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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529656_F | TCGTCGGCAGCGTCAGCGCGGCGATATCATCATC | 60.8 | 1.00 |
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529656_R | GTCTCGTGGGCTCGGCTGGCGGCCTAAGGACTC | 59.4 | 1.00 |
| mm4__chr4 87.87 Mbp_chr4_87872225_F | TCGTCGGCAGCGTCTCCCCTTCCCTGCATATTGC | 59.8 | 0.42 |
| mm4__chr4 87.87 Mbp_chr4_87872225_R | GTCTCGTGGGCTCGGTGGTGTTGTTAGCTTTCCACCT | 60.0 | 0.42 |
| mm4__chr17 33.02 Mbp_chr17_33016032_F | TCGTCGGCAGCGTCACATTCTCATCGTGGCTCCC | 59.8 | 0.29 |
| mm4__chr17 33.02 Mbp_chr17_33016032_R | GTCTCGTGGGCTCGGCTGCCTCTGCACCCTCTTC | 60.0 | 0.29 |
| mm3__chr4 27.56 Mbp_chr4_27555717_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm3__chr4 27.56 Mbp_chr4_27555717_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4__chr6 161.57 Mbp_chr6_161568024_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4__chr6 161.57 Mbp_chr6_161568024_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm3__chr12 94.88 Mbp_chr12_94875211_F | TCGTCGGCAGCGTCTGTGCCGGGTAAACACTTCT | 59.5 | 0.21 |
| mm3__chr12 94.88 Mbp_chr12_94875211_R | GTCTCGTGGGCTCGGACGGTCTCTATAGTGCTGCTG | 59.3 | 0.21 |
| mm4__chr2 36.58 Mbp_chr2_36583411_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr2 36.58 Mbp_chr2_36583411_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr12 130.83 Mbp_chr12_130833508_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr12 130.83 Mbp_chr12_130833508_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr10 44.47 Mbp_chr10_44467539_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr10 44.47 Mbp_chr10_44467539_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr22 40.96 Mbp_chr22_40960014_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr22 40.96 Mbp_chr22_40960014_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr3 196.83 Mbp_chr3_196825242_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr3 196.83 Mbp_chr3_196825242_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr6 106.81 Mbp_chr6_106808488_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4__chr6 106.81 Mbp_chr6_106808488_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3__chr14 55.75 Mbp_chr14_55747677_F | TCGTCGGCAGCGTCTGTTTCTGCCTGTCTTGCCT | 59.8 | 0.15 |
| mm3__chr14 55.75 Mbp_chr14_55747677_R | GTCTCGTGGGCTCGGTTTTGTAAAAGGGAAAGGTGGC | 57.5 | 0.15 |
| mm4__chr4 90.07 Mbp_chr4_90073250_F | TCGTCGGCAGCGTCTTTGTCTCCTTTTCTTTTCTTTGGA | 57.5 | 0.15 |
| mm4__chr4 90.07 Mbp_chr4_90073250_R | GTCTCGTGGGCTCGGAGCCTCATCTTTAGCGATGTGA | 59.5 | 0.15 |
| mm4__chr18 45.67 Mbp_chr18_45665271_F | TCGTCGGCAGCGTCACTCATGAGTGCAGAACGGG | 60.0 | 0.15 |
| mm4__chr18 45.67 Mbp_chr18_45665271_R | GTCTCGTGGGCTCGGTTCAGGCCCCTGGAAGTTTC | 59.8 | 0.15 |
| mm4__chr9 130.97 Mbp_chr9_130974227_F | TCGTCGGCAGCGTCCTACCCAGGTGCAATCCCC | 59.7 | 0.15 |
| mm4__chr9 130.97 Mbp_chr9_130974227_R | GTCTCGTGGGCTCGGTGAGCAGAAGTCCCTGAGGT | 60.1 | 0.15 |
| mm4__chr2 36.34 Mbp_chr2_36335946_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4__chr2 36.34 Mbp_chr2_36335946_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4__chr7 124.93 Mbp_chr7_124930046_F | TCGTCGGCAGCGTCCTGACAGGATGCGACGTCAA | 60.3 | 0.14 |
| mm4__chr7 124.93 Mbp_chr7_124930046_R | GTCTCGTGGGCTCGGAAGCTGGAAAGTCACTGGGG | 59.8 | 0.14 |
| mm4__chr16 75.80 Mbp_chr16_75800241_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr16 75.80 Mbp_chr16_75800241_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr7 104.24 Mbp_chr7_104241671_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr7 104.24 Mbp_chr7_104241671_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr5 78.52 Mbp_chr5_78515956_F | TCGTCGGCAGCGTCCATGATGCCTCCCAGGTCAC | 60.4 | 0.13 |
| mm4__chr5 78.52 Mbp_chr5_78515956_R | GTCTCGTGGGCTCGGTGGGCAGATCGTTTCATGCT | 60.0 | 0.13 |
| mm3__chr5 51.00 Mbp_chr5_50995170_F | TCGTCGGCAGCGTCCCACAAATGCATTGCAGCCA | 60.3 | 0.13 |
| mm3__chr5 51.00 Mbp_chr5_50995170_R | GTCTCGTGGGCTCGGTCACCAAGGAATGTGCCCTC | 59.9 | 0.13 |
| mm4__chr10 981.68 Kbp_chr10_981681_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr10 981.68 Kbp_chr10_981681_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3__chr3 176.15 Mbp_chr3_176151410_F | TCGTCGGCAGCGTCTGTGGCTCAAAGCTCTACCA | 58.9 | 0.12 |
