← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529727_F | TCGTCGGCAGCGTCTGGTGAGCTGCGAGAATAGC | 60.1 | 1.00 |
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529727_R | GTCTCGTGGGCTCGGCGGCTTCCTTTGTCCCCAAT | 60.6 | 1.00 |
| mm4__chr18 21.90 Mbp_chr18_21903971_F | TCGTCGGCAGCGTCGCTGGCCTCTTGTTCACTCA | 60.2 | 0.58 |
| mm4__chr18 21.90 Mbp_chr18_21903971_R | GTCTCGTGGGCTCGGCCGTGATCTCAAAGGCCCC | 60.4 | 0.58 |
| mm4__chr4 5.14 Mbp_chr4_5142948_F | Primer3: not found at this Tm | N.d. | 0.48 |
| mm4__chr4 5.14 Mbp_chr4_5142948_R | Primer3: not found at this Tm | N.d. | 0.48 |
| mm3__chr1 240.00 Mbp_chr1_239997993_F | Primer3: not found at this Tm | N.d. | 0.43 |
| mm3__chr1 240.00 Mbp_chr1_239997993_R | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4__chr14 95.05 Mbp_chr14_95049101_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4__chr14 95.05 Mbp_chr14_95049101_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4__chr6 98.83 Mbp_chr6_98832745_F | TCGTCGGCAGCGTCCTGTGTGTGTCCTCGCCTC | 60.3 | 0.31 |
| mm4__chr6 98.83 Mbp_chr6_98832745_R | GTCTCGTGGGCTCGGGTTTATGAGCGTGTGTGGGG | 59.1 | 0.31 |
| mm4__chr13 27.38 Mbp_chr13_27383836_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr13 27.38 Mbp_chr13_27383836_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr15 82.09 Mbp_chr15_82092396_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr15 82.09 Mbp_chr15_82092396_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4__chr14 99.59 Mbp_chr14_99586876_F | TCGTCGGCAGCGTCGCCCTACCTATAGCACACCC | 59.3 | 0.21 |
| mm4__chr14 99.59 Mbp_chr14_99586876_R | GTCTCGTGGGCTCGGCAGGTCCCCGGTAGGAGG | 60.4 | 0.21 |
| mm4__chr2 219.45 Mbp_chr2_219448291_F | TCGTCGGCAGCGTCGAATCCCGACCCCAGACG | 59.5 | 0.20 |
| mm4__chr2 219.45 Mbp_chr2_219448291_R | GTCTCGTGGGCTCGGGAACTGCAGCTTGTCCAGGA | 60.2 | 0.20 |
| mm4__chr1 26.15 Mbp_chr1_26147790_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4__chr1 26.15 Mbp_chr1_26147790_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4__chr7 131.95 Mbp_chr7_131945618_F | TCGTCGGCAGCGTCCGCCTGTCATTGGAGAAGGA | 59.7 | 0.19 |
| mm4__chr7 131.95 Mbp_chr7_131945618_R | GTCTCGTGGGCTCGGCTCTTTCGTCCACCTGGGTC | 60.0 | 0.19 |
| mm3__chr5 82.61 Mbp_chr5_82606028_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3__chr5 82.61 Mbp_chr5_82606028_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm2__chr11 104.76 Mbp_chr11_104758698_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm2__chr11 104.76 Mbp_chr11_104758698_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4__chr2 60.85 Mbp_chr2_60850524_F | TCGTCGGCAGCGTCTGTGTGGACAGCATGGGATG | 60.3 | 0.17 |
| mm4__chr2 60.85 Mbp_chr2_60850524_R | GTCTCGTGGGCTCGGCACCTCCTTTCACATTCCTGC | 59.1 | 0.17 |
| mm4__chr1 1.04 Mbp_chr1_1037017_F | TCGTCGGCAGCGTCGCTTGTCCTGGAAGACCCTC | 60.0 | 0.17 |
| mm4__chr1 1.04 Mbp_chr1_1037017_R | GTCTCGTGGGCTCGGGCAAGACTCGGGTCCTTCTC | 60.1 | 0.17 |
| mm4__chr6 162.94 Mbp_chr6_162943813_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr6 162.94 Mbp_chr6_162943813_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr20 18.20 Mbp_chr20_18202851_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4__chr20 18.20 Mbp_chr20_18202851_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3__chr4 36.53 Mbp_chr4_36529340_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3__chr4 36.53 Mbp_chr4_36529340_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm2__chr10 78.33 Mbp_chr10_78327748_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm2__chr10 78.33 Mbp_chr10_78327748_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4__chr7 149.29 Mbp_chr7_149285534_F | TCGTCGGCAGCGTCCCTCATGGAGGCTTGGGGA | 61.0 | 0.16 |
