbp_chr4_1800075 TGTTGCTGATGGGGAGACTGAGGCACAGGGCCCTGGGGGTTCCAGGAGCAGGAGGAGGCCAGGGCTGGCCTGTGGGGCTC
TGGTGTTGGCTATAGGTGAGGTGGACCCCG
mm4__chr4 3.24 Mbp_chr4_3242546 CTTTCCTTAGACCCGCCAGGTCAAGTTAGCCGCGTGACGGACATCCAGGCGTGGGACGTGGTCAGGGCAGGGCTCATTCA
TTGCCCACTAGGATCCCACTGGCGAAGATGGTCTCCATATCAGCTCTCTGCAGAAGGGAG
mm4__chr20 32.99 Mbp_chr20_32985583 ATCCCCTACCCTTGAAGGCTGAAATTGGACCCCAGCGCTGGCCACTTCCAGCCTTAACCAAGCTGCATTCAGCCCCTCCA
GCCTGCAAGTGTTGTGCAGAC
mm4__chr14 53.36 Mbp_chr14_53355727 GTGTGAAGGAAACCACAGCAGATGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNCTTAGCACAGATACACTAATTTCCACGTCCAGACTGGT
mm4__chr11 131.46 Mbp_chr11_131463880 AGGAAGGAGAGAGGATGGGGCCTGGAGGCGGCAGCCTGCCCTGGCAGCTTCTGGCTCCCAGAGATTGGTTTATCACCAGG
TTTCTGATGAATGTGTGAGAAACGGGACTGG
mm4__chr19 9.85 Mbp_chr19_9847288 CACTTGGCTTCCGAAACGTGAGTCACTCAGATGCTACTCTGCTGCCGTCTTGCCCCCTCTGCTGGCAGCCCTGTTCCTCC
TAACTCCACCAGCCCTGGCCTCCTCCGCCTCCTGGAGAGTCAGAGCCGATG
mm4__chr3 194.27 Mbp_chr3_194268138 ACAGGCCCCTTTCTTCTCACGCTCAGCTGCACGAGGCCCCACCAGGCCTGGCCTCCTCCGACGCTATGACGCAGTGTCGG
TGCCTCTCACGTCCATGGGCCAGACCGTGTGACTCAGCCTCTCAG
mm4__chr17 30.70 Mbp_chr17_30697310 CCCTGCCTCAGTTTACCTGTCACTTCCCCAGGGCTGGGAAGCCTTCTTTGTGCCGGGACTGGCCNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNCTCAATTCATTACACAATGAATCCTGGCCCTGA
mm4__chr15 77.03 Mbp_chr15_77034247 CAGCACAATGGCTCAGCAAGCCCTGCACCCCTTTCCCCCTGCCCTGGCCGCTTCCTCCACCTGTCCCCAGCCCCTGCTGG
CTTGCACCCTGTCCTTTGCT
mm4__chr11 76.65 Mbp_chr11_76653181 AGGGCAGTCAAGTCACCAAGTCGCAGGGAGGGGCCAGGGCTGGGGGGCTGCTTTCAGTGCATGAGAAAGGACCTCAGGGT
CCAGAGTTCAGGCCCCATTCTCC
mm4__chr13 113.08 Mbp_chr13_113076882 CATGGAGCTGCCCTTTCCCGAGGCCGGGGCCCCGTCCCGTGTGGGGGCTGCATCCCCCTCTCCGTCCGTAGTGGCCAGGG
CTGGACACAGCTGCGCTGCGCTGACAGACCCCGCATGGAGAACATTT
mm4__chr1 155.01 Mbp_chr1_155005757 TGGGGACAGGCAGATGGATAGGACCCTGGCATGGGGTGTGCCCGCCCTGGCCTCTTCCCCCTCCTTGTGCTGAGTAAACT
CGTGAGTAAACTTGACGTTTTGCCCGGACAGTTTGAAGT
mm4__chr10 68.83 Mbp_chr10_68827191 TAACTTGGAACGGCCCCAAAGCCAGGTCGAAGGCGAGTCTCCCCGCCCTGGGGACTCCTGGCGCTGCAGTTCGCACGGCG
GATGGACGCAGTCAGGTTCTCCAGGGAGCTGCGAGGAGCCCCAGGACTTGGAAAA
mm4__chr1 201.52 Mbp_chr1_201516894 ACAGAGAGGCCATACCATGAATTCTTAGTCATACCTGACCTGGCCACTCCAGGAGCGCCAGAAGAGTTGCTGGAAGCCCA
CATAGACA
mm4__chr19 18.76 Mbp_chr19_18761188 GAGCTAGTCCCCTTGGCAAGGCCGTCCCGGCCCTGGCCTCTACTGCCGCGTTGCTTTCCTGCACCCCTGCCCTGGCCCAC
GTTGCTGCTCCGTCCTTAGCTGTTG
mm4__chr18 79.44 Mbp_chr18_79444532 CTCGCTGCTCTGGAGGGAGACCCCAGGCCCCTCAGGCCCTTTCCCGCATCTGAAGGGGCCAGGGCAGGGCGTCCGGCAGA
TGAAGCTGCCCCTTAAAGCCAGGTCTGG
mm4__chr16 75.25 Mbp_chr16_75250781 CGACCCTGCTCTAGCTCTTGCGGGTCCCCCAACCATGGACTCCAAAGCCTTCATGTCCACACTGCAGAACCCGGCCCTGG
