CRISPOR

Micro-homology scoring of potential deletions

Guide: CATGTGCAAGGCCGGCTTCG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-". They are ranked by the "micro-homology score". This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

Bae-Score	Sequence	Effect
	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	(Wild-type sequence)
402.0	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGG--------GCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`8 bp deleted → frameshift`
382.8	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGC---------GACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`9 bp deleted → no frameshift`
362.0	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCG--GGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`2 bp deleted → frameshift`
313.2	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGC-------------GACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`13 bp deleted → frameshift`
296.4	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCG------ACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`6 bp deleted → no frameshift`
255.2	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCG---------ATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`9 bp deleted → no frameshift`
229.6	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGG-------------------------GCCGTCTTCCCCTCCATCGT`	`25 bp deleted → frameshift`
223.0	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCG------------------------------TCTTCCCCTCCATCGT`	`30 bp deleted → no frameshift`
219.6	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCG------------ACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`12 bp deleted → no frameshift`
219.6	`GTCGTCGACAACGGCTCCGGCATGTGCAAGG------------GCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`12 bp deleted → no frameshift`
210.0	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCC---------------------CCCCGGGCCGTCTTCCCCTCCATCGT`	`21 bp deleted → no frameshift`
190.2	`GTCGTCGACAACGGCTCCGG-----------------------GCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`23 bp deleted → frameshift`
188.8	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCG---------------ATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`15 bp deleted → no frameshift`
180.6	`GTCGTCGACAACGGCTCCGGC------------------------GACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`24 bp deleted → no frameshift`
170.8	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGC-----------------CCCCCGGGCCGTCTTCCCCTCCATCGT`	`17 bp deleted → frameshift`
163.8	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGC--------------------------CGTCTTCCCCTCCATCGT`	`26 bp deleted → frameshift`
162.8	`GTCGTCGACAACGGCTCCGGCATGTGC------------------GACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`18 bp deleted → no frameshift`
154.8	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCG-------------------GGCCGTCTTCCCCTCCATCGT`	`19 bp deleted → frameshift`
141.0	`GTCGTCGACAACGG-----------------------------GCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`29 bp deleted → frameshift`
136.5	`GTCGTCGACAACGGCTCCGGCATGTGC--------------------------CCCCCGGGCCGTCTTCCCCTCCATCGT`	`26 bp deleted → frameshift`
133.8	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTC------------------------------CCCTCCATCGT`	`30 bp deleted → no frameshift`
133.8	`GTCGTCGACAACGGC------------------------------GACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`30 bp deleted → no frameshift`
133.2	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCC----------------------CCCGGGCCGTCTTCCCCTCCATCGT`	`22 bp deleted → frameshift`
126.8	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCC-----------------------CCGGGCCGTCTTCCCCTCCATCGT`	`23 bp deleted → frameshift`
120.4	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCG------------------------TCTTCCCCTCCATCGT`	`24 bp deleted → no frameshift`
120.4	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCC------------------------CGGGCCGTCTTCCCCTCCATCGT`	`24 bp deleted → no frameshift`
108.0	`GTCGTCGACAACGGCTCCGG----------------------------------------GCCGTCTTCCCCTCCATCGT`	`40 bp deleted → frameshift`
103.6	`GTCGTCGACAACGGCTCCG---------------------------ACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`27 bp deleted → no frameshift`
99.4	`GTCGTCGAC---------------------------------------GATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`39 bp deleted → no frameshift`
98.8	`GTCGTCGACAACGGCTCCGGCATG----------------------------CCCCCCGGGCCGTCTTCCCCTCCATCGT`	`28 bp deleted → frameshift`
94.0	`GTCGTCGACAACGGCTCCGGCATGTGCAAGG-----------------------------GCCGTCTTCCCCTCCATCGT`	`29 bp deleted → frameshift`
89.2	`GTCGTCGACAACGGCTCCG------------------------------ATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`30 bp deleted → no frameshift`
82.5	`GTCGTCGACAACG------------------------------------ATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`36 bp deleted → no frameshift`
80.8	`GTCGTCGACAACGGCTCCGGC--------------------------------CCCCCGGGCCGTCTTCCCCTCCATCGT`	`32 bp deleted → frameshift`
77.7	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTC---------------------------TTCCCCTCCATCGT`	`27 bp deleted → no frameshift`
77.4	`GTCGTCGACAACGGCTCCGGC-----------------------------------------CGTCTTCCCCTCCATCGT`	`41 bp deleted → frameshift`
76.8	`GTCGTCGACAACG---------------------------------ACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`33 bp deleted → no frameshift`
71.0	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTCG---------------------------------------T`	`39 bp deleted → no frameshift`
69.6	`GTCGTCGACAACGGCTCCGGCATGTGC-----------------------------------CGTCTTCCCCTCCATCGT`	`35 bp deleted → frameshift`
