CRISPOR

Micro-homology scoring of potential deletions

Guide: ATGTGCAAGGCCGGCTTCGC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	(Wild-type sequence)
382.8	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGC---------GACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`9 bp deleted → no frameshift`
327.6	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGC----GACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`4 bp deleted → frameshift`
313.2	`TCGTCGACAACGGCTCCGGCATGTGCAAGGC-------------GACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`13 bp deleted → frameshift`
296.4	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCG------ACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`6 bp deleted → no frameshift`
268.0	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGG--------GCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`8 bp deleted → frameshift`
255.2	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCG---------ATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`9 bp deleted → no frameshift`
229.6	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGG-------------------------GCCGTCTTCCCCTCCATCGTG`	`25 bp deleted → frameshift`
223.0	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCG------------------------------TCTTCCCCTCCATCGTG`	`30 bp deleted → no frameshift`
219.6	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGC------------CCCCCGGGCCGTCTTCCCCTCCATCGTG`	`12 bp deleted → no frameshift`
219.6	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCG------------ACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`12 bp deleted → no frameshift`
219.6	`TCGTCGACAACGGCTCCGGCATGTGCAAGG------------GCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`12 bp deleted → no frameshift`
210.0	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCC---------------------CCCCGGGCCGTCTTCCCCTCCATCGTG`	`21 bp deleted → no frameshift`
188.8	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCG---------------ATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`15 bp deleted → no frameshift`
180.6	`TCGTCGACAACGGCTCCGGC------------------------GACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`24 bp deleted → no frameshift`
170.8	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGC-----------------CCCCCGGGCCGTCTTCCCCTCCATCGTG`	`17 bp deleted → frameshift`
163.8	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGC--------------------------CGTCTTCCCCTCCATCGTG`	`26 bp deleted → frameshift`
162.8	`TCGTCGACAACGGCTCCGGCATGTGC------------------GACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`18 bp deleted → no frameshift`
154.8	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCG-------------------GGCCGTCTTCCCCTCCATCGTG`	`19 bp deleted → frameshift`
140.0	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGC---------------------CGTCTTCCCCTCCATCGTG`	`21 bp deleted → no frameshift`
136.5	`TCGTCGACAACGGCTCCGGCATGTGC--------------------------CCCCCGGGCCGTCTTCCCCTCCATCGTG`	`26 bp deleted → frameshift`
133.8	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTC------------------------------CCCTCCATCGTG`	`30 bp deleted → no frameshift`
133.8	`TCGTCGACAACGGC------------------------------GACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`30 bp deleted → no frameshift`
133.2	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCC----------------------CCCGGGCCGTCTTCCCCTCCATCGTG`	`22 bp deleted → frameshift`
126.8	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCC-----------------------CCGGGCCGTCTTCCCCTCCATCGTG`	`23 bp deleted → frameshift`
126.8	`TCGTCGACAACGGCTCCGG-----------------------GCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`23 bp deleted → frameshift`
120.4	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCG------------------------TCTTCCCCTCCATCGTG`	`24 bp deleted → no frameshift`
120.4	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCC------------------------CGGGCCGTCTTCCCCTCCATCGTG`	`24 bp deleted → no frameshift`
108.0	`TCGTCGACAACGGCTCCGG----------------------------------------GCCGTCTTCCCCTCCATCGTG`	`40 bp deleted → frameshift`
103.6	`TCGTCGACAACGGCTCCG---------------------------ACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`27 bp deleted → no frameshift`
99.4	`TCGTCGAC---------------------------------------GATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`39 bp deleted → no frameshift`
98.8	`TCGTCGACAACGGCTCCGGCATG----------------------------CCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`28 bp deleted → frameshift`
94.0	`TCGTCGACAACGGCTCCGGCATGTGCAAGG-----------------------------GCCGTCTTCCCCTCCATCGTG`	`29 bp deleted → frameshift`
94.0	`TCGTCGACAACGG-----------------------------GCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`29 bp deleted → frameshift`
89.2	`TCGTCGACAACGGCTCCG------------------------------ATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`30 bp deleted → no frameshift`
82.5	`TCGTCGACAACG------------------------------------ATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`36 bp deleted → no frameshift`
80.8	`TCGTCGACAACGGCTCCGGC--------------------------------CCCCCGGGCCGTCTTCCCCTCCATCGTG`	`32 bp deleted → frameshift`
77.7	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTC---------------------------TTCCCCTCCATCGTG`	`27 bp deleted → no frameshift`
77.4	`TCGTCGACAACGGCTCCGGC-----------------------------------------CGTCTTCCCCTCCATCGTG`	`41 bp deleted → frameshift`
76.8	`TCGTCGACAACG---------------------------------ACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`33 bp deleted → no frameshift`
71.0	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCG---------------------------------------TG`	`39 bp deleted → no frameshift`