CCCCCTCCCGCGACACTCGCGTGCGGCTAGGACTCCTGTGGCCAG
mm4__chr14 100.50 Mbp_chr14_100503684 CCCCGGCTTCACTTACACATCACAACCTTGTCCCCAGCCCTGGCCACCTCAGCCCCCGTCCCCGAAGACTGCTGGATTCC
CGTTG
mm4__chr15 41.54 Mbp_chr15_41536572 GATCATGCCTCCTCAGCCTCTCTTCTGGGTCCTCATCCTCAGGCAGGCAGTGGCCAGGGCTGGGCCTGGCTCTCTTTGGA
GGAGAAGGGACCAAAGC
mm4__chr15 60.91 Mbp_chr15_60906678 GCCAGACTGTATCAGAGGCAAACAAGTGATTTAAGGAATTTTAGGAAGCCAATCTAGTACCCAGCCCTGGCCAATCCAGG
GGCTAGCTATGCAGAAATCTTACTGATAGCTTGGGATGGATACTACGGCCACG
mm4__chr12 6.38 Mbp_chr12_6384200 CACTCCCACTTCCTGAGCTCCGCCATGGGAGCCCTGGAGGCCCGGCCTGGCCGCTCCCGGCCCTGGGGTGCACATCGGCC
CTGAGTCC
mm4__chr13 113.31 Mbp_chr13_113313477 GATCCTGGACGTTGGTTCCTTTTTTAATGGAGAACACGCATGTGATTCCAAATGGAAAAAGGCCCGACAGCCCAGCCCTG
GCCACTTGCCCCTCCTGGAGGCAGCCAGTGTGGAGATGCCAGTGT
mm4__chr6 31.93 Mbp_chr6_31933901 CTGACTCCTGTGTGGCTCTCCCCACAGAACCCTACTCTTATGACTTCCCTGAGGACGTGGCCCCTGCCCTGGGCACTTCC
TTCTCCCACATGCTTGGGGCCACCAATCCCACCCAGAAGACAAAGGG
mm4__chrX 38.80 Mbp_chrX_38804040 GTCCAGCTGCTCCAATCCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGCCCGGGT
ATCAGGCGAGAGGCGGAG
mm3__chr11 44.57 Mbp_chr11_44565349 GGAGGACGAGGTAGGCGGGAGCTGGGCGGGACCGTTAGGCAGCGCCGTGAGTGGACAGGGCTGGAGCGGGGCGGGCTTAA
TCCCCAGTGTAACCTGGGGGCGGGGCTGCCGGGATAGAGGAGAGA
mm4__chr11 57.44 Mbp_chr11_57440081 CGAGAGCCCTGGCACTAAATGAGAGCTACTCACTGAGAACAGCTTGGTGCTGAGACCCTGGGGCATGCAGAGGGCCAGGA
AGTGGCCAAGGCAGGGCAGAGCCACTTGTTTTCCC
mm4__chr4 76.21 Mbp_chr4_76214021 GGTGGCCCTGAGTGGCTGCGGCGCCTGAGCCAGGCCCGGCCTGGCCGCTCCCAGCGCCCTGCACCGCAGGCCCAGCGCTC
GCCCCACCGAAATTGCCGAACCTG
mm4__chr7 1.90 Mbp_chr7_1898444 GCACTCTCCACCTGCTTCTTCAGCTCTGCGGGAGGGACGGAAGGAGACAGTGAGTGCGGCACCAGGCCGGAGGGGTGGGG
ACCCGGGAGCGGCCAGGGCAGGGAGGAAGCCGAGTACAGGTG
mm4__chr22 50.27 Mbp_chr22_50266457 GGCCAGCCCAAAGTAGCATAGCATGGGGGGCAGAGGAGGCAAGACCCGCCAGAAGGGGCCAGCGCTGGTCCCTACCCTAC
AGGAGGGGCCCTGTTTGACCCTGCTCCCCAACCTGGGCCAAGGATACAGTC
mm4__chr2 170.82 Mbp_chr2_170820044 AGCATATGAACCAGGCACCGGCGTTGGAGCTGGCCAGGGCAGGGCGGGAGGAACGCGAGGCCTGCGCGCCTTTGTGTGGT
GCTTTCGCCTTGATGGATTTCTTTTCTGACGCTTTCCACATTTCCCGCTCCCTTTTGC
mm4__chr12 125.28 Mbp_chr12_125281222 TATGCAAATGCGGAGTGGGTGTGAGCAGGAATGGGCCAGGGCAGGCAGGGAAGCCCGGGGACTGGGGCGGGCGATTGAGG
AACCTGTGT
mm4__chr14 61.28 Mbp_chr14_61280889 GGCAGCGAGAAGTACCAGGCGCACGAGCAGGGGGGCGGCAGCGCCCGGCCCTGGCCAGCCGCGGCGCACACCGAGCAGTT
ACCGGTGCCCGGGTCGCCTGTATAGTCGTACTCCAGCTCCTTGATGCCGTTGGAGGAGT
mm4__chr11 62.04 Mbp_chr11_62042408 GTAAAGGCAGGTGGGGTGAGTTAGAGTGGTTGCAGTAATAGTGGCACACACGCTGTGCCACGGGGAGTGAGCTGGAGGTG
CCCAGGGCTGGGGCCGCCCTATAATGCCAGCTGGA