| mm3__chr3 176.15 Mbp_chr3_176151410_R | GTCTCGTGGGCTCGGGGGCAACTCTCCTTGCTGAA | 60.2 | 0.12 |
| mm4__chr3 59.92 Mbp_chr3_59918917_F | TCGTCGGCAGCGTCAAGCAAAGCCCAAGGACCAT | 60.1 | 0.12 |
| mm4__chr3 59.92 Mbp_chr3_59918917_R | GTCTCGTGGGCTCGGTTTTCTCATTCCCAGCCTACTT | 57.3 | 0.12 |
| mm4__chr3 165.18 Mbp_chr3_165177210_F | TCGTCGGCAGCGTCACATGGAGAAGCTTTTCCTGGA | 59.6 | 0.12 |
| mm4__chr3 165.18 Mbp_chr3_165177210_R | GTCTCGTGGGCTCGGCCCCTGTAAAAGTCAGTAAACCT | 58.0 | 0.12 |
| mm4__chr9 134.60 Mbp_chr9_134603054_F | TCGTCGGCAGCGTCTCACCGCCCCTTTCTTCTTG | 60.2 | 0.11 |
| mm4__chr9 134.60 Mbp_chr9_134603054_R | GTCTCGTGGGCTCGGCAGAACACGGAGCTGACCC | 60.3 | 0.11 |
| mm4__chr17 59.47 Mbp_chr17_59468735_F | TCGTCGGCAGCGTCGTCTGGAGAGCTAAGCAGGG | 59.5 | 0.11 |
| mm4__chr17 59.47 Mbp_chr17_59468735_R | GTCTCGTGGGCTCGGCTCCAGTGCAGCAGCTCAG | 60.7 | 0.11 |
| mm4__chr8 65.62 Mbp_chr8_65620994_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr8 65.62 Mbp_chr8_65620994_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr10 91.17 Mbp_chr10_91171614_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr10 91.17 Mbp_chr10_91171614_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4__chr3 187.29 Mbp_chr3_187287268_F | TCGTCGGCAGCGTCTCATCTCAGTAGCCAGCCCT | 60.0 | 0.10 |
| mm4__chr3 187.29 Mbp_chr3_187287268_R | GTCTCGTGGGCTCGGTGCAATGAGGCTGGAATGGA | 59.6 | 0.10 |
| mm3__chr6 51.62 Mbp_chr6_51620532_F | TCGTCGGCAGCGTCACTTGTCCTAAACCCTGGGG | 58.9 | 0.10 |
| mm3__chr6 51.62 Mbp_chr6_51620532_R | GTCTCGTGGGCTCGGTCTTCAATACATACAAATGCGTGGT | 59.3 | 0.10 |
| mm2__chr15 49.86 Mbp_chr15_49856997_F | TCGTCGGCAGCGTCCCACAGGGTTTAGCAGCTCT | 59.6 | 0.10 |
| mm2__chr15 49.86 Mbp_chr15_49856997_R | GTCTCGTGGGCTCGGGACTAGCCCCTGATGCACTG | 60.1 | 0.10 |
| mm4__chr1 180.26 Mbp_chr1_180259847_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr1 180.26 Mbp_chr1_180259847_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr12 17.51 Mbp_chr12_17505667_F | TCGTCGGCAGCGTCTTCGAACATGGGGCTGCATA | 59.7 | 0.10 |
| mm4__chr12 17.51 Mbp_chr12_17505667_R | GTCTCGTGGGCTCGGTGCACAAGGTACATTATCATGCAA | 59.2 | 0.10 |
| mm3__chr6 161.78 Mbp_chr6_161775887_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3__chr6 161.78 Mbp_chr6_161775887_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr1 107.27 Mbp_chr1_107273210_F | TCGTCGGCAGCGTCATCAGGCCTTGCTAGATGGC | 59.8 | 0.09 |
| mm4__chr1 107.27 Mbp_chr1_107273210_R | GTCTCGTGGGCTCGGTCTCCACTGCATCACTCAGC | 59.7 | 0.09 |
| mm4__chr6 134.00 Mbp_chr6_133995291_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr6 134.00 Mbp_chr6_133995291_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr16 6.44 Mbp_chr16_6440886_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr16 6.44 Mbp_chr16_6440886_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr18 22.17 Mbp_chr18_22167235_F | TCGTCGGCAGCGTCAATCGGTCAATGTCCACCCC | 60.0 | 0.08 |
| mm4__chr18 22.17 Mbp_chr18_22167235_R | GTCTCGTGGGCTCGGGTCTGCGTGGATATCCCTGG | 59.9 | 0.08 |
| mm4__chr6 137.95 Mbp_chr6_137948118_F | TCGTCGGCAGCGTCGGGAAGGTGTTACTGATGGCA | 59.9 | 0.08 |
| mm4__chr6 137.95 Mbp_chr6_137948118_R | GTCTCGTGGGCTCGGCTGTGAGGCATCTTCCCAGG | 60.1 | 0.08 |
| mm4__chr14 93.97 Mbp_chr14_93968584_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr14 93.97 Mbp_chr14_93968584_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr1 163.91 Mbp_chr1_163910682_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr1 163.91 Mbp_chr1_163910682_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr18 39.14 Mbp_chr18_39144633_F | TCGTCGGCAGCGTCATGAAAATATTTCCCACATCCCTC | 57.0 | 0.07 |
| mm4__chr18 39.14 Mbp_chr18_39144633_R | GTCTCGTGGGCTCGGAATGTTTGTTATGTGGTCAAGCT | 57.2 | 0.07 |
| mm4__chr7 128.00 Mbp_chr7_127997743_F | TCGTCGGCAGCGTCACTCACTTCGTAATTCGTGCCT | 60.0 | 0.07 |
| mm4__chr7 128.00 Mbp_chr7_127997743_R | GTCTCGTGGGCTCGGTGCTGGGCCGAAAGTGTAAG | 60.6 | 0.07 |
| mm4__chrX 50.37 Mbp_chrX_50372782_F | TCGTCGGCAGCGTCTGGGACCATCTTCGACTCAG | 58.8 | 0.07 |
| mm4__chrX 50.37 Mbp_chrX_50372782_R | GTCTCGTGGGCTCGGGCTGTAAGCATGTGAGGGCT | 60.3 | 0.07 |