69.6	`TCGTCGACAACGGCTCCGGCATGTGC-----------------------------------CGTCTTCCCCTCCATCGTG`	`35 bp deleted → frameshift`
66.0	`TCGTCGACAACGGCTCC------------------------------------CCCCGGGCCGTCTTCCCCTCCATCGTG`	`36 bp deleted → no frameshift`
63.0	`TCGTCGACAACGGCTCCG---------------------------------------------TCTTCCCCTCCATCGTG`	`45 bp deleted → no frameshift`
62.8	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCC-------------------------------------CCTCCATCGTG`	`37 bp deleted → frameshift`
62.8	`TCGTCGACAACGGCTCC-------------------------------------CCCGGGCCGTCTTCCCCTCCATCGTG`	`37 bp deleted → frameshift`
61.0	`TCGTCGA------------------------------------------TGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`42 bp deleted → no frameshift`
60.0	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCC--------------------------------------CTCCATCGTG`	`38 bp deleted → frameshift`
60.0	`TCGTCGACAACGGCTCC--------------------------------------CCGGGCCGTCTTCCCCTCCATCGTG`	`38 bp deleted → frameshift`
60.0	`TCGTCGACAACGGC--------------------------------------CCCCCGGGCCGTCTTCCCCTCCATCGTG`	`38 bp deleted → frameshift`
60.0	`TCGTCGACAACGG----------------------------------------------GCCGTCTTCCCCTCCATCGTG`	`46 bp deleted → frameshift`
57.0	`TCGTCGACAACGGC-----------------------------------------------CGTCTTCCCCTCCATCGTG`	`47 bp deleted → frameshift`
56.8	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCC---------------------------------------TCCATCGTG`	`39 bp deleted → no frameshift`
56.8	`TCGTCGACAACGGCTCC---------------------------------------CGGGCCGTCTTCCCCTCCATCGTG`	`39 bp deleted → no frameshift`
52.2	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTC-----------------------------------CATCGTG`	`35 bp deleted → frameshift`
49.5	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCT------------------------------------CCATCGTG`	`36 bp deleted → no frameshift`
48.8	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCC------------------------------------------ATCGTG`	`42 bp deleted → no frameshift`
48.8	`TCG------------------------------------------ACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`42 bp deleted → no frameshift`
42.0	`TCGTCGACAACGGCTCCGGCATGTGCAAGGCCG---------------------------------------------TG`	`45 bp deleted → no frameshift`
42.0	`TCG---------------------------------------------ATGCCCCCCGGGCCGTCTTCCCCTCCATCGTG`	`45 bp deleted → no frameshift`
40.6	`TCGTCGACAACGGCTCC---------------------------------------------------------ATCGTG`	`57 bp deleted → no frameshift`
40.5	`TCGTCGACAACGGCTCCGGCATGT----------------------------------------CTTCCCCTCCATCGTG`	`40 bp deleted → frameshift`
37.0	`TCGTCGACAACGGCTCC----------------------------------------------------CCTCCATCGTG`	`52 bp deleted → frameshift`
35.0	`TCGTC------------------------------------------------------------TTCCCCTCCATCGTG`	`60 bp deleted → no frameshift`
32.0	`TCGTCGACAACGGCTCCGGCATGTG-------------------------------------------------------`	`55 bp deleted → frameshift`
31.2	`TCGTCGACAACG---------------------------------------------------TCTTCCCCTCCATCGTG`	`51 bp deleted → no frameshift`
29.6	`TCGTCG----------------------------------------------------GGCCGTCTTCCCCTCCATCGTG`	`52 bp deleted → frameshift`
28.4	`TCGTCGACAACGGCTCC-----------------------------------------------------CTCCATCGTG`	`53 bp deleted → frameshift`
27.3	`TCGTCGACAACGGCTCCGGCATGTGCA------------------------------------------------TCGTG`	`48 bp deleted → no frameshift`
26.8	`TCGTCGACAACGGCTCCGGCAT------------------------------------------------------CGTG`	`54 bp deleted → no frameshift`
26.8	`TCGTCGACAACGGCTCC------------------------------------------------------TCCATCGTG`	`54 bp deleted → no frameshift`
25.8	`TCGTCGACAACGGCTC-------------------------------------------------TTCCCCTCCATCGTG`	`49 bp deleted → frameshift`
25.6	`TCG-------------------------------------------------------GGCCGTCTTCCCCTCCATCGTG`	`55 bp deleted → frameshift`
23.4	`TCGTCGACAACGGCT---------------------------------------------------TCCCCTCCATCGTG`	`51 bp deleted → no frameshift`
23.2	`TCGTCG---------------------------------------------------------TCTTCCCCTCCATCGTG`	`57 bp deleted → no frameshift`
20.0	`TCGTCGACAACGGCTCCG------------------------------------------------------------TG`	`60 bp deleted → no frameshift`
17.4	`TCGTCGACAACGGCTCCGGCATG---------------------------------------------------------`	`57 bp deleted → no frameshift`
14.8	`TCGTCGACAACG------------------------------------------------------------------TG`	`66 bp deleted → no frameshift`
14.4	`TCGT---------------------------------------------------------------------------G`	`75 bp deleted → no frameshift`
14.1	`TCGTCGACAACGGCTC-------------------------------------------------------------GTG`	`61 bp deleted → frameshift`
13.5	`TCGTCG------------------------------------------------------------------------TG`	`72 bp deleted → no frameshift`
12.9	`TCGTC---------------------------------------------------------------CCCTCCATCGTG`	`63 bp deleted → no frameshift`
12.9	`TC---------------------------------------------------------------TTCCCCTCCATCGTG`	`63 bp deleted → no frameshift`
11.1	`TCGTCGACA------------------------------------------------------------------TCGTG`	`66 bp deleted → no frameshift`
11.1	`TC------------------------------------------------------------------CCCTCCATCGTG`	`66 bp deleted → no frameshift`
9.9	`TCGTC--------------------------------------------------------------------CATCGTG`	`68 bp deleted → frameshift`
8.7	`TC-----------------------------------------------------------------------CATCGTG`	`71 bp deleted → frameshift`

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