mm4__chr12 132.20 Mbp_chr12_132202715 ATGAGCCCTCCCAGAACTCAGGGACCCAGTGCCTGCAGTGGCCAGGGAAGGAAGGGGGTGGTTCCAGAGACGCTGGGGTG
TGGCGGGATGGGTCGCGGCCACCCGTCTCAGTACCTGCCTTTCTTAGGGTTGTGGG
mm4__chr2 219.04 Mbp_chr2_219041421 CTCTCTGAAGGGCATGGAGGGGACCTCTGGCCACGATTTCCCGAAGGATAGGGTCTCCCGGGACAGATACCTTCCCTGGC
CACTCGCGCCGCTCCCTGAGACCCTGGGACCTTGGGACTAACGCTC
mm4__chr1 43.37 Mbp_chr1_43368199 TTCCCACCCCTTCCCTCGCCGCCCGGGCCCGCCCTGGCCAGTTGCAGCGGCCTGGCCACCTCAGGAGCACGCTGTCTCTT
C
mm4__chr17 2.95 Mbp_chr17_2947448 ATAGCAGTAACACTGGCCCGTCCAGCCCTGACTCTAGCCATCCCAACAAGCCCGCCCTGGCCACTCCCAGGCCAAGACTC
AGCCCTCACCGCTTGCTTCGTT
mm4__chr17 46.02 Mbp_chr17_46021005 CCACCAGGCCACTCAAGTAGCAGCTGAGAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGT
TCTGAACAGATGGGCACCC
mm4__chr3 48.66 Mbp_chr3_48664006 CCTCCAAAGGCGGAATCGATAACGAGCTGCAGCGCCGGGTGCAGAGGACGCGGGCATCCCGAAGCCCAGGAAGAGGTCAG
GGCCGGGACCCCAGAACGCTCCACAGGGTGCGGCTCCCGCGATGGGGTGGATCCTGGTTC
mm4__chr16 55.64 Mbp_chr16_55636355 TCATCAGGTCAGTGCTGGTGCAAAGAGATGGAAGTGTCCAGGGCAGGGATTACAAACTCTCAAACATCACAGAGGCTGGT
CAGAAACATAACGCAGGTAGGAAGTAAGGAGCCTGAGGC
mm4__chr8 28.70 Mbp_chr8_28701459 GACGCGGTCGGGAAAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACGCCGGCTTCGGCCATTTCCGGCGC
GGCGCCCTTCGGCAAGTTCCGCAGTCGCCTGTCGGAAATGG
mm4__chr2 5.35 Mbp_chr2_5348964 GAGCATGAGCAGGTGTGGAGGGGAGAACCATCACCTGGACATGGGGGAGGGGCTCAGTCAGCACCAGCCCTGGACACTTC
CAATGCAGGGGCCATGAGCGGATGCTGGTGCCCAGAAGCCAAGCAGCACGAG
mm4__chr9 135.61 Mbp_chr9_135612567 GATGGAGGCCAGAAGCTGTCGCGCTGCGCTGAGCCCTGCCCAGCCATGGCCACTTCTGGAACCTGCATGCGGAGCTGAGC
CAGTGCAGGGTGGGGCCTCCCCAGGGCCCCAGGGGGTGTAGGCAGGATGCAGGATG
mm4__chr1 93.60 Mbp_chr1_93599598 CTGGCTGCAGTGAGAGCAATGGGCTGGATGGGGCCAGGGCTGGAGGCACAGGTCAGAGTCTTT
mm4__chr4 152.09 Mbp_chr4_152090667 CACAAGCACATTGGCGGTTGCCAATTGCTCCCAGGCCTGGTCACTGCCTGCGCTAACTAGCTCTCTCTAACTGTGTAAGA
GGCTTTGAGTGTAAAATGTACAGGCAACAGAAAAGATAGGCCTGGGGTGAGT
mm4__chr8 100.89 Mbp_chr8_100888452 GTGGGCTTACCTGCTGATGTTCTTTCTTTATGTGTGAATCCCCAGGAATTGTGAATGGTTCCTCAAGTCTCCATGCAAAC
AAGCCAGCCCTGGGCACTTCCCAAGCAACACCCATTCTCCACTCTCATCTGCTCGGTC
mm4__chr1 56.53 Mbp_chr1_56525642 ACCCTGCTGCCTTTGTCTTAACTCCACAGCCCAGCCATGGCCACTTCCTGCTTGTAACCACAGCCCAGAGACAGTGATGG
mm4__chr2 241.95 Mbp_chr2_241953837 CAAAGGCCTGGAGCAGATGTACCCAGGCCCCAGGGAGGAGCCGGAGGTGCCCAGGCTGTGGGGAGGAGCCGGAGGTGCCC
AGGCCGGGGGGAGGAGCCAGATGTGCCCAGGCCGGGGCTGTCCTGAGGTCTCCGCT
mm4__chr6 155.64 Mbp_chr6_155636783 ATGCTGTGCTGCTCATTCCTGGCTCCCGAAGAGGACAGGGCAGGGTTCACATAGATCTGGAGAAGGAAATGCTGACCCCT
GCCACCCCCCTGCCGCCCACACCCTGACACTTCACA
mm4__chr1 11.52 Mbp_chr1_11522393 GCGTGATAATCCACTGCAGCTCCGAGAGGCACCAGAAGAGCCCCTCACACTTCGGAGCCAGCCCTGGCCACGTCCCCAGC