| mm4__chr10 98.44 Mbp_chr10_98436560_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr10 98.44 Mbp_chr10_98436560_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrY 3.29 Mbp_chrY_3286024_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrY 3.29 Mbp_chrY_3286024_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrX 89.63 Mbp_chrX_89629845_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrX 89.63 Mbp_chrX_89629845_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr2 433.43 Kbp_chr2_433433_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr2 433.43 Kbp_chr2_433433_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr11 4.61 Mbp_chr11_4607150_F | TCGTCGGCAGCGTCGCAAACCCCTCGTCCATTCA | 60.6 | 0.06 |
| mm4__chr11 4.61 Mbp_chr11_4607150_R | GTCTCGTGGGCTCGGGGCAAATGACGTCAAAGGCA | 59.6 | 0.06 |
| mm4__chrX 126.10 Mbp_chrX_126099406_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chrX 126.10 Mbp_chrX_126099406_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr7 4.81 Mbp_chr7_4814573_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr7 4.81 Mbp_chr7_4814573_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr13 30.72 Mbp_chr13_30718812_F | TCGTCGGCAGCGTCTGAGGTCATGACAGCCCTCT | 60.2 | 0.06 |
| mm4__chr13 30.72 Mbp_chr13_30718812_R | GTCTCGTGGGCTCGGAGGGAGTGTCCAGGAGTTCA | 59.8 | 0.06 |
| mm4__chr2 156.64 Mbp_chr2_156638888_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr2 156.64 Mbp_chr2_156638888_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr19 44.98 Mbp_chr19_44983514_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr19 44.98 Mbp_chr19_44983514_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr18 61.79 Mbp_chr18_61794612_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr18 61.79 Mbp_chr18_61794612_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr10 62.29 Mbp_chr10_62289238_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr10 62.29 Mbp_chr10_62289238_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr1 93.32 Mbp_chr1_93316474_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr1 93.32 Mbp_chr1_93316474_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr16 28.62 Mbp_chr16_28619477_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr16 28.62 Mbp_chr16_28619477_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr12 63.51 Mbp_chr12_63506126_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr12 63.51 Mbp_chr12_63506126_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr15 89.70 Mbp_chr15_89695455_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr15 89.70 Mbp_chr15_89695455_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr16 28.07 Mbp_chr16_28072850_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr16 28.07 Mbp_chr16_28072850_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr17 7.72 Mbp_chr17_7715862_F | TCGTCGGCAGCGTCCGTGGAGATGGCTCTGAGAA | 59.1 | 0.05 |
| mm4__chr17 7.72 Mbp_chr17_7715862_R | GTCTCGTGGGCTCGGCATTACCCTCCGAACCCAGG | 59.8 | 0.05 |
| mm4__chr14 50.52 Mbp_chr14_50515400_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr14 50.52 Mbp_chr14_50515400_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr6 151.46 Mbp_chr6_151456748_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr6 151.46 Mbp_chr6_151456748_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr1 29.95 Mbp_chr1_29950783_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr1 29.95 Mbp_chr1_29950783_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3__chr12 95.54 Mbp_chr12_95536140_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3__chr12 95.54 Mbp_chr12_95536140_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr3 122.23 Mbp_chr3_122233433_F | TCGTCGGCAGCGTCGGTCTTTGGCTGGATGGAGT | 59.6 | 0.04 |
| mm4__chr3 122.23 Mbp_chr3_122233433_R | GTCTCGTGGGCTCGGTTCTCCTCCGCTACTTCCCA | 59.9 | 0.04 |
| mm4__chr4 43.34 Mbp_chr4_43344658_F | TCGTCGGCAGCGTCTCCACCACATCCAGTTCTGC | 59.9 | 0.04 |
| mm4__chr4 43.34 Mbp_chr4_43344658_R | GTCTCGTGGGCTCGGGGAGGGTTGCTTGGAAAAGG | 59.0 | 0.04 |