69.6	`GTCGTCG-----------------------------------GGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`35 bp deleted → frameshift`
66.0	`GTCGTCGACAACGGCTCC------------------------------------CCCCGGGCCGTCTTCCCCTCCATCGT`	`36 bp deleted → no frameshift`
63.0	`GTCGTCGACAACGGCTCCG---------------------------------------------TCTTCCCCTCCATCGT`	`45 bp deleted → no frameshift`
62.8	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCC-------------------------------------CCTCCATCGT`	`37 bp deleted → frameshift`
62.8	`GTCGTCGACAACGGCTCC-------------------------------------CCCGGGCCGTCTTCCCCTCCATCGT`	`37 bp deleted → frameshift`
61.0	`GTCGTCGA------------------------------------------TGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`42 bp deleted → no frameshift`
60.0	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCC--------------------------------------CTCCATCGT`	`38 bp deleted → frameshift`
60.0	`GTCGTCGACAACGGCTCC--------------------------------------CCGGGCCGTCTTCCCCTCCATCGT`	`38 bp deleted → frameshift`
60.0	`GTCGTCGACAACGGC--------------------------------------CCCCCGGGCCGTCTTCCCCTCCATCGT`	`38 bp deleted → frameshift`
60.0	`GTCGTCGACAACGG----------------------------------------------GCCGTCTTCCCCTCCATCGT`	`46 bp deleted → frameshift`
60.0	`GTCG--------------------------------------GGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`38 bp deleted → frameshift`
57.0	`GTCGTCGACAACGGC-----------------------------------------------CGTCTTCCCCTCCATCGT`	`47 bp deleted → frameshift`
56.8	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCC---------------------------------------TCCATCGT`	`39 bp deleted → no frameshift`
56.8	`GTCGTCGACAACGGCTCC---------------------------------------CGGGCCGTCTTCCCCTCCATCGT`	`39 bp deleted → no frameshift`
52.2	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCTTC-----------------------------------CATCGT`	`35 bp deleted → frameshift`
49.5	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCGGCT------------------------------------CCATCGT`	`36 bp deleted → no frameshift`
48.8	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCC------------------------------------------ATCGT`	`42 bp deleted → no frameshift`
48.8	`GTCG------------------------------------------ACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`42 bp deleted → no frameshift`
42.0	`GTCGTCGACAACGGCTCCGGCATGTGCAAGGCCG---------------------------------------------T`	`45 bp deleted → no frameshift`
42.0	`GTCG---------------------------------------------ATGCCCCCCGGGCCGTCTTCCCCTCCATCGT`	`45 bp deleted → no frameshift`
40.6	`GTCGTCGACAACGGCTCC---------------------------------------------------------ATCGT`	`57 bp deleted → no frameshift`
40.5	`GTCGTCGACAACGGCTCCGGCATGT----------------------------------------CTTCCCCTCCATCGT`	`40 bp deleted → frameshift`
37.0	`GTCGTCGACAACGGCTCC----------------------------------------------------CCTCCATCGT`	`52 bp deleted → frameshift`
35.0	`GTCGTC------------------------------------------------------------TTCCCCTCCATCGT`	`60 bp deleted → no frameshift`
31.2	`GTCGTCGACAACG---------------------------------------------------TCTTCCCCTCCATCGT`	`51 bp deleted → no frameshift`
29.6	`GTCGTCG----------------------------------------------------GGCCGTCTTCCCCTCCATCGT`	`52 bp deleted → frameshift`
28.4	`GTCGTCGACAACGGCTCC-----------------------------------------------------CTCCATCGT`	`53 bp deleted → frameshift`
27.3	`GTCGTCGACAACGGCTCCGGCATGTGCA------------------------------------------------TCGT`	`48 bp deleted → no frameshift`
26.8	`GTCGTCGACAACGGCTCCGGCAT------------------------------------------------------CGT`	`54 bp deleted → no frameshift`
26.8	`GTCGTCGACAACGGCTCC------------------------------------------------------TCCATCGT`	`54 bp deleted → no frameshift`
25.8	`GTCGTCGACAACGGCTC-------------------------------------------------TTCCCCTCCATCGT`	`49 bp deleted → frameshift`
25.6	`GTCG-------------------------------------------------------GGCCGTCTTCCCCTCCATCGT`	`55 bp deleted → frameshift`
23.4	`GTCGTCGACAACGGCT---------------------------------------------------TCCCCTCCATCGT`	`51 bp deleted → no frameshift`
23.2	`GTCGTCG---------------------------------------------------------TCTTCCCCTCCATCGT`	`57 bp deleted → no frameshift`
21.5	`GTC---------------------------------------------------------------TTCCCCTCCATCGT`	`63 bp deleted → no frameshift`
20.0	`GTCGTCGACAACGGCTCCG------------------------------------------------------------T`	`60 bp deleted → no frameshift`
19.2	`GTCGTCGACAACGGCTCCGGCATGT-------------------------------------------------------`	`55 bp deleted → frameshift`
14.8	`GTCGTCGACAACG------------------------------------------------------------------T`	`66 bp deleted → no frameshift`
14.4	`GTCGT---------------------------------------------------------------------------`	`75 bp deleted → no frameshift`
14.1	`GTCGTCGACAACGGCTC-------------------------------------------------------------GT`	`61 bp deleted → frameshift`
13.5	`GTCGTCG------------------------------------------------------------------------T`	`72 bp deleted → no frameshift`
12.9	`GTCGTC---------------------------------------------------------------CCCTCCATCGT`	`63 bp deleted → no frameshift`
11.1	`GTCGTCGACA------------------------------------------------------------------TCGT`	`66 bp deleted → no frameshift`
11.1	`GTC------------------------------------------------------------------CCCTCCATCGT`	`66 bp deleted → no frameshift`
9.9	`GTCGTC--------------------------------------------------------------------CATCGT`	`68 bp deleted → frameshift`
8.7	`GTC-----------------------------------------------------------------------CATCGT`	`71 bp deleted → frameshift`
6.0	`GT------------------------------------------------------------------------------`	`78 bp deleted → no frameshift`

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