TCCTCTACCCCACTGCCAGGATGTGGCATTGCTCCCCATCATCACCG
mm4__chr12 52.06 Mbp_chr12_52061120 GACAGACGTGAGGACAGGACAGGCACTGGGGAAGGCAAGAGGGCTGCTCAGATGCTCAGGGAGGGGCCAGGGCTGGGAGT
GGAGTGGGGCCGAGGGAGCATCTCTATATCTATGGATGAGGCTGGCGGGTATTAGGGTTC
mm4__chr16 74.71 Mbp_chr16_74714135 TAATGGGGTCTGAATGGCGGGTGGGGGTCGGGAAGGGGCCAGGGCCGGGCTGAGGGCAGGACGGAGGGGGTGGGAGTTGT
CACTGCGTCTTCAGGTGGGGTTTCTCTGGAGTG
mm4__chr16 2.96 Mbp_chr16_2956399 TGCAGACATTTTGGAGGCCAAGGCCGCTAGCGCCCCAGCAGCCATTCCGAGCCGACTGGCCTGGGGCCCAAATGAAGCCA
GCCAGCCCTGGGCACTCCCGGGCCCTGCCAGCCTTGGGGTCCTCATCTGAGAAGGGCG
mm3__chr19 518.94 Kbp_chr19_518942 CGCCTTCAACAACAACGTGAGCGTGGCCTACGAGTGCCTGAGCGCCGGCGGGCGCAGGAAGAGGCCGGGGCTGGACGGGC
GCACCTACAGCGAGCTGC
mm4__chr17 28.52 Mbp_chr17_28524635 GAGCAAGTCCAGGGCCACTGCCCAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGACAAGTATCCTGGCTT
TGGCTTTGAGG
mm4__chr1 1.18 Mbp_chr1_1182573 TGAGGGCTGGACCAAGGCGGGGGCTGATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACTGAGGAGTTGGAAAGGGG
mm4__chr9 136.52 Mbp_chr9_136517726 AGCAACCCACAGATGTTCCCGGGGCTGCCCCTCCAAGGCTGCCCAGCCTCGACTCGGTTTCCCGCCCTGGCCCCGGCCGA
CGCACCGGGCATGCAGATGCACTGGAACTCC
mm4__chr11 131.25 Mbp_chr11_131254449 GAGAGAAGAGTCGCTGAGCCTGAATGTGCTGGTGCAACACCTGGGGTCTTGTCGCCTGAAGTTTCCAGGGCAGGAGGCCT
GGGAGGAGCCTGAGGTTCTGATCCCAG
mm4__chr7 157.43 Mbp_chr7_157428886 CCTCTGGAGGGACCTGGATTGCCGGGGCCTGAAGTGGCCACAGCTGGGGCGGGGGGCCAGTTTCCCTGGGTGCTCTCTGC
ACCTGGGAGGGACATCCCAGGACATCCCCCAGTCTCACTGGGAAATCTGCCCAC
mm4__chr19 2.23 Mbp_chr19_2229414 CAAGGAGAGGAGGACTGGGAGCCAGGAGCCAAGGCGGAGGCTGTGGCCAGGGCTGGTCAGCCTGGCGGGTCAATGCGGGC
ACCTGCGGGCTGGACAAGCTATGCTGGGTCTCTTAGGAGATGAGCTGCAGGTAGGGTG
mm4__chr1 1.44 Mbp_chr1_1440125 CGTCAGACCCAGGACTGTTCAGGGAGGAGCCCCGGTCAGACTCCCACGTGTGAAGACCGGGCCCCAAGTGGCAAGGGCTG
GCCTGGGGCGGGCAGCTTGGGTCCTGGACGTTGATAGGAAGCGGAAGGGGA
mm3__chr8 139.98 Mbp_chr8_139982937 TTCACACTTACCACCCCACCACACTCCCCTCCACCATGCTGCTCCCCCTGCCTGCACGGCTCTCCCTGGCACACTGGCCC
TGGCCACCTCCTGCTCACTCGTCACGTCGTGCTGTAAA
mm4__chrX 107.98 Mbp_chrX_107981494 CTCACCTGCTCCTTCTGTCCAATCCCCTCCGGCAGTGGCCAGGCCTGGAAACACTGTAGCCTGGCCCTGTCCCTGGGGAG
GTGCCAGCTCCTGGAGAGAAGTAGGCAGAGCTGAAACACATTCTGGGGACAGGG
mm4__chrX 105.22 Mbp_chrX_105219693 GCTGCTCCTTCTGTGCAAACCCCTCCGGCAGTGGCCAGGCCTGGCCACCCTGCCCCCTGCTCCCACTCATGGGGAGACAC
CAG
mm3__chr7 30.85 Mbp_chr7_30854026 CTGTACATCCACTCCTGGCCATGAGCTGGGCGGGACGTGGCCAGGGCCAGACAGCAGCAGATCACAGGGCTCCGGGAAGT
CATGGCAGAGCTGT
mm4__chr5 177.13 Mbp_chr5_177133778 GGGTGTGAGGAGGAGGAGGCGGCGGCGGAATAGGCCGGGGCAGGTCGCGCTCGCTGCCTTCTCCCCTGAAGAGA