| mm4__chr12 96.90 Mbp_chr12_96900097_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr12 96.90 Mbp_chr12_96900097_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 41.39 Mbp_chr7_41387633_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 41.39 Mbp_chr7_41387633_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr5 110.34 Mbp_chr5_110335265_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr5 110.34 Mbp_chr5_110335265_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chrX 120.21 Mbp_chrX_120208254_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chrX 120.21 Mbp_chrX_120208254_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr18 59.56 Mbp_chr18_59561163_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr18 59.56 Mbp_chr18_59561163_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr1 116.46 Mbp_chr1_116463785_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr1 116.46 Mbp_chr1_116463785_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr17 7.51 Mbp_chr17_7513647_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr17 7.51 Mbp_chr17_7513647_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr14 41.50 Mbp_chr14_41503104_F | TCGTCGGCAGCGTCACTTTCTCAAGGCTCATGAAGTC | 58.6 | 0.04 |
| mm4__chr14 41.50 Mbp_chr14_41503104_R | GTCTCGTGGGCTCGGGGAAAGATGGCAATTTGAGGCT | 59.5 | 0.04 |
| mm4__chr19 15.14 Mbp_chr19_15141813_F | TCGTCGGCAGCGTCGACAGCCAGGGATGGAGATG | 59.8 | 0.03 |
| mm4__chr19 15.14 Mbp_chr19_15141813_R | GTCTCGTGGGCTCGGGCCACACTCCCATGTACCAC | 60.6 | 0.03 |
| mm4__chr1 158.09 Mbp_chr1_158094250_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr1 158.09 Mbp_chr1_158094250_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr5 1.05 Mbp_chr5_1052067_F | TCGTCGGCAGCGTCTCCAGAAACCAAGGCAAGGG | 60.1 | 0.03 |
| mm4__chr5 1.05 Mbp_chr5_1052067_R | GTCTCGTGGGCTCGGTTCCTTGCATCTGAGGGCAG | 60.0 | 0.03 |
| mm4__chr8 8.26 Mbp_chr8_8262366_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr8 8.26 Mbp_chr8_8262366_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr5 1.38 Mbp_chr5_1382191_F | TCGTCGGCAGCGTCAGAGTAGAAGGGAGGCCTCG | 60.1 | 0.03 |
| mm4__chr5 1.38 Mbp_chr5_1382191_R | GTCTCGTGGGCTCGGCTCCCTGCTACTGTCGGAGA | 60.3 | 0.03 |
| mm4__chr10 5.96 Mbp_chr10_5958483_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr10 5.96 Mbp_chr10_5958483_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chrX 117.25 Mbp_chrX_117246365_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chrX 117.25 Mbp_chrX_117246365_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr7 87.94 Mbp_chr7_87935430_F | TCGTCGGCAGCGTCAAATAACCACAGGCCGAGGG | 60.0 | 0.02 |
| mm4__chr7 87.94 Mbp_chr7_87935430_R | GTCTCGTGGGCTCGGCATGCCTGGCTTTTGCTTGA | 59.6 | 0.02 |
| mm4__chr11 129.42 Mbp_chr11_129424530_F | TCGTCGGCAGCGTCTCCATCCCTTCCTTCCCCTC | 60.3 | 0.02 |
| mm4__chr11 129.42 Mbp_chr11_129424530_R | GTCTCGTGGGCTCGGACATGTGGCAAAGGCTGAGT | 60.1 | 0.02 |
| mm4__chr1 35.57 Mbp_chr1_35574497_F | TCGTCGGCAGCGTCCTGGAGGCAGAAGGTGACAA | 59.6 | 0.02 |
| mm4__chr1 35.57 Mbp_chr1_35574497_R | GTCTCGTGGGCTCGGATCCTCGGAGTCACCAGTGA | 59.9 | 0.02 |
| mm4__chr8 79.94 Mbp_chr8_79941331_F | TCGTCGGCAGCGTCCCTTCCTTGATTCCAAATGTGGT | 59.1 | 0.02 |
| mm4__chr8 79.94 Mbp_chr8_79941331_R | GTCTCGTGGGCTCGGTGCCATTTGGAGCACTTTCT | 58.0 | 0.02 |
| mm4__chr9 137.01 Mbp_chr9_137005738_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr9 137.01 Mbp_chr9_137005738_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chrX 38.32 Mbp_chrX_38321556_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chrX 38.32 Mbp_chrX_38321556_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr16 60.66 Mbp_chr16_60655663_F | TCGTCGGCAGCGTCTTGCCACCATAACAGCACCA | 60.1 | 0.02 |
| mm4__chr16 60.66 Mbp_chr16_60655663_R | GTCTCGTGGGCTCGGGTGCCAGTTATAGCAGCAGGA | 60.1 | 0.02 |
| mm4__chr9 127.54 Mbp_chr9_127542317_F | TCGTCGGCAGCGTCTACAGTCTTCCGCCAAGCTC | 59.7 | 0.02 |
| mm4__chr9 127.54 Mbp_chr9_127542317_R | GTCTCGTGGGCTCGGGACCTTTACCCACCAGGAGG | 59.3 | 0.02 |
| mm4__chr1 152.92 Mbp_chr1_152924451_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr1 152.92 Mbp_chr1_152924451_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr11 66.42 Mbp_chr11_66417363_F | TCGTCGGCAGCGTCTGTGCCCACACCAGGAATTT | 60.1 | 0.02 |
| mm4__chr11 66.42 Mbp_chr11_66417363_R | GTCTCGTGGGCTCGGGCCTGCAGGAGTAAGTGGTT | 59.9 | 0.02 |
