CRISPOR

Micro-homology scoring of potential deletions

Guide: CGCGCCCAGATTGGGGACAA AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	(Wild-type sequence)
352.5	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGA-------AGCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`7 bp deleted → frameshift`
283.2	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGG---------------CCGGCCGCGTTATTACCATAAAAGGCAAA`	`15 bp deleted → no frameshift`
269.4	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGG----------------CCGGCCGCGTTATTACCATAAAAGGCAAA`	`16 bp deleted → frameshift`
268.0	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGG--------AAGCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`8 bp deleted → frameshift`
255.2	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGG---------AAGCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`9 bp deleted → no frameshift`
202.0	`GCCGCCAGGGGGCGCCGG--------------------------------GCCGGCCGCGTTATTACCATAAAAGGCAAA`	`32 bp deleted → frameshift`
199.8	`GCCGCCAGGGGGCGCCGGCGCGCGCC----------------------GGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`22 bp deleted → frameshift`
198.8	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGG--------------GCCGGCCGCGTTATTACCATAAAAGGCAAA`	`14 bp deleted → frameshift`
188.4	`GCCGCCAGGGGGCGCCGGC-------------------------------------CGCGTTATTACCATAAAAGGCAAA`	`37 bp deleted → frameshift`
170.8	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGG-----------------CCGGCCGCGTTATTACCATAAAAGGCAAA`	`17 bp deleted → frameshift`
156.6	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAG-------------GAAGCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`13 bp deleted → frameshift`
155.8	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAA-----GCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`5 bp deleted → frameshift`
155.4	`GCCGCCAGGGGGCGCCGGCGCGCGCC---------------------------GGCCGCGTTATTACCATAAAAGGCAAA`	`27 bp deleted → no frameshift`
154.8	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGG-------------------CCGCGTTATTACCATAAAAGGCAAA`	`19 bp deleted → frameshift`
149.1	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGA--------------AGCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`14 bp deleted → frameshift`
147.2	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGG--------------------CCGCGTTATTACCATAAAAGGCAAA`	`20 bp deleted → frameshift`
140.0	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGG---------------------CCGCGTTATTACCATAAAAGGCAAA`	`21 bp deleted → no frameshift`
140.0	`GCCGCCAGGGGGCGCCGGCGCGCGCCC---------------------GGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`21 bp deleted → no frameshift`
132.0	`GCCGCCAGGGGGCGCCGGCGCGCG------------------------------------TTATTACCATAAAAGGCAAA`	`36 bp deleted → no frameshift`
128.1	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAG-----------------CCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`17 bp deleted → frameshift`
127.2	`GCCGCCAGGGGGCGCCGGCGCGCGCC-------------------------------GCGTTATTACCATAAAAGGCAAA`	`31 bp deleted → frameshift`
120.4	`GCCGCCAGGGGGCGCCGGCGCGC------------------------CGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`24 bp deleted → no frameshift`
120.0	`GCCGCCAGGGGGCGCCGGCGCG--------------------------------------TTATTACCATAAAAGGCAAA`	`38 bp deleted → frameshift`
115.2	`GCCGCCAGGGGGCGCCGGC---------------------------------CGGCCGCGTTATTACCATAAAAGGCAAA`	`33 bp deleted → no frameshift`
114.8	`GCCGCCAGGGGGCGCCGGCGCGCG-------------------------GGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`25 bp deleted → frameshift`
114.8	`GCCGCCAGGGGGCGCCGG-------------------------AAGCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`25 bp deleted → frameshift`
113.6	`GCCGCCAGGGGGC---------------------------------------CGGCCGCGTTATTACCATAAAAGGCAAA`	`39 bp deleted → no frameshift`
109.8	`GCCGCCAGGGGGCGCCGGCGCGCGC----------------------------------GTTATTACCATAAAAGGCAAA`	`34 bp deleted → frameshift`
109.2	`GCCGCCAGGGGGCGCCGGCGCGCGCCC--------------------------GGCCGCGTTATTACCATAAAAGGCAAA`	`26 bp deleted → frameshift`
109.2	`GCCGCCAGGGGGCGCCGGCGC--------------------------CGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`26 bp deleted → frameshift`
103.6	`GCCGCCAGGGGGCGCCGGCGCG---------------------------GGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`27 bp deleted → no frameshift`