mm4__chr7 47.70 Mbp_chr7_47698361 GCAGTGTGGTGGAGGAAACAAGCCCAGGATGTGCCCAGGGCAGGCTGGGACCTGGGAGGTGGAGGTGTTGACCTGGCTAG
CAGAGCCCTGGAGGAACAGAAGAGTGGGAAGACAGAAGGGC
mm4__chr10 691.18 Kbp_chr10_691185 ACCCCAGGTCCAGAACAATCTCTCTCCTTTCTCTTCCTGCACCGGCCACTTCCCGCCCTCATCACGTTTGACCTTTTTTA
TCTTCTCTGTATTCTTTGTGAGTGTAAGCGCCTGG
mm4__chr5 173.79 Mbp_chr5_173785985 TCACCTTCCTCCCCTTTCAGAAGATAAAATCCAAACTACTCAGATAGGCAAACGGTCATTCCTGCCCTGGACACTACCTG
CTCCACCCACCTTGTACCAAGCTCCATGTCTACACCCTCTGCTGGG
mm3__chr11 66.90 Mbp_chr11_66902672 CAACCTCAGCCACTCTCCTCCACCATCACTCTTGCCCTGGCCACTCCCGGGCCTGTGATCACACCCTCCTCTTCCTCCTC
CAGCTGTATCACATCACAGTCACACCTGTGTGTCCCCCAAACTCTGACCACAGCCCT
mm4__chr1 16.14 Mbp_chr1_16139301 CCCTACAGGCAGGAAGCTGCCCAGACACAGGGCTCACGGTGCAGGGCTGTCCAGGGCTTGGGCAGCTGCTGAAGTGGCCA
GGGTGGGTAGAGGTCACCCTAGGGAGCTCCTCCAACAACCCTAC
mm2__chr3 149.75 Mbp_chr3_149752480 TGTCCTACGATTTCACCCGGCAGCGCCGACCCCGCCTTCGCCACTTCCGGCGCCCGTGTCCCGGCGCCTAGAGGCTCCGG
CGTCGCAGCGTCCGGAGCTTTGGGTTCCGGCCTACGAACTAACGCGTTACACTCGGACG
mm4__chr16 11.49 Mbp_chr16_11494715 CGTCCAGAGAAGCACAGAGGTTGTGCACCAGGGCTGAGATGCCCAGGAAGGCGCCAGGGCTGGCCCTCGGGGTCTGGAGC
AGTGGCTGGGGACAGAAAT
mm3__chr17 74.92 Mbp_chr17_74923074 CGGTGGTACTGGTCGTTCATGGCGCCCGAAGTGGACGGGGCGGGCGGGGGACACGGAGAAAGGCCCCCCGCAGGGGAGGG
CGGCGGTATTAGGGCTGAGGCATGAGGTTGGAGG
mm4__chr20 38.16 Mbp_chr20_38159011 AGAGATCTGGGCTCTTCCGTGGCTGCCTACAGGGGCTGCGGAAGTGACCGGGGCAGGTTGGTTATGGTTTGAGATAAGTT
GGTGGAGACTGGAGGACA
mm4__chr14 72.81 Mbp_chr14_72809324 CATCATCGTCACACAGGGCTTGGGCTGCCTGGCCCTGGCCACTGCCTGCCAACACCAGGCCTGGTCCCTCCATCATCTAG
GACCTTGGGGAACATGGCA
mm4__chr10 43.26 Mbp_chr10_43263687 GCTCACAAGAGACAAGCAGGTCTCGGAGCCCCTGCTGGCTGCCCTGGGCAGCACAGGCCGGCAGCTCAGCCCCTACCCCA
GCCCGGGCAGTGCCCAGGGCAGGGCGGGCAACCACAGGTGGGGAGAAGCTTCCCAGAAC
mm4__chr11 69.88 Mbp_chr11_69879886 CAGGCCTCTTTGGAGAGCAAGGAGGCTGGGCTGCCCCAGGCCTGGCCCCTTCCTGGGCACAGAGATGAGCATCTGCCATG
CCAGCCCACGTCACTTTCCTGGAGGGCAGGCCTTTGAGAAA
mm3__chr12 54.47 Mbp_chr12_54473594 CACAGTCACCTTCCTCCCACGCAAGCCCCAGCAACGGCCACTTCCGGCGCCTAACGTTGGGAGGTGGGGCGAGGGGCCGG
CACTTCCTGTGACTGGGAGTCTCGGTTCTGGCTGAG
mm4__chr1 8.22 Mbp_chr1_8217374 GTCAGCCAGGCCAGCTTGTGGGGGAGCGGCCGCCCGCGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNGGCTTCTCTCCCCGCAGC
mm4__chr19 53.25 Mbp_chr19_53254906 AAGACGGAGACTCACCCGAGAGCGCCAGTAGCCCCGCGAGATCCGCTTCCGGGTCGGCAGGAACCTGCGCGTACGCGAGT
GCACTGGGGCGGCGCAAGGGGCAAGGGCAGGGGCAGGGCGCGGACAACTGTGGGGATGGG
mm4__chr2 19.57 Mbp_chr2_19568880 CACAACAAACTCCAGTCTGATGTTATAAGTTCTGGGCTACATTCTAGATGTAGCAGAAGGTCAGCCCTGGCCAATTCCAG
CATTTCTTGATCAGGATCCATCTTTTGATCAAGGGCCATTGAGGGTTG