| mm4__chr2 64.90 Mbp_chr2_64900344_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr2 64.90 Mbp_chr2_64900344_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4__chr7 141.21 Mbp_chr7_141212154_F | TCGTCGGCAGCGTCTGAGGATGGGAGGATGGAGG | 60.1 | 0.02 |
| mm4__chr7 141.21 Mbp_chr7_141212154_R | GTCTCGTGGGCTCGGTGGGCACACACAATTTTCTGG | 59.5 | 0.02 |
| mm4__chr14 96.64 Mbp_chr14_96637871_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr14 96.64 Mbp_chr14_96637871_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr21 45.75 Mbp_chr21_45748858_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr21 45.75 Mbp_chr21_45748858_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr6 3.24 Mbp_chr6_3240764_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr6 3.24 Mbp_chr6_3240764_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr11 132.65 Mbp_chr11_132646200_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr11 132.65 Mbp_chr11_132646200_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr14 105.78 Mbp_chr14_105775005_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr14 105.78 Mbp_chr14_105775005_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3__chr9 26.71 Mbp_chr9_26709993_F | TCGTCGGCAGCGTCGCCCTACTGACACTTTTGCA | 58.3 | 0.00 |
| mm3__chr9 26.71 Mbp_chr9_26709993_R | GTCTCGTGGGCTCGGTCGTTTGTGGTGGTGGAACT | 59.7 | 0.00 |
| mm4__chr1 230.07 Mbp_chr1_230068290_F | TCGTCGGCAGCGTCCCTGGGCATTCGGGAGGA | 61.0 | 0.00 |
| mm4__chr1 230.07 Mbp_chr1_230068290_R | GTCTCGTGGGCTCGGACACTACAAACGTCCCCTGC | 60.2 | 0.00 |
| mm4__chr12 38.08 Mbp_chr12_38084711_F | TCGTCGGCAGCGTCATCTCCCTCTTCCCTGCCTT | 59.9 | 0.00 |
| mm4__chr12 38.08 Mbp_chr12_38084711_R | GTCTCGTGGGCTCGGCTCTGCCTGGGAAGCTGTC | 60.0 | 0.00 |
| mm4__chr3 8.81 Mbp_chr3_8814689_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr3 8.81 Mbp_chr3_8814689_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr17 63.98 Mbp_chr17_63981120_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr17 63.98 Mbp_chr17_63981120_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr7 1.49 Mbp_chr7_1487772_F | TCGTCGGCAGCGTCCTCCTTCAGCCTCTGTCCCA | 60.9 | 0.00 |
| mm4__chr7 1.49 Mbp_chr7_1487772_R | GTCTCGTGGGCTCGGGACCATCACTGAGAGCAGCA | 59.7 | 0.00 |
| mm4__chr6 6.78 Mbp_chr6_6776626_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr6 6.78 Mbp_chr6_6776626_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 34.41 Mbp_chr5_34407437_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 34.41 Mbp_chr5_34407437_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 20.80 Mbp_chr5_20796463_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr5 20.80 Mbp_chr5_20796463_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr4 3.22 Mbp_chr4_3219525_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr4 3.22 Mbp_chr4_3219525_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr3 15.60 Mbp_chr3_15599567_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr3 15.60 Mbp_chr3_15599567_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr19 58.36 Mbp_chr19_58362296_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr19 58.36 Mbp_chr19_58362296_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr19 31.84 Mbp_chr19_31840293_F | TCGTCGGCAGCGTCATGCTTCTTGCTACACCCGG | 60.3 | 0.00 |
| mm4__chr19 31.84 Mbp_chr19_31840293_R | GTCTCGTGGGCTCGGCACTTTCAGGGGTCAGGGAC | 59.9 | 0.00 |
| mm4__chr18 79.70 Mbp_chr18_79696056_F | TCGTCGGCAGCGTCTAAGAACGGGAAGCAGCAGG | 60.0 | 0.00 |
| mm4__chr18 79.70 Mbp_chr18_79696056_R | GTCTCGTGGGCTCGGCTCCTCAGAGCACCACGC | 60.1 | 0.00 |
| mm4__chr17 48.47 Mbp_chr17_48472695_F | TCGTCGGCAGCGTCGGCAGCTTAGCACTCTGGTT | 60.3 | 0.00 |
| mm4__chr17 48.47 Mbp_chr17_48472695_R | GTCTCGTGGGCTCGGTGGCCTCACTTTGTCACACC | 60.4 | 0.00 |
| mm4__chr15 22.79 Mbp_chr15_22789478_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr15 22.79 Mbp_chr15_22789478_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr11 58.64 Mbp_chr11_58640526_F | TCGTCGGCAGCGTCATACCAACAGGAGGAGGGCA | 60.2 | 0.00 |
| mm4__chr11 58.64 Mbp_chr11_58640526_R | GTCTCGTGGGCTCGGGGGATGTGAGTACTGGGCAC | 60.1 | 0.00 |
| mm4__chr1 244.30 Mbp_chr1_244303994_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr1 244.30 Mbp_chr1_244303994_R | Primer3: not found at this Tm | N.d. | 0.00 |
Primers underlined, off-targets in bold.