103.2	`GCCGCCAGGGGGCGCCG-----------------------------------------CGTTATTACCATAAAAGGCAAA`	`41 bp deleted → frameshift`
98.8	`GCCGCCAGGGGGCGCCGGC----------------------------CGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`28 bp deleted → frameshift`
94.0	`GCCGCCAGGGGGCGCCGGCGCGC-----------------------------CGGCCGCGTTATTACCATAAAAGGCAAA`	`29 bp deleted → frameshift`
94.0	`GCCGCCAGGGGGCGCCGGCG-----------------------------GGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`29 bp deleted → frameshift`
91.5	`GCCGCCAGG----------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`34 bp deleted → frameshift`
89.2	`GCCGCCAGGGGGCGCCGGCGCGCGCCC------------------------------GCGTTATTACCATAAAAGGCAAA`	`30 bp deleted → no frameshift`
89.2	`GCCGCCAGGGGGCGCCGGCGCGCG------------------------------GCCGCGTTATTACCATAAAAGGCAAA`	`30 bp deleted → no frameshift`
84.8	`GCCGCCAGGGGGCGCCGGCGC-------------------------------CGGCCGCGTTATTACCATAAAAGGCAAA`	`31 bp deleted → frameshift`
84.8	`GCCGCCAGGGGG-------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`31 bp deleted → frameshift`
84.0	`GCCG---------------------------------------------GGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`45 bp deleted → no frameshift`
81.0	`GCCGCCAGGGGGCGCCGGCG----------------------------------------TTATTACCATAAAAGGCAAA`	`40 bp deleted → frameshift`
81.0	`GCCGCCAGGGG----------------------------------------CCGGCCGCGTTATTACCATAAAAGGCAAA`	`40 bp deleted → frameshift`
80.8	`GCCGCCAGGGGGCGCCGGCGCG--------------------------------GCCGCGTTATTACCATAAAAGGCAAA`	`32 bp deleted → frameshift`
80.8	`GCCGCCAGGGG--------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`32 bp deleted → frameshift`
77.4	`GCCGCCAGGG-----------------------------------------CCGGCCGCGTTATTACCATAAAAGGCAAA`	`41 bp deleted → frameshift`
76.8	`GCCGCCAGGGGGCGCCGGCGCGC---------------------------------CGCGTTATTACCATAAAAGGCAAA`	`33 bp deleted → no frameshift`
76.8	`GCCGCCAGGG---------------------------------AAGCCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`33 bp deleted → no frameshift`
73.2	`GCCGCCAGGGGGCGCCGGCG----------------------------------GCCGCGTTATTACCATAAAAGGCAAA`	`34 bp deleted → frameshift`
73.2	`GCCGCCAGGGGGC----------------------------------CGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`34 bp deleted → frameshift`
73.2	`GCCGCC------------------------------------------GGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`42 bp deleted → no frameshift`
70.5	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGAC-----------------------------CATAAAAGGCAAA`	`29 bp deleted → frameshift`
69.6	`GCCGCCAGGGGGC-------------------------------------------CGCGTTATTACCATAAAAGGCAAA`	`43 bp deleted → frameshift`
69.6	`GCCGCCAGGGGGCGCCGGCGC-----------------------------------CGCGTTATTACCATAAAAGGCAAA`	`35 bp deleted → frameshift`
69.6	`GCCGCCAGGGGGCG-----------------------------------GGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`35 bp deleted → frameshift`
67.0	`GCCGC------------------------------------------------------GTTATTACCATAAAAGGCAAA`	`54 bp deleted → no frameshift`
66.9	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACA------------------------------TAAAAGGCAAA`	`30 bp deleted → no frameshift`
66.6	`GCCGCCAGGGGGCGC--------------------------------------------GTTATTACCATAAAAGGCAAA`	`44 bp deleted → frameshift`
65.6	`GCCG--------------------------------------------------GCCGCGTTATTACCATAAAAGGCAAA`	`50 bp deleted → frameshift`
64.5	`GCCGCCAGGGGGCGCCGGCGCGCGCCCA-----------------------------------------TAAAAGGCAAA`	`41 bp deleted → frameshift`
60.0	`GCCGCCAGGGGGCG----------------------------------------------TTATTACCATAAAAGGCAAA`	`46 bp deleted → frameshift`
60.0	`GCCGCCAGGGGG--------------------------------------GCCGGCCGCGTTATTACCATAAAAGGCAAA`	`38 bp deleted → frameshift`
57.6	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATT---------------------------------ACCATAAAAGGCAAA`	`33 bp deleted → no frameshift`
57.0	`GCCGCC-----------------------------------------------GGCCGCGTTATTACCATAAAAGGCAAA`	`47 bp deleted → frameshift`
56.8	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAA---------------------------------------A`	`39 bp deleted → no frameshift`
54.0	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGG----------------------------------------CAAA`	`40 bp deleted → frameshift`
54.0	`GCCGCCAGGGGGCG----------------------------------------GCCGCGTTATTACCATAAAAGGCAAA`	`40 bp deleted → frameshift`