mm4__chr20 3.89 Mbp_chr20_3889778 GTAAGTGTTCCGTGGGGCGCCCTCCCGGCCCGCCCTGCCCCCCCTTCCGGCCCACCCTGTCCCCTTCCGGCCCCGCCGCC
GTTTTTCCCGCGCGCGGACACTGTCCCCGCACGGTGGTCCCTCGTTGGTG
mm4__chr15 101.09 Mbp_chr15_101086434 CAGCTACGTCACCTCCCACGGCCTCCTCCGGCGCCTGCGCGGCTCTTCCCCAGCTCCCCCTGCAGCTACGTCACCTACCC
CGGCCTCCTCCGGCGCCTGCGCGGCTCTTCCCCAGCTCCCCCTGCAGCTATGTCACC
mm4__chr11_KI270721v1_random 8.78 Kbp_chr11_KI270721v1_random_8783 GCACTTTCTCTCTGGGCCAAGATGTCCACAGCCTGCCCCGGCCACCTCCTGGGCCTCCCCACTCTGCCCCTGGGGCTGGC
CTTACTGGGTGGGGATGGGGTTGTGTTCCGGTGTAAATGGTCCCTGGAGGCC
mm3__chr9 125.52 Mbp_chr9_125515672 TGAACCGATCACCAGAGATCAACTAAGTTCGGCCCTGGCCACTTCAGGTTCAAATGTTTGGGAATGCAATTTTGCCCCCA
TTAACTTTCAATAGCAATGAAGAACAGACTGGTCAAGGGGCCAGTCGGTGCAATAAAC
mm2__chr16 28.21 Mbp_chr16_28211954 CCGACCAACAGCCCCAACGCGCCGGAAGTGGCGAGCGCCGGGCGGCCCCACAGCGCGGCCGCCGGGCTGCGGCGCTGGAA
CCGGACCAATCC
mm3__chr11 2.41 Mbp_chr11_2409230 TGAGTGTCTGTGTGTGCACTGGCAGTCAGTGAGCGCCAGCCCTCCCCACGCTCCCACCCTAGGCCTGCCCCTCCTCTGTC
CGCCCTGGCCACCTCCAGCCCATGAATCTGTTCCTGGGCAGACTGATG
mm3__chr20 40.33 Mbp_chr20_40326990 ACGGAGTCCAGTTACAAGGCTTCTCACCCATGACAGCAGGGCAGGGAGTGGCCAGGGCTGAAATAAAATAGTGACTAACT
TCTGAATAAACCACCGGGGTGCCTGGATTGGGACCTCCACCCCATCCTAT
mm3__chr1 227.79 Mbp_chr1_227788017 CCTCCCGGCTAGTTCCTGAGCGCGCTCGAGAGGAGCGTGCCCCGCAGGTGCAGGCGCCGGGACTAGCCAGGGCAGAGCTA
GCAGTTCGCCGCGGGCGAGGGAGGGGCTGTGGGTGTGG
mm4__chr11_KI270721v1_random 5.18 Kbp_chr11_KI270721v1_random_5180 GAGTTTCTCCTGTGCCCCTCCTCCCCCCAGCTCTGAGGCCCAGATAGAGCCAGGGTGTGCCNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNTAGGACGGTGCTGCCTGTCCACCCAGCTGAACGTGGGAGAAT
mm4__chr11 1.95 Mbp_chr11_1949867 GAGTTTCTCCTGTGCCCCTCCTCCCCCCAGCTCTGAGGCCCAGATAGAGCCAGGGTGTGCCNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNTAGGACGGTGCTGCCTGTCCACCCAGCTGAACGTGGGAGAAT
mm4__chr3 51.39 Mbp_chr3_51392901 GGTTTTCCCTTGGGTGGACCAGACCGCAGGCTCCGCGTGGATTTTGCCAAAGCAGAGGAGACTCGGTACCCCCAGCAGTA
CCAGCCCTCGCCACTCCCTGTGCATTATGAGCTGCTCACAGATGGATACACCCGGC
mm4__chr2 88.12 Mbp_chr2_88122514 TCCTCTACCTTCTGCCCCTGCCCATAGCATGGAGGGAACTTCCACTGAGCCTCCTCTGTCCACTTCTGGCGCCTCTCACT
AAGCCCTTTGCTCAGCCTCCCACAGGAGGAAAAGTTTATTGGGCGGGTCAGTGTC
mm4__chr2 130.73 Mbp_chr2_130727814 GATGATGGTGGTCCGGGGCTGTGGTGGGCGTGGCCCCGGGCTGCGGCGGGCGTGGTCCCCGGGCTGCGGCGGACGTGGTC
AGGGGCGGTTCTGGGCGTGGTCCCTGGGCTGCGGTGGACGTGGTCTCGGGCTGTGACGAG
mm4__chr21 44.63 Mbp_chr21_44626421 AAAGATGCTTGCAGTGCAGGGCACACGGAGGAGATGGCTCCAGCAGTGGCGAGGGCTGGCGGCTGCCTGCTGCCTAGCAC
AGCTACTTCACGGG
mm4__chr11 45.89 Mbp_chr11_45891093 CTGGCTCCTGATGGACCTTGGATGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTAAAGTTCGTCTCTGGATTGCTGG