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529656 |
AGCGCGGCGATATCATCATCCATGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCC ACTTCCGGCGCGCCGAGTCCTTAGGCCGCCAG |
| mm4__chr4 87.87 Mbp_chr4_87872225 |
TCCCCTTCCCTGCATATTGCTTCAACTTCTTGCTCTTTCCTTTGCTAAATATAACTAGCTTCCTGCTATTTTCGCAGCTG ACTGAAGAATTTCATTAGGAAGGTGGAAAGCTAACAACACCA |
| mm4__chr17 33.02 Mbp_chr17_33016032 |
ACATTCTCATCGTGGCTCCCTTCGTCCTCCTCTTTGCCAAGCAGGTCTAATAGCTTCTCTTTGATCAGCTGCAAGAAAAG CAGGAAGGGGTTGGTCAGGCCGGGAAGAGGGTGCAGAGGCAG |
| mm3__chr12 94.88 Mbp_chr12_94875211 |
TGTGCCGGGTAAACACTTCTCATGAGTGTTTCAGCTTTGGCATTGCCTTTATGCCAGTTATTCTGGCAGATCACCACTGC CAAAGAAATACCTTTATAATTTTCTAACATTGATTGTCAGCAGCACTATAGAGACCGT |
| mm3__chr14 55.75 Mbp_chr14_55747677 |
TGTTTCTGCCTGTCTTGCCTTCTTTCTTCATTCTTCCATCTATTCTCGCAGCTCCACAGCCACCTTTCCCTTTTACAAAA |
| mm4__chr4 90.07 Mbp_chr4_90073250 |
TTTGTCTCCTTTTCTTTTCTTTGGATATAGAAAACCTGCAATTCTGGCAGCTGATCATAAAAGTGAAGGAGATAAGTTAA TATAAAATGTTTTCTCAGTAAATAGAACTGTGTGCTCACATCGCTAAAGATGAGGCT |
| mm4__chr18 45.67 Mbp_chr18_45665271 |
ACTCATGAGTGCAGAACGGGGGAACAGGCAATTCAGTCTGAAAACACAGGGTAGTCAGAGCCCAGCCTGCTATTCTCACA CTTGACCACAGGAGGGAACCAAAGCATCAAGAAGGAAACTTCCAGGGGCCTGAA |
| mm4__chr9 130.97 Mbp_chr9_130974227 |
CTACCCAGGTGCAATCCCCACCCAGGCCATGGACCCTGCAATTCTGGCTGCTCCCCACACCTTCCCAATCCCTCTCATTC CTCTCCAACCTCAGGGACTTCTGCTCA |
| mm4__chr7 124.93 Mbp_chr7_124930046 |
CTGACAGGATGCGACGTCAACGGGAGCGCAGGGCCAAGGTACAGGCCCCGCCCAGTCCGGTCACATCCCCCGGAATTCTC GCAGCTCCCCTTAGCTCCGCCCCCAGTGACTTTCCAGCTT |
| mm4__chr5 78.52 Mbp_chr5_78515956 |
CATGATGCCTCCCAGGTCACAGGGCCCAGTGACTGTAGGGAAACCAGCTGTTCTCCCAGCTCATCTGTTGTCTGATAATC CTCGTACAGCATGAAACGATCTGCCCA |
| mm3__chr5 51.00 Mbp_chr5_50995170 |
CCACAAATGCATTGCAGCCAATGCCTAAATCCCAAACCCACCTTGGTGAGCTGCAAGTACAGCTGGAATGCTAGACCATG TGAACGCTAAGGACAAGAACAGGCAACCGAGGGCACATTCCTTGGTGA |
| mm3__chr3 176.15 Mbp_chr3_176151410 |
TGTGGCTCAAAGCTCTACCAGCTACTCTCACCTGCTATTCTCACAGCTTTCCAACAGTTATGGTACAATAGGCATTAGCT CTTTAATATAAATTTATACCAAATCACAACTTCTATTCAGCAAGGAGAGTTGCCC |
| mm4__chr3 59.92 Mbp_chr3_59918917 | AAGCAAAGCCCAAGGACCATCAATATGCAATGCTGAGCTGCAGGAAAAGCAGGAAGTAGGCTGGGAATGAGAAAA |
| mm4__chr3 165.18 Mbp_chr3_165177210 |
ACATGGAGAAGCTTTTCCTGGAAGTTATTAATTATCACCAGGTATTCTCTCAGCCCAACACAGTTAATTATTCAGAAAAA TTCAAGTTAAAAGGTTTACTGACTTTTACAGGGG |
| mm4__chr9 134.60 Mbp_chr9_134603054 |
TCACCGCCCCTTTCTTCTTGGAGGAAAAGCCTGCTGGTGTGGTGCGAGGATGGCGGGTGTTCCACCCTGCCCTGGGCCAC CAACACCACGGCCAGGGCGGCCCCCCTGCAAGCACCGAGGGGGTCAGCTCCGTGTTCTG |
| mm4__chr17 59.47 Mbp_chr17_59468735 |
GTCTGGAGAGCTAAGCAGGGCCTGACACTCACCTACAAGGACCTTCCATTCCCACGTGGTCCTTCTGTTCTGGCAGCTCC CCAGTGCTGAGCTGCTGCACTGGAG |
| mm4__chr3 187.29 Mbp_chr3_187287268 | TCATCTCAGTAGCCAGCCCTGTGAGCCCCCTGTTATTCTGGGAGCACACCATGCCCTTCCATTCCAGCCTCATTGCA |