| mm4__chr7 149.29 Mbp_chr7_149285534_R | GTCTCGTGGGCTCGGGGAGTCCTTGTCCTCCTCCT | 59.9 | 0.16 |
| mm4__chr20 5.38 Mbp_chr20_5383125_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4__chr20 5.38 Mbp_chr20_5383125_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4__chr17 81.17 Mbp_chr17_81165777_F | TCGTCGGCAGCGTCGAGGCCGGTTTGTGCTGG | 61.3 | 0.16 |
| mm4__chr17 81.17 Mbp_chr17_81165777_R | GTCTCGTGGGCTCGGAATTAACCCTTTCCCCGCCC | 60.3 | 0.16 |
| mm4__chr7 148.78 Mbp_chr7_148781769_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4__chr7 148.78 Mbp_chr7_148781769_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4__chr8 41.70 Mbp_chr8_41701861_F | TCGTCGGCAGCGTCCTTCCCATCTCCCCAAACCC | 60.0 | 0.15 |
| mm4__chr8 41.70 Mbp_chr8_41701861_R | GTCTCGTGGGCTCGGGGAGCTTGTCCTCCTTCCAG | 59.7 | 0.15 |
| mm4__chr4 24.77 Mbp_chr4_24773261_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4__chr4 24.77 Mbp_chr4_24773261_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4__chr15 63.94 Mbp_chr15_63941540_F | TCGTCGGCAGCGTCGACAGATGCCTTCTCCCAGG | 59.8 | 0.14 |
| mm4__chr15 63.94 Mbp_chr15_63941540_R | GTCTCGTGGGCTCGGCAGTCAGCTCCCTTGAGTGG | 60.0 | 0.14 |
| mm4__chr7 10.53 Mbp_chr7_10527546_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr7 10.53 Mbp_chr7_10527546_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3__chr4 91.66 Mbp_chr4_91663294_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm3__chr4 91.66 Mbp_chr4_91663294_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4__chr10 78.78 Mbp_chr10_78780000_F | TCGTCGGCAGCGTCAGATGAGGGTGTCAGGGTCA | 59.8 | 0.12 |
| mm4__chr10 78.78 Mbp_chr10_78780000_R | GTCTCGTGGGCTCGGTATGTTCCCGTGTGCTGTCC | 60.0 | 0.12 |
| mm4__chr6 147.51 Mbp_chr6_147508197_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr6 147.51 Mbp_chr6_147508197_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr2 157.75 Mbp_chr2_157750114_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr2 157.75 Mbp_chr2_157750114_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4__chr11 65.79 Mbp_chr11_65790870_F | TCGTCGGCAGCGTCGCGGAAACCTGGCAGATGAT | 60.7 | 0.12 |
| mm4__chr11 65.79 Mbp_chr11_65790870_R | GTCTCGTGGGCTCGGTGATACAAAGGAGCCCCGAC | 59.4 | 0.12 |
| mm4__chr17 43.88 Mbp_chr17_43879958_F | TCGTCGGCAGCGTCGCTGTAACCCTGACCTTCCC | 60.0 | 0.10 |
| mm4__chr17 43.88 Mbp_chr17_43879958_R | GTCTCGTGGGCTCGGTCTGAGGCACTGTAACCTGC | 59.6 | 0.10 |
| mm4__chr2 47.05 Mbp_chr2_47049479_F | TCGTCGGCAGCGTCCTACCCCTGGAGATCTGCCA | 60.3 | 0.10 |
| mm4__chr2 47.05 Mbp_chr2_47049479_R | GTCTCGTGGGCTCGGAAAAGGGCCCGGATGTCAC | 60.3 | 0.10 |
| mm4__chr9 21.81 Mbp_chr9_21813418_F | TCGTCGGCAGCGTCTCCAGAGCAGTAGCCACTCT | 59.9 | 0.10 |
| mm4__chr9 21.81 Mbp_chr9_21813418_R | GTCTCGTGGGCTCGGTCTCAGCAACCCCAAGCTTC | 60.2 | 0.10 |