51.6	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGG-----------------------------------------CAAA`	`41 bp deleted → frameshift`
48.8	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGG------------------------------------------CAAA`	`42 bp deleted → no frameshift`
48.8	`GCCGCCAGGGGGCGCCGGCGCGCGCC------------------------------------------ATAAAAGGCAAA`	`42 bp deleted → no frameshift`
48.8	`GCCGCCAGG------------------------------------------CCGGCCGCGTTATTACCATAAAAGGCAAA`	`42 bp deleted → no frameshift`
46.8	`GCCGCC---------------------------------------------------GCGTTATTACCATAAAAGGCAAA`	`51 bp deleted → no frameshift`
46.4	`GCCGCCAGGGGGCGCCG-------------------------------------------TTATTACCATAAAAGGCAAA`	`43 bp deleted → frameshift`
45.0	`GCCGCCAG--------------------------------------CCGGGCCGGCCGCGTTATTACCATAAAAGGCAAA`	`38 bp deleted → frameshift`
44.6	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATT------------------------------ATTACCATAAAAGGCAAA`	`30 bp deleted → no frameshift`
44.4	`GCCGCCAGGGG--------------------------------------------CCGCGTTATTACCATAAAAGGCAAA`	`44 bp deleted → frameshift`
42.0	`GCCGCCAGGG---------------------------------------------CCGCGTTATTACCATAAAAGGCAAA`	`45 bp deleted → no frameshift`
40.4	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAA--------------------------------AAGGCAAA`	`32 bp deleted → frameshift`
40.0	`GCCGCCAGG----------------------------------------------CCGCGTTATTACCATAAAAGGCAAA`	`46 bp deleted → frameshift`
38.4	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAA---------------------------------AGGCAAA`	`33 bp deleted → no frameshift`
36.6	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAA----------------------------------GGCAAA`	`34 bp deleted → frameshift`
33.0	`GCCGCCAGGGGGCGCCGGC----------------------------------------------------------AAA`	`58 bp deleted → frameshift`
30.0	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAG----------------------------------------------GCAAA`	`46 bp deleted → frameshift`
29.6	`GCCGCCAGGGGGCGCCGGCGCGCGC----------------------------------------------------AAA`	`52 bp deleted → frameshift`
29.6	`GCCGCCAGGGGGCGCC----------------------------------------------------ATAAAAGGCAAA`	`52 bp deleted → frameshift`
28.4	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGAT---------------------------------------AAAAGGCAAA`	`39 bp deleted → no frameshift`
27.0	`GCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAA----------------------------------------`	`40 bp deleted → frameshift`
26.8	`GCCGCCAGGGGGCGCCGGCGCGC------------------------------------------------------AAA`	`54 bp deleted → no frameshift`
24.6	`GCCGCCAGGGGGCGCCGGCGCGCGCCCA--------------------------------------------------AA`	`50 bp deleted → frameshift`
24.6	`GCCGCCAGGGGGC----------------------------------------------------------------AAA`	`64 bp deleted → frameshift`
24.4	`GCCGCCAGGGGGCGCCGGCGC--------------------------------------------------------AAA`	`56 bp deleted → frameshift`
24.4	`GCCG--------------------------------------------------------TTATTACCATAAAAGGCAAA`	`56 bp deleted → frameshift`
23.2	`GC---------------------------------------------------------GTTATTACCATAAAAGGCAAA`	`57 bp deleted → no frameshift`
22.5	`GCCGCCA--------------------------------------------------------------TAAAAGGCAAA`	`62 bp deleted → frameshift`
18.0	`GCCGCCAGGGGGCGC--------------------------------------------------------------AAA`	`62 bp deleted → frameshift`
17.5	`GCCGCCAGG-------------------------------------------------------------------CAAA`	`67 bp deleted → frameshift`
15.6	`GCCGCCAGGGG-----------------------------------------------------------------CAAA`	`65 bp deleted → frameshift`
15.6	`GCC-----------------------------------------------------------------ATAAAAGGCAAA`	`65 bp deleted → frameshift`
14.8	`GCCGCCAGGG------------------------------------------------------------------CAAA`	`66 bp deleted → no frameshift`
10.8	`GCCGC------------------------------------------------------------------------AAA`	`72 bp deleted → no frameshift`
9.6	`GC---------------------------------------------------------------------------AAA`	`75 bp deleted → no frameshift`
8.7	`GCCGCCA-----------------------------------------------------------------------AA`	`71 bp deleted → frameshift`

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