| mm3__chr6 51.62 Mbp_chr6_51620532 |
ACTTGTCCTAAACCCTGGGGGGTAGATGGTAATACTGTCATCTACTTTTACTACGTTTACTGTCCCTGCTTTTCTCACAT CTCACCACCAAATACATTAACCACGCATTTGTATGTATTGAAGA |
| mm2__chr15 49.86 Mbp_chr15_49856997 | CCACAGGGTTTAGCAGCTCTGTGGTGAGCTGAGAGAAAAGCCAGAGACAGCAGTGCATCAGGGGCTAGTC |
| mm4__chr12 17.51 Mbp_chr12_17505667 |
TTCGAACATGGGGCTGCATAATTATGGTGATGTACGAGAATAGTTGGTTTGTCCTTTTCAACCTTCACTAACATTCTTTT GCATGATAATGTACCTTGTGCA |
| mm4__chr1 107.27 Mbp_chr1_107273210 |
ATCAGGCCTTGCTAGATGGCATGAATTACTCAGAAGCTTACAGACATCAAGGTAGGGGGGTCCTGAAAGGTGTGGCAGGG GTCGCTGAGCAGTGAGAATAGCCGGGAAGTGAGTGCTGAGTGATGCAGTGGAGA |
| mm4__chr18 22.17 Mbp_chr18_22167235 |
AATCGGTCAATGTCCACCCCGCTGCTCACCTCTGTCCCAGCAGCGCCGGGGCCAGCGCGCCCTCCCGCCGCGTCTGCGGA GCTGCGGGAAAAGCAGGTCCCCGGGGGGTATCGAGTGTCCAGGGATATCCACGCAGAC |
| mm4__chr6 137.95 Mbp_chr6_137948118 |
GGGAAGGTGTTACTGATGGCATAAATAGGGTTTTTGAACAGAGGTGACTCTAAAAAAAATGAACTCATGTTGTGCTGAGT GAATAGCTGGATATTCCTGGGAAGATGCCTCACAG |
| mm4__chr18 39.14 Mbp_chr18_39144633 |
ATGAAAATATTTCCCACATCCCTCCCTGCCTTTCTAGTAGCTCACCAATACAGTTGCATTTACATATTTAATTCTGTTTT GAAACTTTCTGCTTATAAAATTGTATAAATAGCTTGACCACATAACAAACATT |
| mm4__chr7 128.00 Mbp_chr7_127997743 |
ACTCACTTCGTAATTCGTGCCTTATCAGAATGGACCTGTTATACTGGCAGTTCACCATATATACCATTACATTTTGTTTC TCCCATCTCTCAGGTAGACTTACACTTTCGGCCCAGCA |
| mm4__chrX 50.37 Mbp_chrX_50372782 |
TGGGACCATCTTCGACTCAGAAGAATCAAGTTCTAGAACATCAACACAGGTGAGATGGTTCACATTCCAGTAAGTAGTGA GCTGGGAGAACAGATGGGCAAAGATATCTTCAGCCCTCACATGCTTACAGC |
| mm4__chr11 4.61 Mbp_chr11_4607150 |
GCAAACCCCTCGTCCATTCAATCTACCTGACTTGGGTGCCTGCTATTCTCCCAGCTGAAAATGACTTCTACATGATCCTG CCTTTGACGTCATTTGCC |
| mm4__chr13 30.72 Mbp_chr13_30718812 | TGAGGTCATGACAGCCCTCTCCTGCTATGCTCATAGCTGACCACTGAACTCCTGGACACTCCCT |
| mm4__chr17 7.72 Mbp_chr17_7715862 |
CGTGGAGATGGCTCTGAGAAATCAAATATTGACAATGAGGGAACAGAACTTATTAAATCTGGGACAGGGATGTGTGTGGG GAGCTGTGGGAATGGCCGGATGCCTGGGTTCGGAGGGTAATG |
| mm4__chr3 122.23 Mbp_chr3_122233433 |
GGTCTTTGGCTGGATGGAGTTGACATCAGTCCCCTTCCCTGCTCTTCTCTAATCTCACCACAGGTACCCTGCTCTGGGCC TCAGGGTGTTGGGGAGTAGAGCTGGGAAGTAGCGGAGGAGAA |
| mm4__chr4 43.34 Mbp_chr4_43344658 |
TCCACCACATCCAGTTCTGCAGTATCCTTCCGATGTCATTTGTCTGTAGTTCATCAGCCCCCGGCTTTTCTCCCAGCTCA AGATCCTTTTCCAAGCAACCCTCC |
| mm4__chr14 41.50 Mbp_chr14_41503104 |
ACTTTCTCAAGGCTCATGAAGTCTAGTGGTGAGCTGCAAGTATACAGGGTAACGTATAATAAGCACTAGAAGTATCAAAG TTGATGATACTACTGACTTTTTAAACAGTTTTAAAATAGCCTCAAATTGCCATCTTTCC |
| mm4__chr19 15.14 Mbp_chr19_15141813 |
GACAGCCAGGGATGGAGATGGCCTGGTCTCCATCAGCAGATGAGCCAGCATGCTGGGGATGGTGACCAGCGTGTATAGCA GGTCTCCGAGAGGGACAGCACCACCAGGAAGTGGTACATGGGAGTGTGGC |
| mm4__chr5 1.05 Mbp_chr5_1052067 |
TCCAGAAACCAAGGCAAGGGCTCACTGGCTTCTTGTGACCTGCGAGAAGATCTGGCCACGTCCAGGTCACTCCGGTAGCA GCGTCTGCCCTCAGATGCAAGGAA |