| mm4__chr7 55.87 Mbp_chr7_55874665_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4__chr7 55.87 Mbp_chr7_55874665_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm2__chr16 58.99 Mbp_chr16_58987256_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm2__chr16 58.99 Mbp_chr16_58987256_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr2 37.70 Mbp_chr2_37699754_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr2 37.70 Mbp_chr2_37699754_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr3 125.81 Mbp_chr3_125813079_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr3 125.81 Mbp_chr3_125813079_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4__chr9 35.23 Mbp_chr9_35225983_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr9 35.23 Mbp_chr9_35225983_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3__chr13 58.63 Mbp_chr13_58626186_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm3__chr13 58.63 Mbp_chr13_58626186_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr5 129.32 Mbp_chr5_129322591_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr5 129.32 Mbp_chr5_129322591_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4__chr12 112.99 Mbp_chr12_112986364_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr12 112.99 Mbp_chr12_112986364_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrX 3.06 Mbp_chrX_3058338_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrX 3.06 Mbp_chrX_3058338_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrX 50.89 Mbp_chrX_50890549_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrX 50.89 Mbp_chrX_50890549_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr3 51.08 Mbp_chr3_51083073_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr3 51.08 Mbp_chr3_51083073_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrY 14.22 Mbp_chrY_14222650_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chrY 14.22 Mbp_chrY_14222650_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4__chr7 178.78 Kbp_chr7_178777_F | TCGTCGGCAGCGTCGTGTGCCCGTCTAAGAACCG | 61.0 | 0.06 |
| mm4__chr7 178.78 Kbp_chr7_178777_R | GTCTCGTGGGCTCGGCGAGGGATCCAGACACAGTG | 59.8 | 0.06 |
| mm4__chr5 137.03 Mbp_chr5_137033152_F | TCGTCGGCAGCGTCTCAGCTCGGCTATGATGCAG | 59.9 | 0.06 |
| mm4__chr5 137.03 Mbp_chr5_137033152_R | GTCTCGTGGGCTCGGAGTCTCCTCTTGCAGATCGC | 59.5 | 0.06 |
| mm4__chr16 89.04 Mbp_chr16_89036565_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr16 89.04 Mbp_chr16_89036565_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr10 46.24 Mbp_chr10_46241262_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr10 46.24 Mbp_chr10_46241262_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4__chr2 196.04 Mbp_chr2_196037023_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr2 196.04 Mbp_chr2_196037023_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3__chr5 171.12 Mbp_chr5_171123557_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3__chr5 171.12 Mbp_chr5_171123557_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr21 5.98 Mbp_chr21_5982552_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr21 5.98 Mbp_chr21_5982552_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr21 43.56 Mbp_chr21_43555996_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr21 43.56 Mbp_chr21_43555996_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chrX 45.58 Mbp_chrX_45576711_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chrX 45.58 Mbp_chrX_45576711_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr12 67.34 Mbp_chr12_67339783_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr12 67.34 Mbp_chr12_67339783_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4__chr8 11.76 Mbp_chr8_11764473_F | TCGTCGGCAGCGTCTCAATGGCTTGTGGGTTCCT | 59.5 | 0.04 |