mm3__chr17 40.94 Mbp_chr17_40936531 GTGAAGGACAGGGAGATGCGGGCTCCCCCCGCACCGCCATGGACGGTGGGAGCCCTGGGGAAGCTCCTGGGCCGGCCCCA
GCCACCTCCGGCGCCATGGAAGGAGGCCGAGGGGGTCTGGCTGAAGCTGTGTCCGGAGTT
mm4__chr20 2.17 Mbp_chr20_2172658 CCTCTGCCACACTTGCAAACACAGACACACACTTCTCCACCCCTGGACACGTCCTGCGCGGGAGCTCCGAGATGCGTGAG
CGCCTGTAACCAGGGATGCTGCCGCCCCCTCCTGGAAAGATTGGG
mm4__chr1 110.03 Mbp_chr1_110034690 GGCCCACTGATCGTGAACAACAAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNGGGCCTCCTGCCTGGGGGCAAAGCCCGCGAGTTCAAC
mm4__chr7 47.98 Mbp_chr7_47979351 CTTCATCCCCACAAGTGCCCTCCGACCCGCCCCGCGCCCTCCCGGCCCCACCCTCCCGCGGCCTACACCAGCACCCCCAT
CCCGGCCCCATCCTCCCGCGGCCCCTTCCGGCGCCCATCCCCGTTCTGATCCTC
mm4__chr6 165.86 Mbp_chr6_165855291 AATGAACAGAGGCGGATGGGTGGAGCAGGTGCTGGTGAGGGCACCAGCCTTCGAGGGGACTCATAGGCGGCGCGGGAAGT
GGCGGGGGGGGGGGGGGACTCAGGGGCGCTGCCTCCTGTGGATCACCTGGTAGC
mm4__chr9 97.52 Mbp_chr9_97517576 ATGCTGGGTTGGGCATGTGGGGCCTGAGGACTCCAGGGGGAGGTACCCAGAAGGGAGCAGGGGCTCAGGAAGGGGCCTGG
GCTGGATAGACACCATCTGAGTGTCCTCAGCTTATCACAGGTAGCAGGCCC
mm4__chr9 96.56 Mbp_chr9_96560216 TTCACGCTGTAGTCCTCGTGCCTGGCTCCTCAAGACCCTACCATCTCAGGGACATCTCATCTGACCAGAGACAGCAATTG
ACCCGGAAGTGGCCCGGTCTGGCCCCTCTGCTTTGGAGTTCT
mm4__chr8 93.74 Mbp_chr8_93740817 CCGTTCTTCCGGCTTGGTCGCCCAGCCCTCCCCACCCCCGGCGCCCCGAGGGGAACTGCTTCGCACTTGGCAATAAACAC
ACACCACTGCTGCCGAGTCGGACACACAAGGCCGGAGGCTGAGGGAACTCTTCG
mm4__chr7 44.23 Mbp_chr7_44229411 AGCAGGACCCAGGCTACTTACATGGGGCTGGCAGGGGGCTAAAGGGAGCCGGAGATGGGCAGGGCAGGGGCCCCTCGGCT
TCTGGGGCTCCCGAGCCCCTCCTCACTGAGCTCAGGATGTCGG
mm4__chr6 169.70 Mbp_chr6_169702772 CCAGCTTCGACGACTACGAGCTGCAATCGGCCACCTGCGGCCCGGACGCCGTGTCCATCCCCGCGCACTTCCGGCGCCCA
GTGGGCATCGGCGTCTATGGACACCCCTCCCA
mm4__chr5 173.06 Mbp_chr5_173057267 CGGATGGAGACTTCTGCACCAATGGGCGTGGGGGAGGCGGTGCCGCGGGGGGGCCACTAATTGGTTAGGCTGTGCCGGAG
GTGGGCCGGGCGGGGCAGCCATGTTAGATGGGAGGGGACCAGTG
mm4__chr4 185.21 Mbp_chr4_185210287 GTAAGTACCCGACTCCTGGCCGCCCAGCTCCGCCGGCCCTCCCCGCTTCCGGTGCCAGCTCCCGCGCCCCGAGCTCCGGG
GGGCCGCGGCATGCCCTTGTCGAAGCGGGAAGCC
mm4__chr3 54.92 Mbp_chr3_54918787 CTGCGAGGACACTGGATCAGGAGCAGGAAGAGGGCAGGGCTGGTACAGCTCATGAGGGATCCTAAGGAGGTCCTTTCCCT
TCCAGGTGTCTGGTGATTCACAGATGATGCCGTCTGTTAGTCCCC
mm4__chr3 43.11 Mbp_chr3_43105614 GAGGGCTCGAACTGGCAAGCCCCAGGGCGTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNTGAGGGTCCACCCCCGCCCCGCCCACCTCCCGCGCCTCCCTCAGCTCGCTTCAC
mm4__chr3 141.05 Mbp_chr3_141051227 GGCTCCACTTCCCTCTCCCGCCAGCTGGTGGCCTCGAGAAGGTGGCGGCGCCGGCAGCGGCCCGAGCTGGGACGGCCGGG
GCGCGGGATGGTGGGGGAAGGGGCGTTCCCGGCGACCGCGCCTTCCGCGGCTATTGGATT
mm4__chr3 120.35 Mbp_chr3_120349343 GGAACCTGAACCGAGGGCTGAGTCGGAAGTGGCGCGGGCGGGCGCAAACCCTGCGCGCCTTG