| mm4__chr5 1.38 Mbp_chr5_1382191 |
AGAGTAGAAGGGAGGCCTCGGAGGGAGGTGGTCTCCAAGAGTAGCCGGGAGGCCTGGGAGGATGGTGGTCTCCGAGAGTA GCAGGGAGGCCTGGGAGGGTGGTGGTCTCCGACAGTAGCAGGGAG |
| mm4__chr7 87.94 Mbp_chr7_87935430 |
AAATAACCACAGGCCGAGGGCTTTGCCCGAGGGGAGTTTGTGCGGGGTGCCTTCAGCAGTGCGGTGAGCTGAGAGAACAG GCGGAGAGCCCTCTGACATCTGTCAAGCAAAAGCCAGGCATG |
| mm4__chr11 129.42 Mbp_chr11_129424530 |
TCCATCCCTTCCTTCCCCTCTTGGAACTCAGTTCCTGCTCTTTTCACAGCTCACCCTGTCACCAGGCACTGTACTCTCTA GAATTCCTACACTTCCTACACCACTCAGCCTTTGCCACATGT |
| mm4__chr1 35.57 Mbp_chr1_35574497 | CTGGAGGCAGAAGGTGACAAATGCAGGAAGCCGACTTTTCTCCCAGCTCGCCACATCTCACTGGTGACTCCGAGGAT |
| mm4__chr8 79.94 Mbp_chr8_79941331 |
CCTTCCTTGATTCCAAATGTGGTATTACAACATCTTGATCCATTCATTGCTCAGTCTCCTGCTACTCTTCCAGATCACCA ACTGAAGAAAGTGCTCCAAATGGCA |
| mm4__chr16 60.66 Mbp_chr16_60655663 |
TTGCCACCATAACAGCACCACATGAAAAAGGCACCTGACATTCACACAGCTCACCAACTGGTTTCATTTTCAGGAAGTCC ATTCCTGCTGCTATAACTGGCAC |
| mm4__chr9 127.54 Mbp_chr9_127542317 |
TACAGTCTTCCGCCAAGCTCACTCCTCAGCCCACCACCATGCGTCTTTCAACTGGAAAGCCCCAGGCTGGGTGGGAGCCC TCCACCTATTCCCGCAGCCCACCGCTGGCCTCCTGGTGGGTAAAGGTC |
| mm4__chr11 66.42 Mbp_chr11_66417363 |
TGTGCCCACACCAGGAATTTTTTTTTTTTAGAACTAAAGAAAGCCCTTCTCTTCTCTCAGCTCTCCAAATATGGAGTGAT GGAAAACCACTTACTCCTGCAGGC |
| mm4__chr7 141.21 Mbp_chr7_141212154 | TGAGGATGGGAGGATGGAGGCCCCCACCCACCATTCCCCCAGCTCACCATTCCAGAAAATTGTGTGTGCCCA |
| mm3__chr9 26.71 Mbp_chr9_26709993 |
GCCCTACTGACACTTTTGCAATTATCAATACCACCACTACCACTTTCTCCATCACTTCCTCACTTCCAATCCCAGCTCTG CTCCCAGCTCACCATCTTCACCTCTAAAACCTCCATTACTAGTTCCACCACCACAAACGA |
| mm4__chr1 230.07 Mbp_chr1_230068290 |
CCTGGGCATTCGGGAGGATGGTGAGCGCCGAGAGGAGCCGGGACAGCGCGGTCCTGCGGCCGAGCGCCGGCCGGGAGAGC AGGGCGTCGGGCTGTGTGCTGGAGCATGCCGGCAGGGGACGTTTGTAGTGT |
| mm4__chr12 38.08 Mbp_chr12_38084711 |
ATCTCCCTCTTCCCTGCCTTCCACAAGGTCCAGCACCACCCATTCTCCCAGGTCACCACTCCATTCCAGGTGTGCAGCTG GACAGCTTCCCAGGCAGAG |
| mm4__chr7 1.49 Mbp_chr7_1487772 |
CTCCTTCAGCCTCTGTCCCACCCACACCAACCACCCACAGGTCCCGACGGCAGGCGCAGGGCGGGGCTGTGTGGGCTGCG TACTGGGGGTCCAGGCTGGTGAGCTGCGACAGGAGGAGGCTGCTGCTCTCAGTGATGGTC |
| mm4__chr19 31.84 Mbp_chr19_31840293 |
ATGCTTCTTGCTACACCCGGGCCCTGGCCTGCTGCCCCTTGTTTATCGGCCAGCAAATCTGGCAACTCACCACCCTAGGA GTATGAGAGCACAATATGTGAGGTCCCTGACCCCTGAAAGTG |
| mm4__chr18 79.70 Mbp_chr18_79696056 |
TAAGAACGGGAAGCAGCAGGTGCCGCTGTCCACGGCGACCGTGTCCATGGTGCACAGCGTGCCGGAGTTGATGGTGAGCT CCGAGGTGAGCCGGGCATCAGTGGCGGCGTGTTGGGGAAGCGTGGTGCTCTGAGGAG |
| mm4__chr17 48.47 Mbp_chr17_48472695 |
GGCAGCTTAGCACTCTGGTTTTTATGTGTGAAGCCGTAATATTTGGTGAGCTGTGAGATGAGGTGGCAAGATTTGCAACT GGCATTCCCTTCCGGCCCTGCCCTGTGATTCAGGTCAGTGGTGTGACAAAGTGAGGCCA |
| mm4__chr11 58.64 Mbp_chr11_58640526 |
ATACCAACAGGAGGAGGGCAAGGAAAACTCTCTGAGGATGTTTTGTTTTCAGCCCTGGGCAGCATTTGCTGAGCTGGGAG ATTAGGAGGCATCATTTGGTGCCCAGTACTCACATCCC |