| mm4__chr8 11.76 Mbp_chr8_11764473_R | GTCTCGTGGGCTCGGTCCTTCCAGGTGTGTGTGTG | 59.8 | 0.04 |
| mm4__chr14 101.47 Mbp_chr14_101471041_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr14 101.47 Mbp_chr14_101471041_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr20 62.42 Mbp_chr20_62418934_F | TCGTCGGCAGCGTCACCCTCTCCTTGCCGTATCT | 60.0 | 0.04 |
| mm4__chr20 62.42 Mbp_chr20_62418934_R | GTCTCGTGGGCTCGGTCTTTCCACTGAGCACCCAC | 59.8 | 0.04 |
| mm4__chr7 72.66 Mbp_chr7_72656002_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 72.66 Mbp_chr7_72656002_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 67.16 Mbp_chr7_67156158_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr7 67.16 Mbp_chr7_67156158_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr3 101.05 Mbp_chr3_101046870_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr3 101.05 Mbp_chr3_101046870_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4__chr2 119.90 Mbp_chr2_119903753_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr2 119.90 Mbp_chr2_119903753_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3__chr12 3.75 Mbp_chr12_3753218_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3__chr12 3.75 Mbp_chr12_3753218_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr5 156.49 Mbp_chr5_156487025_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr5 156.49 Mbp_chr5_156487025_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4__chr17 28.35 Mbp_chr17_28347151_F | TCGTCGGCAGCGTCGGCTGAGTTCCTGTGCTTCT | 59.9 | 0.02 |
| mm4__chr17 28.35 Mbp_chr17_28347151_R | GTCTCGTGGGCTCGGTCACAGTTCCAGCCCTTGTT | 59.4 | 0.02 |
| mm4__chr13 47.55 Mbp_chr13_47553696_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr13 47.55 Mbp_chr13_47553696_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr2 175.34 Mbp_chr2_175343486_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr2 175.34 Mbp_chr2_175343486_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr2 218.26 Mbp_chr2_218257049_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr2 218.26 Mbp_chr2_218257049_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4__chr3 194.80 Mbp_chr3_194797184_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr3 194.80 Mbp_chr3_194797184_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4__chr11 62.60 Mbp_chr11_62602035_F | TCGTCGGCAGCGTCCGGGCTTAACTCTGTCCTCG | 60.1 | 0.00 |
| mm4__chr11 62.60 Mbp_chr11_62602035_R | GTCTCGTGGGCTCGGGGCAAAGCCGCTTGCAAATA | 60.3 | 0.00 |
| mm4__chr5 34.73 Mbp_chr5_34731885_F | TCGTCGGCAGCGTCAAACACTGTGCTTGCACTGG | 59.5 | 0.00 |
| mm4__chr5 34.73 Mbp_chr5_34731885_R | GTCTCGTGGGCTCGGAAAGCCTCCCTGAGCACATG | 60.3 | 0.00 |
| mm4__chr20 62.96 Mbp_chr20_62963020_F | TCGTCGGCAGCGTCCTAGGGCTAAGGCAGACACC | 59.5 | 0.00 |
| mm4__chr20 62.96 Mbp_chr20_62963020_R | GTCTCGTGGGCTCGGCACCTTTGAGAACCGCGTTC | 59.7 | 0.00 |
| mm4__chr16 88.81 Mbp_chr16_88812598_F | TCGTCGGCAGCGTCCAGGTGACCCCTTAGCCTTG | 60.0 | 0.00 |
| mm4__chr16 88.81 Mbp_chr16_88812598_R | GTCTCGTGGGCTCGGGGGGAGTTATGCCTCACACA | 59.3 | 0.00 |
Primers underlined, off-targets in bold.