mm4__chr22 43.68 Mbp_chr22_43682072 TTTCCCATGACTGCCCTGAGCAGCCAGGCAAAACGCCCAGCCCTGCCCACTCCTGGCTCCCACCCTTATTCTCCTTCTTA
CCTAGTCCCCTCCGTAGTGGGGTGCATGGTGGGGGGATGGGAGGGGAGGGGAATCTCACT
mm4__chr20 61.40 Mbp_chr20_61400835 CCTCCTTGTTGTTCTGGCCTTTCTATTTCCACCGGCCCTGCCCCCTTCCAGAGCCTCATCCTTTCATCTGCACCTTGTGA
GCTCCTAACTACTTTCTAAGTTGACGTCCCTTTGCCACTGTAG
mm4__chr2 238.80 Mbp_chr2_238796507 CTGCAGGTCCCCTTCATGTCCGTGCCTGCCTCTCTGTCCCTGGACCACCCCTGCCCTGCCCACTCCCTGCTCCACATCAG
CTTCTCACTAAGTCCTGGTTCCCTCCCATCCTCCTGTAACCTTTCCCTTCTGTGCCC
mm4__chr19 831.91 Kbp_chr19_831907 GCCTGTGACCTCCCATGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNAGAGGGGCCCTGGCTGTAATAAAGAAGCCGATCTCTCCTCTGC
mm4__chr17 75.73 Mbp_chr17_75731007 TCAATGTGAGGGCAGCTCAGGCTCCAGAGTCTGAGCCCTTCTGGGGCAGGGCAGGAAGTGGGCAGGGTGGGCAAGAGGTG
TCTTGGATCACGGTGGAGAAATGGGTCTGGG
mm4__chr15 74.37 Mbp_chr15_74369587 ACTTGTGGTCCTCTTGGCAGCCACCCCAACTTCCGTTCAGGCCTCCGCACCTCCCCCAGTGTTGTGGGGCACTGACCGCC
TCCCCAGAAGTGGCCCGGGCTGGAGGTTATTGCCTCATCCTCTCCAATGGCCATGGTCAC
mm4__chr13 40.64 Mbp_chr13_40635789 CAGGAAAGGCCTCTGGATGGTCAGGCACACCACTGAGGAGAGTGAGTCCTCTCCCCTCCACTGAGGGCCTGCCCTGCCCA
CTTCTGGGGACGTGACAGAGGTCCCAGCCATCAGGATGAGCTGTACTCTACCTGCTGCC
mm4__chr11 68.27 Mbp_chr11_68272126 GTTGTCTGCTCTCAGGCCCCGCCTCCCCGGGGCCGGCTCGGGCCACTTCTTGCGCGCTGCCAGAAGGAGCTATTTTGAGC
AGGTCTGTGGCG
mm4__chr10 8.58 Mbp_chr10_8584817 GCCAGGGCAATGGGACAAATACTTGGGTACAGGAGCACTTAGACCAGTCCAGGCCACTTCCTGTGCAAGTCTGGGAACTG
CATTCCTTCACGTGCT
mm4__chr1 88.53 Mbp_chr1_88529558 ACCAGGATCTAACTTTTGGCTCAGAAGTTTCCATCCCAGGCCACTTCAGCCGCAAAACATTTCAATTCTATTATTTAAGG
AATGGTTTGAAATATCCCCCAATTCAGCTTAACACCTCCCATAGCACATTTCTGC
mm4__chr1 3.42 Mbp_chr1_3418850 CGCTGACCCACTCCTAGGAGTAAGTATGCCATTCCCAGGGCTCCCTGCTGGAGTGAGCAGGCAGTGGGCAGGGCCGGGTG
CTGAATTCTGGTCACTCTGGCCCTCCCCTCCTGGCCTCAGCAGGATGTTTGA
mm4__chr1 229.63 Mbp_chr1_229626476 CTGGTCCAGAGGAGATCCCCCTACCTAAGGCCGGGCCCAGATGGGGGGCCCCCCAACCCTGCCCAGTTCCGGTGCCGCCA
GCAGACTCTGGGCTGGAGCGGCTTCGGCACGCTCCATTCGCGAAGGCGAACTCCCTCCTG
mm4__chr1 22.96 Mbp_chr1_22956715 TTTCAGAGCAATCCCTCCGCACCTGCCTTGCTCTGTTCCTGATCCCGCCCTGCCCAATTCCCGTGCCCCAGCTTCATCTT
CCAGTGCCAGCCTGCTCCTGCCCCCAACCTTGTCGACTTCA
mm4__chr1 11.68 Mbp_chr1_11681424 CGAGGGCATTTCTCTCGCTTTAGCAGGCGCCGGAAGAGGGCTGGGGAGGGACGGGGGAGCGCGCAGCTGGGGACTAACGG
CAGCCACATCTGAGCTAGCCCGAG
mm3__chr1 2.47 Mbp_chr1_2466592 CATGGCAGGAGGTGGTCACACCTTGAGGTGGCAGCGGCTCAGAGAGCCCAGAGGCGGCCCCACATCCTCCTGCCCGGNNN
NNNNNNNNNNNNNNNNNNNNNCACATCCGGGGCTCAGTGTCCACACAAGCCTC

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_gYvicTzp9e5VPFC9YwLR.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.