| ontarget_mm0__chr7 5.53 Mbp_chr7_5529727 |
TGGTGAGCTGCGAGAATAGCCGGGCGCGCTGTGAGCCGAGGTCGCCCCCGCCCTGGCCACTTCCGGCGCGCCGAGTCCTT AGGCCGCCAGGGGGCGCCGGCGCGCGCCCAGATTGGGGACAAAGGAAGCCG |
| mm4__chr18 21.90 Mbp_chr18_21903971 |
GCTGGCCTCTTGTTCACTCATCTCCCTCTCCTCCTAATCTTCTAATAACTTTATTACTGGGAAAAAGGCCAACTAGGGAA AACCCCACTGGCGGCCTGAGGATTCTTGATCTGAAGGGGGCCTTTGAGATCACGG |
| mm4__chr6 98.83 Mbp_chr6_98832745 |
CTGTGTGTGTCCTCGCCTCGCCTCGCCGCGCGGGTGCGCGCGTGGTGGGGGTGCAGGTCCGTCCTGCTGGCGGCCTGTGG GCTCGCCCCTCCCCTATCCATTTCTCTCCCCCACACACGCTCATAAAC |
| mm4__chr14 99.59 Mbp_chr14_99586876 |
GCCCTACCTATAGCACACCCCAAATCCCACCAGGCGGCCTCAGGGCTGGGCACCACAGCCAAGCCCGCAGCCACGGGCGG CACTCCCGCGCTCACAGAGGGCCTCTGCCCTCCTACCGGGGACCTG |
| mm4__chr2 219.45 Mbp_chr2_219448291 |
GAATCCCGACCCCAGACGCCACTGAGCGAGGCCTCAGGCCGCCTGTCGGCGTTGGGCCGATCGCCTAGGCTGGTGCGCGC CGGCTCCCGCATCCTGGACAAGCTGCAGTTC |
| mm4__chr7 131.95 Mbp_chr7_131945618 |
CGCCTGTCATTGGAGAAGGAACCGCGCGGCCGCAGCCCCTTCCTCGCCGACCGCGTCACCTCCTGGCTGCCTGGGGACCC GCGCGGGAACCCCGGAGCAGTAGATTTGTGGAGGTCGACCCAGGTGGACGAAAGAG |
| mm4__chr2 60.85 Mbp_chr2_60850524 |
TGTGTGGACAGCATGGGATGCTCCCTGGGGAGGAGCTCACAGTGAGGAGTCCCTGGGCAGCCAGGGGGCCATCCCAAGAT TGGGGAAAGAAAAGCTAGGAAAGCAGGAATGTGAAAGGAGGTG |
| mm4__chr1 1.04 Mbp_chr1_1037017 |
GCTTGTCCTGGAAGACCCTCTCCCTCCTGGCAGCCCCAGGACTGGGGGCAGGAGGGTATGGACTCAGAGAAGGACCCGAG TCTTGC |
| mm4__chr7 149.29 Mbp_chr7_149285534 |
CCTCATGGAGGCTTGGGGACGTGGAGCCGAGTCCTGAATTCGCCAGGAGGATGTTCCACCCCCCACCATCTCCGGCGACC TCCTGGAACTGGGGAGGGCTCAGCCCGGGTGGCAGGAGGAGGACAAGGACTCC |
| mm4__chr17 81.17 Mbp_chr17_81165777 |
GAGGCCGGTTTGTGCTGGGGCCCAGGGCCTGCCCCTCCGAGATCTGGGGCCGCCAGGGGGTCCGGCTGCTTCTGCTGCTG GGCTCGGGGCGGGGAAAGGGTTAATT |
| mm4__chr8 41.70 Mbp_chr8_41701861 |
CTTCCCATCTCCCCAAACCCCATCCACTCCACTGCTGCCCGGAAAGCTGGTCCCGCCCCTAGTCCTCGGGCCGCTAGGTG GCAGCACAGCCCCGCTGGAAGGAGGACAAGCTCC |
| mm4__chr15 63.94 Mbp_chr15_63941540 |
GACAGATGCCTTCTCCCAGGTCCCCCTGGCTGCCTATGGTCTCTGTGACCTTCCAGCTGGAAACTTCCACTCAAGGGAGC TGACTG |
| mm4__chr10 78.78 Mbp_chr10_78780000 |
AGATGAGGGTGTCAGGGTCAGGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNCCCTTGAGGACAGCACACGGGAACATA |
| mm4__chr11 65.79 Mbp_chr11_65790870 |
GCGGAAACCTGGCAGATGATTTGTCTCAGAAAGAACAGGTGAGGGCGAGTCCTTTGGCTGCAGGGAGGATGGCGAGGGAA GCTGGCCATGGGTGAAGTCGGGGCTCCTTTGTATCA |
| mm4__chr17 43.88 Mbp_chr17_43879958 |
GCTGTAACCCTGACCTTCCCGCTCTGAGTCTTTCGGCCGCCGGGAGGTGTCTAGGGGGATGGGGGTAGGTTGGACCAAAT GGGCAGGGGCAGGTTACAGTGCCTCAGA |
| mm4__chr2 47.05 Mbp_chr2_47049479 |
CTACCCCTGGAGATCTGCCACCCCAGGGGCCTCCGCTGGCTCTTCTGGGGACCCCCTGGCAGCCCAAGGAATGGAAGCCT GAGGCAGGGGGTGACATCCGGGCCCTTTT |
| mm4__chr9 21.81 Mbp_chr9_21813418 |
TCCAGAGCAGTAGCCACTCTTGCTTTCATGTCCAGGGCTACTACATGTTCCTTGCTGAGGGCTGAGTTGCCAGCTACCTG GTGCAGAGTCATTATGCAGCCAGGTGGTGCAGACTGAAGCTTGGGGTTGCTGAGA |
| mm4__chr7 178.78 Kbp_chr7_178777 |
GTGTGCCCGTCTAAGAACCGCGAGGCTTCAGGCCTCCAGGAGGTGTCTTGCGGGGGCAGCCGCTCTGACGTCGCCTCTGC CCACTGTGTCTGGATCCCTCG |
| mm4__chr5 137.03 Mbp_chr5_137033152 |
TCAGCTCGGCTATGATGCAGCTGAATGAGAGCCCAGTGGGGGTCAGGGGAGATGAACTCCCTCGTGGCGGCCGAAGGCCT GGGTGAGGGTGGAGCAGCTGGCGATCTGCAAGAGGAGACT |
| mm4__chr8 11.76 Mbp_chr8_11764473 | TCAATGGCTTGTGGGTTCCTCTGGGTCCTTGGGCCTCCAGGAGGGGCCACTGCCGCACACACACACCTGGAAGGA |
| mm4__chr20 62.42 Mbp_chr20_62418934 |
ACCCTCTCCTTGCCGTATCTTGGGGATCCAACCTGGGCCTGGCTAGCCCCAGCAGCCCCACCTCTCTGGGTTGCCTAGGA GGCAGCCGGGTCCTTGGACCTCCAGGTGGGTGTGGGTGCTCAGTGGAAAGA |
| mm4__chr17 28.35 Mbp_chr17_28347151 |
GGCTGAGTTCCTGTGCTTCTGTTTATCATCTGACCTTGTGTTCTAGCCAGGTCGTAAGCGCGACTGTACTGTTGCCCTGC AACCCCAGCTCCCCCTGGAGGACTAAGGACAAGAATAACAAGGGCTGGAACTGTGA |
| mm4__chr11 62.60 Mbp_chr11_62602035 |
CGGGCTTAACTCTGTCCTCGCTGGAGCCCTGCAGCTCCTCCTCGCGCCCCAAAGACTCGCGCGCCTTAGCGCTTCGCCAG GTTATTTGCAAGCGGCTTTGCC |
| mm4__chr5 34.73 Mbp_chr5_34731885 |
AAACACTGTGCTTGCACTGGCTGGCTGAAGTTTACACTTATTCAAAGCAAGGCAGTTGAGAATTAACCCAAAAACGAATG ACCTGACCTTCCAGGAGTCCTGATGCGGCCAGGGGGCCATGTGCTCAGGGAGGCTTT |
| mm4__chr20 62.96 Mbp_chr20_62963020 |
CTAGGGCTAAGGCAGACACCCAGGAAGACCTGCTGGGCACAGGTCAAGGCAGGGTGCAGGAGCAGCCGAGTCTTTGGGTG GCCAGGGGGCTCTGGAGGAGGCCGTGTGGAGGGTCGTTCAGAACGCGGTTCTCAAAGGTG |
| mm4__chr16 88.81 Mbp_chr16_88812598 |
CAGGTGACCCCTTAGCCTTGGGGTGGGGAGCTGTGAACCCCCTGGCCGCCCGAGGACTGGTACCCAGGGGGTTGTGTGAG GCATAACTCCCC |